11 results about "Gold nanospheres" patented technology

This invention relates to the field of food testing technology, and more particularly to a colorimetric LAMP method for the simultaneous visual detection of Vibrio vulnificus and Vibrio alginolyticus. The method employs a LAMP detection system composed of DNA-functionalized gold nanospheres and gold nanorods for detecting Vibrio vulnificus and Vibrio alginolyticus, respectively, and LAMP primer pairs. This allows for the detection and determination of the following states of Vibrio vulnificus and Vibrio alginolyticus: when neither pathogen is present, the solution is transparent; when both pathogens are present, the solution is purplish-red; when only Vibrio alginolyticus is present, the solution is gray; and when only Vibrio vulnificus is present, the solution is purple. This method can accurately determine the presence or absence of Vibrio vulnificus and Vibrio alginolyticus, and the specific type of bacteria, avoiding misjudgments, missed detections, and inaccurate traceability of these two types of pathogen contamination in food safety monitoring, clinical practice, and aquaculture protection.

The invention belongs to the field of preparation of organic-inorganic hybrid materials, and relates to a preparation method and application of cucurbit [n] uril-gold nanoparticles. Through a simple one-pot synthesis method, chloroauric acid, silver nitrate, a reducing agent and cucurbit [n] uril are added for co-reduction to prepare hollow gold nanoflowers; the gold nanospheres with different sizes are prepared by regulating and controlling the input amount of cucurbit [n] uril. The gold nanoparticle hybrid material shows excellent hydrogen evolution side reaction inhibition capability in the application of electro-catalysis CO2 reduction, and the CO selectivity is obviously improved.

This invention discloses an internal standard-assisted petal-shaped SERS nanoprobe, its preparation method, and its applications. The internal standard-assisted petal-shaped SERS nanoprobe of this invention is a core-molecule-shell structure gold nanoprobe consisting of a gold nanosphere core, an internal standard modifying molecule, a petal-shaped gold nanoshell, and a recognition molecule, 4-nitrobenzenethiophenol. The internal standard modifying molecule is a Raman signal molecule with nitrile, alkyne, or azide functional groups. This invention uses a Raman signal molecule as an internal standard modifying molecule to modify the gold nanosphere core. The corrected working curve exhibits good reproducibility and small error, improving the reliability of the detection results. Growing a petal-shaped gold nanoshell on the molecular layer of the internal standard modifying molecule enhances the SERS activity both inside and outside the SERS substrate, and the petal-shaped shell increases the surface area of ​​the SERS substrate. Furthermore, the recognition molecule of this invention enables ratiometric Raman detection of hydrogen sulfide, effectively reducing background signal interference and improving detection sensitivity.

This invention provides peony-shaped gold nanoparticles with an uneven core-shell structure, their preparation method, and applications. We first prepare 50 nm diameter gold nanospheres, then add HAuCl₄, and perform centrifugation and stirring to finally obtain the target product. This structure significantly improves surface roughness and increases the distribution density of electromagnetic "hot spots" while ensuring good particle stability, thereby enhancing the sensitivity of the SERS signal.

The application discloses a flexible enzyme glucose sensor capable of in-vitro detection, which comprises a flexible substrate and an electrode system arranged on the flexible substrate, the electrode system comprising a working electrode, a counter electrode and a reference electrode, a functional layer formed by compounding amino-graphene quantum dots and hollow gold nanospheres is modified on the working electrode, and glucose oxidase immobilized by chitosan is further modified on the functional layer. The sensor has a good linear relationship in the range of 50 muM to 1.4 mM, has the advantages of high sensitivity and good stability, and can be used for detecting urine glucose.

The application discloses a method for detecting benzaldehyde by using a three-dimensional hot spot SERS substrate, and belongs to the field of nanomaterials and biosensing technology, and the method comprises the following steps: detecting benzaldehyde in a to-be-detected sample by using a three-dimensional hot spot SERS substrate, and determining whether the to-be-detected sample contains benzaldehyde or the content of benzaldehyde in the to-be-detected sample by the change of a Raman characteristic peak before and after detection; the structure of the three-dimensional hot spot SERS substrate comprises a substrate and a gold nanosphere superlattice two-dimensional film attached to the substrate, the gold nanosphere superlattice two-dimensional film is modified with a beta-cyclodextrin-p-aminophenylthiophenol inclusion compound and loaded with gold@gold silver hollow nanorods; the SERS substrate has a three-dimensional hot spot superlattice-hollow rod structure. The method has low detection limit, high selectivity and specificity, and lays a foundation for realizing a high-performance and customizable sensing platform.

The application discloses an engineered hepatocyte micro-nanorobot and a preparation method and application thereof, and the preparation method comprises the following steps: adding gold nanosphere dispersion liquid drop by drop into isopropyl alcohol, then adding 4-mercapto benzoic acid and polyacrylic acid in sequence and performing reaction, adding tetraethyl orthosilicate and ammonia water and performing reaction, obtaining Au-SiO2 nanoparticles and performing reaction with chloroplatinic acid and ascorbic acid to obtain an intermediate, and performing etching on the intermediate to obtain AP nanorobots; the enucleated hepatocytes expressing CXCR4 are co-incubated with the AP nanorobots functionalized by DSPE-Hyd-PEG-SH polymer; the engineered hepatocyte micro-nanorobot is based on the synergistic mechanism of cell metabolism regulation-nano-physical sensitization, can overcome the radiotherapy resistance of solid tumors, combines specific chemotactic characteristics with radiotherapy, can be targeted to accumulate in tumor tissues, and can effectively reverse the metabolic-driven radiotherapy resistance barrier.

The invention discloses a preparation method of a spherical DNA nano machine for detecting O6-methylguanine-DNA methyltransferase (MGMT) and application of the spherical DNA nano machine in detection of the expression level of tumor cells MGMT. The method comprises the following steps: firstly, designing a DNA walker with MGMT recognition and signal amplification functions, and fixing the DNA walker on the surface of a gold nanosphere through sulfydryl modification to construct a spherical DNA nanomachine; when the MGMT interacts with a DNA Walker chain, a methylated basic group G on DNA enzyme can be repaired, so that the activity of the DNA enzyme is recovered, an enzyme digestion reaction is initiated, a Track chain marked with a fluorophore is broken and released, a fluorescence signal change is generated, and high-sensitivity detection of the MGMT is realized. The method can effectively detect the MGMT protein in a low concentration range, and has a good biomedical application prospect.

The invention belongs to the technical field of medical materials, and discloses a targeted oxygen-carrying and drug-carrying hollow gold nanosphere connected with a photosensitizer and a preparation method thereof. The preparation method comprises the following five steps: preparing hollow gold nano-spheres, coupling folic acid, conjugating Ce6, carrying Dox and carrying oxygen, so that the hollow gold nano-particles connected with a photosensitizer Ce6 and folic acid with a targeting effect are successfully loaded with oxygen-containing perfluorohexane and a chemotherapeutic drug doxorubicin. The invention provides an efficient and safe tumor oxygenation strategy, develops a tumor specific targeting nano artificial oxygen carrier, realizes tumor oxygen supply, and solves the problem of poor curative effects of chemotherapy and photodynamic therapy caused by oxygen deficit.

The invention belongs to the field of Raman detection, and particularly relates to a surface-enhanced Raman detection method for amino acid and a detection substrate thereof. According to the amino acid concentration detection method based on the low-frequency Raman scattering combined surface enhancement technology and the detection substrate thereof, the problems of low sensitivity, long consumed time, high cost and the like in the existing amino acid detection technology are effectively solved. By designing surface enhanced Raman scattering nano-particles without low-frequency Raman interference signals, namely double-sphere structure nano-particles composed of gold nano-spheres and ferroferric oxide nano-spheres, and combining with a magnetic aggregation substrate, high-sensitivity and rapid detection of various amino acids is realized.

The invention aims to provide a construction method of a three-dimensional laminated chiral nano-composite structure based on a DNA origami template. The construction method comprises the following steps: carrying out hydrophilic treatment on a silicon substrate by using a plasma cleaning machine; designing and synthesizing a DNA origami template, wherein the DNA origami template is provided with a plurality of symmetrically distributed capture sites; respectively modifying the gold nanodisk and the gold nanosphere by adopting DNA (Deoxyribonucleic Acid); spreading a DNA origami template on the surface of the treated substrate; the method comprises the following steps: hybridizing a DNA-modified gold nanodisk with a template, and fixing the hybridized gold nanodisk at the central position of DNA origami; hybridizing the DNA modified gold nanospheres with a DNA chain on the gold nanodisk to form a three-dimensional laminated structure; and cleaning the uncombined nanoparticles to obtain the target composite structure. According to the method, accurate positioning of the gold nanodiscs and the gold nanospheres in a three-dimensional space and ordered stacking of the gold nanodiscs and the gold nanospheres in the vertical direction are achieved through the DNA origami template, and the chiral nano composite structure with the three-dimensional lamination effect is constructed.