14results about How to "Improve intake capacity" patented technology

The invention relates to a novel hexahydropyrroloindole alkaloid extracted from fructus lycii, which can effectively solve the problems of extracting and preparing the novel hexahydropyrroloindole alkaloid A from the fructus lycii and realizing the application of the novel hexahydropyrroloindole alkaloid A in preparation of medicines for preventing and treating type 2 diabetes mellitus. The novel hexahydropyrroloindole alkaloid A is prepared by the following steps: sequentially eluting with water, ethanol and acetone, carrying out reduced pressure concentration on an ethanol part, extracting, stirring a sample with silica gel, carrying out gradient elution, merging the same fractions, eluting a target component, stirring the sample with silica gel, carrying out gradient elution, collecting fractions within retention time, concentrating and drying, thereby obtaining the novel hexahydropyrroloindole alkaloid A in the preparation of the medicine for preventing and treating the type 2 diabetes mellitus and the application of the novel hexahydropyrroloindole alkaloid A in the preparation of the medicine for preventing and treating the type 2 diabetes mellitus. Glucose consumption in insulin-resistant HepG2 cells can be remarkably promoted, glucose uptake capacity is improved, glycogen synthesis is promoted, activity of glycolysis key enzymes hexokinase and pyruvate kinase is enhanced, development and application prospects are wide, and economic and social benefits are remarkable.

The invention discloses a preparation process of rebamipide tablets, and relates to the technical field of pharmaceutical preparations. When preparing rebamipide tablets, the present invention first reacts BOC-lysine tert-butyl ester hydrochloride with isocyanoethyl methacrylate to obtain lysine monomer; lysine monomer is reacted with terminal 2-bromoisobutyrate polylactic acid to obtain an amino acid amphiphilic block copolymer; amino acid amphiphilic block copolymer is used to load rebamipide to obtain a loaded rebamipide amphiphilic copolymer; dopa is reacted with chitosan to obtain dopa-modified chitosan; dopa-modified chitosan and bismuth salt are film-coated on the surface of the loaded rebamipide amphiphilic copolymer to obtain dopa-modified chitosan-coated rebamipide, and then dopa-modified chitosan-coated rebamipide is made into tablets to obtain rebamipide tablets. The rebamipide tablets prepared by the present invention have the advantages of mucosal adhesion, long in vivo retention time, and high cellular uptake.

The invention discloses a protein variant combination for improving the immune function of iNKT cells and application, and relates to the technical field of tumor immunity. Comprising a protein variant combination consisting of a protein variant ANT A3, a protein variant GLUT3G1, a protein variant LDHB B2, a protein variant PC P4 and a protein variant PCK1K2; according to the invention, gene sequences of the five protein variants are simultaneously embedded into an iNKT cell genome in a fixed-point manner for the first time to form a metabolic reconstitution MetaR-iNKT cell, and it is found that when the MetaR-iNKT cell and an ATP mitochondrial transporter protein inhibitor PENAO are combined for use, lung cancer solid tumors can be synergistically and better killed.

The invention discloses an OVA (at) MMSNs (at) BM-Mannose bionic nano vaccine and a preparation method thereof, and belongs to the technical field of nano vaccines. The OVA (at) MMSNs (at) BM-Mann rose bionic nano vaccine is a nano material coated by a bacterial plasma membrane. The OVA (at) MMSNs (at) BM-Mannose bionic nano vaccine prepared by the preparation method disclosed by the invention is strong in cellular uptake ability and good in storage stability; the hemolytic activity is low, and the hemolysis rate of 6% or below can still be kept even when the concentration is 100 micrograms per milliliter; the in-vitro cytotoxicity is small, and the safety is high; obvious toxicity is not generated on mice in vivo; the hydration particle size is 220nm; dC2.4 cells have the phenomena of continuous uptake and concentration dependence on the nano vaccine, are basically saturated within 2 hours, and have the potential of being applied to the field of magnetic resonance imaging; the activation efficiency on DC2.4 is high; the targeting delivery capability is realized; according to the prepared FITC-OVA (at) MMS Ns (at) BM-Mannose bionic nano vaccine, the visual delivery of the nano vaccine can be realized, and the observation and research are convenient; the preparation method of the bionic nano vaccine is simple and easy to operate, and the used raw materials are safe, non-toxic or low in toxicity and safe to the environment and operators.

This invention discloses an enzyme- and pH-responsive nucleic acid loading and delivery material and its preparation method. The material can be used for efficient loading and delivery of nucleic acid molecules. The preparation method uses amphiphilic zwitterionic monomers to improve the reverse emulsion polymerization system, enhancing polymerization at the two-phase interface and avoiding the extrusion effect of polymerization in the aqueous core on nucleic acid molecules, thereby improving the loading efficiency of nucleic acid molecules. Further preparation of cross-linking agents MP-CL and CB-CL, which can cleave in response to matrix metalloproteinase II or cathepsin B, endows the nanogel with the ability to respond to charge reversal in the tumor matrix and to release nucleic acid molecules from tumor cells. The acid-sensitive blocks on the amphiphilic monomers give the nanogel lysosomal escape capability. The delivery material can efficiently deliver nucleic acid molecules into cells and exhibits excellent stability and biosafety.

The invention discloses a compound polysaccharide preparation for treating diabetes and application thereof, and belongs to the technical field of biomedicine. The compound polysaccharide preparation is composed of compound polysaccharide A and compound polysaccharide B according to the mass ratio of 1: 1. The compound polysaccharide A is prepared from abelmoschus esculentus and chlorella through alkaline ultrasonic extraction and 10-100kDa ultrafiltration classification; the compound polysaccharide B is prepared by performing enzymolysis and hot water extraction on grifola frondosa and sarcodon aspratus. Experiments prove that the preparation can significantly improve insulin secretion and glucose uptake ability of islet cells, effectively reduce fasting blood-glucose of type 2 diabetes model mice and synchronously improve triglyceride and total cholesterol levels through strong synergistic interaction among the components. The preparation disclosed by the invention is natural in source, safe and efficient, so that a new choice is provided for medicine development of type 2 diabetes mellitus.

The present invention belongs to the field of new biomedical technology applications, and specifically relates to a targeted polypeptide and a radioactive tracer for brain glioma. The amino acid sequence of the polypeptide is: KINWKFRLRY, Lysine-Isoleucine-Asparagine-Tryptophan-Lysine-Phenylalanine-Arginine-Leucine-Arginine-Tyr, Lys-Ile-Asn-Trp-Lys-Phe-Arg-Leu-Arg-Tyr. The radioactive tracer is represented by: 68 Ga‑DOTA‑KINW, where, 68 Ga is a radionuclide, DOTA is a chelating agent, and KINW is a polypeptide targeting the NPY1R receptor protein. The present invention uses mutation scanning and molecular docking technology to screen and modify a targeting polypeptide that specifically binds to NPY1R. Compared with the sequences reported previously, KINW has more binding sites to NPY1R and less cytotoxicity. KINW can cross the blood-brain barrier and 68 Ga chelation can image in situ brain glioma; the radioactive tracer prepared by the invention is used as an imaging drug for brain glioma, and has the characteristics of long imaging time, easy preparation and the like.

This invention discloses a metabolically enhanced anti-tumor CAR-M cell co-expressing SLC protein, comprising the nucleotide sequence encoding the HER2 antigen-binding domain scFv and the nucleotide sequence encoding the SLC protein. The invention also discloses a method for constructing the aforementioned anti-tumor CAR-M cell and its application in the preparation of anti-tumor drugs. The anti-tumor CAR-M cell of this invention specifically recognizes HER2, thereby effectively reducing off-target toxicity, improving treatment safety and tumor specificity, and exhibiting significantly enhanced glutamine uptake capacity. It possesses a good metabolic reprogramming effect at the molecular level, resulting in stronger anti-tumor activity due to enhanced metabolism.

This invention belongs to the field of biomedical materials technology, and relates to a signal probe that simultaneously possesses CT and MRI imaging capabilities, its preparation method, and its application. The preparation method includes the following steps: dissolving bismuth citrate and iron salt in water, adjusting the pH of the solution to <7, adding polyvinylpyrrolidone, then adding gallic acid, adjusting the pH of the mixed solution to >7, and after the reaction is complete, filtering through a membrane to remove impurities, dialysis, and drying to obtain bismuth-iron nanoparticles; mixing citric acid with the bismuth-iron nanoparticle solution, stirring and reacting to obtain citric acid-treated bismuth-iron nanoparticles; further activating to obtain an activated bismuth-iron nanoparticle solution; adding RGDfk and amino-terminated polyethylene glycol to the activated bismuth-iron nanoparticle solution, stirring and reacting in the dark, dialysis, and drying to obtain a signal probe that simultaneously possesses CT and MRI imaging capabilities. The signal probe prepared by this invention has a small particle size and simultaneously possesses CT and MRI imaging functions.

The invention relates to an efficient polypeptide photosensitizer and a preparation method and application thereof, and relates to the technical field of medicine, the structure of the efficient polypeptide photosensitizer is A-B-A, A is a polypeptide sequence with positive charges, and B is a photodynamic therapy photosensitizer. According to the high-efficiency polypeptide photosensitizer prepared by the invention, the formed nanoparticles enhance the permeation of tumor tissues, selectively transmit the photosensitizer to a tumor site, enhance the targeting of tumors, realize the retention of the photosensitizer at the tumor site, and relieve the aggregation of photosensitizer monomers, so that a better photodynamic therapy effect is achieved. The method is expected to provide a better treatment method for photodynamic tumor treatment, and a new thought is provided for realizing intelligent delivery and improving the treatment effect.

The invention discloses a process for preparing a liquid fertilizer by using proline fermentation liquor, and relates to the technical field of fertilizer preparation, and the process has the advantages that an acrylic monomer is combined with an ammonium persulfate initiator and an N, N '-methylene bisacrylamide cross-linking agent to prepare a high-molecular polymer network, and in the process, the proline fermentation liquor is added into the high-molecular polymer network to prepare the liquid fertilizer. The carboxyl and amino of the acrylic monomer provide active sites for subsequent reaction, then a copper ion complexing group is introduced, copper ions are subjected to complexing reaction with the carboxyl and amino in the polymer to form a stable coordination bond which is combined with electrostatic interaction, proline molecules can be efficiently grabbed from proline fermentation waste liquid, and the proline can be efficiently separated from the proline fermentation waste liquid. Specific functional groups such as acylamino are introduced, so that the specific binding with the proline is enhanced, the binding of the specific material to the proline is firmer and more accurate, a solid foundation is laid for the subsequent preparation of high-quality fertilizers, the recycling rate of resources is increased, and a high-purity key raw material is provided for the production of liquid fertilizers.

The invention discloses dendrimer functionalized fluorescent carbon nanoparticles, a preparation method and application thereof. First, citric acid and triaminobenzene are hydrothermally synthesized into fluorescent carbon nanoparticles FCNs, and then an EDC coupling method is used to form an amide bond between the carboxyl group of the fluorescent carbon nanoparticles and the amino group of PAMAM, and PAMAM is coupled to the surface of the fluorescent carbon nanoparticles through covalent functionalization to obtain dendrimer modified carbon nanoparticles PAMAM@FCNs. It has the two characteristics of excitation light independence and long wavelength emission, can effectively deliver siRNA across biological membranes into cells, and has potential in the research of new imaging tracing gene delivery nanocarriers. Compared with the prior art, the preparation method of the invention is simple, easy to operate, low in cost, and suitable for promotion.

The invention discloses a silverweed cinquefoil root active composition for improving hypoxia insulin resistance and application thereof, and belongs to the technical field of pharmaceutical compositions.The silverweed cinquefoil root active composition comprises 25-35 parts of a silverweed cinquefoil root n-butyl alcohol extract, 10-20 parts of a silverweed cinquefoil root ethanol extract, 15-25 parts of a silverweed cinquefoil root water-soluble extract and 5-10 parts of a composite adjusting extract. The combination of the potentilla anserine n-butyl alcohol extract, the potentilla anserine ethanol extract and the potentilla anserine water-soluble extract can improve insulin resistance by regulating insulin signal transduction and glucose transport, enhance glucose uptake ability, regulate oxidative stress reaction under hypoxia stress and reduce occurrence of glucose metabolism disorder; the addition of the compound regulating extract can compensate the regulating effect of various polar extracts of the potentilla anserine on a cell insulin pathway, obviously enhance the overall oxidation resistance and reduce the influence of oxidative stress on an organism, thereby improving or inhibiting the injury of insulin resistance caused by a low-oxygen environment on the organism.

The invention belongs to the field of biomedical polymer materials, and relates to a responsive hydrophilic polymer coated nano-particle drug loading system as well as a preparation method and application thereof. The nano drug loading system comprises a nano core and a hydrophilic polymer shell covering the surface of the nano core, the nano core is formed by a nano drug, the nano drug is provided with an active functional group, the hydrophilic polymer shell is formed by a hydrophilic polymer, and the hydrophilic polymer shell is provided with an active functional group. The hydrophilic polymer comprises a structural unit with carboxyl, a structural unit with responsive functional groups and a hydrophilic structural unit, the responsive functional group on the hydrophilic polymer and the active functional group on the nano-drug can form a chemical bond through a chemical reaction, so that the hydrophilic polymer is coated on the surface of the nano-drug to form a nano-drug loading system; the nano drug-loading system can be subjected to chemical bond breakage under an acidic condition to release drugs and / or can utilize tumor microenvironment to respond to chemical bonds to release drugs.