44 results about "Specnuezhenide" patented technology

The invention relates to a method for extracting specnuezhenide crude products (higher than 80 percent) and high-purity specnuezhenide (higher than 98 percent) from fructus ligustri lucidi, which belongs to the field of natural medicine chemistry. The fructus ligustri lucidi is used as a raw material, medicine materials are firstly crushed, a water decoction extraction method is selected, extracting solution is rarefied by macroporous resin columns after being slightly concentrated, then, the alcohol deposition treatment is carried out, and finally, the positive silica gel column purification and the opposite silica gel column purification are selected for preparing the specnuezhenide with different content requirements. The method can be used for preparing the specnuezhenide with lower content in a scale way, the yield is high, the operation is simple, and the cost is low; and the specnuezhenide with higher content can also be prepared, the yield is lower, the cost is higher, the selection can be carried out according to the content requirement, raw material parts and reagent parts during the preparation by the two method can be repeatedly utilized, and the preparation cost is lower than that of the existing preparation method.

The invention discloses a method for determining specnuezhenide content in a Zhenqifuzheng preparation. Taking specnuezhenide standard as contrast, the method can determine the specnuezhenide content in the Zhenqifuzheng preparation by high-performance liquid chromatography by selecting a solution containing methanol and a mixed solution with a ratio of (20-50):(80-50) or a mixed solution containing acetonitrile and the mixed solution with a ratio of (5 to 35):(95-65), wherein the mixed solution consists of water, 0.01% to 5% of phosphoric acid water solution, 0.01% to 5% of glacial acetic acid, 0.01% to 0.5% of methanoic acid water solution. Compared with the prior art, the method provided by the invention has the advantages of high specificity, high precision, high repeatability, high yield, high stability, and accurate result by adopting high-performance liquid chromatography, and can effectively control the preparation quality, thereby ensuring the stability of product quality as well as clinical safety and effectiveness.

The invention provides a detection method of a traditional Chinese medicinal composition for treating waist and knee pains and sciatica. Raw materials of the traditional Chinese medicinal composition comprise Rhizoma Cibotii, Fructus Rosae Laevigatae, Caulis Spatholobi, Philippine flemingia root, Kadsura coccinea, Millettia specisoa Champ, Ligustri Lucidi Fructus, Chinese Taxillus Twig, semen cuscutae, Rhizoma Corydalis, Zanthoxylum nitidum, Boswellia carteri and myrrh. The detection method comprises the following steps: detecting the content of (2'S)-Kadsura longepedunculata lignin J in the Kadsura coccinea through high performance liquid chromatography; and detecting the content of specnuezhenide in the Ligustri Lucidi Fructus through the high performance liquid chromatography, and detecting the Kadsura coccinea, the Rhizoma Cibotii and the Chinese Taxillus Twig through thin layer chromatography. The invention also concretely discloses conditions and concrete operating steps of all above detection technologies. The detection method allows five raw materials to be qualitatively and quantitatively detected, and the quantity of the detected raw materials is 1/3 of the total quantity of the raw materials, so the quality of medicines is effectively guaranteed, and medical demands are met.

The invention provides application of specnuezhenide in preparation of medicine for treating neovascular diseases. The applicant discovers from a lot of researches that specnuezhenide has a remarkable inhibition function on vascular endothelial growth factors (VEGF), can remarkably improve hypoxia-induced pathological phenomena like circuity, expansion and deformation of retina new vessels, and can decrease the occupied area of the new vessels, so that specnuezhenide can effectively inhibit formation of eye new vessels and has the function for treating neovascular diseases. The invention provides application of specnuezhenide in preparation of medicine for treating diseases relevant to HIF-1 alpha high expression.

The invention provides a method for preparing a specnuezhenide reference substance. The preparation method specifically comprises the following steps: (i) providing specnuezhenide alcohol extract; (ii), mixing the specnuezhenide alcohol extract and a solvent to obtain a first mixture, and performing column chromatography separation on the first mixture by virtue of an intermediate-pressure preparation column, thereby obtaining a crude specnuezhenide product; (iii) performing recrystallization on the crude specnuezhenide product by virtue of purified water; (iv) separating and drying crystals obtained in step (iii), so as to obtain the specnuezhenide reference substance. The method is stable in process, convenient to operate, high in separation efficiency, low in cost, economical and environment-friendly, and has relatively high economic value and academic value.

The invention discloses a compound traditional Chinese medicine extract preventing glucose metabolism disturbance, comprising the following effective ingredients: dried alcohol, Beta-sitosterol, hexacosanoic acid, butenolide III, oleanolic acid, berberine, jateorhizine, coptisine, salvianic acid A, salvianolic acid B, ring-tetracosane, 9,12-octadecadienoic acid, 5,7-dimethoxy coumarin, specnuezhenide, ginsenoside Rb1 and Rg1, notoginsenoside R1 and encommiol. A preparation method of the extract is as follows: extracting raw material medicines by C1-3 alcohol and/or water, combining a total extract, extracting the total extract by organic solvents with different polarities so as to obtain all effective ingredients, and finally mixing the ingredients so as to obtain a product. As for the compound traditional Chinese medicine extract, a large number of ineffective chemical ingredients in Chinese medicine are removed, so that the content of effective ingredients is increased greatly, and the influence on product processing and preparation quality caused by the ineffective ingredients is reduced; and simultaneously, the preparation process is stable, the product quality is controllable, the mass production is facilitated, and the drug effect of the compound traditional Chinese medicine is improved.

The invention relates to the technical field of medicines, and discloses a preparation method of total iridoid glycoside of ligustrum lucidum ait and application of the total iridoid glycoside of ligustrum lucidum ait to preparation of an anti-osteoporosis medicine. A ligustrum lucidum ait medicinal material is subjected to thermal extraction by using hydrous ethanol to obtain an extracting solution; the extracting solution is purified by adopting macroporous adsorption resin to obtain a total iridoid glycoside extract of the ligustrum lucidum ait. The total iridoid glycoside of the ligustrumlucidum ait contains 19.67 weight percent of specnuezhenide, 0.95 weight percent of oleuropein, 36.44 weight percent of nuezhenoside and 9.01 weight percent of oleonuezhenide. The extract can promoteformation of osteocytes and suppress bone absorption of the osteocytes; under the action of multiple relevant indexes for bone metabolism, the anti-osteoporosis effect is enhanced; the aim of preventing and treating osteoporosis is fulfilled; the total iridoid glycoside of the ligustrum lucidum ait can be used for preparing a medicine for preventing and treating the osteoporosis.

The invention relates to a molecular traditional Chinese medicine sustained-release tablet for resisting bone mass loss and a preparation method thereof. The molecular traditional Chinese medicine sustained-release tablet comprises molecular traditional Chinese medicine ingredients and pharmaceutically allowed auxiliary material ingredients, wherein the molecular traditional Chinese medicine components comprise 1-5 parts of specnuezhenide (ligustrum lucidum), 1-5 parts of wedelolactone (eclipta prostrata), 0.5-3 parts of paeoniflorin (peony), 0.5-3 parts of psoralen (psoralea corylifolia), and 1-5 parts of diosgenin (yam) ]; and the auxiliary material ingredients comprise 10-50 parts of sodium alginate, 1-5 parts of carbomer (934) , 10-30 parts of hydroxypropyl methyl cellulose, 10-40 parts of lactose, and 1-5 parts of magnesium stearate. Based on a theory of Chinese medicine and a theory of sustained-release preparations, Chinese medicine pathological features of postmenopausal osteoporosis, and scientific compatibility, the molecular traditional Chinese medicine sustained-release tablet which is safe and reliable, high in bioavailability and significant in curative effect is developed.

The invention discloses a thin layer chromatographic detection method for a traditional Chinese medicine composition. The thin layer chromatographic detection method comprises the following steps: a, respectively preparing a solution for test products; b, preparing a reference solution; c, applying a sample of each of the solution for test products, the reference solution of calycosin-7-glucoside and the reference solution of specnuezhenide on a same GF254 thin layer plate, developing by using chloroform-methanol-water as a developing solvent, taking out and then air drying; d, placing under an ultraviolet lamp (254nm) for inspection. According to the thin layer chromatographic detection method for the traditional Chinese medicine composition for improving immunity of animals, provided by the invention, astragalus membranaceus and fructus ligustri lucidi in the traditional Chinese medicine composition are both detected simultaneously, the detection result is accurate and reliable, and at the same time, the thin layer chromatographic detection method has the advantages of less process, high convenience in operation, short detection time and low cost and the like, and is particularly suitable for industrial application.

The invention discloses a detection method of giant knotweed rhizome and glossy privet gout capsules. According to the invention, contents of polydatin, plantamajoside, specnuezhenide, and polydatin are determined by using a high performance liquid chromatography method. The method comprises the steps of sample solution preparation, control solution preparation, and determination. With the detection method provided by the invention, the contents of the four components of polydatin, plantamajoside, specnuezhenide, and polydatin can be simultaneously detected under a same chromatographic condition, such that operation steps of content determinations of the four components can be effectively simplified, determination time can be reduced, working efficiency can be improved, organic solvent application can be reduced, and effective control over the quality of giant knotweed rhizome and glossy privet gout capsules can be realized. The detection method has high sensitivity, good separation degree, and specificity. Accuracy, reproducibility, linear relationship, and stability of the method reach requirements of scientific researches and productions, such that the method can be popularized.

The invention provides a quality control method of a ligusticum wallichii shampoo extract. The method adopts thin layer chromatography to identify glossy privet fruit, eclipta and gallnut therein, andmeasures the specnuezhenide by high performance liquid chromatography. The quality detection method of the ligusticum wallichii shampoo extract can be used for identifying glossy privet fruit, eclipta and gallnut and measuring the content of specnuezhenide, and can also be used for qualitatively identifying the ligusticum wallichii shampoo water extract and measuring the content of main components. The method is simple and convenient, good in repeatability and reliable in result, provides a method for quality control of the ligusticum wallichii shampoo, guarantees safety and effectiveness ofproducts, and has good application prospects.

The embodiment of the invention discloses application of specnuezhenide in preparing an autophagy inducer or a pharmaceutical composition. By means of discovery of the autophagy inducing effect of thespecnuezhenide, the specnuezhenide is expected to the developed into the novel autophagy inducer to be used for disease treatment or auxiliary treatment, such as auxiliary treatment of bacterial infection diseases and auxiliary treatment of tumors.

The invention relates to the technical field of traditional Chinese medicine, in particular to a method for preparing specnuezhenide from glossy privet fruits. According to the method, the glossy privet fruits are used as raw materials; backflow extraction is performed through hydrous ethanol; extraction liquid is subjected to filtering concentration and is then purified twice by macroporous resin; then, crystallization is performed through ethanol; a specnuezhenide compound is prepared; the purity reaches 98.2 percent or higher. The method has the advantages that the raw materials are rich and can be easily obtained; the process is simple; the implementation is easy; the operation is simple and convenient; the efficiency is high; the environment is protected; the production cots is lower;the method is applicable to industrial production.

The invention discloses nighttime frequent micturition acting medicinal liquid. The medicinal liquid comprises, by weight, 12 parts of the dried fruit of rubus chingii Hu, 12 parts of the seed of Chinese dodder, 12 parts of the fruit of Chinese wolfberry, 12 parts of the dried fruit of rosa laevigata, 12 parts of the fruit of glossy privet, 15 parts of the root of astragalus mongholicus, 12 partsof the root of salvia miltiorrhiza, 12 parts of the dried root of Radix paeoniae Alba, 5 parts of honey-fried licorice root, 15 parts of the prepared tuber of polygonum multiflorum, 24 parts of herbaepimedii and 1 part of the dried bark of cinnamomum cassia; the content of icariin of every bottle of the medicinal liquid is no less than 6.0 mg, and the amount of specnuezhenide is no less than 5.0mg. The invention also provides a preparation method for the nighttime frequent micturition acting medicinal liquid. By means of the nighttime frequent micturition acting medicinal liquid and the preparation method thereof, liquid precipitation can be reduced, the content of active ingredients of the icariin, the specnuezhenide and the like is effectively improved, and curative effects are improved.

The invention discloses an application of a membrane separation technology in the preparation process of a wine-fried Ligustri Lucidi Fructus and Yerbadetajo Herb immunity-enhancing oral liquid. The membrane separation technology is applied to the preparation process of the wine-fried Ligustri Lucidi Fructus and Yerbadetajo Herb immunity-enhancing oral liquid, immunity-enhancing water extract of wine-fried Ligustri Lucidi Fructus and Yerbadetajo Herb undergoes membrane filtration impurity removal and membrane concentrating in a manner of combining microfiltration, ultrafiltration and nanofiltration by using the principle of intercepting different molecular weights by using different membrane modules, and the optimization of the impurity removal process ensures the stability of the oral liquid; and compared with traditional water extraction and alcohol precipitation technologies, the technology in the invention provides a reference for the applicability of the technology applied to theimpurity removal process of the immunity-enhancing water extract of Wine-fried Ligustri Lucidi Fructus and Yerbadetajo Herb. Experimental results show that the transfer rates of Specnuezhenide and icariin in the wine-fried Ligustri Lucidi Fructus and Yerbadetajo Herb immunity-enhancing oral liquid prepared by the membrane separation technology is 70% or above, the Specnuezhenide content and the icariin content of the prepared the wine-fried Ligustri Lucidi Fructus and Yerbadetajo Herb immunity-enhancing oral liquid are higher than those of extract prepared by the traditional alcohol precipitation technologies, and the production cost is saved, so the traditional water extraction and alcohol precipitation technologies can be replaced.

The invention relates to the field of near-infrared detection, in particular to an establishment method for a near-infrared quantitative correction model of Zhenqi Fuzheng Preparations and a detectionmethod for Zhenqi Fuzheng Preparations. The invention establishes NIR models of salidroside, calycosin-7-glucoside, Specnuezhenide, astragaloside A, and moisture index of Zhenqi Fuzheng Preparation finished products, which can simultaneously and quickly predict contents of multiple indexes of the Zhenqi Fuzheng Preparations without cumbersome sample processing, and prediction results are accurate, thereby satisfying detection requirements for Zhenqi Fuzheng Granule samples in the production. The method established in the invention not only shortens a detection period, reduces the use of consumables, and reduce detection costs of the Zhenqi Fuzheng Granules, but also enable on-site analysis. In the follow-up, the sample number will be further increased, the coverage of the samples will beexpanded, and a more stable model can be established to provide a fast and efficient detection method for the quality control of Zhenqi Fuzheng Granules.

The invention relates to a method for determining specnuezhenide content through high performance liquid chromatography. The method comprises the following steps: 1) extracting specnuezhenide from a to-be-tested sample powder containing specnuezhenide with an aqueous solution of methanol having a volume fraction of 55-65% so as to obtain a to-be-tested solution; 2) introducing the to-be-tested solution into a high performance liquid chromatograph, adopting a reversed phase C18 chromatographic column, and taking methanol and water having a volume ratio of 35 to 65 as a mobile phase for detection. According to the method disclosed by the invention, by researching and optimizing the type, the concentration and the solid-liquid ratio of the extracting solution, the specnuezhenide in the samplecan be extracted to the greatest degree under the condition that the extracting solution is not wasted, and a foundation is laid for accurate determination. According to the method disclosed by the invention, the detection conditions of liquid chromatography are further optimized, the methanol and water having a specific ratio is selected as the mobile phase, and the detection accuracy can be effectively improved. The method for determining the specnuezhenide content through high performance liquid chromatography disclosed by the invention has the advantages of being excellent in specificity,high in sensitivity and the like.