8 results about "Daidzin" patented technology

To manufacture equol simply and reliably. [Solution] The method comprises at least the steps of: dissolving soy germ isoflavones containing daidzin or daidzein, β-glucosidase, and calcined coral calcium hydrogen powder that generates negative hydrogen ions in water to create an aqueous solution of hydrogen-dissolved soy germ isoflavones; mixing the aqueous solution of hydrogen-dissolved soy germ isoflavones for a predetermined time under predetermined temperature and pressure conditions and allowing it to stand; and concentrating equol using an activated carbon filter made of Ina Akamatsu Myotan with a specific surface area of ​​400 m² / g, wherein the temperature conditions are 30°C to 60°C, the pressure conditions are 1 atmosphere to 5 atmospheres, the predetermined time is 9 hours to 15 hours, and the aqueous solution concentrated using the activated carbon filter contains 0.26 g of equol per 100 g.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

The invention discloses a glycosyl transferase combined mutant and application thereof, and aims to solve the problems that the existing wild PlUGT43 and F196Y single-point mutants thereof have narrow substrate spectrums and cannot efficiently catalyze a plurality of isoflavone derivatives with large structural differences to perform C-glycosylation reaction. The glycosyl transferase combination mutant provided by the invention is a protein which simultaneously contains five mutated sites, namely T81S, N186S, L187A, F196Y and S310P, on the basis of a wild PlUGT43 amino acid sequence as shown in SEQ ID NO.3. The glycosyl transferase combination mutant provided by the invention is a protein which is obtained by mutating five sites, namely T81S, N186S, L187A, F196Y and S310P. Compared with a wild type and an F196Y single-site mutant, the combined mutant has the advantages that the catalytic activity on classical substrates such as daidzein is improved, and efficient C-glycosylation on various substrates such as soybean dihydroaglycone, equol and resveratrol which are difficult to catalyze or extremely low in catalytic efficiency is also realized; the application potential of the glycosyl transferase in the aspect of synthesis of diversified natural products is improved.

The application discloses a content determination method of Xintong oral liquid, which adopts UPLC method to determine the contents of puerarin, daidzin, 2,3,5,4 ’ tetrahydroxystilbene-2-O-beta-D-glucoside, naringin and icariin in the preparation. The quality detection method is stable and reliable, has strong specificity and good reproducibility, can comprehensively and effectively control the quality of the Xintong oral liquid, is favorable for stabilizing the product quality, ensures the safety and effectiveness of clinical medication, and thus better meets the needs of medical treatment and market.

This invention provides *Lactobacillus gasseri* and its application in the preparation of phytoestrogens, relating to the field of biofermentation technology. The *Lactobacillus gasseri* is *Lactobacillus gasseri* (…). Lactobacillus gasseri The strain, KMHD3-7519, registered with the China General Microbiological Culture Collection Center (CGMCC) under number CGMCC No. 32351, can increase the content of phytoestrogens in plant extracts. It promotes the conversion of daidzein to daidzein in plant extracts and simultaneously converts daidzein partially to equol, thus increasing the total amount of phytoestrogens in the extract. This invention provides a probiotic capable of simultaneously promoting the conversion of two phytoestrogens, which can be used to produce daidzein, or equol, or simultaneously increase both daidzein and equol, with broad application prospects.

This invention relates to the field of biotechnology, specifically to the construction and application of a Benzodiacetic tobacco chassis for synthesizing isoflavones. This invention reconstructs the upstream pathway for isoflavone synthesis in Benzodiacetic tobacco, further identifies and screens key rate-limiting enzymes for isoflavone synthesis in Benzodiacetic tobacco, and utilizes 2A peptides to co-express multiple key genes, further introducing flavonoid synthesis transcription factors to construct a Benzodiacetic tobacco chassis for synthesizing isoflavones. This Benzodiacetic tobacco chassis can efficiently synthesize daidzein and genistein. Furthermore, based on the constructed high-yield Benzodiacetic tobacco platform for daidzein and genistein, various isoflavones can be further synthesized, such as daidzein, genistein, puerarin, gentiopicrin, chickpea extract A, and stigmosiderin, paving the way for the industrialization of plant biosynthesis of various bioactive isoflavones and thus possessing broad application prospects.

This invention discloses a highly efficient method for the enrichment and separation of isoflavones from kudzu root, belonging to the field of traditional Chinese medicine extraction and separation technology. The method includes: adsorbing and enriching kudzu root juice using HP-20 macroporous adsorption resin, optimizing the sample loading and elution conditions, and eluting with 50% ethanol to obtain a crude isoflavone extract; then separating the crude extract using high-speed countercurrent chromatography, employing a solvent system of ethyl acetate-n-butanol-water with a volume ratio of 2:1:3 to achieve efficient and simultaneous separation of four isoflavone components: puerarin, 3′-methoxypuerarin, puerarin apigenin, and daidzein. This invention features a simple process, low solvent consumption, and high separation efficiency, simultaneously obtaining multiple high-purity isoflavone monomers, making it suitable for large-scale production.

The invention discloses a construction method and application of an HPLC (High Performance Liquid Chromatography) specific chromatogram of a leech snake collateral dredging capsule, and belongs to the technical field of quality analysis of traditional Chinese medicine compound preparations. According to the method, nine reference substances including tanshinol, 3 '-hydroxy puerarin, puerarin, 3'-methoxy puerarin, daidzin, calycosin-7-O-beta-D-glucoside, rosmarinic acid, formononin and alkannic acid are selected, a high performance liquid chromatography-mass spectrometry technology is adopted, and the leech snake collateral dredging capsules in different batches are subjected to systematic analysis. The specific chromatogram of the preparation is established. The method established by the invention is high in precision, good in reproducibility and accurate and reliable in result; the method has the advantages that nine characteristic components can be simultaneously identified under a single chromatographic condition, the analysis efficiency is greatly improved, multiple components in a preparation can be synchronously monitored, the method can be incorporated into enterprise quality standards, and the uniformity and the stability of product quality are ensured. The implementation of the invention provides a scientific basis for improving the quality standard of the leech snake collateral dredging capsule, and has an important value for guaranteeing the clinical curative effect and medication safety of the preparation.