140 results about "Stilbene glucoside" patented technology

This invention relates to a method for separating and preparing tetrahydroxy distyrene glucoside from Polygonum multiflorum. The method comprises: (1) pulverizing Polygonum multiflorum tubers, sieving to 20 mesh, extracting with water or ethanl, and concentrating the extract; (2) separating the concentrated extract with polyamide or macroporous adsorbent resin chromatographic column, eluting with ethanol, detecting by thin-layer chromatography, and collecting distyrene glucoside component; (3) vacuum-concentrating, preparing saturated solution at room temperature, cooling slowly, evaporating the solvent to precipitate crystals, vacuum-filtering, and recrystallizing to obtain white needle-like crystals of distyrene glucoside. The method has such advantages as easy operation, and high product purity.

The invention discloses a preparation method of stilbene glucoside, which comprises (1), adding polygonum multiflorum thumb with alcohol, heating and refluxing, filtering extract, recovering alcohol in the extract, concentrating until no alcohol smell left, obtaining concentrated solution or extract, (2), adding water into the concentrated solution or extract, mixing uniformly, standing, filtering and extracting the filtrate via acetic ester, (3), recovering acetic ester of extract liquid, concentrating to condensed ointment, drying and breaking to obtain stilbene glucoside. The inventive extract has stilbene glucoside content higher than 55%, with high yield, high product quality and simple process or the like.

The invention discloses a method for preparing a flavonoid glycoside and stibene glucoside type compound by separating from fenugreek. By virtue of a high-speed countercurrent chromatography (HSCCC) separation technology, isomerides such as vitexin and isovitexin and stibene glucoside type compounds such as rhaponticin, deoxyrhapontin and rhapontigenin which have the similar polarity to that of the isomerides in the fenugreek are subjected to separation preparation, so that the separation time is effectively shortened, and the shortcomings of complicated operation, sample die-adsorption loss, low yield and the like of the conventional preparation method are overcome. According to the method, the technology is simple, the reproducibility is high, and the separation efficiency is high; the obtained monomer content is higher than 96.0 percent and can meet the requirements of a type of botanical drugs and standard contrasts; and the method is suitable for industrial production of medical medicines, standard substances, health protection food, health protection medicines and the like.

The invention discloses a method for extracting and separating stilbene glucoside from polygonum multiflorum. The polygonum multiflorum is pulverized and extracted by water or low carbon ethanol percolation; and the extracted liquid is carried out macroporous resin column chromatography, reverse-osmosis concentration, crystallization, recrystallization and freezing for drying to obtain the stilbene glucoside. Compared with the prior art, the invention adopts normal temperature percolation extraction and can prevent the damage to the stilbene glucoside by carrying out extraction at high temperature in addition to have the advantages of less dosage of extracting solvent, few impurity, convenient operation, and the like; the following adopted reverse-osmosis concentration and freezing for drying both adopt low-temperature operation, and the damage to the stilbene glucoside is controlled to the lowest extent; furthermore, the organic acid is added into the concentrated liquid to carry out crystallization and recrystallization, which can change the PH value of a crystallized solution and is beneficial to crystallization, and can favorably protect the stilbene glucoside from being damaged. The purity of the product prepared by the method can reach more than 99 percent (detected by a high performance liquid chromatography (HPLC) method), and the yield of the product can reach more than 5.0 percent.

The invention relates to the field of medicine, and discloses a novel application of stilbene compounds in preparation of drugs for curing ischemia stroke in the acute phase. The effective time windows of rhubarb stilbene glucoside and piceatannol can last for 6 hours; the rhubarb stilbene glucoside and piceatannol can decrease the water content in the rat brain tissues during the cerebral ischemia process of a rat, and improve the rat nerve function scores. The mechanism is that rhubarb stilbene glucoside and piceatannol have strong functions of oxidation resistance and clearance of oxygen free radicals, and have a brain protection function. The piceatannol has stronger functions of oxidation resistance and clearance of oxygen free radicals than those of the rhubarb stilbene glucoside in vitro. So the invention provides applications of rhubarb stilbene glucoside and piceatannol in preparation of drugs for curing ischemia stroke in the acute phase.

The invention relates to a combined extraction and purification technique for multiple active ingredients of a medicinal plant, in particular to a combined extraction and purification technique for multiple active ingredients of polygonum multiflorum. According to the method, stilbene glucoside, rheum emodin and chrysophanol in the polygonum multiflorum can be maximum extracted from the polygonum multiflorum into a solvent by using 70-80 percent of ethanol as an extraction solvent and are separated from starch in residual slag; water-soluble differences of the stilbene glucoside, the rheum emodin and the chrysophanol are used and water is used as a solvent so that the stilbene glucoside in alcohol extracts can be separated from the rheum emodin and the chrysophanol, and a crude product is obtained; the rheum emodin and the chrysophanol can be respectively separated from the alcohol extracts by successively using 5 percent of sodium carbonate and 5 percent of sodium hydroxide as solvents, and the rheum emodin and chrysophanol crude product can be obtained through an acid precipitation; and the polygonum multiflorum ethanol extract residual slag is washed by using water, and the starch with a purity of 85-87 percent can be obtained through precipitating water washing liquid. The invention has the characteristics of low cost, high efficiency, no pollution and high product purity.

The invention belongs to the technical field of analysis and detection, and discloses a capillary electrophoresis method for simultaneously separating and detecting stilbene glucoside and anthraquinone component in polygonum multiflorum. The method adopts micellar electrokinetic capillary chromatography to analyze and detect polygonum multiflorum extracting solution to obtain the electrophoresis pattern of stilbene glucoside, emodin, aloeemodin, rhein, chrysophanol or physcion; according to a correction curvilinear equation, the concentration of stilbene glucoside, emodin, aloeemodin, rhein, chrysophanol or physcion is calculated. The invention can simultaneously analyze and detect the stilbene glucoside and anthraquinone component in polygonum multiflorum and is simple, quick and efficient; adopted buffer liquid is mainly aqueous solution, does not need a great quantity of organic solvent and has small pollution and environment protection; a small amount of needed solvent and sample is needed, and analysis cost is low.

The invention relates to a quality control method for soft capsule preparation for kidney tonifying, which comprises distinguishing tuber of multiflower knotweed, Chinese angelica root and psoralea fruit, and measuring content of diphenyl ethylene glycosides.

The invention relates to Brefeldin A-producing bacteria, a preparation method, a method for preparing the brefeldin A by fermentation and the application thereof. The Brefeldin A-producing bacteria has the advantages of easy selection, cultivation and storage, low cost, simple process, short preparation period, high yield, no usage of toxic raw material, less organic solvent, thorough guarantee of medicine resource, potential of mass production and favorable economic and social benefits, contributes to the maintenance of ecological balance and the protection of natural resources. The fermentation product of the invention, of which the main component is THSG, develops novel medical application, the Brefeldin A has high safety, low toxicity, stable property, stronger pharmacological action, simple preparation process, high yield and considerably wide range of application, is multipurpose, and can provide a novel source for medicines for preventing, diagnosing, detecting, protecting, curing and researching antimycotic, antiviral and antineoplastic diseases and the directly related diseases.

The invention discloses an external healthcare product for promoting hair growth, which is characterized by being prepared by the following steps of: weighing 10-20g of total saponins of ginseng, 15-35g of astragaloside, 10-20g of glycyrrhizia polysaccharide, 15-45g of kushenin, 2-5g of capsaicin and 35-70g of total stilbeneglycoside of polygonum multiflorum respectively; sufficiently stirring and dissolving the components in 65-75% ethanol solution; standing for 12-24 hours; and filtering to obtain a liquid product. The external healthcare product disclosed by the invention can quickly promote hair growth in one month.

The invention provides a separation and purification process of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside (short for stilbene glucoside). The separation and purification process comprises the steps of weighting a stilbene glucoside extractive, stirring a sample by using silica gel until the sample is loosened, eluting the loosened sample on a glass column by using an eluent, tracking and checking through a thin layer chromatography (TLC), merging a stilbene glucoside contained component, recovering a solvent, and drying to obtain the stilbene glucoside extractive; adding pure water, heating to dissolve the stilbene glucoside extractive, then, freezing and crystallizing the solution, carrying out suction filtration after the frozen and crystallized solution is molten, and washing the solution with the pure water to obtain a white crystal; transferring filter residues to a vacuum drying oven for drying to obtain a pure product of stilbene glucoside. The process is simple and feasible in operation, capable of improving the product purity, and capable of quickly separating and purifying a large amount of stilbene glucoside. The process is simple and convenient to operate, low in cost, stable in yield, capable of obtaining high-purity stilbene glucoside after re-crystallization treatment while keeping low cost and high yield, and suitable for meeting the requirements for large-scale industrial production.

The invention relates to a method for processing Polygonum multiflorum by utilizing fungi fermentation. The inventive method comprises the following steps: slicing or pulverizing Polygonum multiflorum, putting in a container, adding water for fully humidification or preparing into pulp, innoculating strain of cultured Mucor or Rhizopus oryzae, and culturing at 15-37deg.C for 3-7 days. Technical matters for solving in the invention is to degrade nocuous anthraquinone in Polygonum multiflorum through microorganism fermentation method and effectively reserve stilbene glucoside, thereby in effective reservation of pharmacological action of Polygonum multiflorum removing noxious constituent of Polygonum multiflorum and improving medicinal value of Polygonum multiflorum.

The invention discloses a separation and purification process for extracting tetrahydroxy stilbeneglycoside from roots and stems of Polygonum multiflorum. The process comprises the following steps of: firstly, extracting the Polygonum multiflorum by ethanol to obtain a leaching solution; removing an ethanol solvent to obtain a brown extract; adding ethyl ether into the brown extract to carry out cold soaking treatment and removing an ether layer; adding water into an extract obtained by the cold soaking treatment to dissolve; utilizing ethyl acetate to extract and collect an ester layer; removing a solvent to obtain a brown crude product; adding alkali liquid into the crude product to adjust a pH (Potential of Hydrogen) to 8-10; then utilizing the ethyl acetate to extract; adding cyclohexane and ethanol into an extracted water layer; carrying out rotary evaporation to remove the solvent to obtain a TSG (TetrahydroxyStilbene Glucoside) salt crude product; and acidifying and extracting by the ethyl acetate to obtain a pure product. According to the separation and purification process disclosed by the invention, various complicated column chromatographs are avoided and a synthesis process is greatly simplified; and whole process conditions are moderate, and a toxic and harmful solvent is not used, so that the separation and purification process is suitable for industrial production.

The invention relates to a novel 2,3,5,4'-tetrahydroxy stilbene-2-O-beta-D-glucoside crystal and a preparation method thereof as well as a medicine composition containing the crystal. The content of the crystal is 90%-99.9% through the measurement of a high performance liquid chromatography. The preparation method the high-purity stilbene glycoside crystal comprises the steps of Polygonum multiflorum extraction, organic solvent extraction, column chromatography separation, crystallization in a proper solvent and the like. The preparation method provided in the invention has the advantages of high yield and low cost and is suitable for industrialized production.

A method for measuring contents of 14 chemical components in a traditional Chinese medicine composition is disclosed. HPLC-MS/MS is adopted to simultaneously measure the contents of the 14 chemical components in the composition. The composition is a pulse-invigorating heart-tonifying pill. The 14 chemical components include emodin, lobetyolin, 2,3,5,4'-tetrahydroxy stilbene-2-O-beta-D-glucoside, glycyrrhizic acid, liquiritin, liquiritigenin, polydatin, rutoside, isoliquiritigenin, ononin, verbascoside, gallic acid, schizandrin, and rhein. The method includes building standard curves of the 14chemical components; acquiring multi-reaction ion chromatograms of the 14 chemical components in the pill; and determining the contents of the 14 chemical components in the pill. The method has characteristics of high sensitivity, high reliability, a high efficiency and capability of being rapid. Through measuring the contents of the 14 chemical components in the pill, quality control on the pillcan be more effectively performed, thus ensuring clinical treatment effects of the pill.

The invention discloses an organic and inorganic compound fertilizer for polygonum multiflorum. The organic and inorganic compound fertilizer comprises organic fertilizer and inorganic fertilizer. The inorganic fertilizer comprises urea for nitrogenous fertilizer, calcium magnesium phosphate fertilizer for phosphate fertilizer and potassium sulfate for potash fertilizer. The organic and inorganic compound fertilizer is prepared from, by weight parts, 578-584 parts of organic fertilizer, 98-102 parts of urea, 238-244 parts of calcium magnesium phosphate fertilizer and 108-112 parts of potassium sulfate, and the organic and inorganic compound fertilizer is prepared from mixing uniformly the raw materials. Earthworm metabolic cow dung organic fertilizer is utilized by the organic fertilizer. The formula organic and inorganic compound fertilizer is subjected to scientific fertilizer application for the polygonum multiflorum, can reduce fibrous roots of the polygonum multiflorum, improve the utilization rate of the fertilizer and improve soil physico-chemical properties, so that the content of tetrahydroxystilbene glucoside and combined anthraquinone of the polygonum multiflorum is improved greatly, the high yield and high quality polygonum multiflorum is obtained, and the production cost is reduced greatly. The organic and inorganic compound fertilizer has the advantages of being high in utilization rate, stable in performance, easy to use and free of wetting and hardening phenomena, and the organic and inorganic compound fertilizer is favorable for popularization and application of a large area.

The present invention discloses application of bilobalide as a synergist in preparation of a drug or a heath product for preventing cranial nerve injury diseases. An inventor discovers that compounds of bilobalides, particularly bilobalide, bilobalide extracts, and hydroxyl derivatives of bilobalides, particularly glucoside derivatives, ester derivatives and ether derivatives can well promote drug molecules with a treating or health care function on cranial nerve injury diseases to enter brain tissues, wherein the drug molecules comprise ginsenoside, stibene glucoside, resveratrol, levodopa, edaravone, vinpocetine, nicergoline, citicoline, oxiracetam and the like; under the conditions of not increasing drug concentration in blood, the concentration of bilobalide can be increased to a large extent in the brain tissues, so that the efficacy of the drug can be improved, and side effects and adverse reactions of the drug can be reduced.

The invention discloses a preparation method of tuber fleeceflower root formulation granules. The preparation method comprises the following processing steps of: selecting the tuber fleeceflower root as a raw material, rinsing, drying to soft at 60 DEG C, slicing while hot, and drying at 55 DEG C to obtain tuber fleeceflower root slices for decoction; decocting the slices in water, centrifugally separating, concentrating the separated liquid under reduced pressure to obtain an extract with 1.04-1.10 relative density; carrying out spray-drying on the extract to prepare a dry extract power; adding dextrin into the extract powder, uniformly mixing, adjusting the weight content of stilbene glycoside to be not less than 2.52%, preparing a soft material by using 80% ethanol, extruding, granulating and drying to obtain the target drug. The preparation method is a water extraction process, the imitated decoction characteristics of the formulation granules are fully considered so that the stilbene glycoside type and anthraquinone glycoside type compounds have high dissolution rate, and the curative effect of the formulation granules is exerted.

The invention provides an application of stilbene glucosides in preparing drugs for treating and preventing AIDS. Structural formulas of the stilbene glucosides are show as a general formula (I), wherein R1, R2, R3, R4, R5 or R6 independently represent hydrogen, hydroxyl, C1-C4 alkyl, C1-C4 alkoxy, C1-C4 acyloxy, halogen, nitryl, trifluoromethyl or a cyano group, and pre-conditions are that at least one of R1, R2 and R3 is the hydroxyl, at least one of R4, R5 and R6 is the hydroxyl, and at least one hydroxyl of R1, R2, R3, R4, R5 and R6 is connected with sugar. In the compounds represented by the general formula (I), an inhibitory activity (IC50) for the HIV-1 protease of 2,3,5,4-tetera-hydroxystilbene-2-O-beta-D-glucoside of the stilbene glucosides for reaches 126 [mu]M.

The invention discloses novel application of stilbene glycoside to the field of analgesic medicine, and particularly discloses application of stilbene glycoside to preparing medicine for enhancing analgesic effects of opioid medicine and delaying or relieving opioid tolerance. The application has the advantages that the stilbene glycoside is used with the opioid medicine, or compound preparations are made of the stilbene glycoside and the opioid medicine, so that the analgesic time can be prolonged, the analgesic efficacy can be enhanced, and opioid tolerance during chronic opioid medicine treatment procedures can be delayed or relieved; excessive activation of spinal glial cells induced by the opioid medicine is particularly suppressed by the stilbene glycoside, so that effects can be realized.

The invention provides a method for separating and purifying stilbene glucoside monomers, which adopts high-speed counter-current chromatography and uses a ternary solvent system composed of ethyl acetate, ethanol and water as a separation solvent system to separate and extract the stilbene glucoside monomers from a crude stilbene glucoside product. The separating and purifying method of the invention adopts a semi-preparative high-speed counter-current chromatograph to separate and purify the crude stilbene glucoside product, allows the crude stilbene glucoside product to go through the ternary solvent system, and utilizes the characteristics of high recovery rate and no irreversible adsorption of the high-speed counter-current chromatography to massively prepare the stilbene glucoside monomers, and the preparation amount can reach a gram magnitude; and the purity of the prepared stilbene glucoside monomers, which is high, reaches above 99%, and a standard sample of the stilbene glucoside monomers can be prepared with the high purity stilbene glucoside monomers as a raw material. The method of the invention is suitable for different crude stilbene glucoside products, and has the advantages of advanced technology, high separation efficiency, good recovery rate, high product purity, large preparation amount and the like.