The invention discloses a method for determining the content of beta-hydroxy-beta-methylbutyric acid in soybean
peptide protein powder. The method includes the steps that 1, during preparation of a sample solution, a sample is taken, 0.1 mol / L of a
hydrochloric acid solution is added to dissolve the sample, then the mixture is diluted to a scale with
acetonitrile, shaking and filtering, and subsequent filtrate is precisely sucked and diluted to a scale with 0.1 mol / L of a
hydrochloric acid solution to serve as a sample solution after uniform shaking; 2, during preparation of a comparison production solution, beta-hydroxyl-beta-
methyl butyrate hydrate is taken as a comparison product and diluted with 0.1 mol / L of a
hydrochloric acid solution, and the comparison product is prepared, wherein the content of beta-hydroxyl-beta-
methyl butyrate is 0.1 mg or 0.3 mg or 0.5 mg or 0.7 mg or 1.0 mg per ml of the comparison product; 3, during determining of the content of beta-hydroxyl-beta-
methyl butyrate, a
chromatographic column with octadecyl
silane silica being a filling agent is adopted, 0.01 mol / L of
sodium heptanesulfonate solution-
acetonitrile serves as a mobile phase, the target component is eluted and separated, and the content of beta-hydroxyl-beta-methyl
butyrate is calculated with a
linear regression equation. The method can accurately and effectively determine the content of beta-hydroxyl-beta-methyl
butyrate, and is simple and convenient to implement, economical, practical, good in
repeatability and high in accuracy.