146 results about "Bdellovibrio" patented technology

The invention relates to fermentation culturing technology of high density Bdellovibrio telotroch, comprising the steps as following: firstly, culturing the host-germ shaking table and adding DNB liquid after centrifugating to obtain host-germ suspension; secondly; adding the host-germ suspension into the DNB liquid and then adding the Bdellovibrio to culture, at last adding DNB liquid after centrifugating to obtain Bdellovibrio concentrated solution; thirdly; adding sodium glutamate into DNB liquid and then adding the host-germ suspension, at last adding the Bdellovibrio concentrated solution to make the concentration 1 to 10<3>PFU/mL. The fermentation liquid of the Bdellovibrio with density of 10<8> to 10<14>PFU/mL can be obtained by culturing in a fermentation cylinder for four to six days and the concentrated solution of Bdellovibrio telotroch can be obtained through concentration by centrifuging. The invention has the advantages of low cost, high final concentration of Bdellovibrio and wide application range, and can be used not only in the control of water source pathogen and food pathogen, but also in fields of medical science, environmental pollution, agriculture, industry and military matters.

The invention discloses an application of bdellovibrios in removing pathogenic vibrio in marine food products and the culture water. The bdellovibrio concentrated solution is added into the marine food products and/or the culture water so as to lead the concentration of the bdellovibrio to reach at least 10<2>pfu /ml. When the invention is applied before eating the marine food products, in the transportation process and in the culture water, the concentration range of bdellovibrio is respectively 10<4>-10<12> pfu /ml, 10<3>-10<11> pfu /ml, and 10<2>-10<6 > pfu /ml. More than 90% pathogenic vibrio carried by marine food products before eating and/or in the transportation process is cleared by adopting a biological method, and the pathogenic vibrio in the culture water can also be controlled within10cfu/ml. The invention is suitable for the pretreatment process before being eaten or processed, the transportation process and the culture process of marine food products, especially facilitating the reduction or elimination of chemical medicine residues such as antibiotics, thus fundamentally avoiding the occurrence of food poisoning of the marine food products.

The invention discloses a Bdellovibrio strain for preventing and treating mastitis and applications thereof. A Bdellovibrio BDS01 is obtained by separation, and is unicellular, arced and 0.9-0.25 micrometer in size; the end of the Bdellovibrio BDS01 has flagellum which is 4 micrometers long. When cultured by a two-layer plating method at 28 DEG C for four days, the Bdellovibrio BDS01 can form transparent round plaques with the diameter of 1-2 mm. The microbial preparation prepared by using the Bdellovibrio BDS01 plastid has favorable effects for preventing cow mastitis. Compared with the telotroch, the plastid has the characteristics of convenient preparation and storage, strong environmental resistance, slightly slow sterilization action, longer sterilization action time, and the like, and does not have toxic or side effect on cows. Therefore, the microbial preparation containing the Bdellovibrio BDS01 plastid is suitable for large-size factory culture of cows, and a novel method forgreen processing and production of milk and for preventing and treating mastitis can be provided.

The invention relates to a feedstuff used for culturing fresh-water crabs. The composition of the feedstuff comprises import fishmeal powder, soybean meal, peanut meal, cotton meal, rapeseed meal, marine yeast, citric acid dregs, flour, soybean phospholipids, cuttle fish grease, bentonitic clay and primary calcium phosphate, and also comprises crabs-using multivitamin, crabs-using multiminerals, compound bacillus subtilis, microzyme, bdellovibrio, biological antibacterial peptide and microcapsule kitasamycin; Additive formulation mainly provides vitamin, mineral ion element, various enzymes, etc., for accelerating the growth of the fresh-water crabs. In addition, the additive formulation do not do harm to the growth of the fresh-water crabs. The additive also comprise antibiotics and various enzymes used for controlling the growth of hazardous enzymes in internal environment of the fresh-water crabs; therefore, after eating the feedstuff containing basic ingredients and the ingredients of the additive, the fresh-water crabs has remarkable growth effect without any side effects.

The invention relates to a penaeus vannamei boone ecological culturing system, which comprises a high level intensive culturing area, a circulating water passage, a shellfish culturing area, a salt-resistant plant area and a water quality treatment area, wherein the high level intensive culturing area, the shellfish culturing area, the salt-resistant plant area and the water quality treatment area are communicated by the circulating water passage. Before the penaeus vannamei boone is put into the system, bdellovibrio bacteria liquid and nitrifying bacteria are added into water of the high level intensive culturing area. During culturing, the traditional Chinese medicine liquid is added into the high level intensive culturing area. The system has the advantages that a culturing water body can be recycled, so the drainage of sewage is reduced, and the pressure on the ecological environment is reduced; the body immunity of the penaeus vannamei boone is improved, and the possibility of the disease outbreak and prevalence of the penaeus vannamei boone is reduced; the shellfishes, aquatic plants, mangroves and seaweeds are put into the system, so the economic benefit of culturing is improved; the whole culturing process realizes the culturing production without using antibiotics, the green and environment-friendly effects of the produced penaeus vannamei boone are realized, the mouth feel is similar to the taste of wild shrimps, and the penaeus vannamei boone is popular in market.

The invention discloses a microbial preparation, an aquatic feed and an aquaculture method and relates to the technical field of aquaculture. The microbial preparation comprises bdellovibrio and probiotic. Optimally, the probiotic is selected from any one of lactobacillus bulgaricus, lactobacillus delbrueckii, enterococcus faecalis, lactobacillus casei, lactobacillus acidophilus, rhodopseudomonaspalustris, clostridium butyricum, bacillus laterosporus, bacillus pumilus, bacillus coagulans, bacillus licheniformis, bacillus subtilis and lactobacillus plantarum. The microbial preparation has thecharacteristics of capability of increasing food intake of aquaculture animals, capability of promoting growth rate, survival rate and immunity of organisms, and the like. Besides, the microbial preparation is capable of effectively preventing and treating the problems of diseases caused by pathogenic bacteria, such as vibrio parahaemolyticus, vibrio harveyi, luminous vibrio, vibrio alginolyticus,vibrio campbellii, vibrio fluvialis, vibrio vulnificus, aeromonas, vibrio cholerae, pseudomonas and salmonella.

The invention relates to a method and treatment agent for preventing and controlling blue-green algae. A cyanophage bacterium S agent is a culture solution with the solid content of 0.5-2 percent, which is prepared from spirulina powder, NaCl and purified water; a biological substrate modified agent of K agent is prepared by compounding saccharomycetes, bacillus subtilis and cyanophage bdellovibro; and an algal removing water purifying agent of L agent is prepared from 40wt% of sodium carbonate, 50wt% of fourth generation of polyamidoamine and 10 wt% of sodium bentonite. The invention overcomes the defects of two methods by using beneficial bacterium which scrambles nutrition with the blue-green algae and cyanophage capable of phagocytosing the blue-green algae. The invention is a system engineering for preventing and controlling blue-green algae, which organically combines a biological method and a chemical method and can prevent and control blue-green algae comprehensively with proper cost and favorable practicability and stability; and the blue-green algae is controlled by using a microbial method at early stage with obvious implementation effect , since a cell wall of the blue-green algae is thinner and cyanophage can phagocytose algae quickly; in addition, since a water body has high eutrophication degree in summer, comprehensive treatment can achieve good effect.

The invention discloses a bactericide for preventing and curing crop bacterial diseasess as well as a preparation method and application thereof. In the preparation method, oligosaccharide is added into bdellovibrio bacteriovorus solution to be evenly mixed to obtain the bactericide for preventing and curing crop bacterial diseasess. The invention applies bdellovibrio bacteriovorus solution for preventing and curing crop bacterial diseases for the first time, fills the vacancy of researching and developing bdellovibrio bacteriovorus at home and abroad and adds new species for biopesticide. Asbdellovibrio bacteriovorus has the characteristics of broad cracking pattern and quick growth and has the outstanding capability of eliminating various pathogenicbacteria and bacteria, after combinedwith oligosaccharide, the bdellovibrio bacteriovorus strengthens crop resistance, greatly improves pesticide effect and enlarges the preventing and curing spectrum of bacterial diseases. The bactericide prepared by the invention has no toxicity or pollution, is safe to natural enemies of crop diseases and pests, pathogenic bacteria have small possibility of producing resistance, so that the invention overcomes 3R problems brought by abusing antibiotics and avoids the defects of serious chemical pesticide pollution and ecological balance damage at present.

The invention relates to active biological antibacterial and a production method thereof. The active biological antibacterial is prepared by proportionally mixing the cultures of bdellovibrio and bacteriophage serving as the main ingredients in the following steps: obtaining the bdellovibrio culture and the bacteriophage culture respectively, and preparing the cultures at a proportion of 1:1-1:1,000,000, namely that the bdellovibrio content is 10-1,010/pfu, and the bacteriophage content is 10-1,016/pfu; and mixing the cultures so that the cultures are suspended in medium liquid or mixed in excipient. The invention is used for preventing and controlling the bacterial diseases for human body, animals and plants, and has obvious effects on guaranteeing quality and freshness of fruits, vegetables, flowers, food and aquatic products, eliminating harmful bacteria in the environment and suppressing growth of blue-green algae.

The invention discloses an environment-friendly biological pesticide which is characterized by comprising the following components in parts by weight: 0.001-0.002 parts of shigella dysenteriae bacillus aceticus powder, 0.001-0.002 parts of bdellovibrio powder, 0.001-0.002 parts of phage powder, 0.001-0.002 parts of oceanospirillales powder, 20-26 parts of tea seed cake, 10-16 parts of rape seed cake, 1-10 parts of synergist, 5-20 parts of taraxacum, 1-3 parts of rhizoma smilacis glabrae, 1-3 parts of ground beeltle, 1-3 parts of indian adenosma, 1-3 parts of pericarpium arecae, 1-3 parts of screwtree roots, 12-18 parts of houttuynia cordata, 5-18 parts of common andrographis herb and 100-150 parts of water. The environment-friendly pesticide is a novel pesticide which is efficient, targeted, environment-friendly and nuisanceless, has no secondary toxicity, residue or pollution, and keeps the ecological balance, is an important guarantee for achieving ecological agriculture, green ecological agriculture and a green enterprise, and has a wide prospect.

The invention relates to a biological wall-breaking method for improving sludge dewatering performance, and belongs to the technical field of sludge treatment. According to the method, the phagocytosis characteristic that bdellovibrio can widely and effectively decompose Gram-negative bacteria is sufficiently utilized, bdellovibrio is combined with other microorganisms to prepare bdellovibrio complex microbial inoculants which are applied to the treatment process of excess sludge of the biological treatment process of a municipal wastewater treatment plant so that the dewatering performance of treated sludge can be improved by 30% or higher after subsequent plate frame press filtering or centrifugal dewatering or other physical treatment, and therefore the sludge reduction efficiency is improved. The method treats remaining sludge of the municipal wastewater biological treatment process, is little in device investment, easy to operate, safe, free of pollution and low in energy consumption, and has good economic benefits and environment benefits, and a new method for municipal sludge biological dewatering reduction treatment is developed.

The invention discloses a method for separating purified bdellovibrio from active sludge. By virtue of key techniques of host bacteria screening and active sludge pretreatment and technical steps such as preparation of a culture medium and a culture solution and optimization of culture conditions and the like, efficient bdellovibrio is separated and purified from a homologous sludge sample by optimization of a series of key steps by virtue of gram negative bacteria which are obtained in active sludge as host bacteria for separating and purifying bdellovibrio. The invention not only puts forwards a method for successfully separating the efficient bdellovibrio from the active sludge of a municipal wastewater treatment plant for the first time, but also the culture period of the method is remarkably shortened, so that the defects that the existing conventional method is unstable in bdellovibrio separating effect, long in culture period, tedious in operation and the like.

The invention discloses a bdellovibrio bacteriovorus freeze-dried powder preparation. The preparation is prepared by the following steps: culturing bdellovibrio bacteriovorus by adopting a plurality of mixed live host bacteria; performing filtration to remove host pathogenic bacteria so as to obtain a bdellovibrio bacteriovorus seed liquid; culturing the seed liquid by using a plurality of mixed inactivated host bacteria; adding a freeze-drying protective agent into the obtained bdellovibrio culture solution, and performing uniform mixing; adding a freeze-drying carrier, and performing mixingto obtain a preparation to be freeze-dried; and finally performing freeze-drying to obtain the preparation. The invention also discloses a preparation method and application of the preparation. The preparation disclosed by the invention has a lysis effect on various aquatic pathogenic bacteria, is efficient and safe, solves the problems of phage singleness and environmental pollution of the bacteriophage bdellovibrio generated by taking single active pathogenic bacteria as host bacteria in the prior art, ensures the phage diversity of the bacteriophage bdellovibrio, and improves the lysis activity of the bacteriophage bdellovibrio.

The invention provides a method for cultivating young portunid, comprising the following steps of early-stage preparation: soaking a cultivation pond with clean water for 1-2 days, then sterilizing the cultivation pond with 50ppm-100ppm bleaching powder, paving sterilized sea mud on the bottom of the cultivation pond, introducing sea water and adding bdellovibrio solution and nitrifying bacteria; spawning; after-treatment: throwing bacillus solution during the cultivation process. By the adoption of the method provided by the invention, the water can be purified, the water quality can be improved, and the residues caused by a conventional cultivation method can be prevented; meanwhile, as the water is not changed during the cultivation process, the growing cost is reduced; not only is the survival rate of the young portunid improved, but also the immunity of the young portunid can be improved; with the utilization of the method, the abuse of drugs such as antibiotics in production is avoided; the incidence of diseases is lowered; the production yield is improved.

The invention discloses a bdellovibrio bacteriovorus bacterial strain for eliminating listeria monocytogenes and an application thereof. The invention obtains bdellovibrio bacteriovorus BDSM08 by separation, purification and ultraviolet mutation. BDSM08 is in the arc-shaped unicellular form when observed by an electron microscope, the size thereof is 1.8*1.0 mu m, the ends thereof are provided with flagellum and the length of the flagellum is 3.2 mu m. The bdellovibrio bacteriovorus BDSM08 is cultivated with the two layer plating method at the temperature of 28 DEG C for three days to form transparent round negativecolony the diameter of which is 2-3mm and the optimum growing pH value of which is 7.2, the optimum growing temperature of which is 28 DEG C and the optimum salinity of which is0%. The microbial agent prepared by BDSM08 has a strong effect on killing listeria monocytogenes which can be commonly seen in environment. The invention applies the bdellovibrio bacteriovorus BDSM08for the first time to prevent and cure food listerellosis; the invention has a favourable effect on eliminating listeria monocytogenes on food, has characteristics of no toxicity, no side effect on food and the like, and has favourable application prospect.

The invention relates to a method for preparing a bdellovibrio preparation by an enrichment culture method, belongs to the field of biotechnology and relates to an enrichment culture method of variousbdellovibrio, host bacteria culture and production methods of biological preparations thereof. The method is characterized in that a bdellovibrio system naturally formed in aquaculture water culturedby the enrichment culture method is used as a source of the bdellovibrio of the biological preparation, and pathogenic vibrio in the aquaculture water is purified, cultured, centrifuged, inactivatedand then used as a source of the host bacteria for the production of the bdellovibrio preparation to produce the biological preparation containing various bdellovibrio. The bdellovibrio preparation produced by the method has the advantages of high activity, rapid action and broad cleavage of pathogenic bacteria in the water due to the various bdellovibrio.

The invention relates to a compound biological bacterial fertilizer. The compound biological bacterial fertilizer is prepared from the following raw materials: Bdellovibrio BDS01, Bdellovibrio BDS02, Nitrosomas, denitrifying bacteria, Streptococcus thermophilus, Saccharomyces cerevisiae, Lactobacillus bulgaricus, Leuconostoc, Pediococcus, Bacillus subtilis BSD-2, Actinomyces S317, Trichoderma T36, Bacillus spp B135, Saccharomyces E7, lactic acid bacteria L2, Bacillus coagulans, aerobic spore-bearing bacilli, anaerobic lactic acid bacillus, Rhodopseudomonas palustris, Pseudoalteromonas, asparagus bacteria, Firmicutes, Planctomycetes, cyanobacteria, Actinomyces, Bacillus natto, live bacteria of Clostridium butyricum, photosynthetic bacteria and butyric acid bacteria. The compound biological bacterial fertilizer provided by the invention is applicable as a plantation fertilizer and a biological feed and can protect the environment and human health.

The invention discloses a bdellovibrio disinfectant as well as a preparation method and application thereof and particularly discloses a phage bdellovibrio granula which belongs to the field of microbial immobilization. The method comprises the steps as follows: (1), preparation of bdellovibrio liquid: bdellovibrio strain is inoculated in colon bacillus suspension for enrichment culture so as to enable the concentration of the bdellovibrio liquid to be 1.0*109 CFU/mL to 1.0*1013 CFU/mL; and (2), preparation of bdellovibrio embedded granula: stirring the mixture of bdellovibrio liquid, agar colloidal solution, glutaraldehyde and peanut oil for 30 min at a constant temperature, then dripping the mixture in calcium chloride for immobilization, and freeze drying for 24 h to obtain bdellovibrio granula. By combination of the imbedding method and the glutaraldehyde crosslinking method, the invention prepares the phage bdellovibrio granula which has better vitality and mechanical strength, is economical and safe, can be stored for a long time, and can be applied to a plurality of industries like aquaculture and livestock and poultry raising.

The invention discloses a water-saving haliotis diversicolor aquatilis cultivation method which comprises the following steps: taking unpolluted deep sea water in the marine space as the cultivation water; selecting healthy and good-conditioned haliotis diversicolor aquatilis fingerlings and putting them into the cultivation pool; pouring bdellovibrio swimming body?bacterial liquid into the cultivation pool till the concentration of the bdellovibrio swimming body reaches 10-10<7> pfu/ml; feeding once a day with the amount of bait about 5% to 15% of the weight of the haliotis diversicolor aquatilis; soaking the bait in the bdellovibrio swimming body bacterial liquid with a concentration of 10-10<7> pfu/ml; and changing the pool water once every 5 to 30 days and sprayingm the bdellovibrio swimming body ?bacterial liquid after each water change. The cultivation method provided by this invention noticeably facilitates the growth, immunity and survival rate of haliotis diversicolor aquatilis and improves the water quality of the cultivation environment. Due to no toxic or side effect of the bdellovibrio swimming body bacterial liquid to abalone, the bdellovibrio swimming body bacterialliquid can be applied to large-scale industrialized abalone cultivation.

The invention discloses a deodorization method in treatment of printing and dyeing wastewater, which comprises the following steps of: treating the printing and dyeing wastewater by an adjusting pool, an acidification pool and an oxidation pool in sequence; and at the same time of treating the printing and dyeing wastewater, dripping composite microbial liquid in the front end of the adjusting pool and the front end of the acidification pool respectively, and dripping another composite microbial liquid in the front end of the oxidation pool, wherein viable bacteria in the composite microbial liquid at least comprise bacteriophagic Bdellovibrio, photosynthetic bacteria group, lactic acid bacteria group, yeast group, Gram-positive bacteria Actinomyces series, filamentous bacteria group of fermentation series; and the viable bacteria in the another composite microbial liquid at least comprise bacteriophagic Bdellovibrio, red yeast group, photosynthetic bacteria group, lactic acid bacteria group, yeast group, Gram-positive bacteria Actinomyces series, filamentous bacteria group of fermentation series. The deodorization method disclosed by the invention is used for deodorizing while the sewage is treated, so that the treatment cost is lowered, the sewage treatment time is shortened, the deodorization working efficiency is improved, and good treatment effect is achieved.

The invention discloses an energy-saving and emission-reducing scophthalmus maximus seedling culture method. The method comprises the following steps of: (1) selection of parent fish; (2) parent fish enrichment culture; (3) egg taking, fertilization and hatching; (4) distribution in a pond; (5) bait feeding; and (6) harvesting. By adopting the technical of combining prevention and adjustment of bdellovibrio nectophore and leech plasmids and environmental control, mixed bacteria solution of the bdellovibrio nectophore and the leech plasmids is added into a seedling culture pond, and the concentration of the mixed bacteria solution in the water body is 10 to 10<7> pfu/mL; meanwhile, the feed is soaked into the 10<7> pfu/mL mixed bacteria solution of the bdellovibrio nectophore and the leechplasmids, the average seedling culture survival rate reaches over 50 percent, the whitening rate is reduced to 3.4 percent, and the aberration rate is reduced to 2.3 percent; and the method can effectively control the bacteria number in the water body, improves the water quality, does not need to change water in 40 days, saves the cost, greatly improves the economic benefit, and is a green low-carbon novel pollution-free seedling culture method.

A bdellovibrio particulate agent is characterized by being prepared by: mixing plant oil, agar with a mass concentration of 5%-10%, and a bacteria liquid with a bdellovibrio content of 10<10>-10<11> CFU/mL according to a volume ratio of 3:50:100, then adding sodium carboxymethylcellulose with a mass concentration of 2% and mixing uniformly, dropwise adding the mixed solution into a CaCl2 solution with a mass percentage of 10%, solidifying for 12 h, and then performing freeze drying at -20 DEG C for 24 h on bdellovibrio solidified particles to obtain the particulate agent. The bdellovibrio particulate agent is high in mechanical strength, the bdellovibrio activity is well stored, and the bdellovibrio particulate agent is stable in chemical performances, low in cost and long in storage time, and is applicable to multiple industries such as aquatic product culture, livestock and poultry culture and the like.

The invention discloses the application of bdellovibrio in removing the commonly seen pathogen in poultry, eggs and meat; the poultry, eggs and meat with pathogen are treated by bdellovibrio liquid in a spraying, rubbing or soaking way; when the soaking method is adopted, the concentration of the bdellovibrio at least reaches 10<2>puf/ml; when the spraying or the rubbing way is adopted and the use of bdellovibrio liquid with concentration equal to or more than 10<2>pfu/ml, the consumption at least reaches 15ml/kg. The disclosed two units of bdellovibrio can be used independently, and the effect after the two units are mixed is better. The application of the invention is convenient in use, safe, has no side effect, obvious effect in removing commonly seen pathogen in poultry, eggs and meat, germ clearing rate of more than 90 percent, is applicable to the whole process from cultivation to dining including poultry, egg and meat processing, directing improves the product quality, and very significant improving food safety and ensuring the health of human beings.

The invention discloses a microbial preparation for preventing and treating mastitis and applications thereof. Bdellovibrio telotroch and Bdellovibrio plastid are prepared into the microbial preparation for preventing and treating mastitis. By detection, the microbial preparation has favorable functions for preventing and treating mastitis of pigs, sheep and cows. The microbial preparation using the mixture of the Bdellovibrio telotroch and the Bdellovibrio plastid for controlling and eliminating mastitis pathogen has the advantages of quick effect taking and strong activity of the telotroch and the advantages of easy preparation and storage, long sterilization action time and strong environmental resistance. Since Bdellovibrio does not have toxic or side effect on cows, the microbial preparation prepared from the Bdellovibrio telotroch and the Bdellovibrio plastid is suitable for large-size factory culture of pigs, sheep and cows, and can provide a novel method for green processing and production of meat products and milk products and for preventing and treating mastitis pathogen.