39 results about "Test organism" patented technology

Presented herein are systems and methods for classifying biomedical images for executing operations. A computing system may identify a biomedical image of a slide with a biological sample obtained from a subject at risk of a condition; apply the biomedical image to a machine learning model; generate, based on applying the biomedical image to the ML model, a classification corresponding to the biomarker associated with the condition in biological sample on the slide; and execute an operation with respect to the slide for testing of the biological sample, in accordance with the classification for the biomedical image.

Provided is a system (100) for use in testing biological material. The system includes a substrate (102), and at least one support member (104) that has a biocompatible support portion (106) for supporting the biological material. The at least one support member is mountable to, and detachable from, the substrate. The at least one support member is detachable from the substrate at least by manual manipulation by a user, for example by the user pulling the at least one support member from the substrate using tweezers. Additionally provided is the support member per se. Further provided is a method of testing biological material using the system, as well as use of such a system for testing biological material.

The utility model relates to the technical field of circulating water quality detection devices, and particularly discloses an industrial circulating water simulation device for testing the scale and corrosion inhibition performance of a biological agent. The heating assembly can heat the circulating cooling water in the first water tank; a to-be-detected pipeline quick connector is sleeved in the water supply pipe; the spraying assembly is communicated with one end of the water supply pipe fitting and is used for spraying circulating cooling water into the first water tank; a water inlet of the delivery pump is communicated with the first water tank, and a water outlet of the delivery pump is communicated with one end, away from the spraying assembly, of the water supply pipe; the hanging piece assembly is provided with a plurality of metal pieces, and the metal pieces are located below the water outlet of the spraying assembly; and the biological membrane frame is filled with a bacteria-containing carrier, is immersed in the first water tank, and can simulate different production environments and test the scale and corrosion inhibition performance of the biological agent.

The present invention provides a test method for toxicity of raw dinotefuran drug on early life stage of fish. The method includes: preparing a standard diluent, preparing test drug liquids, selecting a test organism, toxicant exposure of the embryos, toxicant exposure of larvae, monitoring and controlling test conditions, observation in the test, and statistically analyzing data and drawing results.

Oxadiazole antibiotics that exhibit bactericidal activity against C. difficile vegetative cells. We screened a library of 75 oxadiazoles against C. difficile ATCC 43255. The findings from this collection served as the basis for the syntheses of an additional 58 analogs, which were tested against the same strain. We discovered a potent (MIC50=0.5 μg / mL, MIC90=1 μg / mL for 101 C. difficile strains) and narrow-spectrum oxadiazole (3-(4-(cyclopentyloxy)phenyl)-5-(4-nitro-1H-imidazol-2-yl)-1,2,4-oxadiazole; compound 57) that is not active against common gut bacteria or other tested organisms, but is selectively bactericidal against C. difficile and targets cell-wall synthesis. Other similarly effective oxadiazole antibiotics of formula I and II are described herein, several of which inhibit or prevent C. difficile spore germination.

The invention provides a pond microcosm method for evaluating pollutant influence. The method comprises the following steps: S1, constructing a pond microcosm system for dynamic analogue simulation; s2, putting the tested organism into the pond microcosm system, exposing the to-be-evaluated pollutants in the pond microcosm system, and after a period of time, analyzing to obtain the enrichment concentration of the to-be-evaluated pollutants in the water phase, the sediment and the tested organism; s3, analyzing and calculating the data to obtain the fugacity of the environmental phase and the fugacity capacity of the environmental phase; and S4, calculating a net change flux through a fugacity model, and evaluating the dynamic change of the pollutants in the pond microcosm system. According to the method, the experimental method is improved and the fugacity model is introduced, so that the defects of complexity, authenticity and analysis in the microcosm experiment in the prior art are effectively overcome, and a more perfect and accurate method is provided for deeply researching environmental behaviors of pollutants in a microcosm ecological system in a near-natural state.

The application discloses a kind of composite pollutant comprehensive toxicity effect determination method, comprising: constructing comprehensive toxicity model, obtaining the multidimensional behavior toxicity index for characterizing the change of test organism exposed to pollutants, construct comprehensive toxicity prediction model based on deep learning;With the multidimensional behavior toxicity index of the sewage to be tested as input, the comprehensive toxicity model is used to predict, and the comprehensive toxicity index prediction value is obtained, to determine whether the sewage to be tested exists toxicity effect.Compared with the traditional toxicity evaluation with death endpoint as criterion, the application is aimed at composite pollutants in sewage and actual sewage samples, while ensuring that the multidimensional behavior toxicity information of daphnia magna is fully characterized, the normalized expression of comprehensive toxicity index is realized, and the toxicity effect determination method based on ATI is established, which can be widely used in the comprehensive toxicity effect determination of composite pollutants.

The invention belongs to the field of experimental devices, and discloses a flexible heat insulation soft sheet type small water body temperature gradient experimental device and an experimental method.The experimental device comprises a water tank, the water tank has the length direction, the water tank extends in the length direction, an in-tank cavity is formed in the water tank, and the in-tank cavity is matched with the water tank; a heating unit and a refrigerating unit are arranged outside the two ends of the water tank respectively, and the heating unit and the refrigerating unit are started at the same time so as to heat and refrigerate water in the inner cavity of the water tank at the same time; a plurality of flexible heat insulation soft pieces are arranged in the in-groove cavity, the flexible heat insulation soft pieces are arranged in the in-groove cavity at equal intervals in the length direction, and a gap exists between the lower surface of each flexible heat insulation soft piece and the bottom of the in-groove cavity. The device is suitable for a small-size water body environment, the heating unit and the refrigerating unit provide a stable heating end and a stable cooling end at the same time, water temperature gradient and temperature constant boundary conditions are formed, hot water and cold water convection is achieved through the flexible heat insulation film and the gap, and the gap also gives consideration to water body communication and tested organism passing.

This invention relates to a low-dose aggregation-induced emission dye (AIE) bioimaging method, comprising the following steps: selecting a test organism and injecting it with a low dose of AIE; stimulating the test organism with an adjustable frequency excitation light source; acquiring fluorescence signals emitted from the test organism at equal time intervals; processing the fluorescence signals and generating a fluorescence image; applying phase-locked processing to the fluorescence image to separate the fluorescence signal component with the same frequency as the excitation light source and generating a phase-locked imaging result. By convolving the amplitude signal of each pixel in the fluorescence image with a sine or cosine signal with the same frequency as the excitation source, the orthogonality of trigonometric functions is utilized to effectively suppress noise, thus preserving the fluorescence signal that changes with the same frequency as the periodic light source. This significantly improves the signal-to-noise ratio of the fluorescence image, enabling clear observation of the AIE fluorescence signal even at low doses.

The utility model discloses a tracheal cannula comprehensive performance testing arrangement, including test organism, the inside embedded fixed water bath kettle body of test organism, the top of water bath kettle body is provided with the pot cover, and the inside of water bath kettle body is provided with the support mechanism, and the support mechanism includes fixed bolster and catheter positioning frame, and the one end of fixed bolster upper surface is rotatably connected in catheter positioning frame, and the one side surface of catheter positioning frame is arranged and fixed with catheter positioning clamp, and the below of pot cover is provided with elevating system, and elevating system includes elevating slide sleeve, and the inside sliding connection of elevating slide sleeve has elevating slide bar, the utility model discloses the design of catheter positioning frame support pot cover makes pot cover not to need to take down completely to realize the state of suspension of " to use " through the standing of catheter positioning frame. This design completely solved the problem that traditional separation type pot cover is difficult to place properly because of the compactness of test organism mesa space, and the operation process of pot cover taking is simplified.

The invention relates to a method (500) for testing a sample for specified sample parameters using a microfluidic system (1000), in particular for detecting pathogens, wherein the microfluidic system (1000) is designed to test a biological sample for a set of various sample parameters, wherein one or more sample parameters are selected from the set, and the sample is tested for the selected sample parameters. The invention further relates to a microfluidic system (1000) for testing a sample for specified sample parameters, the microfluidic system being designed to carry out a method (500) according to the invention.

The present application belongs to the technical field of biological toxicity test of chemicals, and particularly relates to a chemical toxicity test method based on a self-developed pollutant exposure system. The test method is used for simulating the toxicity of chemicals in natural water bodies by means of a self-developed pollutant exposure system, adopts zebrafish as a model organism, establishes a time-concentration linear function of the tested chemicals in a poisoning area, and calculates the toxicity test method of the minimum effect concentration of the tested chemicals by observing the abnormal behavior of zebrafish or analyzing the behavior by coupling a camera tracking technology. The test method is almost a non-destructive experiment, the experimental time is short, most of the zebrafish participating in the experiment can be restored to normal state through appropriate recovery, the time cost is greatly saved, and the test is efficient and has a low mortality rate of test organisms.

Synthetic genetic platforms in eukaryote cells (such as yeast) is described. A representative synthetic genetic platform includes a eukaryote cell genetically modified to express an auxin receptor, an auxin response factor, and a reporter, as well as a fusion construct including a Lis1 Homology (LisH) domain fused to an auxin-responsive protein. The synthetic genetic platforms can be used, for instance, to understand developmental and pathological LisH domain variants, and to test bioactive molecules for LisH domain activity.

The application discloses a method for efficiently detecting the distribution and abundance of DNA binding proteins at the whole genome level of fungi. The application provides a method for detecting the distribution and abundance of target proteins in the whole genome of a test organism, characterized in that the organism is fungi, and the target protein is a DNA binding protein in the test fungi; the method for detecting the distribution and abundance of target proteins in the whole genome of a test organism provided by the application is fungal CUT&Tag; compared with traditional CUT&Tag, the difference of the fungal CUT&Tag lies in that the following step is added before adsorbing the cell nucleus: taking fungal hyphae, performing formaldehyde cross-linking, and then preparing protoplasts; the following step is added between fragmentation and library amplification: removing formaldehyde cross-linking. The application first realizes the application of the CUT&Tag technology to the detection of the distribution and abundance of DNA binding proteins of filamentous fungi at the genome level, and provides an important means for the research on fungal DNA binding proteins.

The invention belongs to the technical field of biotoxicity testing, and discloses branched chain fatty acid and application thereof in inhibiting and killing caenorhabditis elegans. Model organism caenorhabditis elegans are adopted as a tested organism, 3-methylvaleric acid is adopted as an exogenous medicine, an experimental group and a control group with different concentration gradients are set, the death rates of the caenorhabditis elegans in 24 hours, 48 hours and 72 hours are calculated, and the death rate of the caenorhabditis elegans in the experimental group is calculated. And an LC50 value is calculated. Apparent structure analysis and transcriptome test analysis are carried out by means of an electron microscope, fluorescent staining and the like, and the toxic action mechanism of exogenous drugs on nematodes is explored. The research systematically reveals the toxicity mechanism of 3-methylvaleric acid for the first time, and provides a theoretical basis for the future research and development of 3-methylvaleric acid.

1. Name of the product in this design: Chemical Detection and Analysis Instrument. 2. Purpose of this design: For testing chemical substances in biological samples. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The present disclosure relates to nucleic acid amplification devices for testing a bodily fluid sample for a biological analyte and their methods of use. Disclosed herein are nucleic acid amplification systems for untrained users or lay persons. The nucleic acid amplification devices have, e.g., a housing with valves, one or more incubation and detection chambers configured to enable the amplification and the detection of nucleic acids.

Summarizing the invention, a method is provided. The method comprises: accessing, by a computing device, test order data, wherein the test order data comprise information specifying a set of clinical tests to be carried out by a laboratory instrument on a biological sample contained in a sample container, wherein each clinical test is associated with a set of retain conditions so that the sample container is associated with one or more sets of retain conditions; determining, by the computing device, whether at least one set of retain conditions associated with the sample container is met; and if at least one set of retain conditions associated with the sample container is met, causing, by the computing device, the sample container to be retained in the laboratory instrument; wherein each set of retain conditions associated with a respective clinical test comprises a condition that one or more rules require that one or more supplementary tests shall be carried out whenever one or more supplementary test conditions on the respective clinical test are met.

Methods and devices for testing a biological sample are provided. A tape includes multiple channels or reservoirs having inlet and outlet ports. One tape having biological sample disposed in its channels is temporarily mated with another tape having reagents disposed in its channels via a serpentine belt and compression roller assembly. Pulsed fluidic operations combine the reagents and the biological sample for subsequent observation, detection, storage and / or disposal. Fluidic transfer is provided in a uniform operation or in conjunction with a sensory feedback assembly.

The invention provides an ampullaria gigas-based prochloraz pesticide ecotoxicity detection method, which comprises the following steps: collecting or domesticating ampullaria gigas, and distinguishing individuals in a new hatching period and individuals in an adult period as test organisms; the method comprises the following steps: respectively exposing new hatching period pomacea canaliculata and adult period pomacea canaliculata in prochloraz aqueous solutions with different concentrations, exposing for 96 hours, and regularly observing and recording the survival individual number, the inhibited individual number and the individual number with escape behavior in each group during the period; the method comprises the following steps: exposing adult pomacea canaliculata in a series of prochloraz aqueous solutions with sublethal concentrations for 21 days, and regularly measuring and recording shell height and weight of the pomacea canaliculata bodies during exposure; taking the exposed hepatopancreas, kidney and stomach tissues of the ampullaria gigas, and carrying out histopathologic observation and analysis; the invention also provides application of the detection method in evaluating the ecotoxicity of the prochloraz pesticide. The pesticide toxicity can be rapidly and systematically tested through the easily obtained pomacea canaliculata, and the detection method is comprehensive and convenient to operate.

A method for high-throughput determination of whole water toxicity, including: exposing test organisms in a wastewater sample for pollution, obtaining phenotypic feature data of the test organisms; constructing a toxicity matrix; and building a machine learning model, and in combination with the toxicity matrix, determining a whole toxicity of the wastewater sample.

The invention relates to the field of biotoxicity detection, in particular to a biotoxicity evaluation method and device based on heartbeat signals of daphnia organisms and a storage medium. The method comprises the following steps: acquiring heartbeat signal characteristics of daphnia organisms under the action of a to-be-detected water sample, and according to response characteristics of the heartbeat signal characteristics in the to-be-detected water sample containing different pollutants, outputting by adopting a pre-trained biotoxicity prediction model to obtain a biotoxicity evaluation result, the biotoxicity evaluation result is used for indicating the biotoxicity of the water sample to be detected. According to the embodiment of the invention, the heartbeat signal characteristics of the daphnia organisms under the action of the water sample to be detected are obtained, and the biotoxicity evaluation result is quickly output by utilizing the biotoxicity prediction model, so that the problems of low sensitivity, long detection period, complicated operation, invasive injury to the tested organisms caused by invasive detection and the like in the traditional method are solved; the rapid and high-sensitivity toxicity assessment of water pollutants is realized; and the daphnia tested organisms are noninvasive and nondestructive in the whole process.

A lateral flow assay (LFA) device (10) for testing the presence of a target analyte in a biofluid sample comprises a longitudinal backing (12) having a hybrid sample / conjugate pad (18), a testing membrane (26) downstream the hybrid sample / conjugate pad (18) and an absorbent pad (34) downstream the testing membrane (26) extending thereon. The hybrid sample / conjugate defines a first treated section (18a) comprising a protein precipitating agent (20), a second treated section (18b) comprising a target analyte specific conjugate agent (22), and a third treated section (18c) comprising a flow assisting agent (24). The biofluid sample is collected by the hybrid sample / conjugate pad which provides for laminar flow of the biofluid sample through the first, second and third treated sections in sequence. The test membrane comprises detection lines (28) comprising respective detection agent for detecting the presence of the target analyte in the biofluid sample flowing thereon from the hybrid sample / conjugate pad (18) and into the absorption pad (34).

The present disclosure describes systems and methods for estimating the immobilization status of a test biological sample or any portion thereof treated with one or more immobilizing agents for morphological staining of an unknown duration. For example, the systems and methods of the present disclosure may estimate that the immobilization state of a morphologically stained test biological sample is one of "insufficient immobilization", "immobilized", or "over-immobilized".

The present invention discloses a method for quantitatively assessing the offshore ecological risk of modified clay. The method comprises culturing three test organisms; conducting exposure experiments based on the field use concentration of the modified clay; detecting biomarkers of each species; calculating the IBR value of each species using an improved integrated biomarker response (IBR) evaluation method; summing the IBR values ​​to obtain a total IBR value; statistically analyzing the total IBR value to obtain a significance P value; and using the total IBR value and the P value to jointly characterize the ecological risk level. Based on a multi-species biomarker indicator system and by improving the value assignment and calculation methods of the IBR evaluation method, the present invention establishes a quantitative assessment method for offshore ecological risk of modified clay.