186 results about "Oleuropein" patented technology

Compositions comprising hydroxytyrosol-containing formulations and treatment regiments comprising hydroxytyrosol and/or oleuropein and chemotherapeutic agents are disclosed. Compositions and/or regiments may optionally include the administration of vitamins, minerals, and anti-oxidants. Methods for using these compositions and treatment regimens for treating subjects for diseases, such as a malignancy, and for inducing or enhancing angiogenesis, treating or preventing oxidative stress, for treating or preventing high glucose-induced dysfunction, treating or preventing chemotherapy-induced dysfunction, and for improving cell viability are provided. Various methods for use of the hydroxytyrosol compositions for inhibition of lysine specific demethylase 1 (LSD1) in various cancers are also provided.

The present invention relates to methods and pharmaceutical compositions for treating obesity or obesity-related disorders in a subject suffering from or predisposed to developing obesity or an obesity-related disorder, or for inhibiting the infectivity of HIV, by administering oleuropein, an analogue or derivative thereof, or the major metabolites of oleuropein including oleuropein aglycone, hydroxytyrosol, and elenolic acid or their analogues, or derivatives thereof, an iridoid glycoside, or a secoiridoid glycoside or analogues or derivatives thereof, or any combination of the foregoing including olive leave extract. The invention also relates to methods for screening/diagnosing a subject having, or predisposed to having obesity or a related disorder by measuring the expression profiles of an adipogenic gene selected from PPARγ2, LPL and αP2 gene and gene product, or other adipogenic, lipogenic, or lipolytic genes and gene products in an individual. The invention further provides for screening for novel oleuropein analogues.

The present invention provides economical methods for collecting oleuropein aglycon from olive vegetation water, a routine byproduct in the manufacture of olive oil. The methods have the advantage of facilitating the collection of other valuable constituents of olive vegetation water, and furthermore render the olive vegetation water environmentally benign, and thus suitable for routine disposal.

The invention discloses a process method for synchronously preparing oleuropein and olive total flavonoids from olive leaves. Olive leaves serve as raw materials, and the method comprises the following steps of: performing reflux or multiple extraction, series adsorption through macroporous adsorption resin, and gradient desorption; and recovering an organic solvent in desorption solution, and performing spray drying to respectively obtain high-content extracts of the oleuropein and the olive total flavonoids. The invention has the characteristics that: the high-content extracts of the oleuropein and the olive total flavonoids can be synchronously prepared, the production cost is low, the method is environmentally-friendly, and the process is simple.

The invention provides olive-derived vegetation water substantially free of monophenolic compounds (e.g., tyrosol and its derivatives) from olive pits. According to one aspect of the invention, the pits or seeds are removed from the olives prior to pressing. The pitless pulp or meat is then pressed to obtain a liquid-phase mixture including olive oil, vegetation water, and solid by-products. The vegetation water is separated from the rest of the liquid-phase mixture and collected. The vegetation water is useful as a source of oleuropein.

The invention discloses a preparation method of an olive leaf extract and the olive leaf extract prepared by the preparation method. An olive leaf extract product of which the oleuropein content is greater than 90% is obtained through the processes of infiltration, enzymatic extraction, separation and drying on olive leaves. The whole method only comprises four steps, the adopted reagents are common non-toxic ingredients, the yield is high, the cost is low, the obtained product can be directly used for preparing medicines, health products and foods, and the method is very suitable for industrial production.

Beneficial topical compositions for treating scar or aging skin are provided. In one embodiment, the compositions include Bulbine frutescens, Centella asiatica and a phenol derived from olives, such as oleuropein. The composition may be used in the treatment of scars formed during surgery. The composition may also be used cosmeceutically in the treatment of aged skin, and may include phosphatidylserine, vitamins, and other beneficial anti-aging ingredients.

A composition containing EPA and EHA, preferably derived from fish oil, in combination with at least one of oleuropein, allicin, and policosanol having anti-inflammatory, anti-thrombotic, immunomodulatory activity as well as activity to lower triglycerides and low density lipoprotein, to increase high density lipoprotein, and to lower both systolic and diastolic blood pressure.

The invention discloses a method for extracting oleuropein from canarium album raeusch fruits by utilizing a subcritical water extraction technology, and belongs to the technical field of separation of active ingredients from natural products. The method comprises the following steps of: performing extraction by taking pre-treated canarium album raeusch fruit flesh as a raw material and taking water as a solvent under the extraction pressure of 5 to 10 MPa and the extraction temperature of 200 to 270 DEG C for 20 to 60 minutes, performing cooling, filtration and concentration, performing separation and purification by using a macroporous resin chromatographic column, and performing vacuum concentration and freeze-drying on eluent to obtain an oleuropein product with the phenol content of over 85 percent. According to the extraction method, the use of an organic solvent is avoided; the method is clean, environment-friendly, simple in equipment, easy to operate, high in extraction speed and high in efficiency, and has broad industrial application prospect; and the product is natural and nontoxic.

The present invention provides a nutritional composition and a pharmaceutical composition to be used for humans or animals comprising as active compound the oleuropein compound or one derivative thereof.

The invention discloses a method for purifying hydroxytyrosol. The method for purifying the hydroxytyrosol comprises the following steps of: dissolving an oleuropein crude product by using deionized water under stirring; passing through an adsorption type macroporous resin chromatographic column, washing with the deionized water, performing elution by using ethanol aqueous solution, and collecting eluent; performing vacuum concentration until no alcohol exists to obtain refined oleuropein aqueous solution; adding an inorganic acid to react; adding sodium hydroxide to adjust the pH value to be 7 to obtain hydroxytyrosol-containing aqueous solution; performing vacuum concentration; performing extraction by using equivalent ethyl acetate; and laminating ester and concentrating to remove the ethyl acetate to obtain faint yellow paste hydroxytyrosol with purity of more than or equal to 80 percent. In the method, the characteristic of one-way conversion from the oleuropein in an olive leaf resource to the hydroxytyrosol is completely utilized, so that the conversion rate is high, the current situation that the oleuropein component is blindly separated in the purification process of the oleuropein is changed, the oleuropein is converted into the hydroxytyrosol with high economic value, economic benefit is improved, and difficulty in preparation of high-content hydroxytyrosol is greatly reduced.

Beneficial topical compositions for treating scar or aging skin are provided. In one embodiment, the compositions include Bulbine frutescens, Centella asiatica and a phenol derived from olives, such as oleuropein. The composition may be used in the treatment of scars formed during surgery. The composition may also be used cosmeceutically in the treatment of aged skin, and may include phosphatidylserine, vitamins, and other beneficial anti-aging ingredients.

The invention discloses a preparation method of long-circulation lipidosome of an olive polyphenol extract rich in hydroxytyrosol and verbascoside. Olive leaf is taken as a raw material, and is prepared into an olive polyphenol extract by extracting with 30 to 50 percent of ethanol, separating with a ceramic membrane and an ultrafiltration membrane and concentrating with a nanofiltration membrane; 90 percent of the hydroxytyrosol, 10 to 40 percent of an oleuropein extract and 90 percent of the verbascoside are compounded to prepare the olive polyphenol extract rich in the hydroxytyrosol and the verbascoside; the hydroxytyrosol and verbascoside polyphenol as model materials, lecithin, cholesterol, a Tween-80 surfactant and the like are prepared into the corresponding lipidosome. The lipidosome has the advantages that the bioavailability of the olive polyphenol extract is remarkably increased, and high encapsulation efficiency, uniform particle size distribution, good stability, slow release property, oxidation resistance and an antibacterial effect are achieved. The preparation method is simple and feasible, equipment is simple, and the application of the lipidosome in the medicine field can be widened.

The invention discloses a natural cleanser. The natural cleanser comprises raw materials in parts by weight as follows: 5-10 parts of a goldthread extract, 5-20 parts of a coconut oil extract, 5-15 parts of an ephedra extract, 3-7 parts of a liquorice root extract, 0.5-1 part of a ginger extraction solution, 0.2-1 part of an aloe extraction solution, 0.4-1 part of oleuropein, 3-5 parts of a lemon enzyme extract and the balance of purified water. The invention further provides a preparation technology of the natural cleanser. The cleanser is environment-friendly, adopts natural and non-toxic raw materials, contains no petrochemical raw materials, phosphorus, sulfur, arsenic and other heavy metals, belongs to a non-toxic and non-irritating product and can be directly degraded into harmless substances in the nature, and a waste liquid after cleaning meets the environmental protection requirement and can be directly discharged; the natural cleanser is wide in application range, high in dirt removal capability and safe and reliable to use, has sterilizing and antibacterial functions, has the effect of protecting skin and cannot endanger the human health.

The present invention relates to use of a composition comprising oleuropein and/or hydroxytyrosol for maintenance joint health or prevention or treatment of joint disorders. In particular, the invention relates to oleuropein for use to prevent or treat cartilage breakdown.

The invention relates to a method of preparing a wide range of hydroxytyrosol esters. According to the invention, the compounds are synthesised by reacting synthetic hydroxytyrosol or natural hydroxytyrosol compounds, oleuropein or oleuropein aglycones (which are found in olive oil, vegetable water, olive marc or olive leaves) with an acylating agent which is a compound containing at least one acylic group R, wherein R is H, an alkyl radical with between 1 and 31 linear, branched or cyclic carbon atoms, an alkenyl radical with up to 31 linear, branched or cyclic carbon atoms or an aryl group. The invention also relates to the esters prepared with the inventive method, which can be used as an additive in food and cosmetic products as well as in pharmaceutical preparations.

The invention relates to a method for extracting oleuropein from olive leaves. The method comprises the following steps of: smashing olive leaves; adding saturated lime water in an amount of 8-15 times that of the olive leaves, and heating and extracting for 2-3 times; adjusting an extracting solution to be neutral; adding a filtered extracting solution into a macroporous resin column for adsorbing; gradually eluting with ethanol water; collecting active ingredients; concentrating under reduced pressure; adding water-saturated n-butyl alcohol into a concentrated solution for extracting for 3-5 times; collecting a n-butyl alcohol layer; recovering an organic solvent under reduced pressure to obtain an extract; adding ethanol for refluxing and dissolving; adding active carbon for decoloring for 1-2 hours; filtering the active carbon out; recovering ethanol; and performing freeze drying to obtain the oleuropein product. The method has the advantages of easy operating, no use of toxic reagents in a preparation process, environmental friendliness and high product content, and is suitable for industrial production.