53 results about "Luminol" patented technology

The application discloses an electrochemiluminescence immunosensor for detecting interleukin-1 beta and a preparation method and application thereof, and comprises a three-electrode system of a working electrode, a reference electrode and a counter electrode; the working electrode is a modified electrode of CoAl layered double hydroxide, is loaded with platinum nanoparticles after being etched by alkali, and is obtained by fixing interleukin-1 beta antibodies and blocking non-specific sites; and a solution containing luminol is used as an electrolyte during detection of the immunosensor. The working electrode of the immunosensor takes oxygen-rich vacancy CoAl layered double hydroxide as a substrate, combines with platinum nanoparticles to realize EMSI effect, significantly enhances the electrochemiluminescence signal of a luminol-dissolved oxygen system, realizes quantitative detection of IL-1 beta by combining with specific antigen-antibody recognition, and has the characteristics of strong anti-interference ability, fast detection speed and good stability.

The invention discloses an immunofluorescence imaging method for driving tyramine signal amplification based on chemiluminescence and application thereof, and relates to the technical field of biomedical imaging. The immunofluorescence imaging method comprises the following steps: carrying out first incubation on a to-be-detected sample and a peroxidase-labeled antibody; performing second incubation on the to-be-detected sample after the first incubation and a tyramine substrate containing a fluorophore under a dark condition; adding a chemiluminescent substrate solution into the to-be-detected sample after the second incubation, and carrying out image acquisition; wherein the antibody can be specifically bound with a target protein; the tyramine substrate can generate active free radicals under the catalysis of peroxidase, so that the active free radicals are covalently cross-linked with tyrosine residues; the chemiluminescent substrate solution comprises hydrogen peroxide and luminol. The immunofluorescence imaging method improves imaging signal and fluorescence stability, effectively reduces background interference and phototoxicity, is suitable for long-time stable fluorescence immunoimaging, and is especially suitable for high-sensitivity detection of low-expression protein markers.

The application relates to a method for enhancing electrochemiluminescence signals by ion-pi interaction, belonging to the fields of electrochemiluminescence and analytical chemistry. 2+ By modifying the working electrode with a conjugated pi system, cation-pi interaction is introduced into the Ru(bpy)3 / triethylamine (TEA) system, efficient enrichment of TEA protonated cations near the electrode is realized, thereby greatly enhancing the electrochemical reaction rate and ECL signal. Further, anion-pi interaction is utilized to realize ECL signal enhancement of the luminol ECL system. In addition, the conjugated pi system is exposed to the outermost boundary of the electrode, so as to provide effective ion-pi interaction for ECL; when the pi system is covered by an alkyl chain, the modified electrode can only exhibit hydrophobic interaction. The method disclosed by the application provides a new strategy for extending ion-pi interaction to all types of ECL systems and constructing a high-sensitivity ECL analysis platform.

This invention relates to a dual-potential electrochemiluminescence immunosensor based on enzymatic in-situ generation of a co-reactant, its preparation method, and its application. The sensor includes: a substrate electrode; a GOx@Au@MoS2 multifunctional sensing interface; a hybrid probe consisting of a cathode probe and an anode probe; the cathode probe is a Py-HOF-labeled AFB1 antibody, and the anode probe is a UiO66-NH2@luminol-labeled OTA antibody; glucose is added to the detection solution, and hydrogen peroxide is generated in situ through immobilized glucose oxidase, serving as the single co-reactant for both the cathode and anode. During detection, this invention generates H2O2 in situ through enzymatic generation, simultaneously exciting ECL signals at both the cathode and anode; it solves problems such as poor stability of the H2O2 co-reactant, inconvenient concentration control, multiple operation steps, and insufficient continuous supply; and it enables simultaneous quantitative detection of AFB1 and OTA with a single sample addition, without the need for external H2O2.

The invention relates to the technical field of electrochemical luminescence sensing, and discloses an electrochemical luminescence immunosensor for detecting interleukin-1 beta, a preparation method and application thereof, the electrochemical luminescence immunosensor comprises a working electrode, a reference electrode and a counter electrode; the working electrode comprises a substrate electrode and an ordered mesoporous silica film (SNF) modified on the surface of the substrate electrode; a composite nano-enzyme containing cobaltosic oxide nanoparticles and nitrogen-doped graphene quantum dots is loaded in a nano-pore channel of the SNF, the outer surface of the SNF is modified with an epoxy group, and an anti-interleukin-1beta antibody is covalently immobilized through the epoxy group; a detection system of the electrochemical luminescence immunosensor is a luminol / dissolved oxygen system. According to the electrochemical luminescence immunosensor, a luminol / dissolved oxygen system signal can be remarkably enhanced, and high-sensitivity and accurate quantitative detection of interleukin-1 beta is realized under a neutral condition.

This invention belongs to the field of immunoassay and biosensing technology, and discloses a method for preparing a ratiometric ECL biosensor based on Eu / Tb-Lu MOGs. Using Eu / Tb-Lu MOGs as the luminescent material and H3-tctpy and luminol as ligands, a single-luminescent material with dual ECL emission is synthesized. Lanthanide ions provide the cathode ECL emission, enhanced by the antenna effect and Tb→Eu energy transfer; the anode emits light from luminol using dissolved oxygen in water, requiring only the addition of K2S2O8 as a single co-reactant, resulting in fewer side reactions and a stable signal. The dual-potential ratiometric sensing utilizes the dual ECL signal for self-calibration, improving anti-interference and reliability; the single-luminescent dual-potential system achieves synchronous anode / cathode luminescence, simplifying the system and improving detection accuracy. By detecting changes in the ECL signal of different concentrations of analytes, the detection of microRNA-126 is achieved.

This invention develops a pH-responsive controlled-release electrochemiluminescence sensor based on glucose oxidation and its application in the detection of CYFRA 21-1, belonging to the field of electrochemiluminescence sensor construction technology. The sensor constructed in this invention uses BSA / luminol-Ab2 / SiO2-PEI@Au NPs as the secondary antibody label and CeO2-Au as the substrate material. It utilizes the oxidation of glucose to gluconic acid under voltage, thereby lowering the pH. The sensor constructed in this invention has a wide detection range, high sensitivity, and low detection limit, providing a feasible solution for the detection of CYFRA 21-1.

The application belongs to the technical field of electroluminescence, and particularly relates to a preparation method of a high-entropy oxide material and application of the high-entropy oxide material in electrochemiluminescence detection of levofloxacin. The high-entropy oxide material is a kind of five-element high-entropy oxide HEO-800 material with simple raw materials, convenient synthesis, excellent electrochemiluminescence performance, which is synthesized by taking metal nitrate of Fe, Co, Cu, Zn and Ni as raw materials, and taking Na2CO3 and NaOH as raw materials. The HEO-800 material has excellent conductivity and chemical stability, and a multi-electron reaction potential accelerates the conversion of dissolved oxygen into reactive oxygen species (ROS), enhances the electroluminescence intensity, and based on the quenching effect of LVX, a method for detecting LVX by using a luminol / HEO-800 system is established. By fitting the change of ECL intensity with the concentration of LVX, a linear calibration curve is obtained, the correlation coefficient is 0.993, and the detection limit is 1.02*10 ‑5 μM (S / N=3). The material has good sensitivity, stability and reproducibility for electrochemiluminescence detection of LVX, and provides a new scheme for designing a new electrocatalyst for testing LVX.

The invention discloses an HRP chemiluminescent substrate system, a kit and a preparation method, the HRP chemiluminescent substrate system is composed of a liquid A and a liquid B which are physically isolated and stored; the solution A is an alkaline buffer system with the pH value of 9.0-9.6, and comprises isoluminol or a salt substrate thereof, a cationic surface active agent CTAC and a metal chelating agent diethylene triamine pentaacetic acid; and the solution B is an acidic solution with the pH value of 4.5-5.5, and comprises an oxidizing agent and a reinforcing agent N-(3-chloro-4-hydroxyphenyl) acetanilide. According to the invention, the reinforcing agent N-(3-chloro-4-hydroxyphenyl) acetanilide is placed in the acidic liquid B, when the acidic liquid B is mixed with the alkaline liquid A, the mixed system is maintained at a specific pH value of 8.3-8.6, and the reinforcing agent is rapidly dissociated into phenoxy anions; and carrying out in-situ capture and enrichment on a micelle interface by CTAC cationic micelles with positive charges, which exist in the solution A in advance, through electrostatic attraction. And in cooperation with a low-ion-strength environment brought by no buffer salt in the liquid B, the system ensures the high activity of the HRP enzyme, realizes directional interface catalysis of the enhancer, and significantly improves the luminous efficiency and stability.

The invention relates to the technical field of immunosensors, in particular to a double-ligand Cu-MOF composite material, an immunosensor, a preparation method and application. The preparation method of the double-ligand Cu-MOF composite material comprises the following steps: dissolving isoluminol and 2-aminoterephthalic acid in a first organic solvent, then adding glutaraldehyde, and stirring under a dark condition to obtain a solution A; dissolving polyether F127 in a mixed solvent, and then adding copper salt to obtain a solution B; and mixing the solution A and the solution B, and carrying out a first heating reaction to obtain Cu-ABEI. The invention also provides the double-ligand Cu-MOF composite material prepared by adopting the method, an antigen-combined illuminant, an immunosensor and a preparation method of the double-ligand Cu-MOF composite material. According to the invention, the problem that the ECL signal stability of isoluminol is poor is solved, and the problem that the sensitivity is low when an existing sensor is used for detecting DBP is also solved.

The application relates to a luminol-embedded iron-based metal organic framework composite material and a preparation method and application thereof, and belongs to the technical field. The application discloses a preparation method of a luminol-embedded iron-based metal organic framework composite material (luminol@Fe-DOBDC MOFs), mainly adopting 2,5-dihydroxyterephthalic acid (DOBDC) as an organic linking agent, and Fe in FeCl3.6H2O 3+ As a metal node, Fe-DOBDC MOFs (Fe 3+ And the electronic interaction between the carboxyl groups, the fluorescence of the ligand is significantly quenched, and the stirring reaction with luminol can obtain a fluorescent double-emission composite material, that is, the luminol-embedded iron-based metal organic framework composite material (luminol@Fe-DOBDC MOFs).

The invention discloses an application of an improved isoluminol luminescent substrate in a MaglumiX8 chemiluminescence immunoassay system, and belongs to the technical field of in vitro diagnostic reagents. The luminescent substrate comprises an alkaline solution containing a metal leaf line complex and a derivative thereof, and a piperazine phenol derivative, and a peroxide solution containing urea and stannate. According to the invention, a metal leaf line substance is added into an alkaline solution, an active structure is stabilized through coordination modification, a reaction with hydrogen peroxide is promoted, a chemiluminescence signal is enhanced, and the luminous efficiency and the detection sensitivity are improved; by adding the piperazine phenol derivative, lasting glow of isoluminol is realized, signal stability is ensured, and result reliability is improved. In a hydrogen peroxide system, a urea / stannate stabilizer is added, so that the stability of a substrate is improved. Experimental verification shows that the substrate has high accuracy correlation with an original reagent, excellent precision and stable performance after being stored at room temperature for 24 months, and is suitable for the detection fields of hormones, tumor markers and the like.

This invention relates to the field of microfluidic sensing and detection technology, and more particularly to a photoelectric / fluorescence dual-mode self-calibrating microfluidic sensor for the detection of various mycotoxins, its fabrication method, and its application. The microfluidic sensor comprises a three-channel microfluidic chip and a substrate. In its fabrication, the three independent working electrodes and an integrated reference-counter electrode system are integrated into the microfluidic chip, utilizing oxygen-rich vacancy Io. 0.2~0.4 S 0.3~0.5 Using BiOBr (ISBO-OVs)@Au composite material as the sensing substrate, an internal secondary light source is formed by the chemiluminescence reaction of luminol-superoxide radicals, which can cascade and amplify the photocurrent signal. At the same time, combined with the specific recognition function of 7DCCA labeled aptamers, the sensitivity and specificity of microfluidic sensors for detecting trace fungal toxins are greatly improved.

A system and method for in-situ quantitative assessment of Bovine Mastitis (BM) in collected milk samples using optical imaging is disclosed. A bio-functionalized assay tray contains multiple wells, each having a gel with a specially prepared mixture of hemoglobin (Hb) and metal ions. At least one of the wells is filled with a reference milk sample which is characteristic of milk with no BM disease. Other wells are filled with collected milk samples. After waiting an interaction time, luminol and hydrogen peroxide solutions are added to the wells, causing chemiluminescent (CL) light emission. The intensity of CL light emitted by wells containing haptoglobin (Hp) is inhibited due to Hb-Hp binding. After waiting a CL light stabilization time, the tray is placed in a dark box, and a camera and image processor are used to analyze the CL light intensities. A calibration curve yields the Hp concentration in each well.

The present application belongs to the field of biosensors, and relates to a dual-mode sensor for detecting BACE1, in particular to a dual-mode sensor for detecting BACE1 and a preparation method and application thereof. The present application first uses silver nanoparticles co-modified by luminol and short peptides as an electrochemiluminescence and electrochemical dual-mode signal unit, uses a three-dimensional aggregate formed by CB[8] induced aggregation of silver nanoparticles co-modified by luminol and short peptides P2 as a signal probe, carries out signal labeling and signal amplification, and thus improves the sensitivity of BACE1 activity detection. It is worth noting that the dual-mode sensor provides diversified analysis data for the detection of BACE1 activity, and shows high selectivity and repeatability. Compared with a single-mode sensor, the dual-mode sensor can significantly improve the analysis efficiency and accuracy, and has important scientific significance and application value.

The invention belongs to the technical field of biomedical nano materials, and particularly relates to a phosphazene-based amphiphilic conjugate, an anti-inflammatory nano-micelle as well as a preparation method and application of the phosphazene-based amphiphilic conjugate and the anti-inflammatory nano-micelle. The structural formula of the phosphazene-based amphiphilic conjugate is shown in the specification. The anti-inflammatory nano-micelle based on the phosphazene-based amphiphilic conjugate can spontaneously form a micelle-shaped nano-structure with uniform particle size and high stability in an aqueous solution, and the solubility and the dispersity of insoluble active ingredients such as luminol and the like are remarkably improved; and the anti-inflammatory nano-micelle has the synergistic active oxygen scavenging ability of luminol and Tempol, can effectively regulate the oxidative stress microenvironment of an inflammatory site and relieve inflammatory response, and can be used as an inflammatory response carrier to realize targeted delivery of anti-inflammatory drugs.

This invention belongs to the field of electroluminescence technology, specifically relating to a method for preparing a high-entropy MOF material and its application in the electrochemiluminescence detection of rifampicin. The high-entropy MOF material is a pentagonal high-entropy MOF material (HE-ATA) synthesized from metal nitrates of Fe, Co, Cu, Cr, and Zn and ATA as raw materials. This invention yields a HE-ATA catalyst with simple raw materials, convenient synthesis, and excellent electrochemiluminescence performance. It exhibits chemical stability and a multi-electron reaction potential that accelerates the conversion of dissolved oxygen to reactive oxygen species (ROS), enhancing the electroluminescence intensity. Based on the quenching effect of RIF, a method for detecting RIF using a luminol / HE-ATA system was established. By fitting the change in ECL intensity with RIF concentration, a linear calibration curve with a correlation coefficient of 0.996 and a detection limit of 3.3 × 10⁻⁶ was obtained. ‑6 µM (S / N=3). This material exhibits good sensitivity, stability, and reproducibility in the electrochemiluminescence detection of RIFs, providing a new approach for designing novel electrocatalysts for RIF detection.

The application discloses a construction method and application of an electrochemiluminescence (ECL) antifouling sensor based on a zwitterionic hydrogel, and belongs to the fields of photoelectric sensing analysis, interface antifouling and environmental science and technology. In the application, a hydrogel based on a methacrylic acid sulfobetaine zwitterion and bovine serum albumin is used as an antifouling coating, so that the adsorption of biological proteins and bacteria is effectively hindered, and the service life of the sensor is prolonged. Meanwhile, an internal standard Au luminol is encapsulated in the antifouling hydrogel, so that the integration of interface antifouling and ratio detection is realized, the detection accuracy is improved, and the construction process of the ECL sensor is simplified. The constructed ECL antifouling sensor has high sensitivity for chloramphenicol detection, and the detection limit is 0.39 pM, so that the ECL antifouling sensor is suitable for trace detection of antibiotics in seawater.

The invention belongs to the technical field of electrochemical catalysis and detection, and particularly relates to application of a quinary high-entropy MOF material modified electrode in electrochemical catalytic oxygen reduction reaction (ORR) and reactive oxygen species (ROS) detection. The method comprises the following steps: weighing 5 mmol of nitrate of Fe, Co, Cr, Cu and Zn, dissolving the nitrate in a mixed solution containing 70 mL of DMF, 6 mL of ethanol and 6 mL of water, and stirring at 25 DEG C at a rotating speed of 2500 r / min for 8 hours; and adding 0.22 mol of terephthalic acid (TPA) for dissolving, putting the solution into a high-pressure kettle, reacting for 24 hours under the airtight condition of 150 DEG C, collecting the product, centrifuging, washing and drying to obtain the five-membered high-entropy compound FeCoCuCrZn-MOF [HEMOF (BDC)]. According to the present invention, the catalyst with characteristics of easily available raw materials, simple process and excellent electrochemical performance is obtained, the ORR catalysis efficiency is high, the stability is good, and the ROS detection method based on the luminol ECL system is established. The material has good sensitivity, stability and reproducibility when being used for electrochemiluminescence detection of ROS, and a new scheme for detecting ROS through an electrocatalyst is provided.

The present application relates to the technical field of in vitro diagnosis, in particular to a chemiluminescence-based adiponectin detection method, which uses MQ silicon resin-coated Fe3O4 magnetic microspheres as a solid-phase carrier, captures adiponectin in a sample through a double-antibody sandwich immunoreaction, and uses HRP enzyme-labeled detection antibodies to combine with a luminol-phenolic enhanced chemiluminescence system for signal detection. The key lies in significantly reducing non-specific background by optimizing washing conditions and substrate formula, and regulating luminescence kinetics to adapt to the reading window of mainstream chemiluminescence instruments. The present application uses HRP enzyme-catalyzed cascade amplification and an optimized luminol enhancement system, and the detection lower limit is better than that of conventional methods, meeting the needs of early metabolic risk screening for ultra-low concentration detection, the linear detection range covers 0.01 mg / L to 100 mg / L, effectively avoiding the common hook effect in high-concentration samples, and the signal-to-noise ratio is greatly improved, ensuring the reliability of low-value sample detection.

The present disclosure provides for imaging agents and methods of making and using the same. The imaging agent comprises a luminol component comprising a luminol moiety or functional analogue thereof; a chelator; a linker group bonding or complexing the luminol component to the chelator; and a radiolabel component bound to the chelator. The imaging agent described herein is capable of monitoring, targeting, or imaging oxidative stress, reactive oxygen species (ROS), superoxide generation, or hydrogen peroxide generation, mediating pathophysiology of different disease states.

This invention relates to the field of biosensor technology, and in particular to an electrochemiluminescence sensor, its fabrication method, and its applications. The sensor comprises a three-electrode system, wherein the working electrode includes a substrate electrode, an amino-functionalized vertically ordered mesoporous silica film formed on the surface of the substrate electrode, and nitrogen-doped graphene quantum dots confined within the amino-functionalized vertically ordered mesoporous silica film; the electrolyte used in the sensor is a phosphate buffer containing luminol. This invention achieves highly sensitive and selective detection of superoxide dismutase (SOD) by constructing an electrode modified with nitrogen-doped graphene quantum dots confined within an amino-functionalized vertically ordered mesoporous silica film, utilizing its enhanced luminol-dissolved oxygen electrochemiluminescence signal and the specific quenching effect of SOD on superoxide anions.

The invention provides an Ag (at) SiO2 (at) mSiO2 (at) luminol composite material, a preparation method, an unmarked electrochemical luminescence sensor and application, and relates to the technical field of metal ion detection. The Ag (at) SiO2 (at) mSiO2 (at) luminol nano composite material has a core-shell structure, the core-shell structure sequentially comprises silver nanoparticles, a silicon dioxide layer, a mesoporous silicon dioxide layer and luminol molecules from inside to outside, and the luminol molecules are embedded into mesopores of the mesoporous silicon dioxide layer. The label-free electrochemical luminescence sensor constructed based on the material realizes rapid, low-cost and ultra-sensitive detection of Hg < 2 + >.

The invention discloses a method for improving the intensity of an N-(4-aminobutyl)-N-ethyl isoluminol cathode electrogenerated chemiluminescence signal and application of the method. A novel green and efficient signal enhancement method is established, and the problem of biotoxicity of a traditional co-reactant is solved. According to the method, an innovative solution is provided for N-(4-aminobutyl)-N-ethyl isoluminol cathode electrogenerated chemiluminescence signal enhancement, and a new technical path is provided for application expansion of the field of trace analysis and detection.

The invention discloses an anti-pollution and sterilization integrated non-co-reactant electrochemical luminescence (ECL) sensor for detecting staphylococcus aureus in an aquatic product as well as a preparation method and an application method of the anti-pollution and sterilization integrated non-co-reactant ECL sensor, and the anti-pollution and sterilization integrated non-co-reactant ECL sensor is suitable for ultra-sensitive detection of the staphylococcus aureus. The integrated hydrogel illuminant is characterized in that chitosan and citral are subjected to a Schiff base reaction to form a three-dimensional network in a cross-linking manner, and tetrakis (4-hydroxyphenyl) porphyrin (THPP) and luminol are introduced into the three-dimensional network. According to the present invention, THPP is adopted as a high efficiency oxygen evolution reaction (OER) electrocatalyst to generate reactive oxygen species (ROS) to replace the traditional hydrogen peroxide so as to be adopted as a co-reactant, such that the ECL signal of luminol is significantly enhanced; the hydrogel has excellent hydrophilicity and antibacterial activity, can significantly reduce non-specific adsorption, realizes anti-pollution detection, and realizes sterilization treatment after detection. By combining a DNA Walker signal amplification strategy, the sensor has a linear response to S. aureus within the range of 101-108 CFU / mL, the detection limit is as low as 0.39 CFU / mL, and the recovery rate of 97.3%-111.85% is obtained in a marine product actual sample. The device has the advantages of being simple in structure, sensitive in detection, high in anti-interference capacity, capable of achieving on-site rapid detection and the like, and is suitable for the fields of food safety, environment monitoring and clinical pathogenic bacterium detection.

The invention discloses a plug-and-play ultra-sensitive clustered peptidyl material and a preparation method and application of a visual ratio nano fluorescent probe of the plug-and-play ultra-sensitive clustered peptidyl material, and belongs to the technical field of nano composite material preparation. The invention solves the problems of complexity, low speed, low sensitivity and the like of the current bacillus anthracis marker and tetracycline detection method. A clustered peptidyl material PG-COOH is constructed, the PG-COOH and lanthanide ions are co-assembled to obtain a probe Eu / Tb-PG-COOH for detecting the DPA, meanwhile, a probe PNPG-Eu-LML for detecting the tetracycline in which the PG-COOH and luminol cooperatively bridge Eu < 3 + > is constructed, and the two probes both take clustered peptide-Eu < 3 + > as cores and have coordination stability; plug-and-play coupling of the identification unit and the fluorescence signal unit is realized, background interference is reduced in cooperation with a ratio fluorescence double-signal calibration mechanism, and visual output supports on-site rapid detection.

The application relates to preparation and application of a sandwich type ECL immunosensor of a H2O2 / O2 system for signal amplification by using luminol as a luminophore and a double metal oxide, and belongs to the field of biosensing and novelty combination of new functional materials. A nickel-manganese double metal oxide (NiMnO3@Au-luminol) loaded with luminol luminophore is combined with a gold-modified cobalt-cerium double metal oxide (CoCeOx@Au) to serve as an electrochemiluminescence sensing platform, a sandwich type electrochemiluminescence immunosensor is constructed, and the sensor is used for super-sensitive detection of a non-small cell lung cancer marker CYFRA21-1. The NiMnO3 and CoCeOx materials have great application potential due to simple preparation, suitable acid-base property and multiple valence states of metals. The NiMnO3 can catalyze O2 to generate O2 •− , the CoCeOx can promote H2O2 to decompose to generate •OH, both of the two free radicals can act on Luminol •− to generate a stronger luminescence signal; Au modification on the material surface can significantly improve the electron transfer rate, increase the number of antigen-antibody combination, enhance the ECL signal, and realize sensitive and accurate detection of biomolecules.

The application discloses a kind of photochemical reaction long afterglow luminescence photosensitizer materials, nanoparticles and preparation method and application thereof.The photochemical reaction long afterglow luminescence photosensitizer materials are obtained by reacting 3,4-disubstituted maleic anhydride derivatives with isoluminol or isoluminol derivatives containing amino group;The substituent group of the 3,4-disubstituted maleic anhydride derivative is R1 and R2.The photochemical reaction long afterglow luminescence photosensitizer materials of the application realize high signal-to-noise ratio in vivo by photochemical reaction long afterglow luminescence, long-life fluorescent imaging, and have important application prospect in efficient clinical PDT treatment.

This invention discloses a substrate solution formulation and preparation method for the Antu A2000 fully automated chemiluminescence immunoassay analyzer, belonging to the field of in vitro diagnostic reagent technology. The substrate solution includes solution A and solution B, wherein solution A consists of hydrogen peroxide, a buffer, and a hydrogen peroxide stabilizer; solution B consists of purified luminol, a luminescence enhancer, and a buffer. The hydrogen peroxide stabilizer is selected from HEDP, 8-hydroxyquinoline, urea, or sodium polyacrylate; the luminescence enhancer is selected from 4-morpholinopyridine, p-iodophenol, or imidazole; luminol is purified by ethanol-water gradient recrystallization and ether extraction. The preparation method includes: weighing and dissolving the components, adjusting the pH, stirring and filtering, and dispensing into opaque containers. This invention uses a stabilizer to improve the stability of hydrogen peroxide, an enhancer to increase the luminescence intensity, and reduces impurity interference, resulting in high sensitivity and low cost. It can completely replace the original reagents, exhibiting significant economic and social benefits.