15 results about "Parathion" patented technology

The present invention discloses a preparation method of an enhanced hydrolytic nanozyme for the degradation of organophosphorus pesticides. The broad-spectrum nucleic acid aptamer BSAPT is modified on the surface of a metal zirconium organic framework MOF-808 in various ways to form a MOF-808 / BSAPT or MOF-808-BSAPT enhanced hydrolytic nanozyme for selective and efficient degradation of organophosphorus pesticides. The enhanced hydrolytic nanozyme prepared by the present invention can simulate phosphatase activity and broadly identify a variety of highly toxic organophosphorus pesticides. The organophosphorus pesticide broad-spectrum nucleic acid aptamer BSAPT actively recognizes and captures organophosphorus compounds, enriches the target molecule to the Lewis acidic Zr(IV) active site center of MOF-808, and achieves selective and efficient degradation of organophosphorus pesticides by nucleophilic attack on the electrophilic group of the phosphorus atom center of the organophosphorus compound substrate. It can effectively degrade a variety of highly toxic organophosphorus pesticides such as chlorpyrifos, profenofos, and methyl parathion. The invention has simple preparation materials, low cost, fast degradation rate and broad spectrum, and can be applied to the degradation of various organophosphorus pesticides in water bodies.

The plant source insecticide contains effective components of parathion and avermectin, the weight ratio of parathion and avermectin is 10-50:1, and is supplemented with an acceptable agricultural adjuvant to form one of emulsifiable concentrate, wettable powder, water dispersible granule, water emulsion or suspension agent suitable for agricultural use, and belongs to the field of pesticides. The plant source insecticide shows activity on peridroma saucia, aphid and cotton red spider, especially high acaricidal activity on red spider. The prevention and treatment effect on citrus red spider is obviously improved after parathion and avermectin are compounded, which shows that the combination of parathion and avermectin has significant synergistic effect on citrus red spider. Parathion is derived from Chinese herbal medicine plant, and there is no problem of pesticide residue, and the risk of drug damage is greatly reduced. At the same time, the two single agents of the composition have great difference in chemical structure and completely different action mechanism, and there is no cross resistance, which can delay the resistance problem caused by the use of the two single agents alone.

The invention discloses a high-sensitivity wide-spectrum specific antibody for resisting parathion, parathion-methyl and fenitrothion, the high-sensitivity wide-spectrum specific antibody comprises a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is as shown in SEQ ID NO: 2; the amino acid sequence of the light chain variable region coding gene is shown as SEQ ID NO: 4. The invention also provides an antibody expression plasmid. The obtained variable region sequence gene is respectively connected to an expression vector containing a heavy chain constant region gene and a light chain constant region gene, and a new recombinant full-length antibody is successfully obtained through an HEK293 (F) mammalian cell expression system. Based on the characteristics of stability and easy preservation of recombinant expression plasmids, the recombinant expression plasmids can be used for multi-batch repeated production of prohibited and limited organophosphorus pesticide recombinant antibodies for resisting parathion and the like; a stable and reliable core reaction reagent is provided for construction of an immunoassay method for multiple residues of organophosphorus pesticides such as parathion in a development environment and a food sample and development of a field screening product.

The present invention discloses a pinanyl fluorescent probe for detecting the organophosphorus pesticide methyl parathion based on an enzyme inhibition mechanism, as well as its preparation method and application. The fluorescent probe is: 4-(2-(4-(cyclopropylformyloxy-4′-(6,6-dimethyl-2-(4-nitrophenyl)-4,5,6,7-tetrahydro-2H-5,7-methyleneindazole-3-yl)-[1,1′-biphenyl]-3-yl)vinyl)-1-methylpyridinium iodide (abbreviated as: THIP-OCP). The compound can undergo enzymatic hydrolysis reaction with butyrylcholinesterase and, under irradiation with ultraviolet light at a wavelength of 365nm, The solution's fluorescence color changes from colorless to orange-yellow. Since methyl parathion effectively inhibits butyrylcholinesterase activity, when methyl parathion is added to the solution, the solution's fluorescence gradually changes from orange-yellow to colorless under ultraviolet irradiation at a wavelength of 365 nm. Therefore, this compound can be used as a fluorescent probe for detecting methyl parathion. This compound has many advantages, including a low detection limit (0.79 μg / mL) and a wide pH range (5-10), and has great application prospects.

The invention discloses a UiO-66-NH2 (at) MWCNTs (at) CeO2 composite material, a preparation method thereof and a method for preparing an electrochemical sensor, and particularly relates to the technical field of pesticide residue detection. The core UiO-66-NH2 (at) MWCNTs (at) CeO2 composite material provided by the invention is prepared from the following components: UiO-66-NH2, MWCNTs and CeO2, and the mass ratio of the UiO-66-NH2 to the MWCNTs to the CeO2 is (2 to 10): 1: (1 to 5). The invention provides a UiO-66-NH2 (at) MWCNTs (at) CeO2 composite material, a preparation method of the UiO-66-NH2 (at) MWCNTs (at) CeO2 composite material and a method for preparing an electrochemical sensor. According to the research, through experimental optimization, an electrochemical sensing platform based on the UiO-66-NH2 (at) MWCNTs (at) CeO2 ternary composite material is successfully constructed, and high-performance synchronous detection of typical pollutants such as cadmium, parathion-methyl and mercury in a sample to be detected is realized.

The present application relates to the technical field of microbial pesticide pollution remediation, and specifically discloses a strain capable of efficiently degrading MCPA and application thereof. The strain is Priestia megaterium AS, and the preservation number is CCTCC No. M 20242160. The present application provides a method for isolating and screening the strain, which grows well in a culture medium containing 500 μmol / L MCPA and can degrade MCPA as a carbon source, with a degradation rate of 500 μmol / L MCPA exceeding 50% within 7 days. It is proved by a potting test that inoculation of the strain or its preparation can significantly reduce the MCPA residue in the rhizosphere soil, effectively alleviate the toxic effect of MCPA on sensitive crops such as melons and pumpkins, and promote the growth recovery of plants. The strain and its bacterial agent provide an efficient and environmentally friendly biological technology solution for remediation of MCPA contaminated soil and guarantee of safe production of melon crops.

This invention belongs to the field of food and agricultural product safety testing technology, specifically relating to a rapid detection method for parathion and its application. Based on the parathion-induced blue shift in fluorescence of silicon nanoparticles, ascorbic acid is reacted with 3-aminopropyltriethoxysilane to generate green fluorescent silicon nanoparticles. During the formation of silicon nanoparticles, parathion can embed itself into the porous structure of the silicon nanoparticles through host-guest interactions, causing a change in the excited state of the silicon nanoparticles. This affects the fluorescence emission of the silicon nanoparticles, resulting in a blue shift in the fluorescence emission peak, changing from green fluorescence to blue fluorescence, specifically manifested as an increase in fluorescence intensity at 422 nm and a decrease in fluorescence intensity at 520 nm. By establishing the relationship between ratiometric fluorescence signal or fluorescence color change and parathion concentration, specific detection of parathion is achieved. This invention requires no enzymes or antibodies for parathion detection, is inexpensive, fast, easy to operate, highly sensitive, and has good specificity.

The invention belongs to the technical field of photocatalysis, and discloses a preparation method of a visible light region LSPR (Localized Surface Plasmon Resonance) catalyst for absorbing and degrading organophosphorus pesticides. According to the invention, a plasma semiconductor MoO3-x is used as a precursor, shows a strong LSPR effect under incident light, and has high charge carrier density and large specific surface area. Sulfides such as sulfur indium zinc, sulfur indium cadmium and the like are loaded on the surface of the MoO3-x nanosheet through in-situ synthesis. The catalyst forms a Z-type heterojunction structure, not only can preserve photo-generated electrons with high reduction ability, but also can preserve photo-generated holes with high oxidation ability, effectively separates electron-hole pairs, and expands the photoresponse range. The method is suitable for degrading various organophosphorus pesticides such as amidothion, parathion-methyl and the like. The composite material is low in cost, simple to prepare, rich in active sites, large in specific surface area, high in degradation rate and good in stability, and the visible light utilization rate is increased by virtue of the LSPR effect.

The present invention provides a methyl parathion hapten, a sibutramine hapten, a methyl parathion-sibutramine artificial antigen and antibody, and a detection kit, relating to the technical field of food safety detection. The polyclonal antibody obtained by immunization with the artificial antigen not only has high sensitivity but also can simultaneously identify both methyl parathion and sibutramine, thus solving the technical problem of the lack of a highly sensitive and rapid method for the simultaneous detection of methyl parathion and sibutramine in the prior art. The half inhibitory concentration (IC50) of the antibody for methyl parathion is 100%. 50 ) was 4.59 ng / mL, the quantitative detection range was 2.89 ng / mL-7.28 ng / mL, and the detection limit was 2.21 ng / mL; the half-inhibitory concentration (IC 50 ) was 59.76 ng / mL, the quantitative detection range was 37.65 ng / mL-94.87 ng / mL, and the detection limit was 28.73 ng / mL.

This invention relates to the field of pollutant detection technology, and discloses a method for preparing bimetallic selenides and its non-enzymatic electrochemical sensor and applications. The reference electrode of the non-enzymatic electrochemical sensor is a silver / silver chloride electrode, the auxiliary electrode is a platinum wire electrode, and the working electrode is a glassy carbon composite electrode containing a bimetallic selenide coating. The bimetallic selenide is CuInSe2, which exhibits sensitivity in identifying parathion. This improves the accuracy and ease of operation in detecting parathion and reduces the detection cost. The method for preparing the bimetallic selenide proposed in this invention uses ethylamine and N,N-dimethylformamide as solvents, and through a solvothermal reaction, reacts cuprous chloride, indium chloride, and selenium powder to generate CuInSe2, which has the advantages of high reaction efficiency and low manufacturing cost.

This invention belongs to the field of pesticide residue detection technology, specifically relating to a method for preparing a cerium-based covalent organic framework composite material with alkaline phosphatase activity and its application. Using COF, L-valine, and Ce(NO3)3·6H2O as raw materials, a COF-OMe@Valine-CeO2 nanozyme was prepared via in-situ growth. This nanozyme exhibits alkaline phosphatase activity, and the constructed sensor demonstrates good selectivity, repeatability, and stability. The preparation method of the CeO2-Val@COF-OMe nanozyme is simple and can achieve rapid detection and hydrolysis of the organophosphorus pesticide methyl parathion.

This invention discloses a ratiometric electrochemical sensor, its preparation method, and a method for simultaneously detecting methyl parathion and carbendazim. The sensor uses a CeCoZn-MOF / NBA / CNHs-modified glassy carbon electrode as the working electrode, a saturated KCl-type Ag / AgCl as the reference electrode, and a platinum wire as the counter electrode. Preparation involves electrode pretreatment, CeCoZn-MOF synthesis, composite material compounding, dispersion preparation, and electrode drop-coating modification. Differential pulse voltammetry is used for detection, with Nile Blue A as the internal standard probe. Quantification is based on the current ratio of the target analyte to the internal standard. The linear detection range for methyl parathion is 0.05–15 μg / mL, and for carbendazim, it is 0.05–4 μg / mL, with correlation coefficients greater than 0.99 for both. This sensor exhibits strong anti-interference capabilities, high sensitivity and accuracy, with a recovery rate of 91.0%–109.2% in actual agricultural product samples. It enables rapid, simultaneous, and accurate detection of methyl parathion and carbendazim, and is suitable for rapid on-site detection of pesticide residues in agricultural products.

The present invention discloses an affinity-matured anti-parathion nanobody and its application. The present invention performs mutation on the anti-parathion nanobody Nb9 to obtain a nanobody mutant H6 with higher affinity for parathion, the amino acid sequence of which is shown in SEQ ID NO.1. The nanobody can specifically recognize parathion pesticides, and its sensitivity is 2.9 times higher than that of nanobody Nb9, and the minimum detection limit is reduced by 2.6 times; it is also better than the sensitivity of other positive mutants identified, and its thermal stability and tolerance to organic solvents are improved. It is further used to detect parathion pesticides, and its detection IC 50 The detection limit is 3.55 ng / mL, the minimum detection limit is 1.30 ng / mL, and the linear range is 1.89-6.67 ng / mL. The detection results are accurate and stable, and it can be more widely used in the detection of parathion pesticide residues in agricultural products.

The invention discloses a preparation method of a Ce-MOF / CNHs (at) NBA / GCE electrode, an electrochemical sensor and application of the electrochemical sensor in detection of parathion-methyl. The preparation method comprises the following steps: sequentially polishing a glassy carbon electrode GCE to be smooth by using aluminum oxide powder with different particle sizes, sequentially carrying out ultrasonic treatment in ethanol and pure water to remove surface residues, and airing at room temperature; the preparation method comprises the following steps: adding single-walled carbon nanohorns (CNHs) powder and Nile blue A (NBA) powder into an aqueous solution, uniformly stirring at room temperature, centrifuging, washing and drying to obtain CNHs (at) NBA powder, preparing a mixed solution containing benzenetricarboxylic acid H3BTC and CNHs (at) NBA, adding cerous nitrate hexahydrate Ce (NO3) 3.6 H2O powder, stirring again, centrifuging, washing and drying to obtain a Ce-MOF / CNHs (at) NBA compound; the Ce-MOF / CNHs (at) NBA compound is dispersed in pure water, and a Ce-MOF / CNHs (at) NBA solution with the concentration being 0.2 mg / mL to 1 mg / mL is obtained; and dropwise adding a Ce-MOF / CNHs (at) NBA solution to the glassy carbon electrode GCE treated in the step (1), and airing at room temperature to obtain the Ce-MOF / CNHs (at) NBA / GCE electrode.

The present application belongs to the technical field of pesticide detection, and particularly relates to a multi-in-one pesticide residue quantitative detection test paper and a detection method. The test paper is provided with gold nanoparticle labeled antibodies of at least two kinds of pesticides on a colloidal gold combination pad; a chromatography membrane is provided with a quality control line and at least two detection lines, each detection line is coated with an antigen of one kind of pesticide; the pesticides are selected from chlorpyrifos, phorate, dimethoate, acetamiprid, carbendazim, procymidone, fenpropathrin, carbofuran, isoprocarb, chlorothalonil, chlorfenapyr, parathion, tetramethylenedisulfide, triadimefon, isocarbophos or parathion. Through optimization of the pesticide types and preparation methods of the antigens and antibodies, the present application overcomes the problem of mutual interference of detection targets when multiple pesticides are simultaneously detected, has good specificity, high sensitivity, can accurately quantify, can simultaneously detect multiple components, and has simple pretreatment, and thus has good application prospect.