7 results about "Parathion" patented technology

The plant source insecticide contains effective components of parathion and avermectin, the weight ratio of parathion and avermectin is 10-50:1, and is supplemented with an acceptable agricultural adjuvant to form one of emulsifiable concentrate, wettable powder, water dispersible granule, water emulsion or suspension agent suitable for agricultural use, and belongs to the field of pesticides. The plant source insecticide shows activity on peridroma saucia, aphid and cotton red spider, especially high acaricidal activity on red spider. The prevention and treatment effect on citrus red spider is obviously improved after parathion and avermectin are compounded, which shows that the combination of parathion and avermectin has significant synergistic effect on citrus red spider. Parathion is derived from Chinese herbal medicine plant, and there is no problem of pesticide residue, and the risk of drug damage is greatly reduced. At the same time, the two single agents of the composition have great difference in chemical structure and completely different action mechanism, and there is no cross resistance, which can delay the resistance problem caused by the use of the two single agents alone.

The invention discloses a high-sensitivity wide-spectrum specific antibody for resisting parathion, parathion-methyl and fenitrothion, the high-sensitivity wide-spectrum specific antibody comprises a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is as shown in SEQ ID NO: 2; the amino acid sequence of the light chain variable region coding gene is shown as SEQ ID NO: 4. The invention also provides an antibody expression plasmid. The obtained variable region sequence gene is respectively connected to an expression vector containing a heavy chain constant region gene and a light chain constant region gene, and a new recombinant full-length antibody is successfully obtained through an HEK293 (F) mammalian cell expression system. Based on the characteristics of stability and easy preservation of recombinant expression plasmids, the recombinant expression plasmids can be used for multi-batch repeated production of prohibited and limited organophosphorus pesticide recombinant antibodies for resisting parathion and the like; a stable and reliable core reaction reagent is provided for construction of an immunoassay method for multiple residues of organophosphorus pesticides such as parathion in a development environment and a food sample and development of a field screening product.

The invention discloses a UiO-66-NH2 (at) MWCNTs (at) CeO2 composite material, a preparation method thereof and a method for preparing an electrochemical sensor, and particularly relates to the technical field of pesticide residue detection. The core UiO-66-NH2 (at) MWCNTs (at) CeO2 composite material provided by the invention is prepared from the following components: UiO-66-NH2, MWCNTs and CeO2, and the mass ratio of the UiO-66-NH2 to the MWCNTs to the CeO2 is (2 to 10): 1: (1 to 5). The invention provides a UiO-66-NH2 (at) MWCNTs (at) CeO2 composite material, a preparation method of the UiO-66-NH2 (at) MWCNTs (at) CeO2 composite material and a method for preparing an electrochemical sensor. According to the research, through experimental optimization, an electrochemical sensing platform based on the UiO-66-NH2 (at) MWCNTs (at) CeO2 ternary composite material is successfully constructed, and high-performance synchronous detection of typical pollutants such as cadmium, parathion-methyl and mercury in a sample to be detected is realized.

The present application relates to the technical field of microbial pesticide pollution remediation, and specifically discloses a strain capable of efficiently degrading MCPA and application thereof. The strain is Priestia megaterium AS, and the preservation number is CCTCC No. M 20242160. The present application provides a method for isolating and screening the strain, which grows well in a culture medium containing 500 μmol / L MCPA and can degrade MCPA as a carbon source, with a degradation rate of 500 μmol / L MCPA exceeding 50% within 7 days. It is proved by a potting test that inoculation of the strain or its preparation can significantly reduce the MCPA residue in the rhizosphere soil, effectively alleviate the toxic effect of MCPA on sensitive crops such as melons and pumpkins, and promote the growth recovery of plants. The strain and its bacterial agent provide an efficient and environmentally friendly biological technology solution for remediation of MCPA contaminated soil and guarantee of safe production of melon crops.

This invention belongs to the field of food and agricultural product safety testing technology, specifically relating to a rapid detection method for parathion and its application. Based on the parathion-induced blue shift in fluorescence of silicon nanoparticles, ascorbic acid is reacted with 3-aminopropyltriethoxysilane to generate green fluorescent silicon nanoparticles. During the formation of silicon nanoparticles, parathion can embed itself into the porous structure of the silicon nanoparticles through host-guest interactions, causing a change in the excited state of the silicon nanoparticles. This affects the fluorescence emission of the silicon nanoparticles, resulting in a blue shift in the fluorescence emission peak, changing from green fluorescence to blue fluorescence, specifically manifested as an increase in fluorescence intensity at 422 nm and a decrease in fluorescence intensity at 520 nm. By establishing the relationship between ratiometric fluorescence signal or fluorescence color change and parathion concentration, specific detection of parathion is achieved. This invention requires no enzymes or antibodies for parathion detection, is inexpensive, fast, easy to operate, highly sensitive, and has good specificity.

This invention discloses a ratiometric electrochemical sensor, its preparation method, and a method for simultaneously detecting methyl parathion and carbendazim. The sensor uses a CeCoZn-MOF / NBA / CNHs-modified glassy carbon electrode as the working electrode, a saturated KCl-type Ag / AgCl as the reference electrode, and a platinum wire as the counter electrode. Preparation involves electrode pretreatment, CeCoZn-MOF synthesis, composite material compounding, dispersion preparation, and electrode drop-coating modification. Differential pulse voltammetry is used for detection, with Nile Blue A as the internal standard probe. Quantification is based on the current ratio of the target analyte to the internal standard. The linear detection range for methyl parathion is 0.05–15 μg / mL, and for carbendazim, it is 0.05–4 μg / mL, with correlation coefficients greater than 0.99 for both. This sensor exhibits strong anti-interference capabilities, high sensitivity and accuracy, with a recovery rate of 91.0%–109.2% in actual agricultural product samples. It enables rapid, simultaneous, and accurate detection of methyl parathion and carbendazim, and is suitable for rapid on-site detection of pesticide residues in agricultural products.

The present application belongs to the technical field of pesticide detection, and particularly relates to a multi-in-one pesticide residue quantitative detection test paper and a detection method. The test paper is provided with gold nanoparticle labeled antibodies of at least two kinds of pesticides on a colloidal gold combination pad; a chromatography membrane is provided with a quality control line and at least two detection lines, each detection line is coated with an antigen of one kind of pesticide; the pesticides are selected from chlorpyrifos, phorate, dimethoate, acetamiprid, carbendazim, procymidone, fenpropathrin, carbofuran, isoprocarb, chlorothalonil, chlorfenapyr, parathion, tetramethylenedisulfide, triadimefon, isocarbophos or parathion. Through optimization of the pesticide types and preparation methods of the antigens and antibodies, the present application overcomes the problem of mutual interference of detection targets when multiple pesticides are simultaneously detected, has good specificity, high sensitivity, can accurately quantify, can simultaneously detect multiple components, and has simple pretreatment, and thus has good application prospect.