KGN-carried collagen/chitosan/sodium hyaluronate composite scaffold

A sodium hyaluronate and composite scaffold technology, applied in the field of biomedicine, can solve the problems of adverse reactions, inability to delay or stop the OA process, high price, etc., achieve good biocompatibility, improve the mechanical properties of collagen, and the preparation process simple effect

Inactive Publication Date: 2017-05-31
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most effective way to treat OA clinically is joint replacement, but this method may cause many adverse reactions and is expensive, so it is generally only used in advanced OA patients
At present,

Method used

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  • KGN-carried collagen/chitosan/sodium hyaluronate composite scaffold
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  • KGN-carried collagen/chitosan/sodium hyaluronate composite scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Accurately weigh 200 mg of PLGA and dissolve in 4 mL of dichloromethane, and shake to form an oil phase (O);

[0034] (2) Accurately weigh 10 mg KGN and place it in 1 mL of a mixture of ultrapure water and methanol, and dissolve it completely at 37°C to form an inner water phase (W1); the volume ratio of ultrapure water to methanol is 1 :1;

[0035] (3) Slowly inject the inner water phase (W1) in step (2) into the oil phase (O) in step (1) and continue to stir and emulsify for 10 min, so that it can be evenly dispersed to form a colostrum emulsion (W1 / O);

[0036] (4) Drop the colostrum emulsion (W1 / O) described in step (3) into 50 mL of 1wt% PVA solution containing 5 mL of 0.1wt% Tween-20 at 60 drops / min to form the external water phase (W2), Continuous stirring to form double milk (W1 / O / W2);

[0037] (5) Fully centrifuge the double emulsion (W1 / O / W2) described in step (4), discard the supernatant, wash and centrifuge with ultrapure water repeatedly for 3 times, ...

Embodiment 2

[0045] In step (6) of Example 1, change bovine tendon collagen to fish skin collagen; in step (10), change the volume ratio of 0.6% COL, 1% CS and 1% HAS solution to 5:10 : 10, the other steps are the same as in Example 1, preparing the collagen / chitosan / sodium hyaluronate composite scaffold loaded with KGN.

Embodiment 3

[0047] In step (6) of Example 1, change bovine tendon collagen to pig skin collagen; in step (10), change the volume ratio of 0.6% COL, 1% CS and 1% HAS solution to 1:10 : 10, the other steps are the same as in Example 1, preparing the collagen / chitosan / sodium hyaluronate composite scaffold loaded with KGN.

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PUM

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Abstract

The invention discloses a KGN-carried collagen/chitosan/sodium hyaluronate composite scaffold. The scaffold is prepared from collagen, chitosan, sodium hyaluronate, KGN (kartogenin) and PLGA (poly(lactic-co-glycolic acid)). The preparation method comprises the following steps: preparing KGN-micromolecule-carried PLGA microspheres, mixing the PLGA microspheres, and preparing the collagen/chitosan/sodium hyaluronate composite scaffold. The composite scaffold has the characteristics of favorable biocompatibility, favorable biodegradability and the like, has the lubrication action of sodium hyaluronate and the marrow mesenchymal stem cell cartilage differentiation promotion action of KGN, is beneficial to cartilage defect repair of medium/early osteoarthritis, and is a favorable tissue regeneration material for osteoarthritis cartilage defects.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a collagen / chitosan / sodium hyaluronate composite bracket loaded with KGN. Background technique [0002] In recent years, with the acceleration of population aging, more and more people are troubled by articular cartilage damage, especially patients with osteoarthritis (Osteoarthritis, OA). OA is a multifactorial degenerative disease characterized by degeneration of articular cartilage, reactive hyperplasia of joint margins and subchondral bone, clinically manifested as slowly developing joint pain, tenderness, stiffness, joint swelling, limited mobility and joint deformity etc. The most effective method to treat OA clinically is joint replacement, but this method may cause many adverse reactions and is expensive, so it is generally only used in advanced OA patients. At present, there is no effective treatment for patients with early and middle stages of OA, and most metho...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/20A61L27/18A61L27/54A61L27/58C08J3/24
CPCA61L27/24A61L27/18A61L27/20A61L27/54A61L27/58A61L2300/412A61L2300/602A61L2430/06C08J3/246C08J2305/04C08J2305/08C08J2489/00C08L5/08C08L67/04
Inventor 王建华张其清孙晓敏张志华王营营
Owner FUZHOU UNIV
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