41 results about "Verbascoside" patented technology

The invention discloses a cistanche deserticola extract for improving bone mineral density, the total content of echinacoside and verbascoside in the cistanche deserticola extract is greater than 25wt%, and the cistanche deserticola extract is obtained by adding distilled water into cistanche tubulosa coarse powder for extraction, filtration, filtrate centrifugation, concentration, extraction, concentration and impurity removal, dilution, after-treatment, concentration and drying. The total content of echinacoside and verbascoside in the cistanche deserticola extract for improving the bone mineral density is 25-30 wt%, and the cistanche deserticola extract is rich in shikimic acid, phenylpropionic acid and alkaloid components, so that the cistanche deserticola extract can stably play a role in improving the bone mineral density.

The invention discloses a natural product composition and application thereof in reducing uric acid and blood sugar. The composition is prepared from luteolin, verbascoside and curcumin according to the weight ratio of 1: 3: 2. According to the invention, when luteolin, verbascoside and curcumin are combined according to a weight ratio of 1: 3: 2, the inhibitory activity on xanthine oxidase (XO) and alpha-glycosidase is the strongest, and the inhibitory activity is obviously superior to the inhibitory activity on xanthine oxidase (XO) and alpha-glycosidase when the luteolin, verbascoside and curcumin are independently used, so that the composition has a synergistic effect on reducing the level of uric acid and regulating blood sugar. The discovery provides a theoretical basis and a formula basis for developing novel natural-source functional products for reducing uric acid and reducing blood sugar, and has important application prospects and development values.

This invention discloses a method for determining the content of target components in Qingning Powder. Stir-fried Lepidium apetalum seeds, stir-fried Plantago asiatica seeds, and stir-fried Glycyrrhiza uralensis are dissolved in methanol solution, shaken well, filtered, and the filtrate is used to obtain a test solution for detection by high-performance liquid chromatography (HPLC). The three target components in the test solution are identified as: quercetin-3-O-β-D-glucose-7-O-β-D-gentiopicroside (C...). 33 H 40 O 22 ), genipinic acid (C 16 H 22 O 10 ), glycyrrhizin (C 21 H 22 The content of O9); the detection method using high performance liquid chromatography includes the following steps; Step 1: Take appropriate amounts of reference standards for quercetin-3-O-β-D-glucose-7-O-β-D-gentiopicroside, genipin glycoside, verbascoside, and glycyrrhizin, respectively, and weigh them accurately. This method for determining the content of target components in Qingning Powder, through monomer analysis combined with specific reference standards, breaks through the mixed bottleneck of the whole formula fingerprint spectrum and quickly locates the responsible medicinal materials of abnormal batches.

This invention relates to a method for preparing verbascoside and its application in promoting skin lipid production, belonging to the technical field of skin care and topical products. The method includes the following steps: Selecting dried fleshy stems of *Cistanche deserticola*, *Cistanche tubulosa*, *Cistanche salsa*, and *Cistanche salsa* (all belonging to the Orobanchaceae family), pulverizing them after pretreatment, and extracting them with 50%-80% ethanol aqueous solution under reflux for 2-4 times, 1-3 hours each time. The extracts are combined and concentrated under reduced pressure until no alcohol odor remains, obtaining a concentrated solution. The concentrated solution is passed through an AB-8 macroporous adsorption resin column, eluted sequentially with deionized water and 30%-50% ethanol aqueous solution to remove impurities, and then eluted with 60%-90% ethanol aqueous solution to remove the target component. The eluent is collected and concentrated under reduced pressure, purified by silica gel column chromatography using chloroform-methanol at a volume ratio of 5:1-2:1 as the eluent. The target fraction is collected, concentrated under reduced pressure, and freeze-dried to obtain the purified verbascoside. The beneficial effects of this invention are: verbascoside has a skin-protective effect and exhibits superior skin-care activity when applied to products.

The invention discloses a construction method of a finger-print spectrum of a styptic capsule and application of the styptic capsule in quality detection, and belongs to the field of Chinese patent medicine detection. The method comprises the following steps: firstly, adding 80% methanol solution into contents of different batches of Xuezhenning capsules for extraction to prepare Xuezhenning capsule test solution; the method comprises the following steps: taking ginsenoside Rg1, notoginsenoside R2, ginsenoside Rd, caffeic acid, isorhamnetin, verbascoside, aesculetin, ferulic acid, rutin and quercetin as reference substances, and preparing a reference substance solution; respectively injecting the test solution and the reference solution into a high performance liquid chromatograph to obtain corresponding chromatograms; performing similarity analysis on the chromatogram of the test solution; and finally, according to the chromatogram of the test solution and the chromatogram of the reference substance solution, combining a total ion chromatogram obtained by the high-resolution mass spectrum and a mass spectrum result map of the chemical components to construct the fingerprint spectrum of the styptic Ning capsule. The method can be used for simultaneously detecting 10 components in the haemostatic Ning capsule, and can be used for accurately, clearly and objectively evaluating the quality of the haemostatic Ning capsule.

The application discloses a capillary electrophoresis quantitative analysis method of active ingredients of Wuobi Shanyao pills. The method comprises the following steps: pretreating and purifying the powder of the test sample Wuobi Shanyao pills by MSPD-SPE, and quantitatively determining the active ingredients of the test sample, i.e., loganin, geniposidic acid, trilobatin and verbascoside by CE-UV. The MSPD with high extraction efficiency, the SPE with strong purification capacity and the CE with less consumption of organic solvents are combined for the first time, and are used for green, efficient and accurate content determination of the four components (loganin, geniposidic acid, trilobatin and verbascoside) in Wuobi Shanyao pills. In the sample preparation process and in the CE separation and determination, the consumption of organic solvents is far less than the amount consumed in the prior art.

The invention provides an effective part of callicarpa nudiflora. Specifically, the invention provides an effective part of callicarpa nudiflora and a preparation method and application thereof. The effective part of callicarpa nudiflora and verbascoside are found to have excellent biological activity for the first time, the effective part of callicarpa nudiflora and verbascoside can be used as an external preparation to effectively treat diabetic wound ulcer, the preparation method is simple and low in cost, the whole process is carried out at low temperature, active ingredients are not damaged, and the extraction efficiency of the active ingredients is high.

The invention discloses a fingerprint spectrum detection method of a consistent decoction reference sample and application, and relates to the technical field of traditional Chinese medicine detection. According to the fingerprint detection method, a test solution is determined and analyzed by adopting a high performance liquid chromatography, and the conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 chromatographic column; the column temperature is 28-32 DEG C; the detection wavelength is 0 to 25 minutes, and the wavelength is 278 to 282nm; in 25-66 minutes, the wavelength is 318-322 nm; the time is 66 to 80 minutes, and the wavelength is 278 to 282nm; the time is 80 to 90 minutes, and the wavelength is 318 to 322 nm; the mobile phase A is acetonitrile, and the mobile phase B is a 0.09-0.11 wt% phosphoric acid solution; and eluting by adopting a gradient elution procedure. According to the method, digitonin C, 4-cumaric acid, ferulic acid, rutin, verbascoside, senkyunolide I and ligustilide in the consistent decoction can be detected at a time, and the method is high in accuracy, good in repeatability, high in durability and good in specificity.

The invention discloses whitening and brightening tone-up cream and a preparation method thereof, and belongs to the technical field of tone-up cream. Comprising a phase A, a phase B, a phase C and a phase D, the phase C comprises whitening and brightening factors, and the whitening and brightening factors comprise a radix paeoniae alba extract, schizophyllan, a saxifraga stolonifera extract and verbascoside; the four active ingredients of the radix paeoniae alba extract, the schizophyllan, the saxifraga stolonifera extract and the verbascoside form a synergistic whitening system, melanin generation is inhibited, free radicals are removed, melanin deposition is reduced, the overall whitening and skin brightening effect is enhanced, and the multiple effects of whitening, skin brightening, health, nourishing and repairing of an existing tone-up cream product are improved.

The invention provides a method for analyzing an anti-eczema effect target spot of traumatology based on network pharmacology. The method comprises the following steps: firstly, screening out seven representative active components through a SwisSADME platform, predicting by integrating SwisTarget Prediction, SEA and ChEMBL databases to obtain 367 drug targets, and analyzing to obtain 82 intersection targets; a PPI network is constructed based on an STRING database, and 10 core targets such as IL6, TNF and EGFR are screened out through topology analysis. GO / KEGG enrichment analysis reveals that the GO / KEGG is significantly enriched in the processes of IL-17 signal pathway, epidermal barrier repair and the like. Molecular docking verification proves that isofraxidin, scopoletin and verbenin are strongly combined with EGFR (epidermal growth factor receptor) targets. According to the method, a multi-component-multi-target-multi-channel synergistic eczema treatment mechanism of traumatology is systematically analyzed, and a theoretical basis is provided for preparation optimization.

The invention discloses an osmanthus fragrans extract for preventing premature ovarian failure and a preparation method and application thereof.The extract is prepared from osmanthus fragrans through microwave fixation, gradient temperature weak acid extraction, membrane separation and concentration, low-temperature drying and other pure physical processes, effective components can be prevented from being damaged, the process is stable, green and efficient, the active components are definite, and the preparation method is simple. Wherein the sum of the contents of the salidroside, the verbascoside and the ligustroside is not less than 20%. Animal experiments show that AMH, FSH and LH hormone levels in mice suffering from premature ovarian failure induced by cyclophosphamide can be remarkably improved, the ovarian function can be effectively improved, all index components including salidroside, verbascoside and ligustrum glycoside have a synergistic interaction effect, a safe and effective solution scheme derived from natural plants is provided for preventing premature ovarian failure, and the application prospect is wide. The method can be widely applied to preparation of various products.

The application provides a method for rapidly screening anti-tumor metastasis active compounds, relates to the technical field of tumor treatment, and uses a statistical data analysis method to rapidly screen active monomer compounds for resisting tumor metastasis from traditional Chinese medicines containing several hundred compounds, and based on the method, nine components are extracted from traditional Chinese medicine forsythia suspensa; the scratch activity group of the nine components is combined with compound information obtained through mass spectrum analysis; and through statistical data analysis, the active monomer compound verbascoside VB contained in forsythia suspensa for inhibiting tumor metastasis is rapidly screened.

This invention discloses the application of verbascoside in the preparation of drugs for treating obesity and related metabolic disorders, relating to the field of biopharmaceuticals. The drug upregulates the expression of phosphorylated AMP-activated protein kinase α and carnitine palmitoyltransferase-1α, while downregulating the expression of sterol regulatory element-binding protein-1c, fatty acid synthase, and acetyl-CoA carboxylase. This application is the first systematic study of the comprehensive intervention effect of verbascoside on diet-induced obesity and related metabolic disorders. In an in vivo obese mouse model, it was demonstrated that verbascoside can significantly inhibit excessive weight gain induced by a high-fat diet, reduce fasting blood glucose and fasting insulin levels, improve dyslipidemia indicators including triglycerides, total cholesterol, non-esterified fatty acids, and low-density lipoprotein cholesterol, enhance insulin sensitivity, and alleviate macrovesicular steatosis and hepatocellular damage.

The application discloses a kind of Cistanche multi-field coupling combined drying method and preparation process, it is related to the technical field of traditional Chinese medicinal materials processing, including the way of temperature field, humidity field, microwave field, vacuum field and airflow field five field synergic coupling, design raw material pretreatment, low-temperature air field pre-drying, multi-field coupling main drying, low-temperature vacuum final drying and post-processing ladder type continuous process, realize the rapid, uniform, low-temperature dehydration of Cistanche.The process greatly shortens drying period, reduces unit product energy consumption, can maximize the core active ingredients such as echinacoside, verbascoside in desert Cistanche, the obtained finished product color is uniform, no burnt paste no hard core, rehydration is excellent.The drying efficiency and energy consumption of the application are considered, the ladder type drying design of the process through five field synergic coupling, greatly shortens the drying period of Cistanche, and realizes the efficient use of energy, compared with traditional drying process unit product energy consumption reduces more than 30%, adapts to the cost control demand of industrialized large-scale production.

The invention provides a traditional Chinese medicine composition, a preparation method thereof and application of the traditional Chinese medicine composition in preparation of hypoglycemic products, and relates to the technical field of traditional Chinese medicines. The invention discloses a traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw materials: 4-8 parts of ginseng stem and leaf saponin, 8-16 parts of astragalus polysaccharide, 6-12 parts of astragaloside, 8-16 parts of catalpol, 6-12 parts of rehmannia D, 8-16 parts of verbascoside, 3-5 parts of raspberry polysaccharide, 4-8 parts of ellagic acid, 4-8 parts of pachymaran, 5-10 parts of pachymic acid, 8-16 parts of lycium barbarum polysaccharide, 40-62 parts of schisandra chinensis, 20-40 parts of Chinese yam, 40-62 parts of radix ophiopogonis, 40-62 parts of radix trichosanthis and 40-62 parts of rhizoma alismatis. The traditional Chinese medicine raw materials are pretreated by using the high-voltage pulsed electric field, and then ethanol extraction treatment is performed twice, so that the yield of the traditional Chinese medicine extract is high, and the traditional Chinese medicine composition obtained by mixing the traditional Chinese medicine extract with other active ingredients has an excellent effect of treating type II diabetes mellitus.

The application provides a molecular imprinting composite film for selectively separating phenylethanoid glycosides, wherein the matrix of the molecular imprinting composite film is polypropylene, polyethersulfone, polycarbonate, cellulose acetate or polyvinylidene fluoride; and a verbascoside imprinting layer or a trilobatin imprinting layer is synthesized on the surface of the composite film. Compared with the prior art, the application has the following advantages: the composite film provided by the application has a coral-like structure, and has the advantages of easy recycling, no pollution, low energy consumption, excellent separation performance, application in continuous separation process and the like, and effectively solves the problems of few types, low purity, difficult recycling, high energy consumption and secondary pollution; the composite film is a molecular imprinting composite film for efficiently separating phenylethanoid glycosides, which is designed by combining the molecular imprinting technology and the membrane separation method in the technical field of separation of effective components of natural products, and is expected to provide a new application idea and method for high-efficiency separation of phenylethanoid glycosides in effective components of natural products.

The application describes a large scale process for the in vitro production of an olive cell culture. The application further describes a composition in a form of a powder comprising olive fruit / leaf cells grown in vitro. The cell culture of olive cells manufactured in vitro in a large scale according to the process of the invention comprises high level of verbascoside with undetectable levels of tyrosol, hydroxytyrosol, and oleuropein.

The invention relates to a method for constructing HPLC characteristic chromatograms of a Yumai decoction reference sample, two sets of HPLC methods are adopted to comprehensively characterize component characteristics of four medicines in a Yumai decoction prescription, the first set of HPLC characteristic chromatogram determines 10 characteristic peaks, and the first set of HPLC characteristic chromatogram belongs to six characteristic peaks of the medicine flavor of prepared rehmannia root, and the second set of HPLC characteristic chromatogram belongs to six characteristic peaks of the medicine flavor of Yumai decoction. The components are respectively catalpol, adenosine, 5-hydroxymethylfurfural, rehmannia D, verbascoside and isoacteoside; the rhizoma anemarrhenae has three characteristic peaks, namely neomangiferin, mangiferin and isomangiferin; the first set of HPLC characteristic chromatogram determines five characteristic peaks which belong to beta-ecdysterone, the second set of HPLC characteristic chromatogram determines five characteristic peaks which belong to five characteristic peaks of radix ophiopogonis, and two peaks of the characteristic peaks are identified to be methylophiopogonanone A and methylophiopogonanone B respectively; the two sets of specific chromatogram methods established by the invention have good performance and better pertinence, can comprehensively characterize the component characteristics of the four medicines in the Yumai decoction prescription, and can effectively monitor the quality of the Yumai decoction preparation.

The invention discloses a medicine detection method, which can simultaneously determine the contents of beta-asarone, schizandrin, hyperoside, verbenin, morinda officinalis verbenin, glycyrrhizic acid and other components in a medicine, and can be used for quality control of the medicine.

The present application belongs to the technical field of detection, and relates to a detection method for multiple active ingredients of Shishi Tonglin capsules, comprising simultaneously determining the contents of salvianolic acid B, protodioscin, dioscin, hederagenin and tanshinone II A in Shishi Tonglin capsules based on UPLC-QAMS method, or / and simultaneously determining the contents of salvianolic acid B, verbascoside, berberine hydrochloride, pachymic acid A and tanshinone II A in Shishi Tonglin capsules based on HPLC-QAMS method. The present application utilizes the method of combining liquid chromatography with one measurement multiple evaluation, determines one stable, easily obtained and low-priced active ingredient, calculates the contents of other ingredients by using the relative correction factor method, realizes the simultaneous determination of multiple ingredients in Shishi Tonglin capsules, and has the advantages of simple determination method, good accuracy of detection results and reduced detection cost.

This invention discloses a method for detecting a Shenqi Ma Rong Tang composition. The method employs high-performance liquid chromatography (HPLC), including the preparation of a reference solution, a test solution, and a mixed reference solution, as well as HPLC detection steps. By detecting the test solution and the mixed reference solution, a characteristic chromatogram of the Shenqi Ma Rong Tang composition is established. Nine common chromatographic peaks are selected as characteristic peaks, with echinacoside as the reference peak. The relative retention time and relative peak area of ​​each characteristic peak are calculated, and the corresponding components are identified. The components corresponding to the nine characteristic peaks are adenosine, amygdalin, echinacoside, erythritol, verbascoside, gentianin, gentianin, and cassia seed extract. This method can comprehensively reflect the overall chemical characteristics of the Shenqi Ma Rong Tang composition and can be used for its quality control.

The invention discloses a screening method of a traditional Chinese medicine monomer composition for resisting heart failure. The method comprises the following steps: 1, carrying out water extraction and enrichment on astragalus membranaceus and monkshood to obtain astragalus membranaceus and monkshood soup enriched components; 2, synthesizing myocardial mitochondrial membrane coated magnetic beads and myocardial lysosome membrane coated magnetic beads; 3, gathering enriched components of the astragalus mongholicus-monkshood decoction, and carrying out ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry to obtain components of songorine, neoline, calycosin and formononetin as traditional Chinese medicine monomer composition; the traditional Chinese medicine monomer composition is prepared by dissolving songlin, neoline, calycosin and formononetin in a sodium carboxymethyl cellulose solution. The synthesized myocardial mitochondrial membrane coated magnetic beads and the myocardial lysosome membrane coated magnetic beads are used for adsorbing and identifying active ingredients in the astragalus mongholicus-monkshood decoction, the effective ingredient composition for resisting heart failure is successfully screened, and the preparation method is simple and easy to implement; the traditional Chinese medicine monomer composition plays a role in preparing anti-heart-failure medicines by improving heart functions and the like.

The application discloses a kind of HPLC fingerprint detection methods of traditional Chinese medicine, the traditional Chinese medicine is made of ten traditional Chinese medicinal materials such as radix codonopsis, atractylodes, poria, licorice, angelica, chuanxiong, white peony root, prepared rehmannia, astragalus, cassia, with the effect of tonifying qi and blood, the fingerprint established in the application includes 11 common characteristic peaks, respectively belong to ten medicinal materials, so it can identify all ten medicinal materials in traditional Chinese medicine, and also identifies 5 components therein, which are glycyrrhizin, ammonium glycyrrhizinate, verbascoside, calycosin-7-glucoside and astragaloside A respectively, so the application can more comprehensively control drug quality, solve the phenomenon of fake and inferior drugs, and ensure the safety and effectiveness of clinical use.

The application belongs to the technical field of detection, and relates to a quality detection method of Shuishitonglin capsules, which comprises identifying Poria cocos, Acorus gramineus Soland, Qingdai, Phellodendri Amurensis Cortex, Salvia miltiorrhiza and Atractylodes lancea in the Shuishitonglin capsules by adopting thin layer chromatography; and further comprises detecting the content of berberine hydrochloride, tanshinone II A, salvianolic acid B, geniposidic acid and / or verbascoside in the Shuishitonglin capsules by adopting high performance liquid chromatography. The quality detection method of the Shuishitonglin capsules can accurately identify Poria cocos, Acorus gramineus Soland, Qingdai, Phellodendri Amurensis Cortex, Salvia miltiorrhiza and Atractylodes lancea in the Shuishitonglin capsules, and detect the content of berberine hydrochloride, tanshinone II A, salvianolic acid B, geniposidic acid and / or verbascoside contained in the Shuishitonglin capsules, and has high accuracy, good precision, high sensitivity, good reproducibility, simple operation, short time and the like.

The invention provides a composition for improving inflammatory aging and application thereof. The composition is formed by compounding ergothioneine and verbascoside according to the mass ratio of 1: 10-10: 1, the interaction of a binary mixture is calculated by adopting a joint index (CI) method, and the synergistic anti-inflammatory effect of ergothioneine and verbascoside is determined to relieve inflammatory aging. The composition can improve inflammation by inhibiting the levels of cell inflammatory factors IL-1beta, IL-6 and TNF-alpha; the influence on inflammation is weakened by inhibiting the antioxidant activity (ROS, MDA and GSSG) of cells. Furthermore, the composition can inhibit cell ferroptosis in a targeted manner, reduce the lipid peroxidation level and the cell ferrous iron content, reduce cell aging and maintain the redox state of cells. The composition provided by the invention has a remarkable targeted inhibition effect on cell ferroptosis, and has important significance on inhibition of senescence related to ferroptosis and product development.

The invention provides an application of calycosin-7-glucoside in preparation of a medicine for relieving ulcerative colitis. The invention finds that the calycosin-7-glucoside can relieve ulcerative colitis by adjusting the activity of COX-II enzyme. On one hand, the calycosin glucoside promotes the homeostasis of intestinal epithelial cells by increasing the expression quantity of a downstream product, namely 12 (S)-HHT, of COX-II enzyme in vivo; on the other hand, generation of TxA2 can be promoted, the blood coagulation process is accelerated, and then the pathological state of ulcerative colitis is improved. Animal experiments further find that the calycosin-7-glucoside can significantly inhibit the UC inflammatory reaction and improve related disease symptoms at a low oral dosage. The calycosin-7-glucoside has the advantages of small toxic and side effects, low dosage and the like, and a new treatment scheme is provided for treatment of ulcerative colitis.

The invention discloses an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of a peach-astragalus hematopoiesis capsule as well as construction and application thereof, and belongs to the technical field of quality control of traditional Chinese medicine preparations. According to the method, high performance liquid chromatography and mass spectrometry are combined, and formononin, formononetin, calycosin 7-O-beta-D-glucoside, calycosin, ferulic acid, rutin and 5-hydroxymethylfurfural are used as reference substances; and the fingerprint spectrum is successfully constructed by detecting different batches of the peach-astragalus hematopoiesis capsules. The method is simple and easy to implement, high in precision, good in reproducibility, accurate and reliable, at least seven components can be confirmed at a time under the same chromatographic condition, the detection efficiency is greatly improved, and time and cost are saved; quality monitoring can be accurately, rapidly and simultaneously carried out on a plurality of characteristic components of the peach-stilbene hematogenic capsule, and a powerful guarantee is provided for controlling the quality and ensuring the clinical curative effect of the peach-stilbene hematogenic capsule.

The application belongs to the technical field of cell culture, and particularly relates to a kind of extracellular vesicles containing active mitochondria and a preparation method thereof.The preparation method specifically comprises the following steps: culturing mitochondrial donor cells until the fusion degree reaches 70-80%, discarding the original culture medium, adding DMEM / F12 culture medium containing exosome-free fetal bovine serum, verbascoside and lupeol for continuous culture; collecting cell culture supernatant, and after multiple centrifugation and microporous filter filtration, centrifuging to collect the precipitate, thereby obtaining extracellular vesicles rich in active mitochondria.The extracellular vesicles obtained by the preparation method of the application can significantly promote the proliferation of UVB-induced photoaging human skin fibroblasts, inhibit the expression of senescence-associated proteins p16 and p21, have the effects of repairing photoaging damage and reversing cell senescence, and have a broad application prospect.

The present application relates to the field of medicine, and more particularly to the use of aesculin or verbascoside in the preparation of a medicament for treating and / or preventing IgAN. The present inventors have found that aesculin and / or verbascoside can significantly improve proteinuria caused by IgAN and reduce inflammatory reactions, thereby playing a role in protecting kidney function, and do not have the serious shortcomings of clinically commonly used drugs, indicating that aesculin and / or verbascoside have a strong and low-toxicity effect in treating IgAN. In addition, aesculin and / or verbascoside are a natural compound, low toxicity and easy to be accepted by the human body.