351 results about "Luminol" patented technology

A multi-zonal system and method for detecting contraband substances associated with a human subject. Objects containing such substances may be carried by the subject or in a garment worn by the subject, or as vapors entrained by the subject's garments. Alternatively, the substance may be present as small particles or residues present on skin, garment fibers, or the like that may be dislodged from the subject or his immediate effects. The system comprises: (i) an examination station through which the subject passes; (ii) sample generation means to produce a sample for analysis, comprising a plurality of air jets disposed in the examination station to impinge flowing gas onto pre-selected zones on the subject; (iii) collection means operative to acquire and convey the sample, comprising a plurality of collection ports disposed in the examination station to receive gas deflected by the subject from the air jets; (iv) analysis means in communication with the collection means to receive the sample and carry out a chemical analysis to detect the contraband substances; and (v) signal means operably connected to the analysis means for indicating detection of the contraband substances. Preferably the system employs a chemiluminescent detector based on the reaction of luminol with NO₂ produced by pyrolysis of explosive or other contraband substance and is capable of high throughput, accurate screening in real time of a large number of subjects, e.g. persons being screened in an airport.

The invention discloses an application of nano-gold directly bonded with luminol in an immunoassay. The invention is characterized in that the immunoassay probe of a nano-gold directly bonded with luminol comprises antibodies which are labeled by the nano-golds directly bonded with luminol, wherein the nano-gold directly bonded with luminol are prepared through reducing chloroauric acid by the luminol at a single step; a chemiluminescence immunoassay method is based on the immunoassay probe of the nano-gold directly bonded with luminol; and a kit is used for carrying out the immunoassay method. The chemiluminescence immunoassay method of the invention has the advantages of high sensitivity (for instance, the detection limit can reach 1.0pg/mL for detecting human IgG), wide range of linearity, good repeatability, simple operation, low cost and the like, can be applied to detect multi-analyte in various samples and has key application prospect in fields, such as clinical diagnosis and cure, drug analysis, food security detection, environmental monitoring and the like.

The invention provides preparation and application of an alpha fetoprotein and carcino-embryonic antigen electrochemiluminescence sensor, and belongs to the technical fields of nano function material, clinical analysis, a bioseneor technology and electrochemistry. The characteristics that platinum nanoparticle @meso-porous silicon @ graphene nanocomposite (PtNPs@m-Si@GS) is strong in conductivity, good in stability, large in specific surface area, good in biocompatibility, strong in catalytic activity and the like are utilized in preparation; an alpha fetoprotein second antibody (anti-alpha fetoprotein (AFP)) and a carcino-embryonic antigen secondary antibody (anti-carcino-embryonicantigen (CEA)) are marked, so as to prepare the marked second antibodies Ru-PtNPs@M-Si@GS/anti-AFP and luminol-PtNPs@M-Si@GS/anti-CEA; and the sensitivity of the sensor is obviously improved. Compared with other single-channel electrode sensors, alpha fetoprotein and carcino-embryonic antigen can be simultaneously detected on a same electrode at one time; the detection efficiency is obviously improved; and the alpha fetoprotein and carcino-embryonic antigen electrochemiluminescence sensor has important scientific significance and application value on clinical early diagnosis of hepatic carcinoma.

The invention discloses a preparation method of an unmarked electrochemiluminescent tumor marker immunosensor and application of the immunosensor in detection of tumor markers, and belongs to the field of novel nano functional materials and biosensors. According to the preparation method of the unmarked electrochemiluminescent tumor marker immunosensor, a novel micro-nano material NH4CoPO4 is taken as a substrate so that the luminescence stability of a luminol-hydrogen peroxide luminescent system can be greatly enhanced, and meanwhile, rod-like gold@siver core-shell nanoparticles are taken as a biomimetic catalytic luminescent system, and therefore, an unmarked electrochemiluminescent immunosensor, which is low in cost, high in sensitivity, good in specificity, fast in detection and simple to prepare and is used for detecting tumor makers, can be prepared.

The invention discloses a luminol direct bonded nano gold nucleic acid analyzing probe and a novel chemiluminescence analysis method. The invention provides the luminol direct bonded nano gold nucleic acid analyzing probe, a chemiluminescence analysis method based on the luminol direct bonded nano gold nucleic acid analyzing probe and a kit for constructing the analyzing method; the analyzing probe comprises nucleic acid which is directly bonded by luminol and marked by nano gold; and luminol direct bonded nano gold is obtained by reducing chloroauric acid at one step by luminol. The chemiluminescence analysis method based on the nucleic acid analyzing probe of the invention has the advantages of high sensitivity (for example, specific sequence single-chain DNA detection limit can reach 1.9*10<-16>mol/L), wide linear range, high repeatability, simple operation, low cost, and the like, can be used for the detection of DNA, RNA and adapter corresponding ligands in various samples and has wide application prospect in the fields of clinical diagnosis and treatment, pharmaceutical analysis, food safety detection, environmental monitoring, and the like.

The invention belongs to the field of chemiluminescence sensors, and relates to a method for determining melamine (Me), which is implemented by using a target induced chain releasing technique and combining with a hybrid chain reaction and a chemiluminescence technique. When a target Me exists, the Me acts with aptamer DNA1 (deoxyribonucleic acid 1) in double-stranded DNA on the surface of a functionalized magnetic bead (FMB1) so as to release a complementary sequence DNA2 of the aptamer; and the released complementary sequence DNA2 acts with another functionalized magnetic bead (FMB2), and a chain hybridization reaction is performed in the presence of hairpin DNA (HRP-H1 and HRP-H2) for modifying HRP, so that the two kinds of modified DNA grow into long double-stranded DNA. Through magnetic separation, and by using the catalytic action of HRP on a luminol-H2O2 chemiluminescence system, chemiluminescence is produced, and then the determination of the Me is realized through determining the chemiluminescence intensity. The method is high in selectivity and detection sensitivity.

The invention relates to a diagnostic reagent kit for testing the hepatitis c virus (HCV) and the preparation and test method, which is to add the HCV recombinant antigen used for peridium into the buffer solution, blend it, move into the luminous microplate, make incubation for 18 hours under 4DEG.C, wash the luminous microplate, add into the confining liquid, leave the liquid after incubation and fully dry the luminous microplate to complete the preparation of the pre-peridium luminous microplate; combine the anti-human IgG used for marking and the horse radish peroxidase by improving the sodium periodate to complete the preparation of the enzyme marker; prepare the chemical luminous substrate solution A with luminal, Tween20 and luminous intensifier and prepare the chemical luminous substrate solution B with the hydrogen peroxide. The reagent kit also comprises the sample diluent and concentrated scrub solution. The negative corresponds to the normal human serum while the positive corresponds to the people with serum of pooled serum with HCV antibody. The reagent kit provided in the invention has much higher detection sensitivity than the ELISA, which is safe and reliable, easy to operate with low cost, and without any expensive full-automatic chemical luminous measuring apparatus required.

The invention provides a chemiluminescent substrate solution, which comprises the following components: 0.2-1.2g/L of Luminol, 0.1-5g/L of cinnamic acid, 0.05-0.6g/L of 4-iodophenylboronic acid, 0.05-0.6 g/L of p-iodophenol, 5-50ml/L of dimethylformamide (DMF), 2.5-7.5 g/L of polyvinyl alcohol (PVA), 5-15g/L of polyvinyl pyrrolidone (PVP), 1.5-5g/L of polyethylene glycol 600 (PEC -600), 1-10g/L of ethylene diamine tetraacetic acid (EDTA), 400,000-4,000,000 unit/L of gentamicin sulfate, 0.1-1.0g/L of carbamide peroxide and 0.1mol/L of tris buffer solution with a pH vale being 9.0. The chemiluminescent substrate solution is a solution which can be directly used, and maintains wide linear detection range, good stability and high sensitivity, thus solving the problem that a traditional chemiluminescent substrate solution consists of a solution A and a solution B which are stored separately; and after the solution A and the solution B are isovolumetrically mixed for 10 minutes, signal values are decreased sharply, the detection range is narrowed and the stability is poor. In addition, the chemiluminescent substrate solution provided by the invention is easy to prepare, low in cost and simple to operate, environmentally friendly, and non-toxic and harmless to human bodies and the like.

The invention discloses a photodynamic treatment medical composition which consists of a photosensitizer and a chemical activator, wherein the photosensitizer is expressed by the formula I; the chemical activator comprises luminol, p-iodophenol, horse radish peroxidase and hydrogen peroxide; and the proportion of the photosensitizer expressed by the formula I, to the luminol to the p-iodophenol to the horse radish peroxidase to the hydrogen peroxide in the composition is 8-12muM:0.4-0.7mM:1-1.5mM:0.01-0.02mg/mL:2-3mM. In the composition of the invention, active oxygen generated by bioluminescence energy transfer between the photosensitizer expressed by the formula I and the luminol is utilized to kill cells and fungi. Compared with the traditional photodynamic treatment method, the composition can be used for treating in deep tissues; and damages to normal tissues, which are caused by long-time irradiation by an external light source, can be avoided; and meanwhile, the composition has a profound influence on the aspects of the clinic treatment of tumors and pathogenic bacteria infection.

Provided is luminescent gold nanomaterial functionalized by N-(4-aminobutyl)-N-ethylisoluminol, methods of preparation and application thereof. The functionalized gold nanoparticle nanomaterial are formed by N-(4-aminobutyl)-N-ethylisoluminol bonding to the surface of the gold nanomaterial. The functionalized gold nanomaterial are prepared by directly reducing chloroauric acid with N-(4-aminobutyl)-N-ethylisoluminol, wherein N-(4-aminobutyl)-N-ethylisoluminol acts as reducer and stabilizer simultaneously. The preparation method is simple, fast and no need of special conditions. The preparation methods can be performed in a wide temperature range, for example, 15-35° C. The size and pattern of the functionalized gold nanomaterial can be specified by choosing the ratio of chloroauric acid to N-(4-aminobutyl)-N-ethylisoluminol. The obtained functionalized nano gold particles exhibit excellent chemiluminescence properties. Said functionalized gold nanomaterial can be combined with biomolecules to form biomolecular probe, which can be used for immunoassay, nucleic acid analysis, molecular imaging, sensor, etc., and has a broad application prospect in the field of clinical analysis biomedicine, food safety, and environment monitoring.

The invention discloses a biological sensor based on CuS nano-particle cation exchange enhanced chemiluminescence. Copper sulphide nano-particles are marked on biological molecules, and a composite structure marked with copper sulphide is obtained through a biological identification function; silver nitrate is added into a system, cation exchange reaction on silver nitrate and copper sulfide nano-particles is instantly carried out so as to generate copper ions; a target is quantitatively detected by utilizing chemiluminescence caused by catalyzing a luminol-hydrogen oxide system by the generated copper ions.

The invention relates to an O-type foot-and-mouth disease virus antibody chemiluminescence detection kit. The kit includes an polystyrene board coated by antigen, a monoclonal antibody, a standard substance, a luminescence substrate solution A and a luminescence substrate solution B. The polystyrene board coated by antigen is an opaque polystyrene plate coated by an O-type foot-and-mouth disease virus RE2 recombinant protein; the monoclonal antibody is a horseradish peroxidase labeled O-type foot-and-mouth virus monoclonal antibody; the luminescence substrate solution A comprises a Lumino, hydroxycoumarin, gallic acid, a Tris-Hcl buffer and water; the standard substance is foot-and-mouth virus O-type antibody respectively diluted buy a calibration diluent, wherein the dilution is 0NU / mL, 2NU / mL, 5NU / mL, 10NU / mL, 30NU / mL and 60NU/ mL respectively. The kit of the invention can be used for detecting O-type foot-and-mouth disease virus antibody, has the advantages of high sensitivity and wide detection range, and realizes the quantitative detection of foot-and-mouth disease antibody.

The invention belongs to the technical field of analytical chemistry and optical DNA biosensing and relates to a method for detecting Hg<2+> with a signal-off chemiluminescence method. The method comprises the following steps: a mispairing hairpin DNA sequence containing a G-DNA structure fragment and part of T-T is adopted; when K<+> and hemin exist, the hairpin DNA configuration is changed to form a quadruplex G-DNA structure with peroxidase catalytic activity, and the structure can catalyze hydrogen peroxide oxidation luminol to generate chemiluminiscence; when Hg<2+> exists in a system, as the hairpin DNA structure is stabilized through the formation of a T-Hg-T structure, the quadruplex G-DNA structure cannot be formed, and chemiluminiscence is quenched; according to the influence of the change of concentrationof Hg<2+> on the quenching degree of chemiluminiscence, the detection on Hg<2+> is realized. The optical DNA biosensing method has the advantages of easiness in operation, short response time, good stability, high flexibility and high selectivity.