68 results about "Oleuropein" patented technology

The invention discloses a composition for improving the stability of oleuropein in a solution as well as a preparation method and application of the composition. The composition comprises oleuropein and at least one stabilizer, and the stabilizer is selected from the group consisting of anisic acid, lactobionic acid, glycyrrhizic acid, 3, 3-thiodipropionic acid, N-acetyl-L-glutamine, propyl-cysteine and lauramidopropyl betaine; and the pH value of the aqueous solution of the composition is 4.0-8.0. The preparation method comprises the following steps: dissolving oleuropein in water, adding the stabilizer, and uniformly mixing. Through simple physical mixing, by utilizing the synergistic effect of the specific stabilizer and the oleuropein, the degradation and discoloration of the oleuropein in the storage process are remarkably inhibited. Experiments show that after the preferable composition is preserved for 30 days in an open manner, the retention rate of the oleuropein still reaches up to 80% or above, and is far superior to that of blank control and a traditional liposome wrapping method. The composition is simple and convenient in preparation process and low in cost, and provides reliable technical support for wide application of oleuropein in liquid products such as food, medicine and cosmetics.

The invention discloses a composition based on centella asiatica extract and olive extract and application of the composition in mask cloth, and relates to the technical field of cosmetics. Comprising the following raw materials: modified olive dispersion liquid, caprylic capric triglyceride, nano titanium dioxide dispersion liquid, chitosan quaternary ammonium salt, hyaluronic acid, modified centella asiatica extract, centella asiatica nanocrystals, silk fibroin composite liquid, epsilon-polylysine and deionized water. The prepared composition based on the centella asiatica extract and the olive extract effectively improves the transdermal absorption rate and stability of active ingredients such as asiaticoside and oleuropein in the extract, and can greatly improve the tensile strength, moisturizing time and skin adhesion of mask cloth when being applied to the mask cloth. Therefore, the composition based on the centella asiatica extract and the olive extract prepared by the invention has a wider application prospect.

The present invention relates to a drug carrier system in the form of nanocapsules and nanospheres loaded with luteolin and oleuropein, which have a counteracting effect on tumour cell growth, invasion and angiogenesis, and to a production method (100) of this drug carrier system.

The invention provides a wool fabric anti-pilling finishing agent and a preparation method, and relates to the field of fabric finishing agents, the wool fabric anti-pilling finishing agent comprises a dry material and a wet material, the dry material is obtained by adding a seaweed extract and oleuropein into a mixture of sericin and water and performing microwave treatment, low-temperature drying treatment and crushing treatment on the mixture, and the wet material is obtained by adding the seaweed extract and the oleuropein into the mixture. The wet material is obtained by mixing tea polyphenol, oleanolic acid and water. The anti-pilling finishing agent for the wool fabric, provided by the invention, is prepared from the sericin, the seaweed extract, the oleuropein, the tea polyphenol and the oleanolic acid materials, and the application of the natural materials not only is environment-friendly, but also can enhance the mechanical properties of the wool fabric while improving the anti-pilling property and the antistatic property of the wool fabric; after the anti-pilling finishing agent for the wool fabric is applied to the wool fabric, the wool fabric has the anti-pilling property and the durability.

The application provides a kind of dynamic monitoring method and system of olive leaf polyphenol components based on principal component analysis, it is related to computer technology field, comprising: obtaining original data;According to the original data, the spectrum interval is separated, and the purified polyphenol characteristic spectrum sequence is obtained;According to the purified polyphenol characteristic spectrum sequence, spectral deconvolution processing is carried out, and the polyphenol subcomponent concentration evolution matrix is obtained;According to the polyphenol subcomponent concentration evolution matrix, the principal component is reconstructed, and the reconstruction result is obtained;According to the reconstruction result, track fitting is carried out, by fitting the principal component score track into the kinetic network model containing hydroxytyrosol glycoside hydrolysis and oxidative condensation step, the fitting result is obtained;According to the fitting result, prediction is carried out, the evolution end point of hydroxytyrosol free state content and its derivative relative proportion is predicted, and the dynamic monitoring result is obtained.The application realizes the accurate monitoring and prediction of the dynamic conversion process of specific polyphenol components such as hydroxytyrosol glycoside and oleuropein in olive leaves.

The present invention relates to the use of a composition comprising an effective amount of oleuropein and / or a metabolite thereof in combination with taurine and / or a functional derivative for maintaining joint health in a subject or preventing or treating joint disorders in a subject. In particular, the present invention relates to a composition comprising an effective amount of oleuropein and / or a metabolite thereof in combination with taurine and / or a functional derivative for use in the prevention or treatment of cartilage degeneration in a subject.

Methods of stabilizing acidic cannabinoids by intercalating the acidic cannabinoids into layered double hydroxides (LFHs) are provided. Also provided are compositions comprising the LDH-intercalated acidic cannabinoids, physiologically compatible formulations comprising the compositions and methods of using the formulations to treat a variety of diseases and conditions in subjects.

The invention relates to the technical field of nano-drug delivery systems, in particular to an oleuropein-loaded core-shell structure drug delivery system as well as a preparation method and application thereof. The invention relates to an oleuropein-loaded core-shell structure drug delivery system, which comprises an inner core and an outer shell, the inner core is an oleuropein nanoparticle formed by self-assembly of oleuropein; the oleuropein nanoparticles are cross-linked and modified with sodium tripolyphosphate and covalently connected with folic acid; the shell is formed from ferulic acid derived lignin. According to the scheme, efficient targeted delivery and collaborative treatment of the oleuropein in treatment of sepsis-related intestinal dysfunction are achieved, the technical problems that when the oleuropein is applied to treatment of sepsis, the drug targeting property is not high, the bioavailability is not ideal, and target site release cannot be achieved can be solved, and the application of the oleuropein to treatment of the sepsis is promoted. The invention opens up a new way for developing a novel efficient targeting drug delivery system, provides an innovative strategy for clinical conversion of natural products, and has ideal application and popularization values.

The invention provides oleanolic acid, and a preparation method of the oleanolic acid comprises the following steps: S1, dissolving 5g of arginic acid in 500ml of dichloromethane, adding 7g of pyridinium dichlorochromate, stirring at room temperature for 18 hours, and detecting the reaction degree by thin-layer chromatography; s2, after the reaction is completed and vacuum concentration is carried out, a sample is mixed with 200-300-mesh silica gel, the mixture is volatilized to be dry and then placed on a silica gel column, column chromatography separation is carried out, petroleum ether / ethyl acetate is selected as a mobile phase, 2.87 g of oleanolic acid is obtained, the molecular formula is C30H46O3, and the yield is 57.6%. The oleanolic acid provided by the invention can be used for preparing medicines for treating oligospermia and medicines for improving dyszoospermia. The oleanolic acid provided by the invention is a natural product or an artificially synthesized product, is low in toxicity and small in side effect, and has good effects of improving dyszoospermia and promoting fertility.

Composition comprising a combination of oleuropein or metabolite thereof and taurine or functional derivative thereof are provided. The composition may be an oral nutritional composition, for example a nutritional supplement, an oral nutritional supplement, a food product, a food for special medical purpose (FSMP). The composition can be administered to an individual in need thereof for improving a physiological state linked to metabolic fatigue in one or more cells, (ii) increasing mitochondrial energy and mitochondrial calcium uptake in one or more cells, and (iii) increasing antioxidant capacity, reducing oxidative stress and / or enhancing mitochondrial function, (iv) treating or preventing a calcium deficiency / depletion disorder in an individual. Additionally or alternatively, the method can treat or prevent a mitochondria-related disease or a condition associated with altered mitochondrial function in an individual in need thereof or at risk thereof.

Compositions for treatment or prevention of synovitis contain a therapeutically effective amount of at least one of oleuropein, rutin or curcumin and preferably contain at least oleuropin. Methods for treatment or prevention of synovitis include administering such compositions. The compositions can additionally include one or more of quercetin, hydroxytyrosol or vitamin D.

The invention provides an extraction method for increasing the oleuropein conversion rate and the hydroxytyrosol content in olive pomace, and belongs to the technical field of natural product extraction. Based on the analysis of the characteristics of the olive pomace material, firstly, the compact structure of the olive pomace is destroyed through steam explosion pretreatment, and pores are loosened; then carrying out compound enzyme enzymolysis, cooperatively hydrolyzing wrapped binding protein by adopting acid protease and hemicellulase in a combined manner, destroying a polysaccharide wrapping barrier in the pomace, and promoting rapid release of oleuropein; finally, high static pressure extraction is adopted, ultrahigh pressure is used for accelerating solvent permeation, the conversion rate of oleuropein is increased, and the content of hydroxytyrosol is comprehensively increased. The method is green and efficient, the oleuropein conversion rate can be increased to the maximum extent, and the content of hydroxytyrosol is increased; the degradation loss of hydroxytyrosol in the extraction process is reduced, and the economic benefits of the olive pomace are comprehensively improved.

The invention discloses application of elaifolin in preparation of a medicine for resisting streptococcus suis, and belongs to the field of microbial infectious diseases and medicines. The invention finds that the elaifolin can efficiently inhibit and kill a plurality of serotype streptococcus suis and obviously inhibit or remove biological membranes of the streptococcus suis. The elaifolin can be applied to preparation of anti-streptococcus suis bacteriostasis and sterilization medicines, medicines for treating streptococcus suis infection and medicines for inhibiting or removing streptococcus suis biological membranes. According to the application disclosed by the invention, new medicinal value of the elaifolin is found, and a new prevention and treatment strategy is provided for streptococcus suis drug resistance and biofilm infection; the elaifolin can efficiently act on the multi-drug-resistant streptococcus suis, and a new direction is provided for relieving antibiotic drug resistance crisis.

The invention provides an olive leaf polyphenol component dynamic monitoring method and system based on principal component analysis, and relates to the technical field of computers, and the method comprises the following steps: obtaining original data; performing spectrum interval separation according to the original data to obtain a purified polyphenol characteristic spectrum sequence; performing spectrum deconvolution processing according to the purified polyphenol characteristic spectrum sequence to obtain a polyphenol subcomponent concentration evolution matrix; performing principal component reconstruction according to the polyphenol subcomponent concentration evolution matrix to obtain a reconstruction result; performing trajectory fitting according to a reconstruction result, and fitting a principal component score trajectory into a dynamic network model containing hydroxytyrosol glycoside hydrolysis and oxidative condensation steps to obtain a fitting result; and performing prediction according to the fitting result, predicting an evolution end point of the free-state content of hydroxytyrosol and the relative proportion of the hydroxytyrosol derivative, and obtaining a dynamic monitoring result. According to the method, the dynamic conversion process of specific polyphenol components such as hydroxytyrosol glucoside and oleuropein in the olive leaves is accurately monitored and predicted.

The application belongs to the technical field of stem cells, and specifically discloses a mesenchymal stem cell-containing composition and application of the mesenchymal stem cell-containing composition in preparation of a product with an anti-inflammatory effect. The lycopene and oleuropein are added to an activation culture medium. On one hand, the lycopene and the oleuropein can protect the biological activity of the mesenchymal stem cells, improve the immune regulation function of the mesenchymal stem cells, further promote the repair of tissues, improve the proliferation capacity of the mesenchymal stem cells, and promote the generation and secretion of active factors with an anti-inflammatory effect in the mesenchymal stem cells and effectively protect the activity of the active factors. On the other hand, the synergistic effect of the lycopene and the oleuropein can more effectively reduce the tissue damage caused by oxidative stress and inflammation, and significantly improve the anti-inflammatory effect of the composition.

The invention relates to the technical field of nano-drug delivery systems, in particular to a liver-targeting, long-circulating and acid-responsive nano-drug delivery system for loading oleuropein as well as a preparation method and application of the liver-targeting, long-circulating and acid-responsive nano-drug delivery system. The nano-drug delivery system comprises a drug-loading inner core and a functionalized surface modification layer, the medicine carrying inner core is a zeolite imidazate framework loaded with oleuropein; the functional surface modification layer is a lactobionic acid-polyethylene glycol conjugate. According to the invention, by constructing a three-stage modular nano-drug delivery system of liver targeting-long circulation-acid response, precise delivery and efficient release of oleuropein at a sepsis liver injury part are realized. According to the technical scheme, the technical problems that the bioavailability of the oleuropein is low, the targeted delivery efficiency of the oleuropein at the sepsis liver injury part is not ideal and the like can be solved. According to the technical scheme, the pharmacokinetic characteristics and targeted treatment efficiency of OLE are comprehensively improved, and an efficient and safe nano-drug delivery system is provided for precise treatment of sepsis liver injury.

The invention discloses a dissolving device for oleuropein permeability test, the dissolving device comprises a dissolving tank and a sealing cover arranged at the top end of the dissolving tank, the top end of the dissolving tank is rotatably provided with an annular plate, one side of the inner wall of the annular plate is provided with a material storage pipe through a crushing cylinder, and the upper and lower parts of the interior of the material storage pipe are provided with material carrying plates; every two adjacent material carrying plates are fixedly connected through a connecting rod. The invention relates to the technical field of oleuropein production. According to the dissolving device for testing the permeability of oleuropein, a powdery solvent obtained through crushing is conveyed into the material storage pipe, the two material carrying plates are controlled to store the powdery solvent in a quantitative manner, and in the subsequent process that the material storage pipe rotates along the inner wall of the dissolving tank, the powdery solvent is gradually released by controlling the amount of the powdery solvent; therefore, multi-space-point blanking operation of the powder solvent is realized in the dissolving tank, the powder solvent is quickly and uniformly mixed with liquid, and the dissolving speed and quality of oleuropein are further improved.

A method of preventing or treating muscle fatigue from exercise and / or for resistance to muscle fatigue from exercise, the method including orally administering at least one of oleuropein or metabolite thereof to an individual before, during and / or after the exercise. A unit dosage form contains at least one of oleuropein or metabolite thereof in an amount effective for administration of the unit dosage form before, during and / or after exercise to thereby prevent or treat muscle fatigue from the exercise and / or for resistance to muscle fatigue from exercise. A method of making a composition for preventing or treating muscle fatigue from exercise and / or for resistance to muscle fatigue from exercise, the method including adding an effective amount of at least one of oleuropein or metabolite thereof to at least one ingredient selected from the group consisting of protein, carbohydrate, and fat. The exercise is preferably one or more of 1) resistance exercise, 2) anaerobic or repeated sprint-type exercise, or 3) endurance exercise.

A composition containing oleuropein and / or hydroxytyrosol can be administered to maintain joint health or prevent or treat a joint disorder, for example by preventing or treating cartilage breakdown. The composition can decrease joint pain. An elderly mammal can be the recipient of administration.

The present invention relates to use of a composition comprising an effective amount of a combination of oleuropein and / or metabolite thereof and fisetin and / or derivative for maintenance of joint health or prevention or treatment of joint disorders in an individual. In particular, the invention relates to a composition comprising an effective amount of a combination of oleuropein and / or metabolite thereof and fisetin and / or derivative for use to prevent or treat cartilage degeneration in an individual.

The breeding method for the postpartum blue crabs is characterized by comprising a microcirculation preparation for the postpartum blue crabs, and the microcirculation preparation for the postpartum blue crabs at least contains one of the following components in content: rosmarinic acid with the content of 300-400 mg / L, carnosic acid with the content of 250-350 mg / L, oleuropein with the content of 250-350 mg / L and terpene alcohol with the content of 200-300 mg / L. The microcirculation preparation for the postpartum blue crabs can remarkably improve the growth level, the nutrition transportation capacity, the waste metabolism capacity, the tissue repair capacity and the fat and energy storage capacity of the postpartum female crabs, and promote the postpartum cream increase and rejuvenation of the blue crabs.

The invention relates to the technical field of preparation of olive leaf extracts, in particular to an olive leaf extract and a preparation process and application thereof, and the process comprises the following steps: crushing dried olive leaves, mixing the crushed olive leaves with an ethanol aqueous solution, and carrying out multi-stage temperature control hot reflux extraction and solid-liquid separation to obtain a supernatant and a precipitate; loading the supernate to a resin column, washing with water and then eluting with ethanol, and drying the eluent to obtain an oleuropein crude product; blending the oleuropein crude product and the sample stirring silica gel, drying, filling into a chromatographic column, eluting by using an ethyl acetate-ethanol mixed solvent, and collecting a high-purity oleuropein fine product; sequentially carrying out ultrasonic dissolution, concentration, standing crystallization and filtration on the precipitate through medium-concentration and high-concentration ethanol to obtain a maslinic acid crude product and an oleanolic acid crude product; and respectively heating and dissolving with ethanol, cooling and crystallizing, and circularly recrystallizing to obtain a corresponding refined product. The invention aims to guarantee the extraction yield and quality of oleuropein by controlling the multi-stage temperature of heating reflux extraction of olive leaves.

The present invention discloses an active component with antioxidant, anti-glycation and anti-inflammatory effects, as well as its preparation method and application. This component includes oleuropein, diosmin, neodiosmin, rosmarinic acid, oleuropein, salvianolic acid B, luteolin-7-O-glucoside, pectolinarigenin, ligustroside G13, 6'-O-trans-cinnamoyl-8-epigingkolic acid. Its preparation method uses Zhenzhu Tiaozhi Formula, Tongmai Formula, Baoshen Formula, etc. as raw materials, ultrasonic extraction with methanol, and the extract is separated and purified by preparative liquid chromatography or dynamic axial compression column chromatography, combined with real-time on-line ultraviolet spectrophotometric detection to obtain. Research shows that the active component of the present invention has good in vitro antioxidant, anti-glycation and anti-inflammatory activities, can significantly reduce the levels of inflammatory factors TNF-α and IL-1β in LPS-induced Raw 264.7 cells, and has a protective effect on LPS-induced liver and kidney damage in C57 / BL6J mice, and is suitable for application in the preparation of drugs for preventing or treating metabolic inflammatory diseases.

The invention discloses application of oleuropein in preparation of a product for resisting skin high-temperature injury. The skin high-temperature injury resistance is realized through the following two aspects: 1) the inflammation effect caused by temperature rise is resisted; 2) promoting wound healing; experimental results show that under the condition of temperature rise, the oleuropein remarkably enhances the migration ability of skin cells and inhibits the generation of inflammatory factors IL-6 and IL-8 at the same time, so that the application field of the oleuropein is expanded, especially in the aspect of skin care.

The present application belongs to the field of chemical engineering. Disclosed is a method for separating and purifying oleuropein by means of single column chromatography. The method comprises: performing extraction, involving: placing olive leaf powder into a non-woven fabric tea bag, and soaking and extracting same to obtain an extracting solution; preparation of an extract, involving: performing suction filtration on the extracting solution under reduced pressure, then performing rotary evaporation to obtain a residual solution, and draining same until the water content is less than or equal to 5%, so as to obtain an extract; performing sample stirring, involving: dissolving the extract, then adding silica gel thereto, and performing rotary evaporation to remove a solvent; performing column packing, involving: packing the silica gel into a chromatographic column; performing elution, involving: performing gradient elution on the chromatographic column by using an eluent at a flow speed of 5-100 mL / min; performing collection, involving: collecting the chromatographic solution with an HPLC content of more than 90% to obtain a collected solution; and performing concentration. In the present application, by controlling the drying degree (water content) of the extract (the water content not exceeding 5%), the purity of oleuropein can reach 90% or above only by means of one instance of column chromatography, and the yield (oleuropein / olive leaf powder) can reach 25-32%; and a solvent for column chromatography can be recycled.

The present application relates to the technical field of nanomedicine delivery system, and particularly relates to a liver-targeting, long-circulating and acid-responsive nanomedicine delivery system loaded with oleuropein and a preparation method and application thereof. The nanomedicine delivery system comprises a drug-loaded core and a functional surface modification layer; the drug-loaded core is a zeolitic imidazolate framework loaded with oleuropein; and the functional surface modification layer is a lactobionic acid-polyethylene glycol conjugate. The present application realizes the precise delivery and efficient release of oleuropein at the site of sepsis liver injury by constructing a liver-targeting, long-circulating and acid-responsive three-level modular nanomedicine delivery system. The technical scheme can solve the technical problems of low bioavailability of oleuropein and unsatisfactory targeting delivery efficiency at the site of sepsis liver injury. The technical scheme comprehensively improves the pharmacokinetic characteristics and targeted treatment efficiency of OLE, and provides an efficient and safe nanomedicine delivery system for the precise treatment of sepsis liver injury.

The present invention relates to use of a composition comprising an effective amount of a combination of oleuropein and / or metabolite thereof and fisetin and / or derivative for use to prevent or treat bone disorders or maintenance of bone health in an individual.

The invention discloses an antioxidant composition and application thereof. The antioxidant composition comprises oleuropein and cerium oxide. The oleuropein and the cerium oxide are compounded, so that the oxidation resistance can be enhanced, and the effect of cyclically scavenging free radicals can be improved.

The present invention relates to a standardized polyphenol substance formulation, which has an anti-cancer effect; due to oxidation resistance, inflammation resistance, liver protection, heart protection, nerve protection and systemic effects, cytotoxicity can be selectively generated on tumor cells, so that the effectiveness of standard treatment is supported; and when the medicine is used according to the recommended dosage, compared with medicines with the characteristics of cancer resistance, oxidation resistance, inflammation resistance and the like in the prior art, the medicine has the minimum side effect. The formulation mentioned consists of a combination comprising at least one of the following natural substances: a plant derived curcumin, oleuropein, epigallocatechin gallate (EGCG), resveratrol, rosmarinic acid, carnosic acid and phenethyl caffeate (CAPE), apigenin, galangin, chrysin, luteolin, quercetin and pinocembrin, and a combination comprising at least one of the following natural substances: a plant derived curcumin, oleuropein, epigallocatechin gallate (EGCG), resveratrol, rosmarinic acid, carnosic acid and phenethyl caffeate (CAPE), the natural substances exist in various plants and propolis. The active polyphenol substance content in the formulations as the subject matter of the invention varies depending on the type and stage of cancer the formulations will be used to treat. Formulations as the subject matter of the invention are prepared in the form of suspensions, syrups, pouches, tablets or capsules, containing in addition to the active ingredients an appropriate proportion of excipients such as dextrose, maltose, maltodextrin or beta-cyclodextrin.

The present application relates to a kind of solvent and method for separating high purity oleuropein with high efficiency.The mixed solvent is selected from: (1) the mixed solvent consisting of n-butanol, dichloromethane, methanol and water, wherein the volume ratio of n-butanol, dichloromethane, methanol and water is 1: (5-15): (1-5): (5-15), preferably 1: (5-10): (1-2): (5-10);(2) the first solvent, the first solvent is the upper layer after the mixed solvent of (1) is left to separate;(3) the second solvent, the second solvent is the lower layer after the mixed solvent of (1) is left to separate;And (4) the combination of first solvent and second solvent.The present application also includes the method for separating oleuropein using the mixed solvent.