20 results about "Bacitracin" patented technology

The application provides application of bacitracin or a composition of the bacitracin and oregano essential oil in improving meat quality of ruminants, and relates to the technical field of animal feed. The bacitracin is used as an active ingredient for preparing feed, and the addition amount of the bacitracin in the feed is 1.0-2.4 g / kg. The active ingredient also comprises oregano essential oil, and the mass ratio of the bacitracin to the oregano essential oil is 3.5-4.0:1. The bacitracin can be used for improving meat quality of ruminants for the first time, and when the bacitracin is used in combination with the oregano essential oil, synergistic effects can be produced in meat color, tenderness, fatty acid composition and formation of volatile flavor substances, muscle quality of different parts is improved, meat quality is effectively improved, the comprehensive commodity value of ruminant meat is greatly improved, and the bacitracin has a good popularization prospect.

The present application provides a bacitracin-producing engineering bacterium with oxdC gene knocked out and a preparation method and application thereof, which adopts a genetic engineering means to knock out an oxdC gene in a genome of Bacillus licheniformis, and obtains a recombinant strain after the oxdC gene is knocked out, namely the bacitracin-producing engineering bacterium, and the nucleotide sequence of the oxdC gene is shown in SEQ ID NO. 1. The bacitracin-producing engineering bacterium has stronger fermentation production capacity and higher yield, and the bacitracin yield is increased by more than 16% compared with Bacillus licheniformis DW2.

The invention provides Kineococcus G2 and application of the Kineococcus G2 in preparation of a pigment with antioxidant activity, the Kineococcus new strain G2 is CCTCC No. M20242602, the strain is spherical, the size is 1.0 * 1.5 mu m, the optimal growth temperature is 30-37 DEG C, the pH value is 7.0-9.0, and the salt concentration is 1.0%-2.0%. The strain G2 is not sensitive to bacitracin, ciprofloxacin and norfloxacin, and is sensitive to novobiocin, polymyxin, gentamicin, piperacillin, ofloxacin, erythromycin, streptomycin, kanamycin, rifampicin, ampicillin, vancomycin, carbenicillin, chloramphenicol, tetracycline, penicillin, cefoperazone, oxacillin, amoxicillin and neomycin. Pigment generated by fermentation of the G2 strain has good antioxidant activity, and when the concentration is 10 micrograms / mL, the scavenging activity on DPPH is 25.00%, the scavenging activity on hydroxyl radicals is 80.58%, and the iron ion reducing capacity is 1.57, which are all higher than those of contrast beta-carotenoid. The application of the new Kineococcus strain G2 can lay a foundation for the research of antioxidant drugs such as medical treatment and health care product development.

This invention relates to microbial culture. It provides a *Bacillus parabregas* culture medium and its applications, wherein the medium comprises at least one of glycerol, yeast extract, soybean peptone, corn steep liquor powder, ammonium sulfate, magnesium sulfate, and calcium carbonate. The *Bacillus parabregas* culture medium of this invention utilizes glycerol as a carbon source and yeast extract, soybean peptone, etc., as nitrogen sources for large-scale liquid fermentation of *Bacillus parabregas*, achieving a total bacitracin content of over 10.5 g / L. The proportions of each component can be controlled by feeding. Using this medium for *Bacillus parabregas* fermentation results in high bacitracin yield and controllable composition. The medium formulation is simple, inexpensive, readily available, safe, and environmentally friendly. The fermentation process is simple and controllable, with a short fermentation cycle, facilitating industrial-scale production and possessing high application value.

The invention discloses a purification method and application of bacitracin A, and relates to the field of biology. According to the method for purifying the bacitracin A, preparation of the high-purity bacitracin A is achieved through liquid chromatographic separation, the purity of the bacitracin A can reach 95% or above, the separation and purification process of the high-purity bacitracin A is simplified to a great extent, the loss of reagents in the separation and purification stage is reduced, and the yield of the high-purity bacitracin A is increased. The method has the advantages of high separation purity, less organic solvent dosage, simple device, simple and convenient process operation, environmental protection and the like, and is suitable for process production.

The application discloses a kind of pharmaceutical compositions. The pharmaceutical composition includes A agent and B agent;The A agent includes Vibrio parahaemolyticus bacteriophage, Vibrio harveyi bacteriophage, Vibrio alginolyticus bacteriophage and Bacteriophage of Achromobacter nematophilus;The B agent includes bacitracin, short bacitracin, polymyxin and nisin. The present application can effectively inhibit and solve the "red bottom" phenomenon that appears in the bottom of pool in the process of breeding of snail, improve the success rate of breeding of snail.

The invention relates to the technical field of food packaging materials, and discloses an allicin-canna edulis ker water extract-bacitracin D composite film for storage and preservation of sugar oranges, which is prepared by hydrolyzing fresh garlic, extracting by an ultrasonic-assisted deep-eutectic solvent method, and concentrating to obtain allicin. And then compounding allicin and bacitracin D with the canna edulis ker water extract according to a proper proportion, uniformly mixing, carrying out ultrasonic defoaming, and carrying out constant-temperature drying to prepare the composite membrane. The garlicin, the canna edulis ker water extract and the bacitracin D are integrated at the same time, the antioxidant activity can be effectively improved, then the antibacterial rate is increased, the prepared garlicin-canna edulis ker water extract-bacitracin D composite film is excellent in performance, and the preservative and fresh-keeping effects of the sugar oranges can be effectively improved.

The application discloses a bacitracin monoclonal antibody and application thereof, and belongs to the technical field of biology. The monoclonal antibody contains a heavy chain variable region and a light chain variable region, the heavy chain variable region and the light chain variable region are both composed of a complementarity determining region and a framework region, and the complementarity determining region is composed of CDR1, CDR2 and CDR3. The monoclonal antibody can be used for preparing a bacitracin detection preparation. The bacitracin colloidal gold detection test strip provided by the application has the characteristics of high sensitivity, good specificity and strong stability.

This invention belongs to the fields of genetic engineering and microbial technology, and discloses the application of the glucose dehydrogenase-encoding gdh gene in controlling the yield of secondary metabolites in Bacillus licheniformis. This invention involves knocking out the glucose dehydrogenase gene in Bacillus licheniformis. gdh ,get gdh Missing Bacillus licheniformis engineered strain DW2△ gdh and DW2-PR55-pgsB△ gdh Among them, DW2△ gdh The bacitracin production in different culture media was at least 7.5% higher than that of the control strain DW2, with a maximum increase of 10.6%. (DW2-PR55-pgsB△) gdh The production of polyγ-glutamic acid in different culture media was at least 28.75% higher than that of the control strain DW2-PR55-pgsB, and reached a maximum of 38.80%, indicating that this genetic engineering method can improve the production of secondary metabolites synthesized by microorganisms.

The invention relates to the technical field of food packaging materials, and discloses a gordon euryale seed shell lignin-Chinese yam aqueous extract-bacitracin D composite antibacterial film as well as a preparation method and application thereof, gordon euryale seed shell lignin and bacitracin D are taken as base materials and integrated into a Chinese yam aqueous extract to prepare the composite antibacterial film, and effective preservation and fresh keeping of kumquats are realized. The composite antibacterial film is simple in preparation process, the prepared antibacterial film is excellent in performance, and the shelf life of kumquats can be effectively prolonged.

The invention discloses a bacitracin monoclonal antibody and application thereof, and belongs to the technical field of biology. The monoclonal antibody contains a heavy chain variable region and a light chain variable region, the heavy chain variable region and the light chain variable region are both composed of complementary determining regions and frame regions, and the complementary determining regions are both composed of CDR1, CDR2 and CDR3. The monoclonal antibody can be used for preparing a bacitracin detection product. The bacitracin colloidal gold test strip provided by the invention has the characteristics of high sensitivity, good specificity and strong stability.

This invention discloses an extraction solvent for bacitracin preparations in feed. It belongs to the field of product testing and analysis technology. The extraction solvent of this invention is a 12-15% ammonia-methanol-acetonitrile (1:1:1) solution, which can be used for extraction and analysis of bacitracin zinc and methylene salicylate bacitracin in different feeds using the same detection method, improving analytical efficiency, simplifying and increasing efficiency, and saving production costs. This invention also provides a detection method for bacitracin preparations in feed, using 12-15% ammonia-methanol-acetonitrile (1:1:1) as the extraction reagent. This not only optimizes the extraction conditions but also the purification conditions, greatly improving the accuracy and precision of the detection method of this invention, and providing technical support for the standardized use of bacitracin preparations in feed.

This invention provides a nanobody that simultaneously binds bacitracin A and bacitracin B and its applications, belonging to the field of food and feed analysis and detection technology. The nanobody specifically binds to bacitracin A and bacitracin B. The VHH chain of the nanobody includes a complementarity-determining region (CDR) and a frame region, with the CDR separated by the frame region. The CDR includes CDR1, CDR2, and CDR3; the frame region includes FR1, FR2, FR3, and FR4. This nanobody can simultaneously target two bacitracins and exhibits excellent resistance to organic solvents and acids / alkalis. During sample pretreatment for actual detection, it is less affected by organic solvents and can also be used for detection in acidic and alkaline environments, providing a new solution for the detection of bacitracin-related substances.

The invention discloses an engineering strain for directional synthesis of bacitracin L as well as a recombinant vector and application of the engineering strain, and relates to the technical field of microbiology and biology. The engineering strain is bacillus amyloliquefaciens M86 into which the recombinant vector pCN33-PbmyD-sfGFP-LacI-YhiH is introduced; the recombinant vector pCN33-PbmyD-sfGFP-LacI-YhiH comprises a bmyD promoter, an enhanced green fluorescent protein sfGFP gene, a lactose operon regulation and control element LacI-MCS and an encoding gene of a transcription factor YhiH, and the encoding gene of the recombinant vector pCN33-PbmyD-sfGFP-LacI-YhiH comprises a bmyD promoter, an enhanced green fluorescent protein sfGFP gene, a lactose operon regulation and control element Expression of the transcription factor YhiH is regulated and controlled by a lactose operon, and the transcription factor YhiH can be specifically combined with a bmyD promoter, so that transcription of a bmyDABC gene cluster is activated, synthesis of bacitracin L is promoted, and directional synthesis of bacitracin L can be realized.

The invention relates to a cosmetic composition for the care of hair and scalp, comprising a first active ingredient selected from the group consisting of bacitracin, amyloslysine, plantazolicin, bacillomycin D, lactosporin, thuricin CD, bacillaene, and mixtures thereof; and a cell culture extract from Bacillus subtilis. The invention further relates to the use of this composition for the care of hair and scalp and for preventing dandruff on the scalp.

The application provides a bacitracin engineering bacterium with an knocked-out amtR gene, a preparation method and application thereof, wherein the bacitracin engineering bacterium is obtained by knocking out the amtR gene in the genome of Bacillus licheniformis by genetic engineering means, and the nucleotide sequence of the amtR gene is shown in SEQ ID NO. 1. The application provides a new strategy for improving the yield of bacitracin. Compared with Bacillus licheniformis DW2, the yield of bacitracin of the recombinant strain DW2△amtR of Bacillus licheniformis constructed by the application is increased by more than 18 %.

The invention relates to a bacitracin fluorescence response system based on a two-component system and application of the bacitracin fluorescence response system, and belongs to the technical field of bioengineering. According to the bacitracin fluorescent response system constructed by the invention, bceR and bceS proteins are expressed by using a promoter PbceR, fluorescent protein is expressed by using a promoter PbceA, a signal amplification device comprising T7RNA polymerase and a T7 promoter is added to the upstream of the fluorescent protein, then the structure of the fluorescent protein is optimized, and the fluorescent protein is obtained by replacing an RBS sequence and truncating the promoter PbceA. The fluorescence response signal is further improved. The fluorescent response system is integrated and expressed in bacillus subtilis to obtain the bacitracin whole-cell biosensor, and the bacitracin whole-cell biosensor can be applied to high-throughput screening of bacitracin high-yield strains and provides a brand-new detection tool for efficient production of bacitracin.

The invention belongs to the field of genetic engineering and microbial engineering, and particularly relates to a mannitol transporter MtlA mutant derived from bacillus licheniformis and application of the mannitol transporter MtlA mutant in production of metabolites. By adopting a site-specific mutagenesis method, valine at the 325th site of the mannitol transporter MtlA from bacillus licheniformis is mutated into isoleucine, so that the activity of the mannitol transporter is remarkably improved, and the problem that the mannitol transporter is relatively low in mannitol transport performance at present is effectively solved; the strain is successfully applied to fermentation production of bacitracin and hydroxytyrosol, and a new thought is provided for improving the production level of metabolites.

The invention discloses a method of a Xinjiang early yellow fig tissue culture and rapid propagation system, and belongs to the technical field of plant tissue culture. The method comprises the steps of explant selection and treatment, induction culture, subculture, rooting culture and domestication and transplanting. In the explant pretreatment, a bacitracin-ursolic acid-abamectin conjugate is used for disinfection, so that the bacitracin and abamectin cooperate to resist bacteria and prevent microbial infection, and ursolic acid is used for inhibiting the activity of polyphenol oxidase to prevent explant browning; hormone-enriched microspheres with chitosan as a core layer and calcium alginate-lignosulfonate as a shell layer are added into the culture medium, BA or NAA can be stably stored and accurately released, nutrient substances can also be adsorbed, meanwhile, mannitol, L-proline and betaine loaded on the microspheres can adjust osmotic pressure, enhance cell stress resistance and reduce vitrification, and the hormone-enriched culture medium is suitable for being used as a culture medium. The rapid propagation of the fig explant can be efficiently promoted, and a feasible technical path is provided for the tissue propagation of the fig explant.

The invention relates to bacillus sooraensis Y2 with high bacitracin yield and application of the bacillus sooraensis Y2, and belongs to the technical field of fermentation. According to the invention, a new strain of bacillus sorola desert bacillus Y2 is obtained through screening, and the bacillus sorola desert bacillus Y2 disclosed by the invention is capable of resisting high-concentration bacitracin while being capable of producing the bacitracin at high yield. Through mutagenesis and screening, 3.57 g / L of bacitracin can be produced under the optimal fermentation condition by the bacillus sorola desert bacillus Y2, and the yield of the bacitracin is far higher than that of the bacitracin of the existing bacillus sorola desert bacillus. In addition, the bacillus sorola disclosed by the invention can also tolerate bacitracin with high concentration, so that the bacillus sorola Y2 can still maintain the growth state along with the increase of the concentration of the bacitracin in fermentation liquor, and the toxic action on zymophyte due to the increase of the concentration of the bacitracin is avoided.