10 results about "Methyl parathion" patented technology

The present invention discloses a preparation method of an enhanced hydrolytic nanozyme for the degradation of organophosphorus pesticides. The broad-spectrum nucleic acid aptamer BSAPT is modified on the surface of a metal zirconium organic framework MOF-808 in various ways to form a MOF-808 / BSAPT or MOF-808-BSAPT enhanced hydrolytic nanozyme for selective and efficient degradation of organophosphorus pesticides. The enhanced hydrolytic nanozyme prepared by the present invention can simulate phosphatase activity and broadly identify a variety of highly toxic organophosphorus pesticides. The organophosphorus pesticide broad-spectrum nucleic acid aptamer BSAPT actively recognizes and captures organophosphorus compounds, enriches the target molecule to the Lewis acidic Zr(IV) active site center of MOF-808, and achieves selective and efficient degradation of organophosphorus pesticides by nucleophilic attack on the electrophilic group of the phosphorus atom center of the organophosphorus compound substrate. It can effectively degrade a variety of highly toxic organophosphorus pesticides such as chlorpyrifos, profenofos, and methyl parathion. The invention has simple preparation materials, low cost, fast degradation rate and broad spectrum, and can be applied to the degradation of various organophosphorus pesticides in water bodies.

The present invention discloses a pinanyl fluorescent probe for detecting the organophosphorus pesticide methyl parathion based on an enzyme inhibition mechanism, as well as its preparation method and application. The fluorescent probe is: 4-(2-(4-(cyclopropylformyloxy-4′-(6,6-dimethyl-2-(4-nitrophenyl)-4,5,6,7-tetrahydro-2H-5,7-methyleneindazole-3-yl)-[1,1′-biphenyl]-3-yl)vinyl)-1-methylpyridinium iodide (abbreviated as: THIP-OCP). The compound can undergo enzymatic hydrolysis reaction with butyrylcholinesterase and, under irradiation with ultraviolet light at a wavelength of 365nm, The solution's fluorescence color changes from colorless to orange-yellow. Since methyl parathion effectively inhibits butyrylcholinesterase activity, when methyl parathion is added to the solution, the solution's fluorescence gradually changes from orange-yellow to colorless under ultraviolet irradiation at a wavelength of 365 nm. Therefore, this compound can be used as a fluorescent probe for detecting methyl parathion. This compound has many advantages, including a low detection limit (0.79 μg / mL) and a wide pH range (5-10), and has great application prospects.

The application provides a kind of gold nanoclusters with polypeptide as ligand and detection method of methyl parathion, the sequence of polypeptide in the gold nanoclusters with polypeptide as ligand is H (S, D) GGFFYCC;The gold nanoclusters with polypeptide as ligand can be used for double signal detection of methyl parathion and detection of methyl parathion in fruits and vegetables.Fluorescence signal detection: gold nanoclusters are added to the test solution, 37 DEG C reaction 30-60 min, detect the fluorescence change at 700 nm.Colorimetric signal: in pH=8.0 buffer solution, different concentrations of methyl parathion, acetylcholinesterase, choline oxidase and acetylcholine are added, 37 DEG C preheating 30-60 min.pH=4.0 buffer solution, gold nanoclusters and TMB are added, 37 DEG C reaction 10 min, 652 nm is measured absorbance.The detection method of the application has high detection sensitivity, strong anti-interference ability, low detection limit, high correlation coefficient of detection curve, good reproducibility in fruit and vegetable detection recovery experiment, and has good practical value in the detection of methyl parathion.

The present invention provides a methyl parathion hapten, a sibutramine hapten, a methyl parathion-sibutramine artificial antigen and antibody, and a detection kit, relating to the technical field of food safety detection. The polyclonal antibody obtained by immunization with the artificial antigen not only has high sensitivity but also can simultaneously identify both methyl parathion and sibutramine, thus solving the technical problem of the lack of a highly sensitive and rapid method for the simultaneous detection of methyl parathion and sibutramine in the prior art. The half inhibitory concentration (IC50) of the antibody for methyl parathion is 100%. 50 ) was 4.59 ng / mL, the quantitative detection range was 2.89 ng / mL-7.28 ng / mL, and the detection limit was 2.21 ng / mL; the half-inhibitory concentration (IC 50 ) was 59.76 ng / mL, the quantitative detection range was 37.65 ng / mL-94.87 ng / mL, and the detection limit was 28.73 ng / mL.

This invention belongs to the field of pesticide residue detection technology, specifically relating to a method for preparing a cerium-based covalent organic framework composite material with alkaline phosphatase activity and its application. Using COF, L-valine, and Ce(NO3)3·6H2O as raw materials, a COF-OMe@Valine-CeO2 nanozyme was prepared via in-situ growth. This nanozyme exhibits alkaline phosphatase activity, and the constructed sensor demonstrates good selectivity, repeatability, and stability. The preparation method of the CeO2-Val@COF-OMe nanozyme is simple and can achieve rapid detection and hydrolysis of the organophosphorus pesticide methyl parathion.

This invention discloses a ratiometric electrochemical sensor, its preparation method, and a method for simultaneously detecting methyl parathion and carbendazim. The sensor uses a CeCoZn-MOF / NBA / CNHs-modified glassy carbon electrode as the working electrode, a saturated KCl-type Ag / AgCl as the reference electrode, and a platinum wire as the counter electrode. Preparation involves electrode pretreatment, CeCoZn-MOF synthesis, composite material compounding, dispersion preparation, and electrode drop-coating modification. Differential pulse voltammetry is used for detection, with Nile Blue A as the internal standard probe. Quantification is based on the current ratio of the target analyte to the internal standard. The linear detection range for methyl parathion is 0.05–15 μg / mL, and for carbendazim, it is 0.05–4 μg / mL, with correlation coefficients greater than 0.99 for both. This sensor exhibits strong anti-interference capabilities, high sensitivity and accuracy, with a recovery rate of 91.0%–109.2% in actual agricultural product samples. It enables rapid, simultaneous, and accurate detection of methyl parathion and carbendazim, and is suitable for rapid on-site detection of pesticide residues in agricultural products.

The application discloses a preparation method of a cross-linked enzyme aggregate biological catalytic membrane, and mainly comprises the following steps: taking a polyacrylonitrile ultrafiltration membrane as a base film, loading polydopamine modified UiO-66-NH2 nanoparticles to the surface of the polyacrylonitrile ultrafiltration membrane as an intermediate layer, the adsorption characteristics of the intermediate layer help to enrich micro-pollutants and promote enzyme catalysis, and loading cross-linked enzyme aggregates on the treated polyacrylonitrile ultrafiltration membrane by a pressure-assisted self-assembly technology, so as to obtain the cross-linked enzyme aggregate biological catalytic membrane. The preparation method is simple in operation and easy to implement. The cross-linked enzyme aggregate technology is used to combine separation, purification and immobilization together, one-step purification and immobilization of the enzyme are realized, and the immobilization amount is increased. The cross-linked enzyme aggregate biological catalytic membrane is used for removing micro-pollutants in water, separating dyes and salt ions in wastewater or removing methyl parathion in water, and the removal rate is more than 90%, so that the cross-linked enzyme aggregate biological catalytic membrane has good effects.

The present invention relates to the technical field of methyl parathion detection, and in particular to an AChE / AgNPs@PVA / RGO / SPE sensor and its preparation and application. Based on the inhibitory effect of methyl parathion on acetylcholinesterase, the present invention prepares a sensor for methyl parathion detection. The sensor has good electrocatalytic ability for the enzymatic oxidation of thiocholine, and exhibits excellent adsorption capacity and high SERS activity for methyl parathion; the inhibitory effect of methyl parathion on AChE is proportional to its concentration. The sensor is used for electrochemical detection and Raman spectroscopy detection of methyl parathion, and a linear relationship between the methyl parathion concentration and the current signal intensity and the Raman signal intensity is obtained. Compared with existing on-site rapid detection methods, the present invention has good stability, more SERS active hotspots, high sensitivity and is easy to carry, providing a new method for the detection of organophosphorus pesticides.

The application discloses a GR@TA-Ag@MIH electrode modification material and application thereof, tannic acid (TA) is dissolved in deionized water, a Tris solution is added to adjust the pH to 7.5-8.5, and then AgNO3 is added to obtain a TA-Ag solution after stirring; graphene (GR) is dissolved in the TA-Ag solution to form a GR@TA-Ag suspension; the GR@TA-Ag suspension is added into a mixed solution containing acrylamide (AM), ammonium persulfate (APS) and methyl parathion (MP), and is stirred for 5-15 min, and after reaction, washing is performed to obtain the GR@TA-Ag@MIH electrode modification material. The graphene in the GR@TA-Ag@MIH composite material endows the sensor with excellent conductive performance, and the introduced metal ions further improve the conductive characteristics of the composite material, the sensor realizes high specificity identification of methyl parathion, and effectively avoids the interference of compounds similar in structure from generating an oxidation peak at the same potential; a TA-Ag double-catalytic system is constructed, free radicals are rapidly polymerized at room temperature, the self-adhesion of the hydrogel is utilized, and the binding force of the GR@TA-Ag@MIH and the electrode and the adsorption capacity of methyl parathion are improved.

This invention provides a biocatalytic composition for degrading soil organophosphorus residues and its preparation method, belonging to the field of soil remediation technology. The biocatalytic composition provided by this invention comprises: 20-35 parts of a compound microbial agent, 15-25 parts of an organophosphorus hydrolase, 30-45 parts of a soil amendment component, 5-10 parts of a protectant, and 2-6 parts of a synergist; the compound microbial agent is composed of *Pseudomonas aeruginosa*, *Rhizopus oligosporus*, and *Bacillus subtilis*, and the organophosphorus hydrolase is a modified immobilized organophosphorus hydrolase. In the biocatalytic composition of this invention, the compound microbial agent and the modified immobilized enzyme work synergistically to rapidly and efficiently degrade various organophosphorus residues in the soil, such as chlorpyrifos, methyl parathion, and triazophos, achieving a synergistic improvement in soil fertility and remediation effect. It is suitable for in-situ remediation of soil contaminated by organophosphorus pesticides, such as farmland, orchards, and greenhouses.