45 results about "Pathogenic vibrio" patented technology

The invention belongs to the technical field of molecular biology, and particularly relates to a nucleic acid sequence combination for simultaneously detecting various pathogenic vibrios, an RT-PCR (reverse transcription-polymerase chain reaction) kit and a use method of the RT-PCR kit, and the nucleic acid sequence combination is shown as SEQ ID No.1-SEQ ID No.19. The RT-PCR kit comprises RT-PCR primer probes, the primer probes comprise forward and reverse amplification primers and detection probes respectively aiming at vibrio cholerae, vibrio parahaemolyticus, vibrio fluvialis, vibrio mimicus, vibrio vulnificus and vibrio alginolyticus, and corresponding sequences in SEQ ID No.1-SEQ ID No.19 are adopted. The kit disclosed by the invention can be used for rapidly detecting and identifying the six vibrios at the same time; the kit has high accuracy, specificity and sensitivity, and is suitable for clinical detection of infected samples.

The invention discloses an aquaculture denitrification microcapsule as well as a preparation method and application thereof, and relates to the technical field of aquaculture and biology. The lactobacillus casei provided by the invention can secrete acid metabolites, and the acid metabolites can inhibit pathogenic vibrio, so that the occurrence of diseases in aquaculture can be reduced. Lactobacillus casei has the efficacy of efficiently degrading ammonia nitrogen and nitrite nitrogen in the aquaculture environment, and can continuously realize efficient cleaning of the aquaculture environment. In addition, the invention also provides a denitrification microcapsule which creates a more favorable microenvironment for the efficient denitrification strain, so that the repeated denitrification performance is more stably exerted, and the denitrification microcapsule can be repeatedly utilized.

The invention belongs to the field of gene engineering, and particularly relates to an ecdysone receptor gene LvECR and application thereof. The base sequence of the ecdysone receptor gene LvECR is as shown in SEQ ID NO: 1. The amino acid sequence of the protein encoded by the LvECR gene is as shown in SEQ ID NO: 2. The invention also discloses an application of the ecdysone receptor gene LvECR in inhibition of pathogenic vibrio infection. It is verified for the first time that the prawn ecdysone receptor gene LvECR plays an important role in prawn acute hepatopancreatic necrosis, disease occurrence can be aggravated by inhibiting expression of the coding gene of the prawn ecdysone receptor gene LvECR, disease symptoms can be reduced by injecting in-vitro recombinant protein of the factor, and the huge application potential of the prawn ecdysone receptor gene LvECR in disease prevention and control is shown.

The invention provides acinetobacter for culturing prawns in saline-alkali water and application of the acinetobacter. The preservation number of the provided acinetobacter is CGMCC (China General Microbiological Culture Collection Center) No.36066. The acinetobacter soilis strain provided by the invention has remarkable comprehensive application advantages, can still keep higher growth activity and metabolic capability in a low-temperature environment, and continuously plays a water body purification function. Ammonia nitrogen and nitrite nitrogen in a water body can be efficiently degraded, the environment of the aquatic water body is remarkably improved by promoting the nitrogen circulation process, and harm caused by nitrogen pollution is reduced. In addition, the strain can effectively inhibit and prevent reproduction of pathogenic vibrios and reduce the occurrence risk of bacterial diseases of aquatic animals. The strain is high in safety, does not cause poison or stress reaction to bred animals even under the condition of high-concentration use, and has good ecological safety and environmental friendliness.

The invention provides a method for detecting three pathogenic vibrios of penaeus vannamei. According to the method, specific primers are designed by using toxR genes of vibrio fluvialis, flaA genes of vibrio anguillarum and pyrH genes of vibrio alginolyticus, and sensitivity test is carried out. The lowest detectable concentrations of the three vibrios are 5.21 * 10 < 2 > cfu / mL, 2.70 * 10 < 4 > cfu / mL and 2.48 * 10 < 2 > cfu / mL, and the sensitivity is high. The three vibrios are accurately and simultaneously identified through a multiple PCR method, and the identification efficiency is improved. Meanwhile, the lowest detection concentrations of the DNA of the three vibrios identified and extracted by using a real-time fluorescent quantitative PCR (qRT-PCR) technology are 1.0 * 10 <-6 > nmol / L, 1.0 * 10 <-7 > nmol / L and 1.0 * 10 <-8 > nmol / L respectively. Compared with the two methods, the common PCR technology has lower requirements on instruments and operation, so that the common PCR technology is more suitable for the diagnosis of the vibrio infection of the penaeus vannamei. The qRT-PCR technology is more suitable for detection cases with higher sensitivity requirements. By using the method, an efficient and reliable means is provided for diagnosing the bacterial diseases of the penaeus vannamei boone.

The application discloses a broad-spectrum short tail phage PhLNJZ2 targeting multi-drug resistant Vibrio and an application thereof, and belongs to the technical field of microorganisms. The phage PhLNJZ2 is preserved in the China Center for Type Culture Collection on October 20, 2025, the preservation address is Wuhan, China, the preservation number is CCTCC NO: M 20252251, and the classification and naming is Vibrio phage PhLNJZ2. The phage is screened from a multi-drug resistant Vibrio alginolyticus clinical isolate which is resistant to more than 7 kinds of antibiotics, exhibits high efficient and broad-spectrum lytic activity on three main waterborne pathogenic Vibrio, i.e., Vibrio alginolyticus, Vibrio parahaemolyticus and Vibrio harveyi, and the total lytic rate reaches 81.25%. The application further provides a composition containing the phage, a feed additive and an application of the phage in preparation of a medicine for preventing and controlling waterborne multi-drug resistant and mixed Vibrio disease or an environmental disinfectant.

The invention discloses an application of nomuraea sp. 18B9 in resisting pathogenic vibrio. The nomuraea sp. 18B9 can be used for resisting pathogenic vibrio. The vibrio is not limited to vibrio candersonii, vibrio mimicus and vibrio parahaemolyticus.

The invention discloses a TaqMan probe qPCR (quantitative polymerase chain reaction) detection primer group for detecting pathogenic vibrio alginolyticus, a kit and application, and belongs to the technical field of molecular biology and pathogenic microorganism detection. The method comprises the following steps: designing a pair of specific primers and probes by taking the vops gene of the vibrio alginolyticus as a new target, connecting the vops gene with plasmids by amplifying the vops gene, constructing a recombinant plasmid standard substance, carrying out qPCR detection on the copy number of the vops gene of the vibrio alginolyticus by utilizing the designed specific primers and probes, and establishing a corresponding relationship between the vibrio alginolyticus and the vops gene. And the vibrio alginolyticus can be quantified through the copy number of the vops gene. On the basis of novel molecular target detection, no amplification signal exists for a detection sample which does not contain vibrio alginolyticus, and the specificity is good; the method is rapid and accurate in detection and high in sensitivity, the lower detection limit is as low as 100-level copies, non-specific amplification can be avoided, and operation is simple and rapid.

The invention relates to the field of microbiology, in particular to lactobacillus plantarum A3 and application thereof. The lactobacillus plantarum is named as lactobacillus plantarum A3, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 2026320, and the preservation unit is China Center for Type Culture Collection. According to the invention, a safe lactobacillus plantarum A3 is successfully separated and obtained, and a novel bacteriocin Plantaricin A3 is identified from the safe lactobacillus plantarum A3; the bacteriocin Plantaricin A3 has excellent thermal stability, pH stability and protease hydrolysis resistance, is wide in antibacterial spectrum, particularly has a remarkable effect on aquatic pathogenic vibrio, and has a wide application prospect in the fields of food, feed and aquaculture.

The invention provides application of ursodesoxycholic acid in preparation of a medicament for improving the survival rate of acute hepatopancreatic necrosis of prawns, and belongs to the technical field of disease prevention and control in prawn culture. According to the invention, a lipid metabolism regulator ursodesoxycholic acid (UDCA) is adopted, and the damage of pathogenic vibrio infection to prawns is obviously reduced by regulating and controlling the metabolic pathway of infected prawn bodies. Tests prove that no matter the ursodesoxycholic acid is injected or added into the feed for feeding, the symptoms and the death rate of the prawns infected with the acute hepatopancreatic necrosis disease are remarkably reduced, and the ursodesoxycholic acid has a certain prevention and treatment effect in the prevention and treatment of the acute hepatopancreatic necrosis disease of the prawns.

The invention relates to the technical field of microorganisms, and particularly discloses a streptomyces rust red strain JQ021 and application thereof. The strain is preserved in the China Center for Type Culture Collection on October 9, 2025, and the preservation number of the strain is CCTCC NO: M 20252186. The strain JQ021, a culture of the strain JQ021 and a metabolic extract produced by the strain JQ021 show efficient and broad-spectrum inhibitory activity on various aquatic pathogenic vibrios, especially vibrio parahaemolyticus, vibrio alginolyticus and vibrio Ewana. The invention also relates to a microbial preparation and an antibacterial composition containing the strain or the metabolic extract, and an application of the microbial preparation and the antibacterial composition in preparation of a preparation for preventing and treating aquaculture vibriosis. The strain provides valuable microbial resources for developing safe and efficient antibiotic substitutes.

The invention discloses an application of Schimannella sp. DY612-18-2 for resisting pathogenic vibrio and a preparation method of the Schimannella sp. DY612-18-2. The vibrio is not limited to vibrio harveyi, vibrio mimicus and vibrio parahaemolyticus.

The invention provides a strain of Reichenach sp. H6 and application thereof, the strain has a 16S rRNA gene sequence as shown in SEQ ID NO: 1, is preserved in Guangdong Microbial Culture Collection Center, and has a preservation number of GDMCC No: 66012. The invention also provides a method for preparing the strain of Reichenach sp. H6 and application thereof. The strain is mainly used for enhancing the heat tolerance of the coral. The screened strain is clear in classification status and excellent in hereditary stability, has broad-spectrum complex polysaccharide degradation capacity, and still keeps core biological characteristics after five generations of passage. The strain has a remarkable regulation and control effect on coral co-epiphytic bacterial colony microecology, and the content of metabolites with inhibitory activity on pathogenic vibrios in coral microecology can be remarkably increased; the method is high in environmental adaptability, has a remarkable physiological status relieving effect on corals under heat stress, and can effectively improve core survival indexes of the corals under heat stress.

The invention discloses a bacillus subtilis and application thereof, and particularly provides the bacillus subtilis and a bacillus subtilis culture supernatant obtained through the bacillus subtilis, the bacillus subtilis is classified and named as Bacillus subtilis SD24, the preservation unit is China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO.M20242253, and the preservation time is October 21, 2024. Experiments prove that when the bacillus subtilis and the culture supernatant thereof are used in aquaculture, reproduction of harmful bacteria (aquatic pathogenic vibrio) can be well inhibited, so that the water quality is improved. Experimental results also prove that the bacillus subtilis culture supernatant still has good antibacterial stability after being placed for 30 days, and is convenient to store.

The invention relates to the technical field of antibacterial peptides, in particular to an antibacterial peptide mb217 as well as a modified peptide and application thereof, and researches find that the antibacterial peptide mb217 and the modified peptide thereof have a remarkable inhibition effect on various marine pathogenic vibrios, the infection rate of the vibrios is effectively reduced, and diseases in aquaculture are reduced; according to the two modified peptides of the antibacterial peptide mb217 provided by the invention, the molecular structure of the antibacterial peptide is optimized, so that the stability of the antibacterial peptide in a high-salt environment is improved, the antibacterial peptide can effectively play an antibacterial role in seawater or a high-salt water body in the future, the common high-salt environment in aquaculture is effectively dealt with, and a more stable biological prevention and control means is provided for the breeding industry.

The invention discloses a primer combination for detecting grouper pathogenic vibrio harveyi and application of the primer combination. The primer combination is a nested PCR primer aiming at the pathogenic vibrio harveyi of groupers, comprises a forward outer primer, a reverse outer primer, a forward inner primer and a reverse inner primer, and can efficiently, sensitively and specifically identify the pathogenic vibrio harveyi in groupers.

The present application relates to the field of microbiological technology, and specifically discloses a strain of Streptomyces rutilocolore JQ021 and its application. The strain has been preserved in the China Center for Type Culture Collection on October 9, 2025, and the preservation number is CCTCC NO: M 20252186. The strain JQ021 and its culture, metabolic extract produced therefrom all exhibit high and broad-spectrum inhibitory activity against various aquatic pathogenic Vibrio, especially Vibrio parahaemolyticus, Vibrio alginolyticus and Vibrio owensii. The present application also relates to a microbial preparation and an antibacterial composition comprising the strain or the metabolic extract, and the application thereof in preparing a preparation for preventing and treating aquatic vibrio disease. The strain provides a valuable microbial resource for developing safe and efficient antibiotic substitutes.

The invention relates to the technical field of disease prevention and control in aquaculture, in particular to a fishery broad-spectrum vibrio oral vaccine based on reverse vaccinology as well as a preparation method and application of the fishery broad-spectrum vibrio oral vaccine. Specifically, on the basis of a reverse vaccinology technology, generic proteome analysis is carried out on common pathogenic vibrios in fish culture, and protein with homology is screened. Through comprehensive analysis of key factors such as subcellular localization, antigenicity, immunogenicity and adhesion, candidate antigens with high potential are further screened out. On the basis, an immunoinformatics method is utilized to carry out epitope prediction analysis on candidate antigens, peptide fragments rich in high-density epitopes are screened out, and the multi-epitope recombinant antigen oral vaccine taking bacillus subtilis as a carrier is constructed according to the peptide fragments. The oral delivery efficiency is enhanced by adopting an alginate microencapsulation process, and feeding of sebastes schlegeli shows that the vaccine has an excellent immune effect, can be used for anti-vibrio drugs, and has a good practical application value.

The invention discloses a marker and a method for detecting five pathogenic vibrios of aquatic animals and application, and belongs to the technical field of pathogenic vibrio detection. The coding gene of the difunctional diguanylate cyclase / phosphodiesterase is found for the first time and has specificity in five aquatic animal pathogenic vibrios, and rapid detection of the five aquatic animal pathogenic vibrios can be achieved through specific primers designed for the gene; except vibrio harveyi, the other four pathogenic vibrios all have two pairs of specific primers, so that the purpose of dual verification can be achieved during detection, and false negative caused by gene deletion can be effectively avoided; the provided method is high in specificity; the detection time is short; the operation is simple and easy; the detection cost is low; the lowest detection limit is low, and the lowest detection limit range of the nine pairs of specific primers is 0.0157 ng / [mu] L-6. 1813 ng / [mu] L; the method is suitable for large-scale daily detection of basic farms.

The invention relates to application of gallic acid in treatment of penaeus vannamei boone glass seedling diseases and an experimental method of gallic acid in treatment of penaeus vannamei boone glass seedling diseases, and belongs to the technical field of experimental animal production. Experiments determine that when the minimum inhibitory concentration of gallic acid to glass seedling disease pathogenic vibrio V.Para.TS-GE is 1000 [mu] g / mL and the minimum bactericidal concentration of gallic acid is 2000 [mu] g / mL, and the concentration of gallic acid is 200 [mu] g / mL, the survival rate of penaeus vannamei seedlings infected with glass seedling diseases can be effectively improved, the death rate can be reduced, and the problem of drug resistance existing in current antibiotic prevention and control can be effectively solved.

The present invention relates to the technical field of antimicrobial peptides, and specifically to an antimicrobial peptide mb217, a modified peptide thereof, and applications thereof. Studies have found that the antimicrobial peptide mb217 and its modified peptides have significant inhibitory effects on a variety of marine pathogenic Vibrio, effectively reducing the infection rate of Vibrio and the occurrence of diseases in aquaculture. The two modified peptides of the provided antimicrobial peptide mb217 improve its stability in high-salt environments by optimizing its molecular structure, enabling it to effectively exert its antimicrobial effect in seawater or high-salt water bodies in the future, effectively coping with the high-salt environment commonly found in aquaculture, and providing a more stable biological control method for the aquaculture industry.

The present invention discloses an aquaculture denitrification microcapsule, a preparation method, and an application thereof, and relates to the fields of aquaculture and biotechnology. The Lactobacillus casei provided by the present invention can secrete acidic metabolites, which can inhibit pathogenic Vibrio, helping to reduce the occurrence of diseases in aquaculture. Lactobacillus casei has the ability to efficiently degrade ammonia nitrogen and nitrite nitrogen in the aquaculture environment, and can sustainably achieve efficient cleaning of the aquaculture environment. In addition, a denitrification microcapsule is provided, which creates a more favorable microenvironment for the efficient denitrification strain, thereby more stably exerting repeated denitrification performance, and the denitrification microcapsules can be reused.

The invention discloses application of chebulinic acid or chebulinic acid in preparation of drugs for resisting aquatic pathogenic bacteria. According to the invention, active components for resisting pathogenic vibrio in the fructus chebulae extract are separated, purified and identified by adopting an activity tracking mode, and the active components are identified as chebulinic acid and chebulagic acid. The chebulinic acid and the chebulinic acid are simple to prepare and small in toxic and side effects, and have a strong bacteriostatic effect, especially for inhibiting vibrio parahaemolyticus. Experimental results show that the 96-hour median lethal concentrations of chebulagic acid and chebulagic acid to fish are respectively 561.653 mg / L and 525.011 mg / L, which are 22.47 times and 21.00 times of the minimum inhibitory concentration 25mg / L for inhibiting vibrio parahaemolyticus, and the chebulagic acid and the chebulagic acid are safe to the fish, have broad-spectrum antibacterial effect, have inhibition effect on pseudomonas aeruginosa, aeromonas veronii, aeromonas hydrophila, vibrio alginolyticus, vibrio vulnificus, vibrio parahaemolyticus and the like, and can be used for preparing a compound preparation for treating the vibrio parahaemolyticus. Good application prospects are realized in the field of novel environment-friendly fishery medicines.

The present application belongs to the technical field of microbial prevention and treatment, and particularly relates to a bacteriophage for degrading Vibrio biofilm across hosts and application thereof. The bacteriophage is VpaR-108, which has been sent to China Center for Type Culture Collection (address: Wuhan University, LuoJia Mountain, Wuchang, Wuhan, Hubei Province), and the preservation date is October 24, 2025, and the preservation number is CCTCC NO: M 20252328. A novel virulent bacteriophage VpaR-108 is screened in the present application, the bacteriophage has the ability to degrade biofilms of multiple Vibrio strains and mixed biofilms across hosts, and can significantly reduce the stability of the biofilm. Therefore, the bacteriophage has a broad application prospect in the aquaculture industry, and has important practical application value for the prevention and control of pathogenic Vibrio in marine aquaculture animals.

This invention discloses a method for predicting the dosage of potassium persulfate compound salt in the factory farming of Litopenaeus vannamei, belonging to the field of intelligent disinfection control in aquaculture. The method includes: acquiring the biomechanical parameters of potassium persulfate compound salt's inactivation of pathogenic Vibrio, reactive oxygen species attenuation parameters, Vibrio growth kinetic parameters, and shrimp safety threshold; constructing a microbial growth sub-model, a disinfectant inactivation sub-model, and a disinfectant concentration attenuation sub-model, respectively; and dynamically coupling these three sub-models into a dosage prediction model in system dynamics software. Real-time physicochemical parameters of the aquaculture water are input into the model, and the initial dosage concentration of potassium persulfate compound salt, effective sterilization duration, pathogen regrowth warning, and dosing interval are dynamically output. This invention can dynamically and accurately predict the disinfectant dosage based on the water quality parameters at the aquaculture site, achieving efficient Vibrio control while ensuring shrimp safety, and is suitable for intelligent disinfection management in factory shrimp farming.

The application belongs to the technical field of genetic engineering, and particularly relates to a single-domain antibody targeting Vibrio pathogenic PirA toxin protein and application thereof in immunodetection. The single-domain antibody targeting Vibrio pathogenic PirA toxin protein screened in the application is A02 or A15, the amino acid sequence of the single-domain antibody A02 is shown as SEQ ID NO. 1, and the amino acid sequence of the single-domain antibody A15 is shown as SEQ ID NO. 2. The single-domain antibody provided in the application is high in stability, strong in stress resistance, and good in specificity, and has good application value. The single-domain antibody provided in the application can be well applied to preparation of a detection product for detection of a shrimp AHPND pathogen.

The application discloses application of chebulic acid or chebulinic acid in preparation of a medicine against aquatic pathogenic bacteria. The active tracking method is used to separate, purify and identify the active components in the extract of Terminalia chebula against Vibrio pathogenic bacteria, and the active components are identified as chebulic acid and chebulinic acid. The chebulic acid and the chebulinic acid are simple to prepare, have small toxic and side effects, and have strong bacteriostatic effects, especially on Vibrio parahaemolyticus. The experimental results show that the 96h half lethal concentrations of the chebulic acid and the chebulinic acid on fish are 561.653mg / L and 525.011mg / L respectively, which are 22.47 and 21.00 times of the minimum bacteriostatic concentration 25mg / L of the chebulic acid and the chebulinic acid on Vibrio parahaemolyticus, and the chebulic acid and the chebulinic acid are safe to fish and have broad-spectrum bacteriostatic effects on Pseudomonas aeruginosa, Aeromonas veronii, Aeromonas hydrophila, Vibrio alginolyticus, Vibrio vulnificus and Vibrio parahaemolyticus, and have good application prospects in the field of new environment-friendly fish medicines.

The present invention provides a method for preparing a medicament for improving the survival rate of shrimp infected with acute hepatopancreatic necrosis, belonging to the technical field of shrimp aquaculture disease prevention and control. The present invention utilizes ursodeoxycholic acid (UDCA), a lipid metabolism regulator, to regulate the metabolic pathways of infected shrimp, significantly reducing the damage caused by pathogenic Vibrio infection to shrimp. Experiments have shown that UDCA, whether injected or added to feed, significantly reduces symptoms and mortality in shrimp infected with acute hepatopancreatic necrosis, demonstrating its potential for preventing and treating acute hepatopancreatic necrosis in shrimp.

The present invention discloses a Vibrio parahaemolyticus phage with a multivalent lysis spectrum and its application in sea cucumber farming, belonging to the field of biotechnology. The phage has been sent to the China Center for Type Culture Collection for preservation on January 18, 2024. The preservation address is: Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2024134. The phage VPP19 has a good lysis effect on common pathogenic Vibrio in sea cucumber farming, such as Vibrio parahaemolyticus, Vibrio splendidus, Vibrio alginolyticus, Vibrio harveyi and Vibrio robustus. In addition, the Vibrio parahaemolyticus phage VPP19 has high value-added titer, wide lysis spectrum, safety and high efficiency, and good tolerance to temperature and pH. The present invention can effectively prevent diseases caused by common pathogenic Vibrio in sea cucumber farming, and provide excellent strain resources and biological prevention measures for large-scale fermentation production for phage therapy of Vibrio problems in sea cucumbers.