29 results about "Pathogenic vibrio" patented technology

The invention provides acinetobacter for culturing prawns in saline-alkali water and application of the acinetobacter. The preservation number of the provided acinetobacter is CGMCC (China General Microbiological Culture Collection Center) No.36066. The acinetobacter soilis strain provided by the invention has remarkable comprehensive application advantages, can still keep higher growth activity and metabolic capability in a low-temperature environment, and continuously plays a water body purification function. Ammonia nitrogen and nitrite nitrogen in a water body can be efficiently degraded, the environment of the aquatic water body is remarkably improved by promoting the nitrogen circulation process, and harm caused by nitrogen pollution is reduced. In addition, the strain can effectively inhibit and prevent reproduction of pathogenic vibrios and reduce the occurrence risk of bacterial diseases of aquatic animals. The strain is high in safety, does not cause poison or stress reaction to bred animals even under the condition of high-concentration use, and has good ecological safety and environmental friendliness.

The application discloses a broad-spectrum short tail phage PhLNJZ2 targeting multi-drug resistant Vibrio and an application thereof, and belongs to the technical field of microorganisms. The phage PhLNJZ2 is preserved in the China Center for Type Culture Collection on October 20, 2025, the preservation address is Wuhan, China, the preservation number is CCTCC NO: M 20252251, and the classification and naming is Vibrio phage PhLNJZ2. The phage is screened from a multi-drug resistant Vibrio alginolyticus clinical isolate which is resistant to more than 7 kinds of antibiotics, exhibits high efficient and broad-spectrum lytic activity on three main waterborne pathogenic Vibrio, i.e., Vibrio alginolyticus, Vibrio parahaemolyticus and Vibrio harveyi, and the total lytic rate reaches 81.25%. The application further provides a composition containing the phage, a feed additive and an application of the phage in preparation of a medicine for preventing and controlling waterborne multi-drug resistant and mixed Vibrio disease or an environmental disinfectant.

The invention discloses an application of nomuraea sp. 18B9 in resisting pathogenic vibrio. The nomuraea sp. 18B9 can be used for resisting pathogenic vibrio. The vibrio is not limited to vibrio candersonii, vibrio mimicus and vibrio parahaemolyticus.

The invention discloses a TaqMan probe qPCR (quantitative polymerase chain reaction) detection primer group for detecting pathogenic vibrio alginolyticus, a kit and application, and belongs to the technical field of molecular biology and pathogenic microorganism detection. The method comprises the following steps: designing a pair of specific primers and probes by taking the vops gene of the vibrio alginolyticus as a new target, connecting the vops gene with plasmids by amplifying the vops gene, constructing a recombinant plasmid standard substance, carrying out qPCR detection on the copy number of the vops gene of the vibrio alginolyticus by utilizing the designed specific primers and probes, and establishing a corresponding relationship between the vibrio alginolyticus and the vops gene. And the vibrio alginolyticus can be quantified through the copy number of the vops gene. On the basis of novel molecular target detection, no amplification signal exists for a detection sample which does not contain vibrio alginolyticus, and the specificity is good; the method is rapid and accurate in detection and high in sensitivity, the lower detection limit is as low as 100-level copies, non-specific amplification can be avoided, and operation is simple and rapid.

The invention relates to the field of microbiology, in particular to lactobacillus plantarum A3 and application thereof. The lactobacillus plantarum is named as lactobacillus plantarum A3, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 2026320, and the preservation unit is China Center for Type Culture Collection. According to the invention, a safe lactobacillus plantarum A3 is successfully separated and obtained, and a novel bacteriocin Plantaricin A3 is identified from the safe lactobacillus plantarum A3; the bacteriocin Plantaricin A3 has excellent thermal stability, pH stability and protease hydrolysis resistance, is wide in antibacterial spectrum, particularly has a remarkable effect on aquatic pathogenic vibrio, and has a wide application prospect in the fields of food, feed and aquaculture.

The invention relates to the technical field of microorganisms, and particularly discloses a streptomyces rust red strain JQ021 and application thereof. The strain is preserved in the China Center for Type Culture Collection on October 9, 2025, and the preservation number of the strain is CCTCC NO: M 20252186. The strain JQ021, a culture of the strain JQ021 and a metabolic extract produced by the strain JQ021 show efficient and broad-spectrum inhibitory activity on various aquatic pathogenic vibrios, especially vibrio parahaemolyticus, vibrio alginolyticus and vibrio Ewana. The invention also relates to a microbial preparation and an antibacterial composition containing the strain or the metabolic extract, and an application of the microbial preparation and the antibacterial composition in preparation of a preparation for preventing and treating aquaculture vibriosis. The strain provides valuable microbial resources for developing safe and efficient antibiotic substitutes.

The invention discloses an application of Schimannella sp. DY612-18-2 for resisting pathogenic vibrio and a preparation method of the Schimannella sp. DY612-18-2. The vibrio is not limited to vibrio harveyi, vibrio mimicus and vibrio parahaemolyticus.

The invention provides a strain of Reichenach sp. H6 and application thereof, the strain has a 16S rRNA gene sequence as shown in SEQ ID NO: 1, is preserved in Guangdong Microbial Culture Collection Center, and has a preservation number of GDMCC No: 66012. The invention also provides a method for preparing the strain of Reichenach sp. H6 and application thereof. The strain is mainly used for enhancing the heat tolerance of the coral. The screened strain is clear in classification status and excellent in hereditary stability, has broad-spectrum complex polysaccharide degradation capacity, and still keeps core biological characteristics after five generations of passage. The strain has a remarkable regulation and control effect on coral co-epiphytic bacterial colony microecology, and the content of metabolites with inhibitory activity on pathogenic vibrios in coral microecology can be remarkably increased; the method is high in environmental adaptability, has a remarkable physiological status relieving effect on corals under heat stress, and can effectively improve core survival indexes of the corals under heat stress.

The invention discloses a primer combination for detecting grouper pathogenic vibrio harveyi and application of the primer combination. The primer combination is a nested PCR primer aiming at the pathogenic vibrio harveyi of groupers, comprises a forward outer primer, a reverse outer primer, a forward inner primer and a reverse inner primer, and can efficiently, sensitively and specifically identify the pathogenic vibrio harveyi in groupers.

The present application relates to the field of microbiological technology, and specifically discloses a strain of Streptomyces rutilocolore JQ021 and its application. The strain has been preserved in the China Center for Type Culture Collection on October 9, 2025, and the preservation number is CCTCC NO: M 20252186. The strain JQ021 and its culture, metabolic extract produced therefrom all exhibit high and broad-spectrum inhibitory activity against various aquatic pathogenic Vibrio, especially Vibrio parahaemolyticus, Vibrio alginolyticus and Vibrio owensii. The present application also relates to a microbial preparation and an antibacterial composition comprising the strain or the metabolic extract, and the application thereof in preparing a preparation for preventing and treating aquatic vibrio disease. The strain provides a valuable microbial resource for developing safe and efficient antibiotic substitutes.

The invention relates to the technical field of disease prevention and control in aquaculture, in particular to a fishery broad-spectrum vibrio oral vaccine based on reverse vaccinology as well as a preparation method and application of the fishery broad-spectrum vibrio oral vaccine. Specifically, on the basis of a reverse vaccinology technology, generic proteome analysis is carried out on common pathogenic vibrios in fish culture, and protein with homology is screened. Through comprehensive analysis of key factors such as subcellular localization, antigenicity, immunogenicity and adhesion, candidate antigens with high potential are further screened out. On the basis, an immunoinformatics method is utilized to carry out epitope prediction analysis on candidate antigens, peptide fragments rich in high-density epitopes are screened out, and the multi-epitope recombinant antigen oral vaccine taking bacillus subtilis as a carrier is constructed according to the peptide fragments. The oral delivery efficiency is enhanced by adopting an alginate microencapsulation process, and feeding of sebastes schlegeli shows that the vaccine has an excellent immune effect, can be used for anti-vibrio drugs, and has a good practical application value.

The invention relates to application of gallic acid in treatment of penaeus vannamei boone glass seedling diseases and an experimental method of gallic acid in treatment of penaeus vannamei boone glass seedling diseases, and belongs to the technical field of experimental animal production. Experiments determine that when the minimum inhibitory concentration of gallic acid to glass seedling disease pathogenic vibrio V.Para.TS-GE is 1000 [mu] g / mL and the minimum bactericidal concentration of gallic acid is 2000 [mu] g / mL, and the concentration of gallic acid is 200 [mu] g / mL, the survival rate of penaeus vannamei seedlings infected with glass seedling diseases can be effectively improved, the death rate can be reduced, and the problem of drug resistance existing in current antibiotic prevention and control can be effectively solved.

The present invention discloses an aquaculture denitrification microcapsule, a preparation method, and an application thereof, and relates to the fields of aquaculture and biotechnology. The Lactobacillus casei provided by the present invention can secrete acidic metabolites, which can inhibit pathogenic Vibrio, helping to reduce the occurrence of diseases in aquaculture. Lactobacillus casei has the ability to efficiently degrade ammonia nitrogen and nitrite nitrogen in the aquaculture environment, and can sustainably achieve efficient cleaning of the aquaculture environment. In addition, a denitrification microcapsule is provided, which creates a more favorable microenvironment for the efficient denitrification strain, thereby more stably exerting repeated denitrification performance, and the denitrification microcapsules can be reused.

The present application belongs to the technical field of microbial prevention and treatment, and particularly relates to a bacteriophage for degrading Vibrio biofilm across hosts and application thereof. The bacteriophage is VpaR-108, which has been sent to China Center for Type Culture Collection (address: Wuhan University, LuoJia Mountain, Wuchang, Wuhan, Hubei Province), and the preservation date is October 24, 2025, and the preservation number is CCTCC NO: M 20252328. A novel virulent bacteriophage VpaR-108 is screened in the present application, the bacteriophage has the ability to degrade biofilms of multiple Vibrio strains and mixed biofilms across hosts, and can significantly reduce the stability of the biofilm. Therefore, the bacteriophage has a broad application prospect in the aquaculture industry, and has important practical application value for the prevention and control of pathogenic Vibrio in marine aquaculture animals.

This invention discloses a method for predicting the dosage of potassium persulfate compound salt in the factory farming of Litopenaeus vannamei, belonging to the field of intelligent disinfection control in aquaculture. The method includes: acquiring the biomechanical parameters of potassium persulfate compound salt's inactivation of pathogenic Vibrio, reactive oxygen species attenuation parameters, Vibrio growth kinetic parameters, and shrimp safety threshold; constructing a microbial growth sub-model, a disinfectant inactivation sub-model, and a disinfectant concentration attenuation sub-model, respectively; and dynamically coupling these three sub-models into a dosage prediction model in system dynamics software. Real-time physicochemical parameters of the aquaculture water are input into the model, and the initial dosage concentration of potassium persulfate compound salt, effective sterilization duration, pathogen regrowth warning, and dosing interval are dynamically output. This invention can dynamically and accurately predict the disinfectant dosage based on the water quality parameters at the aquaculture site, achieving efficient Vibrio control while ensuring shrimp safety, and is suitable for intelligent disinfection management in factory shrimp farming.

The application belongs to the technical field of genetic engineering, and particularly relates to a single-domain antibody targeting Vibrio pathogenic PirA toxin protein and application thereof in immunodetection. The single-domain antibody targeting Vibrio pathogenic PirA toxin protein screened in the application is A02 or A15, the amino acid sequence of the single-domain antibody A02 is shown as SEQ ID NO. 1, and the amino acid sequence of the single-domain antibody A15 is shown as SEQ ID NO. 2. The single-domain antibody provided in the application is high in stability, strong in stress resistance, and good in specificity, and has good application value. The single-domain antibody provided in the application can be well applied to preparation of a detection product for detection of a shrimp AHPND pathogen.

The application discloses application of chebulic acid or chebulinic acid in preparation of a medicine against aquatic pathogenic bacteria. The active tracking method is used to separate, purify and identify the active components in the extract of Terminalia chebula against Vibrio pathogenic bacteria, and the active components are identified as chebulic acid and chebulinic acid. The chebulic acid and the chebulinic acid are simple to prepare, have small toxic and side effects, and have strong bacteriostatic effects, especially on Vibrio parahaemolyticus. The experimental results show that the 96h half lethal concentrations of the chebulic acid and the chebulinic acid on fish are 561.653mg / L and 525.011mg / L respectively, which are 22.47 and 21.00 times of the minimum bacteriostatic concentration 25mg / L of the chebulic acid and the chebulinic acid on Vibrio parahaemolyticus, and the chebulic acid and the chebulinic acid are safe to fish and have broad-spectrum bacteriostatic effects on Pseudomonas aeruginosa, Aeromonas veronii, Aeromonas hydrophila, Vibrio alginolyticus, Vibrio vulnificus and Vibrio parahaemolyticus, and have good application prospects in the field of new environment-friendly fish medicines.

The present invention provides a method for preparing a medicament for improving the survival rate of shrimp infected with acute hepatopancreatic necrosis, belonging to the technical field of shrimp aquaculture disease prevention and control. The present invention utilizes ursodeoxycholic acid (UDCA), a lipid metabolism regulator, to regulate the metabolic pathways of infected shrimp, significantly reducing the damage caused by pathogenic Vibrio infection to shrimp. Experiments have shown that UDCA, whether injected or added to feed, significantly reduces symptoms and mortality in shrimp infected with acute hepatopancreatic necrosis, demonstrating its potential for preventing and treating acute hepatopancreatic necrosis in shrimp.

The application provides application of obeticholic acid in prevention and treatment of acute hepatopancreas necrosis disease of shrimp, and belongs to the technical field of shrimp breeding disease prevention and control. The application adopts a kind of bile acid metabolism regulator, obeticholic acid (OCA), controls the metabolism of bile acid of diseased shrimp, reduces the accumulation of bile acid in the hepatopancreas of shrimp, and reduces the hepatopancreas damage caused by pathogenic vibrio infection. No matter injection or adding to feed, obeticholic acid significantly reduces the symptoms and mortality of shrimp after infection of acute hepatopancreas necrosis disease, which shows that obeticholic acid has certain application value in prevention and treatment of acute hepatopancreas necrosis disease of shrimp.

The application discloses a pathogenic Vibrio Pir B virulence protein binding peptide P2 and application thereof. The small-molecule binding peptide P2 has high affinity with the Pir B virulence protein and has an amino acid sequence of LGSPLLTYGRPQ. The application screens the binding peptide with high affinity with the pathogenic Vibrio Pir B virulence protein from a random peptide library through phage display technology, and the binding peptide is chemically synthesized. After injection of the binding peptide, the survival rate of shrimps after infection with Vibrio can be effectively improved, and the shrimps have a remarkable protection effect in shrimp culture. The binding peptide can efficiently bind to the pathogenic Vibrio Pir B virulence protein, and effectively reduce the virulence of the Pir B protein. In addition, the method can effectively reduce the use of antibiotics in healthy shrimp culture, reduce the generation of drug-resistant strains, and has a good application prospect in the prevention and treatment of shrimp AHPND.

The invention provides a bacteriophage vBVneMXG7 and application thereof, and belongs to the technical field of microorganisms. The phage vBVneMXG7 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the phage vBVneMXG7 is CGMCC No.46367. The bacteriophage vBVneMXG7 provided by the invention has relatively strong tolerance to temperature and pH and can adapt to various environments, a genome of the bacteriophage vBVneMXG7 does not contain virulence genes and antibiotic resistance genes, so that the bacteriophage vBVneMXG7 has good biological safety, also has the capability of efficiently splitting pathogenic vibrios, can effectively pertinently solve the disease problem caused by the pathogenic vibrios, and has a good application prospect. The traditional Chinese medicine composition has great advantages in the aspect of treating aquaculture bacterial diseases, does not cause bacterial drug resistance and resistance, does not cause secondary pollution to the culture environment, and is green and environment-friendly.

This invention discloses a broad-spectrum short-tailed bacteriophage, PhLNJZ2, targeting multidrug-resistant Vibrio and its applications, belonging to the field of microbial technology. The bacteriophage PhLNJZ2 was deposited on October 20, 2025, at the China Center for Type Culture Collection (CCTCC), Wuhan, China, with accession number CCTCC NO:M 20252251, and classified as follows: Vibrio Phage PhLNJZ2. This bacteriophage was screened from a clinical isolate of a multidrug-resistant Vibrio alginolyticus resistant to more than seven antibiotics. It exhibits highly efficient and broad-spectrum lytic activity against three major aquatic pathogenic Vibrio species: Vibrio alginolyticus, Vibrio parahaemolyticus, and Vibrio harveyi, with a total lysis rate of 81.25%. This invention also provides compositions containing this bacteriophage, feed additives, and their application in the preparation of drugs or environmental disinfectants for the prevention and control of multidrug-resistant and mixed vibrio infections in aquatic organisms.

The invention relates to the technical field of biology, in particular to bacillus for inhibiting aquatic pathogenic vibrio and a composition and application thereof. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 25, 2025, and the preservation number is CGMCC No.35730. The bacterial strain can secrete a novel antibacterial peptide BS-25001PRO, and has a strong inhibition effect on various aquatic pathogenic bacteria such as vibrio parahaemolyticus, vibrio alginolyticus and vibrio harveyi, and the MIC of the bacterial strain is as low as 0.25 mu g / mL. The invention also discloses that the bacillus and the vibrio parahaemolyticus bacteriophage RDP-VP21516 are combined for use, so that a remarkable synergistic antibacterial effect is achieved. In addition, the invention provides a bacillus sustained-release preparation taking chitosan-sodium alginate as a carrier, and the bacillus sustained-release preparation can maintain the activity for 28 days in a water body for a long time. Safety tests prove that the compound is safe and non-toxic to zebrafish and plankton. Aquaculture tests show that the method can effectively prevent and control the vibriosis of prawns, improve the survival rate by more than 18%, reduce the feed coefficient by 0.3, and significantly degrade ammonia nitrogen in water.

The invention belongs to the technical field of microbial control, and particularly relates to a cross-host bacteriophage for degrading a vibrio biomembrane and application of the cross-host bacteriophage. The phage is VpaR-108 and is sent to the China Center for Type Culture Collection (the address is Wuhan University, Luojia Hill, Wuchang, Wuhan City, Hubei Province), the collection date is October 24, 2025, and the collection number is CCTCC NO: M 20252328. A novel virulent bacteriophage VpaR-108 is obtained through screening, the bacteriophage has the capacity of degrading multiple vibrio biological membranes and mixed biological membranes of the vibrio biological membranes in a cross-host mode, and the stability of the biological membranes can be remarkably reduced. Therefore, the bacteriophage has a wide application prospect in the aquaculture industry, and has an important practical application value in prevention and control of pathogenic vibrio of mariculture animals.

The invention provides a method for detecting typical pathogenic vibrios of laver. Two crRNAs, two pairs of RPA primers and a probe are provided for two vibrios respectively. After RPA primer screening and crRNA screening are carried out, RPA amplification is firstly carried out according to an RPA reaction system, then CRISPR reaction and LFD detection are carried out, and specificity and sensitivity experiments are carried out. According to the RPA-CRISPR-LbaCas12a-LFD detection method provided by the invention, the reaction can be completed in 40 minutes under the condition of being close to the body temperature. The method has the advantages of short detection time, low requirements on instruments, equipment and users, extremely high specificity and sensitivity, extremely high application value in field detection of vibrio in a water environment, incomparable advantages in early prevention and control of pathogens of laver and even an aquaculture system, and wide application prospect. The positive significance is realized in the aspect of protecting the aquaculture industry in China.

The invention relates to the technical field of microorganisms, and provides a vibrio alginolyticus phage and application thereof in order to screen and accumulate a phage with a good lysis effect, the latin classification name of the vibrio alginolyticus phage is Vibrio alginolyticus phage, the vibrio alginolyticus phage is named as GYN01, the vibrio alginolyticus phage is preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC No.46683, and the vibrio alginolyticus phage is named as GYN01. The invention relates to an icosahedron-shaped tail sheath, which has a typical tadpole-shaped composite symmetrical structure, comprises an icosahedron head part and a retractable tail sheath, a tail plate visible at the tail end of the tail part, a non-retractable tail part and a hollow tail tube visible in the center of the tail sheath, has good temperature stability and a wider acid-base tolerance range, and is sensitive to ultraviolet rays; the compound has good cracking capacity and killing effect on pathogenic vibrio alginolyticus, is good in safety and environment-friendly, and can be used for preventing and treating vibrio alginolyticus diseases in the aquaculture process.

The invention relates to the technical field of biology, and particularly discloses fungus and diatom symbiont microspheres for improving the immune function of cage culture stichopus japonicus and improving water quality. Core functional components of the fungus and diatom symbiont microspheres are cordyceps militaris fungi and Ampelopsis coffee, and in addition, in order to improve the biological activity of the symbiont microspheres and the adhesion of the symbiont microspheres to a netting, pullulan, recombinant mussel mucin, a folium cortex eucommiae extract and a curcuma zedoary extract are also added in the preparation process of the microspheres. The functional components of the symbiont microsphere disclosed by the invention are mutually beneficial, common and synergistic, the non-specific immune function of the stichopus japonicus can be comprehensively improved by enhancing the activity of immune enzyme and digestive enzyme, and meanwhile, the contents of nitrogen phosphorus, nitrite, organic pollutants and pathogenic vibrio in an aquaculture water body can also be reduced.