10 results about "Stilbene glucoside" patented technology

The invention provides a high-stability black wolfberry fruit lipid-regulating oral liquid and a preparation method thereof, and relates to the technical field of medicines. The semen cassiae and fructus lycii lipid-regulating oral liquid is prepared from semen cassiae ethanol extract, mixed water extract, aromatic water, a flavoring agent, a bacteriostatic agent, a pH regulator and a solubilizer. By optimizing the preparation process, the provided black wolfberry fruit lipid-regulating oral liquid is under the action of the specific flavoring agent, the bacteriostatic agent, the pH regulator and the solubilizer, the solubilizer is polyethylene glycol 400 or a mixture of polyethylene glycol 400 and polyethylene glycol 600, the content degradation speed of stilbene glucoside is obviously slowed down, the validity period of the product is prolonged from 18 months to 24 months, and the stability of the product is improved.

The invention discloses a method for detecting the content of stilbene glucoside in Changchun longevity promoting paste, and belongs to the technical field of content detection of traditional Chinese medicine preparations. The method comprises the following steps: taking 2, 3, 5, 4 '-tetrahydroxystilbene-2-O-beta-D-glucoside as a reference substance, and adding methanol to prepare a reference substance solution; taking the Changchun longevity promoting paste as a test article, adding methanol for weighing, heating and refluxing, cooling, adding methanol for weighing again, shaking uniformly and filtering, and taking the subsequent filtrate to prepare a test article solution; injecting the reference substance solutions with different concentrations into a high performance liquid chromatograph to obtain a reference substance chromatogram, drawing a standard curve, and calculating a regression equation; and injecting the test solution into a high performance liquid chromatograph to obtain a test chromatogram, and obtaining the content of stilbene glucoside according to the peak area and the standard curve. The method has good precision and reproducibility, the content of stilbene glucoside in the Changchun longevity promoting paste can be accurately detected, and the production quality and the product curative effect of the Changchun longevity promoting paste can be effectively controlled.

The invention relates to the technical field of calcium hydroxide, in particular to a preparation process of calcium hydroxide. The preparation process comprises the following steps: S1, raw material preparation: selecting quick lime as a preparation raw material; s2, raw material pretreatment: pretreating quick lime to obtain a base material for later use; s3, preparing an additive, wherein the additive comprises the following raw materials: sodium lignin sulfonate, deionized water, laccase, calcium chloride, alkyl polyglycoside, sucrose fatty acid ester, stilbene glucoside and hydroxypropyl methyl cellulose. According to the invention, the quick lime is mixed by the additive, sodium lignin sulfonate is pretreated by laccase and can be adsorbed on the surfaces of quick lime particles, agglomeration of the particles is prevented by using electrostatic repulsion, alkyl polyglycoside and sucrose fatty acid ester are nonionic surfactants, solid-liquid interfacial tension can be reduced, and dispersion of the particles is further promoted; the calcium chloride can provide seed crystals for the generation of calcium hydroxide, so that fine crystals are finally generated, and the specific surface area of calcium hydroxide is increased.

The application belongs to the technical field of medicine quality control, and relates to a construction method and quality detection method of high-performance liquid chromatography characteristic spectrum of a traditional Chinese medicine preparation for treating chronic liver disease; comprising the following steps: step S1, preparation of reference solution: morroniside control product is weighed, precisely weighed and determined, and 50% methanol is used to prepare the reference solution; preparation of test solution: the prepared traditional Chinese medicine preparation is finely ground, mixed with 50% methanol, ultrasonically treated, and then diluted, filtered and obtained; step S2, high-performance liquid chromatography detection is performed on the reference solution and the test solution respectively, and the characteristic spectrum of the traditional Chinese medicine preparation for treating chronic liver disease is obtained; the detection wavelength is 240 nm; the characteristic spectrum comprises eight characteristic peaks, wherein peak 1 is 5-hydroxymethyl furfural, peak 2 (S) is morroniside, peak 3 is loganin, peak 5 is spinooside, peak 6 is calycosin-7-glucoside, peak 7 is styrine, and peak 8 is salvianolic acid B.

The invention belongs to the technical field of medicine detection, and particularly relates to a method for detecting the content of effective components in seven-ingredient gastrodia elata medicinal liquor, the seven-ingredient gastrodia elata medicinal liquor is prepared from gastrodia elata, codonopsis pilosula, kadsura longepedunculata, poria cocos, fructus lycii, pericarpium citri reticulatae and radix polygoni multiflori preparata, has the effects of tonifying the kidney and soothing the nerves, and is used for neurasthenia, insomnia and dreaminess and the like. The method for detecting the content of the effective components of the seven-ingredient rhizoma gastrodiae medicinal liquor comprises the step of determining the content of gastrodin in a rhizoma gastrodiae medicinal material, the content of stilbene glucoside in a radix polygoni multiflori preparata medicinal material and the content of deoxyschizandrin in a kadsura longepedunculata medicinal material by adopting a high performance liquid chromatography. According to the method, a quantitative analysis method of three main active components is established through high performance liquid chromatography, a quality control system of the seven-ingredient gastrodia elata medicinal liquor is perfected, the adopted detection method is scientific and reasonable, high in specificity, high in accuracy and good in reproducibility, the quality of the seven-ingredient gastrodia elata medicinal liquor can be comprehensively and effectively controlled, and the curative effect and safety of the product are ensured.

The invention provides chelate resin, a preparation method of the chelate resin and application of the chelate resin to purification of regenerated gas of a polycrystalline silicon adsorption tower. The preparation method comprises the following steps: preparation of crosslinked polystyrene microspheres, chloromethylation of polystyrene-divinyl benzene microspheres and grafting of activated stilbene glucoside. The chelating resin and the molecular sieve are matched for use, so that impurities such as B, P and methane in regenerated hydrogen of an adsorption tower in a polycrystalline silicon production technology can be efficiently removed, the accumulation amount of boron, phosphorus and carbon impurities in a large polycrystalline silicon production system along with time change is reduced, and the quality of polycrystalline silicon can be greatly improved.

A method and kit for differentiating cholinergic neurons based on traditional Chinese medicine ingredients is disclosed. The method comprises: using a first culture medium containing a small molecule agent to induce human induced pluripotent stem cells to differentiate into neuroectodermal stage cells; using a second culture medium containing a Hedgehog pathway activator to induce the neuroectodermal cells to differentiate into medial ganglionic eminence cells; and using a third culture medium containing traditional Chinese medicine ingredients to induce the medial ganglionic eminence cells to differentiate into mature cholinergic neurons. The traditional Chinese medicine ingredients include polydatin, paeoniflorin, salidroside, and stilbene glycoside. This method is highly efficient and low-cost while being practical. Cell morphology and marker gene expression confirm that the method results in targeted and efficient differentiation into cholinergic neurons. This provides a more convenient, rapid, and effective cell source solution for various cholinergic neuron-based research.

The application relates to a method for determining the content of stilbene glucoside in Tongbi Huoluo pills by HPLC, which comprises preparation of a test sample solution: (1) after ultrasonic extraction of the sample solution of the Tongbi Huoluo pills to be detected, centrifugal separation is carried out, and the supernatant is extracted with ethyl acetate to obtain an ethyl acetate extract; (2) the ethyl acetate extract is concentrated to near dryness, dissolved with a solvent allowed by the pharmacopoeia, adsorbed with a macroporous adsorption resin, washed with water and / or eluted with water and an alcohol solution in sequence, and the eluate is collected; after removal of the solvent, an extract of the test sample is obtained. The method can be used for detecting the content of stilbene glucoside in the Tongbi Huoluo pills containing 34 traditional Chinese medicines, has high precision, accuracy and recovery rate, is good in system applicability, durability and repeatability, is simple and feasible, and has a wide application prospect.

The application discloses a bacteriostatic, itching-relieving and antioxidant traditional Chinese medicine composition taking a prepared radix polygoni multiflori extract as a main active ingredient, which is composed of the following ingredients in parts by weight: the prepared radix polygoni multiflori extract 15-35 parts, the cypress leaf extract 5-20 parts, the black sesame seed extract 3-12 parts and the ligustrum lucidum fruit extract 3-12 parts. The ultrasonic-assisted cellulase extraction is adopted to greatly improve the dissolution rate of stilbene glucoside in the prepared radix polygoni multiflori and the active ingredient content of the extract is high; the multi-flavor traditional Chinese medicine is compounded to realize the bacteriostasis (staphylococcus aureus and malassezia), itching relief and antioxidant effects in a synergistic manner, the bacteriostasis circle diameter is greater than or equal to 15 mm, and the DPPH free radical scavenging rate is greater than or equal to 75%; the natural traditional Chinese medicine ingredient is mild and non-irritating, is suitable for a scalp care scene, and can be widely used in hair washing and hair care daily chemical products.

This invention discloses a glycosyltransferase PmUGT7 and its application in the biosynthesis of stilbene glycosides. The glycosyltransferase PmUGT7 can efficiently and specifically glycosylate the C2 hydroxyl group of 2,3,5,4'-tetrahydroxystilbene, producing stilbene glycosides in a one-step process. The reaction conditions are mild, the operation is convenient and rapid, the catalytic efficiency is high, and it has strong sustainability.