Method for subcritical water extraction of oleuropein

A technology of olive polyphenols and subcritical water, applied in the direction of organic chemistry, etc., to achieve the effect of simple operation of equipment

Inactive Publication Date: 2012-08-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the application of subcritical wa

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Fresh olive fruit is used as raw material, washed with water, blanched in boiling water containing 0.1% citric acid for 2 to 5 minutes, cooled, crushed to remove the pit, added 1:1 water for beating, the fruit pulp is vacuum dried, and crushed for 40 minutes Mesh sieve to get olive pulp powder. Put the olive pulp powder in the extraction kettle, pump in deionized water according to the ratio of material to liquid 1:20, control the extraction pressure to 5Mpa, the extraction temperature to 200°C, and the extraction time to 20 minutes. After the extraction, open the pressure relief valve and cool to room temperature. Obtain olive polyphenol extract. The extract is concentrated by rotary evaporation, and adsorbed on a macroporous resin chromatography column. First, water is used as an eluent to remove impurities such as sugar and protein, and then 70% alcohol solution is used as an eluent to elute phenolic substances. After vacuum concentration and freeze-drying, a solid ...

Embodiment 2

[0015] Fresh olive fruit is used as raw material, washed with water, blanched in boiling water containing 0.1% citric acid for 2 to 5 minutes, cooled, crushed to remove the pit, added 1:1 water for beating, the fruit pulp is vacuum dried, and crushed for 40 minutes Mesh sieve to get olive pulp powder. Put the olive pulp powder in the extraction kettle, pump in deionized water according to the ratio of material to liquid 1:25, control the extraction pressure to 8Mpa, the extraction temperature to 230°C, and the extraction time to 30 minutes. After the extraction, open the pressure relief valve and cool to room temperature. Obtain olive polyphenol extract. The extract is concentrated by rotary evaporation, and adsorbed on a macroporous resin chromatography column. First, water is used as an eluent to remove impurities such as sugar and protein, and then 75% alcohol solution is used as an eluent to elute phenolic substances. After vacuum concentration and freeze-drying, a solid ...

Embodiment 3

[0017] Fresh olive fruit is used as raw material, washed with water, blanched in boiling water containing 0.1% citric acid for 2 to 5 minutes, cooled, crushed to remove the pit, added 1:1 water for beating, the fruit pulp is vacuum dried, and crushed for 40 minutes Mesh sieve to get olive pulp powder. Put the olive pulp powder in the extraction kettle, pump in deionized water according to the ratio of material to liquid 1:30, control the extraction pressure to 10Mpa, the extraction temperature to 250°C, and the extraction time to 60 minutes. After the extraction, open the pressure relief valve and cool to room temperature. Obtain olive polyphenol extract. The extract is concentrated by rotary evaporation, and adsorbed on a macroporous resin chromatography column. First, water is used as an eluent to remove impurities such as sugar and protein, and then 80% alcohol solution is used as an eluent to elute the phenolic substances. After vacuum concentration and freeze-drying, a s...

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PUM

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Abstract

The invention discloses a method for extracting oleuropein from canarium album raeusch fruits by utilizing a subcritical water extraction technology, and belongs to the technical field of separation of active ingredients from natural products. The method comprises the following steps of: performing extraction by taking pre-treated canarium album raeusch fruit flesh as a raw material and taking water as a solvent under the extraction pressure of 5 to 10 MPa and the extraction temperature of 200 to 270 DEG C for 20 to 60 minutes, performing cooling, filtration and concentration, performing separation and purification by using a macroporous resin chromatographic column, and performing vacuum concentration and freeze-drying on eluent to obtain an oleuropein product with the phenol content of over 85 percent. According to the extraction method, the use of an organic solvent is avoided; the method is clean, environment-friendly, simple in equipment, easy to operate, high in extraction speed and high in efficiency, and has broad industrial application prospect; and the product is natural and nontoxic.

Description

technical field [0001] The invention relates to a method for extracting olive polyphenols from olive fruit by using subcritical water extraction technology, and belongs to the technical field of natural product active ingredient separation. Background technique [0002] Olive (Canarium album Raeusch.) is a unique subtropical fruit tree in southern my country, mainly distributed in Guangdong, Fujian and other regions of China. Olive fruit is a traditional medicinal and edible resource in my country, which has high edible and medicinal value. As an important medicinal ingredient in olive fruit, olive polyphenol has a strong antioxidant function, and its positive effect on human health has attracted extensive attention from researchers in the fields of food and medicine. The development and utilization of olive polyphenol become one of the hot spots. [0003] At present, the research on the industrial development of olive polyphenols has just started, and in recent years it h...

Claims

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Application Information

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IPC IPC(8): C07G99/00
Inventor 何志勇陈洁曾茂茂秦昉
Owner JIANGNAN UNIV
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