31 results about "Gold marker" patented technology

The invention relates to the technical field of biology, in particular to an antigen chip used for detecting human parasites antigens in serum and a preparation method thereof. The invention spots antigen array of parasites which are seriously harmful to human body, Schistosoma, Lung fluke, Clonorchis sinensis, Cysticercosis, Sparganum mansoni, Trichina, Angiostrongylus cantonensis, Toxoplasma gondii, and the like, on a solid phase membrane carrier, and specific gamma immunoglobulin (IgG) of human parasites in serum is detected by a specially made vertical flow chip detect device, and the detecting marker is colloid gold second antibody conjugate or colloid gold protein A conjugate of fresh color. IgG antibodies in serum specially bind with the antigens on the membrane in percolation process layer upon layer, and human IgG which specially binds with the antigens is detected by the colloid gold marker. Detecting of a plurality of parasites antigen indexes in serum sample can be finished within several minutes. The device and the method have the advantages of high throughput, simple operation, convenient use and being suitable for parasitic diseases clinical test and serum-epidemiological investigation.

The invention discloses a test paper card for detecting aflatoxin B1 and application of the test paper card. The test paper card comprises a sample absorption pad, a conjugate release pad, a reactive film, a water absorption pad and a bottom plate, wherein a detection line coated with an aflatoxin B1 hapten-carrier protein conjugate and a quality control line coated with a goat anti-rat antibody are arranged on the reactive film; an aflatoxin B1 monoclonal antibody-colloid gold marker is sprayed on the conjugate release pad. The invention also provides a method for detecting aflatoxin B1 residues in grains and feed by using the aflatoxin B1 test paper card. The test paper card provided according to the invention has the characteristics of simplicity in operation, high sensitivity, high detection speed, low cost and the like and is suitable for screening and field monitoring of lots of samples.

The invention discloses a test strip for detecting estriol and applications thereof. The test strip comprises a sample absorbing pad (1), a combined substance releasing pad (2), a reaction membrane (3), a water absorbing pad (4), and a bottom plate (7). The reaction membrane comprises a detection line (5) embedded with an estriol semi-antigen-carrier protein conjugate and a quality control line (6) embedded with a goat-anti-mouse anti-antibody. An estriol monoclonal antibody-colloid gold marker is sprayed on the combined substance releasing pad (2). The invention also provides a method using the provided estriol test strip to detect residual estriol in fish meat, chicken, shrimp meat, and pork. The provided test strip has the characteristics of simple operation, high sensitivity, fast detection speed, and low cost, and is suitable for screening of massive samples and onsite monitoring.

The invention provides a cobalt-zirconium gold marker implant, and relates to the technical field of implanted medical apparatuses. The cobalt-zirconium gold marker implant comprises a gold marker main body, wherein the gold marker main body is made from metallic cobalt and zirconium; the gold marker main body is represented in a capsule-shaped or drum-shaped form, and lines, by which friction is increased, are arranged on the outer surface of the gold marker main body; and a drug layer is additionally arranged on the outer surface of the gold marker main body. Before a tumor treatment operation, the cobalt-zirconium gold marker implant is implanted into a focus or surrounding tissues thereof; with the design of the lines, friction force between the cobalt-zirconium gold marker implant and human tissues is increased, and since the metallic cobalt and zirconium can shelter X light, the cobalt-zirconium gold marker implant is helpful for medical personnel to accurately track tumor locations in a process of implementing radiotherapy, so that purposes of rapid location and precise treatment are achieved; meanwhile, high-purity drug molecules, which are released from the drug layer, are directly absorbed by the focus so as to take an adjuvant therapy effect to inhibit or kill tumor cells and to improve operating and therapeutic effects. On this basis, the invention also provides a gold marker implantation apparatus.

The invention provides atest strip for detecting pendimethalin, and a preparation method and application thereof. The test strip comprises a sample absorbing cushion, a conjugate releasing cushion, areacting film, a water absorbing cushion and a base plate and is characterized in that the reacting film is provided with detection lines coated with pendimethalin hatpin-carrier protein couplers andquality control lines coated with anti-goat and anti-mouse anti-bodies, and pendimethalin monoclonal antibody-colloid gold markers are sprayed on the conjugate releasing cushion. The pendimethalin hatpin is prepared through the steps that N-(1-ethyl propyl ether)-3,4-methyl toluidine reacts with 4-ethyl 4-bromobutyrate to generate hydrocarbylation N-(1-ethyl propyl ether)-3,4-methyl toluidine, then the hydrocarbylation N-(1-ethyl propyl ether)-3,4-methyl toluidine reacts with concentrated nitric acid to generate nitro hydrocarbonylation N-(1-ethyl propyl ether)-3,4-methyl toluidine, and then the nitro hydrocarbonylation N-(1-ethyl propyl ether)-3,4-methyl toluidine reacts with KOH. The test striphas the advantages of being easy and convenient to operate, high in sensitivity, high in detecting speed, low in cost, and not limited by detecting equipment and can conduct rapid detection and on-site monitoring on great batches of pendimethalin samples.

The invention relates to an extracorporal test kit for TAFI content, and a test method of the extracorporal test kit. The extracorporal test kit for the TAFI content consists of a reagent, a TAFI standard product and a TAFI quality control product, wherein the reagent contains a monoclonal antibody nano-gold marker R that is specifically bonded with TAFI; the TAFI standard product and TAFI quality control product are used for making a standard curve for calculating the content of a TAFI antigen in a to-be-tested sample. The biological characteristic that nano-gold is bonded with an antibody, and a conventional biochemical test method is combined, so that the extracorporal test method of the extracorporal test kit is used for an extracorporal test of the content of the TAFI, a new extracorporal diagnostic method is developed for the TAFI, and the sensitivity of the extracorporal test of the TAFI is improved. According to the extracorporal test method, the problem that the equipment is expensive in the prior art is solved, the detectable rate and the accuracy rate on relevant diseases are improved, unnecessary pains and medical cost of a tested person are reduced, and the living quality of the tested person is improved.

The invention provides an immunochromatography detection system. The system comprises a light source for detection, a standard light source, a photoelectric detector, and a piece of test paper for detection; the test paper for detection is arranged in a detection area, a detection line is arranged in the detection area, when a detection target exists in a sample, an immunochromatography reaction is carried out for nano-gold marker particles, and the particles are aggregated on the detection line; the light source for detection and the standard light source can respectively emit detection light and reflected light to the test paper for detection; the photoelectric detector can receive the reflected light in the detection area; and the test paper for immunochromatography detection is provided with a calibration area, the calibration area is provided with a calibration color block, the calibration color block is positioned in a color developing range of the nano-gold marker particles, a detection result is corrected by means of irradiation on the calibration color block by the standard light and the light for detection, and whether further correction is needed is determined.

The invention provides test paper for detecting progesterone of an estrous female dog and a preparation method of the test paper. The technical scheme is based on a colloid gold immunochromatography and adopts a competition method, an improved progesterone antigen composition and goat (rabbit) anti-mouse IgG are fixed on a nitrocellulose membrane (NC) in a strip form, an anti-progesterone monoclonal antibody-colloid gold marker is fixed on a combination pad, when a sample to be detected is loaded to a test strip sample loading hole, the sample to be detected moves forwardly by virtue of the effect of a capillary tube, after the colloid gold marker on the combination pad is dissolved, the sample to be detected has inter-reaction and then is moved to an area for fixing an antigen or antibody, the sample to be detected is specifically combined with the colloid gold marker to be intercepted and accumulated onto a detection strip, and a developing result can be observed by eyes. The test paper is simple and rapid in detection, free from complicated operation skills and special devices and convenient to carry; and in addition, the test paper can realize semi-quantitative analysis for theprogesterone, is sensitive and accurate in result, and is more objective and effective for evaluating the estrous situation of the female dog.

The invention belongs to the technical field of medical treatment, and particularly discloses an intrahepatic portosystem shunt stent with the diameter capable of being automatically adjusted. The intrahepatic portosystem shunt stent with the diameter capable of being automatically adjusted comprises an intrahepatic membrane segment, a non-projection line gold marker line, a portal bare segment and a through hole groove, wherein a right side end of the intrahepatic membrane segment is fixedly connected with the non-projection line gold marker line, a right side end of the non-projection line gold marker line is fixedly connected with the portal bare segment, a dilation head is fixedly installed at a right side end of the portal bare segment, multiple overhead hooks are evenly and fixedly installed on an outer wall of the dilation head, an inner membrane dilation opening is fixedly formed in an inner wall of the dilation head, and the through hole groove is formed in both inner walls ofthe intrahepatic membrane segment and the portal bare segment. The intrahepatic portosystem shunt stent with the diameter capable of being automatically adjusted has the comprehensive effects that the intrahepatic portosystem shunt stent with the diameter capable of being automatically adjusted can be effectively and steadily connected with a blood vessel, and is not liable to fall off, a successrate of installation is high, and meanwhile, the insertion depth can be observed conveniently.

The invention discloses antibiotics detection test paper. The antibiotics detection test paper comprises a sample absorption pad, a gold marker combination pad, a reaction film and absorption paper, wherein the gold marker combination pad is coated with a beta-lactam specific antibody-colloidal gold marker, a tetracycline specific antibody-colloidal gold marker, a quinolone specific antibody-colloidal gold marker, a macrolide specific antibody-colloidal gold marker, a sulfanilamide specific antibody-colloidal gold marker and a cephalosporin specific antibody-colloidal marker; the reaction film is coated with a macrolide antibiotics detection line, a beta-lactam antibiotics detection line, a tetracycline antibiotics detection line, a quinolone antibiotics detection line, a sulfanilamide antibiotics detection line, a cephalosporin antibiotics detection line and a goat-anti-mouse quality control line. The antibiotics detection test paper can realize combined detection of various antibiotics in urine of children, can be conveniently and quickly used without professional operation, and is suitable for household use.

The invention provides an immune colloidal gold test paper card for testing spiramycin. The test paper card comprises a reaction membrane, a sample pad, a gold marker pad, a water absorption pad and aPVC sheet, the reaction membrane has a testing area coated with a spiramycin hapten-carrier protein conjugate and a quality control area coated with goat anti mouse IgG, a conjugate releasing pad iscoated with a monoclonal spiramycin antibody-colloidal gold marker. The invention further provides a method utilizing the above-mentioned test paper card for testing spiramycin. The method comprises the steps that firstly, a sample is pretreated, then the pretreated sample is tested by the test paper card, and finally, the testing result is analyzed. The test paper card can be used for testing theamount of residual spiramycin medicine in animal-derived food such as chicken and feed, and is simple in operation, high in sensitivity, high in testing speed, low in cost, and capable of achieving on-site supervision and meeting the requirements for screening a large number of samples.

A removable dual gold marker implantation device with minor damage comprises a gold marker and a puncture needle, the gold marker is arranged in the needle barrel of the puncture needle when in use; the gold marker comprises a gold marker head and a connecting wire connected with the gold marker head; the gold marker head comprises a first gold marker, a connecting part and a second gold marker connected in sequence. The disclosure reduces the damage to the body when removing the gold marker, and the dual gold marker structure makes the implantation of multiple gold markers at the same time possible and reduces the number of times of implantation and improves the efficiency.

The invention discloses a detection method for 1-aminohydantoin hydrochloride in animal tissues and a detection card. The detection card and a reagent are provided, wherein the surface of the detection card is provided with a colloidal gold test strip; the inner side of an ELIASA hole is provided with a monoclonal colloid gold marker antibody of frozen 1-aminohydantoin hydrochloride; the top of the surface of the detection card is provided with comparison color blocks at equal intervals; the reagent comprises deionized water, 1M hydrochloric acid, a derivatization reagent, an extraction agent, 1M sodium hydroxide, gold chloride, double distilled water, a chloroauric acid solution, a 1-percent sodium citrate aqueous solution, 0.1mol/L K2CO2 and a 0.1mol/L HCL cloning solution. In the detection method, the monoclonal colloid gold marker antibody is used, so that covalent bonds between gold particles and antibody molecules are avoided in the monoclonal colloid gold marker antibody, and the metal particles and the antibody molecules are combined through Van der Waals force among charges of different polarities; the colloid gold marker has very small influences on the specificity and affinity of the antibody, so that the detection accuracy of the 1-aminohydantoin hydrochloride can be increased.

The invention provides an immune colloidal gold test paper card for testing diazepam. The test paper card comprises a reaction membrane, a sample pad, a gold marker pad, a water adsorption pad and a back sheet, the reaction membrane has a test area coated with a diazepam hapten-carrier protein conjugate and a quality control area coated with goat anti mouse IgG, and a conjugate releasing pad is coated with a diazepam monoclonal antibody-colloidal gold marker. The invention further provides a method utilizing the test paper card for testing diazepam. The method comprises the steps that firstly,a sample is pretreated, then the pretreated sample is tested by the test paper card, and finally, the test result is analyzed. The test paper card can be used for testing the amount of residual diazepam medicine in animal-derived food and urine samples, and is simple in operation, high in sensitivity, high in testing speed, low in cost, and capable of achieving on-site supervision and meeting therequirements for screening a large number of samples.