Three-branch-like nucleic acid nano silver fluorescent cluster as well as preparation method and application

A nucleic acid nano and fluorophore technology, applied in the field of biofunctional fluorescent nanomaterials, can solve problems such as the inability to regulate the performance of nanosilver clusters, achieve strong fluorescence effects, improve sensitivity, and expand the application range
CN108359448AInactive Publication Date: 2018-08-03TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2018-08-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a three-branch-like nucleic acid nano silver fluorescent cluster as well as a preparation method and an application. The preparation method comprises the steps as follows: (1)three oligonucleotides are designed and synthesized according to a base complementary pairing principle to be used for forming three-branch-like DNA with the same sticky ends on all branches; (2) thethree oligonucleotides are prepared into corresponding aqueous solutions; (3) a three-branch-like DNA solution with the same sticky ends on all branches is prepared; (4) the three-branch-like DNA solution with the same sticky ends on all branches, a silver nitrate aqueous solution, a phosphate buffered solution and a sodium borohydride aqueous solution are stirred, and the three-branch-like nucleic acid nano silver fluorescent cluster is obtained. The three-branch-like nucleic acid nano silver fluorescent cluster is stronger in fluorescent effect and stable, the sensitivity of a fluorescent material in analysis and detection is improved, and the application range of the nucleic acid nano silver fluorescent material is expanded. The nucleic acid nano silver fluorescent cluster can detect Dtype amino acid.
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Description

technical field

[0001] The invention belongs to the technical field of biological functional fluorescent nanomaterials, and in particular relates to a three-dendritic nucleic acid nano-silver fluorescent cluster and a preparation method thereof. Background technique

[0002] With the continuous development of nanoscience and biotechnology, the application of nanofluorescent materials in environmental detection, biometric detection, bioimaging labeling, biocatalysis and other fields has become more and more extensive, which has attracted great attention from governments and academic circles around the world.

[0003] Traditional D-amino acid analysis and detection methods include optical analysis, electrochemical analysis, nuclear analysis, chromatographic analysis, and electron microscope analysis. The above method has been used for the detection of target objects, with low detection line and good repeatability, but it needs to rely on large-scale analytical instruments and ...

Claims

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