Preparation of carbaryl and methyl parathion universal antibody and universal envelope antigen
A methyl parathion, coating antigen technology, applied in chemical instruments and methods, animal/human proteins, serum albumin, etc., can solve the problem of not simultaneous analysis of enzyme-linked immunosorbent assay kits and their preparation methods, etc. , to achieve the effect of simple pretreatment process and improved sensitivity
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[0034] The present invention will be further described below with embodiment:
[0035] Preparation of artificial antigens: 60 mg and 72 mg of artificial haptens of carbaryl and methyl parathion were dissolved in 5.0 ml of DMF. Dissolve 0.2 mL of tri-n-butylamine in 5.0 mL of DMF, and add e to d to obtain f. Dissolve 0.16 mL of isobutyl chloroformate in 10 mL of DMF, add dropwise to f at 4°C, and react for 1 hour to obtain g. 100 mg of bovine serum albumin was dissolved in 60 mL of borate buffer solution with pH=8.7 and 0.05 M, then g was added dropwise to it below 18°C, and reacted at room temperature (~20°C) for 12 hours. The obtained reaction solution was put into a dialysis bag, and dialyzed in PH=7.4, 0.01M buffer solution at 4°C for 60 hours, and the PBS dialysate was changed every 8 hours, 1 L each time. Until no hapten UV absorbance (OD 282 ). Accurately measure the volume of the protein solution after dialysis, determine the protein concentration and binding ratio ...
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