Method for determining melamine content by using ultraviolet spectroscopy
A melamine and ultraviolet spectrum technology, applied in the measurement of color/spectral characteristics, etc., can solve the problems of complicated operation, low detection sensitivity, complex sample pretreatment, etc., and achieve the effect of accurate and effective measurement and simple operation.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] Establishment of the method for the interaction between melamine and DNA
[0020] (1) Use Tris (0.05mol / L)-HCl buffer solution with pH = 7.00 to prepare MM solution, DNA solution, and DNA-MM mixed solution respectively, shake well, let it stand for 20 minutes, and use the reagent blank as a reference to scan the electron absorption spectrum (i.e. ultraviolet spectrum);
[0021] (2) In a 1cm cuvette, add 3.00mL DNA solution, scan the electronic absorption spectrum or measure the absorbance by titration, add MM 10μL each time, and the volume effect can be ignored.
[0022] (3) Measure the electronic spectrum of DNA before and after adding MM to Tris(0.05mol / L)-HCl buffer solution with pH=7.00, see attached figure 1 . After the MM solution was added dropwise to the DNA solution, a small red shift (~2nm) and a color reduction effect (absorbance A decreased by ~0.05, ~10%) were observed at the position of the characteristic absorption peak of DNA at ~260nm, and the DNA - ...
Embodiment 2
[0025] Establishment of method for determination of melamine by ultraviolet spectrometry
[0026] In 3.00mL the concentration is 6.4×10 -5 A series of melamine solutions were added to the mol / L DNA solution so that the concentration of melamine was 0.084~1.68 μg / L. The absorbance of the DNA solution at 260 nm was measured before and after adding melamine. The data are listed in Table 1. Take the concentration of melamine as the abscissa, and take the difference (ΔA) between the absorbance of DNA without adding melamine and the absorbance of a DNA solution with a certain concentration of melamine as the ordinate to establish a working curve. The results are listed in the attached figure 2 . From the results, it can be seen that ΔA and the concentration of melamine conform to the Lambert-Beer law, showing a good linear relationship in the concentration range of 0.084~1.68μg / L; according to the definition of the international IUPCA: the detection limit is C DL =3S B / k, wh...
Embodiment 3
[0031] Detection of Melamine Residues in Feed Samples
[0032]Accurately weigh 5g of feed into a 50mL centrifuge tube. Add 20 mL of dimethyl sulfoxide (DMSO), shake for 1 hour, centrifuge to get 1 mL of supernatant, and prepare and dilute to 10 mL with Tris (0.05 mol / L)-HCl buffer solution for later use. Take 50.00μL of the above solution in 3.00mL DNA solution (concentration is 6.4×10 -5 mol / L DNA solution), shake well, stand still for 20 minutes, and measure its absorbance at 260nm. According to the established standard curve, calculate the residual content of melamine in the sample. The melamine residues in the 5 kinds of feed samples measured and the methods for the determination of melamine in the feed of the Ministry of Agriculture (NY1372-2007) (the minimum quantification limit of the HPLC method in this standard is 2.0mg / kg, and the minimum quantification limit of the GC-MS method is 0.05mg / kg) are listed in Table 2.
[0033] Table 2 Determination results of melami...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com