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Multi-branched nucleic acid nano-silver fluorescent cluster and preparation method and purpose thereof

A nucleic acid nanometer and fluorophore technology, applied in the field of biological functional fluorescent nanomaterials, can solve the problems of inability to control the performance of nanometer silver clusters, and achieve the effect of strong fluorescence effect.

Inactive Publication Date: 2018-09-04
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nucleic acid has good biocompatibility, but current research only uses single-stranded nucleic acid to synthesize silver nanomaterials, and cannot regulate the properties of silver nanoclusters, such as the regulation of stability and fluorescence.

Method used

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  • Multi-branched nucleic acid nano-silver fluorescent cluster and preparation method and purpose thereof
  • Multi-branched nucleic acid nano-silver fluorescent cluster and preparation method and purpose thereof
  • Multi-branched nucleic acid nano-silver fluorescent cluster and preparation method and purpose thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation method of the first polydendron nucleic acid nano-silver fluorophore cluster comprises the following steps:

[0046] (1) According to the principle of complementary base pairing, design and synthesize three oligonucleotides, and name these three oligonucleotides as A 1 、A 2 and A 3 , used to form three branches of DNA each with the same cohesive ends;

[0047] The sequence consisting of 22 nucleotides of each sequence of the tridendritic oligonucleotide is a cohesive end;

[0048] A 1 、A 2 and A 3 The nucleotide sequences are shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3, respectively.

[0049] SEQ ID NO.1:

[0050]

[0051] SEQ ID NO.2:

[0052]

[0053] SEQ ID NO.3:

[0054]

[0055] The sequence consisting of 22 nucleotides of the 5' of the above three sequences is a cohesive end;

[0056] The sequence (marked with dotted line) formed by the 23rd-36 nucleotides of the SEQ ID NO.1 sequence is reverse complementary to the sequence...

Embodiment 2

[0063] The preparation method of the first polydendron nucleic acid nano-silver fluorophore cluster comprises the following steps:

[0064] (1) with embodiment 1 step (1);

[0065] (2) with embodiment 1 step (2);

[0066] (3) Take the same volume of A with a volume of 20 μL 1 Aqueous solution, A 2 aqueous solution and A 3 Aqueous solution and 10 μL of 200 mM sodium chloride aqueous solution, supplemented with water to 100 μL, mixed evenly, heated to 90 ° C, cooled to room temperature, to obtain a three-dendritic DNA solution with the same sticky end in each branch;

[0067] (4) Take the three-dendritic DNA solution and the 3mM silver nitrate aqueous solution that each branch has the same sticky end, mix well, add the phosphate buffer solution of pH=6, stir for 30min, add the 3mM sodium borohydride aqueous solution, stir for 1h, Obtain the first multi-dendritic nucleic acid nano-silver fluorescent cluster;

[0068] The molar ratio of the three-branch DNA, silver nitrate an...

Embodiment 3

[0070] The preparation method of the first polydendron nucleic acid nano-silver fluorophore cluster comprises the following steps:

[0071] (1) with embodiment 1 step (1);

[0072] (2) with embodiment 1 step (2);

[0073] (3) Take the same volume of A with a volume of 30 μL 1 Aqueous solution, A 2 aqueous solution and A 3 Mix the aqueous solution with 10 μL of 200 mM sodium chloride aqueous solution, heat to 95°C, and cool to room temperature to obtain a three-dendritic DNA solution with the same sticky ends on each branch;

[0074] (4) Take the tridendritic DNA solution and 5mM silver nitrate aqueous solution that each branch has the same sticky end, mix well, add a phosphate buffer solution with pH=8, stir for 60min, add 5mM sodium borohydride aqueous solution, stir for 2h, Obtain the first multi-dendritic nucleic acid nano-silver fluorescent cluster;

[0075] The molar ratio of the three-branch DNA, silver nitrate and sodium borohydride that each branch has the same st...

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Abstract

The invention discloses a multi-branched nucleic acid nano-silver fluorescent cluster and a preparation method and a purpose thereof. The preparation method comprises the following steps: 1) accordingto a complementary base pairing principle, designing to synthesize three oligonucleotides to form three-branched DNA with each branch having a same cohesive end; 2) preparing three oligonucleotides to a corresponding aqueous solution; 3) preparing a three-branched DNA solution with each branch having the same cohesive end; and 4) taking the three-branched DNA solution with each branch having thesame cohesive end, a silver nitrate aqueous solution, a phosphatic buffer solution, and a sodium borohydride aqueous solution for stirring to obtain the multi-branched nucleic acid nano-silver fluorescent cluster. The multi-branched nucleic acid nano-silver fluorescent cluster has strong fluorescence effect, and is stable, the cohesive end can be changed, and the multi-branched nucleic acid nano-silver fluorescent cluster endows the novel applications to a material by connecting different functional molecules. By using the silver particles in the material, the multi-branched nucleic acid nano-silver fluorescent cluster has inhibiting effect for Gram-negative bacteria positive bacterium and Gram-negative bacteria.

Description

technical field [0001] The invention belongs to the technical field of biological functional fluorescent nanometer materials, and in particular relates to multi-branched nucleic acid nanometer silver fluorescent clusters and a preparation method thereof. Background technique [0002] With the continuous development of nanoscience and biotechnology, the application of nanofluorescent materials in environmental detection, biometric detection, bioimaging labeling, biocatalysis and other fields has become more and more extensive, which has attracted great attention from governments and academic circles around the world. [0003] Traditional D-amino acid analysis and detection methods include optical analysis, electrochemical analysis, nuclear analysis, chromatographic analysis, and electron microscope analysis. The above method has been used for the detection of target objects, with low detection line and good repeatability, but it needs to rely on large-scale analytical instrum...

Claims

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Application Information

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IPC IPC(8): C09K11/58A01N59/16A01P1/00B82Y20/00B82Y40/00
CPCA01N59/16B82Y20/00B82Y40/00C09K11/58
Inventor 仰大勇杨璐
Owner TIANJIN UNIV
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