Colorimetric LAMP method for simultaneous visual detection of vibrio vulnificus and vibrio alginolyticus

By using a LAMP detection system composed of DNA-functionalized gold nanospheres and gold nanorods, combined with a machine learning model, we have achieved accurate detection of multiple states of Vibrio vulnificus and Vibrio alginolyticus. This solves the problem that existing technologies cannot accurately distinguish the combined states of pathogens and has the ability to detect them quickly and with high precision.

CN122279067APending Publication Date: 2026-06-26OCEAN UNIV OF CHINA
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Patent Information

Application Number
CN202610607074.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-06
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

Existing LAMP detection methods cannot achieve simultaneous visual detection of Vibrio vulnificus and Vibrio alginolyticus, especially in terms of accurately distinguishing the presence of different combinations of pathogens, and are subject to cross-reaction interference, leading to misjudgment and missed detection.

Method used

A LAMP detection system composed of DNA-functionalized gold nanospheres and gold nanorods was used to modify probes for Vibrio vulnificus and Vibrio alginolyticus, respectively. The presence of pathogens in the sample was determined by color changes, and automatic interpretation was performed by combining machine learning models.

Benefits of technology

It enables accurate detection and differentiation of Vibrio vulnificus and Vibrio alginolyticus in multiple states, avoiding misjudgment and missed detection. The detection limit is 102 CFU/mL, and it is rapid and highly reproducible.

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Abstract

The invention relates to the technical field of food detection, in particular to a colorimetric LAMP method for simultaneous visual detection of vibrio vulnificus and vibrio alginolyticus. According to the method, DNA (Deoxyribose Nucleic Acid) functionalized gold nanospheres and gold nanorods for detecting vibrio vulnificus and vibrio alginolyticus respectively and an LAMP primer pair are adopted to form an LAMP detection system, so that the following states of the vibrio vulnificus and the vibrio alginolyticus are detected and judged: when no two pathogenic bacteria exist in a solution, the solution is transparent; when two pathogenic bacteria exist at the same time, the solution is purplish red; when only vibrio alginolyticus exists, the solution is gray; when only vibrio vulnificus exists, the solution is purple. The method can accurately judge whether vibrio vulnificus and vibrio alginolyticus exist or not, particularly what kind of vibrio vulnificus and vibrio alginolyticus exist and other situations, and avoids the situations of misjudgment, missed judgment and incapability of accurately tracing to the source of pollution of the two kinds of pathogenic bacteria in food safety monitoring, clinical and culture protection.
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