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Method for measuring content of ayfivin and its preparations by nephelometry

A technology of bacitracin and turbidity method, which is applied in the field of determination of antibiotic content, can solve problems such as high requirements for antibiotic time control, difficult operation control, and error in measurement results, so as to shorten the cultivation time, simplify the measurement method, increase the The effect of oxygen supply

Inactive Publication Date: 2008-09-24
高燕霞
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Problems solved by technology

As a result, the tube-and-disk method requires high time control and takes a long time when determining the content of antibiotics.
Therefore, using the tube-and-disk method to determine the content of antibiotics such as bacitracin has the following disadvantages: the operation is not easy to control, the error of the measurement result is large, and the measurement speed is slow, which makes it have great application limitations in industrial production.

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  • Method for measuring content of ayfivin and its preparations by nephelometry

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Experimental program
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Embodiment Construction

[0028] Experimental materials: The test bacteria adopt Staph. Epidermidis ATCC12228 publicly sold by the American Standard Bacteria Bank, and the concentration of the bacteria solution is 6.0%-8.0%; No. III pH=7.0-7.2 antibiotic testing culture medium publicly sold by the product testing institute, the sterilizing buffer is a phosphate buffer solution of pH=6.0, and its preparation method is: get dipotassium hydrogen phosphate 2g and potassium dihydrogen phosphate 8g, add water to make 1000ml, filter, sterilize at 115°C for 30 minutes, set aside;

[0029] Experimental procedure:

[0030] (1) Preparation 1 of standard solution (one-dose method): Accurately weigh 2.5 mg of bacitracin standard substance, put it in a 25ml measuring bottle, add 0.1M hydrochloric acid solution to dissolve and dilute to the mark, shake well, and make every 1ml containing The stock solution of the solution of bacitracin 100ug; accurately measure 5ml of the above stock solution, put it in a 50ml measu...

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Abstract

The invention discloses a method for measuring bacitracin and the content of the preparation thereof by the turbidity method, and the method contains the following steps: the preparation of standard solution, the preparation of test solution, the preparation of test organism suspension, the content measurement, etc. The antibiotics-microbial test and multi-dose turbidity method of the invention are adopted for measuring the bacitracin and the content of the preparation thereof, thus the test method is accurate and effective and has low cost, and test results can truly reflect antibacterial activity of the bacitracin and the preparation thereof; the method has stronger practicality and operability in the production and the quality control of the drug and the preparation thereof.

Description

technical field [0001] The invention relates to a method for determining the content of antibiotics, in particular to a method for determining the content of bacitracin and its preparations by turbidimetric method. Background technique [0002] The producing bacteria of Bacitracin are Bacillus subtilis and Bacillus licheniformis, which are polypeptide multicomponent antibiotics containing thiazole ring. Bacitracin can inhibit the formation of linear peptidoglycan chains, the main component of bacterial cell walls, and also affect protoplasts, destroy serosa, etc. It has a high degree of antibacterial activity against Gram-positive bacteria, and bacteria rarely develop acquired resistance to this product It has synergistic effect with combined topical application of neomycin (Neomycin) and polymyxin B (Polymyxin B). At present, bacitracin raw materials and their preparations are included in the second part of the "Chinese Pharmacopoeia" 2005 edition, and the method for deter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/17
Inventor 高燕霞姜建国张西如王茉莉韩彬
Owner 高燕霞
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