Preparation method and application of total iridoid glycoside of ligustrum lucidum ait

Abstract

The invention relates to the technical field of medicines, and discloses a preparation method of total iridoid glycoside of ligustrum lucidum ait and application of the total iridoid glycoside of ligustrum lucidum ait to preparation of an anti-osteoporosis medicine. A ligustrum lucidum ait medicinal material is subjected to thermal extraction by using hydrous ethanol to obtain an extracting solution; the extracting solution is purified by adopting macroporous adsorption resin to obtain a total iridoid glycoside extract of the ligustrum lucidum ait. The total iridoid glycoside of the ligustrumlucidum ait contains 19.67 weight percent of specnuezhenide, 0.95 weight percent of oleuropein, 36.44 weight percent of nuezhenoside and 9.01 weight percent of oleonuezhenide. The extract can promoteformation of osteocytes and suppress bone absorption of the osteocytes; under the action of multiple relevant indexes for bone metabolism, the anti-osteoporosis effect is enhanced; the aim of preventing and treating osteoporosis is fulfilled; the total iridoid glycoside of the ligustrum lucidum ait can be used for preparing a medicine for preventing and treating the osteoporosis.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to a preparation method and application of Ligustrum lucidum total iridoid glycosides extract with anti-osteoporosis effect. Background technique [0002] Ligustrum lucidum is a plant of Oleaceae Ligustrum lucidum The dried and ripe fruit of Ait. has the effect of nourishing the liver and kidney, improving eyesight and blackening hair. It is used for liver and kidney yin deficiency, dizziness and tinnitus, soreness of waist and knees, premature graying of beard and hair, dark eyes, internal heat and quenching thirst, bone steaming and hot flashes, etc. symptom. Chemical and pharmacological studies have shown that Ligustrum lucidum mainly contains triterpenes, iridoids, phenylethyl alcohols and flavonoids, which have pharmacological effects such as liver protection, anti-tumor, hypoglycemic and anti-oxidation. It has been reported that the ethanol extract of Ligustrum lucidum ca...

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Problems solved by technology

It has been reported that the ethanol extract of Ligustrum lucidum can effectively inhibit the weight gain of rats caused by ovariectomy, increase S-Ca, S-P, reduce U-Ca, U-P, reduce the content of ALP, BALP, OCN, DPD, increase BMD of femur, tibia, and 4th vertebra in rats, and long-term medication has no obvious stimulating effect on the uterus (Cheng Min, Wang Qingwei, Liu Xueying, et al. Experimental study of Ligustrum lucidum treating osteoporosis in ovariectomized rats. Chinese Pharmacology Bulletin , 2013, 29 (2) 229-233), Ligustrum lucidum contains a variety of iridoids, among which privetin, privetin G13, oleuropein and other components can promote the formation and differentiation of osteoblasts (Huang YX, Wu YB, Wu JG, et al. Chemical Constituents from Ligustrum lucidum Differentially Promote BoneFormation and Prevent Oxidative Damage in Osteoplastic UMR-106 cells. Latin American Journal of Pharmacy, 2014, 32 (2): 258-265), but so far no privet iridoid glycoside composition has been used to prevent and treat bone loose coverage

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