31 results about "Gold marker" patented technology

The invention relates to the technical field of biology, in particular to an antigen chip used for detecting human parasites antigens in serum and a preparation method thereof. The invention spots antigen array of parasites which are seriously harmful to human body, Schistosoma, Lung fluke, Clonorchis sinensis, Cysticercosis, Sparganum mansoni, Trichina, Angiostrongylus cantonensis, Toxoplasma gondii, and the like, on a solid phase membrane carrier, and specific gamma immunoglobulin (IgG) of human parasites in serum is detected by a specially made vertical flow chip detect device, and the detecting marker is colloid gold second antibody conjugate or colloid gold protein A conjugate of fresh color. IgG antibodies in serum specially bind with the antigens on the membrane in percolation process layer upon layer, and human IgG which specially binds with the antigens is detected by the colloid gold marker. Detecting of a plurality of parasites antigen indexes in serum sample can be finished within several minutes. The device and the method have the advantages of high throughput, simple operation, convenient use and being suitable for parasitic diseases clinical test and serum-epidemiological investigation.

A method for detecting total IgG combined with Fc fragment of Staphylococci protein A (SPA) in mammalian serum with colloidal gold-labeled protein A comprises the following steps of: 1) construction of a standard curve and calculation of regression equation for each batch of colloidal gold-labeled probe and micro-reaction by (1) setting 8 standard holes, adding 50MuL 0.01M TBS solution (pH 7.4, containing 0.1% calf serum) to each hole; (2) adding 50MuL standard IgG solution from the first hole to the sixth hole by doubling dilution, sucking out 50MuL TBS solution from the seventh hole, adding 50MuL standard IgG solution, and leaving the eighth hole as a blank control; (3) adding 50MuL colloidal gold-labeled probe diluted by 2-6 times in the reaction holes; (4) completely mixing the reaction plate, and reacting at room temperature for 20-40min; (5) detecting absorbance at 620nm with a microplate-reader for ELISA; and (6) calculating common logarithm of the absorbance associated with the standard IgG; and 2) sample detection by (1) adding 50MuL sample solution in the sample holes; (2) adding 50MuL diluted colloidal gold-labeled probe in the sample holes; (3) completely mixing the reaction plate, and reacting at room temperature for 20-40min; and (4) detecting absorbance of each hole at 620nm with the microplate-reader for ELISA, and calculating corresponding IgG values according to the regression equation of the standard curve.

The invention belongs to a collaurum immunochromatography test strip detecting mycoplasma pneumoniae, comprising a sample pad, a combining pad, a nitrocellulose coating film, a water absorption pad and a PVC bottom plate; the sample pad, the combining pad, the nitrocellulose coating film and the water absorption pad are overlapped on the PVC bottom plate in sequence; the combining pad is coated with a mycoplasma pneumoniae antibody MPh2-collaurum-carbon nanotube marker; the nitrocellulose coating film is provided with a detection line and a quality control line; the detection line is coated with a mycoplasma pneumoniae antibody MPh1 and the quality control line is coated with a goat anti mouse IgG. The invention further comprises a preparing method of the collaurum immunochromatography test strip, comprising the steps of preparing of collaurum, preparing of a gold marker antibody, purifying and assembling of the test strip. The collaurum immunochromatography test strip has the characteristics of high specificity, high sensitivity, simple and convenient operation, fast detection, accuracy and suitability for field use.

The invention belongs to the field of bio-detection and particularly relates to a C reactive protein saliva test paper strip and a preparation method thereof. The C reactive protein saliva test paper strip includes a reactive film and a gold marker pad. The reactive film is a nitrocellulose membrane, which has a detection line coated with a C reactive protein monoclonal antibody and a quality control line coated with goad-anti-mouse IgG. The gold marker pad is a polyester membrane that is coated with the C reactive protein monoclonal antibody marked by colloidal gold. The test paper strip, with human saliva as a detection sample, can effectively monitor the C reactive protein in saliva. The test paper strip has strong specificity and good repeatability, has high sensitivity, is 10 mg / L in lowest detection limit, is easy to operate and is free of special instruments and devices and professional training, has clear and distinguishable result and is easy to promote, and is suitable for on-site test.

The invention relates to a binary detection test strip of beta-exhilarant ractopamine and salbutamol and a preparation method thereof, which can effectively solve the problem that the beta-exhilarant ractopamine and salbutamol can not be detected quickly, easily and simultaneously. The preparation method comprises the following steps of: arranging diversion glass fiber, carrier glass fiber, a nitrocellulose membrane and an absorbent cotton pulp board a on a substrate PVC (Polyvinyl Chloride) board from back to front in order; covering a coating comprising a front coating and a rear coating onthe diversion glass fiber, the carrier glass fiber and the absorbent cotton pulp board, wherein a monoclonal antibody for resisting ractopamine protein conjugates and a monoclonal antibody colloid gold marker for resisting salbutamol protein conjugates are adsorbed in the carrier glass fiber; and coating the nitrocellulose membrane with a quality control line and two test lines, wherein the quality control line is coated with rabbit antimouse multi-resistant, and the test lines are coated with ractopamine protein conjugates and salbutamol protein conjugates. The invention can be used for detecting the beta-exhilarant ractopamine and the salbutamol simultaneously, effectively, conveniently and quickly and has accurate result.

The invention discloses a test strip for detecting estriol and applications thereof. The test strip comprises a sample absorbing pad (1), a combined substance releasing pad (2), a reaction membrane (3), a water absorbing pad (4), and a bottom plate (7). The reaction membrane comprises a detection line (5) embedded with an estriol semi-antigen-carrier protein conjugate and a quality control line (6) embedded with a goat-anti-mouse anti-antibody. An estriol monoclonal antibody-colloid gold marker is sprayed on the combined substance releasing pad (2). The invention also provides a method using the provided estriol test strip to detect residual estriol in fish meat, chicken, shrimp meat, and pork. The provided test strip has the characteristics of simple operation, high sensitivity, fast detection speed, and low cost, and is suitable for screening of massive samples and onsite monitoring.

The invention provides a test strip, and belongs to the field of food safety fast detection. The test strip comprises a baseplate, a sample cushion, a conjugate release cushion, a reaction film and a water-absorbing cushion, wherein the sample cushion, the conjugate release cushion, the reaction film and the water-absorbing cushion are adhered onto the baseplate and in tight contact with one another sequentially; part of the conjugate release cushion is coated with the sample cushion; the reaction film is coated with detection lines made of amantadine-carrier protein conjugate and quality control lines made of goat anti-mouse IgG; the conjugate release cushion is coated with a colloid gold marker made of amantadine. The test strip has the advantages that the observing time of a detection result can be prolonged; the sample absorption cushion can sufficiently absorb a detection solution and be in full contact with a gold-labelled antibody for full reaction, and then chromatography is conducted on the reaction film for reaction, so that error can be reduced effectively. The test strip can be used for further preventing protein in the gold-labelled antibody from losing activity under the action of certain interference ingredients in a detection sample, so that the impact on the combination of the gold-labelled antibody and a coating antigen is avoided.

The invention provides a test strip for detecting acetamiprid, and a preparation method and application thereof and further provides a method using the test strip to detect the acetamiprid. The test strip comprises a sample absorbing cushion, a conjugate releasing cushion, a reacting film, a water absorbing cushion and a base plate. The reacting film is provided with detection lines coated with acetamipridhatpin-carrier protein couplers and quality control lines coated with anti-goat and anti-mouse anti-bodies, and acetamipridmonoclonal antibody-colloid gold markers are sprayed on the conjugate releasing cushion. Acetamiprid monoclonal antibodies are prepared with the acetamiprid hatpin-carrier protein couplers as immunogen, the acetamiprid hatpin-carrier protein couplers are prepared through the method that acetamiprid hatpin and acarrier protein are coupled, and the acetamiprid hatpin is obtained through the reaction of N-nor-acetamiprid and aminobutyric acid. The test strip and thedetection method have the advantages that operation is easy, the sensitivity is high, the detecting speed is high, the cost is low, limitation from detecting equipment is avoided, and can conduct rapid detection and on-site monitoring on the acetamiprid in great batches of samples.

The invention relates to the field of biological detection and particularly relates to a detection kit for quantitatively detecting golgi apparatus glycoprotein-73GP73 as well as a preparation method and application thereof. The detection kit for quantitatively detecting the golgi apparatus glycoprotein-73GP73, provided by the invention, comprises a test paper card, wherein the test paper card comprises a bottom plate, and a sample pad, a gold marker pad, a nitrocellulose membrane and a water suction pad which are located on the surface of the bottom plate and are sequentially arrayed from a feeding end; the gold marker pad contains a golgi apparatus glycoprotein-73GP73 antibody; the nitrocellulose membrane is coated with a detection line and a quality control line; and the golgi apparatus glycoprotein-73GP73 antibody on the gold marker pad is marked by fluorescent microspheres. According to the detection kit provided by the invention, the golgi apparatus glycoprotein-73GP73 is detected by a fluorescent microsphere immunochromatography technology for the first time, and the detection kit has sensitivity and specificity; and the detection kit has the advantages of rapidness and convenience for operation, accurate result, affordable property and the like.

The invention provides a cobalt-zirconium gold marker implant, and relates to the technical field of implanted medical apparatuses. The cobalt-zirconium gold marker implant comprises a gold marker main body, wherein the gold marker main body is made from metallic cobalt and zirconium; the gold marker main body is represented in a capsule-shaped or drum-shaped form, and lines, by which friction is increased, are arranged on the outer surface of the gold marker main body; and a drug layer is additionally arranged on the outer surface of the gold marker main body. Before a tumor treatment operation, the cobalt-zirconium gold marker implant is implanted into a focus or surrounding tissues thereof; with the design of the lines, friction force between the cobalt-zirconium gold marker implant and human tissues is increased, and since the metallic cobalt and zirconium can shelter X light, the cobalt-zirconium gold marker implant is helpful for medical personnel to accurately track tumor locations in a process of implementing radiotherapy, so that purposes of rapid location and precise treatment are achieved; meanwhile, high-purity drug molecules, which are released from the drug layer, are directly absorbed by the focus so as to take an adjuvant therapy effect to inhibit or kill tumor cells and to improve operating and therapeutic effects. On this basis, the invention also provides a gold marker implantation apparatus.

The invention provides atest strip for detecting pendimethalin, and a preparation method and application thereof. The test strip comprises a sample absorbing cushion, a conjugate releasing cushion, areacting film, a water absorbing cushion and a base plate and is characterized in that the reacting film is provided with detection lines coated with pendimethalin hatpin-carrier protein couplers andquality control lines coated with anti-goat and anti-mouse anti-bodies, and pendimethalin monoclonal antibody-colloid gold markers are sprayed on the conjugate releasing cushion. The pendimethalin hatpin is prepared through the steps that N-(1-ethyl propyl ether)-3,4-methyl toluidine reacts with 4-ethyl 4-bromobutyrate to generate hydrocarbylation N-(1-ethyl propyl ether)-3,4-methyl toluidine, then the hydrocarbylation N-(1-ethyl propyl ether)-3,4-methyl toluidine reacts with concentrated nitric acid to generate nitro hydrocarbonylation N-(1-ethyl propyl ether)-3,4-methyl toluidine, and then the nitro hydrocarbonylation N-(1-ethyl propyl ether)-3,4-methyl toluidine reacts with KOH. The test striphas the advantages of being easy and convenient to operate, high in sensitivity, high in detecting speed, low in cost, and not limited by detecting equipment and can conduct rapid detection and on-site monitoring on great batches of pendimethalin samples.

The invention belongs to the field of bio-detection and particularly relates to a C reactive protein saliva test paper strip and a preparation method thereof. The C reactive protein saliva test paper strip includes a reactive film and a gold marker pad. The reactive film is a nitrocellulose membrane, which has a detection line coated with a C reactive protein monoclonal antibody and a quality control line coated with goad-anti-mouse IgG. The gold marker pad is a polyester membrane that is coated with the C reactive protein monoclonal antibody marked by colloidal gold. The test paper strip, with human saliva as a detection sample, can effectively monitor the C reactive protein in saliva. The test paper strip has strong specificity and good repeatability, has high sensitivity, is 10 mg / L in lowest detection limit, is easy to operate and is free of special instruments and devices and professional training, has clear and distinguishable result and is easy to promote, and is suitable for on-site test.

The invention relates to an extracorporal test kit for TAFI content, and a test method of the extracorporal test kit. The extracorporal test kit for the TAFI content consists of a reagent, a TAFI standard product and a TAFI quality control product, wherein the reagent contains a monoclonal antibody nano-gold marker R that is specifically bonded with TAFI; the TAFI standard product and TAFI quality control product are used for making a standard curve for calculating the content of a TAFI antigen in a to-be-tested sample. The biological characteristic that nano-gold is bonded with an antibody, and a conventional biochemical test method is combined, so that the extracorporal test method of the extracorporal test kit is used for an extracorporal test of the content of the TAFI, a new extracorporal diagnostic method is developed for the TAFI, and the sensitivity of the extracorporal test of the TAFI is improved. According to the extracorporal test method, the problem that the equipment is expensive in the prior art is solved, the detectable rate and the accuracy rate on relevant diseases are improved, unnecessary pains and medical cost of a tested person are reduced, and the living quality of the tested person is improved.

The invention provides an immunochromatography detection system. The system comprises a light source for detection, a standard light source, a photoelectric detector, and a piece of test paper for detection; the test paper for detection is arranged in a detection area, a detection line is arranged in the detection area, when a detection target exists in a sample, an immunochromatography reaction is carried out for nano-gold marker particles, and the particles are aggregated on the detection line; the light source for detection and the standard light source can respectively emit detection light and reflected light to the test paper for detection; the photoelectric detector can receive the reflected light in the detection area; and the test paper for immunochromatography detection is provided with a calibration area, the calibration area is provided with a calibration color block, the calibration color block is positioned in a color developing range of the nano-gold marker particles, a detection result is corrected by means of irradiation on the calibration color block by the standard light and the light for detection, and whether further correction is needed is determined.

The invention relates to a triazophos monoclonal antibody. The triazophos monoclonal antibody is characterized in that the amino acid sequence of a heavy-chain variable region of the antibody is shownas SEQID NO:1, and the amino acid sequence of a light-chain variable region of the antibody is shown as SEQID NO:2. The invention relates to an application of the triazophos monoclonal antibody to colloidal gold detection test paper. The colloidal gold detection test paper comprises a liner, as well as a sample pad, a gold marker combination pad, a cellulose membrane and a water absorption pad which are sequentially arrayed on the liner, wherein the inner part of the gold marker combination pad is adsorbed with a triazophos gold marker antibody, a detection line and a control line are formedin the cellulose membrane, the detection line is printed with a triazophos hapten coupled carrier protein solution, carrier protein adopted in the carrier protein solution is prepared through couplingO-ethyl-O-(1-phenyl-1,2,4-triazole-3-based)-N-(butyric acid based) thiophosphate ester with bovine serum albumin (BSA) in an active ester method, and the control line is printed through a rabbit antimouse IgG antibody. The triazophos monoclonal antibody disclosed by the invention is high in specificity, high in sensibility, simple, convenient and quick to detect, vivid, audio-visual and accuratein result display, wide in application range and convenient to popularize, and fees are saved.

The invention provides test paper for detecting progesterone of an estrous female dog and a preparation method of the test paper. The technical scheme is based on a colloid gold immunochromatography and adopts a competition method, an improved progesterone antigen composition and goat (rabbit) anti-mouse IgG are fixed on a nitrocellulose membrane (NC) in a strip form, an anti-progesterone monoclonal antibody-colloid gold marker is fixed on a combination pad, when a sample to be detected is loaded to a test strip sample loading hole, the sample to be detected moves forwardly by virtue of the effect of a capillary tube, after the colloid gold marker on the combination pad is dissolved, the sample to be detected has inter-reaction and then is moved to an area for fixing an antigen or antibody, the sample to be detected is specifically combined with the colloid gold marker to be intercepted and accumulated onto a detection strip, and a developing result can be observed by eyes. The test paper is simple and rapid in detection, free from complicated operation skills and special devices and convenient to carry; and in addition, the test paper can realize semi-quantitative analysis for theprogesterone, is sensitive and accurate in result, and is more objective and effective for evaluating the estrous situation of the female dog.

The invention belongs to the technical field of medical treatment, and particularly discloses an intrahepatic portosystem shunt stent with the diameter capable of being automatically adjusted. The intrahepatic portosystem shunt stent with the diameter capable of being automatically adjusted comprises an intrahepatic membrane segment, a non-projection line gold marker line, a portal bare segment and a through hole groove, wherein a right side end of the intrahepatic membrane segment is fixedly connected with the non-projection line gold marker line, a right side end of the non-projection line gold marker line is fixedly connected with the portal bare segment, a dilation head is fixedly installed at a right side end of the portal bare segment, multiple overhead hooks are evenly and fixedly installed on an outer wall of the dilation head, an inner membrane dilation opening is fixedly formed in an inner wall of the dilation head, and the through hole groove is formed in both inner walls ofthe intrahepatic membrane segment and the portal bare segment. The intrahepatic portosystem shunt stent with the diameter capable of being automatically adjusted has the comprehensive effects that the intrahepatic portosystem shunt stent with the diameter capable of being automatically adjusted can be effectively and steadily connected with a blood vessel, and is not liable to fall off, a successrate of installation is high, and meanwhile, the insertion depth can be observed conveniently.

The invention discloses antibiotics detection test paper. The antibiotics detection test paper comprises a sample absorption pad, a gold marker combination pad, a reaction film and absorption paper, wherein the gold marker combination pad is coated with a beta-lactam specific antibody-colloidal gold marker, a tetracycline specific antibody-colloidal gold marker, a quinolone specific antibody-colloidal gold marker, a macrolide specific antibody-colloidal gold marker, a sulfanilamide specific antibody-colloidal gold marker and a cephalosporin specific antibody-colloidal marker; the reaction film is coated with a macrolide antibiotics detection line, a beta-lactam antibiotics detection line, a tetracycline antibiotics detection line, a quinolone antibiotics detection line, a sulfanilamide antibiotics detection line, a cephalosporin antibiotics detection line and a goat-anti-mouse quality control line. The antibiotics detection test paper can realize combined detection of various antibiotics in urine of children, can be conveniently and quickly used without professional operation, and is suitable for household use.

The invention relates to a binary detection test strip of beta-exhilarant ractopamine and salbutamol and a preparation method thereof, which can effectively solve the problem that the beta-exhilarant ractopamine and salbutamol can not be detected quickly, easily and simultaneously. The preparation method comprises the following steps of: arranging diversion glass fiber, carrier glass fiber, a nitrocellulose membrane and an absorbent cotton pulp board a on a substrate PVC (Polyvinyl Chloride) board from back to front in order; covering a coating comprising a front coating and a rear coating onthe diversion glass fiber, the carrier glass fiber and the absorbent cotton pulp board, wherein a monoclonal antibody for resisting ractopamine protein conjugates and a monoclonal antibody colloid gold marker for resisting salbutamol protein conjugates are adsorbed in the carrier glass fiber; and coating the nitrocellulose membrane with a quality control line and two test lines, wherein the quality control line is coated with rabbit antimouse multi-resistant, and the test lines are coated with ractopamine protein conjugates and salbutamol protein conjugates. The invention can be used for detecting the beta-exhilarant ractopamine and the salbutamol simultaneously, effectively, conveniently and quickly and has accurate result.

The invention provides a method for removing residual gold at photolithographic marking points of LED chips, and relates to the technical field of LED chip production. The method includes: redesigning the lithography marking points of the LED chip; setting the lithography-related parameters of the lithography marking points of the LED chip after the redesign; setting the lithography marking points of the LED chip according to the parameters for evaporation temperature, and then vapor-deposit the photolithographic mark points of the LED chip on the wafer; after the vapor deposition of the photolithographic mark points of the LED chip is completed, the metal stripping process conditions are set, and the metal of the closed-loop disconnected part is stripped; Glue removal is performed on the closed-loop disconnected part on the photolithographic marking point of the LED chip after the metal is peeled off. The invention solves the problem that photolithography cannot be carried out after gold plating of the LED chip due to residual metal, thereby reducing the rework rate of preparation, ensuring product consistency, removing unstable factors, and improving product yield.

The invention discloses a test paper card for detecting aflatoxin B1 and application of the test paper card. The test paper card comprises a sample absorption pad, a conjugate release pad, a reactive film, a water absorption pad and a bottom plate, wherein a detection line coated with an aflatoxin B1 hapten-carrier protein conjugate and a quality control line coated with a goat anti-rat antibody are arranged on the reactive film; an aflatoxin B1 monoclonal antibody-colloid gold marker is sprayed on the conjugate release pad. The invention also provides a method for detecting aflatoxin B1 residues in grains and feed by using the aflatoxin B1 test paper card. The test paper card provided according to the invention has the characteristics of simplicity in operation, high sensitivity, high detection speed, low cost and the like and is suitable for screening and field monitoring of lots of samples.

The invention provides an immune colloidal gold test paper card for testing spiramycin. The test paper card comprises a reaction membrane, a sample pad, a gold marker pad, a water absorption pad and aPVC sheet, the reaction membrane has a testing area coated with a spiramycin hapten-carrier protein conjugate and a quality control area coated with goat anti mouse IgG, a conjugate releasing pad iscoated with a monoclonal spiramycin antibody-colloidal gold marker. The invention further provides a method utilizing the above-mentioned test paper card for testing spiramycin. The method comprises the steps that firstly, a sample is pretreated, then the pretreated sample is tested by the test paper card, and finally, the testing result is analyzed. The test paper card can be used for testing theamount of residual spiramycin medicine in animal-derived food such as chicken and feed, and is simple in operation, high in sensitivity, high in testing speed, low in cost, and capable of achieving on-site supervision and meeting the requirements for screening a large number of samples.

A removable dual gold marker implantation device with minor damage comprises a gold marker and a puncture needle, the gold marker is arranged in the needle barrel of the puncture needle when in use; the gold marker comprises a gold marker head and a connecting wire connected with the gold marker head; the gold marker head comprises a first gold marker, a connecting part and a second gold marker connected in sequence. The disclosure reduces the damage to the body when removing the gold marker, and the dual gold marker structure makes the implantation of multiple gold markers at the same time possible and reduces the number of times of implantation and improves the efficiency.

The invention discloses a detection method for 1-aminohydantoin hydrochloride in animal tissues and a detection card. The detection card and a reagent are provided, wherein the surface of the detection card is provided with a colloidal gold test strip; the inner side of an ELIASA hole is provided with a monoclonal colloid gold marker antibody of frozen 1-aminohydantoin hydrochloride; the top of the surface of the detection card is provided with comparison color blocks at equal intervals; the reagent comprises deionized water, 1M hydrochloric acid, a derivatization reagent, an extraction agent, 1M sodium hydroxide, gold chloride, double distilled water, a chloroauric acid solution, a 1-percent sodium citrate aqueous solution, 0.1mol / L K2CO2 and a 0.1mol / L HCL cloning solution. In the detection method, the monoclonal colloid gold marker antibody is used, so that covalent bonds between gold particles and antibody molecules are avoided in the monoclonal colloid gold marker antibody, and the metal particles and the antibody molecules are combined through Van der Waals force among charges of different polarities; the colloid gold marker has very small influences on the specificity and affinity of the antibody, so that the detection accuracy of the 1-aminohydantoin hydrochloride can be increased.

This invention discloses a way for fast checking the immunological colloid gold test strip left by the sulfadiazine and it's making method, which belongs to the immunochemistry fast measure technique filed. The test strip of this invention includes: the sample mat, the combining mat, the cellulose nitrate film, the sopping mat and the PVC backing, which characterized in that the sample mat, the combining mat, the cellulose nitrate film and the sopping mat inhibit the PVC backing in order; the combining mat is marked with the anti-sulfadiazine monoclonal antibody-colloid gold marker, and the monoclonal antibody is obtained by the secretion of the hybridomas cell line BDRXN(the conserving number is CCTCC-C200522) The cellulose nitrate film is covered with the checking line that is composed of the sulfadiazine-carrier protein joined matter and the quality control line that is composed of the rabbit-anti-mouse IgG. The test strip of this invention has the following advantages: the sensitivity is high, the special isomerism is strong, the operation is simple and checking is fast and exactly.

The invention provides an immune colloidal gold test paper card for testing diazepam. The test paper card comprises a reaction membrane, a sample pad, a gold marker pad, a water adsorption pad and a back sheet, the reaction membrane has a test area coated with a diazepam hapten-carrier protein conjugate and a quality control area coated with goat anti mouse IgG, and a conjugate releasing pad is coated with a diazepam monoclonal antibody-colloidal gold marker. The invention further provides a method utilizing the test paper card for testing diazepam. The method comprises the steps that firstly,a sample is pretreated, then the pretreated sample is tested by the test paper card, and finally, the test result is analyzed. The test paper card can be used for testing the amount of residual diazepam medicine in animal-derived food and urine samples, and is simple in operation, high in sensitivity, high in testing speed, low in cost, and capable of achieving on-site supervision and meeting therequirements for screening a large number of samples.

A method for detecting total IgG combined with Fc fragment of Staphylococci protein A (SPA) in mammalian serum with colloidal gold-labeled protein A comprises the following steps of: 1) construction of a standard curve and calculation of regression equation for each batch of colloidal gold-labeled probe and micro-reaction by (1) setting 8 standard holes, adding 50MuL 0.01M TBS solution (pH 7.4, containing 0.1% calf serum) to each hole; (2) adding 50MuL standard IgG solution from the first hole to the sixth hole by doubling dilution, sucking out 50MuL TBS solution from the seventh hole, adding50MuL standard IgG solution, and leaving the eighth hole as a blank control; (3) adding 50MuL colloidal gold-labeled probe diluted by 2-6 times in the reaction holes; (4) completely mixing the reaction plate, and reacting at room temperature for 20-40min; (5) detecting absorbance at 620nm with a microplate-reader for ELISA; and (6) calculating common logarithm of the absorbance associated with the standard IgG; and 2) sample detection by (1) adding 50MuL sample solution in the sample holes; (2) adding 50MuL diluted colloidal gold-labeled probe in the sample holes; (3) completely mixing the reaction plate, and reacting at room temperature for 20-40min; and (4) detecting absorbance of each hole at 620nm with the microplate-reader for ELISA, and calculating corresponding IgG values according to the regression equation of the standard curve.

The invention relates to an extracorporal test kit for TAFI content, and a test method of the extracorporal test kit. The extracorporal test kit for the TAFI content consists of a reagent, a TAFI standard product and a TAFI quality control product, wherein the reagent contains a monoclonal antibody nano-gold marker R that is specifically bonded with TAFI; the TAFI standard product and TAFI quality control product are used for making a standard curve for calculating the content of a TAFI antigen in a to-be-tested sample. The biological characteristic that nano-gold is bonded with an antibody, and a conventional biochemical test method is combined, so that the extracorporal test method of the extracorporal test kit is used for an extracorporal test of the content of the TAFI, a new extracorporal diagnostic method is developed for the TAFI, and the sensitivity of the extracorporal test of the TAFI is improved. According to the extracorporal test method, the problem that the equipment is expensive in the prior art is solved, the detectable rate and the accuracy rate on relevant diseases are improved, unnecessary pains and medical cost of a tested person are reduced, and the living quality of the tested person is improved.