35 results about "Bacitracin" patented technology

The invention belongs to the technical field of food and feed analysis and detection, and particularly provides a nano antibody for detecting bacitracin methylene salicylate, and the nano antibody comprises a nano antibody BMD-BA19 and a nano antibody BMD-CA45; the amino acid sequence of a VHH chain of the nano antibody BMD-BA19 is as shown in SEQ ID NO: 1, and the amino acid sequence of a VHH chain of the nano antibody BMD-CA45 is as shown in SEQ ID NO: 2. The bacitracin methylene salicylate serves as a research object, after the bacitracin methylene salicylate is coupled with protein, an alpaca is immunized, two nano antibodies resisting bacitracin methylene salicylate are obtained through screening with a phage display technology, and a material is provided for establishing a bacitracin methylene salicylate detection method.

The invention belongs to the field of gene engineering and enzyme engineering, and discloses a phosphoketolase mutant derived from bifidobacterium adolescentis and application of the phosphoketolase mutant in production of metabolites. By adopting a site-specific mutagenesis method, histidine at the 480th site of the phosphoketolase PKT from bifidobacterium adolescentis is mutated into lysine, so that the enzyme activity of the phosphoketolase is remarkably improved, and the problem that the existing phosphoketolase has relatively low catalytic performance on 6-phosphofructose is effectively solved; the strain is successfully applied to fermentation production of bacitracin, ectoine and gamma-aminobutyric acid, and a new thought is provided for improving the production level of metabolites.

The application provides application of bacitracin or a composition of the bacitracin and oregano essential oil in improving meat quality of ruminants, and relates to the technical field of animal feed. The bacitracin is used as an active ingredient for preparing feed, and the addition amount of the bacitracin in the feed is 1.0-2.4 g / kg. The active ingredient also comprises oregano essential oil, and the mass ratio of the bacitracin to the oregano essential oil is 3.5-4.0:1. The bacitracin can be used for improving meat quality of ruminants for the first time, and when the bacitracin is used in combination with the oregano essential oil, synergistic effects can be produced in meat color, tenderness, fatty acid composition and formation of volatile flavor substances, muscle quality of different parts is improved, meat quality is effectively improved, the comprehensive commodity value of ruminant meat is greatly improved, and the bacitracin has a good popularization prospect.

The present application provides a bacitracin-producing engineering bacterium with oxdC gene knocked out and a preparation method and application thereof, which adopts a genetic engineering means to knock out an oxdC gene in a genome of Bacillus licheniformis, and obtains a recombinant strain after the oxdC gene is knocked out, namely the bacitracin-producing engineering bacterium, and the nucleotide sequence of the oxdC gene is shown in SEQ ID NO. 1. The bacitracin-producing engineering bacterium has stronger fermentation production capacity and higher yield, and the bacitracin yield is increased by more than 16% compared with Bacillus licheniformis DW2.

The invention provides Kineococcus G2 and application of the Kineococcus G2 in preparation of a pigment with antioxidant activity, the Kineococcus new strain G2 is CCTCC No. M20242602, the strain is spherical, the size is 1.0 * 1.5 mu m, the optimal growth temperature is 30-37 DEG C, the pH value is 7.0-9.0, and the salt concentration is 1.0%-2.0%. The strain G2 is not sensitive to bacitracin, ciprofloxacin and norfloxacin, and is sensitive to novobiocin, polymyxin, gentamicin, piperacillin, ofloxacin, erythromycin, streptomycin, kanamycin, rifampicin, ampicillin, vancomycin, carbenicillin, chloramphenicol, tetracycline, penicillin, cefoperazone, oxacillin, amoxicillin and neomycin. Pigment generated by fermentation of the G2 strain has good antioxidant activity, and when the concentration is 10 micrograms / mL, the scavenging activity on DPPH is 25.00%, the scavenging activity on hydroxyl radicals is 80.58%, and the iron ion reducing capacity is 1.57, which are all higher than those of contrast beta-carotenoid. The application of the new Kineococcus strain G2 can lay a foundation for the research of antioxidant drugs such as medical treatment and health care product development.

This invention relates to microbial culture. It provides a *Bacillus parabregas* culture medium and its applications, wherein the medium comprises at least one of glycerol, yeast extract, soybean peptone, corn steep liquor powder, ammonium sulfate, magnesium sulfate, and calcium carbonate. The *Bacillus parabregas* culture medium of this invention utilizes glycerol as a carbon source and yeast extract, soybean peptone, etc., as nitrogen sources for large-scale liquid fermentation of *Bacillus parabregas*, achieving a total bacitracin content of over 10.5 g / L. The proportions of each component can be controlled by feeding. Using this medium for *Bacillus parabregas* fermentation results in high bacitracin yield and controllable composition. The medium formulation is simple, inexpensive, readily available, safe, and environmentally friendly. The fermentation process is simple and controllable, with a short fermentation cycle, facilitating industrial-scale production and possessing high application value.

The present invention belongs to the fields of genetic engineering and enzyme engineering, and discloses a phosphoketolase mutant derived from Bifidobacterium adolescentis and its application in the production of metabolites. The present invention uses a site-directed mutagenesis method to mutate the 480th histidine of the phosphoketolase PKT derived from Bifidobacterium adolescentis to lysine, significantly improving the enzyme activity of the phosphoketolase. This effectively solves the problem of the current low catalytic performance of phosphoketolase on fructose-6-phosphate. The enzyme has been successfully applied to the fermentation production of bacitracin, ectoine, and γ-aminobutyric acid, providing a new approach to improving the production level of metabolites.

The present invention discloses a method for detecting bacitracin in compound polymyxin B ointment. In the present invention, the compound polymyxin B ointment is heated and melted, n-hexane is added to disperse the ointment body evenly, then ammonia water solution is added for extraction, the extraction liquids are combined, the pH value is adjusted to 7.0, and then chromatographic detection is carried out under improved chromatographic conditions. For the pretreatment method of the present invention, the recovery rate of bacitracin is high, close to 100%, the types and contents of impurities have no difference from those of the reference substance, which can meet the detection requirements and can be used for the quality control of bacitracin in compound polymyxin B ointment.

The invention discloses a purification method and application of bacitracin A, and relates to the field of biology. According to the method for purifying the bacitracin A, preparation of the high-purity bacitracin A is achieved through liquid chromatographic separation, the purity of the bacitracin A can reach 95% or above, the separation and purification process of the high-purity bacitracin A is simplified to a great extent, the loss of reagents in the separation and purification stage is reduced, and the yield of the high-purity bacitracin A is increased. The method has the advantages of high separation purity, less organic solvent dosage, simple device, simple and convenient process operation, environmental protection and the like, and is suitable for process production.

The invention discloses a method for improving the fermentation level of bacitracin, and belongs to the technical field of veterinary drug production. A method for improving the fermentation level of bacitracin comprises the following steps: preparing a plurality of sterile fermentation tanks including a main fermentation tank, at least one propagation tank and a primary fermentation tank, inoculating a seed solution containing bacitracin producing bacteria into the primary fermentation tank, and inoculating the seed solution with thalli entering a logarithmic phase into the main fermentation tank sterilized in advance. According to the method for improving the fermentation level of bacitracin provided by the invention, the main fermentation tank is used for expanding culture towards the expanding culture tank, so that the dissolved oxygen of feed liquid in the fermentation tank can be effectively increased, the metabolic activity of hyphae is improved, the titer growth is further promoted, the concentration of bacitracin in unit volume of fermentation liquid is remarkably improved, and the fermentation titer is improved; part of feed liquid in the main fermentation tank is distributed into the expanding culture tank for culture, and the liquid level is lowered, so that the use of a defoaming agent can be reduced, the energy consumption and the raw material consumption are reduced, and the production cost is reduced.

The invention relates to the technical field of batteries, in particular to a diaphragm, a preparation method thereof and a battery. The separator comprises: a base film; the coating is arranged on the surface of at least one side, in the thickness direction, of the base film, and the coating comprises composite fibers; the composite fiber comprises a compound formed by dehydrating and condensing a reactant A and a reactant B, wherein the reactant A comprises at least one of bacitracin or a bacitracin metal chelate, and the reactant B comprises at least one of a hydroxyl-containing polymer, a carboxyl-containing polymer, an acylamino-containing polymer or an amino-containing polymer. Wherein the reactant A and the reactant B are stably combined through a covalent bond, and a uniform and compact coating is formed on the interface of the base membrane in an electrostatic spinning manner. The diaphragm has excellent softness, relatively high tensile strength, good hydrophilicity and slow release characteristic, and also has the characteristics of stability, heat resistance, excellent ionic conductivity and the like.

The invention discloses a bacitracin derivative, a pharmaceutically acceptable salt thereof, a preparation method and application of the bacitracin derivative, a pharmaceutical composition and application of the pharmaceutical composition, and relates to the field of medicines. The invention discloses a bacitracin derivative and a pharmaceutically acceptable salt thereof, the structural general formula of the bacitracin derivative is as follows: the bacitracin derivative and the pharmaceutically acceptable salt thereof provided by the invention can be used for treating and / or preventing diseases or virus infectious diseases caused by bacterial infection.

The invention discloses a preparation method of methylene disalicylic acid, relates to the technical field of preparation of methylene disalicylic acid, and has the advantages that the methylene disalicylic acid prepared by the preparation method is high in conversion rate, a final product is low in polymer content and relatively high in chromatographic purity, and the product can be used as an intermediate for medicines. Similar derivatives are produced by adjusting the process for synthesis of bacitracin or other chemical materials; modularized production is flexible, the process is mature, skin and eye protection is paid attention to in the process, the preparation process of the method is high in safety, and solvent recycling and low-toxicity process design meet the green production requirement; according to the method, modular and standardized production can be adopted, the technological process is simplified, the equipment investment cost is reduced, low-toxicity solvents such as recyclable starch hydrolysate or corn fermentation products are used, environmental pollution is reduced, and the preparation quality of the method is improved.

The application discloses a kind of pharmaceutical compositions. The pharmaceutical composition includes A agent and B agent;The A agent includes Vibrio parahaemolyticus bacteriophage, Vibrio harveyi bacteriophage, Vibrio alginolyticus bacteriophage and Bacteriophage of Achromobacter nematophilus;The B agent includes bacitracin, short bacitracin, polymyxin and nisin. The present application can effectively inhibit and solve the "red bottom" phenomenon that appears in the bottom of pool in the process of breeding of snail, improve the success rate of breeding of snail.

The invention discloses a pharmaceutical composition. The pharmaceutical composition comprises an agent A and an agent B, the agent A comprises vibrio parahaemolyticus bacteriophage, vibrio harveyi bacteriophage, vibrio alginolyticus bacteriophage and bdellovibrio bacteriovorus; the agent B is prepared from bacitracin, brevibacin, polymyxin and nisin. The method can effectively restrain and solve the red bottom phenomenon of the pond bottom in the babylonia breeding process, and the babylonia breeding success rate is increased.

The invention relates to the technical field of food packaging materials, and discloses an allicin-canna edulis ker water extract-bacitracin D composite film for storage and preservation of sugar oranges, which is prepared by hydrolyzing fresh garlic, extracting by an ultrasonic-assisted deep-eutectic solvent method, and concentrating to obtain allicin. And then compounding allicin and bacitracin D with the canna edulis ker water extract according to a proper proportion, uniformly mixing, carrying out ultrasonic defoaming, and carrying out constant-temperature drying to prepare the composite membrane. The garlicin, the canna edulis ker water extract and the bacitracin D are integrated at the same time, the antioxidant activity can be effectively improved, then the antibacterial rate is increased, the prepared garlicin-canna edulis ker water extract-bacitracin D composite film is excellent in performance, and the preservative and fresh-keeping effects of the sugar oranges can be effectively improved.

The application discloses a bacitracin monoclonal antibody and application thereof, and belongs to the technical field of biology. The monoclonal antibody contains a heavy chain variable region and a light chain variable region, the heavy chain variable region and the light chain variable region are both composed of a complementarity determining region and a framework region, and the complementarity determining region is composed of CDR1, CDR2 and CDR3. The monoclonal antibody can be used for preparing a bacitracin detection preparation. The bacitracin colloidal gold detection test strip provided by the application has the characteristics of high sensitivity, good specificity and strong stability.

This invention belongs to the fields of genetic engineering and microbial technology, and discloses the application of the glucose dehydrogenase-encoding gdh gene in controlling the yield of secondary metabolites in Bacillus licheniformis. This invention involves knocking out the glucose dehydrogenase gene in Bacillus licheniformis. gdh ,get gdh Missing Bacillus licheniformis engineered strain DW2△ gdh and DW2-PR55-pgsB△ gdh Among them, DW2△ gdh The bacitracin production in different culture media was at least 7.5% higher than that of the control strain DW2, with a maximum increase of 10.6%. (DW2-PR55-pgsB△) gdh The production of polyγ-glutamic acid in different culture media was at least 28.75% higher than that of the control strain DW2-PR55-pgsB, and reached a maximum of 38.80%, indicating that this genetic engineering method can improve the production of secondary metabolites synthesized by microorganisms.

The invention discloses a preparation method of a bacitracin EP impurity F. The preparation method comprises the following steps: (1) synthesizing to obtain peptide chain body resin Reisn-Asn (R1)-D-Asp (R2)-His (R3)-D-Phe-Ile-D-Orn (R4)-Lys (R5)-Ile-D-Glu (R6)-Lu-R; (2) removing chlorine resin by using a cracking reagent; (3) removing a Lys side chain protecting group R5; (4) carrying out amide condensation reaction on an alpha-position carboxyl group of asparagine and an epsilon-position amino group of lysine by using a condensing agent to generate an intramolecular amide ring; and (5) removing other protecting groups, and purifying to obtain the target product bacitracin EP impurity F. A brand new synthesis route is designed to synthesize the bacitracin EP impurity F, and the bacitracin EP impurity F can be used as an impurity standard substance in the raw material medicine production process for comparison and plays an important role in controlling the impurity content in the raw material medicine production process.

The invention relates to the technical field of food packaging materials, and discloses a gordon euryale seed shell lignin-Chinese yam aqueous extract-bacitracin D composite antibacterial film as well as a preparation method and application thereof, gordon euryale seed shell lignin and bacitracin D are taken as base materials and integrated into a Chinese yam aqueous extract to prepare the composite antibacterial film, and effective preservation and fresh keeping of kumquats are realized. The composite antibacterial film is simple in preparation process, the prepared antibacterial film is excellent in performance, and the shelf life of kumquats can be effectively prolonged.

The invention discloses a bacitracin monoclonal antibody and application thereof, and belongs to the technical field of biology. The monoclonal antibody contains a heavy chain variable region and a light chain variable region, the heavy chain variable region and the light chain variable region are both composed of complementary determining regions and frame regions, and the complementary determining regions are both composed of CDR1, CDR2 and CDR3. The monoclonal antibody can be used for preparing a bacitracin detection product. The bacitracin colloidal gold test strip provided by the invention has the characteristics of high sensitivity, good specificity and strong stability.

The present invention provides a bacitracin-producing engineered bacterium with a knocked-out kipR gene, a preparation method and an application thereof, and uses a genetic engineering method to knock out the kipR gene in the genome of Bacillus licheniformis. kipR Gene, knocked out kipR The recombinant strain after the gene is the Bacitracin-producing engineered bacteria, kipR The nucleotide sequence of the gene is shown in SEQ ID NO. 1. The present invention successfully knocks out the gene encoding the transcriptional regulatory factor KipR in the genome of Bacillus licheniformis by genetic engineering methods. kipR Compared with Bacillus licheniformis DW2, the recombinant strain DW2△ kipR The bacitracin production was increased by more than 16%. The research results of the present invention show that: kipR Gene expression is a very effective method to increase the production of bacitracin.

This invention discloses an extraction solvent for bacitracin preparations in feed. It belongs to the field of product testing and analysis technology. The extraction solvent of this invention is a 12-15% ammonia-methanol-acetonitrile (1:1:1) solution, which can be used for extraction and analysis of bacitracin zinc and methylene salicylate bacitracin in different feeds using the same detection method, improving analytical efficiency, simplifying and increasing efficiency, and saving production costs. This invention also provides a detection method for bacitracin preparations in feed, using 12-15% ammonia-methanol-acetonitrile (1:1:1) as the extraction reagent. This not only optimizes the extraction conditions but also the purification conditions, greatly improving the accuracy and precision of the detection method of this invention, and providing technical support for the standardized use of bacitracin preparations in feed.

The present invention discloses the application of streptozotocin for antibacterial and combined antibacterial activity. The present invention discloses that streptozotocin has broad-spectrum antibacterial activity against both drug-resistant Gram-positive and Gram-negative bacteria. Furthermore, when used in combination with other antibacterial drugs, streptozotocin exhibits additive or synergistic antibacterial activity against drug-resistant bacteria. This can address the current shortage of clinical anti-resistant bacteria drugs, improve efficacy, and reduce the spread of drug-resistant bacteria.

This invention provides a nanobody that simultaneously binds bacitracin A and bacitracin B and its applications, belonging to the field of food and feed analysis and detection technology. The nanobody specifically binds to bacitracin A and bacitracin B. The VHH chain of the nanobody includes a complementarity-determining region (CDR) and a frame region, with the CDR separated by the frame region. The CDR includes CDR1, CDR2, and CDR3; the frame region includes FR1, FR2, FR3, and FR4. This nanobody can simultaneously target two bacitracins and exhibits excellent resistance to organic solvents and acids / alkalis. During sample pretreatment for actual detection, it is less affected by organic solvents and can also be used for detection in acidic and alkaline environments, providing a new solution for the detection of bacitracin-related substances.

The invention discloses an engineering strain for directional synthesis of bacitracin L as well as a recombinant vector and application of the engineering strain, and relates to the technical field of microbiology and biology. The engineering strain is bacillus amyloliquefaciens M86 into which the recombinant vector pCN33-PbmyD-sfGFP-LacI-YhiH is introduced; the recombinant vector pCN33-PbmyD-sfGFP-LacI-YhiH comprises a bmyD promoter, an enhanced green fluorescent protein sfGFP gene, a lactose operon regulation and control element LacI-MCS and an encoding gene of a transcription factor YhiH, and the encoding gene of the recombinant vector pCN33-PbmyD-sfGFP-LacI-YhiH comprises a bmyD promoter, an enhanced green fluorescent protein sfGFP gene, a lactose operon regulation and control element Expression of the transcription factor YhiH is regulated and controlled by a lactose operon, and the transcription factor YhiH can be specifically combined with a bmyD promoter, so that transcription of a bmyDABC gene cluster is activated, synthesis of bacitracin L is promoted, and directional synthesis of bacitracin L can be realized.

The invention relates to a cosmetic composition for the care of hair and scalp, comprising a first active ingredient selected from the group consisting of bacitracin, amyloslysine, plantazolicin, bacillomycin D, lactosporin, thuricin CD, bacillaene, and mixtures thereof; and a cell culture extract from Bacillus subtilis. The invention further relates to the use of this composition for the care of hair and scalp and for preventing dandruff on the scalp.

The present invention discloses an antibacterial coating and its application, relating to the technical field of surface modification of dental implants, in particular to an antibacterial coating. The preparation method of the antibacterial coating is as follows: in a Tris solution, under normal temperature and aerobic conditions, a mixture of dissolved catecholamine, bacitracin, and metal ions is reacted on a titanium sheet for 24 hours, and then the surface precipitate is ultrasonically cleaned and dried to obtain the antibacterial coating. The antibacterial coating has good antibacterial properties against Staphylococcus aureus, and the bacteriostatic rate reaches 72%. At the same time, the antibacterial coating is non-toxic to the human body and has good biocompatibility.

The application provides a bacitracin engineering bacterium with an knocked-out amtR gene, a preparation method and application thereof, wherein the bacitracin engineering bacterium is obtained by knocking out the amtR gene in the genome of Bacillus licheniformis by genetic engineering means, and the nucleotide sequence of the amtR gene is shown in SEQ ID NO. 1. The application provides a new strategy for improving the yield of bacitracin. Compared with Bacillus licheniformis DW2, the yield of bacitracin of the recombinant strain DW2△amtR of Bacillus licheniformis constructed by the application is increased by more than 18 %.

The invention relates to a bacitracin fluorescence response system based on a two-component system and application of the bacitracin fluorescence response system, and belongs to the technical field of bioengineering. According to the bacitracin fluorescent response system constructed by the invention, bceR and bceS proteins are expressed by using a promoter PbceR, fluorescent protein is expressed by using a promoter PbceA, a signal amplification device comprising T7RNA polymerase and a T7 promoter is added to the upstream of the fluorescent protein, then the structure of the fluorescent protein is optimized, and the fluorescent protein is obtained by replacing an RBS sequence and truncating the promoter PbceA. The fluorescence response signal is further improved. The fluorescent response system is integrated and expressed in bacillus subtilis to obtain the bacitracin whole-cell biosensor, and the bacitracin whole-cell biosensor can be applied to high-throughput screening of bacitracin high-yield strains and provides a brand-new detection tool for efficient production of bacitracin.