66 results about "Verbascoside" patented technology

The present invention discloses a method for extracting effective active ingredients from Buddleja buddhist flower and its application. The method comprises the following steps: first subjecting the crushed Buddleja buddhist flower to ethanol reflux extraction and concentration, and then extracting the obtained Buddleja buddhist flower alcohol extract with petroleum ether, ethyl acetate, and n-butanol respectively. The remaining aqueous phase is concentrated under reduced pressure, purified by a D101 macroporous resin column, and the target product obtained after drying is structurally identified as a verbascoside monomer compound. The method of the present invention can better control the quality standards of Buddleja buddhist flower and pave the way for a more in-depth study of its pharmacological effects by effectively separating the verbascoside compounds from the Buddleja buddhist flower, thereby laying the foundation for the clinical development of new drugs with anti-tumor, antioxidant, and immunomodulatory activities.

The invention discloses a cistanche deserticola extract for improving bone mineral density, the total content of echinacoside and verbascoside in the cistanche deserticola extract is greater than 25wt%, and the cistanche deserticola extract is obtained by adding distilled water into cistanche tubulosa coarse powder for extraction, filtration, filtrate centrifugation, concentration, extraction, concentration and impurity removal, dilution, after-treatment, concentration and drying. The total content of echinacoside and verbascoside in the cistanche deserticola extract for improving the bone mineral density is 25-30 wt%, and the cistanche deserticola extract is rich in shikimic acid, phenylpropionic acid and alkaloid components, so that the cistanche deserticola extract can stably play a role in improving the bone mineral density.

The invention discloses a natural product composition and application thereof in reducing uric acid and blood sugar. The composition is prepared from luteolin, verbascoside and curcumin according to the weight ratio of 1: 3: 2. According to the invention, when luteolin, verbascoside and curcumin are combined according to a weight ratio of 1: 3: 2, the inhibitory activity on xanthine oxidase (XO) and alpha-glycosidase is the strongest, and the inhibitory activity is obviously superior to the inhibitory activity on xanthine oxidase (XO) and alpha-glycosidase when the luteolin, verbascoside and curcumin are independently used, so that the composition has a synergistic effect on reducing the level of uric acid and regulating blood sugar. The discovery provides a theoretical basis and a formula basis for developing novel natural-source functional products for reducing uric acid and reducing blood sugar, and has important application prospects and development values.

This invention discloses a method for determining the content of target components in Qingning Powder. Stir-fried Lepidium apetalum seeds, stir-fried Plantago asiatica seeds, and stir-fried Glycyrrhiza uralensis are dissolved in methanol solution, shaken well, filtered, and the filtrate is used to obtain a test solution for detection by high-performance liquid chromatography (HPLC). The three target components in the test solution are identified as: quercetin-3-O-β-D-glucose-7-O-β-D-gentiopicroside (C...). 33 H 40 O 22 ), genipinic acid (C 16 H 22 O 10 ), glycyrrhizin (C 21 H 22 The content of O9); the detection method using high performance liquid chromatography includes the following steps; Step 1: Take appropriate amounts of reference standards for quercetin-3-O-β-D-glucose-7-O-β-D-gentiopicroside, genipin glycoside, verbascoside, and glycyrrhizin, respectively, and weigh them accurately. This method for determining the content of target components in Qingning Powder, through monomer analysis combined with specific reference standards, breaks through the mixed bottleneck of the whole formula fingerprint spectrum and quickly locates the responsible medicinal materials of abnormal batches.

The invention discloses a preparation for improving male erectile function and sperm quality. The health-care food is characterized by being prepared from the following raw materials in parts by weight: 30-50 parts of herba epimedii extract, 20-40 parts of herba cistanche extract, 15-30 parts of fructus lycii extract, 18-35 parts of semen cuscutae extract, 10-25 parts of herba cynomorii extract, 8-20 parts of maca powder, 6-18 parts of raspberry extract, 0.5-2 parts of lycopene, 1-3 parts of L-carnitine and 0.3-1 part of vitamin B family (vitamin B1, vitamin B2, vitamin B6 and vitamin B12 are mixed according to the mass ratio of 1: 1: 2: 0.5). The herba epimedii extract is selected as a main component, the content of icariin is regulated to be not lower than 60%, the high-content icariin can more effectively promote sex hormone secretion, enhance sexual desire and improve erectile function, the total content of echinacoside and verbascoside in the herba cistanche extract is not lower than 45%, and the herba epimedii extract and the herba cistanche extract have the effect of promoting sex hormone secretion, enhancing sexual desire and improving erectile function. The two components have the effects of tonifying kidney yang and benefiting essence and blood, and can enhance kidney functions.

The present invention discloses a method for simultaneously detecting seven effective ingredients in Yangyin Qingfei Pills, and belongs to the technical field of chemical detection. The method for simultaneously detecting seven effective ingredients in Yangyin Qingfei Pills described in the present invention comprises the following steps: injecting the Yangyin Qingfei Pill sample solution into a liquid chromatograph, and using a segmented wavelength conversion method to perform chromatographic detection to achieve qualitative and quantitative detection of the seven effective ingredients. The present invention uses the UPLC segmented wavelength conversion method to achieve simultaneous determination of the contents of harpagine, paeoniflorin, liquiritin, verbascoside, harpagine, paeonol and glycyrrhizic acid in Yangyin Qingfei Pills, and all seven effective ingredients can be well separated without interference from other ingredients. Moreover, each component has a good linear relationship within its own linear range, and the sample recovery rate is between 95% and 105%. The detection method of the present invention is simple, accurate and effective, and can be used for quality control and overall quality evaluation of Yangyin Qingfei Pills.

The application provides a method for determining the content of sandalwood medicinal materials, decoction pieces, standard decoction and formula granules, which takes verbascoside as a control sample, and detects the test sample solution and the control sample solution through liquid chromatography, and takes acetonitrile-water with a volume ratio of (15-20):(80-85) as a mobile phase. The method can scientifically identify sandalwood medicinal materials, decoction pieces, standard decoction and formula granules, can control the internal quality of sandalwood and related preparations from the whole and macro, and can effectively guarantee the curative effect of the medicine, so that the medicinal materials and related preparations can be more regularly controlled in quality.

This invention relates to a method for preparing verbascoside and its application in promoting skin lipid production, belonging to the technical field of skin care and topical products. The method includes the following steps: Selecting dried fleshy stems of *Cistanche deserticola*, *Cistanche tubulosa*, *Cistanche salsa*, and *Cistanche salsa* (all belonging to the Orobanchaceae family), pulverizing them after pretreatment, and extracting them with 50%-80% ethanol aqueous solution under reflux for 2-4 times, 1-3 hours each time. The extracts are combined and concentrated under reduced pressure until no alcohol odor remains, obtaining a concentrated solution. The concentrated solution is passed through an AB-8 macroporous adsorption resin column, eluted sequentially with deionized water and 30%-50% ethanol aqueous solution to remove impurities, and then eluted with 60%-90% ethanol aqueous solution to remove the target component. The eluent is collected and concentrated under reduced pressure, purified by silica gel column chromatography using chloroform-methanol at a volume ratio of 5:1-2:1 as the eluent. The target fraction is collected, concentrated under reduced pressure, and freeze-dried to obtain the purified verbascoside. The beneficial effects of this invention are: verbascoside has a skin-protective effect and exhibits superior skin-care activity when applied to products.

The present invention discloses a plant whitening sunscreen composition and its preparation method and application, and relates to the field of cosmetic technology. The present invention provides a plant whitening sunscreen composition, comprising the following components in parts by weight: 0.5-5 parts of a P / O / W composite carrier, 0.1-3 parts of bakuchiol, and 0.1-3 parts of a thyme extract. The present invention stabilizes two components, pterostilbene and verbascoside, in a P / O / W pickering multiple emulsion system to form a P / O / W composite carrier, which is mixed with bakuchiol and thyme extract in specific parts by weight. On the one hand, it can achieve safe and efficient whitening by repairing, inhibiting melanin production, inhibiting melanin transport, and promoting the synergistic enhancement of multiple pathways of melanin autophagy; on the other hand, it can achieve safe and efficient whitening and sun protection, achieve the effect of "external protection and internal whitening", and can synergistically strengthen the skin's defensive barrier and improve problems such as dull skin in sensitive skin.

The invention discloses a quality control method and application of eyesight-improving and kidney-nourishing tablets, and relates to the technical field of medicines.The control method comprises the steps that firstly, a fingerprint spectrum of the eyesight-improving and kidney-nourishing tablets is established and used for quality detection of the eyesight-improving and kidney-nourishing tablets, and effective components of six medicinal materials in the eyesight-improving and kidney-nourishing tablets can be detected; meanwhile, the content of glucose, salidroside, chlorogenic acid, verbascoside, catalpol, specnuezhenide, rehmannia D, 3, 5-O-dicaffeoylquinic acid, 4, 5-O-dicaffeoylquinic acid, chrysophanol, luteolin, luteoloside, aurantio-obtusin and beta-ecdysterone in a sample can be quantitatively detected. Establishment of the fingerprint spectrum detection method of the eyesight-improving and kidney-nourishing tablets and determination of standard fingerprint spectrum information of the eyesight-improving and kidney-nourishing tablets have important significance on work in the aspects of product quality control, authenticity identification, origin traceability and the like.

The invention discloses a construction method of a finger-print spectrum of a styptic capsule and application of the styptic capsule in quality detection, and belongs to the field of Chinese patent medicine detection. The method comprises the following steps: firstly, adding 80% methanol solution into contents of different batches of Xuezhenning capsules for extraction to prepare Xuezhenning capsule test solution; the method comprises the following steps: taking ginsenoside Rg1, notoginsenoside R2, ginsenoside Rd, caffeic acid, isorhamnetin, verbascoside, aesculetin, ferulic acid, rutin and quercetin as reference substances, and preparing a reference substance solution; respectively injecting the test solution and the reference solution into a high performance liquid chromatograph to obtain corresponding chromatograms; performing similarity analysis on the chromatogram of the test solution; and finally, according to the chromatogram of the test solution and the chromatogram of the reference substance solution, combining a total ion chromatogram obtained by the high-resolution mass spectrum and a mass spectrum result map of the chemical components to construct the fingerprint spectrum of the styptic Ning capsule. The method can be used for simultaneously detecting 10 components in the haemostatic Ning capsule, and can be used for accurately, clearly and objectively evaluating the quality of the haemostatic Ning capsule.

The application discloses a capillary electrophoresis quantitative analysis method of active ingredients of Wuobi Shanyao pills. The method comprises the following steps: pretreating and purifying the powder of the test sample Wuobi Shanyao pills by MSPD-SPE, and quantitatively determining the active ingredients of the test sample, i.e., loganin, geniposidic acid, trilobatin and verbascoside by CE-UV. The MSPD with high extraction efficiency, the SPE with strong purification capacity and the CE with less consumption of organic solvents are combined for the first time, and are used for green, efficient and accurate content determination of the four components (loganin, geniposidic acid, trilobatin and verbascoside) in Wuobi Shanyao pills. In the sample preparation process and in the CE separation and determination, the consumption of organic solvents is far less than the amount consumed in the prior art.

The invention provides an effective part of callicarpa nudiflora. Specifically, the invention provides an effective part of callicarpa nudiflora and a preparation method and application thereof. The effective part of callicarpa nudiflora and verbascoside are found to have excellent biological activity for the first time, the effective part of callicarpa nudiflora and verbascoside can be used as an external preparation to effectively treat diabetic wound ulcer, the preparation method is simple and low in cost, the whole process is carried out at low temperature, active ingredients are not damaged, and the extraction efficiency of the active ingredients is high.

The invention belongs to the technical field of functional membrane material preparation, and particularly relates to a preparation method of a molecularly imprinted composite membrane and application of the molecularly imprinted composite membrane in natural product separation. According to the method, firstly, dendritic hollow carbon (DHCs) and a polyvinylidene fluoride (PVDF) matrix are compounded to prepare a membrane through a physical blending method, then a specific molecularly imprinted layer is constructed on the surface of the membrane, and finally the carbon-doped molecularly imprinted composite membrane (CMICMs) with the recognition function is obtained. According to the design, the separation performance is synergistically enhanced; specific recognition of verbascoside is realized by the surface imprinting layer through hydrogen-bond interaction; and the DHCs uniformly dispersed in the membrane remarkably improve the binding capacity of target molecules by virtue of unique mesoporous channels and hollow cavity structures of the DHCs. And a new effective strategy is provided for efficient separation of verbascoside.

The invention discloses a fingerprint spectrum detection method of a consistent decoction reference sample and application, and relates to the technical field of traditional Chinese medicine detection. According to the fingerprint detection method, a test solution is determined and analyzed by adopting a high performance liquid chromatography, and the conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 chromatographic column; the column temperature is 28-32 DEG C; the detection wavelength is 0 to 25 minutes, and the wavelength is 278 to 282nm; in 25-66 minutes, the wavelength is 318-322 nm; the time is 66 to 80 minutes, and the wavelength is 278 to 282nm; the time is 80 to 90 minutes, and the wavelength is 318 to 322 nm; the mobile phase A is acetonitrile, and the mobile phase B is a 0.09-0.11 wt% phosphoric acid solution; and eluting by adopting a gradient elution procedure. According to the method, digitonin C, 4-cumaric acid, ferulic acid, rutin, verbascoside, senkyunolide I and ligustilide in the consistent decoction can be detected at a time, and the method is high in accuracy, good in repeatability, high in durability and good in specificity.

The invention relates to the technical field of component detection of clerodendrum japonicum, in particular to a method for determining the content of active substances in clerodendrum japonicum based on a high performance liquid chromatography method. The invention provides a method for determining the content of active substances in clerodendrum japonicum based on high performance liquid chromatography, which comprises the following steps: (1) mixing the clerodendrum japonicum medicinal material with ethanol, and carrying out ultrasonic treatment to obtain a test solution; the method comprises the following steps: respectively taking a chlorogenic acid solution, an apigenin-7-O-diglucuronic acid solution, a verbascoside solution and an acacetin-7-O-[beta-D-glucopyranuronyl (1-> 2)-O-D-glucopyranuronide] solution as reference substance solutions; and (2) performing high performance liquid chromatography detection on the test solution and the reference solution obtained in the step (1). The method can be used for simultaneously detecting the contents of four active components in clerodendrum japonicum, is low in detection limit and wide in linear range, and ensures the accuracy and reliability of a detection result.

The invention discloses whitening and brightening tone-up cream and a preparation method thereof, and belongs to the technical field of tone-up cream. Comprising a phase A, a phase B, a phase C and a phase D, the phase C comprises whitening and brightening factors, and the whitening and brightening factors comprise a radix paeoniae alba extract, schizophyllan, a saxifraga stolonifera extract and verbascoside; the four active ingredients of the radix paeoniae alba extract, the schizophyllan, the saxifraga stolonifera extract and the verbascoside form a synergistic whitening system, melanin generation is inhibited, free radicals are removed, melanin deposition is reduced, the overall whitening and skin brightening effect is enhanced, and the multiple effects of whitening, skin brightening, health, nourishing and repairing of an existing tone-up cream product are improved.

The invention provides a method for analyzing an anti-eczema effect target spot of traumatology based on network pharmacology. The method comprises the following steps: firstly, screening out seven representative active components through a SwisSADME platform, predicting by integrating SwisTarget Prediction, SEA and ChEMBL databases to obtain 367 drug targets, and analyzing to obtain 82 intersection targets; a PPI network is constructed based on an STRING database, and 10 core targets such as IL6, TNF and EGFR are screened out through topology analysis. GO / KEGG enrichment analysis reveals that the GO / KEGG is significantly enriched in the processes of IL-17 signal pathway, epidermal barrier repair and the like. Molecular docking verification proves that isofraxidin, scopoletin and verbenin are strongly combined with EGFR (epidermal growth factor receptor) targets. According to the method, a multi-component-multi-target-multi-channel synergistic eczema treatment mechanism of traumatology is systematically analyzed, and a theoretical basis is provided for preparation optimization.

The invention discloses an osmanthus fragrans extract for preventing premature ovarian failure and a preparation method and application thereof.The extract is prepared from osmanthus fragrans through microwave fixation, gradient temperature weak acid extraction, membrane separation and concentration, low-temperature drying and other pure physical processes, effective components can be prevented from being damaged, the process is stable, green and efficient, the active components are definite, and the preparation method is simple. Wherein the sum of the contents of the salidroside, the verbascoside and the ligustroside is not less than 20%. Animal experiments show that AMH, FSH and LH hormone levels in mice suffering from premature ovarian failure induced by cyclophosphamide can be remarkably improved, the ovarian function can be effectively improved, all index components including salidroside, verbascoside and ligustrum glycoside have a synergistic interaction effect, a safe and effective solution scheme derived from natural plants is provided for preventing premature ovarian failure, and the application prospect is wide. The method can be widely applied to preparation of various products.

The present application relates to the technical field of fermentation, and especially relates to a preparation method for extracting whitening and anti-aging active ingredient verbascoside from lemon verbena fermentation substrate, the separation method of the present application is three times of separation and purification, first, macroporous resin D101 is used, then, silica gel column chromatography resin is used, then, dextran gel resin is used, and finally, monomeric compound is obtained through recrystallization; the content of verbascoside in the initial raw material is increased from 1.57-4.11% in the initial lemon verbena stem and leaf to 2.83-7.75%, so that the content of lemon verbena in the starting raw material is increased by 2-5 times; the present application uses three-step separation and purification, so that monomeric compound verbascoside with a monomer purity of 90.42-99.17% can be obtained; the present application increases the final yield by 2-3 times, from the existing 3-5% to 7.28-11.33%, so that the technical problems of the preparation method in the prior art, such as high price, low content, complex separation and purification process, low recovery rate and low monomer purity, are solved.

The application provides a method for rapidly screening anti-tumor metastasis active compounds, relates to the technical field of tumor treatment, and uses a statistical data analysis method to rapidly screen active monomer compounds for resisting tumor metastasis from traditional Chinese medicines containing several hundred compounds, and based on the method, nine components are extracted from traditional Chinese medicine forsythia suspensa; the scratch activity group of the nine components is combined with compound information obtained through mass spectrum analysis; and through statistical data analysis, the active monomer compound verbascoside VB contained in forsythia suspensa for inhibiting tumor metastasis is rapidly screened.

The invention discloses application of verbascoside in preparation of a product for preventing and treating influenza virus infection, and relates to the technical field of biological medicines. Through molecular docking screening, cytotoxicity experiments and antiviral activity verification, the application value of verbascoside in prevention and treatment of influenza virus infection is defined, and the technical effect is remarkable. In the aspect of safety, the verbascoside has no obvious influence on the survival rate of MDCK cells at the concentration of 200 mu M, the concentration of the verbascoside is far higher than the clinical potential medication concentration, and the verbascoside has no obvious cytotoxicity and good biocompatibility. In the aspect of antiviral activity, verbascoside shows an efficient inhibition effect on influenza viruses (WSN strains and FM1 strains), the IC50 value is as low as 28.3 mu M, and the inhibition effect is superior to that of part of natural components. In an application scene, verbascoside can be prepared into various drug dosage forms and can also be used as an active ingredient of a disinfectant, and a novel safe, efficient and multifunctional flu prevention and control scheme is provided for the field of biological medicine.

This invention discloses the application of verbascoside in the preparation of drugs for treating obesity and related metabolic disorders, relating to the field of biopharmaceuticals. The drug upregulates the expression of phosphorylated AMP-activated protein kinase α and carnitine palmitoyltransferase-1α, while downregulating the expression of sterol regulatory element-binding protein-1c, fatty acid synthase, and acetyl-CoA carboxylase. This application is the first systematic study of the comprehensive intervention effect of verbascoside on diet-induced obesity and related metabolic disorders. In an in vivo obese mouse model, it was demonstrated that verbascoside can significantly inhibit excessive weight gain induced by a high-fat diet, reduce fasting blood glucose and fasting insulin levels, improve dyslipidemia indicators including triglycerides, total cholesterol, non-esterified fatty acids, and low-density lipoprotein cholesterol, enhance insulin sensitivity, and alleviate macrovesicular steatosis and hepatocellular damage.

The invention discloses an application of a verbascoside compound in preparation of a medicine for improving a cardiac function, and belongs to the technical field of biological medicines. The verbascoside disclosed by the invention has certain effects of regulating the cardiac diastolic and systolic functions of model mice, improving cardiac hemodynamics and protecting cardiac functions, has relatively good activity of reducing myocardial fibrosis, and can prevent cardiovascular complications induced by diabetes mellitus.

The application discloses a kind of Cistanche multi-field coupling combined drying method and preparation process, it is related to the technical field of traditional Chinese medicinal materials processing, including the way of temperature field, humidity field, microwave field, vacuum field and airflow field five field synergic coupling, design raw material pretreatment, low-temperature air field pre-drying, multi-field coupling main drying, low-temperature vacuum final drying and post-processing ladder type continuous process, realize the rapid, uniform, low-temperature dehydration of Cistanche.The process greatly shortens drying period, reduces unit product energy consumption, can maximize the core active ingredients such as echinacoside, verbascoside in desert Cistanche, the obtained finished product color is uniform, no burnt paste no hard core, rehydration is excellent.The drying efficiency and energy consumption of the application are considered, the ladder type drying design of the process through five field synergic coupling, greatly shortens the drying period of Cistanche, and realizes the efficient use of energy, compared with traditional drying process unit product energy consumption reduces more than 30%, adapts to the cost control demand of industrialized large-scale production.

The invention provides a traditional Chinese medicine composition, a preparation method thereof and application of the traditional Chinese medicine composition in preparation of hypoglycemic products, and relates to the technical field of traditional Chinese medicines. The invention discloses a traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw materials: 4-8 parts of ginseng stem and leaf saponin, 8-16 parts of astragalus polysaccharide, 6-12 parts of astragaloside, 8-16 parts of catalpol, 6-12 parts of rehmannia D, 8-16 parts of verbascoside, 3-5 parts of raspberry polysaccharide, 4-8 parts of ellagic acid, 4-8 parts of pachymaran, 5-10 parts of pachymic acid, 8-16 parts of lycium barbarum polysaccharide, 40-62 parts of schisandra chinensis, 20-40 parts of Chinese yam, 40-62 parts of radix ophiopogonis, 40-62 parts of radix trichosanthis and 40-62 parts of rhizoma alismatis. The traditional Chinese medicine raw materials are pretreated by using the high-voltage pulsed electric field, and then ethanol extraction treatment is performed twice, so that the yield of the traditional Chinese medicine extract is high, and the traditional Chinese medicine composition obtained by mixing the traditional Chinese medicine extract with other active ingredients has an excellent effect of treating type II diabetes mellitus.

The present invention belongs to the technical field of traditional Chinese medicine analysis, and in particular relates to a method for constructing a characteristic spectrum of Tongfengding Capsules. The present invention establishes HPLC characteristic spectrums for Tongfengding Capsules at wavelengths of 270nm and 320nm, respectively. At a wavelength of 270nm, 8 characteristic peaks are identified, and gallic acid, maleinic acid, and gentiopicroside are controlled; at a wavelength of 320nm, 11 characteristic peaks are identified, and gentiopicroside, paeoniflorin, verbascoside, and tetrahydropalmatine are controlled, thereby fully and comprehensively displaying the chemical composition characteristics of Tongfengding Capsules. In addition, the present invention investigates the extraction solvent and extraction method of the sample, and the type and concentration of the acid added in the mobile phase. At wavelengths of 270nm and 320nm, the relative retention time changes of each peak are small, and the RDS is less than 2.0%.

The application provides a molecular imprinting composite film for selectively separating phenylethanoid glycosides, wherein the matrix of the molecular imprinting composite film is polypropylene, polyethersulfone, polycarbonate, cellulose acetate or polyvinylidene fluoride; and a verbascoside imprinting layer or a trilobatin imprinting layer is synthesized on the surface of the composite film. Compared with the prior art, the application has the following advantages: the composite film provided by the application has a coral-like structure, and has the advantages of easy recycling, no pollution, low energy consumption, excellent separation performance, application in continuous separation process and the like, and effectively solves the problems of few types, low purity, difficult recycling, high energy consumption and secondary pollution; the composite film is a molecular imprinting composite film for efficiently separating phenylethanoid glycosides, which is designed by combining the molecular imprinting technology and the membrane separation method in the technical field of separation of effective components of natural products, and is expected to provide a new application idea and method for high-efficiency separation of phenylethanoid glycosides in effective components of natural products.

The invention relates to the technical field of co-crystals, in particular to a co-crystal of verbascoside and mannitol and a preparation method and application of the co-crystal, an XRD (X-Ray Diffraction) pattern of the co-crystal has characteristic diffraction peaks at least at positions where 2 theta angles are 13.708 degrees, 17.329 degrees, 18.810 degrees, 19.929 degrees, 20.488 degrees, 21.404 degrees, 27.066 degrees, 28.218 degrees, 33.201 degrees and 43.963 degrees, and error tolerance of + / -0.2 degrees exists; a characteristic endothermic peak exists at least at 166 DEG C in a DSC spectrum, and an error tolerance of + / -0.2 DEG C exists. The preparation method comprises the following steps: dissolving verbascoside and mannitol in water, standing, crystallizing, filtering and drying to obtain the eutectic crystal which can be applied to cosmetics, health care products, foods and medicines. The eutectic crystal provided by the invention has the advantages of no irritation, easy absorption and good stability.

The application describes a large scale process for the in vitro production of an olive cell culture. The application further describes a composition in a form of a powder comprising olive fruit / leaf cells grown in vitro. The cell culture of olive cells manufactured in vitro in a large scale according to the process of the invention comprises high level of verbascoside with undetectable levels of tyrosol, hydroxytyrosol, and oleuropein.