31 results about "Vibrio vulnificus" patented technology

The invention relates to a circular DNA probe for recognizing vibrio vulnificus and a preparation method of the circular DNA probe, specifically, a linear probe carrying DNAzyme is mixed with a looping primer, the mixture is connected under the action of T4DNA ligase to form the circular DNA probe, the circular DNA probe can be specifically recognized with a target and then is subjected to rolling circle amplification to form long-chain DNA, the long-chain DNA contains a large amount of DNAzyme formed by amplification and repeated GC-rich regions, and the long-chain DNA can be used for detecting the vibrio vulnificus. Intramolecular or intermolecular hydrogen bonds are spontaneously formed and are self-assembled to form the DNA hydrogel in a porous state, so that the structure promotes region limitation, and the detection sensitivity is greatly improved; target concentration gradient verification shows that the linear relation of the kit is good, R2 is equal to 0.9915, clinical sample verification shows that the specificity of the kit is high, and the kit can be used for visual and portable detection of vibrio vulnificus.

This invention relates to the field of food testing technology, and more particularly to a colorimetric LAMP method for the simultaneous visual detection of Vibrio vulnificus and Vibrio alginolyticus. The method employs a LAMP detection system composed of DNA-functionalized gold nanospheres and gold nanorods for detecting Vibrio vulnificus and Vibrio alginolyticus, respectively, and LAMP primer pairs. This allows for the detection and determination of the following states of Vibrio vulnificus and Vibrio alginolyticus: when neither pathogen is present, the solution is transparent; when both pathogens are present, the solution is purplish-red; when only Vibrio alginolyticus is present, the solution is gray; and when only Vibrio vulnificus is present, the solution is purple. This method can accurately determine the presence or absence of Vibrio vulnificus and Vibrio alginolyticus, and the specific type of bacteria, avoiding misjudgments, missed detections, and inaccurate traceability of these two types of pathogen contamination in food safety monitoring, clinical practice, and aquaculture protection.

The invention provides a primer combination for a multiple detection kit for marine pathogenic bacteria and the multiple detection kit. The primer combination comprises primer pairs and probes which are used for detecting 13 marine pathogenic bacteria: vibrio alginolyticus, vibrio parahaemolyticus, vibrio cholerae, vibrio vulnificus, vibrio marine fish, vibrio river, vibrio menivibrio, vibrio mimicus, vibrio harveyi, vibrio occultus, vibrio horii, plesiomonas and aeromonas, and the sequence of the primer combination is as shown in SEQ1-SEQ39. According to the present invention, the genomic DNA of 13 marine pathogenic bacteria is identified and detected in one digital PCR reaction through the ultra-multiple digital PCR fluorescence coding technology, and the kit not only covers the common vibrio species such as vibrio cholerae, vibrio vulnificus, vibrio parahaemolyticus and the like, but also can identify the sea area vibrio, vibrio holii, vibrio occultus and the like, and the kit has good sensitivity, specificity and accuracy.

The present invention discloses specific molecular targets of Vibrio vulnificus and a detection method thereof. The nucleotide sequences of the specific molecular targets are as shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 or SEQ ID NO.4. The present invention not only discloses four specific molecular targets for identifying Vibrio vulnificus, but also provides related specific primer sequences and corresponding PCR detection methods. The present invention does not require physiological and biochemical identification, and has the advantages of short detection time, low cost, strong specificity, etc.

The invention discloses a method for preventing and controlling aphids in a seedling stage by using predatory mites, which comprises the following steps of: inoculating latheelaelalaelaps wholei serving as the predatory mites on crop seedlings to prevent and control aphid harm. The invention finds and verifies that the latheelaelalaelaps wholei has obvious predation and control effects on the aphids in the seedling stage for the first time, provides a brand-new and efficient biocontrol natural enemy resource for biological control of the aphids, has important ecological and economic values, is suitable for prevention and control of the aphids in the seedling stage of various crops, and has good popularization and application prospects.

The invention discloses a modified chitosan compound with a function of promoting healing of seawater soaking wounds. The modified chitosan compound DGCS prepared by the invention has good biological activity, has a remarkable promotion effect on cell proliferation, and is enhanced along with the increase of concentration; the inflammation level in cells can be reduced; the healing of a seawater soaking wound can be promoted. An antibacterial experiment shows that the DGCS has good antibacterial activity on escherichia coli, bacillus subtilis, staphylococcus aureus, pseudomonas aeruginosa, enterococcus faecalis, vibrio vulnificus and vibrio parahaemolyticus.

The application provides a new bacteriophage for preventing and treating vibrio diseases of aquatic animals and application thereof, the bacteriophage is preserved in China Center for Type Culture Collection on September 4, 2020, and the preservation number is CCTCC M 2020465. The bacteriophage is a new species of bacteriophage of the Caudovirales class and Queuovirinae subfamily, the whole genome of the bacteriophage is OP331229.1 in the NCBI official website GeneBank. The bacteriophage has a wide phage spectrum, can crack 6 different species and genera of vibrio at the same time, can crack 45 strains of Vibrio parahaemolyticus, 22 strains of Vibrio harveyi, 17 strains of Vibrio alginolyticus, 13 strains of Vibrio vulnificus, 11 strains of Vibrio campbellii and 9 strains of Vibrio cholerae at the same time, can more effectively solve the problem of multiple bacterial disease infections in aquaculture, and can be used as a microecological preparation for preventing and treating vibrio diseases such as Vibrio parahaemolyticus, Vibrio harveyi and Vibrio alginolyticus of aquatic animals.

The present invention belongs to the field of marine biotechnology, and specifically relates to the application of a gene cluster in the rapid detection of Vibrio vulnificus and a primer probe set thereof. The primers are a set of primers corresponding to three gene loci, consisting of upstream and downstream primers and a probe primer; the upstream primer is shown in SEQ ID NO. 1; the downstream primer is shown in SEQ ID NO. 2; and the probe primer is shown in SEQ ID NO. 3. The present invention is aimed at the current detection of Vibrio vulnificus ( V. vulnificus ), corresponding RPA amplification detection primers were designed based on the three specific gene loci obtained through screening, and a strip RPA detection system and method for Vibrio vulnificus was established. This technology is of great value for on-site rapid screening and detection of Vibrio vulnificus in aquaculture, import and export trade, and bathing beaches, and has broad market application prospects.

The invention discloses a broad-spectrum antibacterial lactobacillus plantarum, and belongs to the technical field of microorganisms. The lactobacillus plantarum 9-6 is separated from animal feed, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32568, the lactobacillus plantarum 9-6 and fermentation liquor thereof have an obvious inhibition effect on common vibrio parahaemolyticus, vibrio alginolyticus, vibrio harveyi and vibrio vulnificus, and the lactobacillus plantarum 9-6 and the fermentation liquor thereof have the advantages that the lactobacillus plantarum 9-6 and the fermentation liquor thereof have the obvious inhibition effect; and in addition, the antibacterial agent has a relatively good antibacterial effect on common staphylococcus aureus, escherichia coli, listeria monocytogenes, salmonella enterica and bacillus cereus. The lactobacillus plantarum 9-6 has probiotic safety, can grow under an initial pH condition of 4-10 and a high-salt environment, and plays a role in bacteriostasis under an acidic condition. The invention lays a foundation for developing a good antibacterial probiotic preparation and application of a fermentation culture thereof.

The disclosure provides a chemical synthesis method for a disaccharide fragment of a Vibrio vulnificus biotype 2 serovar A O-antigen, belonging to the chemical field. The disclosure utilizes D-glucose and L-galactose as raw materials to prepare two types of glycosylated building blocks, and designs an efficient synthesis route for constructing the disaccharide fragment. By optimizing protecting groups and optimizing the time of introducing modifying groups, the preparation of the target disaccharide is successfully completed. The raw materials for preparing the disaccharide in the disclosure are readily available, the preparation method is simple and easy to repeat, and will have a good application prospect in the development of new drugs and vaccines for V. vulnificus.

Described herein are compositions, methods, and kits for detecting diarrhea causing pathogens from patient, food, or environmental samples. One embodiment described herein is primer pairs and probes for multiplex polymerase chain reaction (PCR) based assays for the detection of diarrhea causing pathogens, such as Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, Plesiomonas shigelloides, Yersinia enterocolitica, or Aeromonas hydrophila. Other embodiments include methods and kits for detecting diarrhea causing pathogens.

The invention provides application of isoquinoline alkaloid in macleaya cordata to preparation of a product for preventing and treating bacterial pathogenic bacteria of aquatic animals, and relates to the field of macleaya cordata extracts. The alkaloid in the macleaya cordata is used for preparing a product for preventing and / or treating related diseases caused by bacterial pathogenic bacteria of aquatic animals; the alkaloid is sanguinarine and / or chelerythrine; the aquatic animal bacterial pathogenic bacteria are at least one of vibrio harveyi, vibrio vulnificus, vibrio mimicus and vibrio aerogenes. Through tests, alkaloids (sanguinarine and chelerythrine) in macleaya cordata show excellent antibacterial activity on the aquatic animal bacterial pathogenic bacteria, and can be effectively applied to preparation of related products aiming at related diseases caused by the aquatic animal bacterial pathogenic bacteria; the isoquinoline alkaloid in macleaya cordata has the advantages of being green, efficient, low in toxicity, low in residue and not prone to generating resistance.

This invention discloses a quadruple Taqman qPCR reagent for detecting foodborne pathogens in aquatic products. The foodborne pathogens are Vibrio parahaemolyticus, Vibrio vulnificus, Shigella, and Staphylococcus aureus. The reagent includes specific primers and Taqman probes corresponding to the four foodborne pathogens. This invention provides a quadruple Taqman qPCR reagent with high efficiency, accuracy, sensitivity, and speed, offering an accurate, rapid, efficient, and economical method for detecting the above four foodborne pathogens in the quality and safety testing of aquatic products and their processed products. This is of great significance for ensuring the safety of aquatic products for consumption in my country.

The invention relates to the technical field of biological medicines, in particular to application of EGCG (epigallocatechin gallate) and a preparation thereof in inhibiting vibrio vulnificus or diseases caused by the vibrio vulnificus. It is found for the first time that EGCG can inhibit growth of vibrio vulnificus, destroy cell membranes of the vibrio vulnificus and inhibit formation of mycoderm, meanwhile, TLR4, MyD88 and / or NF-kappa B signal channels can be adjusted, host inflammatory response and organ dysfunction are relieved, and therefore EGCG can be used for preventing and / or treating vibrio vulnificus sepsis caused by the vibrio vulnificus; the blank of the existing treatment means in the aspect of anti-vibrio vulnificus and immune double-target intervention is filled, and important theoretical significance and application prospects are achieved.

The application belongs to the technical field of antibacterial peptides, and discloses a novel antibacterial peptide for marine pathogen infection and application thereof. The application takes Aequorea victoria as a research object, analyzes the differential expression of genes of Aequorea victoria after bacterial infection by means of multi-omics technology, predicts candidate antibacterial peptides by using an AI model, verifies the activity by determining the MIC of the polypeptide on bacteria, and thus obtains a high-activity Aequorea victoria antibacterial peptide. The in-vitro activity of the antibacterial peptide is further explored. Then, the hemolytic activity, cytotoxicity and stability of the antibacterial peptide are determined, and the antibacterial mechanism thereof is preliminarily studied. The safety and antibacterial activity of the antibacterial peptide in vivo are further explored through an animal model of Vibrio vulnificus peritoneal infection. The obtained antibacterial peptide 21546, after modification and optimization, is likely to become a powerful lead peptide for developing a novel antibacterial drug for marine pathogen infection, and provides a novel candidate drug for treating secondary infection of marine biological injury, sea combat injury and clinical pathogenic bacteria infection.

The present invention discloses a Vibrio vulnificus selective culture medium and a preparation method thereof. The culture medium comprises a basal culture medium and additives; the basal culture medium comprises peptone, beef extract powder, sodium chloride, potassium chloride, a long-chain alkyl sulfate anionic surfactant or nisin, D-cellobiose, an acid-base indicator, and agar; the additive is tellurite; and the solvent is water. When the basal culture medium contains nisin, the additives further comprise vancomycin, amphotericin B, and polymyxin E. The Vibrio vulnificus selective culture medium of the present invention has high growth selectivity, effectively inhibits the growth of Gram-positive bacteria, effectively inhibits fungal growth, and is sensitive and specific for detecting Vibrio vulnificus. Furthermore, the preparation method does not require autoclaving and avoids the inconvenience of preparing the high-content component, cellobiose, into a sterile additive prior to use, making it more convenient.

The present invention belongs to the field of chemistry. Disclosed in the present invention is a method for the chemical synthesis of a Vibrio vulnificus biotype 2 serotype A O-antigen disaccharide fragment. In the present invention, D-glucose, L-galactose, etc. are used as raw materials to prepare two glycosylated building blocks, and a set of synthesis routes for efficiently constructing a disaccharide fragment is designed. By means of the optimization of a protecting group and the timing for introducing a modification group, the target disaccharide is successfully prepared. The disaccharide prepared in the present invention has easily obtainable raw materials, and a simple and easily repeatable preparation method, and has good application prospects in the aspects such as the development of new drugs and vaccines for Vibrio vulnificus.

The application discloses a Pseudomonas australis 94-6Y strain, a bacterial agent and application thereof, and belongs to the technical field of microorganisms. Fictibacillus macauensis The Pseudomonas australis 94-6Y strain is classified and named as Pseudomonas australis, the strain number is 94-6Y, the preservation number is CGMCC No.32718, the strain is a gram-positive strain, the colony is milky white, nearly round, opaque, the surface is slightly rough, and has no luster. The Pseudomonas australis 94-6Y strain can significantly inhibit the water production breeding pathogenic bacteria Vibrio vulnificus, Vibrio splendidus and Vibrio alginolyticus; meanwhile, it is proved that the Pseudomonas australis 94-6Y strain has the ability of efficiently reducing ammonia nitrogen, nitrite nitrogen and chemical oxygen demand in breeding water, is helpful to reduce the breeding disease risk coefficient, improves the breeding water environment, and has important significance for strengthening the research and development of microecological preparation products and popularization of the ecological healthy breeding mode.

The invention discloses a bimetallic nano-enzyme as well as a preparation method and application thereof, and relates to the technical field of biological detection. The bimetallic nano-enzyme is a DNA-Ag / Pt bimetallic nano-enzyme material prepared on the basis of a specific DNA template sequence or containing the specific DNA template sequence, and can be combined with a double-emission ratio type silver nano-cluster to be used for double-signal detection of vibrio parahaemolyticus. The bimetallic nano-enzyme has peroxidase-like activity, the enzyme activity efficiency is adjustable, and the bimetallic nano-enzyme can be used for double-signal detection of vibrio parahaemolyticus. Compared with the prior art, the color development absorbance of the bimetallic nano-enzyme provided by the invention is improved by about 15%, the lowest detection limit of a colorimetric detection method is 10 CFU / mL, and the lowest detection limit of a fluorescence detection method is 1 CFU / mL; the method has no cross reaction on staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, salmonella, vibrio vulnificus and the like, and is suitable for on-site rapid screening, visual interpretation and semi-quantitative detection of scenes such as water samples and marine products.

The present invention discloses a NAT inhibitor and its application. The present invention conducts virtual screening on the NAT inhibitor derived from Vibrio vulnificus, and obtains the NAT inhibitor, specifically compound AK-968-11563024 or compound AG-205-36710025, which is the first inhibitor targeting (VIBVN) NAT. The present invention also proves through experiments that the two compounds AK-968-11563024 and AG-205-36710025 have good inhibitory effects on the transfer of the acetyl group of acetyl coenzyme A catalyzed by (VIBVN) NAT to 4-acetylsalicylic acid. Among them, the IC50 of compound AK-968-11563024 reaches 16.15 μM. Therefore, the NAT inhibitor has good application value and prospects in the field of drug development targeting (VIBVN) NAT.

The application discloses a marine antibacterial and healing-promoting peptide and application thereof to preparation of a wound infection prevention and treatment product. Through site-directed mutagenesis and other methods, 273 peptide segments with more remarkable antibacterial and healing-promoting activity are preferably obtained. The antibacterial and healing-promoting peptide has broad-spectrum antibacterial activity, can rapidly increase membrane permeability, cause bacterial breakdown, and effectively inhibit growth of gram-negative bacteria such as Escherichia coli, Pseudomonas aeruginosa and Vibrio vulnificus and gram-positive bacteria such as Staphylococcus aureus. The antibacterial and healing-promoting peptide has good biocompatibility, no cytotoxicity, does not cause hemolysis, and can significantly promote cell proliferation and migration. In terms of prevention and treatment of wound infection, the antibacterial and healing-promoting peptide can effectively inhibit bacterial growth, reduce damage of toxins to the body, significantly reduce the level of pro-inflammatory factors, accelerate wound contraction, and improve the wound healing speed and repair quality. The antibacterial and healing-promoting peptide has a wide range of applications and can be applied to drugs and medical biomaterials, and has a very remarkable effect on prevention and treatment of wound infection.

The present invention belongs to the technical field of detection of pathogenic microorganisms, and particularly relates to primers for detecting and identifying Vibrio harveyi and their applications. According to the conserved regions of the virulence genes of Vibrio vulnificus, the present invention designs a primer pair for detecting and identifying Vibrio vulnificus and a kit. The results show that when the primer pair of the present invention or the kit containing the primer pair is used to identify Vibrio vulnificus, it has strong specificity and high sensitivity, and has certain application value in the detection of Vibrio vulnificus in complex water bodies.

The invention belongs to the technical field of antibacterial peptides, and discloses a novel antibacterial peptide aiming at marine pathogen infection and application thereof. The method comprises the following steps: by taking aurelia as a research object, analyzing the differential expression condition of an aurelia gene after bacterial infection by virtue of a multi-omics technology, predicting candidate antibacterial peptides by virtue of an AI model, and carrying out activity verification on MIC of bacteria by measuring polypeptides, so as to obtain the high-activity aurelia antibacterial peptide. The in-vitro activity of the antibacterial peptide is deeply explored. And then determining the hemolytic activity, cytotoxicity and stability, and carrying out preliminary research on the antibacterial mechanism. The safety and antibacterial activity of the antibacterial peptide in vivo are further explored through a vibrio vulnificus abdominal cavity infected animal model. The obtained antibacterial peptide 21546 can possibly become a powerful leading peptide for developing a novel antibacterial drug aiming at marine pathogen infection after being modified and optimized, and a novel candidate drug is provided for treating marine organism injury, marine battle injury secondary infection and clinical pathogenic bacterium infection.

The application discloses application of chebulic acid or chebulinic acid in preparation of a medicine against aquatic pathogenic bacteria. The active tracking method is used to separate, purify and identify the active components in the extract of Terminalia chebula against Vibrio pathogenic bacteria, and the active components are identified as chebulic acid and chebulinic acid. The chebulic acid and the chebulinic acid are simple to prepare, have small toxic and side effects, and have strong bacteriostatic effects, especially on Vibrio parahaemolyticus. The experimental results show that the 96h half lethal concentrations of the chebulic acid and the chebulinic acid on fish are 561.653mg / L and 525.011mg / L respectively, which are 22.47 and 21.00 times of the minimum bacteriostatic concentration 25mg / L of the chebulic acid and the chebulinic acid on Vibrio parahaemolyticus, and the chebulic acid and the chebulinic acid are safe to fish and have broad-spectrum bacteriostatic effects on Pseudomonas aeruginosa, Aeromonas veronii, Aeromonas hydrophila, Vibrio alginolyticus, Vibrio vulnificus and Vibrio parahaemolyticus, and have good application prospects in the field of new environment-friendly fish medicines.

The application discloses a specific target point for detecting Vibrio vulnificus, a primer, a detection method and application, and belongs to the technical field of food safety detection. The new specific target point for detecting Vibrio vulnificus is screened by establishing a local database and an online comparison method. A double digital PCR method is established by using the new target point and a traditional target point. The PCR primer probe is designed according to the self-screening target gene AOT11_08030 (vv08030) and the traditional target gene vvhA. In order to ensure the specificity and sensitivity of the method, the PCR amplification conditions and the reaction system are reasonably optimized. The experimental results show that the method has the characteristics of high specificity, high sensitivity and simple result determination. The detection process does not need to be opened, and the tedious electrophoresis operation is omitted. The detection time is shortened to 3 hours or less, and the method is suitable for rapid quantitative detection of Vibrio vulnificus in food.

The application discloses a method for specifically detecting Vibrio vulnificus and application. The application takes the recA gene of Vibrio vulnificus as a target, designs a primer probe group capable of specifically detecting Vibrio vulnificus, and the detection sensitivity can reach 0.01 fg / ul. In addition, the application can distinguish Vibrio vulnificus from its similar species, Vibrio tamarinicus and Vibrio anguillarum, in the recA gene, has good specificity and repeatability. The application can provide an effective technical means for disease detection and monitoring of Vibrio vulnificus in the process of aquatic animal breeding, and has a wide application prospect in aquaculture.

The invention discloses a strain of Australian pseudobacillus 94-6Y as well as a microbial agent and application thereof, and belongs to the technical field of microorganisms. The invention provides a strain of pseudomonas macaurae 94-6Y, which is classified and named as pseudomonas macaurae, the strain number is 94-6Y, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32718, the strain is a gram-positive strain, and the bacterial colony is milk white, nearly round, opaque, slightly rough in surface and matte. The pseudomonas australis 94-6Y is capable of remarkably inhibiting aquaculture pathogenic bacteria vibrio vulnificus, vibrio splendidus and vibrio alginolyticus; meanwhile, the invention also proves that the pseudobacillus Australiae 94-6Y has the capability of efficiently reducing ammonia nitrogen, nitrite nitrogen and chemical oxygen demand of the culture water body, is beneficial to reducing the risk coefficient of culture diseases and improving the environment of the culture water body, and has important significance on enhancing the research and development of microecological preparation products and popularizing an ecological healthy culture mode.

The invention belongs to the technical field of marine organisms, and particularly relates to application of a gene cluster in rapid detection of vibrio vulnificus and a detection primer probe set of the gene cluster. The primer is a complete set of primer combination corresponding to three gene loci, and consists of upstream and downstream primers and a probe primer; the upstream primer is as shown in SEQ ID NO. 1; the downstream primer is as shown in SEQ ID NO. 2; and the probe primer is as shown in SEQ ID NO. 3. Aiming at the current vibrio vulnificus, a corresponding RPA amplification detection primer is designed according to three specific gene loci obtained through screening, a test strip RPA detection system and method of the vibrio vulnificus are established, and the technology has an important value for on-site rapid screening and detection of the vibrio vulnificus in aquatic product culture, import and export trade and bathing beach, and has a wide application prospect. The market application prospect is wide.

The invention relates to a rapid vibrio parahaemolyticus detection kit based on a one-tube method RPA-CRISPR / Cas12a and a detection method of the rapid vibrio parahaemolyticus detection kit. A specific RPA (recombinase polymerase amplification) primer and CRISPR crRNA (clustered regularly interspaced short palindromic repeats) are designed by taking a ToxR gene of vibrio parahaemolyticus as According to the established one-tube method RPA-CRISPR / Cas12a detection system, the risk of aerosol pollution caused by a cover opening process is effectively avoided. The method disclosed by the invention is high in specificity, and has no cross reaction with related vibrios such as vibrio vulnificus, vibrio alginolyticus, vibrio cholerae, vibrio harveyi and vibrio anguillarum, and other aquatic pathogenic bacteria such as pseudomonas aeruginosa and pseudomonas plecoglossicida; the sensitivity is high, and the lowest detection limit of genome DNA is as low as 1 pg / mu L; the detection is rapid, and the whole process of nucleic acid amplification and fluorescence detection only needs about 30 minutes. The kit and the method do not need complex instruments and equipment, are simple to operate, are suitable for on-site rapid detection of aquatic product samples in a base layer, and have important application values in early screening, epidemiological investigation and comprehensive prevention and control of vibrio parahaemolyticus.

The invention discloses a preparation method and application of a prawn complete expanded feed rich in vibrio bacteriophage. The invention relates to a preparation method and application of a prawn complete expanded feed rich in vibrio bacteriophage. The expanded feed comprises soybean meal, rapeseed meal, cottonseed meal, fish meal, flour, fish meal, monocalcium phosphate, vitamin A + E and various probiotics such as bacillus subtilis, lactic acid bacteria, saccharomyces cerevisiae, enterococcus faecalis and clostridium butyricum. In addition, the compound bacteriophage comprises a vibrio parahaemolyticus bacteriophage, a vibrio alginolyticus bacteriophage, a vibrio harveyi bacteriophage and a The expanded feed is rich in nutrition and probiotics, can effectively promote the growth of prawns and prevent the explosive growth of vibrios, and has broad spectrum, high efficiency, safety and sustainability on the prevention and control of vibrios.