17 results about "Plga microspheres" patented technology

The invention discloses composite hydrogel bio-ink with a function of space-time delivery of double growth factors as well as a preparation method and application of the composite hydrogel bio-ink, and belongs to the technical field of biomedical materials and tissue engineering. According to the preparation method, imidazolyl-modified methacrylated gelatin and double-bonded PF127 are compounded, and meanwhile, free vascular endothelial growth factors and connective tissue growth factor-loaded PLGA microspheres are entrapped in a system, so that the bio-ink with excellent printability, high adhesion, swelling resistance and space-time delivery function is formed; the bio-ink physically has the advantages of high printing fidelity, low swelling property, high mechanical strength and strong tissue adhesion of a synthetic material; in biology, the system can be used as an intelligent carrier to realize programmed space-time delivery of various growth factors, so that complex cell biological behaviors are accurately arranged, and real regeneration of functional tissues is guided.

The invention relates to a spinal cord injury repair method based on four-dimensional multifunctional hydrogel, which comprises the following steps: firstly, encapsulating NT-3 in PLGA (poly (lactic-co-glycolic acid)) microspheres through a microsphere technology, and integrating HCT (Hydroxycycline Computed Tomography) into SilMA hydrogel, so as to construct four-dimensional multifunctional hydrogel 4DMSH with time specificity; the hydrogel shows a remarkable anti-inflammatory effect in an in-vivo experiment, can effectively inhibit the inflammatory reaction at the early stage of spinal cord injury, promotes eNSCs to differentiate into neurons by improving a microenvironment and inhibits formation of glial scars, so that the nerve repair efficiency is improved; the four-dimensional multifunctional hydrogel has time specificity and can create an anti-inflammatory and neuroactive microenvironment, so that effective spinal cord injury repair and nerve regeneration are realized; the hydrogel can be widely applied to SCI treatment, and a brand new strategy is provided for repairing nervous system injury.

This invention discloses a microsphere-oil gel composite sustained-release injection and its preparation method. The method includes the following steps: dissolving PVA in pure water to obtain an aqueous phase; dissolving PLGA in an organic solvent to form an organic phase; adding vitamin B12 in solid form to the organic phase to form a solid-oil phase suspension system; adding the solid-oil phase suspension system to the aqueous phase for emulsification to form a solid-oil-aqueous emulsion; adding the emulsion to excess pure water to form drug-loaded PLGA microspheres and freeze-drying; using peanut oil as the oil phase, heating to 60-65°C, adding a gelling agent (a mixture of glyceryl monostearate and beeswax) to the oil phase to form a clear and homogeneous oil phase system; cooling the oil phase system to 30-40°C, adding drug-loaded PLGA microspheres, and uniformly dispersing them under stirring conditions, then continuing to cool naturally to room temperature to obtain the microsphere-oil gel composite sustained-release injection.

The invention discloses a preparation method of multifunctional microspheres loaded with curcumin-hydroxyapatite-PLGA (poly (lactic-co-glycolic acid)), which comprises the following steps: step 1, weighing raw material medicines and PLGA in a beaker, adding an organic solvent into the raw material medicines and the PLGA, and carrying out ultrasonic treatment until the raw material medicines and the PLGA are completely dissolved to form a transparent oil-phase solution; wherein the raw material medicines comprise curcumin and hydroxyapatite; adding ultrapure water into the weighed polyvinyl alcohol emulsifier, and stirring until the emulsifier is completely dissolved to form a water phase solution; step 2, dropwise adding the organic phase into the aqueous phase solution, and carrying out continuous stirring and ultrasonic treatment, so that the organic phase is uniformly dispersed in the aqueous phase to form primary emulsion; 3, stirring the obtained primary emulsion, and removing the organic solvent in the primary emulsion; and centrifuging, washing and precipitating the primary emulsion without the organic solvent, removing the free emulsifier and the unencapsulated medicine, and carrying out vacuum drying to obtain the Cur-HA / PLGA microspheres. The curcumin and the nano-hydroxyapatite can be jointly loaded on the PLGA microspheres, so that the antibacterial, anti-inflammatory and bone regeneration promoting synergistic effect is realized.

The application discloses a medical dressing for wound repair and a preparation method thereof. The dressing comprises a gel matrix and modified growth factor-encapsulated PLGA microspheres. The modified growth factor-encapsulated PLGA microspheres are growth factor-encapsulated PLGA microspheres modified with alkali lignin on the surface. The modified growth factor-encapsulated PLGA microspheres can effectively prevent ultraviolet damage of growth factors and improve bioavailability. Meanwhile, the modified growth factor-encapsulated PLGA microspheres have a suitable degradation period, can release growth factors to promote cell proliferation and wound healing in the proliferation period of a wound, and are stably dispersed in an aqueous phase, so that the dispersion uniformity of the modified growth factor-encapsulated PLGA microspheres in the dressing is improved, and batch stability of the dressing is improved.

The invention discloses a medical dressing for wound repair and a preparation method thereof. The dressing comprises a gel matrix and modified PLGA (poly (lactic-co-glycolic acid)) microspheres for encapsulating growth factors, the modified PLGA microspheres for encapsulating the growth factors are PLGA microspheres for encapsulating the growth factors, and the surfaces of the PLGA microspheres are modified with alkali lignin. The modified PLGA microspheres encapsulating the growth factors can effectively prevent ultraviolet damage of the growth factors, and the bioavailability is improved; meanwhile, an appropriate degradation period is achieved, and growth factors can be slowly released in the proliferation period of the wound to promote cell proliferation and wound healing; furthermore, the PLGA microspheres with the alkali lignin surface modified and encapsulated with the growth factors are stably dispersed in a water phase, so that the dispersion uniformity of the PLGA microspheres in the dressing is improved, and the batch stability of the dressing is improved.

The invention belongs to the technical field of biological medicine, and particularly relates to a lamivudine prodrug long-acting PLGA microsphere preparation and a preparation method thereof. The lamivudine prodrug long-acting PLGA microsphere comprises a lamivudine fatty acid ester prodrug and a PLGA carrier material, the structural general formula of the lamivudine fatty acid ester prodrug is [lamivudine mother nucleus]-O-CO-(CH2) n-CH3, n is an integer of 4-18, and the lamivudine fatty acid ester prodrug is formed by connecting lamivudine and fatty acid through an amido bond; the microspheres are prepared by adopting a single emulsion solvent evaporation method, and the prepared microspheres have the advantages of high drug loading capacity (5%-11%), high encapsulation efficiency (36%-98%) and low burst release effect (24 h lt) by optimizing a prodrug structure, PLGA material characteristics and process parameters; 10%) and the like, and stable drug release of more than 4 weeks can be realized.

The invention discloses an inner ear local release hydrogel and a preparation method thereof, and belongs to the field of biomedical materials and drug delivery. PLGA microspheres are used as model particles, after the PLGA microspheres are wrapped by polydopamine, a diol structure is introduced to the surface, and the PLGA microspheres and a phenylboronic acid grafted hyaluronic acid main chain form a stable borate bond under a neutral condition, so that a three-dimensional injectable hydrogel network is constructed under the condition that no extra small molecule cross-linking agent is needed. The drug-release hydrogel provided by the invention has good biocompatibility, adhesion and slow release ability, can prolong local drug residence time, improves the drug concentration of inner ears, and significantly improves the treatment effect.

The invention discloses a ziconotide-loaded PLGA (poly (lactic-co-glycolic acid)) sustained-release microsphere as well as a preparation method and application thereof. The PLGA microspheres are mainly prepared from a polylactic acid-glycolic acid copolymer (PLGA), ziconotide and trehalose; the PLGA microspheres are prepared from the following components in percentage by mass: 85.2 to 95.6 percent of PLGA, 4.4 to 8.2 percent of ziconotide and 0 to 7.4 percent of trehalose, ziconotide medicine is uniformly dispersed in PLGA, microspheres are prepared by adopting a double-emulsification method, and stable medicine carrying microspheres are formed. The PLGA microspheres prepared by the invention can realize long-time slow release of ziconotide, significantly prolong the drug effect maintenance time, and show relatively low toxic and side effects in vivo; by means of controllable degradation of the PLGA material, ziconotide can be continuously released in vivo, the bioavailability of the medicine is improved, the compliance burden of a patient is reduced, the defects of an existing ziconotide administration mode are overcome, and an efficient, safe and sustainable slow-release treatment scheme is provided.

The application belongs to the field of biological pharmacy, and relates to a temperature-sensitive hydrogel, in particular to a temperature-sensitive hydrogel SV@HP based on hyaluronic acid and a preparation method and application thereof; the temperature-sensitive hydrogel SV@HP is used for loading CS-siRNA nanoparticles and VEGF@PLGA microspheres, and is used for realizing microenvironment-responsive drug delivery to treat intrauterine adhesion. The application uses CS-siRNA nanoparticles of chlorogenic acid and TGFbeta1 siRNA self-assembled through a disulfide bond, is used for gene and chemotherapy to treat intrauterine adhesion, and proves to have a good effect of improving intrauterine adhesion through cell and animal experiments. Through evaluation by real-time fluorescent quantification, Western blotting, a cell scratch test, laser confocal microscope observation, inverted fluorescence microscope observation and in vivo experiments, it is found that the SV@HP gel has the effects of anti-inflammation, improvement of endometrial fibrosis, promotion of endometrial angiogenesis and repair of IUA rats, inhibition of intrauterine adhesion and improvement of intrauterine environment.

A spinal cord injury repair method based on biphase fibroin and methacryloyl hydrogel comprises the following steps: firstly, encapsulating NT-3 and NSCs in PLGA microspheres through a microsphere technology, and integrating Ang-(1-7) into SilMA hydrogel, so as to construct the biphase SilMA multifunctional hydrogel (DPSH) with time-specific drug controlled release. The hydrogel shows a remarkable anti-inflammatory effect in an in-vivo experiment, can effectively inhibit the inflammatory reaction at the early stage of spinal cord injury, promotes NSCs to be differentiated into neurons by improving a microenvironment and inhibits formation of glial scars, so that the nerve repair efficiency is improved.

The invention belongs to the technical field of biomedical materials, and provides an intelligent hydrogel dressing with a three-layer structure as well as a preparation method and application of the intelligent hydrogel dressing. The intelligent hydrogel dressing with the three-layer structure comprises an upper layer, a middle layer and a bottom layer which are sequentially stacked, the upper layer is methacrylated gelatin hydrogel containing silver nanoparticles, the middle layer is methacrylated gelatin hydrogel containing mangiferin-loaded PLGA microspheres, and the bottom layer is methacrylated gelatin hydrogel. The upper layer of the intelligent hydrogel dressing with the three-layer structure is used for broad-spectrum antibiosis in the initial stage and quickly coping with bacterial infection; the middle layer is used for continuously releasing mangiferin, exerting the effects of resisting oxidation, resisting inflammation and promoting angiogenesis and eliminating oxidative stress; the bottom layer provides mechanical support and biocompatibility; through the synergistic effect of the multi-layer structure of the upper layer, the middle layer and the bottom layer, remarkable advantages are shown in multiple aspects such as mechanical support, medicine controllable release, broad-spectrum antibiosis and inflammation-oxidative stress regulation, and layering and cooperative intervention are achieved.

This invention discloses a multi-microsphere-coated drug carrier, its preparation method, and its application. The drug carrier uses drug-loaded attapulgite particles as a core, with the core surface coated by interconnected PLGA microspheres. The surface of the drug carrier is uneven and porous. The preparation method of the drug carrier involves: obtaining attapulgite particles through spray granulation, loading the drug into the attapulgite particles, and then coating the attapulgite particles with PLGA. The drug carrier provided by this invention exhibits good sustained-release performance and safety, and is of great significance for antitumor drug delivery.

The invention belongs to the field of medicines, and particularly relates to a perampanel long-acting controlled-release microsphere and a preparation method thereof. The invention aims to provide a long-acting and precise controlled-release perampanel microsphere which is prepared from an internal phase solution and an external phase solution by adopting a microfluidic method, wherein the internal phase solution comprises perampanel and a derivative thereof, PLGA (poly (lactic-co-glycolic acid)) and a good solvent of the perampanel and the derivative thereof; the external phase solution comprises a surfactant, a good solvent of the surfactant, and an internal phase solvent. According to the perampanel long-acting sustained and controlled release microspheres, the PLGA microspheres prepared through microfluidics are very uniform, can stably release drugs and have highly-stable repeatability and regularity, the perampanel-PLGA sustained release microspheres prepared through the microfluidics technology are combined with a customization strategy to be very promising, related microsphere products are also beneficial to transformation, and the perampanel long-acting sustained and controlled release microspheres are suitable for industrial production. The method is an effective solution.

The invention provides temperature-controlled drug-loading hydrogel and a preparation method and application thereof, the temperature-controlled drug-loading hydrogel comprises Dexp-coated PLGA and temperature-sensitive hydrogel, and the mass volume ratio of the Dexp-coated PLGA to the temperature-sensitive hydrogel is (10-50) mg: 1 mL; the Dexp-coated PLGA is a PLGA microsphere loaded with dexamethasone sodium phosphate, and the temperature-sensitive hydrogel is prepared from poloxamer and polycarbophil. Poloxamer and polycarbophil are adopted as temperature-sensitive hydrogel, and matched with Dexp-PLGA composed of dexamethasone sodium phosphate (Dexp) entrapped by PLGA microspheres to form temperature-control drug-loading hydrogel (Dexp-PLGA-Gel), the temperature-control drug-loading hydrogel can be automatically degraded, is not left in vivo, reduces toxic and side effects, is in a liquid state at the temperature of 4 DEG C or normal temperature, is in a gel state at the body temperature of 36 DEG C, and is free of toxic and side effects. The medicine can stay in the middle ear for a long time to play a role, the injection frequency is reduced, and the pain of a patient is relieved.

The application belongs to the field of medicine, and particularly relates to a kind of pirfenidone long-acting controlled-release microspheres and a preparation method thereof.The purpose of the application is to provide a kind of long-acting and accurate controlled-release pirfenidone microspheres, which is prepared by microfluidic method using inner phase solution and outer phase solution.The inner phase solution comprises pirfenidone and its derivatives, PLGA and good solvent of the two;The outer phase solution comprises surfactant, good solvent of the surfactant and inner phase solvent.The pirfenidone long-acting controlled-release microspheres of the application are very uniform by using microfluidic preparation of PLGA microspheres, can smoothly release drug, have high stability, repeatability and regularity, the microfluidic technology for preparing pirfenidone-PLGA sustained-release microspheres proposed in the application is very promising in combination with customization strategy, and the related microsphere product is also beneficial to transformation, and is an effective solution.

The application belongs to the technical field of biomedicine, and specifically discloses a kind of enabled mesenchymal stem cells, enabled mesenchymal stem cell composition and its application in preparation treatment organ dysfunction drug, and the enabled mesenchymal stem cells are obtained by treating human mesenchymal stem cells with a kind of halogenated salicylic alcohol HEP14, and the enabled mesenchymal stem cell composition includes the enabled mesenchymal stem cells and HEP14 / PLGA microspheres with high HEP14 load and long-acting HEP14 sustained release prepared by using polylactic acid-hydroxyacetic acid copolymer (PLGA) as a carrier.The enabled mesenchymal stem cells of the application have the effects of strengthening mesenchymal stem cell survival viability and stemness, resisting tissue fibrosis, promoting tissue angiogenesis, promoting tissue regeneration and functional recovery;the combined application of the enabled mesenchymal stem cells and HEP14 / PLGA microspheres strengthens the retention and transdifferentiation of the enabled mesenchymal stem cells in the tissue, so the function of the enabled mesenchymal stem cells and the application in the field of regenerative medicine are further strengthened.