74 results about "Test organism" patented technology

The invention discloses a water quality comprehensive toxicity high-throughput determination method, which comprises the following steps: performing exposure contamination on a tested organism by using a sewage sample to obtain phenotypic characteristic data of the tested organism; constructing a toxicity matrix; establishing a machine learning model, and determining the water quality comprehensive toxicity of the sewage sample in combination with the toxicity matrix. The water quality comprehensive toxicity determination method provided by the invention does not need sample concentration and enrichment, ensures the comprehensiveness and accuracy of the detection result, and can be applied to the comprehensive toxicity high-flux determination of the sewage in the whole process of a sewage treatment plant.

The invention discloses a method and a sensor for testing the concentration of an analyte in a biological sample based on a multi-excitation mode, and belongs to the technical field of instant detection of the concentration of the analyte in the biological sample. The method comprises the following steps: inputting a first excitation signal and a second excitation signal to a first working electrode; inputting an excitation signal sequence to the second working electrode between the first excitation signal and the second excitation signal; acquiring the first excitation signal, the second excitation signal and the output current of the current acquisition point of the excitation signal sequence at regular intervals to obtain an output current group; and acquiring output voltages of voltage acquisition points of the first working electrode attenuation signal, the second working electrode attenuation signal and the counter electrode attenuation signal at regular intervals to obtain an output voltage group. According to the method, the mutual influence relationship among various interference factors related to the analyte is eliminated or weakened, and the accuracy, precision and aging resistance of concentration detection of the analyte in the biological sample are improved.

The invention discloses a method for determining a micro-plastic particle seawater ecological risk evaluation threshold value. The method comprises the following steps: (1) preparing pure micro-plastic; (2) carrying out biological testing; and (3) deducing the threshold value. According to the method, the toxic effect of pure micro-plastic can be measured, instead of the composite toxic effect of the micro-plastic and the leaching additive, and the toxicity of the micro-plastic particles to marine organisms can be evaluated more accurately; in addition, the tested organisms adopted by the method are marine organisms, so that the risk of the micro-plastic particles to seawater ecology can be reflected more accurately; the method can also derive the toxicity threshold value of the micro-plastic particles to marine organisms.

Presented herein are systems and methods for classifying biomedical images for executing operations. A computing system may identify a biomedical image of a slide with a biological sample obtained from a subject at risk of a condition; apply the biomedical image to a machine learning model; generate, based on applying the biomedical image to the ML model, a classification corresponding to the biomarker associated with the condition in biological sample on the slide; and execute an operation with respect to the slide for testing of the biological sample, in accordance with the classification for the biomedical image.

To provide model-based featurization and classifiers for predicting a disease state from nucleic acid samples.SOLUTION: In various embodiments, an analytics system uses models to determine features and classification of a disease state. The disease state can indicate presence or absence of cancer, a cancer type, or a cancer tissue of origin. The models can include a binary classifier and a tissue of origin classifier. The analytics system can process sequence reads from test biological samples to generate data for training the classifiers. The analytics system can also use combinations of machine learning techniques to train the models, which can include a multilayer perceptron. In some embodiments, the analytics system uses methylation information to train the models to determine predictions regarding the disease state.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of chemical biotoxicity testing, and particularly relates to a chemical toxicity testing method based on a self-developed pollutant exposure system. According to the testing method, a self-developed pollutant exposure system is used for simulating the toxic effect of chemicals in a natural water body, zebra fish is adopted as a model organism, a time-concentration linear function of to-be-tested chemicals in a contaminated area is established, behavior analysis is carried out through observation of abnormal behaviors of the zebra fish or a coupled camera tracing technology, and the method is used for determining the toxic effect of the to-be-tested chemicals in the contaminated area. And calculating the minimum effect concentration of the to-be-tested chemical. The test method is almost a non-destructive experiment, the experiment time is short, most zebrafish participating in the experiment can be recovered to a normal state through proper recovery, the time cost is greatly saved, the efficiency is high, and the death rate of tested organisms is low.

The present invention relates to methods of treating animals with an antimicrobial growth promoter (AGP) to promote a healthy microbiome, the method comprising: a) providing test organisms and a control organism, b) administering a regular regimen of a different AGP to each test organism for about two months, c) collecting DNA from the intestinal content of the test organisms and of the control organism, d) sequencing the DNA of the intestinal contents of the organisms to obtain taxonomic and functional profiles, thereby defining core intestinal microbiomes of the test and control organisms, e) comparing the core intestinal microbiomes of the test organisms and of the control organism, thereby determining which test organism has a healthy microbiome thereby determining which AGP provides the most healthy microbiome, and f) treating the domesticated organism with the AGP which provides the healthy microbiome.

In various embodiments, an analysis system uses a model to determine features and classifications of a disease state. The disease state may indicate the presence or absence of cancer, the type of cancer, or the tissue of origin of cancer. The model may include a binary classifier and a source organization classifier. The analysis system may process sequence reads from a test biological sample to produce data for training the classifier. The analysis system may also train the model using a combination of machine learning techniques, which may include a multi-layer perceptron. In some embodiments, the analysis system uses methylation information to train the model to determine a prediction regarding a disease state.

Provided is a system (100) for use in testing biological material. The system includes a substrate (102), and at least one support member (104) that has a biocompatible support portion (106) for supporting the biological material. The at least one support member is mountable to, and detachable from, the substrate. The at least one support member is detachable from the substrate at least by manual manipulation by a user, for example by the user pulling the at least one support member from the substrate using tweezers. Additionally provided is the support member per se. Further provided is a method of testing biological material using the system, as well as use of such a system for testing biological material.

The utility model relates to the technical field of circulating water quality detection devices, and particularly discloses an industrial circulating water simulation device for testing the scale and corrosion inhibition performance of a biological agent. The heating assembly can heat the circulating cooling water in the first water tank; a to-be-detected pipeline quick connector is sleeved in the water supply pipe; the spraying assembly is communicated with one end of the water supply pipe fitting and is used for spraying circulating cooling water into the first water tank; a water inlet of the delivery pump is communicated with the first water tank, and a water outlet of the delivery pump is communicated with one end, away from the spraying assembly, of the water supply pipe; the hanging piece assembly is provided with a plurality of metal pieces, and the metal pieces are located below the water outlet of the spraying assembly; and the biological membrane frame is filled with a bacteria-containing carrier, is immersed in the first water tank, and can simulate different production environments and test the scale and corrosion inhibition performance of the biological agent.

The present invention provides a test method for toxicity of raw dinotefuran drug on early life stage of fish. The method includes: preparing a standard diluent, preparing test drug liquids, selecting a test organism, toxicant exposure of the embryos, toxicant exposure of larvae, monitoring and controlling test conditions, observation in the test, and statistically analyzing data and drawing results.

The utility model belongs to the technical field of soil biological comprehensive toxicity detection with soil habitat functions, and discloses a volatile organic contaminated soil biological toxicity testing device which comprises a soil sample bottle filled with soil and a gas washing and testing device bottle filled with a gas washing solution. The gas washing and testing device further comprises a buffering mechanism which is arranged on the first gas guide pipe, located on the inner side wall of the gas washing and testing device bottle and used for buffering impact force of backflow gas, the buffering mechanism comprises a mounting box fixedly connected to the side wall of the first gas guide pipe, and a buffering ring is connected with a buffering plate through a plurality of extrusion assemblies. According to the volatile organic contaminated soil biotoxicity testing device, through the arrangement of the buffer mechanism, backflow gas is buffered, the impact force on a gas washing solution is reduced, and therefore the generation probability of bubbles is reduced, and the testing efficiency is improved. The reduction of the content of volatile organic compounds in the soil and the influence on the physiological state and behavior of a tested organism caused by excessive bubbles are avoided.

Oxadiazole antibiotics that exhibit bactericidal activity against C. difficile vegetative cells. We screened a library of 75 oxadiazoles against C. difficile ATCC 43255. The findings from this collection served as the basis for the syntheses of an additional 58 analogs, which were tested against the same strain. We discovered a potent (MIC50=0.5 μg / mL, MIC90=1 μg / mL for 101 C. difficile strains) and narrow-spectrum oxadiazole (3-(4-(cyclopentyloxy)phenyl)-5-(4-nitro-1H-imidazol-2-yl)-1,2,4-oxadiazole; compound 57) that is not active against common gut bacteria or other tested organisms, but is selectively bactericidal against C. difficile and targets cell-wall synthesis. Other similarly effective oxadiazole antibiotics of formula I and II are described herein, several of which inhibit or prevent C. difficile spore germination.

The invention provides a pond microcosm method for evaluating pollutant influence. The method comprises the following steps: S1, constructing a pond microcosm system for dynamic analogue simulation; s2, putting the tested organism into the pond microcosm system, exposing the to-be-evaluated pollutants in the pond microcosm system, and after a period of time, analyzing to obtain the enrichment concentration of the to-be-evaluated pollutants in the water phase, the sediment and the tested organism; s3, analyzing and calculating the data to obtain the fugacity of the environmental phase and the fugacity capacity of the environmental phase; and S4, calculating a net change flux through a fugacity model, and evaluating the dynamic change of the pollutants in the pond microcosm system. According to the method, the experimental method is improved and the fugacity model is introduced, so that the defects of complexity, authenticity and analysis in the microcosm experiment in the prior art are effectively overcome, and a more perfect and accurate method is provided for deeply researching environmental behaviors of pollutants in a microcosm ecological system in a near-natural state.

The invention discloses a method for efficiently detecting distribution and abundance of fungal whole genome level DNA (Deoxyribose Nucleic Acid) binding protein. The invention provides a method for detecting the distribution and abundance of a target protein in a whole genome of a tested organism, which is characterized in that the organism is a fungus, and the target protein is a DNA (Deoxyribose Nucleic Acid) binding protein in the tested fungus; the method for detecting the distribution and abundance of the target protein in the whole genome of the tested organism provided by the invention is a fungus CUTamp; a Tag; the method is compared with a conventional CUTamp; in comparison with Tag, the fungi CUTamp; tag is characterized in that the following steps are added before cell nucleus adsorption: fungal hyphae are taken for formaldehyde crosslinking, and then protoplast is prepared; a step of removing formaldehyde crosslinking is added between fragmentation and library amplification. According to the method and the device, the CUAMP is firstly converted into the CUAMP; the Tag technology is applied to detection of distribution and abundance of the DNA binding protein of the filamentous fungi in the genome level, and an important means is provided for related research of the DNA binding protein of the fungi.

The application discloses a kind of composite pollutant comprehensive toxicity effect determination method, comprising: constructing comprehensive toxicity model, obtaining the multidimensional behavior toxicity index for characterizing the change of test organism exposed to pollutants, construct comprehensive toxicity prediction model based on deep learning;With the multidimensional behavior toxicity index of the sewage to be tested as input, the comprehensive toxicity model is used to predict, and the comprehensive toxicity index prediction value is obtained, to determine whether the sewage to be tested exists toxicity effect.Compared with the traditional toxicity evaluation with death endpoint as criterion, the application is aimed at composite pollutants in sewage and actual sewage samples, while ensuring that the multidimensional behavior toxicity information of daphnia magna is fully characterized, the normalized expression of comprehensive toxicity index is realized, and the toxicity effect determination method based on ATI is established, which can be widely used in the comprehensive toxicity effect determination of composite pollutants.

An example cartridge has an opening for a dispenser used to inject a sample fluid into the cartridge, a reservoir to receive the sample fluid, and a view window through which the sample fluid is viewable to a user of the cartridge. A member coupled to the view window at a predetermined position may indicate an amount of fluid suitable for a diagnostic test performed by the cartridge. The cartridge may include surfaces angled with respect to the each other to define a slope for flow of sample fluid down from the opening when the cartridge is upright. An instrument may include a physical interface to hold a cartridge during sample injection, and provide a user interface with prompts corresponding to injection of sample fluid and / or performance of the diagnostic test. A sensor may detect a state of the cartridge or its contents before, during, and / or after the diagnostic test.

The invention discloses a data processing device and system for detecting the aging degree and application of the data processing device and system. The technical problem to be solved is to detect the aging degree of an organism to be tested or cells to be tested. Specifically disclosed is a device, the device comprising: M1, a migration body condition acquisition module for acquiring the migration body condition of a subject body or a subject cell, the migration body condition comprising the number of migration bodies or the content of the migration bodies; the result output module is used for comparing the condition of the migration body of the tested organism or the tested cell with a reference value and giving a result, and the result comprises at least one of the following: C1, the aging degree of the organism or the cell; c2, physiological age of the organism or the cell, C3, whether the organism or the cell suffers from the senescence-related disease or not or the risk of the senescence-related disease, and C4, the senescence and / or age-related disease early-warning result can be judged through the device, and the senescence degree of the tested organism or the tested cell can be judged through the device.

The invention belongs to the field of experimental devices, and discloses a flexible heat insulation soft sheet type small water body temperature gradient experimental device and an experimental method.The experimental device comprises a water tank, the water tank has the length direction, the water tank extends in the length direction, an in-tank cavity is formed in the water tank, and the in-tank cavity is matched with the water tank; a heating unit and a refrigerating unit are arranged outside the two ends of the water tank respectively, and the heating unit and the refrigerating unit are started at the same time so as to heat and refrigerate water in the inner cavity of the water tank at the same time; a plurality of flexible heat insulation soft pieces are arranged in the in-groove cavity, the flexible heat insulation soft pieces are arranged in the in-groove cavity at equal intervals in the length direction, and a gap exists between the lower surface of each flexible heat insulation soft piece and the bottom of the in-groove cavity. The device is suitable for a small-size water body environment, the heating unit and the refrigerating unit provide a stable heating end and a stable cooling end at the same time, water temperature gradient and temperature constant boundary conditions are formed, hot water and cold water convection is achieved through the flexible heat insulation film and the gap, and the gap also gives consideration to water body communication and tested organism passing.

This invention relates to a low-dose aggregation-induced emission dye (AIE) bioimaging method, comprising the following steps: selecting a test organism and injecting it with a low dose of AIE; stimulating the test organism with an adjustable frequency excitation light source; acquiring fluorescence signals emitted from the test organism at equal time intervals; processing the fluorescence signals and generating a fluorescence image; applying phase-locked processing to the fluorescence image to separate the fluorescence signal component with the same frequency as the excitation light source and generating a phase-locked imaging result. By convolving the amplitude signal of each pixel in the fluorescence image with a sine or cosine signal with the same frequency as the excitation source, the orthogonality of trigonometric functions is utilized to effectively suppress noise, thus preserving the fluorescence signal that changes with the same frequency as the periodic light source. This significantly improves the signal-to-noise ratio of the fluorescence image, enabling clear observation of the AIE fluorescence signal even at low doses.

Technology for testing biologic substances deposited in a toilet is described. In one aspect, a device for testing biologic substances is located within the bowl of the toilet. The biologic testing device includes methods for testing the biologic substances within the bowl of the toilet, in a diversion pipe of the toilet, or in a reservoir connected to or located in a toilet. The biologic testing device includes colorimetric indicators to show the presence of specific compounds, or the concentrations of specific compounds. A scanning device for reading the colorimetric results is a part of the biologic testing system. The scanning device is connected to a computing device which interprets the colorimetric results and, in some embodiments, converts from a colorimetric reading to a text-based analysis which is displayed for a user to review.

The utility model discloses a tracheal cannula comprehensive performance testing arrangement, including test organism, the inside embedded fixed water bath kettle body of test organism, the top of water bath kettle body is provided with the pot cover, and the inside of water bath kettle body is provided with the support mechanism, and the support mechanism includes fixed bolster and catheter positioning frame, and the one end of fixed bolster upper surface is rotatably connected in catheter positioning frame, and the one side surface of catheter positioning frame is arranged and fixed with catheter positioning clamp, and the below of pot cover is provided with elevating system, and elevating system includes elevating slide sleeve, and the inside sliding connection of elevating slide sleeve has elevating slide bar, the utility model discloses the design of catheter positioning frame support pot cover makes pot cover not to need to take down completely to realize the state of suspension of " to use " through the standing of catheter positioning frame. This design completely solved the problem that traditional separation type pot cover is difficult to place properly because of the compactness of test organism mesa space, and the operation process of pot cover taking is simplified.

Methods for preparing a biological sample for testing by Maldi where such methods are selected based on sample parameters. Maldi scores are obtained for a range of sample parameters (e.g. McFarland, dispense volume and number of dispenses). From the data, sample preparation parameters can be selected for a biological sample being prepared for Maldi testing. One sample preparation strategy uses multiple dispenses of sample with an intervening drying step, which yields more accurate Maldi scores, particularly for samples at the low range of McFarland values (e.g. below about 2).

The invention relates to a method (500) for testing a sample for specified sample parameters using a microfluidic system (1000), in particular for detecting pathogens, wherein the microfluidic system (1000) is designed to test a biological sample for a set of various sample parameters, wherein one or more sample parameters are selected from the set, and the sample is tested for the selected sample parameters. The invention further relates to a microfluidic system (1000) for testing a sample for specified sample parameters, the microfluidic system being designed to carry out a method (500) according to the invention.

The present application belongs to the technical field of biological toxicity test of chemicals, and particularly relates to a chemical toxicity test method based on a self-developed pollutant exposure system. The test method is used for simulating the toxicity of chemicals in natural water bodies by means of a self-developed pollutant exposure system, adopts zebrafish as a model organism, establishes a time-concentration linear function of the tested chemicals in a poisoning area, and calculates the toxicity test method of the minimum effect concentration of the tested chemicals by observing the abnormal behavior of zebrafish or analyzing the behavior by coupling a camera tracking technology. The test method is almost a non-destructive experiment, the experimental time is short, most of the zebrafish participating in the experiment can be restored to normal state through appropriate recovery, the time cost is greatly saved, and the test is efficient and has a low mortality rate of test organisms.