46 results about "Equol" patented technology

The invention discloses an ovarian protection composition containing ergothioneine and a preparation method of the ovarian protection composition, and relates to the technical field of ovarian protection compositions. The ovary protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.1 to 0.5 part of the ergothioneine, 0.05 to 1 part of S-equol, 1 to 5 parts of reducing coenzyme Q10, 1 to 3 parts of vitamin E, 10 to 20 parts of chitosan oligosaccharide, 30 to 50 parts of microcrystalline cellulose, 3 to 8 parts of hydroxypropyl methyl cellulose, 0.5 to 2 parts of silicon dioxide and 0.5 to 2 parts of a stabilizer. By adopting the stabilizer, photooxidation and hydrolysis of sensitive components are inhibited through molecular action, and the long-term stability of the composition in a humid and hot environment is remarkably improved in combination with the physical barrier action of silicon dioxide. Through the antioxidant synergistic effect of the ergothioneine and the vitamin E, the hormone regulation effect of the S-equol and the energy metabolism improvement effect of the coenzyme Q10, a multi-layer ovarian function protection system is constructed, and the health state of ovaries is improved. Through a special process, the tablet can be rapidly disintegrated and slowly released in an intestinal targeted manner, burst release of active ingredients is avoided, and the absorption efficiency is improved.

The invention discloses a dihydrodaidzein reductase, a combinatorial enzyme and an application of the dihydrodaidzein reductase and the combinatorial enzyme in synthesis of S-equol. The dihydrodaidzein reductase is a mutant which takes the dihydrodaidzein reductase AeDHDR as a starting sequence and has a corresponding mutation site, wherein the dihydrodaidzein reductase AeDHDR is derived from AdleCRzia equaliforns, and the amino acid sequence of the dihydrodaidzein reductase AeDHDR is shown as SEQ ID NO: 2. The invention also discloses a coding gene, a carrier and a bioengineering bacterium of the dihydrodaidzein reductase mutant and a method for synthesizing S-equol. The method has the advantages of high enzyme activity and high S-equol yield.

The invention relates to the field of gene engineering, in particular to a daidzein reductase mutant and application of the daidzein reductase mutant in preparation of equol, and the daidzein reductase mutant is prepared by performing one or more of the following mutations on the basis of a wild type: V263F, P107A, H351R, G35L, H171A, A267Q, H351Q, M355Q, H351P, G31V, V176A, H351Y, N64K, V137A, H351W, D63A, W257A and C174N. The catalytic activity of the mutant obtained through precise mutation is remarkably improved under the feedback inhibition condition, especially when high-concentration equol exists, the mutant can still keep high relative activity, and continuity and high efficiency of the biosynthesis process are ensured. When the mutant is applied to biosynthesis of equol, the production titer is remarkably improved, the yield can be improved by about 1.2 times compared with that of a wild type, and the economic benefit and the yield of biosynthesis are directly improved.

To provide a method for producing a composition containing equol and / or an equol derivative that does not brown when heated, at least either alone or in the presence of additional amino acids and the like.SOLUTION: The problem can be solved by providing a method for producing a composition, comprising the following steps: (a), (b1) and / or (b2), as well as (c): (a) producing glucose and isoflavone aglycones from isoflavone glycosides; (b1) causing a microorganism capable of producing equol to produce equol from the isoflavone aglycones; (b2) causing a microorganism capable of producing equol derivatives to produce equol derivatives from the isoflavone aglycones; and (c) decomposing the glucose so that the glucose content in the composition is 0 g / L or more but less than 2.0 g / L, or 30 g / kg or less per solid content of the composition.SELECTED DRAWING: None

To manufacture equol simply and reliably. [Solution] The method comprises at least the steps of: dissolving soy germ isoflavones containing daidzin or daidzein, β-glucosidase, and calcined coral calcium hydrogen powder that generates negative hydrogen ions in water to create an aqueous solution of hydrogen-dissolved soy germ isoflavones; mixing the aqueous solution of hydrogen-dissolved soy germ isoflavones for a predetermined time under predetermined temperature and pressure conditions and allowing it to stand; and concentrating equol using an activated carbon filter made of Ina Akamatsu Myotan with a specific surface area of ​​400 m² / g, wherein the temperature conditions are 30°C to 60°C, the pressure conditions are 1 atmosphere to 5 atmospheres, the predetermined time is 9 hours to 15 hours, and the aqueous solution concentrated using the activated carbon filter contains 0.26 g of equol per 100 g.

The present invention is to provide at least a production method of a composition containing equol and / or equol derivative, which does not undergo browning even when heated as it is or in the presence of an additional amino acid or the like. The problem is solved by a production method of a composition including the following (a) process, (b1) process and / or (b2) process, and (c) process. (a) Process of generating glucose and isoflavone aglycone from isoflavone glycoside; (b1) Process of causing a microorganism having equol production ability to produce equol from the isoflavone aglycone; (b2) Process of causing a microorganism having equol derivative production ability to produce equol derivative from the isoflavone aglycone; and (c) Process of decomposing the glucose so that the content of the glucose in the composition is 0 g / L or more and less than 2.0 g / L, or 30 g / kg or less relative to the amount of solid components of the composition.

The disclosure provides a method of reducing occurrence of a neurodevelopmental disorder (ND) in a subject at risk for developing an ND, the method comprising administering a composition comprising an equol-producing bacterium or gut-health-promoting bacterium to the subject.

The application discloses a kind of complex fermentation inoculant for improving equol production, fermentation liquor and application thereof, belong to microbial fermentation technical field.The complex fermentation inoculant of the application, streptococcus thermophilus BLCC2-0025 and lactococcus lactis MP1001 have high β-glucosidase activity, and low activity glycoside form is converted into high activity daidzein;Salivary lactobacillus LS1101 and lactobacillus gregius 0010 have the activity of further converting daidzein into equol;Four strains of bacteria are used in combination, which can synergistically improve the content of equol.The fermentation liquor prepared by fermenting pueraria extract with the complex fermentation inoculant of the application can target regulate intestine-ovary axis, significantly improve the reproductive function of POF mice, relieve ovarian oxidative stress and accurately regulate intestinal flora balance, providing a safe and effective preparation and application scheme for non-hormone intervention of premature ovarian failure.

The invention discloses a glycosyl transferase combined mutant and application thereof, and aims to solve the problems that the existing wild PlUGT43 and F196Y single-point mutants thereof have narrow substrate spectrums and cannot efficiently catalyze a plurality of isoflavone derivatives with large structural differences to perform C-glycosylation reaction. The glycosyl transferase combination mutant provided by the invention is a protein which simultaneously contains five mutated sites, namely T81S, N186S, L187A, F196Y and S310P, on the basis of a wild PlUGT43 amino acid sequence as shown in SEQ ID NO.3. The glycosyl transferase combination mutant provided by the invention is a protein which is obtained by mutating five sites, namely T81S, N186S, L187A, F196Y and S310P. Compared with a wild type and an F196Y single-site mutant, the combined mutant has the advantages that the catalytic activity on classical substrates such as daidzein is improved, and efficient C-glycosylation on various substrates such as soybean dihydroaglycone, equol and resveratrol which are difficult to catalyze or extremely low in catalytic efficiency is also realized; the application potential of the glycosyl transferase in the aspect of synthesis of diversified natural products is improved.

The present invention relates to: an intestinal microbiome biomarker composition for distinguishing equol producers; and use thereof. Intestinal microbiome biomarkers are differently expressed between equol producers and non-producers, and thus equol producers can be distinguished by confirming the expression of the biomarkers. Therefore, it is expected that the intestinal microbiome biomarkers for distinguishing equol producers, of the present invention, can be used to simply distinguish between equol producers and non-producers.

This invention provides *Lactobacillus gasseri* and its application in the preparation of phytoestrogens, relating to the field of biofermentation technology. The *Lactobacillus gasseri* is *Lactobacillus gasseri* (…). Lactobacillus gasseri The strain, KMHD3-7519, registered with the China General Microbiological Culture Collection Center (CGMCC) under number CGMCC No. 32351, can increase the content of phytoestrogens in plant extracts. It promotes the conversion of daidzein to daidzein in plant extracts and simultaneously converts daidzein partially to equol, thus increasing the total amount of phytoestrogens in the extract. This invention provides a probiotic capable of simultaneously promoting the conversion of two phytoestrogens, which can be used to produce daidzein, or equol, or simultaneously increase both daidzein and equol, with broad application prospects.

To provide a new lactic acid bacterium and its use.SOLUTION: The features of the present invention for solving the above problems are as follows. It is characterized in that it can be used for various health-promoting applications using lactic acid bacteria Lacticaseibacillusparacaseisp. Shidare (accession number NITEABP-04138). Specifically, this lactic acid bacterium is characterized by exhibiting various effects such as promotion of production of IgA, IL-10, IL-12, and serotonin, normalization of stool shape and improvement of bowel movement, improvement of feeling of incomplete evacuation, adjustment of intestinal environment, immunoregulatory action, promotion of equol production, and further, promotion of collagen gene and hyaluronic acid gene expression, and EMT suppression.SELECTED DRAWING: None

To provide a food composition containing equol and an organic acid and having improved flavor, especially aroma.SOLUTION: The food composition contains an organic acid and equol, particularly at least one organic acid selected from the group consisting of carbonic acid, hydrogen carbonate, citric acid, lactic acid, phosphoric acid, gluconic acid, acetic acid, malic acid, orotic acid, ammonium salts thereof, alkali metal salts thereof, alkaline earth metal salts thereof, and chelates thereof, and equol.SELECTED DRAWING: None

The present disclosure provides an equol-containing food composition and a method for producing the same.

This invention discloses an S-estrol composition for delaying ovarian aging and its uses, relating to the fields of biomedicine and health products. The S-estrol composition for delaying ovarian aging comprises, by weight, the following components: 1-5 parts S-estrol, 2-8 parts soy isoflavones, 3-10 parts coenzyme Q10, 2-6 parts vitamin E, 4-12 parts vitamin C, 0.01-0.05 parts folic acid, 0.1-0.3 parts zinc source, 0.5-1.5 parts anti-aging protective agent, 50-80 parts carrier, and 10-15 parts purified water. This invention uses high-purity S-estrol as the core, combined with soy isoflavones, coenzyme Q10 and a variety of antioxidant nutrients in a scientific formulation to form a synergistic system for estrogen receptor signal regulation and antioxidant defense. By constructing a complex antioxidant network and introducing novel anti-aging protectants for synergistic effect, the activity of antioxidant enzymes tends to increase and the level of lipid peroxidation tends to decrease, thereby improving the oxidative stress state in ovarian cells as a whole.

The disclosure provides a method of reducing occurrence of a neurodevelopmental disorder (ND) in a subject at risk for developing an ND, the method comprising administering a composition comprising an equol-producing bacterium or gut-health-promoting bacterium to the subject.

The application discloses a tetrahedral framework nucleic acid complex loaded with equol and application of the complex in preparation of a medicine for relieving fatty liver disease. The complex is obtained by stable combination of tetrahedral framework nucleic acid and equol through hydrogen bond and hydrophobic interaction; the equol is loaded on the tetrahedral framework nucleic acid structure, and can be slowly released, so that the bioavailability of the equol is improved, and the problems of poor water solubility and low stability of the equol are effectively overcome. Meanwhile, the complex can inhibit lipid accumulation in HepG2 cells, relieve lipid deposition of liver cells, and reduce accumulation of TG and T-CHO in cells, and can be used for relieving fatty liver, and preparing more and more efficient fatty liver treatment medicines, and has wide application potential in intervention of lipid metabolism disorder and related metabolic diseases.

The invention discloses a method for purifying S-equol in genetic engineering escherichia coli fermentation liquor. According to the method, daidzein is used as a fermentation substrate, and S-equol-producing genetic engineering escherichia coli is used for fermenting escherichia coli BL21-G2 to obtain fermentation liquor; then, according to the characteristic of high polarity of the S-equol, extracting the S-equol generated by fermentation from the liquid phase by using ethyl acetate; and finally, separating and purifying S-equol and other soy isoflavones by using a specific ratio of chloroform and methanol chromatography liquid through a silica gel column, further separating and purifying S-equol and other impurities in the fermentation liquid through an ethyl acetate chromatography liquid, and finally obtaining S-equol with the purity close to 100%. Compared with a traditional ethanol column-passing extraction method, a one-step silica gel column-passing separation method and the like, the method is economical, practical and good in effect, the purity of the obtained S-equol is higher, and the process research prospect is wide.

The invention provides a daidzein reductase mutant as well as a construction method and application thereof, and belongs to the technical field of active enzymes. The invention provides a daidzein reductase mutant. The daidzein reductase mutant is obtained by mutation on the basis of wild daidzein reductase. Compared with a wild type DZNR, the daidzein reductase mutant disclosed by the invention has higher catalytic activity. Moreover, the mutant disclosed by the invention shows stronger tolerance in the presence of high-concentration equol, and the application of the daidzein reductase mutant can promote the preparation efficiency of equol and derivatives thereof.

This invention discloses an Equoluxe multivitamin tablet containing a natural hormone regulator and its preparation process, belonging to the field of pharmaceutical technology. The invention comprises the following formula in parts by weight: 80-100 parts soybean extract, 20-55 parts plant-based lactic acid bacteria, 5-10 parts ginseng extract, 80 parts ginger extract, and 10-30 parts excipients. This invention, through a specific combination, works synergistically to promote estrogen secretion in women, enhance ovarian function, and maintain hormonal balance. By microencapsulating the plant-based lactic acid bacteria during pretreatment, this invention significantly improves the activity retention rate during tableting and storage, achieving an activity retention rate of over 87% after 6 months under accelerated conditions, thus solving the technical problem of poor stability in probiotic products. This invention employs a gradient mixing and low-temperature pressing process to avoid local aggregation of highly active ingredients and high-temperature inactivation, ensuring the uniformity of content and bioavailability of key components such as equoluxone and lactic acid bacteria in the tablets.

Provided is an equol-containing composition in which bitterness is suppressed, and in particular, an equol-containing composition in which bitterness is suppressed and which is completely or equivalently unrestricted in food applications. The present invention provides an equol-containing composition comprising at least one masking agent selected from the group consisting of thaumatin and a siraitia grosvenorii extract, and an equol-containing substance, and if necessary, at least one sweetening agent selected from the group consisting of aspartame, neotame, Advantame, acesulfame, stevioside, curculiginine, molinin, monatin, synsepalum dulcificum protein, and sweet clostridium.

The disclosure provides a method of reducing occurrence of a neurodevelopmental disorder (ND) in a subject at risk for developing an ND, the method comprising administering a composition comprising an equol-producing bacterium or gut-health-promoting bacterium to the subject.

The present invention improves the amount of equol intake of each individual user. This recommended amount output system comprises one or more computers that execute: calculating the amount of equol deficiency of a user by using a predicted value of the amount of equol production of the user and a predetermined target amount; calculating a recommended amount of equol or isoflavone to be ingested by the user on the basis of the calculated amount of equol deficiency and an index value indicating the level of equol production ability of the user; and outputting the calculated recommended amount.

To provide a recommended amount output system, method, and program that improve the equol intake of an individual user.SOLUTION: A system 100 is a recommended amount output system that includes one or more computers (a server 1 and terminals 2A to 2C) that perform the followings: calculating a user's equol deficiency amount using a predicted value of the user's equol production amount and a predetermined target amount; calculating a recommended amount of equol or isoflavone that the user should ingest based on the calculated equol deficiency amount and an index value representing the degree of the user's equol production ability; and outputting the calculated recommended amount. The server includes a processor 11, a storage device 12, and a communication interface (IF) 13, and each terminal includes a processor, a storage device, a communication IF, and an input / output interface (IF) for inputting and outputting information to and from the user. The server and terminals are communicably connected via a network 3.SELECTED DRAWING: Figure 1

The present disclosure addresses the problem of providing a composition that contains 5-hydroxyequol and that can be used in a new application. The present disclosure also addresses another problem of providing a method for extending healthy life expectancy, the method comprising bringing 5-hydroxyequol into contact with a subject.　The present disclosure provides a composition for extending healthy life expectancy, the composition containing 5-hydroxyequol. Also provided is a method for producing a composition containing 5-hydroxyequol, the method comprising producing 5-hydroxyequol from genistein through fermentation. The present disclosure also provides a method for extending healthy life expectancy, the method comprising bringing 5-hydroxyequol into contact with a subject.

This invention belongs to the field of functional food technology, specifically relating to a sleep-aiding composition containing γ-aminobutyric acid (GABA) and its application. The sleep-aiding composition comprises: 2 to 8 parts of fermented soybean extract; 3 to 10 parts of oyster oligopeptide powder; 0.5 to 5 parts of GABA; 1 to 4 parts of Atractylodes macrocephala volatile oil β-cyclodextrin inclusion complex; and 1 to 4 parts of Acanthopanax senticosus extract. This composition is used in the preparation of products with sleep-improving effects. This invention utilizes a two-stage fermentation process to pre-convert soybean isoflavones to estrol and enrich GABA, combined with targeted enzymatic hydrolysis membrane separation to enrich taurine and zinc in oyster oligopeptides to maintain GABA-A receptor sensitivity. The addition of Atractylodes macrocephala volatile oil β-cyclodextrin inclusion complex improves water solubility and stability, achieving stable sleep-aiding effects and minimizing individual differences, while also promoting growth and development.

Provided is a method for producing equol and 5-hydroxyequol using a microorganism having a capability of producing equol from a daidzein compound and a capability of producing 5-hydroxyequol from a genistein compound, wherein the daidzein compound and the genistein compound are present at the start of the culture of the microorganism, the molar conversion efficiency of the genistein compound to 5-hydroxyequol is 0.200% / h or more, the molar concentration ratio of the genistein compound to the daidzein compound at the start of the culture is 1.500 or more or 0.500 or less, the daidzein compound is selected from daidzin, malonyldaidzin, acetyldaidzin, daidzein, dihydrodaidzein, and combinations thereof, and the genistein compound is selected from genistin, malonylgenistin, acetylgenistin, genistein, dihydrogenistein, and combinations thereof.

The invention discloses a mutant for efficiently synthesizing S-equol and a whole-cell transformation system. Specifically, the invention provides a DZNR mutant for catalytic production of S-equol, and the catalytic activity of the DZNR mutant is remarkably improved compared with that of wild type DZNR. By using the engineering bacteria containing the mutant, the yield of S-equol can be obviously improved. Meanwhile, an EcN whole-cell transformation system is constructed and can be used for safely and efficiently generating S-equol.

The present invention pertains to a new lactic acid bacterium Lacticaseibacillus paracasei strain Shidare (accession number: NITE BP -04138). The lactic acid bacterium can be used for various health promotion applications, and specifically exhibits a wide variety of effects such as promotion of production of IgA, IL -10, IL -12, and serotonin, normalization of stool shape, improvement of bowel movement, improvement of stool-remaining feeling, regulation of intestinal environment, immunoregulation action, equol production promotion, and collagen gene and hyaluronic acid gene expression promotion.

To provide a method for producing a composition containing equol and / or an equol derivative that does not brown when heated, at least either alone or in the presence of additional amino acids and the like.SOLUTION: The problem can be solved by providing a method for producing a composition, comprising the following steps: (a), (b1) and / or (b2), as well as (c): (a) producing glucose and isoflavone aglycones from isoflavone glycosides; (b1) causing a microorganism capable of producing equol to produce equol from the isoflavone aglycones; (b2) causing a microorganism capable of producing equol derivatives to produce equol derivatives from the isoflavone aglycones; and (c) decomposing the glucose so that the glucose content in the composition is 0 g / L or more but less than 2.0 g / L, or 30 g / kg or less per solid content of the composition.SELECTED DRAWING: None