Preparation of carbaryl and methyl parathion universal antibody and universal envelope antigen

A methyl parathion, coating antigen technology, applied in chemical instruments and methods, animal/human proteins, serum albumin, etc., can solve the problem of not simultaneous analysis of enzyme-linked immunosorbent assay kits and their preparation methods, etc. , to achieve the effect of simple pretreatment process and improved sensitivity

Inactive Publication Date: 2009-07-08
孙家隆
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Problems solved by technology

Current documents and patents disclose an enzyme-linked immunosorbent assay kit for analyzing residual carbaryl or an enzyme-linked immunosorbent assay kit for analyzing residual methyl parathi

Method used

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  • Preparation of carbaryl and methyl parathion universal antibody and universal envelope antigen
  • Preparation of carbaryl and methyl parathion universal antibody and universal envelope antigen
  • Preparation of carbaryl and methyl parathion universal antibody and universal envelope antigen

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Embodiment Construction

[0034] The present invention will be further described below with embodiment:

[0035] Preparation of artificial antigens: 60 mg and 72 mg of artificial haptens of carbaryl and methyl parathion were dissolved in 5.0 ml of DMF. Dissolve 0.2 mL of tri-n-butylamine in 5.0 mL of DMF, and add e to d to obtain f. Dissolve 0.16 mL of isobutyl chloroformate in 10 mL of DMF, add dropwise to f at 4°C, and react for 1 hour to obtain g. 100 mg of bovine serum albumin was dissolved in 60 mL of borate buffer solution with pH=8.7 and 0.05 M, then g was added dropwise to it below 18°C, and reacted at room temperature (~20°C) for 12 hours. The obtained reaction solution was put into a dialysis bag, and dialyzed in PH=7.4, 0.01M buffer solution at 4°C for 60 hours, and the PBS dialysate was changed every 8 hours, 1 L each time. Until no hapten UV absorbance (OD 282 ). Accurately measure the volume of the protein solution after dialysis, determine the protein concentration and binding ratio ...

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Abstract

The invention discloses the preparation for a carbaryl and methyl parathion general antibody and general envelope antigen suitable for an enzyme-linked immunosorbent assay kit capable of simultaneously carrying out the carbaryl and methyl parathion retention analysis. The invention comprises a preparation method for a carbaryl artificial hapten and methyl parathion artificial hapten structure, and the carbaryl and methyl parathion general antibody and general envelope antigen. The carbaryl and methyl parathion general antibody and general envelope antigen can be used for the preparation of the enzyme-linked immunosorbent assay kit capable of simultaneously carrying out the carbaryl and methyl parathion retention analysis. The kit can be used for the quick detection of the carbaryl residue and methyl parathion in the water, soil, vegetable, food and poisoned samples. The preliminary treatment of the samples is simple; the kit can be used for the detecting a batch quantity of samples; and the sample detection cost is lower than that of a conventional detection method.

Description

(1) Technical field [0001] The invention belongs to the field of residual pesticide detection, and relates to an enzyme-linked immunosorbent assay kit suitable for analyzing residual carbaryl and methyl parathion at the same time, and a universal antibody for carbaryl and methyl parathion and a universal package Antigen preparation. (2) Background technology [0002] The traditional residue analysis methods of pesticides and their metabolites mainly rely on physical and chemical analysis methods such as gas chromatography (GC), high performance liquid chromatography (HPLC), and mass spectrometry (MS). The chronic and long-term effects of human health and human health are increasingly concerned and worried, and the restrictions on pesticide residues are becoming more and more stringent. New requirements and higher requirements are put forward for the analysis and measurement objects, types, quantities, ranges, indicators, etc. However, the traditional physical and chemical a...

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Application Information

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IPC IPC(8): C07K14/765C07K16/44C07K16/06G01N33/543
Inventor 孙家隆
Owner 孙家隆
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