Preparation of chemiluminiscence light-induced electrochemical sensor and organic phosphorus pesticide detection

Abstract

The invention relates to preparation of a chemiluminiscence light-induced electrochemical sensor and organic phosphorus pesticide detection. Preparation is achieved through the steps that gold nanoparticles are electrochemically deposited on conductive glass; zinc oxide nanorods are prepared on the gold nanoparticles deposited on the conductive glass through a seed growing mode; CdS electrochemical deposition is conducted in an electro-deposition solution prepared from cadmium nitrate, thiourea, dimethylsulfoxide and water to obtain CdS quantum dots loaded on the ZnO nanorod surfaces; a molecularly-imprinted polymer film loaded on the surface of an electrode is prepared by taking an organic phosphorus pesticide as a template molecule, taking methacrylic acid as a functional monomer, taking dimethylacrylicacid glycol ester as a cross-linking agent, taking acetonitrile as a pore-forming agent and taking azodiisobutyronitrile as an initiating agent; a chemiluminescent reagent N-(4-ammonia butyl)-N-ethylisoluminol is covalently connected to the molecularly-imprinted film; the template molecule in the molecularly-imprinted polymer is eluted with methanol and glacial acetic acid, and then the chemiluminiscence light-induced electrochemical sensor is prepared. Chemiluminiscence generated by hydrogen peroxide and N-(4-ammonia butyl)-N-ethylisoluminol is taken as a light source, ZnO and CdS are excited by light to generate light currents, and the sensor can be applied to organic phosphorus pesticide detection.

Description

technical field [0001] The invention relates to the technical field of pesticide residue detection, in particular to the preparation of a chemiluminescent photoelectrochemical sensor and the detection of organophosphorus pesticides. Background technique [0002] my country is a big agricultural country, and it is also a big country in the production and use of pesticides. In addition, the structure of pesticides is still unreasonable, the monitoring system of pesticide residues is not perfect, and users have insufficient awareness of the hazards of pesticides, which makes the problem of pesticide residues particularly prominent in my country. According to relevant statistics, there are three 70% in the use of pesticides in my country, namely: pesticides account for 70% of pesticides; organophosphorus species account for 70% of insecticides; Aminophos, monocrotophos, parathion, methyl parathion, omethoate, dichlorvos, etc. account for 70% in my country. Pesticide residues wi...

AI Technical Summary

Problems solved by technology

However, the types of pesticides and samples detected by these methods are often limited, all toxic substances in the samples may respond, the types of residues cannot be identified, the accuracy is low, and the preparation of antibodies in the early stage is difficult, and the development cost is high. It takes a long time, and the high specificity of the antibody makes it only suitable for the detection and analysis of one or a type of pesticide residues, which limits the scope of application of this method