32 results about "Plga microspheres" patented technology

The invention relates to a concrete anti-cracking self-repairing agent in the technical field of building materials, and aims to solve the problem that concrete cracks are difficult to self-repair. According to the technical scheme, the self-repairing agent is characterized by comprising a microbial repairing unit formed by ZSM-5 type zeolite molecular sieve loaded bacillus pasteurii spores, mesoporous silica particles loaded with L-calcium aspartate and Fe3O4 (at) SiO2 core-shell nanoparticles which are fixed through an in-situ polymerization coating layer to form a magnetic thermal response capsule layer coating the surface of the microbial repairing unit, pLGA microspheres containing yeast extract are positioned on an interface of zeolite and hydrogel, and a trehalose-anhydrous calcium chloride eutectic compound is used as a directional mineralization accelerant. The concrete anti-cracking self-repairing agent provided by the invention is mainly used for intelligent identification and self-repairing of concrete cracks, and efficient repairing is realized through a microbial mineralization, calcium ion slow release and magnetic thermal response synergistic mechanism.

The invention provides a pregabalin slow-release type dissolving microneedle patch based on PLGA microspheres as well as a preparation method and application thereof. The method comprises the following steps: dissolving pregabalin and hyaluronic acid in deionized water to form an inner water phase (W1); dissolving PLGA in dichloromethane to form an organic phase (O); performing ultrasonic emulsification on the internal water phase and the organic phase to form W / O primary emulsion; rapidly adding the primary emulsion into an external water phase (W2) containing PVA and NaCl to form a W1 / O / W2 type multiple emulsion, and then carrying out solvent evaporation curing, centrifugal washing and freeze drying to obtain PLGA (at) PG microspheres; and uniformly dispersing the PLGA (at) PG microspheres in a hyaluronic acid solution, adding into a microneedle mold, vacuumizing, centrifuging, drying, and demolding the microneedle to obtain the dissolution type microneedle patch. By optimizing microsphere preparation, drug sustained release and microneedle patch preparation technologies are realized, and an efficient, safe and continuous local delivery platform is provided.

The invention discloses composite hydrogel bio-ink with a function of space-time delivery of double growth factors as well as a preparation method and application of the composite hydrogel bio-ink, and belongs to the technical field of biomedical materials and tissue engineering. According to the preparation method, imidazolyl-modified methacrylated gelatin and double-bonded PF127 are compounded, and meanwhile, free vascular endothelial growth factors and connective tissue growth factor-loaded PLGA microspheres are entrapped in a system, so that the bio-ink with excellent printability, high adhesion, swelling resistance and space-time delivery function is formed; the bio-ink physically has the advantages of high printing fidelity, low swelling property, high mechanical strength and strong tissue adhesion of a synthetic material; in biology, the system can be used as an intelligent carrier to realize programmed space-time delivery of various growth factors, so that complex cell biological behaviors are accurately arranged, and real regeneration of functional tissues is guided.

The embodiment of the invention provides nano-enzyme hydrogel for promoting scar-free wound repair through inflammation regulation and c-Jun gene silencing and a preparation method of the nano-enzyme hydrogel. The nano-enzyme hydrogel is prepared from the following components in parts by mass: synthesized epsilon-polylysine modified CeO2 nano-enzyme particles and aldehyde hyaluronic acid, an instant Schiff base reaction between epsilon PL-CeO2 surface amino groups and HA-CHO molecule aldehyde groups is utilized, in-situ mixing is performed to form a hydrogel network, in addition, bFGF and siRNA are loaded into PLGA microspheres, and the hydrogel network is formed through in-situ mixing. And further embedding into the hydrogel to obtain the nano-enzyme hydrogel. The obtained nano-enzyme hydrogel has the functions of inflammation regulation, cell proliferation promotion, vascular regeneration promotion, c-Jun gene silencing and the like, and scar-free healing of skin wounds is remarkably promoted.

The invention discloses CaHA / PLGA (poly (lactic-co-glycolic acid)) composite microspheres, a preparation method and application of the CaHA / PLGA composite microspheres in a soft tissue filler. The preparation method of the composite microspheres comprises the following steps: dissolving PLGA in dichloromethane to obtain a first solution, dissolving polyvinyl alcohol in water to obtain a second solution, mixing the two solutions, continuously stirring until dichloromethane is completely volatilized, and freeze-drying to obtain PLGA microspheres; the preparation method comprises the following steps: adding calcium salt and phosphate into water, dissolving, regulating the pH until the solution is clear, adding urea and a template agent, dissolving, and carrying out hydrothermal reaction to obtain CaHA microspheres; carrying out alkali treatment on the PLGA microspheres to obtain activated PLGA microspheres, dispersing CaHA microspheres in a dispersing agent, adding the activated PLGA microspheres into the dispersion liquid, oscillating, and freeze-drying to obtain the product. The soft tissue filling agent is prepared from CaHA / PLGA (poly (lactic-co-glycolic acid)) composite microspheres and gel. The mechanism of the CaHA / PLGA composite microspheres for stimulating collagen regeneration is milder, the inflammation risk is lower, and the defects of a traditional soft tissue filling agent can be overcome.

The invention relates to the technical field of biomedical materials, in particular to a biomimetic ECM activating factor inflammation response type sustained release microsphere system, which comprises: a) core-shell structure PLGA microspheres, the core of which is loaded with USAG-1 recombinant protein (the drug loading capacity is greater than or equal to 1.5 mu g / mg), and the shell of which is composed of an MMP-9 sensitive peptide crosslinking layer; b) co-modifying nano-hydroxyapatite (nHA) and RGD peptide on the surface of the microsphere, wherein the molar ratio of nHA to RGD is 1: 3; c) uniformly dispersing the BMP-2 in the PLGA matrix to realize zero-order slow-release kinetics; the precise time sequence controlled release and microenvironment responsiveness are as follows: 1) inflammatory stage targeted release: when the local MMP-9 concentration is greater than 15nM, the shell GPLGVRGD peptide crosslinking layer is rapidly degraded (release is greater than or equal to 65% USAG-1 within 24h), premature activation of BMP-2 is inhibited, and the ectopic ossification occurrence rate is reduced from 24.6% to 3.8% (p is less than 0.001); (2) continuously promoting regeneration in a repair period: slowly releasing BMP-2 (53.2 + / -2.1% accumulatively released in 56 days) by a PLGA core through zero-order dynamics, maintaining a stable osteogenesis signal, and increasing the volume ratio of new bones to 58.7% (22.3% of a control group);

The invention provides a kaempferol nano drug delivery system which comprises PLGA (poly (lactic-co-glycolic acid)), kaempferol and mannose, a drug is entrapped by the PLGA to form PLGA microspheres, and the surfaces of the PLGA microspheres are coupled with mannose nano-particles with a targeting function through an amide reaction; the invention protects the application of the kaempferol nano drug delivery system in rheumatoid arthritis. The kaempferol nano drug delivery system is a nano drug delivery system with a mannose receptor targeting characteristic. The invention relates to the field of targeted drugs, in particular to a kaempferol nano drug loading system which is a drug loading system for treating rheumatoid arthritis. The drug loading system for treating rheumatoid arthritis provided by the invention not only has higher encapsulation efficiency and drug loading capacity, but also is lower in preparation cost, also has excellent biocompatibility and biological safety, and has a more excellent anti-rheumatoid arthritis effect.

The invention belongs to the technical field of biomedicine, and particularly discloses an endowed mesenchymal stem cell, an endowed mesenchymal stem cell composition and application of the endowed mesenchymal stem cell composition to preparation of a medicine for treating organ insufficiency, and the endowed mesenchymal stem cell is obtained by treating human mesenchymal stem cells with ingenol HEP14. The energizing mesenchymal stem cell composition comprises energizing mesenchymal stem cells and HEP14 / PLGA microspheres which are prepared by adopting a polylactic acid-glycolic acid copolymer (PLGA) as a carrier and have high HEP14 loading capacity and long-acting HEP14 slow release. The energized mesenchymal stem cell has the effects of enhancing the survival activity and dryness of the mesenchymal stem cell, resisting tissue fibrosis, promoting tissue angiogenesis, and promoting tissue regeneration and functional recovery; the endowed mesenchymal stem cells and the HEP14 / PLGA microspheres are used in a combined manner, and the retention and transdifferentiation effects of the endowed mesenchymal stem cells in tissues are enhanced, so that the functions of the endowed mesenchymal stem cells and the application of the endowed mesenchymal stem cells in the field of regenerative medicine are further enhanced.

The invention relates to a spinal cord injury repair method based on four-dimensional multifunctional hydrogel, which comprises the following steps: firstly, encapsulating NT-3 in PLGA (poly (lactic-co-glycolic acid)) microspheres through a microsphere technology, and integrating HCT (Hydroxycycline Computed Tomography) into SilMA hydrogel, so as to construct four-dimensional multifunctional hydrogel 4DMSH with time specificity; the hydrogel shows a remarkable anti-inflammatory effect in an in-vivo experiment, can effectively inhibit the inflammatory reaction at the early stage of spinal cord injury, promotes eNSCs to differentiate into neurons by improving a microenvironment and inhibits formation of glial scars, so that the nerve repair efficiency is improved; the four-dimensional multifunctional hydrogel has time specificity and can create an anti-inflammatory and neuroactive microenvironment, so that effective spinal cord injury repair and nerve regeneration are realized; the hydrogel can be widely applied to SCI treatment, and a brand new strategy is provided for repairing nervous system injury.

This invention discloses a microsphere-oil gel composite sustained-release injection and its preparation method. The method includes the following steps: dissolving PVA in pure water to obtain an aqueous phase; dissolving PLGA in an organic solvent to form an organic phase; adding vitamin B12 in solid form to the organic phase to form a solid-oil phase suspension system; adding the solid-oil phase suspension system to the aqueous phase for emulsification to form a solid-oil-aqueous emulsion; adding the emulsion to excess pure water to form drug-loaded PLGA microspheres and freeze-drying; using peanut oil as the oil phase, heating to 60-65°C, adding a gelling agent (a mixture of glyceryl monostearate and beeswax) to the oil phase to form a clear and homogeneous oil phase system; cooling the oil phase system to 30-40°C, adding drug-loaded PLGA microspheres, and uniformly dispersing them under stirring conditions, then continuing to cool naturally to room temperature to obtain the microsphere-oil gel composite sustained-release injection.

The invention relates to the technical field of keratoprosthesis preparation, in particular to a biomimetic structure keratoprosthesis carrying a PRP microsphere slow release system and a preparation method of the biomimetic structure keratoprosthesis. PRP is encapsulated through PLGA microspheres, and stepped release of growth factors is achieved through degradation controllability of PLGA; the PRP-PLGA microspheres still keep a stable slow-release effect within 14 days in vitro, the problem that growth factors are rapidly inactivated due to direct application of traditional PRP is effectively solved, the treatment window period is remarkably prolonged, and continuous biological activity stimulation needed by cornea repair is met; by utilizing the degradation controllability of the PLGA, the stepped release of the growth factor is realized, so that the stable release effect of the PRP can be maintained for a long time after the artificial cornea is transplanted, the treatment window period after the artificial cornea is transplanted is prolonged, and the continuous biological activity stimulation required by cornea repair is met.

The invention discloses a preparation method of multifunctional microspheres loaded with curcumin-hydroxyapatite-PLGA (poly (lactic-co-glycolic acid)), which comprises the following steps: step 1, weighing raw material medicines and PLGA in a beaker, adding an organic solvent into the raw material medicines and the PLGA, and carrying out ultrasonic treatment until the raw material medicines and the PLGA are completely dissolved to form a transparent oil-phase solution; wherein the raw material medicines comprise curcumin and hydroxyapatite; adding ultrapure water into the weighed polyvinyl alcohol emulsifier, and stirring until the emulsifier is completely dissolved to form a water phase solution; step 2, dropwise adding the organic phase into the aqueous phase solution, and carrying out continuous stirring and ultrasonic treatment, so that the organic phase is uniformly dispersed in the aqueous phase to form primary emulsion; 3, stirring the obtained primary emulsion, and removing the organic solvent in the primary emulsion; and centrifuging, washing and precipitating the primary emulsion without the organic solvent, removing the free emulsifier and the unencapsulated medicine, and carrying out vacuum drying to obtain the Cur-HA / PLGA microspheres. The curcumin and the nano-hydroxyapatite can be jointly loaded on the PLGA microspheres, so that the antibacterial, anti-inflammatory and bone regeneration promoting synergistic effect is realized.

The application discloses a medical dressing for wound repair and a preparation method thereof. The dressing comprises a gel matrix and modified growth factor-encapsulated PLGA microspheres. The modified growth factor-encapsulated PLGA microspheres are growth factor-encapsulated PLGA microspheres modified with alkali lignin on the surface. The modified growth factor-encapsulated PLGA microspheres can effectively prevent ultraviolet damage of growth factors and improve bioavailability. Meanwhile, the modified growth factor-encapsulated PLGA microspheres have a suitable degradation period, can release growth factors to promote cell proliferation and wound healing in the proliferation period of a wound, and are stably dispersed in an aqueous phase, so that the dispersion uniformity of the modified growth factor-encapsulated PLGA microspheres in the dressing is improved, and batch stability of the dressing is improved.

The invention provides composite recombinant collagen peptide hydrogel as well as preparation and application thereof, and relates to the field of biomedical materials. The hydrogel comprises the following components: recombinant III-type collagen peptide, recombinant I-type collagen peptide, oxidized chondroitin sulfate, cystamine modified polyethylene glycol and antibacterial peptide loaded PLGA (poly (lactic-co-glycolic acid)) microspheres, wherein the oxidized chondroitin sulfate and amino groups of the collagen peptide are cross-linked through Schiff base bonds to form a first network; sulfydryl of the cystamine modified polyethylene glycol and carboxyl of the collagen peptide are cross-linked through disulfide bonds to form a second network. A double-network structure is constructed through a Schiff base bond and a disulfide bond, the mechanical strength and fatigue resistance of the hydrogel are remarkably improved, the hydrogel has excellent biocompatibility, a mild light-operated crosslinking process is adopted, active ingredients are prevented from being damaged by a high temperature / chemical initiator, and the hydrogel is particularly suitable for diabetic foot ulcer wound repair.

The invention discloses a medical dressing for wound repair and a preparation method thereof. The dressing comprises a gel matrix and modified PLGA (poly (lactic-co-glycolic acid)) microspheres for encapsulating growth factors, the modified PLGA microspheres for encapsulating the growth factors are PLGA microspheres for encapsulating the growth factors, and the surfaces of the PLGA microspheres are modified with alkali lignin. The modified PLGA microspheres encapsulating the growth factors can effectively prevent ultraviolet damage of the growth factors, and the bioavailability is improved; meanwhile, an appropriate degradation period is achieved, and growth factors can be slowly released in the proliferation period of the wound to promote cell proliferation and wound healing; furthermore, the PLGA microspheres with the alkali lignin surface modified and encapsulated with the growth factors are stably dispersed in a water phase, so that the dispersion uniformity of the PLGA microspheres in the dressing is improved, and the batch stability of the dressing is improved.

The invention belongs to the technical field of biological medicine, and particularly relates to a lamivudine prodrug long-acting PLGA microsphere preparation and a preparation method thereof. The lamivudine prodrug long-acting PLGA microsphere comprises a lamivudine fatty acid ester prodrug and a PLGA carrier material, the structural general formula of the lamivudine fatty acid ester prodrug is [lamivudine mother nucleus]-O-CO-(CH2) n-CH3, n is an integer of 4-18, and the lamivudine fatty acid ester prodrug is formed by connecting lamivudine and fatty acid through an amido bond; the microspheres are prepared by adopting a single emulsion solvent evaporation method, and the prepared microspheres have the advantages of high drug loading capacity (5%-11%), high encapsulation efficiency (36%-98%) and low burst release effect (24 h lt) by optimizing a prodrug structure, PLGA material characteristics and process parameters; 10%) and the like, and stable drug release of more than 4 weeks can be realized.

The invention discloses an inner ear local release hydrogel and a preparation method thereof, and belongs to the field of biomedical materials and drug delivery. PLGA microspheres are used as model particles, after the PLGA microspheres are wrapped by polydopamine, a diol structure is introduced to the surface, and the PLGA microspheres and a phenylboronic acid grafted hyaluronic acid main chain form a stable borate bond under a neutral condition, so that a three-dimensional injectable hydrogel network is constructed under the condition that no extra small molecule cross-linking agent is needed. The drug-release hydrogel provided by the invention has good biocompatibility, adhesion and slow release ability, can prolong local drug residence time, improves the drug concentration of inner ears, and significantly improves the treatment effect.

The invention discloses a ziconotide-loaded PLGA (poly (lactic-co-glycolic acid)) sustained-release microsphere as well as a preparation method and application thereof. The PLGA microspheres are mainly prepared from a polylactic acid-glycolic acid copolymer (PLGA), ziconotide and trehalose; the PLGA microspheres are prepared from the following components in percentage by mass: 85.2 to 95.6 percent of PLGA, 4.4 to 8.2 percent of ziconotide and 0 to 7.4 percent of trehalose, ziconotide medicine is uniformly dispersed in PLGA, microspheres are prepared by adopting a double-emulsification method, and stable medicine carrying microspheres are formed. The PLGA microspheres prepared by the invention can realize long-time slow release of ziconotide, significantly prolong the drug effect maintenance time, and show relatively low toxic and side effects in vivo; by means of controllable degradation of the PLGA material, ziconotide can be continuously released in vivo, the bioavailability of the medicine is improved, the compliance burden of a patient is reduced, the defects of an existing ziconotide administration mode are overcome, and an efficient, safe and sustainable slow-release treatment scheme is provided.

The application belongs to the field of biological pharmacy, and relates to a temperature-sensitive hydrogel, in particular to a temperature-sensitive hydrogel SV@HP based on hyaluronic acid and a preparation method and application thereof; the temperature-sensitive hydrogel SV@HP is used for loading CS-siRNA nanoparticles and VEGF@PLGA microspheres, and is used for realizing microenvironment-responsive drug delivery to treat intrauterine adhesion. The application uses CS-siRNA nanoparticles of chlorogenic acid and TGFbeta1 siRNA self-assembled through a disulfide bond, is used for gene and chemotherapy to treat intrauterine adhesion, and proves to have a good effect of improving intrauterine adhesion through cell and animal experiments. Through evaluation by real-time fluorescent quantification, Western blotting, a cell scratch test, laser confocal microscope observation, inverted fluorescence microscope observation and in vivo experiments, it is found that the SV@HP gel has the effects of anti-inflammation, improvement of endometrial fibrosis, promotion of endometrial angiogenesis and repair of IUA rats, inhibition of intrauterine adhesion and improvement of intrauterine environment.

The present invention discloses a preparation method and application of an immune nanocomposite hydrogel with anti-tumor effect, wherein dopamine-modified hyaluronic acid is added to water to form a dopamine-modified hyaluronic acid aqueous solution, δ-ALA@PLGA microspheres are then added, dopamine is added, sodium periodate is added, dopamine polymerization is induced under stirring, and then the mixture is allowed to stand to form a polydopamine hydrogel; sodium alginate and aCD47@CaCO3 nanoparticles are added to water to obtain a sodium alginate solution; the sodium alginate solution is added to the polydopamine hydrogel, calcium chloride solution is added, and the mixture is allowed to stand. The present invention can achieve the production of hydrogel by simple mixing, has a short reaction cycle, a high yield, no by-products, is easy to realize industrial production, and can effectively improve work efficiency. The composite hydrogel can be used as a new bioactive material for tumor treatment and can be used as a gel patch that adheres to the lesion and releases drugs for a long time.

A spinal cord injury repair method based on biphase fibroin and methacryloyl hydrogel comprises the following steps: firstly, encapsulating NT-3 and NSCs in PLGA microspheres through a microsphere technology, and integrating Ang-(1-7) into SilMA hydrogel, so as to construct the biphase SilMA multifunctional hydrogel (DPSH) with time-specific drug controlled release. The hydrogel shows a remarkable anti-inflammatory effect in an in-vivo experiment, can effectively inhibit the inflammatory reaction at the early stage of spinal cord injury, promotes NSCs to be differentiated into neurons by improving a microenvironment and inhibits formation of glial scars, so that the nerve repair efficiency is improved.

The invention belongs to the technical field of biomedical materials, and provides an intelligent hydrogel dressing with a three-layer structure as well as a preparation method and application of the intelligent hydrogel dressing. The intelligent hydrogel dressing with the three-layer structure comprises an upper layer, a middle layer and a bottom layer which are sequentially stacked, the upper layer is methacrylated gelatin hydrogel containing silver nanoparticles, the middle layer is methacrylated gelatin hydrogel containing mangiferin-loaded PLGA microspheres, and the bottom layer is methacrylated gelatin hydrogel. The upper layer of the intelligent hydrogel dressing with the three-layer structure is used for broad-spectrum antibiosis in the initial stage and quickly coping with bacterial infection; the middle layer is used for continuously releasing mangiferin, exerting the effects of resisting oxidation, resisting inflammation and promoting angiogenesis and eliminating oxidative stress; the bottom layer provides mechanical support and biocompatibility; through the synergistic effect of the multi-layer structure of the upper layer, the middle layer and the bottom layer, remarkable advantages are shown in multiple aspects such as mechanical support, medicine controllable release, broad-spectrum antibiosis and inflammation-oxidative stress regulation, and layering and cooperative intervention are achieved.

The invention discloses a degradable sclera fixing ring carrying a gene editing carrier. The degradable sclera fixing ring comprises a fixing ring, a glass slide and a baffle ring, a plurality of uniformly distributed grooves are formed in the fixing ring, a glass slide is carried in the fixing ring, and the baffle ring is located above the glass slide. Compared with the prior art, the preparation method has the advantages that the PLGA microspheres are embedded in the microspheres to wrap the gene carrier, the gene carrier is dispersed in the pores of the matrix, the surface of the matrix is modified by the cationic polymer through electrostatic adsorption, negatively charged nucleic acid and a hydrogel slow-release layer are adsorbed, and the hydrogel slow-release layer is a thermo-sensitive hydrogel loading carrier, so that on-demand release is realized.

This invention discloses a multi-microsphere-coated drug carrier, its preparation method, and its application. The drug carrier uses drug-loaded attapulgite particles as a core, with the core surface coated by interconnected PLGA microspheres. The surface of the drug carrier is uneven and porous. The preparation method of the drug carrier involves: obtaining attapulgite particles through spray granulation, loading the drug into the attapulgite particles, and then coating the attapulgite particles with PLGA. The drug carrier provided by this invention exhibits good sustained-release performance and safety, and is of great significance for antitumor drug delivery.

The invention relates to the technical field of pharmaceutical preparations, and particularly provides an MCP-1 response type microneedle, a microneedle patch and a preparation method and application of the MCP-1 response type microneedle and the microneedle patch. The MCP-1 response type microneedle loaded glucan modified silver nanoparticles and the heparin modified taurine PLGA microspheres are used for targeted elimination of bacteria and reprogramming of an immune microenvironment. The invention develops a double-layer microneedle system responding to a bacterial microenvironment, and by integrating antibiosis, antioxidation and M2 type macrophage polarization capability into the double-layer microneedle structure, local infection control, immune regulation and tissue regeneration can be synergistically combined, so that the key obstacle of wound repair is effectively overcome, the complex wound healing obstacle can be solved, and the wound healing effect is improved. And particularly, a comprehensive solution is provided for infectious and chronic wounds such as diabetes mellitus and the like.

The invention belongs to the field of medicines, and particularly relates to a perampanel long-acting controlled-release microsphere and a preparation method thereof. The invention aims to provide a long-acting and precise controlled-release perampanel microsphere which is prepared from an internal phase solution and an external phase solution by adopting a microfluidic method, wherein the internal phase solution comprises perampanel and a derivative thereof, PLGA (poly (lactic-co-glycolic acid)) and a good solvent of the perampanel and the derivative thereof; the external phase solution comprises a surfactant, a good solvent of the surfactant, and an internal phase solvent. According to the perampanel long-acting sustained and controlled release microspheres, the PLGA microspheres prepared through microfluidics are very uniform, can stably release drugs and have highly-stable repeatability and regularity, the perampanel-PLGA sustained release microspheres prepared through the microfluidics technology are combined with a customization strategy to be very promising, related microsphere products are also beneficial to transformation, and the perampanel long-acting sustained and controlled release microspheres are suitable for industrial production. The method is an effective solution.

The invention provides temperature-controlled drug-loading hydrogel and a preparation method and application thereof, the temperature-controlled drug-loading hydrogel comprises Dexp-coated PLGA and temperature-sensitive hydrogel, and the mass volume ratio of the Dexp-coated PLGA to the temperature-sensitive hydrogel is (10-50) mg: 1 mL; the Dexp-coated PLGA is a PLGA microsphere loaded with dexamethasone sodium phosphate, and the temperature-sensitive hydrogel is prepared from poloxamer and polycarbophil. Poloxamer and polycarbophil are adopted as temperature-sensitive hydrogel, and matched with Dexp-PLGA composed of dexamethasone sodium phosphate (Dexp) entrapped by PLGA microspheres to form temperature-control drug-loading hydrogel (Dexp-PLGA-Gel), the temperature-control drug-loading hydrogel can be automatically degraded, is not left in vivo, reduces toxic and side effects, is in a liquid state at the temperature of 4 DEG C or normal temperature, is in a gel state at the body temperature of 36 DEG C, and is free of toxic and side effects. The medicine can stay in the middle ear for a long time to play a role, the injection frequency is reduced, and the pain of a patient is relieved.

The invention discloses an induced construction method of a machin hypertension model, which comprises the following steps: mixing a PDMS-PEG copolymer, DOCA-loaded PLGA microspheres, sodium alginate and triethyl citrate, adding a phosphate buffer solution, carrying out vortex oscillation to form homogeneous sol, and filtering and sterilizing to obtain injectable DOCA homogeneous sol; the prepared DOCA homogeneous sol is injected to cynomolgus monkeys according to the dosage of the injection volume of 0.5-1.0 mL / kg; after injection, the cynomolgus monkeys are fed with high-salt diet for 12-13 weeks, and molding is completed. Through the synergistic effect of the innovative DOCA homogeneous sol, the subcutaneous injection method and the high-salt diet, the problems of large animal trauma, high infection risk, long experimental period and the like caused by a traditional administration mode are successfully solved, and a more efficient, stable and accurate technical means is provided for construction of a hypertension model.

The invention discloses a PLGA microsphere-PCL / GelMA composite scaffold based on neurotrophic factor (NGF) slow release as well as a preparation method and application of the PLGA microsphere-PCL / GelMA composite scaffold. According to the composite scaffold, NGF-loaded PLGA microspheres are embedded into a PCL skeleton and a GelMA hydrogel matrix, and a three-phase composite system with mechanical support, biological factor sustained release and cell adhesion functions is constructed. In-vitro experiments show that the NGF-loaded composite scaffold can significantly promote osteogenic differentiation of dental pulp stem cells, which shows that the activity of alkaline phosphatase is improved, the formation of mineralized nodules is increased, and the expression of osteogenic related genes RUNX2, ALPL, COL1A1 and BGLAP is up-regulated. When the composite stent is applied to a rat skull critical defect model in vivo, new bone formation and vascular nerve regeneration are remarkably promoted. Mechanism research shows that regulation and control of osteogenesis and bone resorption are realized by activating a PI3K / AKT signal channel. The composite stent is suitable for repairing complex bone defects in areas with rich nerves and blood vessels, and has a good clinical application prospect.

The application belongs to the field of medicine, and particularly relates to a kind of pirfenidone long-acting controlled-release microspheres and a preparation method thereof.The purpose of the application is to provide a kind of long-acting and accurate controlled-release pirfenidone microspheres, which is prepared by microfluidic method using inner phase solution and outer phase solution.The inner phase solution comprises pirfenidone and its derivatives, PLGA and good solvent of the two;The outer phase solution comprises surfactant, good solvent of the surfactant and inner phase solvent.The pirfenidone long-acting controlled-release microspheres of the application are very uniform by using microfluidic preparation of PLGA microspheres, can smoothly release drug, have high stability, repeatability and regularity, the microfluidic technology for preparing pirfenidone-PLGA sustained-release microspheres proposed in the application is very promising in combination with customization strategy, and the related microsphere product is also beneficial to transformation, and is an effective solution.