28 results about "Test organism" patented technology

Presented herein are systems and methods for classifying biomedical images for executing operations. A computing system may identify a biomedical image of a slide with a biological sample obtained from a subject at risk of a condition; apply the biomedical image to a machine learning model; generate, based on applying the biomedical image to the ML model, a classification corresponding to the biomarker associated with the condition in biological sample on the slide; and execute an operation with respect to the slide for testing of the biological sample, in accordance with the classification for the biomedical image.

Provided is a system (100) for use in testing biological material. The system includes a substrate (102), and at least one support member (104) that has a biocompatible support portion (106) for supporting the biological material. The at least one support member is mountable to, and detachable from, the substrate. The at least one support member is detachable from the substrate at least by manual manipulation by a user, for example by the user pulling the at least one support member from the substrate using tweezers. Additionally provided is the support member per se. Further provided is a method of testing biological material using the system, as well as use of such a system for testing biological material.

Oxadiazole antibiotics that exhibit bactericidal activity against C. difficile vegetative cells. We screened a library of 75 oxadiazoles against C. difficile ATCC 43255. The findings from this collection served as the basis for the syntheses of an additional 58 analogs, which were tested against the same strain. We discovered a potent (MIC50=0.5 μg / mL, MIC90=1 μg / mL for 101 C. difficile strains) and narrow-spectrum oxadiazole (3-(4-(cyclopentyloxy)phenyl)-5-(4-nitro-1H-imidazol-2-yl)-1,2,4-oxadiazole; compound 57) that is not active against common gut bacteria or other tested organisms, but is selectively bactericidal against C. difficile and targets cell-wall synthesis. Other similarly effective oxadiazole antibiotics of formula I and II are described herein, several of which inhibit or prevent C. difficile spore germination.

The invention provides a pond microcosm method for evaluating pollutant influence. The method comprises the following steps: S1, constructing a pond microcosm system for dynamic analogue simulation; s2, putting the tested organism into the pond microcosm system, exposing the to-be-evaluated pollutants in the pond microcosm system, and after a period of time, analyzing to obtain the enrichment concentration of the to-be-evaluated pollutants in the water phase, the sediment and the tested organism; s3, analyzing and calculating the data to obtain the fugacity of the environmental phase and the fugacity capacity of the environmental phase; and S4, calculating a net change flux through a fugacity model, and evaluating the dynamic change of the pollutants in the pond microcosm system. According to the method, the experimental method is improved and the fugacity model is introduced, so that the defects of complexity, authenticity and analysis in the microcosm experiment in the prior art are effectively overcome, and a more perfect and accurate method is provided for deeply researching environmental behaviors of pollutants in a microcosm ecological system in a near-natural state.

The application discloses a kind of composite pollutant comprehensive toxicity effect determination method, comprising: constructing comprehensive toxicity model, obtaining the multidimensional behavior toxicity index for characterizing the change of test organism exposed to pollutants, construct comprehensive toxicity prediction model based on deep learning;With the multidimensional behavior toxicity index of the sewage to be tested as input, the comprehensive toxicity model is used to predict, and the comprehensive toxicity index prediction value is obtained, to determine whether the sewage to be tested exists toxicity effect.Compared with the traditional toxicity evaluation with death endpoint as criterion, the application is aimed at composite pollutants in sewage and actual sewage samples, while ensuring that the multidimensional behavior toxicity information of daphnia magna is fully characterized, the normalized expression of comprehensive toxicity index is realized, and the toxicity effect determination method based on ATI is established, which can be widely used in the comprehensive toxicity effect determination of composite pollutants.

The invention belongs to the field of experimental devices, and discloses a flexible heat insulation soft sheet type small water body temperature gradient experimental device and an experimental method.The experimental device comprises a water tank, the water tank has the length direction, the water tank extends in the length direction, an in-tank cavity is formed in the water tank, and the in-tank cavity is matched with the water tank; a heating unit and a refrigerating unit are arranged outside the two ends of the water tank respectively, and the heating unit and the refrigerating unit are started at the same time so as to heat and refrigerate water in the inner cavity of the water tank at the same time; a plurality of flexible heat insulation soft pieces are arranged in the in-groove cavity, the flexible heat insulation soft pieces are arranged in the in-groove cavity at equal intervals in the length direction, and a gap exists between the lower surface of each flexible heat insulation soft piece and the bottom of the in-groove cavity. The device is suitable for a small-size water body environment, the heating unit and the refrigerating unit provide a stable heating end and a stable cooling end at the same time, water temperature gradient and temperature constant boundary conditions are formed, hot water and cold water convection is achieved through the flexible heat insulation film and the gap, and the gap also gives consideration to water body communication and tested organism passing.

This invention relates to a low-dose aggregation-induced emission dye (AIE) bioimaging method, comprising the following steps: selecting a test organism and injecting it with a low dose of AIE; stimulating the test organism with an adjustable frequency excitation light source; acquiring fluorescence signals emitted from the test organism at equal time intervals; processing the fluorescence signals and generating a fluorescence image; applying phase-locked processing to the fluorescence image to separate the fluorescence signal component with the same frequency as the excitation light source and generating a phase-locked imaging result. By convolving the amplitude signal of each pixel in the fluorescence image with a sine or cosine signal with the same frequency as the excitation source, the orthogonality of trigonometric functions is utilized to effectively suppress noise, thus preserving the fluorescence signal that changes with the same frequency as the periodic light source. This significantly improves the signal-to-noise ratio of the fluorescence image, enabling clear observation of the AIE fluorescence signal even at low doses.

The utility model discloses a tracheal cannula comprehensive performance testing arrangement, including test organism, the inside embedded fixed water bath kettle body of test organism, the top of water bath kettle body is provided with the pot cover, and the inside of water bath kettle body is provided with the support mechanism, and the support mechanism includes fixed bolster and catheter positioning frame, and the one end of fixed bolster upper surface is rotatably connected in catheter positioning frame, and the one side surface of catheter positioning frame is arranged and fixed with catheter positioning clamp, and the below of pot cover is provided with elevating system, and elevating system includes elevating slide sleeve, and the inside sliding connection of elevating slide sleeve has elevating slide bar, the utility model discloses the design of catheter positioning frame support pot cover makes pot cover not to need to take down completely to realize the state of suspension of " to use " through the standing of catheter positioning frame. This design completely solved the problem that traditional separation type pot cover is difficult to place properly because of the compactness of test organism mesa space, and the operation process of pot cover taking is simplified.

1. Name of the product in this design: Chemical Detection and Analysis Instrument. 2. Purpose of this design: For testing chemical substances in biological samples. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The present disclosure relates to nucleic acid amplification devices for testing a bodily fluid sample for a biological analyte and their methods of use. Disclosed herein are nucleic acid amplification systems for untrained users or lay persons. The nucleic acid amplification devices have, e.g., a housing with valves, one or more incubation and detection chambers configured to enable the amplification and the detection of nucleic acids.

Summarizing the invention, a method is provided. The method comprises: accessing, by a computing device, test order data, wherein the test order data comprise information specifying a set of clinical tests to be carried out by a laboratory instrument on a biological sample contained in a sample container, wherein each clinical test is associated with a set of retain conditions so that the sample container is associated with one or more sets of retain conditions; determining, by the computing device, whether at least one set of retain conditions associated with the sample container is met; and if at least one set of retain conditions associated with the sample container is met, causing, by the computing device, the sample container to be retained in the laboratory instrument; wherein each set of retain conditions associated with a respective clinical test comprises a condition that one or more rules require that one or more supplementary tests shall be carried out whenever one or more supplementary test conditions on the respective clinical test are met.

The invention provides an ampullaria gigas-based prochloraz pesticide ecotoxicity detection method, which comprises the following steps: collecting or domesticating ampullaria gigas, and distinguishing individuals in a new hatching period and individuals in an adult period as test organisms; the method comprises the following steps: respectively exposing new hatching period pomacea canaliculata and adult period pomacea canaliculata in prochloraz aqueous solutions with different concentrations, exposing for 96 hours, and regularly observing and recording the survival individual number, the inhibited individual number and the individual number with escape behavior in each group during the period; the method comprises the following steps: exposing adult pomacea canaliculata in a series of prochloraz aqueous solutions with sublethal concentrations for 21 days, and regularly measuring and recording shell height and weight of the pomacea canaliculata bodies during exposure; taking the exposed hepatopancreas, kidney and stomach tissues of the ampullaria gigas, and carrying out histopathologic observation and analysis; the invention also provides application of the detection method in evaluating the ecotoxicity of the prochloraz pesticide. The pesticide toxicity can be rapidly and systematically tested through the easily obtained pomacea canaliculata, and the detection method is comprehensive and convenient to operate.

A method for high-throughput determination of whole water toxicity, including: exposing test organisms in a wastewater sample for pollution, obtaining phenotypic feature data of the test organisms; constructing a toxicity matrix; and building a machine learning model, and in combination with the toxicity matrix, determining a whole toxicity of the wastewater sample.

A lateral flow assay (LFA) device (10) for testing the presence of a target analyte in a biofluid sample comprises a longitudinal backing (12) having a hybrid sample / conjugate pad (18), a testing membrane (26) downstream the hybrid sample / conjugate pad (18) and an absorbent pad (34) downstream the testing membrane (26) extending thereon. The hybrid sample / conjugate defines a first treated section (18a) comprising a protein precipitating agent (20), a second treated section (18b) comprising a target analyte specific conjugate agent (22), and a third treated section (18c) comprising a flow assisting agent (24). The biofluid sample is collected by the hybrid sample / conjugate pad which provides for laminar flow of the biofluid sample through the first, second and third treated sections in sequence. The test membrane comprises detection lines (28) comprising respective detection agent for detecting the presence of the target analyte in the biofluid sample flowing thereon from the hybrid sample / conjugate pad (18) and into the absorption pad (34).

The present disclosure describes systems and methods for estimating the immobilization status of a test biological sample or any portion thereof treated with one or more immobilizing agents for morphological staining of an unknown duration. For example, the systems and methods of the present disclosure may estimate that the immobilization state of a morphologically stained test biological sample is one of "insufficient immobilization", "immobilized", or "over-immobilized".

A sample-testing system is disclosed. The system includes a separation system configured to separate a component of a sample for testing. The system further includes a housing enclosing the separation system. The housing includes a sample-receiving area configured to, when the housing is in a first configuration, receive the sample and direct the sample to the separation system, causing the separation system to separate the component of the sample. The housing further includes an extraction actuator configured to, when the housing is in a second configuration, extract the component of the sample from the separation system (and not extract the component when the housing is in the first configuration). The housing further includes a reconfiguration mechanism configured to reconfigure the housing from the first configuration to the second configuration.

The application provides an open field test data acquisition method and system based on three-dimensional space imaging principle, which comprises the following steps: placing two image acquisition devices with the same internal parameters beside a target open field, the two image acquisition devices being on the same horizontal plane and the optical axes of the two image acquisition devices being parallel; acquiring an open field three-dimensional image of the target open field through a 3D image imaging method and based on the configuration parameters of the two image acquisition devices; screening a biological image area of a test organism located in the target open field from the open field three-dimensional image; calculating a geometric centroid coordinate of the test organism according to the pixel coordinates and the total number of the area pixels in the biological image area; and acquiring open field test data based on the open field three-dimensional image and / or the geometric centroid coordinate. The application can quickly acquire open field test data and save manpower by automatically shooting images and calculating and analyzing image information.

A biological material testing apparatus (100) is provided that includes a substrate (102) having at least one chamber unit (104) defined therein, and a support member (106) for supporting a biological material contained in the at least one chamber unit. One of the substrate and the support member is molded on the other of the substrate and the support member. A method (10) for manufacturing such a biological material testing device is also provided. Uses and methods are also provided in which the device is used to test a biological material, for example in a drug test in which the biological material is exposed to a drug to be tested.

The present invention relates to a device for testing a biological sample and a method for determining a characteristic of a biological sample. The device for testing a biological sample comprises a receiving mechanism to receive a carrier, the carrier comprising holding areas, wherein the holding areas carry or have been exposed to the biological sample; a camera module configured to capture a collective image of the holding areas; and a processor configured to utilize the camera module to (1) identify visual cues located on the carrier from the captured collective image of the holding areas, and (2) based on the identification of the visual cues, perform an analysis process on the captured collective image; the analysis process comprising: dividing the captured collective image into a plurality of blocks, selecting a plurality of candidate blocks from the plurality of blocks, and determining a characteristic of the biological sample by analyzing the plurality of selected candidate blocks. The present invention can obtain the result of the test immediately and at a low cost.

Cyclic testing machine (100) for a specimen (11) of biological structures under cell culture conditions, the machine (100) comprising: - a base (1), - a crosspiece (2) hinged at one of its first end (2') by means of a cylindrical hinge (6), in turn integral with a first end (1') of the base (1), so as to operate a cyclic rotation motion around the cylindrical hinge (6), - a weight-holding basket (8) removably fixed on the crosspiece (2), - an anchoring cylinder (14) fixed to a second end (2') of the crosspiece (2), - a stepper motor (10) supported by a support (3), in turn integral with a second end (1'') of the base (1), - a pulley (15) keyed to one end of the stepper motor (10), said machine (100) being characterised in that - an inextensible cable (17) fixed to the anchoring cylinder (14) is wound in a groove (18) of the pulley (15), which rotates by defined angles by means of the stepper motor (10) and allows the movement of the crosspiece (2) itself by means of the hinge (6); - a pressing plate (12) integral with the crosspiece (2) configured to apply a compressive load to the specimen (11).

Existing techniques fail to provide a method to cumulate effects of interactions between groups of gut-associated microbes to predict efficiency of a probiotic organism in an individual. The present disclosure collects a test biological sample from the subject requiring personalization and extracts DNA from test biological sample and information specific to dietary preferences of the subject. Organisms from probiotic organisms dataset are obtained and a plurality of genome scale metabolic models are created for microbes comprised in gut microbiota of subject and obtained probiotic organisms. Metabolic simulations are performed to ascertain monoculture and co-culture growth of every pair of organisms comprised in gut microbiota of subject and obtained probiotic organisms. Sustainability is computed for evaluating capability of each organism to proliferate within gut. Net-effect is computed by quantifying an overall influence of each probiotic organism. An efficacious probiotic organism is selected based on at least one of net-effect and sustainability.

The application discloses a kind of water quality comprehensive toxicity high flux determination method, the determination method includes using sewage sample to expose the test organism after being contaminated, obtains the phenotypic characteristic data of test organism;Toxicity matrix is constructed;Establish machine learning model, determine the water quality comprehensive toxicity of sewage sample in combination with toxicity matrix.The water quality comprehensive toxicity determination method provided by the application does not need sample concentration enrichment, guarantees the comprehensiveness and accuracy of detection result, and can be applied to the comprehensive toxicity high flux determination of sewage treatment plant whole-process sewage.

The application discloses a method for determining a seawater ecological risk evaluation threshold of microplastic particles, and comprises the following steps: (1) preparing pure microplastics, (2) biological testing, and (3) threshold derivation. Through the method, the toxic effect of pure microplastics can be determined instead of the combined toxic effect of microplastics and leaching additives, so that the toxicity of microplastic particles to marine organisms can be more accurately evaluated. In addition, the tested organisms used in the method are all marine organisms, so that the risk of microplastic particles to seawater ecology can be more accurately reflected. The method can also derive the toxicity threshold of microplastic particles to marine organisms.