104 results about "Gold nanospheres" patented technology

Provided are novel nanostructures comprising hollow nanospheres and nanotubes for use as chemical sensors and molecular specific photothermal coupling agents. The nanostructures can be used in laser-induced phototherapy for treatment of cancer and other disorders. The nanostructures can also be used as a sensor that detects molecules. The nanostructures are of particular use in the fields of clinical diagnosis, clinical therapy, clinical treatment, and clinical evaluation of various diseases and disorders, manufacture of compositions for use in the treatment of various diseases and disorders, for use in molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof. The hollow gold nanospheres have a unique combination of spherical shape, small size, and strong, tunable, and narrow surface plasmon resonance absorption covering the entire visible to near IR region.

The invention belongs to the technical field of neoplastic disease diagnosis, and discloses a kit for early diagnosis of neoplastic disease, a method and applications of the kit. The kit includes a specific capturing and detecting system for a tumor exosome and a molecular beacon fluorescent detection system. The specific capturing and detecting system for the tumor exosome includes a solution ofa magnetic gold nanosphere composite modified by a tumor exosome specific ligand and adopting ferric ferrous oxide as a core, and a gold nanocage solution modified by the exosome specific ligand. Themolecular beacon fluorescent detection system includes a molecular beacon probe provided with a fluorescent group, fluorescent amplification substrate hybrid chains provided with fluorescent groups and a buffer liquid. The kit and the method combine tumor exosome specific capturing and analysis and a molecular beacon fluorescent detection method, and greatly increases accuracy and reliability of early diagnosis of tumor.

The invention discloses a pH-responsive anti-tumor drug carrier material and a preparation and application of the pH-responsive anti-tumor drug carrier material. The preparation method comprises the following steps that (1) gold nanoparticles wrapped by mesoporous silica are prepared, and the gold nanoparticles comprise gold nanospheres or gold nanorods; (2) gold nanoparticles wrapped by carboxylation mesoporous silica are prepared; (3) water-soluble zinc oxide quantum dots are prepared; and (4) the pH-responsive anti-tumor drug carrier material is prepared. According to the pH-responsive anti-tumor drug carrier material and the preparation and application of the pH-responsive anti-tumor drug carrier material, the whole technological process design of the preparation method, the parameter conditions adopted by the reaction steps and the like are optimized, and the existing problems that drug releasing is uncontrollable, the drug loading capacity is low, and side effects are prone to being caused when the gold nanoparticles wrapped by the mesoporous silica serve as a drug carrier can be effectively solved.

Provided are novel nanostructures comprising hollow nanospheres (HGNs) and nanotubes for use as chemical sensors, molecular specific photothermal coupling agents, and photothermal ablation compounds. The nanostructures can be used in electromagnetic radiation-induced phototherapy for treatment of cancer and other disorders. The nanostructures can also be used as a sensor that detects molecules. The nanostructures are of particular use in the fields of clinical diagnosis, clinical therapy, clinical treatment, and clinical evaluation of various diseases and disorders, manufacture of compositions for use in the treatment of various diseases and disorders, for use in molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof. The hollow gold nanospheres have a unique combination of spherical shape, small size, and strong, tunable, and narrow surface plasmon resonance absorption covering the entire visible to near IR region.

The invention discloses a double-ion response type SERS (Surface Enhanced Raman Scattering) probe and a preparation method thereof. The probe comprises two nano particles with independent core-shell structures, wherein a carrier nano particle comprises four layers of core-shell structures; the four layers of core-shell structures are as follows: the innermost layer is made of Fe3O4 magnetic nano balls, a layer of silicon dioxide is coated outside the innermost layer, the second-outer layer is a gold foil shell layer, and the outermost layer is an oligonucleotide coating layer; an object nano particle comprises two layers of core-shell structure; the two layers of core-shell structure are as follows: the inner core is made of Raman molecule marked gold nano-balls, and a shell is an oligonucleotide coating layer. Due to the adoption of a specific base sequence in the oligonucleotide coating layer, the identify and SERS trace detection on two metal ions, namely silver/mercury, in an environment solution can be performed at the same time; furthermore, under the action of an external magnetic field, the probe and the obtained silver/mercury ions can be rapidly separated from an initial environment solution system, and therefore, the function of purifying the environment is realized.

The invention discloses a homogeneous phase multi-component immunoassay method based on a surface plasma coupling effect. A gold nanosphere probe (Raman reactive dyes and capture antibodies are embellished on the surface of the gold nanosphere probe) and a gold nanorod probe (Raman reactive dyes and detection antibodies are embellished on the surface of the gold nanorod probe) are respectively prepared; the Raman reactive dyes carry sulfydryl self and are free of fluorescent background; and the antibodies are subjected to TCEP (Tris(2-carboxyethyl) phosphine) reduction treatment. The antigen of an object to be tested has immune sandwich reaction with the antibodies so as to generate an immune complex, so that the distances between nano particles are shortened, the surface plasma coupling effect is generated, the Raman signals of the surface embellishing dyes on the surfaces of the nano particles are significantly enhanced, and the SERS (Surface Enhanced Raman Scattering) quantitative analysis of the antigen is realized by using the coupling enhancing effect. The method is simple and rapid, the detection process needs only one step of operation without repeated elution or separating samples, the synchronous quantitative analysis to a plurality of components in the samples is realized with high sensitivity and good uniqueness, and thus the method is expected to become a new method in proteomics research and drug therapeutic effect evaluation.

A near-infrared mediated drug delivery system comprising a plurality of microspheres made of polymeric material, each sphere containing a plurality of hollow gold nanospheres together with drug product, wherein upon NIR irradiation, the drug product is released from the microsphere.

The invention discloses a penetration-enhanced gold nanocluster drug-loaded targeting preparation and a preparation method and application thereof. The targeting preparation comprises gold nanoclusters, a cross-linking agent and a tumor treatment drug, wherein the gold nanoclusters are obtained by assembling gold nanospheres with the cross-linking agent, and the surface of the gold nanoclusters ismodified with the targeting tumor treatment drug of human epidermal growth factor receptor-2. The preparation can be used as an antitumor drug carrier, specifically targets to a tumor, enables the drug to be efficiently released at a tumor site through tumor microenvironment reduction and NIR irradiation, and improves the treatment effect in combination with thermal therapy and action. By the preparation, the advantages of gold in imaging aspects such as CT imaging and photoacoustic imaging (PA) can also be combined for medical diagnosis, and a purpose of multi-mode accurate tumor diagnosis and treatment is achieved.

The invention discloses a porous gold-silver alloy nanomaterial as well as a preparation method and an application thereof. The material is a hexagonal porous gold-silver alloy nano-sphere ordered array which is not arranged closely, and the period of the material ranges from 350 nm to 750 nm. The preparation method of the material comprises the steps as follows: a single-layer colloidal microsphere array is used as a formwork, a layer of gold film is deposited on the surface of the formwork with a physical deposition method, then heat treatment is performed, and a gold nano-sphere ordered array is prepared; a layer of silver film is deposited on the surface of the gold nano-sphere ordered array with a physical deposition method, then heating and annealing treatment is performed, and a gold-silver alloy nano-sphere ordered array is prepared; chemical corrosion treatment is performed on the gold-silver alloy nano-sphere ordered array, and thus the porous gold-silver alloy nanomaterial is prepared. With the method, the large-area hexagonal porous gold-silver alloy nano-sphere ordered array which is not arranged closely and is controllable in period and size can be prepared, capping agents and stabilizing agents are not required, and the array can be adsorbed by target molecules easily and is convenient to reuse.

The invention discloses a monodisperse hollow gold nanosphere which is provided with a monodisperse hollow kernel and a monodisperse gold nano outer layer, wherein the monodisperse gold nano outer layer is one or more of a gold nano layer, a silver nano layer, a copper nano layer and a platinum nano layer; the monodisperse hollow kernel is formed by eroding a monodisperse organic or inorganic nanosphere. Compared with a conventional hollow gold nanosphere, the hollow gold nanosphere disclosed by the invention is high in monodispersity. Therefore, an SERS (surface enhanced raman scattering) probe prepared by the hollow gold nanosphere with high monodispersity has immunoassay characteristics of extremely high uniformity, sensitivity and repetitiveness. High-precision quantitative detection is facilitated during quantitative analysis of biological markers.

The invention provides a 2-(9-methylene anthracene) thiosemicarbazide and TAT modified gold nanoparticle drug delivery system. The 2-(9-methylene anthracene) thiosemicarbazide and TAT modified gold nanoparticle drug delivery system has the advantages that a gold nanosphere drug delivery system with the particle size being 3.8nm (sodium borohydride reduction method) and a gold nanosphere drug delivery system with the particle size being 22.1nm (sodium citrate reduction method) are built, the surfaces of the systems are modified with polypeptide TAT, the systems modified by the polypeptide TAT are used as the carriers of 2-(9-methylene anthracene) thiosemicarbazide, the fact that the drug-resistance index of large-particle-size gold nanospheres to drug-resistance cells is smaller is discovered through the antitumor activity evaluation of MCF-7 sensitive cells and MCF-7/ADR drug-resistance cells, and the fact shows that large-particle-size carriers are more suitable for tumor drug resistance treatment and the 22.1nm gold nanospheres have an enhancing effect on the Raman signal of the 2-(9-methylene anthracene) thiosemicarbazide and can be used for cellular-level Raman imaging.

The invention relates to an LSPR adjustable gold@silver core-shell nanostar and a preparation method thereof. The gold@silver core-shell nanostar comprises a gold nanostar and a silver shell layer coated on the surface of the gold nanostar, wherein the gold nanostar comprises a gold nanosphere positioned at the core and gold nanospines on the surface of the gold nanosphere. Firstly, a liquid-phasechemical method regulated by seeds is adopted, the concentration of the gold nanosphere in a precursor is regulated, and the regulating effect of a surfactant on the growth of a gold nanostructure isutilized, so that the gold nanostar with adjustable size and the LSPR peak between 587-890 nm is prepared; and a polyhydroxy alcohol reduction method is further adopted, ethylene glycol is used as asolvent and a reducing agent, silver nitrate is used as a silver source, the silver shell layer is coated on the surface of the gold nanostar in situ, the surface enhanced Raman scattering (SERS) activity of the nanostar structure is greatly improved, and meanwhile the morphology and the LSPR interval of the core-shell nanostar are maintained. The LSPR adjustable gold@silver core-shell nanostar provides a new choice of materials for a plasmon-driven solar-chemical energy photocatalytic reaction.

The invention relates to a gold nanosphere/melanin composite nanometer opto-acoustic probe with ultra-strong opto-acoustic effect and preparation thereof. The gold nanosphere/melanin composite nanometer opto-acoustic probe is prepared by compounding dodecylamine-modified gold nanospheres with melanin nanometer particles coating the dodecylamine-modified gold nanospheres, wherein a mass ratio of the dodecylamine-modified gold nanospheres to the melanin nanometer particles is 0.1-10: 1. Compared with the prior art, the composite nanometer opto-acoustic probe provided by the invention can realize circulation in the body for a certain period of time and can be disposed at a focus part after completion of opto-acoustic imaging; and the dissociated small-sized gold nanospheres can be removed via metabolism of the kidney and are thus prevented from accumulation in biological tissue and incurrence of damage.

The invention relates to a precious metal-doped titanium dioxide composite material and a preparation method thereof. The preparation method comprises the following steps: S1, preparing a gold nanosphere; S2, coating the surface of the gold nanosphere with a silica coating; S3, coating the surface of an Au-SiO2 core-shell nanometer compound with a titanium dioxide precursor coating; S4, removing aSiO2 core in an Au-SiO2-TiO2 precursor ternary core-shell nanometer compound; and S5, calcining an Au-TiO2 precursor yolk-structured nanometer compound so as to obtain the gold-doped titanium dioxidecomposite material. The preparation method of the invention is simple in process, easy to operate, low in production cost, small in process pollution and suitable for large-scale production; and theprepared precious metal-doped titanium dioxide composite material has high purity, good crystallinity, good monodispersity, uniform particle sizes, high utilization rate of solar energy and excellentphotocatalytic performance.

The invention discloses a preparation method and applications of hollow gold nanospheres modified by CpG oligodeoxynucleotide. Cobalt chloride is subjected to reduction by adopting sodium borohydride,thus cobalt nanoparticles are obtained, cobalt nanoparticles are adopted for carrying out reduction on chloroauric acid, and thus hollow gold nanospheres are prepared; CpG oligodeoxynucleotide, a surfactant and a buffer solution are added into a solution of the hollow gold nanospheres, shaking culture is carried out, and thus the hollow gold nanosphere material modified by CpG oligodeoxynucleotide is obtained. According to the technical scheme, by utilizing the property that the hollow gold nanospheres have the large loading capacity, CpG is loaded through the self-assembly effect, the synthesis method is simple, and the method is suitable for large-scale production and application; the cell uptake capacity of CpG oligodeoxynucleotide can be enhanced, and the biocompatibility is good; thehollow gold nanospheres modified by CpG oligodeoxynucleotide have the near-infrared surface plasma adsorbing effect, the primary tumor is eliminated by utilizing the photothermal ablation effect, a tumor antigen is generated in situ, under the promoting of CpG oligodeoxynucleotide, the antitumor immunity of the whole body is induced, and the distant metastasis tumor is further treated.

The invention discloses a rapid on-site detection method for trichlorfon and simazine in seawater. The method comprises the following steps: (1) synthesizing gold nanorods by a seeding method; (2) adding L-cysteine to a gold nanorod solution to obtain an L-cysteine -modified gold nanorod material; (3) hydrothermally synthesizing gold nanospheres; (4) adding p-aminothiophenol to gold nanospheres toobtain a p-aminothiophenol-modified gold nanosphere composite; (5) mixing the L-cysteine -modified gold nanorod material with the p-aminothiophenol-modified gold nanosphere composite to obtain a goldnanorod and gold nanosphere composite as a SERS active substrate by peptide bonding; and (6) detecting the trichlorfon and the simazine by a Raman spectrometer to achieve qualitative and quantitativeanalysis of the trichlorfon and the simazine in the seawater. The rapid on-site detection method has the characteristics such as rapid analysis, high sensitivity, small sample consumption, wide application range, simple operation and convenient carrying.

The invention discloses a method for synthesizing a gold nanosphere, a gold nanorod and a gold nanowire in an organic phase. The method comprises the steps that a gold precursor is dissolved in an organic solvent, then a solutizer and ultrasonic are added, followingly, a reducing agent is added, stirring reaction is performed for 2-6h under the room temperature, and a gold nano material is obtained. According to the method, oleylamine/oleic acid is introduced into a solutizing step of the gold precursor, the gold precursor is allowed to have good dispersity in the organic solvent, the oleylamine/oleic acid is selectively adsorbed to the surface of the gold nano material, the gold nanosphere, the gold nanorod and the gold nanowire are synthesized, and by adjusting the proportion of the goldprecursor to the reducing agent to the solutizer easily, the grain size of the gold nanosphere and the shape of the gold nano material can be effectively controlled; and the method provided by the invention is simple and easy to implement, good in repeatability, high in stability and high in yield.

The invention discloses a probe using gold nanospheres as a carrier and application of the probe to detection of Hg<2+> based on solid-state nanopores, and belongs to the technical field of heavy metal detection. The gold-nanosphere-combined probe is used to identify Hg<2+> through a solid-state nanopore sensor, so that the highly sensitive detection of mercury ions by the solid-state nanopore sensor is realized. The difficulty that ions are directly to detect due to pores are too large in solid nanopore signal recognition is overcome, by taking gold nanoparticles as a carrier to carry out signal amplification, the high sensitive detection of the solid-state nanopores on Hg<2+> is realized, and the application of the solid-state nanopores in a molecular detection field is expanded.

The invention relates to a method for preparing an urchin-like gold nano-crystal with controllable lug spacing. The method comprises the following steps: firstly modifying double-stranded DNA (dsDNA)molecules to the surface of gold nano-spheres (AuNSs) to prepare an AuNSs/dsDNA compound; and performing an in-situ reduction reaction on chloroauric acid and a reducing agent in a solution to generate the urchin-like gold nano-crystal under the guiding effect of the AuNSs/dsDNA compound. The invention further relates to the urchin-like gold nano-crystal prepared by the preparation method, the spacing of lugs on the surface of the nano-crystal and the location of largest resonance absorption peak of plasma on the surface of the nano-crystal can be controlled by changing the composition of dsDNA. Compared with the prior art, the preparation method has the advantages of easy implementation and low synthesis cost, and can regulate and control the surface spacing and performance of the urchin-like gold nano-crystal.

The invention belongs to the field of tumor cytology, and discloses a method for tumor cell detection through porous gold nanospheres. The method comprises the following steps: (1) preparing the porous gold nanospheres; (2) carrying out co-incubation on the porous gold nanospheres and a sulfydryl-modified aptamer to prepare functionalized porous gold nanospheres; (3) binding the functionalized porous gold nanospheres with sample cells, and removing the unbound porous gold nanospheres, so that a cell sample to be detected is obtained; and (4) adding a bicarbonate solution into the cell sample to be detected, and carrying out illumination under a 808nm laser. The method for the tumor cell detection through the porous gold nanospheres has the advantages that the operation is simple, and the detection cost is low; and the adopted porous gold nanospheres can be simply prepared, achieve high light and heat stability and high biocompatibility, and can produce a high temperature under the illumination of the 808nm laser for thermal decomposition of bicarbonate, so that air pressure changes in a reaction system can be monitored to distinguish tumor cells from normal cells.

The invention relates to an Au-TiO2 yolk-structured nanocomposite material and a preparation method thereof. The preparation method comprises the following steps: firstly preparing a gold nanosphere;then coating the surface of the gold nanosphere with a silicon dioxide layer; coating the surface of the silicon dioxide layer with a titanium dioxide precursor mesoporous material layer; then removing the silicon dioxide layer; and finally carrying out hydro-thermal treatment to obtain the Au-TiO2 yolk-structured nanocomposite material with a sheet-like branch structure on the surface. The preparation method of the invention is simple in process, easy to operate, low in production cost, small in process pollution and suitable for large-scale production; and the prepared Au-TiO2 yolk-structured nanocomposite material has a unique movable core and has the sheet-like branch structure on the surface, which can increase the specific surface area of the material, greatly enhance the photocatalytic performance of the material and realize high utilization rate of solar energy.

The invention discloses a Matryoshka doll-type nano gold rod system loaded with di-(1-(4-hydroxyphenyl)-5-sulfydryl-tetrazolyl) silicon phthalocyanine as well as a preparation method and an application thereof. The preparation method comprises the following steps: firstly synthesizing a gold nanorod, then coating the surface of the gold nanorod with aminated silicon dioxide, and finally coating the surface of the gold nanorod with aminated silicon dioxide with a layer of gold nanospheres to prepare the Matryoshka doll-type gold nanorod with a three-layer cavity structure, enabling dichlorosilane phthalocyanine to react with 1-(4-hydroxyphenyl)-5-sulfydryl-tetrazole to synthesize di-(1-(4-hydroxyphenyl)-5-sulfydryl-tetrazole) silicon(IV)phthalocyanine; and finally, preparing a Matryoshka doll-type gold nanorod system loaded with di-(1-(4-hydroxyphenyl)-5-sulfydryl-tetrazolyl)silicon(IV)phthalocyanine and Matryoshka doll-type gold nanorods through a molecular assembly method, and takingthe Matryoshka doll-type gold nanorod system as photodynamic and photothermal therapy combined inactivation drug-resistant bacteria and tumors.

The invention provides an enhanced photodynamic anti-tumor nano-drug loading system which relates to the technical field of anti-tumor drugs. The nano-drug loading system is modified with hydrogen oxide enzyme, dendritic gold nanosphere shells and nano-liposomes sequentially from inside to outside, and the nano-liposomes are coated with chemotherapeutic drugs and near-infrared photosensitizers. The nano-drug loading system modified with hydrogen oxide enzyme used for increasing the oxygen content around the tumor tissue and overcoming the limitation of photodynamic treatment effect of the tumor tissue and surrounding hypoxia of the same; on the other hand, the problems about limitations of a single treatment mode and poor anti-tumor effect are solved; the combination of chemotherapy and photodynamic therapy is adopted, mutual promotion under various treatment modes can eliminate tumor cells more efficiently and deeply, and the anti-tumor effect is improved.

The invention relates to a preparation method of an Au/CuSe tangential heterogeneous nano material, which comprises the following steps of S1, preparing a nanogold colloidal solution under a hexadecyltrimethyl ammonium bromide system, wherein the particle size of gold nanospheres is 18-22nm; S2, preparing a sodium hydrogen selenide solution; adopting a water-phase synthesis method, taking the gold nanospheres as a growth substrate, the sodium hydrogen selenide and the copper acetate as the precursors, the ascorbic acid as a reducing agent and the hexadecyl trimethyl ammonium bromide as a surfactant, carrying out vacuum reaction in the presence of hexamethylenetetramine to grow a two-dimensional copper selenide nanodisk in the tangential direction of the gold nanospheres so as to obtain anAu/CuSe tangential heterogeneous nano-material aqueous solution; and S3, separating, cleaning and drying to obtain the Au/CuSe tangential heterogeneous nano material. The Au/CuSe tangential heterogeneous nano-material is prepared by using an all-aqueous phase method, and the obtained heterogeneous nano-material is stable in morphology and structure, obvious in double-plasmon coupling effect and excellent in photocatalytic hydrogen production performance.

The invention discloses a preparation method of a fiber bragg grating glucose sensor based on gold nanosphere particle modification. The fiber bragg grating glucose biosensor comprises a large-angle inclined fiber bragg grating, a gold nanosphere particle layer, dopamine molecules and concanavalin molecules, wherein the wide-angle fiber bragg grating is subjected to silanization treatment and thenis covalently combined with gold nanosphere particles, and then the concanavalin molecules are connected with the gold nanosphere particles through the dopamine molecular layer, so that the biological functionalization process of the wide-angle tilted fiber bragg grating is realized. When glucose molecules and the concanavalin molecules are specifically combined, the spectrum of the fiber bragg grating is changed, and the resonance wavelength in the spectrum is remarkably changed along with the increase of the concentration of a glucose solution, so that the high-sensitivity specific detection of glucose is realized. The glucose biosensor has the advantages of low temperature cross sensitivity, ultrahigh sensitivity, simplicity in operation, no marking and the like, and has an important application prospect in the aspect of clinical medical detection.

The invention discloses a detection probe, a kit and a direct detection method for telomerase activity. The detection probe comprises gold nanoparticles and a probe sequence connected with the gold nanoparticles, wherein the probe sequence comprises a connecting section and a primer section for telomerase recognition; the sequence length of the connecting segment is 5-8 nt; the length of the primer section is 15 to 20 nt. The probe sequence marked on the surfaces of the gold nanoparticles is extended by telomerase; after telomeres extend, the gold nanospheres with the surfaces covered with DNA products of different lengths are recognized through the solid nanopores, and real-time high-sensitivity monitoring of the solid nanopores on the telomerase activity is achieved. According to the invention, telomere DNA chains are fixed on the surfaces of the gold nanospheres, enzyme capture is facilitated, and the activity reaction of telomerase is improved; meanwhile, the DNA-gold nanosphere composite structure plays a signal amplification function in nanopore detection, and the telomerase activity can be directly monitored in real time.