4results about How to "Improve intake capacity" patented technology

This invention discloses an enzyme- and pH-responsive nucleic acid loading and delivery material and its preparation method. The material can be used for efficient loading and delivery of nucleic acid molecules. The preparation method uses amphiphilic zwitterionic monomers to improve the reverse emulsion polymerization system, enhancing polymerization at the two-phase interface and avoiding the extrusion effect of polymerization in the aqueous core on nucleic acid molecules, thereby improving the loading efficiency of nucleic acid molecules. Further preparation of cross-linking agents MP-CL and CB-CL, which can cleave in response to matrix metalloproteinase II or cathepsin B, endows the nanogel with the ability to respond to charge reversal in the tumor matrix and to release nucleic acid molecules from tumor cells. The acid-sensitive blocks on the amphiphilic monomers give the nanogel lysosomal escape capability. The delivery material can efficiently deliver nucleic acid molecules into cells and exhibits excellent stability and biosafety.

The invention discloses a compound polysaccharide preparation for treating diabetes and application thereof, and belongs to the technical field of biomedicine. The compound polysaccharide preparation is composed of compound polysaccharide A and compound polysaccharide B according to the mass ratio of 1: 1. The compound polysaccharide A is prepared from abelmoschus esculentus and chlorella through alkaline ultrasonic extraction and 10-100kDa ultrafiltration classification; the compound polysaccharide B is prepared by performing enzymolysis and hot water extraction on grifola frondosa and sarcodon aspratus. Experiments prove that the preparation can significantly improve insulin secretion and glucose uptake ability of islet cells, effectively reduce fasting blood-glucose of type 2 diabetes model mice and synchronously improve triglyceride and total cholesterol levels through strong synergistic interaction among the components. The preparation disclosed by the invention is natural in source, safe and efficient, so that a new choice is provided for medicine development of type 2 diabetes mellitus.

This invention discloses a metabolically enhanced anti-tumor CAR-M cell co-expressing SLC protein, comprising the nucleotide sequence encoding the HER2 antigen-binding domain scFv and the nucleotide sequence encoding the SLC protein. The invention also discloses a method for constructing the aforementioned anti-tumor CAR-M cell and its application in the preparation of anti-tumor drugs. The anti-tumor CAR-M cell of this invention specifically recognizes HER2, thereby effectively reducing off-target toxicity, improving treatment safety and tumor specificity, and exhibiting significantly enhanced glutamine uptake capacity. It possesses a good metabolic reprogramming effect at the molecular level, resulting in stronger anti-tumor activity due to enhanced metabolism.

This invention belongs to the field of biomedical materials technology, and relates to a signal probe that simultaneously possesses CT and MRI imaging capabilities, its preparation method, and its application. The preparation method includes the following steps: dissolving bismuth citrate and iron salt in water, adjusting the pH of the solution to <7, adding polyvinylpyrrolidone, then adding gallic acid, adjusting the pH of the mixed solution to >7, and after the reaction is complete, filtering through a membrane to remove impurities, dialysis, and drying to obtain bismuth-iron nanoparticles; mixing citric acid with the bismuth-iron nanoparticle solution, stirring and reacting to obtain citric acid-treated bismuth-iron nanoparticles; further activating to obtain an activated bismuth-iron nanoparticle solution; adding RGDfk and amino-terminated polyethylene glycol to the activated bismuth-iron nanoparticle solution, stirring and reacting in the dark, dialysis, and drying to obtain a signal probe that simultaneously possesses CT and MRI imaging capabilities. The signal probe prepared by this invention has a small particle size and simultaneously possesses CT and MRI imaging functions.