17 results about "DNA Solutions" patented technology

The invention relates to a preparation method of a microwave-assisted synthesized near-infrared electrochemical luminescence gold nanocluster and application of the gold nanocluster in miRNA (micro Ribonucleic Acid) detection. The preparation method comprises the following steps: under a stirring condition, adding a gold source and alkali into a Met solution to obtain a mixed solution, carrying out microwave irradiation reaction, and carrying out solid-liquid separation to obtain a Met-Au NCs solution; the preparation method comprises the following steps: activating a Met-Au NCs solution by using EDC and NHS, adding a DNA solution, mixing, and dialyzing to obtain a Met-Au NCs-DNA compound, namely the near-infrared electrochemical luminescence gold nanocluster. Compared with Au NCs prepared by a common mild method, the Met-stable Au NCs prepared by a microwave-assisted method has the advantage that the fluorescence and electrochemical luminescence efficiency at 835 nm is improved. The invention also provides a'signal closing 'ECL (electron cyclotron ligand) biosensor for high-sensitivity detection of the miRNA-107 (MicroRNA-107), and a method for detecting the miRNA-107 (MicroRNA-107). The sensor is simple to operate and good in repeatability, and has important scientific significance and application value for clinical early diagnosis of cancers.

Methods for obtaining a target median deoxyribonucleic acid (DNA) insert size utilizes several solid-phase reversible immobilization (SPRI) bead solutions. At different steps of the method workflows, different ratios of the SPRI bead solution to DNA solution are utilized. Each method includes back-to-back DNA fragment removal / retention steps, where different ratios of the SPRI bead solution to DNA solution are utilized in the back-to-back steps.

This invention provides a rapid hybridization reaction device for gene chips based on microfluidics, comprising a microfluidic layer based on microfluidic technology, a DNA probe microarray layer corresponding to the microfluidic channels in a dotted array, a temperature control module for providing the hybridization temperature between the DNA probes and DNA in the sample, and a fixing device that fixes and seals the microfluidic layer, DNA probe microarray layer, and temperature control module from top to bottom. Each microfluidic channel is in a continuous U-shaped configuration (positive-negative-positive-U), and is equipped with a liquid flow drive module for driving the DNA solution to flow back and forth within the microfluidic channel. After sealing, all probe array points are located within an independent microfluidic channel, and a sample dispensing orifice is provided at the upper end of the fixing device for adding samples to each independent microfluidic channel. Through the synergistic effect of temperature and flow rate, this device can reduce sample consumption, increase hybridization rate, reduce hybridization time, and avoid cross-contamination.

The application provides a method for identifying bases, drops a DNA solution on a molybdenum disulfide film to prepare a DNA testing device, uses a fluorescence spectrometer to detect fluorescence spectrum data of the molybdenum disulfide under the influence of bases, carries out characteristic matching degree comparison analysis based on the fluorescence spectrum curve of the molybdenum disulfide under the influence of unknown bases and the fluorescence spectrum curve of the molybdenum disulfide under the influence of known bases, and determines which kind of known base the unknown base is according to the matching degree comparison analysis result; the application can effectively identify bases by using the DNA and MoS2 adsorption and charge transfer mechanism, and has a lower requirement for the concentration of the used DNA solution to be tested; meanwhile, the MoS2 has the advantages of low cost and simple preparation, and compared with the prior art, the application has the advantages of low-concentration trace DNA base effective identification, simple device structure, simple operation and low cost.

The application belongs to the technical field of heparin extraction, and particularly relates to a method for determining the abundance of high anti-IIa activity heparin of pig small intestinal mucosa. The method comprises the following steps: (1) crushing fresh pig small intestinal mucosa; (2) using a PowerSoil kit to lyse the mucosa cells crushed in step (1), and performing water bath at 50-65 DEG C for 10-20 min, and then adding an elution buffer to elute the DNA; (3) using PCR to amplify the eluted DNA solution; (4) using a Nextera XT DNA library preparation kit to construct a library, and then using an Illumina NovaSeq 6000 sequencer to perform high-throughput sequencing; (5) using a DADA2 process to denoise and remove chimeric sequences, and using a 97% similarity threshold to cluster to generate OTU, and using a Greengenes database to annotate; (6) then using an LEfSe algorithm to screen differential flora, and using a random forest model to predict heparin activity.

This invention provides an application of DNA-modified polyacrylamide network-grafted liquid metal nanoparticles and / or polyacrylamide network-grafted liquid metal nanoparticles in ultra-rapid qPCR detection, relating to the field of molecular detection technology. The method includes the following steps: preparing DNA-modified polyacrylamide network-grafted liquid metal nanoparticles and / or polyacrylamide network-grafted liquid metal nanoparticles; adding a premix, fluorescent probe, primers, and liquid metal nanoparticles to a reaction vessel, followed by the addition of a target DNA solution to obtain the test solution; placing the test solution in an ultra-rapid real-time quantitative polymerase chain reaction (qPCR) platform to establish a fluorescence curve for the qPCR, and simultaneously establishing the detection linear range and detection limit. The provided method offers fast detection speed, high sensitivity, and strong specificity, with a detection time of less than 7 minutes.

This invention provides a method for preparing a DNA-chitosan composite modified layer, a zinc anode, and a zinc battery, comprising: 1) treating a zinc substrate to be coated; 2) preparing a DNA solution and a chitosan solution separately, adding the DNA solution to the chitosan solution and stirring to composite, followed by static curing and degassing treatment to obtain a DNA-chitosan composite spin-coating solution; the composition is adjustable by adjusting the total concentration of the spin-coating solution and the molar ratio of DNA to chitosan; 3) adding the composite spin-coating solution dropwise to the surface of the zinc substrate to spin-coat a film, and obtaining a zinc anode with a DNA-chitosan composite modified layer on the surface after drying and curing. This invention utilizes the abundant nitrogen-containing bases and phosphate groups in DNA to provide zinc-loving sites, which, in synergy with the film-forming properties and amino coordination of chitosan, guide the uniform deposition of zinc ions, inhibit side reactions such as dendrite formation, hydrogen evolution, and corrosion, and significantly improve the coulombic efficiency and cycle life of aqueous zinc-ion batteries.

The invention belongs to the technical field of detection, and particularly relates to a silver nanocluster and a preparation method and application thereof. The preparation method of the silver nano-cluster comprises the following steps: (1) mixing and incubating a denatured DNA solution, a silver ion solution and a reducing agent to obtain the silver nano-cluster; wherein the DNA is a three-chain DNA, the three-chain DNA is formed by embedding a double-chain DNA into a third DNA chain through a Hoogsteen hydrogen bond or an anti-Hoogsteen hydrogen bond, the nucleotide sequence of the double-chain DNA is as shown in SEQ ID NO.1, and the nucleotide sequence of the third DNA chain is as shown in any one of SEQ ID NO.2 to SEQ ID NO.7. The invention also discloses a preparation method of the DNA. The silver nanocluster is excellent in stability and high in quantum yield; and when the silver nanocluster is used for detecting miRNA, the sensitivity and the specificity are high, and the correlation between the detection result of a clinical sample and the detection result of gold standard RT-qPCR is high.

The application belongs to the field of bioelectrochemical sensors, and discloses a biosensor and a preparation method and application thereof. The preparation method is as follows: gold source solution is used as an electrolyte, an electrode part (including a working electrode, a reference electrode and a counter electrode) is placed in the electrolyte to perform electrochemical deposition, thereby uniformly depositing gold nanoparticles on the surface of the working electrode to prepare a gold nanoparticle modified working electrode; the deposition voltage is 0.4-0.6 V; a thiol-modified redox mediator-single-stranded DNA solution is added dropwise on the surface of the gold nanoparticle modified working electrode, and after incubation, the biosensor is prepared. The method can complete the direct deposition of gold nanoparticles on the surface of the working electrode through one-step operation, is simple and efficient, is conducive to batch production and application, and can be used for the detection of target nucleic acids.

This invention discloses a rapid quantitative detection method for Salmonella using LFS-SERS, belonging to the field of food safety technology. The method includes the following steps: S1, lysing the sample to obtain a crude DNA solution; S2, using the crude DNA solution as a template, performing Salmonella-specific LAMP amplification to obtain a biotin-labeled product; S3, mixing dCas9 protein, sgRNA, SERS-labeled gold nanoparticle-probe conjugate, the biotin-labeled product, and a loading buffer solution, incubating at room temperature for 1-5 minutes, performing a colorimetric reaction using a lateral flow test strip, and using a portable Raman spectrometer connected to a smart terminal to detect the T line in the LFS using SERS, thus achieving quantitative detection of Salmonella in the sample. This method is highly specific, sensitive, and stable; the entire detection process can be completed within 40 minutes, and it is low-cost and easy to operate.

The invention discloses a preparation method and application of a novel azobenzene modified nanopore probe, and belongs to the technical field of biological analysis and detection. The preparation method comprises the following steps: dissolving DNAs modified by dibenzocyclooctyne in water to obtain a DBCO-DNA solution; adding a buffer solution into the DBCO-DNA solution, then adding an azide-polyethylene glycol (5)-N-hydroxysuccinimide ester aqueous solution to carry out a click chemical reaction, and purifying to obtain an intermediate product; and adding a 4-aminoazobenzene hydrochloride acetonitrile solution into the intermediate product, and carrying out a coupling reaction under an alkaline condition to obtain the product. The invention also discloses a novel azobenzene modified nanopore probe and application thereof. The novel azobenzene modified nanopore probe can generate nanopore characteristic signals with characteristics and distinguishability, real-time, label-free, damage-free and high-sensitivity detection on enzymes and small molecules can be achieved by combining the azobenzene modified nanopore probe with a nanopore single-molecule detection platform, and the application potential is large.

The invention discloses a DNA (deoxyribonucleic acid) gel dish desalting treatment method and application, which is characterized in that a DNA solution is dialyzed and replaced through a natural molecular sieve of gel by utilizing the molecular sieve effect formed by agarose gel polymerization and combining the characteristic that DNA is a long-chain macromolecule, so that the DNA treated by sodium bisulfite is desalted and purified. Meanwhile, the molecular sieve of the gel is used for dialyzing the desalted solution, the tedious process of removing hydrosulphite and re-purifying DNA in DNA methylation sequencing is innovatively solved, the treated long-chain DNA is wrapped in agarose gel, the loss or loss of DNA in the dialysis process is small, an expensive purification reagent and a purification step with tedious operation are not needed, and the method is suitable for large-scale industrial production. The experiment cost and the operation process can be greatly saved.

The invention discloses a method for accelerating DNA strand displacement reaction by using n-butyl alcohol, and belongs to the technical field of nucleic acid reaction. The method comprises the following steps: providing a water-phase DNA solution containing an input chain and double-chain compound; under proper conditions, the aqueous-phase DNA solution is in full contact with n-butyl alcohol, so that an input chain and a bottom chain of the double-chain compound are subjected to complementary pairing, a top chain is released, and the DNA chain replacement reaction is completed. Instantaneous phase separation and dehydration concentration of a water phase / an organic phase are carried out on a system by utilizing n-butyl alcohol, and the effective concentration and the molecular collision frequency of DNA molecules are instantaneously improved, so that the reaction rate of DNA strand displacement is remarkably accelerated. The method can provide a new strategy for DNA calculation and acceleration of a nucleic acid molecule logic circuit.

The application relates to the technical field of gene SNP molecular markers, in particular to a gene SNP molecular marker method and system for watermelon breeding, which comprises the following steps: extracting DNA solutions of watermelon samples of multiple varieties respectively, and performing SNP typing-based fingerprint construction; obtaining 16S rRNA sequences of watermelon microbial communities, dividing the 16S rRNA sequences into base sequences; determining mutation correlation parameters of the bases; determining a transition probability matrix of the 16S rRNA sequences; correcting sampling step lengths of the 16S rRNA sequences; correcting the transition probability matrix; determining characteristic vectors of the 16S rRNA sequences; determining genetic distances between the 16S rRNA sequences of the watermelon microbial communities and soil samples, constructing a phylogenetic tree, and determining strongly correlated microbial communities of the breeding environment of the watermelon samples of the multiple varieties. The adaptability of the watermelon breeding environment is improved.

The invention discloses lipophilic modification and biological application of G-base-rich single-stranded DNA (deoxyribonucleic acid). The lipophilic G-base-rich single-stranded DNA is obtained by taking the G-base-rich single-stranded DNA as an element and carrying out molecular modification on a phosphoric acid skeleton or a base of the single-stranded DNA by adopting a lipophilic group and a fluorophore. The modified lipophilic fluorescent G-base-rich single-stranded DNA molecules are dissolved in a Tris buffer solution, a lipophilic fluorescent single-stranded DNA solution is obtained through thermal denaturation and renaturation treatment, and the DNA solution can serve as a cell membrane staining agent to be applied to cell morphology and structure research, cell morphology and structure research or positioning and detection. The single-stranded DNA rich in G basic groups is used as a carrier, so that the biosafety is good, the structure is stable, the synthesis is easy, and the biocompatibility and affinity are very good. Meanwhile, the preparation is simple, the structure is stable, no toxic or side effect exists, and the dye can be used as a novel membrane dye reagent of a biological membrane or a fat-soluble biological structure membrane.

The invention discloses a host DNA removal method based on magnetic separation. According to the method, human cells in a to-be-detected sample are specifically adsorbed through streptavidin magnetic beads, complete cells in the sample are pulled out, free DNA in the sample is removed through nuclease, a purified microorganism DNA solution is obtained, and finally genome DNA is extracted and can be used for related sequencing and research. According to the DNA solution obtained by the method disclosed by the invention, the content of host DNA is greatly reduced, so that the sequencing accuracy is improved.

The invention discloses hydrogel based on biomass DNA and a preparation method of the hydrogel. The hydrogel based on the biomass DNA comprises a biomass DNA solution and a phase separation dehydrating agent, the concentration of the biomass DNA in the biomass DNA solution is greater than the chain entanglement concentration of the biomass DNA in the biomass DNA solution; the phase separation dehydrating agent is a reagent which is subjected to phase separation with the biomass DNA solution and induces dehydration of the biomass DNA solution. The technical problem that existing DNA hydrogel is weak in mechanical property is solved, and the prepared hydrogel shows excellent mechanical property.