6 results about "Vibrio vulnificus" patented technology

This invention relates to the field of food testing technology, and more particularly to a colorimetric LAMP method for the simultaneous visual detection of Vibrio vulnificus and Vibrio alginolyticus. The method employs a LAMP detection system composed of DNA-functionalized gold nanospheres and gold nanorods for detecting Vibrio vulnificus and Vibrio alginolyticus, respectively, and LAMP primer pairs. This allows for the detection and determination of the following states of Vibrio vulnificus and Vibrio alginolyticus: when neither pathogen is present, the solution is transparent; when both pathogens are present, the solution is purplish-red; when only Vibrio alginolyticus is present, the solution is gray; and when only Vibrio vulnificus is present, the solution is purple. This method can accurately determine the presence or absence of Vibrio vulnificus and Vibrio alginolyticus, and the specific type of bacteria, avoiding misjudgments, missed detections, and inaccurate traceability of these two types of pathogen contamination in food safety monitoring, clinical practice, and aquaculture protection.

The application provides a new bacteriophage for preventing and treating vibrio diseases of aquatic animals and application thereof, the bacteriophage is preserved in China Center for Type Culture Collection on September 4, 2020, and the preservation number is CCTCC M 2020465. The bacteriophage is a new species of bacteriophage of the Caudovirales class and Queuovirinae subfamily, the whole genome of the bacteriophage is OP331229.1 in the NCBI official website GeneBank. The bacteriophage has a wide phage spectrum, can crack 6 different species and genera of vibrio at the same time, can crack 45 strains of Vibrio parahaemolyticus, 22 strains of Vibrio harveyi, 17 strains of Vibrio alginolyticus, 13 strains of Vibrio vulnificus, 11 strains of Vibrio campbellii and 9 strains of Vibrio cholerae at the same time, can more effectively solve the problem of multiple bacterial disease infections in aquaculture, and can be used as a microecological preparation for preventing and treating vibrio diseases such as Vibrio parahaemolyticus, Vibrio harveyi and Vibrio alginolyticus of aquatic animals.

This invention discloses a quadruple Taqman qPCR reagent for detecting foodborne pathogens in aquatic products. The foodborne pathogens are Vibrio parahaemolyticus, Vibrio vulnificus, Shigella, and Staphylococcus aureus. The reagent includes specific primers and Taqman probes corresponding to the four foodborne pathogens. This invention provides a quadruple Taqman qPCR reagent with high efficiency, accuracy, sensitivity, and speed, offering an accurate, rapid, efficient, and economical method for detecting the above four foodborne pathogens in the quality and safety testing of aquatic products and their processed products. This is of great significance for ensuring the safety of aquatic products for consumption in my country.

The application discloses a Pseudomonas australis 94-6Y strain, a bacterial agent and application thereof, and belongs to the technical field of microorganisms. Fictibacillus macauensis The Pseudomonas australis 94-6Y strain is classified and named as Pseudomonas australis, the strain number is 94-6Y, the preservation number is CGMCC No.32718, the strain is a gram-positive strain, the colony is milky white, nearly round, opaque, the surface is slightly rough, and has no luster. The Pseudomonas australis 94-6Y strain can significantly inhibit the water production breeding pathogenic bacteria Vibrio vulnificus, Vibrio splendidus and Vibrio alginolyticus; meanwhile, it is proved that the Pseudomonas australis 94-6Y strain has the ability of efficiently reducing ammonia nitrogen, nitrite nitrogen and chemical oxygen demand in breeding water, is helpful to reduce the breeding disease risk coefficient, improves the breeding water environment, and has important significance for strengthening the research and development of microecological preparation products and popularization of the ecological healthy breeding mode.

The invention discloses a bimetallic nano-enzyme as well as a preparation method and application thereof, and relates to the technical field of biological detection. The bimetallic nano-enzyme is a DNA-Ag / Pt bimetallic nano-enzyme material prepared on the basis of a specific DNA template sequence or containing the specific DNA template sequence, and can be combined with a double-emission ratio type silver nano-cluster to be used for double-signal detection of vibrio parahaemolyticus. The bimetallic nano-enzyme has peroxidase-like activity, the enzyme activity efficiency is adjustable, and the bimetallic nano-enzyme can be used for double-signal detection of vibrio parahaemolyticus. Compared with the prior art, the color development absorbance of the bimetallic nano-enzyme provided by the invention is improved by about 15%, the lowest detection limit of a colorimetric detection method is 10 CFU / mL, and the lowest detection limit of a fluorescence detection method is 1 CFU / mL; the method has no cross reaction on staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, salmonella, vibrio vulnificus and the like, and is suitable for on-site rapid screening, visual interpretation and semi-quantitative detection of scenes such as water samples and marine products.

The application discloses a specific target point for detecting Vibrio vulnificus, a primer, a detection method and application, and belongs to the technical field of food safety detection. The new specific target point for detecting Vibrio vulnificus is screened by establishing a local database and an online comparison method. A double digital PCR method is established by using the new target point and a traditional target point. The PCR primer probe is designed according to the self-screening target gene AOT11_08030 (vv08030) and the traditional target gene vvhA. In order to ensure the specificity and sensitivity of the method, the PCR amplification conditions and the reaction system are reasonably optimized. The experimental results show that the method has the characteristics of high specificity, high sensitivity and simple result determination. The detection process does not need to be opened, and the tedious electrophoresis operation is omitted. The detection time is shortened to 3 hours or less, and the method is suitable for rapid quantitative detection of Vibrio vulnificus in food.