Microbial aggregate quantitative microscopic imaging testing and evaluating method
A technology of microbial aggregates and evaluation methods, applied in the field of comprehensive evaluation of the performance of microbial aggregates, can solve the problems of single-factor evaluation of residence and the lack of accurate quantitative comprehensive evaluation methods for microbial aggregates.
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Embodiment 1
[0048] Example 1: Research on the performance of microbial aggregates in aquaculture wastewater
[0049] 1. Preparation of microbial aggregates to be tested: Prepare a 1L aerobic batch reactor, inoculate 25mL of sludge, add 1.90g of sucrose, NH 4Cl is 0.0764g simulated aquaculture wastewater to a volume of 755mL. Put the aeration head into the above-mentioned reactor to carry out the aeration experiment, and take a sample at 3 hours after the experiment, as the microbial aggregate to be tested.
[0050] 2. Performance test of microbial aggregates to be tested
[0051] a. Calibrate the standard relationship curve between the gray value and the specific gravity value of the microbial aggregate sample to be tested. The specific steps are as follows:
[0052] a1. Taking 1.0°Bx as the sugar content gradient, 17 groups of sucrose aqueous solutions with a sugar content ranging from 5.0°Bx to 21.0°Bx were taken, marked as sucrose aqueous solution m, 1≤m≤17;
[0053] a2, take the mi...
Embodiment 2
[0094] Example 2: Test aeration time on performance impact of microbial aggregates in aquaculture wastewater
[0095] 1. Preparation of microbial aggregates to be tested: Prepare a 1L aerobic batch reactor, inoculate 25mL of sludge, add 1.90g of sucrose, NH 4 Cl is 0.0764g simulated aquaculture wastewater to a volume of 755mL. Put the aeration head into the above-mentioned reactor to carry out the aeration experiment, and take samples at the 3h, 6h, 9h, 12h, 24h, 48h, 72h and 96h of the experiment, as 8 groups of microorganisms to be tested body.
[0096] 2. Analyze the sample taken during 3 hours, repeat step 2 a and b of Example 1 to obtain the area and specific gravity values of n microorganism aggregates to be tested, calculate the average value of the area and specific gravity respectively, and record it as x 11 and x 21 . According to the same method, calculate the average area and average specific gravity of the microbial aggregates to be tested in the samples of ...
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