24 results about "Oleuropein" patented technology

The invention discloses a composition for improving the stability of oleuropein in a solution as well as a preparation method and application of the composition. The composition comprises oleuropein and at least one stabilizer, and the stabilizer is selected from the group consisting of anisic acid, lactobionic acid, glycyrrhizic acid, 3, 3-thiodipropionic acid, N-acetyl-L-glutamine, propyl-cysteine and lauramidopropyl betaine; and the pH value of the aqueous solution of the composition is 4.0-8.0. The preparation method comprises the following steps: dissolving oleuropein in water, adding the stabilizer, and uniformly mixing. Through simple physical mixing, by utilizing the synergistic effect of the specific stabilizer and the oleuropein, the degradation and discoloration of the oleuropein in the storage process are remarkably inhibited. Experiments show that after the preferable composition is preserved for 30 days in an open manner, the retention rate of the oleuropein still reaches up to 80% or above, and is far superior to that of blank control and a traditional liposome wrapping method. The composition is simple and convenient in preparation process and low in cost, and provides reliable technical support for wide application of oleuropein in liquid products such as food, medicine and cosmetics.

The application provides a kind of dynamic monitoring method and system of olive leaf polyphenol components based on principal component analysis, it is related to computer technology field, comprising: obtaining original data;According to the original data, the spectrum interval is separated, and the purified polyphenol characteristic spectrum sequence is obtained;According to the purified polyphenol characteristic spectrum sequence, spectral deconvolution processing is carried out, and the polyphenol subcomponent concentration evolution matrix is obtained;According to the polyphenol subcomponent concentration evolution matrix, the principal component is reconstructed, and the reconstruction result is obtained;According to the reconstruction result, track fitting is carried out, by fitting the principal component score track into the kinetic network model containing hydroxytyrosol glycoside hydrolysis and oxidative condensation step, the fitting result is obtained;According to the fitting result, prediction is carried out, the evolution end point of hydroxytyrosol free state content and its derivative relative proportion is predicted, and the dynamic monitoring result is obtained.The application realizes the accurate monitoring and prediction of the dynamic conversion process of specific polyphenol components such as hydroxytyrosol glycoside and oleuropein in olive leaves.

Methods of stabilizing acidic cannabinoids by intercalating the acidic cannabinoids into layered double hydroxides (LFHs) are provided. Also provided are compositions comprising the LDH-intercalated acidic cannabinoids, physiologically compatible formulations comprising the compositions and methods of using the formulations to treat a variety of diseases and conditions in subjects.

The invention relates to the technical field of nano-drug delivery systems, in particular to an oleuropein-loaded core-shell structure drug delivery system as well as a preparation method and application thereof. The invention relates to an oleuropein-loaded core-shell structure drug delivery system, which comprises an inner core and an outer shell, the inner core is an oleuropein nanoparticle formed by self-assembly of oleuropein; the oleuropein nanoparticles are cross-linked and modified with sodium tripolyphosphate and covalently connected with folic acid; the shell is formed from ferulic acid derived lignin. According to the scheme, efficient targeted delivery and collaborative treatment of the oleuropein in treatment of sepsis-related intestinal dysfunction are achieved, the technical problems that when the oleuropein is applied to treatment of sepsis, the drug targeting property is not high, the bioavailability is not ideal, and target site release cannot be achieved can be solved, and the application of the oleuropein to treatment of the sepsis is promoted. The invention opens up a new way for developing a novel efficient targeting drug delivery system, provides an innovative strategy for clinical conversion of natural products, and has ideal application and popularization values.

Composition comprising a combination of oleuropein or metabolite thereof and taurine or functional derivative thereof are provided. The composition may be an oral nutritional composition, for example a nutritional supplement, an oral nutritional supplement, a food product, a food for special medical purpose (FSMP). The composition can be administered to an individual in need thereof for improving a physiological state linked to metabolic fatigue in one or more cells, (ii) increasing mitochondrial energy and mitochondrial calcium uptake in one or more cells, and (iii) increasing antioxidant capacity, reducing oxidative stress and / or enhancing mitochondrial function, (iv) treating or preventing a calcium deficiency / depletion disorder in an individual. Additionally or alternatively, the method can treat or prevent a mitochondria-related disease or a condition associated with altered mitochondrial function in an individual in need thereof or at risk thereof.

The invention provides an extraction method for increasing the oleuropein conversion rate and the hydroxytyrosol content in olive pomace, and belongs to the technical field of natural product extraction. Based on the analysis of the characteristics of the olive pomace material, firstly, the compact structure of the olive pomace is destroyed through steam explosion pretreatment, and pores are loosened; then carrying out compound enzyme enzymolysis, cooperatively hydrolyzing wrapped binding protein by adopting acid protease and hemicellulase in a combined manner, destroying a polysaccharide wrapping barrier in the pomace, and promoting rapid release of oleuropein; finally, high static pressure extraction is adopted, ultrahigh pressure is used for accelerating solvent permeation, the conversion rate of oleuropein is increased, and the content of hydroxytyrosol is comprehensively increased. The method is green and efficient, the oleuropein conversion rate can be increased to the maximum extent, and the content of hydroxytyrosol is increased; the degradation loss of hydroxytyrosol in the extraction process is reduced, and the economic benefits of the olive pomace are comprehensively improved.

The invention provides an olive leaf polyphenol component dynamic monitoring method and system based on principal component analysis, and relates to the technical field of computers, and the method comprises the following steps: obtaining original data; performing spectrum interval separation according to the original data to obtain a purified polyphenol characteristic spectrum sequence; performing spectrum deconvolution processing according to the purified polyphenol characteristic spectrum sequence to obtain a polyphenol subcomponent concentration evolution matrix; performing principal component reconstruction according to the polyphenol subcomponent concentration evolution matrix to obtain a reconstruction result; performing trajectory fitting according to a reconstruction result, and fitting a principal component score trajectory into a dynamic network model containing hydroxytyrosol glycoside hydrolysis and oxidative condensation steps to obtain a fitting result; and performing prediction according to the fitting result, predicting an evolution end point of the free-state content of hydroxytyrosol and the relative proportion of the hydroxytyrosol derivative, and obtaining a dynamic monitoring result. According to the method, the dynamic conversion process of specific polyphenol components such as hydroxytyrosol glucoside and oleuropein in the olive leaves is accurately monitored and predicted.

The application belongs to the technical field of stem cells, and specifically discloses a mesenchymal stem cell-containing composition and application of the mesenchymal stem cell-containing composition in preparation of a product with an anti-inflammatory effect. The lycopene and oleuropein are added to an activation culture medium. On one hand, the lycopene and the oleuropein can protect the biological activity of the mesenchymal stem cells, improve the immune regulation function of the mesenchymal stem cells, further promote the repair of tissues, improve the proliferation capacity of the mesenchymal stem cells, and promote the generation and secretion of active factors with an anti-inflammatory effect in the mesenchymal stem cells and effectively protect the activity of the active factors. On the other hand, the synergistic effect of the lycopene and the oleuropein can more effectively reduce the tissue damage caused by oxidative stress and inflammation, and significantly improve the anti-inflammatory effect of the composition.

The invention relates to the technical field of preparation of olive leaf extracts, in particular to an olive leaf extract and a preparation process and application thereof, and the process comprises the following steps: crushing dried olive leaves, mixing the crushed olive leaves with an ethanol aqueous solution, and carrying out multi-stage temperature control hot reflux extraction and solid-liquid separation to obtain a supernatant and a precipitate; loading the supernate to a resin column, washing with water and then eluting with ethanol, and drying the eluent to obtain an oleuropein crude product; blending the oleuropein crude product and the sample stirring silica gel, drying, filling into a chromatographic column, eluting by using an ethyl acetate-ethanol mixed solvent, and collecting a high-purity oleuropein fine product; sequentially carrying out ultrasonic dissolution, concentration, standing crystallization and filtration on the precipitate through medium-concentration and high-concentration ethanol to obtain a maslinic acid crude product and an oleanolic acid crude product; and respectively heating and dissolving with ethanol, cooling and crystallizing, and circularly recrystallizing to obtain a corresponding refined product. The invention aims to guarantee the extraction yield and quality of oleuropein by controlling the multi-stage temperature of heating reflux extraction of olive leaves.

The present application relates to the technical field of nanomedicine delivery system, and particularly relates to a liver-targeting, long-circulating and acid-responsive nanomedicine delivery system loaded with oleuropein and a preparation method and application thereof. The nanomedicine delivery system comprises a drug-loaded core and a functional surface modification layer; the drug-loaded core is a zeolitic imidazolate framework loaded with oleuropein; and the functional surface modification layer is a lactobionic acid-polyethylene glycol conjugate. The present application realizes the precise delivery and efficient release of oleuropein at the site of sepsis liver injury by constructing a liver-targeting, long-circulating and acid-responsive three-level modular nanomedicine delivery system. The technical scheme can solve the technical problems of low bioavailability of oleuropein and unsatisfactory targeting delivery efficiency at the site of sepsis liver injury. The technical scheme comprehensively improves the pharmacokinetic characteristics and targeted treatment efficiency of OLE, and provides an efficient and safe nanomedicine delivery system for the precise treatment of sepsis liver injury.

The present application relates to a kind of solvent and method for separating high purity oleuropein with high efficiency.The mixed solvent is selected from: (1) the mixed solvent consisting of n-butanol, dichloromethane, methanol and water, wherein the volume ratio of n-butanol, dichloromethane, methanol and water is 1: (5-15): (1-5): (5-15), preferably 1: (5-10): (1-2): (5-10);(2) the first solvent, the first solvent is the upper layer after the mixed solvent of (1) is left to separate;(3) the second solvent, the second solvent is the lower layer after the mixed solvent of (1) is left to separate;And (4) the combination of first solvent and second solvent.The present application also includes the method for separating oleuropein using the mixed solvent.

The present invention relates to the use of a composition comprising an effective amount of oleuropein and / or its metabolites in combination with taurine and / or functional derivatives for the maintenance of joint health or the prevention or treatment of joint disorders in an individual. In particular, the present invention relates to a composition comprising an effective amount of oleuropein and / or its metabolites in combination with taurine and / or functional derivatives for use in the prevention or treatment of cartilage degeneration in an individual.

The present invention relates to compositions containing a mixture of three active substances, which are derived from dive extract and are hydroxytyrosol, oleuropein, and oleocanthal, said compositions being suitable for cardioprotection.

The application describes a large scale process for the in vitro production of an olive cell culture. The application further describes a composition in a form of a powder comprising olive fruit / leaf cells grown in vitro. The cell culture of olive cells manufactured in vitro in a large scale according to the process of the invention comprises high level of verbascoside with undetectable levels of tyrosol, hydroxytyrosol, and oleuropein.

The present invention relates to compositions containing a mixture of three active substances, which are derived from dive extract and are hydroxytyrosol, oleuropein, and oleocanthal, said compositions being suitable for cardioprotection.

The present invention relates to the use of a composition comprising an effective amount of oleuropein and / or its metabolites in combination with fesoterodine and / or derivatives for the prevention or treatment of bone disorders or for the maintenance of bone health in an individual.

A method for breeding blue crab after spawning, characterized in that the method comprises a blue crab post-spawning microcirculation preparation, wherein the blue crab post-spawning microcirculation preparation contains at least one of the following components and contents: rosmarinic acid with a content of 300-400 mg / L, jasmolinic acid with a content of 250-350 mg / L, oleuropein with a content of 250-350 mg / L, and terpene alcohol with a content of 200-300 mg / L. The blue crab post-spawning microcirculation preparation can significantly improve the growth level, nutrient transport capacity, metabolic waste capacity, tissue repair capacity and fat and energy storage capacity of the post-spawning female crab, and promote the weight gain and rejuvenation of the post-spawning blue crab.

A composition is provided that includes a combination of oleuropein or a metabolite thereof and taurine or a functional derivative thereof. The composition may be an oral nutritional composition, such as a dietary supplement, an oral nutritional supplement, a food product, or a food for special medical purposes (FSMP). The composition can be administered to an individual in need thereof for (ii) improving a physiological condition associated with metabolic fatigue in one or more cells, (iii) increasing mitochondrial energy and mitochondrial calcium uptake in one or more cells, and (iv) improving antioxidant capacity, reducing oxidative stress, and / or enhancing mitochondrial function, or (iv) treating or preventing calcium deficiency / depletion disorders in the individual. Additionally or alternatively, the method can be used to treat or prevent a mitochondrial-related disease or condition associated with altered mitochondrial function in an individual in need of or at risk for such disease or condition.

The present invention relates to methods and compositions comprising a combination of oleuropein and / or metabolite thereof and adenosylcobalamin in an effective amount for use in achieving at least one result selected from the group consisting of i) preventing and / or treating a mitochondria-related disease or condition associated with altered mitochondrial function and / or ii) at least one physical state selected from the group consisting of oxidative stress or a condition associated with oxidative stress in an individual; (iii) increasing mitochondrial energy, cellular energy, mitochondrial function and mitochondrial calcium uptake in one or more cells, (iv) increasing resistance to age-related pathologies; (v) improving a physiological state or disorder related to cell ageing or metabolic fatigue in one or more cells; (vi) improving mobility and / or (vii) improving healthspan and / or lifespan in an individual.

The application discloses a composition based on extract of eriocaulon and extract of olea europaea and application thereof in facial mask cloth, and relates to the technical field of cosmetics. The composition comprises the following raw materials: modified olea europaea dispersion, glyceryl caprylcaprate, nano titanium dioxide dispersion, chitosan quaternary ammonium salt, hyaluronic acid, modified eriocaulon extract, eriocaulon nanocrystal, silk fibroin composite liquid, epsilon-polylysine and deionized water. The composition based on the extract of eriocaulon and the extract of olea europaea prepared by the application effectively improves the transdermal absorption rate and stability of effective components such as eriocaulonin and oleuropein in the extract, and when applied to the facial mask cloth, can greatly improve the tensile strength, moisturizing duration and skin adhesion of the facial mask cloth. Therefore, the composition based on the extract of eriocaulon and the extract of olea europaea prepared by the application has a more extensive application prospect.

The invention provides fermented tobacco leaves and a preparation method thereof. The preparation method of the fermented tobacco leaves comprises the following steps: mixing a glossy privet fruit extract with cigar tobacco leaves to obtain a mixture; and performing fermentation treatment on the mixture to prepare the fermented tobacco leaves. Through research, the inventor finds that the ligustrum lucidum ait extract and the cigar tobacco leaves are mixed and fermented, the ligustrum lucidum ait extract and the cigar tobacco leaves are rich in ligustroside, salidroside, ligustrum lucidum ait flavonoid glycoside and oleuropein, and the maturity and sensory quality of the cigar tobacco leaves can be obviously improved.

The present invention relates to the use of a composition comprising an effective amount of oleuropein and / or its metabolites in combination with quercetin and / or derivatives for maintaining joint health or preventing or treating joint disorders in an individual. In particular, the present invention relates to a composition comprising an effective amount of oleuropein and / or its metabolites in combination with quercetin and / or derivatives for use in preventing or treating cartilage degeneration in an individual.

The present invention provides the use of oleuropein or a derivative thereof in immunotherapy, in particular as an adjuvant. In particular, the present invention provides the use of oleuropein in combination with a checkpoint inhibitor in the treatment of cancer. The invention also provides a combination comprising oleuropein and a checkpoint inhibitor, as well as a pharmaceutical composition and a kit comprising the combination. Advantageously, the oleuropein reprograms the TAMC, increasing sensitivity to checkpoint inhibitor based anti-cancer therapies.