141 results about "Bacitracin" patented technology

The invention relates to a Chinese herbal medicine biological feed additive for livestock and a preparation method of the Chinese herbal medicine biological feed additive. The prepared Chinese herbal medicine biological feed additive for the livestock comprises the following raw materials in percentage by mass: 20% of probiotics, 10% of an enzyme preparation, 50% of traditional Chinese medicines, 10% of amino acids, 4% of vitamins, 1% of micro elements, 1% of an immunopotentiator, 1% of bacitracin and 3% of glucose. The preparation method comprises the following steps: firstly carrying out expanding culture to revive the probiotics, fermenting the probiotics for 2 days so as to obtain probiotics fermentation liquor; placing the traditional Chinese medicines into the probiotics fermentation liquor for fermentation; and finally mixing the dried fermentation products with other raw materials so as to obtain the Chinese herbal medicine biological feed additive for the livestock. The prepared Chinese herbal medicine biological feed additive for the livestock disclosed by the invention has the advantages of improving the immunity, increasing the utilization rate of the feed, improving environment and the like.

The invention relates to the fields of analytical chemistry and food safety, particular to a method for detecting the residual quantity of multiple polypeptide veterinary drugs in animal-derived foods. The method comprises the following steps of: processing a sample with a TCA(trichloroacetic acid) and acetonitrile system for depositing proteins; extracting with a carbinol and 0.1% formic acid aqueous solution system; purifying with a Oasis HLB solid phase extraction column; performing gradient elution with an Eclipse XDB-C18 analytical column in the presence of an acetonitrile and 0.1% formic acid aqueous solution used as a mobile phase; and then performing electrospray and positive ion scanning mode separation for finally detecting four polypeptides. The limits of the four polypeptides, namely colistin, bacitracin A, polymyxin B and virginiamycin M, are 25 micrograms/kilogram, 100 micrograms/kilogram, 250 micrograms/kilogram and 120 micrograms/kilogram respectively; the recovery rates of colistin, bacitracin A, polymyxin B and virginiamycin M are 74.9-88.1%, 76.2-89.0%, 76.6-81.2% and 77.3-86.9% respectively; and the coefficients of variation (CV%) of colistin, bacitracin A, polymyxin B and virginiamycin M are 5.7-15.1%, 7.2-15.7%, 6.0-8.0% and 9.5-18.6% respectively. The detection limit, the recovery rate, the accuracy and other technical indexes all meet related detection requirements at home and aboard.

The invention relates to a beaver rabbit feed, which belongs to the feed field. The beaver rabbit feed mainly comprises the components of robenidine hydrochloride, monensin, salinomycin, bacitracin, garlicin, feed yeast, feed grade magnesium sulphate, feed grade DL-methionine, feed grade L-lysine hydrochloride, common salt, sulfur, calcium hydrophosphate, copper element, zinc element, iron element, choline chloride and vitamin complex premix, etc. The beaver rabbit feed can ensure that the conception rate of female beaver rabbits is improved to more than 93 percent and even to 100 percent; by adopting the beaver rabbit feed, the survival rate at 60 days of the beaver rabbits in the growing period can be improved to about 95 percent, and the growth cycle can be reduced by about half a month; simultaneously the growth of the hair of the beaver rabbit can be promoted, and the density, thickness uniformity and color brightness of the hair can be improved.

The invention relates to a method for extracting and separating bacitracin. The method is characterized by comprising the following steps of: (1) extracting and separating bacitracin concentrated liquor by ultrafiltration, namely acidizing bacitracin fermentation liquor, filtering or centrifuging by a vibrating screen to remove thalli, removing the thalli and macromolecular impurities by the ultrafiltration, removing an inorganic salt by resin adsorption and performing evaporation concentration under vacuum to obtain the bacitracin concentrated liquor; and (2) preparing a bacitracin crystalline solid by using the bacitracin concentrated liquor, namely performing production steps of extracting, decolorizing, nanofiltration, crystallizing and separating, drying, crushing, sieving, checking, the packaging of a finished product and the like on the bacitracin concentrated liquor to obtain the qualified bacitracin product. The method has the characteristics of high efficiency, simple equipment, high economy, large separation factor, high selectivity, low energy consumption, no secondary pollution, and the like, and is particularly suitable for the large-scale industrial production of the bacitracin; and operation can be performed continuously at the room temperature.

The invention relates to a method for increasing titer of bacitracin in fermentation liquid by using oxygen carrier. A certain amount of oxygen carrier is added into a fermentation medium to improve the oxygen dissolution condition and greatly increase titer of bacitracin fermentation liquid, without changing other fermentation conditions. The method has simple process; the oxygen carrier in the production process can be recycled, so as to reduce production cost; therefore, the method has an important guiding significance for antibiotic production.

The invention discloses a preparation method and an application of a feeding bacillus licheniformis (Baclicuslincheniformis) inoculant, and belongs to the field of feeder additives. The bacillus licheniformis BLBY-7 is selected from rumen of a cattle, which can secrete bacitracin, has a broad-spectrum antibacterial effect in intestinal tracts of livestock and poultry, can directly destroy pathogenic bacteria, leaves no toxin, and also can secrete high-activity protease, thus improving the digestibility and utilization rate of feed; and the inoculant contains more than 8-23*10<10>CFU/g of bacillus licheniformis, which is added to the general feed of livestock and poultry based on 0.01 to 0.05% by weight, and can effectively improve the resisting capacity of livestock and poultry to general diseases, as well as reduce the usage amount of antibiotics.

The invention belongs to the field of animal bactericides, and discloses a bactericide containing aloe extract and a preparation method for the bactericide. The bactericide comprises the following components: the aloe extract, essential oil, a cationic bacteriostatic agent, a surface active agent and water. The bactericide further comprises at least one of bacteriostasis synergist, a thickener, adeodorant, bacitracin or honeysuckle extract. Since the bactericide prepared by the invention contains the aloe extract, especially aloe peel extract, plant essential oil and cationic bacteriostatic agent, the bactericide has good bactericidal and bacteriostatic effects, and the bactericide has no pungent smell; and the antibacterial agent further has the effects of removing peculiar smell, soothing furs and the like on animals, and cannot cause damage to animal hairs or skin, and is non-toxic, non-irritating, non-corrosive, safe and environment-friendly.

The invention relates to a bacillus licheniformis with a vitreoscilla hemoglobin gene. The bacterial strain is the bacillus licheniformis (PHY300-vgb), wherein the bacterial strain is stored in China Typical Model Cultivation Center of Wuhan, and the storage number is CCTCC NO: M2011345. The invention aims to provide the bacterial strain of the bacillus licheniformis with vitreoscilla hemoglobin gene, which can produce bacitracin in high yield, as well as a construction method and an application of the bacterial strain.

The invention provides a composite hydrogel wound dressing and a preparation method thereof. The aminated collagen and oxidized sodium alginate are used as base materials; two polypeptide antibiotics,namely polymyxin B sulfate and bacitracin are loaded; and the AC/OSA-PB composite hydrogel dressing is prepared through a Schiff base reaction. The preparation method is simple to operate; the requirements on reaction conditions are low; gel can be formed in a short time, large-scale production is facilitated, and the prepared wound dressing not only has a good bactericidal effect, but also can improve the HSF cell mobility and the HSF cell proliferation rate to the maximum extent, has excellent wound healing promoting capacity, and also has mechanical properties and mechanical properties suitable for skin wounds.

The invention provides a method of constructing bacillus licheniformis by knocking out a ccpN gene, a strain and application of the strain. By adopting a gene engineeringmethod, the bacillus licheniformis DW2 delta ccpN is constructed by knocking out the ccpN gene in a bacillus licheniformis DW2 genome; and compared with the bacillus licheniformis DW2, for the strain, the yield of bacitracin is increased by 16 percent or above.

The present invention pertains to compositions and methods used to ascertain if putative antibiotics trigger a cell envelope stress response in bacterial organisms. In one aspect of the invention, three two-component systems are described: LiaR & LiaS (formerly YvqCE); YvcP & YvcQ; and BceR & BceS (formerly YtsAB). These systems, for example, can be used to analyze antibiotics such as vancomycin, bacitracin, nisin, and ramoplanin, which interfere with the lipid II cycle in the cytoplasmic membrane.

The invention aims to provide pharmaceutical grade bacitracin suitable for various raw material sources and a preparing device thereof. The preparation method of the pharmaceutical grade bacitracin comprises the steps of acidizing bacitracin fermentation broth, and conducting filtration with a ceramic membrane; conducting cation exchange chromatography on ceramic membrane dialysate, and collecting cation exchange chromatography column-loading liquid; conducting anion exchange on the cation exchange chromatography column-loading liquid, and collecting anion exchange column-loading liquid; conducting metal-chelating on the anion exchange column-loading liquid, and collecting metal-chelating column liquid; conducting nanofiltration on the metal-chelating column liquid, so that final nanofiltration product liquid is obtained; dissolving and filtering the final nanofiltration product liquid, and drying and smashing filtrate, so that the pharmaceutical grade bacitracin is obtained. The initial substance bacitracin fermentation broth can be replaced with low-content bacitracin solution, and the molecular weight cut-off of a nanofiltration membrane for final nanofiltration is 300-500 Da.

The invention discloses a bacillus licheniformis CPL618 as well as screening and application thereof. The strain CPL618 is identified as bacillus licheniformis, the strain CPL618 is preserved in the China Center for Type Microbiological Culture Collection (CCTCC), the preservation time is September 13, 2021, and the preservation number is CCTCC NO: M20211158. The bacillus licheniformis CPL618 disclosed by the invention has broad-spectrum antibacterial activity, and can antagonize the growth of gram-positive bacteria, gram-negative cocci and fungi. The invention provides an optimized industrial culture medium which can improve the yield of bacitracin A at lower cost, and the maximum yield can reach 2.3 g/L. The bacillus licheniformis disclosed by the invention has a wide utilization prospect in the aspects of preparation of antibacterial agents and production of bacitracin A.

The invention discloses a method for measuring bacitracin and the content of the preparation thereof by the turbidity method, and the method contains the following steps: the preparation of standard solution, the preparation of test solution, the preparation of test organism suspension, the content measurement, etc. The antibiotics-microbial test and multi-dose turbidity method of the invention are adopted for measuring the bacitracin and the content of the preparation thereof, thus the test method is accurate and effective and has low cost, and test results can truly reflect antibacterial activity of the bacitracin and the preparation thereof; the method has stronger practicality and operability in the production and the quality control of the drug and the preparation thereof.

The invention relates to the field of gene engineering and microbial metabolism engineering, discloses promoters suitable for bacillus licheniformis and application of the promoters in efficient expression of a target product, and artificially designs four promoters (R1-R4) suitable for bacillus licheniformis. Then, green fluorescent protein GFP and keratinase KER are taken as target proteins, andcompared with a promoter P43, research finds that the promoter R2 has the best effect, the expression levels of GFP and KER are remarkably improved, the yield of GFP is improved by 63.5 times, and the enzyme activity of KER is improved by 7.8 times. Finally, the promoter R2 is applied to metabolic engineering, and compared with a control strain, the yield of poly gamma-glutamic acid and the yieldof bacitracin are increased by 52.09% and 20.39% respectively by applying the promoter R2 to metabolic pathway strengthening.

The present invention relates to the application of bacitracin A in preparation of a medicine for preventing and treating corona virus, and especially provides application of the bacitracin A or pharmaceutically acceptable salt thereof, isotope, stereoisomers, stereoisomer mixture, tautomer, ester, amide or prodrug in preparation of a medicine for preventing and/or treating diseases caused by thecorona virus. The corona virus is novel corona virus SARS-Cov-2, SARS-CoV, HCoV 229E, NL63, OC43, HKU1 and MERS-CoV. The median effective concentration (EC50) of the bacitracin A to the new corona virus (SARS-Cov-2) is 2.38 [mu]M, and the toxicity is low, which indicates that the bacitracin A has a good therapeutic window.

The invention discloses a method for improving the bacitracin valence through continuous flowing fed-batch fermentation. The method comprises the following steps: a, performing slant culture: inoculating bacillus licheniformis into a slant culture medium, and culturing for 24 hours at 37 DEG C; b, preparing bacterium suspension: preparing the slant strain prepared in the step a into bacterium suspension by using sterile water; c, performing one-class seed culture: inoculating the bacterium suspension prepared in the step b into a one-class seed jog with a seed culture medium, and culturing for 16 hours under the conditions that the temperature is 37 DEG C, the rotation speed is 600rpm and the gas supply amount is 0.5vvm; d, performing fed-batch fermentation: inoculating the one-class seeds prepared in the step c into a fermentation tank with a fermentation culture medium according to a volume ratio of 10%, fermenting under the conditions that the temperature is 37 DEG C, the rotation speed is 600rpm and the gas supply amount is 1.0vvm, when the strain enters into an index growth period, starting supplementing materials at uniform speed, after the supplementation, controlling the pH value to be 7.0-7.5, and stopping fermentation after 40 hours; and e, detecting fermentation unit: detecting the fermentation unit of bacitracin, and comparing the fermentation unit of bacitracin with the fermentation unit of the bacitracin without fed-batch fermentation in production.

The invention relates to marine organism antibacterial fabric softener, which is applicable to post-wash caring of various clothing and enables bacteria removal and softening to be simultaneously completed. After the marine organism antibacterial fabric softener is used, clothing is as bright as new, soft, smooth, neat and tight-fitting and has an antistatic effect. The marine organism antibacterial fabric softener added with marine organism antibacterial ingredients is nontoxic, non-irritant and capable of efficiently removing bacteria on the clothing, such as staphylococcus aureus, escherichia coli and candida albicans. The marine organism antibacterial fabric softener has the advantages that the softener is harmless to fabrics and highly stable, the softener made according to a phosphate-free aluminum-free neutral formula is environmentally pollution-free and harmless to skin, and the softener is simple in production process, low in cost and the like. The formula of the marine organism antibacterial fabric softener includes (by weight) 6.0-8.0% of marine organism prebiotics, 0.5-1.0% of bacitracin, 8.0-12.0% of palm anodic ester-based quaternary ammonium salt, and the balance of pure water.

The invention discloses a sewage treatment material. The sewage treatment material is prepared from the following raw materials in parts by weight: 88 to 102 parts of polyacrylamide powder, 6 to 8 parts of ethylenediamine tetraacetic acid powder, 69 to 87 parts of activated clay, 50 to 70 parts of activated carbon powder, 101 to 122 parts of chitin, 8 to 10 parts of hydroxyapatite powder, 7 to 10parts of bacitracin, 11 to 15 parts of glutathione, 13 to 15 parts of silymarin, 7 to 12 parts of sodium pyrophosphate powder, 17 to 20 parts of arabic gum powder, 2 to 4 parts of bee wax, 3 to 6 parts of sodium carboxymethyl starch, 6 to 9 parts of sodium bicarbonate powder, 7 to 10 parts of sodium alginate and 18 to 24 parts of croscarmellose sodium powder. The sewage treatment material has an excellent treatment effect on sewage containing complicated components.

The invention provides a bacillus licheniformis strain capable of realizing high production of bacitracin as well as a preparation method and application thereof. Bacillus licheniformis DW2-ilvB is obtained through a genetic engineering method and by inserting ilvB genes in a genomic DNA (Deoxyribonucleic Acid) sequence of bacillus licheniformis DW2; relative to the bacillus licheniformis DW2, theyield of the bacitracin in a fermentation solution during a bacitracin fermentation process of the strain is increased by 11 percent or more.

The invention provides a low-peculiar smell bacitracin production bacillus licheniformis and a construction method and an application of the low-peculiar smell bacitracin production bacillus licheniformis. A ptb gene in a genomic DNA sequence of the bacillus licheniformis DW2 is knocked out through a genetic engineering method to obtain the bacillus licheniformis DW2deltaptb, and compared with the bacillus licheniformis DW2, the synthetic amounts of isobutyric acid and isovaleric acid of the bacterial strain are respectively reduced to 33% and 37% in the fermentation process of the bacitracin, and the peculiar smell is remarkably reduced.

The invention provides an ointment preparation as well as a preparation method and application thereof, belongs to the technical field of medicine preparations, and is used for solving the problem that a conventional skin injury medicine cannot have take effects of bleeding stopping, pain relieving, infection prevention and the like into account all generally. The ointment preparation comprises a matrix, polymyxin B sulfate, neomycin sulfate, bacitracin and hemostatic, wherein the polymyxin B sulfate, the neomycin sulfate, the bacitracin and the hemostatic are uniformly dispersed in the matrix. Due to synergism of multiple antibiotics in the ointment preparation, not only is a complementary antibacterial spectrum provided, but also the antibacterial range is widened, most skin bacterial infection pathogenic bacteria are covered, and moreover, due to the synergistic and overlapping effect of the antibiotics, the antibacterial function can be enhanced, and induced drug resistance can be avoided. In addition, the ointment preparation comprises local anesthetic and hemostatic, so that a prevention and treatment effect on partial skin bacterial infection can be achieved, functions of pain relieving and bleeding stopping of partial skin wound can be achieved, and patients can be greatly relieved from pain.