193 results about "Verbascoside" patented technology

The invention provides a preparation method of a Cistanche tubulosa extract and a skin-whitening anti-aging cosmetic. The preparation method comprises pulverizing, performing crude extraction, centrifugally collecting the obtained crude extract, standing, eluting, and drying to obtain the Cistanche tubulosa extract. The preparation method is simple, and has no pollution. The extract has the content of echinacoside not lower than 25%, the content of verbascoside not lower than 55%, and the content of total glycosides not lower than 80%. Proven by the antioxidant activity test, the Cistanche tubulosa extract has antioxidant, skin whitening and anti-aging effects. The skin-whitening anti-aging cosmetic is prepared by adding the Cistanche tubulosa extract into a cosmetic, is safe and free of stimulation proven by cytotoxicity and safety test, and also has good skin whitening effect.

The invention relates to a preparation method of cistanche deserticola phenylethanoid glycosides. The method comprises the steps of: cistanche deserticola crushing, lixiviating, absorbent resin adsorption, elution, and concentration, etc. In research of the extraction and stability of cistanche deserticola phenylethanoid glycoside, under a weakly acidic condition with pH of 2-4, the cistanche deserticola phenylethanoid glycoside can have substantially improved stability, and under the condition, the cistanche deserticola phenylethanoid glycoside extracted from cistanche deserticola is mainly composed of echinacoside and verbascoside. And in cistanche deserticola phenylethanoid glycosides, the total content proportion of echinacoside and verbascoside is greater than 90%. The content of cistanche deserticola phenylethanoid glycosides extracted by the method of the invention is obviously higher when compared with other extraction methods. And the obtained cistanche deserticola extract has stable effective component content and relative light color. The method provided in the invention avoids decomposition and oxidation of cistanche deserticola phenylethanoid glycoside, and is in favor of product stability.

The invention belongs to the technological field of the Chinese medicine, particularly relating to an extraction and separation processes of the active ingredients with the active function of treating the chronic glomerulonephritis. The mullein indican is the active compound researched and developed for many years in our research institute; the mullein indican is extracted, separated and refined from the digitalis leaf and is an active compound with the function of treating the chronic glomerulonephritis. The invention is the research result of many years held and developed by the Chinese Medicine Research Department of the Chinese Medicine Research Institute of China and is supported by the special fund of the Central Institute of the National Science and Technology Department; now the compound has been approved by the State Food and Drug Administration Bureau as the novel drug and the mechanism research of the treatment of the chronic nephritis has been completed; the pre-clinical research work includes the main pharmacodynamic experiment, the production technology experiment (including the industrialized extraction and separation processes), the quality control standard, the experiment of the affecting factors of the quality, the experiment of the long-term stability, the acute toxicity of the rat, the experiment of the long-term toxicity, the teratogenic and mutagenic experiment, the general pharmacological experiment, the pharmacokinetics experiment, and so on. The digitalis leaf is mainly produced in the Huaiqing area of the Henan province, and is also grown wild and cultivated in the Shaanxi, Hebei and other places of China.

The invention discloses an osmanthus phenylethanoid glycoside extract. The total phenylethanoid glycoside content in the osmanthus phenylethanoid glycoside extract is 30-90 wt%; and the total phenylethanoid glycosides comprise verbascoside and isoverbascoside. The verbascoside content in the osmanthus phenylethanoid glycoside extract is 25-80 wt%, and the weight ratio of the verbascoside to the isoverbascoside in the total phenylethanoid glycosides is 48:1-300:1. The invention also discloses a preparation method of the osmanthus phenylethanoid glycoside extract and application of the osmanthus phenylethanoid glycoside extract in preparing foods, drugs or health products for preventing and treating diabetes.

The invention provides a method that effectively stops and restrains the active ingredients of desertliving cistanche from serious degrading or losing caused by enzyme catalytic reaction initiated during the processing, drying and production technology processes by adopting an improved drying processing manner of the desertliving cistanche. The method is characterized in that: microwave is used for heating and sterilizing enzyme at high temperature, polar molecules contained in the meat stalk cell of fresh desertliving cistanche that is rich in water are led to make high frequency swing, vibration and friction and extrusion between the molecules by using a microwave high frequency electric field so as to lead the temperature inside the cell of the desertliving cistanche and the surface thereof to raise rapidly, thus generating thermal effect that has the function of enzyme sterilization and decoction pieces of the desertliving cistanche is dried rapidly by adopting microwave drying technology, thus effectively preserving the content of the active ingredients of phenylethanoid glycosides, etc. that is rich in the meat stalk of the fresh desertliving cistanche and greatly promoting the quality and medical value of the prepared decoction pieces of the desertliving cistanche. Upon analysis and determination, the content of the active ingredients of the phenylethanoid glycosides, etc. contained in the prepared decoction pieces, which is in the shapes of filiform, elongated flake or granular, of the desertliving cistanche is rich, the content of the main index ingredients of echinacoside and verbascoside monomeric compounds of the prepared decoction pieces of the desertliving cistanche, which used for weighing the quality thereof, is up to 13-24.1 percent (being calculated based on dry substance) and the water content thereof is 1.2-3.8 percent.

The invention provides an olive leaf extract which comprises any one or a combination of 5%-50% by mass of hydroxytyrosol, Oleuropein and Verbascoside. The invention further provides a preparation method of the olive leaf extract and application in cosmetics. The olive leaf extract has a high anti-aging effect because the olive leaf extract can greatly increase the content of collagen in a corium layer of a human body, so that the elasticity of the skin of the human body is maintained, and wrinkles are inhibited fundamentally. In addition, the olive leaf extract can obviously inhibit generation of free radicals (DPPH) and can serve as a product or an additive to be applied to foods, medicines and skin care products and / or the cosmetics, and the double effects of resisting oxidation and aging are played.

The invention discloses a six-flavor hematinic capsule, its quality control method and an application thereof. The six-flavor hematinic capsule is prepared by adding auxiliary materials into raw materials consisting of Chinese angelica, Ligusticum wallichii, Radix Astragali, prepared rehmannia root, lithospermum and white peony root. According to the quality control method of the six-flavor hematinic preparation, whether the six-flavor hematinic capsule contains white peony root, Chinese angelica, Radix Astragali, prepared rehmannia root, lithospermum, Ligusticum wallichii and components is identified by thin layer chromatography; by high-performance liquid chromatography, in vitro dissolution behavior of the six-flavor hematinic capsule is determined, and effective component groups are identified by fingerprint; verbascoside and calycosin glucoside are used as reference substances to simultaneously determine contents in the six-flavor hematinic capsule; and the content of a volatile component ligustilide in the six-flavor hematinic capsule is determined by a gas chromatographic method. The method provided by the invention is simple to operate, is accurate and advanced, has good linear relation, reappearance, precision, stability and recovery rate, can be adopted to effectively control product quality and guarantee curative effect of the product. The invention also provides an application in the preparation of a medicine for treating eye damage and blurred vision.

The present invention relates to an extraction and separation method for echinacoside and verbascoside in cistanche. The method comprises the following steps: dicing the cistanche and drying the cistanche; adding 75% ethanol into a multi-functional ultrasonic extraction tank to extract for three times; combining extraction liquid and concentrating the extraction liquid until no ethanol exists; filtering and clarifying the extraction liquid and the concentration liquid of the cistanche by using ultrafiltration equipment; performing absorption on the liquid by using a macroporous resin column; firstly washing the liquid with water until the liquid is colorless, then eluting the liquid by using ethanol with certain concentration; collecting an elution solution that contains the echinacoside and the verbascoside; concentrating the elution solution by using an outer circulating vaccum concentrator; carrying out spray-drying on the concentration liquid to obtain the cistanche extract; and carrying out efficient liquid-phase tracing detection on the cistanche extract, wherein the echinacoside accounts for greater than or equal to 25%, and the verbascoside accounts for greater than or equal to 10%. The total content of phenylethanoid glycosides is greater than or equal to 80%, and the yield is 20%.

The invention discloses application of an osmanthus phenylethanoid glycoside extract in preparing anti-aging drugs or health products. The total phenylethanoid glycoside content in the osmanthus phenylethanoid glycoside extract is 30-90 wt%, and the total phenylethanoid glycosides comprise verbascoside and isoverbascoside. The verbascoside content in the osmanthus phenylethanoid glycoside extract is 25-80 wt%, and the weight ratio of the verbascoside to the isoverbascoside in the total phenylethanoid glycosides is 48:1-300:1.

The invention provides a production method, which can enable the content of Phenylethanoid Glycoside combination before and after the processing to keep stable through improving the processing treatment technology of a broomrape decoction piece, wherein, the Phenylethanoid Glycoside combination is the active ingredient abundant in broomrape. Through a high-frequency microwave electrical field, the invention leads a polar molecule in a succulent stem cell of a waterrich fresh broomrape to perform high-frequency swing, vibration, and intermolecular friction and extrusion to cause the inner and surface temperature of a broomrape cell to rise rapidly; the pyrometric effect for inactivating the enzyme activity, so as to effectively block and restrain the degradation loss of the Phenylethanoid Glycoside combination caused by the enzymatic reaction induced by enzyme systems such as hydrolytic ferment, glucoside hydrolase, etc., abundant in a fresh broomrape cell; at the same time, the broomrape decoction piece is dried through the technology for microwave drying or other drying methods. The analysis determination shows that the content of echinacoside and a verbascoside monomer compound which are the main index components for weighing the quality of the broomrape decoction piece in the acquired broomrape decoction piece can reach 13 percent to 24 percent (by dry matters), and the water content is 1.3 percent to 4.1 percent.

The invention discloses a preparation method of long-circulation lipidosome of an olive polyphenol extract rich in hydroxytyrosol and verbascoside. Olive leaf is taken as a raw material, and is prepared into an olive polyphenol extract by extracting with 30 to 50 percent of ethanol, separating with a ceramic membrane and an ultrafiltration membrane and concentrating with a nanofiltration membrane; 90 percent of the hydroxytyrosol, 10 to 40 percent of an oleuropein extract and 90 percent of the verbascoside are compounded to prepare the olive polyphenol extract rich in the hydroxytyrosol and the verbascoside; the hydroxytyrosol and verbascoside polyphenol as model materials, lecithin, cholesterol, a Tween-80 surfactant and the like are prepared into the corresponding lipidosome. The lipidosome has the advantages that the bioavailability of the olive polyphenol extract is remarkably increased, and high encapsulation efficiency, uniform particle size distribution, good stability, slow release property, oxidation resistance and an antibacterial effect are achieved. The preparation method is simple and feasible, equipment is simple, and the application of the lipidosome in the medicine field can be widened.

The invention provides a system integration technology through cell disruption, slurry extraction, membrane separation and macroporous resin adsorption and chromatography separation and purification, high temperature enzyme deactivation and spray drying and the like, which achieves a technical process that from the time fresh cistanche raw materials are fed until obtaining active matters such as monomeric compounds which are rich in echinacoside and verbascoside and the like in 30 to 80 minutes, and the invention effectively overcomes a technical defect that active components such as benzyl carbinol glycosides compounds are normally seriously degraded and lost, which are caused by an enzymatic reaction which is initiated by an imperfect dry treatment method of cistanche, and the invention obviously improves the medical value of cistanche. In the active maters of cistanche which are obtained ( calculating by dry matter), the productivity of echinacoside monomeric compound reaches to 9.8% to 19.6%, and the yield of verbascoside monomeric compound reaches to 2.0% to 7.5%. The content of echinacoside monomeric compound in active matters of cistanche which are obtained reaches to 15% to 49.6%, and the content of verbascoside monomeric compound reaches to 5% to 26.7%.

The invention discloses a composition with an anti-glycosylation effect and an application thereof. The anti-glycosylation composition consists of the following components in parts by mass: 0.1 to 5 parts of an osmanthus fragrans flower extract, 0.1 to 5 parts of a pomegranate extract and 0.01 to 0.1 part of a canarium album fruit extract, wherein the mass content of polyphenol in the osmanthus fragrans flower extract is greater than or equal to 10 percent, the mass content of verbascoside in the osmanthus fragrans flower extract is greater than or equal to 10 percent, the mass content of polyphenol in the pomegranate extract is greater than or equal to 30%, the mass content of punicalagin in the pomegranate extract is greater than or equal to 8%, wherein the mass content of polyphenol inthe canarium album fruit extract is greater than or equal to 10%, and the mass content of hydroxytyrosol in the canarium album fruit extract is greater than or equal to 3%. Through the synergistic effect of the osmanthus fragrans flower extract, the pomegranate extract and thecanarium album fruit extract which have a specific proportion and specific quality, anti-glycation capacity of skins is improved in three aspects that generation of a fibroblast glycosylation reaction end product CML is remarkably reduced, generation of AGEs is reduced, and SOD expression is increased.

The invention relates to a process for preparing verbascoside. In the process, plantain seed, butterflybush flower or pedicularisL plants serving as raw materials are crushed and soaked in alkaline solution for extraction for 1.5 to 3 hours; and the extract is decolorized and is separated and purified by a resin column, the purified extract is concentrated, and the concentrate is dried to obtain the product. The process, when used for producing the product, has the advantages of simple operation, repeated use of equipment, low operation cost, high product quality and the like.

The invention provides a cistanche deserticola medicinal material processing method including the following steps: 1) impurities in a fresh Cistanche deserticola medicinal material are removed, sediment is rapidly flushed away with water, the fresh Cistanche deserticola medicinal material is cut into thick slices; 2) high temperature enzyme killing process of the thick slices obtained in step 1 is performed for 1 and 15min; 3) after heat treatment the thick slices are lyophilized. Cistanche deserticola decoction pieces processed by the method are white in color, beautiful in appearance and dry in texture, and the content of main effective constituents of echinacoside and verbascoside is 10 times greater than that of a natural drying method.

The present invention relates to the technical field of health-care foods, and relates to a composition containing herba cistanches and herba epimedii extracts, and a preparation method and an application thereof. The composition containing the herba cistanches and herba epimedii extracts is prepared from the following raw materials in parts by weight: the herba cistanches extract, the herba epimedii extract, a nutritional supplement, microcrystalline celluloses or mannitol, magnesium stearate and a flavoring agent. Compared with echinacoside and verbascoside total amount and the herba epimedii content in the existing health-care products, those of the composition containing the herba cistanches and herba epimedii extracts are greatly improved. Besides, compared with a traditional Chinese medicine of America ginsengs, the composition containing the herba cistanches and herba epimedii extracts has better effects in relieving body fatigues. At the same time, the composition containing the herba cistanches and herba epimedii extracts has better improvements in blur vision, frequent yawns, dizziness, shoulder stiffness, slow response and attention decline caused by driving fatigues.

The invention discloses a process for processing prepared rehmannia root. The method includes: firstly steaming raw rehmannia root with saturated steam and collecting the generated rehmannia root juice, performing cooling to 50-65DEG C, extruding the rehmannia root, then introducing saturated steam to conducting steaming and collecting rehmannia root juice; and drying the well steamed prepared rehmannia root for 8-10h, then adding the rehmannia root juice to conduct stewing for 10-16h, and then further performing drying for 40-50h. The prepared rehmannia root processed by the method provided by the invention has a verbascoside content up to 0.040-0.057%, which is significantly higher than the requirement of 0.02% in 2010 edition ''Pharmacopoeia of the people's republic China'', and the processed prepared rehmannia root has stable quality and high qualified rate.

The invention relates to the field of natural medicines, in particular to a Chinese herbal compound composition for reducing blood glucose. The Chinese herbal compound composition is prepared from thefollowing active pharmaceutical ingredients in weight amount: 5 to 10mg / g of cortex cinnamomic general flavone, 6 to 11mg / g of cortex cinnamomi polyphenol, 0.5 to 1.0mg / g of procyanidine, 10 to 16mg / g of radix rehmanniae preparata polysaccharide, 0.6 to 1.5mg / g of rehmannia glutinosa libosch A, 0.6 to 1.6mg / g of rehmannia glutinosa libosch D, 0.5 to 1.5mg / g of verbascoside, and 0.4 to 7.2mg / g ofcatalpol. The traditional Chinese medicine composition for reducing blood glucose provided by the invention is stable in product, has the effects on increasing insulin sensitivity, promoting insulin secretion and reducing blood glucose, and meanwhile, has the effects on regulating blood lipids and improving a liver function. The composition provided by the invention is widely applied to medicinesor health care products for treating and preventing diabetes, diabetic complication and regulating blood lipids.

The invention relates to the field of natural drug and specifically relates to a callicarpa nudiflora extract containing phenylethanoid glycoside and a preparation method thereof. The preparation method comprises the following steps of: heating callicarpa nudiflora leaves with water, carrying out reflux extraction, filtering the extracting solution, concentrating, adding a clarifying agent for clarifying the concentrated solution, enabling the clarified solution to pass through a macroporous resin column, firstly eluting with pure water, then, eluting with alcohol with low-concentration alcohol, finally, eluting with high-concentration alcohol, collecting the high-concentration alcohol eluant and drying, thus obtaining the callicarpa nudiflora extract. In the callicarpa nudiflora extract provided by the invention, total phenylethanoid glycoside content is up to 60-80%, and the sum of contents of three constituents including forsythiaside B, verbascoside and isoacteoside is up to 50%-70%.

The invention belongs to the field of traditional Chinese medicine extraction, and especially relates to a preparation method of high purity cistanche phenyl ethanol glycosides and an application of high purity cistanche phenyl ethanol glycosides in the preparation of drugs for treating pulmonary fibrosis. The preparation method extracts two high purity cistanche phenyl ethanol glycosides from cistanche. The invention specifically discloses a preparation method and applications of high purity cistanche phenyl ethanol glycosides. Three resin columns are used together; Amberlite XAD16HP macroporous resin is used to remove impurities such as sugar; Amberlite FPC22Na (macroporous strongly acidic styrene resin) and D201 (strong alkali I type anion exchange resin) are used to effectively removepigments and components that are not phenyl ethanol glycosides; three resins are screened from a plurality of resins, the extracted cistanche phenyl ethanol glycosides have a light yellow color; the content of total phenyl ethanol glycosides is more than 95% (weight ratio), the content of echinacoside and verbascoside is more than 85% (weight ratio), and the phenyl ethanol extract has a good effect on preventing pulmonary fibrosis.

The invention relates to a cultivation method for improving the yield and the quality of Cistanche deserticola. Annual Haloxylon ammodendron saplings are selected, and are planted in desertified loess in spring or autumn according to a planting density of 0.15-0.52 plant / m<2>, humus soil is applied according to a ratio of 1000-2000 g / plant, the planted samplings are timely watered to ensure the survival rate, inoculation with a Cistanche deserticola seed mixture is carried out after the Haloxylon ammodendro saplings grow to about 1.5 m, the humus soil is applied again according to a ratio of 500-2000 g / plant, the inoculation time is March to May Or October to December every year, the Cistanche deserticola seed mixture comprises Cistanche deserticola seeds subjected to seed soaking treatment in a seed soaking solution, sucrose, L-tyrosine, L-phenylalanine and mint powder, the seed soaking solution is a 3-5 mg / L fluridone solution, the seed soaking treatment time is 12-36 h, and the Cistanche deserticola seeds subjected to seed soaking treatment are obtained through drying the soaked seeds in the shade. The cultivation method has the following advantages: the inoculation process of the Cistanche deserticola seeds is simple, and can effectively promote the germination of the Cistanche deserticola seeds and effectively improve the yield of the Cistanche deserticola planted in the desertified loess; and the composition of the Cistanche deserticola seed mixture can be screened to obviously improve the content of effective components comprising echinacoside and verbascoside in the artificially planted Cistanche deserticola, improve the quality of the artificially planted Cistanche deserticola and effectively enhance the drug effects and the economic benefits of the artificially planted Cistanche deserticola.

The invention provides a method for preparing prepared rehmannia root decoction pieces. The method comprises the steps of selection, grading, cleaning, steaming, pre-drying, slicing, medicine moistening and packaging. The medicine moistening means moistening rehmannia root by using medicine juice steamed in the steaming step. The prepared rehmannia root decoction pieces prepared according to the preparation method has an extractum content of 72-80%, the content of rehmannia root reducing sugar is 35.2-36.7%, the content of polysaccharides is 3.5-3.8%, the content of verbascoside (C29H36O15) is 0.034-0.038%, the quality is high, the quality standard of the prepared rehmannia root decoction pieces is met, and the preparation method is simple, saves time and energy and is suitable for industrial production.

The invention discloses a method for improving the content of verbascoside in rehmannia by virtue of transformation of RgPAL1 gene, and belongs to the field of biotechnology. The method comprises the following steps: cloning gene RgPAL1 from rehmannia, constructing a plant expression vector containing the RgPAL1, transforming the RgPAL1 into the rehmannia by virtue of mediation of agrobacterium tumefaciens and promoting regeneration of a plant, detecting integration of an exogenous target gene RgPAL1 through polymerase chain reaction (PCR), quantitatively analyzing the content of verbascoside in the transgenic rehmannia by virtue of HPLC (High Performance Liquid Chromatoraphy)-PDA, and screening to obtain a transgenic rehmannia plant having improved verbascoside content. By virtue of the method disclosed by the invention, the content of verbascoside in the transgenic rehmannia is significantly improved, and the content of verbascoside in the RgPAL1 transgenic rehmannia is 2.57 times as much as that in non-transgenic rehmannia, so that a feasible method is provided for scaled production of verbascoside from the transgenic rehmannia. The method disclosed by the invention provides an innovative and practical related method and technology for improving the verbascoside content.

A method for measuring contents of 14 chemical components in a traditional Chinese medicine composition is disclosed. HPLC-MS / MS is adopted to simultaneously measure the contents of the 14 chemical components in the composition. The composition is a pulse-invigorating heart-tonifying pill. The 14 chemical components include emodin, lobetyolin, 2,3,5,4'-tetrahydroxy stilbene-2-O-beta-D-glucoside, glycyrrhizic acid, liquiritin, liquiritigenin, polydatin, rutoside, isoliquiritigenin, ononin, verbascoside, gallic acid, schizandrin, and rhein. The method includes building standard curves of the 14chemical components; acquiring multi-reaction ion chromatograms of the 14 chemical components in the pill; and determining the contents of the 14 chemical components in the pill. The method has characteristics of high sensitivity, high reliability, a high efficiency and capability of being rapid. Through measuring the contents of the 14 chemical components in the pill, quality control on the pillcan be more effectively performed, thus ensuring clinical treatment effects of the pill.

The invention discloses a fresh cistanche decoction piece and a preparation method thereof. The preparation method comprises fresh cistanche selection, cleaning and enzyme killing: picking and cleaning fresh cistanche, putting the cistanche into a drug moistening machine at a temperature of 86-93 DEG C and carrying out steam treatment in the drug moistening machine for 20-30min, drying: putting the cistanche into a baking box, carrying out baking at a temperature of 60 DEG C for 4-6h, transferring the cistanche into a sun-drying shed and carrying out drying in the shade, wherein in drying in the shade, the cistanche is turned twice every day, cutting, drying: carrying out drying at a temperature of 55-65 DEG C for 4-6h, and sorting and package. The preparation method directly treats fresh cistanche in a producing area so that the treated raw material cistanche has no dry head or has dry head content of 5% or less and the whole cistanche can be used as decoction pieces. An experiment proves that compared with the prior art, the preparation method improves verbascoside and echinacoside content by 2-5 times and improves polysaccharide content.

The invention discloses a verbascoside biosynthesis pathway and verbascoside biosynthesis enzyme related genes. Tuberous roots of rehmannia at six different development stages serve as materials, overall RNA (ribonucleic acid) is extracted and detected, combined transcriptome sequencing is performed by an Illumina HiSeq2500 platform to obtain 149.8 million reads, and head assembly is performed to obtain 96961 Unigenes sequences. The Unigenes sequences are compared with a KEGG (Kyoto encyclopedia of genes and genomes) database to perform metabolism pathway analysis and obtain 280 KEGG pathways, a metabolism pathway is compared with a supposed verbascoside biosynthesis pathway, and 5 candidate KEGG pathways are screened. The supposed verbascoside biosynthesis pathway is compared with the screened 5 candidate KEGG pathways, 19 gene coding enzyme catalysis verbascoside biosynthesis pathways are built, the Unigenes sequences and the database are subjected to Blast X comparison, and ESTscan software functions are added to obtain 13 candidate verbascoside biosynthesis enzyme related genes. A foundation is laid for illuminating the complete verbascoside biosynthesis pathway and producing verbascoside by secondary metabolism engineering or synthetic biology.

The invention discloses a method for producing verbascoside by using hairy roots of rehmannia, and belongs to the technical field of bio-culture. The method comprises the following step: putting the hairy roots of the rehmannia into a salicylic acid-containing culture solution for inducing culture to obtain the verbascoside. The theory of the method is that the hairy roots, which are treated by salicylic acid, of the rehmannia can enhance the expression of key biosynthetic enzymes, such as phenylalnine ammonialyase (PAL), 4-coumaroyl-co-A ligase (4CL), cinnamate 4-hydroxylase (C4H), coumaric acid hydroxylase (C3H), copper amine oxidase (CuAO) and tyrosine carboxy-lyase (TyDC), for the verbascoside within a short time period (12 to 24 h) so as to complete gathering of the verbascoside and further increase the yield of the verbascoside. The method is easy and convenient to operate, short in culture period, convenient for industrial, large-scale and standardized implementation, and has a great significance for promotion of large-scale production of the verbascoside.

The invention discloses a method for measuring the content of verbascoside in a callicarpa nudiflora preparation, relating to a method for measuring the content of effective ingredients in the callicarpa nudiflora preparation. A high performance liquid chromatography (HPLC) is used for measuring the content of verbascoside in the test article solution of the callicarpa nudiflora preparation. Chromatographic conditions and system suitability are as follows: octadecylsilane chemically bonded silica is used as a filler, a flowing phase contains an organic solvent and an acidic solution, the volume percent of the organic solvent in the flowing phase is 10-30%, the volume percent of the acidic solution in the flowing phase is 70-90%, the concentration of the acidic solution is 0-0.3%, and the detection wavelength is 322-345nm. The method disclosed by the invention can be used for accurately and effectively measuring the content of verbascoside in the callicarpa nudiflora preparation, has a small error, can further improve the quality standard of the callicarpa nudiflora preparation, and can ensure the quality and clinical effect of the callicarpa nudiflora preparation.

The invention discloses a method for extraction and separation of verbascoside from rehmannia glutinosa. The method consists of: using a 75% ethanol aqueous solution to conduct heating reflux extraction on dried raw rehmannia root to obtain rehmannia root total extract, carrying out macroporous adsorption resin separation and gradient elution on the rehmannia root total extract, collecting 5-10% ethanol eluent to prepare a verbascoside crude product, and then performing separation and purification by high-speed counter-current chromatography so as to obtain a verbascoside pure product. The method provided by the invention utilizes macroporous resin to acquire a 5-10% ethanol eluent enriched material, makes full use of crude drug resources, employs high-speed counter-current chromatography for separation and purification to obtain the verbascoside standard product, realizes automation and operability of a monomer purification process, can obtain a monomer finished product with purity up to 98% or more, and has the characteristics of convenience and simplicity, high product purity, resource saving, fast speed, low toxicity, and controllable operation, etc.