Microbial aggregate quantitative microscopic imaging testing and evaluating method

A technology of microbial aggregates and evaluation methods, applied in the field of comprehensive evaluation of the performance of microbial aggregates, can solve the problems of single-factor evaluation of residence and the lack of accurate quantitative comprehensive evaluation methods for microbial aggregates.

Inactive Publication Date: 2013-08-07
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are also studies that try to use a single indicator for the quantitative characterization of microbial aggregates, such as individual size, etc., but they all stay in the aspect of single-factor evaluation
At present, there is no comprehensive evaluation method for accurate quantification of microbial aggregates.

Method used

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  • Microbial aggregate quantitative microscopic imaging testing and evaluating method
  • Microbial aggregate quantitative microscopic imaging testing and evaluating method
  • Microbial aggregate quantitative microscopic imaging testing and evaluating method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Research on the performance of microbial aggregates in aquaculture wastewater

[0049] 1. Preparation of microbial aggregates to be tested: Prepare a 1L aerobic batch reactor, inoculate 25mL of sludge, add 1.90g of sucrose, NH 4Cl is 0.0764g simulated aquaculture wastewater to a volume of 755mL. Put the aeration head into the above-mentioned reactor to carry out the aeration experiment, and take a sample at 3 hours after the experiment, as the microbial aggregate to be tested.

[0050] 2. Performance test of microbial aggregates to be tested

[0051] a. Calibrate the standard relationship curve between the gray value and the specific gravity value of the microbial aggregate sample to be tested. The specific steps are as follows:

[0052] a1. Taking 1.0°Bx as the sugar content gradient, 17 groups of sucrose aqueous solutions with a sugar content ranging from 5.0°Bx to 21.0°Bx were taken, marked as sucrose aqueous solution m, 1≤m≤17;

[0053] a2, take the mi...

Embodiment 2

[0094] Example 2: Test aeration time on performance impact of microbial aggregates in aquaculture wastewater

[0095] 1. Preparation of microbial aggregates to be tested: Prepare a 1L aerobic batch reactor, inoculate 25mL of sludge, add 1.90g of sucrose, NH 4 Cl is 0.0764g simulated aquaculture wastewater to a volume of 755mL. Put the aeration head into the above-mentioned reactor to carry out the aeration experiment, and take samples at the 3h, 6h, 9h, 12h, 24h, 48h, 72h and 96h of the experiment, as 8 groups of microorganisms to be tested body.

[0096] 2. Analyze the sample taken during 3 hours, repeat step 2 a and b of Example 1 to obtain the area and specific gravity values ​​of n microorganism aggregates to be tested, calculate the average value of the area and specific gravity respectively, and record it as x 11 and x 21 . According to the same method, calculate the average area and average specific gravity of the microbial aggregates to be tested in the samples of ...

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Abstract

The invention discloses a quantitative microscopic imaging testing and evaluating method of microbial aggregate property. The method is characterized by comprising the following steps: firstly, demarcating a standard relation curve of grey scale values and specific gravity values of a to-be-tested microbial aggregate sample; then obtaining an image of a to-be-tested slide made of a mixed solution containing to-be-tested microbial aggregates by using a digital microscope, calculating grey scale values and areas of all the to-be-tested microbial aggregates from resolutions, R values, G values and B values of all the microbial aggregates in the image, and calculating specific gravity values from the grey scale values as well as the standard relation curve of grey scale values and specific gravity values; and finally performing comprehensive evaluation on the areas and the specific gravity values of the to-be-tested organism aggregates by using a fuzzy pattern recognition method, to obtain a level characteristic value vector as an evaluation result of the to-be-tested organism aggregates. According to the high-efficiency and precise method, quantitative analysis and comprehensive evaluation on the microbial aggregates are achieved.

Description

technical field [0001] The invention relates to a method for measuring and evaluating the performance of microbial aggregates in biological treatment, in particular to a method for measuring and comprehensively evaluating the performance of microbial aggregates using quantitative microscopic imaging technology and fuzzy pattern recognition. Background technique [0002] Microbial aggregates mainly appear in the form of activated sludge, biofilm, granular sludge, etc., and are the main body of sewage biological treatment. The performance of microbial aggregates determines the effect of sewage purification and solid-liquid separation. [0003] In the process of sewage biological treatment, bacteria, fungi, protozoa and other microorganisms reproduce in large quantities, plus organic and inorganic colloids and suspended solids in sewage to form a flocculent mud particle that is visible to the naked eye, which is a microbial aggregate. It has strong adsorption capacity and degra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N9/24
Inventor 陈国炜汤泽兵刘欢梁腾方祝宁刘丽胡真虎
Owner HEFEI UNIV OF TECH
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