164 results about "Double emulsion" patented technology

The invention relates to the technical field of medicine, and discloses an artificial exosome and application thereof in drug delivery, the artificial exosome is prepared into an oil phase based on PLGA and DOTAP, and a water phase based on OCT4 protein and siTrim28; mixing the two phases to obtain a primary emulsion, and adding a PVA aqueous solution to obtain a double emulsion; stirring the double emulsion and volatilizing the solvent to form an emulsified solution, and centrifuging to obtain PLGA nanoparticles; the lipid mixture is prepared from lipid, DPPE-PEG2000, alpha-tocopherol and alpha-lactalbumin, and a lipid film is formed after a solvent is evaporated; the preparation method comprises the following steps: mixing PLGA nanoparticles, a lipid film and membrane protein, uniformly mixing and heating to obtain a film-coated solution, and finally extruding through a cellulose acetate film to obtain the artificial exosome. The artificial exosome disclosed by the invention combines the stability of the PLGA nanoparticles and the biocompatibility of the lipid membrane, and provides new possibility for a drug delivery system.

The invention belongs to the technical field of cosmetics, and discloses skin-tightening and anti-wrinkle essence. The skin-tightening anti-wrinkle essence is prepared from the following raw materials in percentage by mass: 1.5 to 2.5 percent of acetyl hexapeptide-8, 2.0 to 3.0 percent of palmitoyl pentapeptide-4, 3.0 percent of hydroxypropyl tetrahydropyrantriol, 2.0 percent of lipidosome-coated vitamin C ethyl ether, 0.5 percent of resveratrol PLGA microspheres, 0.8 percent of ceramide NP, 1.2 percent of pachymaran, 5.0 percent of glyceryl polyether-26, 0.3 percent of sodium hyaluronate, 0.5 percent of p-hydroxyacetophenone and 1, 3-propanediol, 0.5 percent of sodium hyaluronate, 0.5 percent of sodium hyaluronate, 0.5 percent of sodium hyaluronate and the balance of water. The 1, 2-hexanediol accounts for 0.6%, and the balance is deionized water. Multi-target anti-wrinkle, deep moisturizing and long-acting repairing are achieved by scientifically compounding active ingredients such as acetyl hexapeptide-8 and palmitoyl pentapeptide-4 and combining a lipidosome wrapping technology and a nano-microsphere slow release technology, the preparation process comprises the steps of lipidosome homogenization, nano-microsphere multiple emulsion method and low-temperature emulsification, the ingredient stability and transdermal efficiency are ensured, and the skin care effect is good. Experiments prove that the essence can significantly improve skin elasticity and reduce wrinkles, and has a significant anti-aging effect.

The invention relates to the technical field of functional composite materials, and discloses a preparation method of polylactic acid porous microspheres and application of the polylactic acid porous microspheres in stem cell inoculation, and the preparation method comprises the following steps: (1) dissolving chitosan in an acetic acid aqueous solution, adding bioactive glass nanoparticles, and performing ultrasonic treatment to obtain an aqueous phase solution; (2) dissolving PLGA or PLA in dichloromethane, and adding an emulsifier to obtain an oil phase solution; (3) dropwise adding the water-phase solution into the oil-phase solution to obtain a water-in-oil primary emulsion; (4) dropwise adding the water-in-oil primary emulsion into a PVA aqueous solution to obtain a double emulsion; and (5) stirring the double emulsions, carrying out centrifugal separation, drying, and immersing in a glutaraldehyde solution for cross-linking treatment to obtain the porous microspheres. The porous microspheres are prepared by a double-emulsion solvent evaporation method, and have more uniform pore size distribution and higher biological activity, so that the porous microspheres can be used as an excellent stem cell inoculation carrier to meet the attachment and proliferation of high-proportion cells.

The invention discloses a dual-mode micro-droplet generation micro-fluidic chip and application thereof in generation of dual-emulsion droplets, and relates to the technical field of micro-fluidic chips. The method comprises the following steps: respectively injecting two different internal phase solutions from the left ends of respective iron tubes, guiding the internal phase solutions to a wedge-shaped tube orifice of a theta-shaped glass capillary tube through respective thin tubes, injecting an intermediate phase solution into a square glass tube through an intermediate phase inlet by using a No.1 needle, and injecting an external phase solution into the square glass tube through an external phase inlet by using a No.2 needle; the two internal phase solutions, the intermediate phase solution and the external phase solution converge at an inlet of the circular glass capillary tube, and after entering the circular glass capillary tube, water-in-oil-in-water double-emulsion droplets are obtained. The invention can obtain the dual-mode micro-droplet generation micro-fluidic chip and the application of the dual-mode micro-droplet generation micro-fluidic chip in generation of dual-emulsion droplets.

The invention discloses a preparation method and application of a targeted delivery system of a metal polyphenol nano system. The invention discloses a targeted delivery system of a metal polyphenol nano system, and a preparation method of the targeted delivery system comprises the following steps: taking the metal polyphenol nano system formed based on epigallocatechin gallate and iron ions as a carrier, and loading fucoxanthin to prepare fucoxanthin nano particles with active oxygen response characteristics; then, after chondroitin sulfate capable of targeting macrophages and (5-carboxyl amyl) triphenyl phosphorus bromide capable of targeting mitochondria are coupled, the fucoxanthin delivery system with the active oxygen response characteristic and the targeting characteristic is further prepared through a double emulsion anti-solvent evaporation method. The system can realize release of fucoxanthin under stimulation of active oxygen, and realizes aggregation of a targeted delivery system in mitochondria of a specific inflammation site. The carrying system can effectively improve or relieve the inflammatory bowel disease, and the raw materials used in the carrying system are low in cost, the preparation conditions are mild, and large-scale production is easy.

The invention provides a Ziegler-Natta catalyst silicon-magnesium composite carrier and a preparation method thereof, a continuous phase silicon solution is prepared by using a double template agent, the continuous phase silicon solution is mixed with a cross-linking agent and an initiator to obtain a dispersed phase, the dispersed phase is subjected to two-phase speed regulation mixing through a micro-fluidic chip to finally obtain double emulsion droplets, and then operations such as curing, template agent removal and acid etching are performed to obtain the Ziegler-Natta catalyst silicon-magnesium composite carrier. The preparation method comprises the following steps: preparing a spherical silicon dioxide carrier with more excellent performance, then carrying out magnesium source loading and post-treatment, and finally preparing the Ziegler-Natta catalyst silicon-magnesium composite carrier with monodispersity, hierarchical porous structure (micropore-mesopore-macropore), high loading rate and high mechanical strength, and is suitable for industrial-scale polypropylene catalyst production. The prepared catalyst carrier is more perfect in morphology and more uniform in particle size.

The invention provides tranexamic acid-glutathione-containing compound emulsifiable paste and a preparation method thereof.W1 / O / W double emulsion phase structural design is adopted, reduced glutathione is encapsulated in an acidic inner water core, an oil phase is stabilized through hydrogenated lecithin and sorbitan sesquioleate, an outer water phase contains tranexamic acid, a layered gel network is constructed, and the tranexamic acid-glutathione-containing compound emulsifiable paste is prepared. A submicron dispersion system with the particle size D50 of 0.30-0.80 microns, the polydispersity index of 0.15-0.25 and the viscosity of 2.0-8.0 Pa seconds is realized, the encapsulation efficiency of glutathione is not lower than 70%, the oxygen content of a head space is not higher than 1.0%, and the encapsulation efficiency of glutathione is not lower than 70%. The three technical contradictions of coupling conflict between a high-yield viscoelastic structure and low-shear damage kernel integrity, coexistence of fresh and cool low-viscosity skin feeling and high-barrier anti-oxidation and anti-leakage and opposite low-surface-activity sensitive skin compatibility and submicron low-PDI dispersion stability are solved; the traditional Chinese medicine composition has wide application values of improving skin color unevenness, fading color spots, brightening skin color, soothing and repairing.

The present invention relates to a method for preparing solid microcapsules comprising the following steps:a) preparation of a composition C1, which is either a composition C1a, comprising a single hydrophobic solid particle, or a composition C1b comprising a plurality of hydrophobic solid particles dispersed in a hydrophilic phase,b) addition under stirring of the composition C1 in a polymeric composition C2 at a temperature Tb, whereby an emulsion (E1) is obtained;c) addition, under stirring, of the emulsion (E1) in a composition C3 at a temperature Tc, whereby a double emulsion (E2) is obtained;d) applying a shear to the emulsion (E2), whereby a double emulsion (E3) is obtained; ande) the polymerization of the composition C2, whereby solid microcapsules dispersed in the composition C3 are obtained.

The invention discloses a self-assembled organic material crystal incubation method based on double-emulsion droplets, which comprises the following steps: preparing W / O / W type double-emulsion droplets with uniform size through a micro-fluidic chip, in the preparation process of the double-emulsion droplets, taking a self-assembled organic material solution as an inner water phase solution, taking an intermediate oil phase solution as a water-insoluble solution, and taking a micro-fluidic chip as an outer water phase solution; a self-assembled organic material solution is packaged by using an external water phase solution with higher salt concentration, so that the self-assembled organic material is prevented from being dissociated into the external water phase solution, and double emulsion drops loaded with the self-assembled organic material solution are incubated. By utilizing the concentration difference between the external water phase solution and the internal water phase solution, under the influence of osmotic pressure, water molecules in the internal water phase solution migrate to the external water phase, and the concentration of the self-assembled organic material in the double-emulsion liquid drops is also increased, so that the growth of the self-assembled organic material crystal is promoted; finally, self-assembled organic material crystals with uniform sizes are obtained in the double-emulsion droplets, and each double-emulsion droplet contains a single self-assembled organic material crystal.

The invention discloses a preparation method of a double-emulsion microcapsule for improving the light stability of vitamin B12 in soft sweets. By constructing a double emulsion system, seepage of the vitamin B12 is effectively prevented, meanwhile, solid fat in an oil phase can effectively block damage of external ultraviolet rays to the vitamin B12, and the storage stability of the vitamin B12 is remarkably improved. In addition, the microcapsule is easy to disperse in water due to the selected water-soluble outer-layer wall material, so that the preparation of the soft sweets is facilitated. And the preparation process is simple and easy for large-scale production. The encapsulation efficiency of the vitamin B12 microcapsule prepared by the method is more than 65%. And the retention rate of the vitamin B12 can reach more than 60% after the vitamin B12 is stored for 28 days under a room-temperature illumination condition, which is obviously superior to the retention rate of the raw materials.

The present invention discloses a method for preparing Lactobacillus casei and a preparation thereof. The Lactobacillus casei was deposited in the China General Microbiological Culture Collection Center on July 6, 2022, with a deposit number of CGMCC No. 25194. The preparation method of the double-layer emulsion probiotic preparation comprises the following steps: (1) vortexing Lactobacillus casei and a probiotic protective agent solution to uniformly disperse them to form an inner water phase W1; (2) dissolving ursolic acid in oil to form an oil phase O, and mixing the oil phase O with the inner water phase W1 by high-speed shearing to obtain an oil-in-water type colostrum W1 / O; (3) using a suspension of colloidal particles as an external water phase W2, adding the external water phase W2 to the colostrum W1 / O, and mixing by medium-speed shearing to obtain a double-layer emulsion probiotic preparation with a W1 / O / W2 type double emulsion structure.

The invention provides a double-emulsification high-flux micro-fluidic chip and a preparation method thereof. The chip comprises a substrate, a middle layer and a cover plate. According to the invention, the substrate is not processed, but the main micro-channels are processed at the two sides of the middle layer, and the cover plate is punched, so that the fluid can be conveniently introduced. A plurality of liquid drop generation units based on the flow focusing technology are designed on one side of the middle layer so as to generate liquid drops for the first time, and a stepped liquid drop generation structure based on interfacial tension is designed on the other side of the middle layer so as to generate liquid drops for the second time. The structures on the two sides of the chip are connected through the through holes. The preparation method comprises the following steps: carrying out photoetching to form a mold, preparing the middle layer by using the mold, forming the required micro-channels on the two sides of the middle layer, punching on the cover plate, and finally bonding the substrate, the middle layer and the cover plate. Through the design of the flow channel and the structure, a large number of double-emulsion droplets can be rapidly prepared, the production efficiency is improved, and the device is suitable for various applications such as drug delivery and biological research.

The invention discloses multi-response microgel with an ultrathin shell layer and a preparation method of the multi-response microgel, and relates to the technical field of gel. The preparation method comprises the following steps: preparing an inner oil phase, a middle water phase and an outer oil phase; respectively injecting the inner oil phase, the intermediate water phase and the outer oil phase into a microfluidic device to obtain O / G / O type double emulsion microgel; and carrying out a cross-linking reaction on the double emulsion and a cross-linking agent, thereby obtaining the product. According to the preparation method disclosed by the invention, the O / G / O type double-emulsion microgel with a core-shell structure is successfully prepared by taking corn oil, gelatin and sodium alginate as main raw materials by adopting a micro-fluidic technology, so that the embedding rate of a target object can be improved, and active adsorption and embedding of active substances are realized; the emulsion microgel is further subjected to cross-linking modification, through cross-linking modification, the stability and strength of the O / G / O type double emulsion microgel structure after swelling adsorption are improved, the shell layer of the microgel is converted into a nano-scale thin film from a micron-scale thin film, and the multi-response microgel with an ultrathin shell layer is formed.

The invention relates to the technical field of colloid and interface chemistry, in particular to an oil-in-water-in-oil double emulsion, a one-step preparation method of the oil-in-water-in-oil double emulsion and application of the oil-in-water-in-oil double emulsion. A water phase and an oil phase are sheared and stirred at a high speed of 6000-12000 r / min through a one-step process to form an emulsion with a droplet diameter of 1-100 [mu] m. According to the invention, the tedious process of respectively stabilizing the inner and outer oil-water interfaces of the double emulsion through two emulsifiers with different hydrophilic and oleophylic properties in a traditional two-step method is effectively improved, and the O / W / O double emulsion is directly obtained through a one-step process, so that the purpose of simultaneously stabilizing the inner and outer emulsion interfaces of the double emulsion through a single emulsifier is achieved; the method has a huge potential application prospect in the fields of food packaging, drug sustained release and the like.

The invention discloses a double emulsion generation system and a generation method, and belongs to the technical field of microfluidics. Comprising a liquid storage device, a pressure pumping device, a nested micro-fluidic chip and a double emulsion collecting tank, the liquid storage device is used for storing an internal phase fluid and an intermediate phase fluid, the pressure pumping device is communicated with the liquid storage device, and the liquid storage device is communicated with the nested micro-fluidic chip; a micro-channel is arranged in the nested micro-fluidic chip, and the micro-channel is of a micro-step structure. And the dual emulsion collecting tank is communicated with the nested micro-fluidic chip. The method does not involve micro-channel local surface modification, solves the problems of complex and unstable micro-channel local surface modification in the conventional double emulsion generation process, and greatly improves the production flux and production stability of the double emulsion.

The application discloses an incubation method of self-assembled organic material crystals based on double emulsion drops, and the method comprises the following steps: preparing W / O / W type double emulsion drops with uniform size through a microfluidic chip; during the preparation of the double emulsion drops, using a self-assembled organic material solution as an inner water phase solution, using a non-water-soluble solution as an intermediate oil phase solution, and using a water phase solution containing a high salt concentration as an outer water phase solution to encapsulate the self-assembled organic material solution, so as to avoid the self-assembled organic material from being free in the outer water phase solution; and incubating the double emulsion drops loaded with the self-assembled organic material solution. By using the concentration difference between the outer water phase solution and the inner water phase solution, under the influence of osmotic pressure, water molecules in the inner water phase solution migrate to the outer water phase, and the concentration of the self-assembled organic material in the double emulsion drops also increases, so as to promote the growth of the self-assembled organic material crystals, and finally, the self-assembled organic material crystals with uniform size are obtained in the double emulsion drops, and each double emulsion drop contains a single self-assembled organic material crystal.

The present invention relates to a food composition using a natural extract for enhancing saltiness. More specifically, a mixing ratio of a shiitake mushroom extract, a lotus root extract and a Chinese cabbage extract, which have high saltiness perceivability, is provided and is used for preparing a double emulsion. The double emulsion prepared according to the present invention has enhanced saltiness while having a stable form, and enables the amount of oil to be reduced, and thus the present invention can be applied to the preparation of various food by using the emulsion.

The invention discloses a water-in-oil-in-water type composite emulsion and a preparation method thereof, the composite emulsion has a W1 / O / W2 double emulsion structure, W1 is an inner water phase, O is an oil phase, W2 is an outer water phase, the outer water phase is a protein-polysaccharide compound, the inner water phase is prepared from a chlorogenic acid aqueous solution, and the outer water phase is prepared from a chlorogenic acid aqueous solution. The oil phase is prepared from soybean oil and polyglycerol ricinoleate. The emulsion is prepared from bovine whey protein and high-ester pectin in an outer water phase, the encapsulation efficiency of chlorogenic acid is improved, the embedding rate of the water-in-oil-in-water type chlorogenic acid composite emulsion with the volume ratio of a bovine whey protein powder solution to a high-ester pectin powder solution being 1: 5 is the highest and is 94.52%, and the scavenging activity on DPPH free radicals is 1.59 times that of isoconcentration chlorogenic acid monomers. Meanwhile, erosion of gastric acid can be more effectively resisted in vivo, so that high-content chlorogenic acid reaches the intestinal tract to be colonized, and the anti-inflammatory activity is achieved.

This invention discloses a method for preparing hydrophilic drug nanoliposomes based on microfluidic-low-shear coupling double emulsion and the hydrophilic drug nanoliposomes prepared according to the following method: (1) Prepare aqueous solutions of hydrophilic drugs and chloroform solutions of soybean lecithin respectively; (2) Add the chloroform solution of soybean lecithin to the chamber as the oil phase, and inject the aqueous solution of hydrophilic drugs into the oil phase in the low-shear stirring field through a microtube under low-speed stirring and shearing, and collect the W / O pre-emulsion sample; (3) Use the W / O pre-emulsion sample as the dispersed phase, and inject it into the external aqueous phase in the same way as in step (2), and emulsify it under low-speed stirring and shearing to form a W / O / W double emulsion; (4) Remove the chloroform by vacuum evaporation of the W / O / W double emulsion to obtain hydrophilic drug nanoliposomes. The prepared Ganoderma lucidum polysaccharide nanoliposomes have a particle size controlled at 200-300 nm, a polydispersity index of about 0.2, and an encapsulation efficiency of more than 70%, which is more than twice that of the traditional thin film hydration method. It solves the technical problem of low encapsulation efficiency of water-soluble macromolecular Ganoderma lucidum polysaccharides.

The present invention discloses a method for preparing cationic hyaluronic acid microspheres, as well as the resulting products and applications. The method prepares hyaluronic acid polyester microspheres by a water / oil / water double emulsion solvent volatilization method, then introduces amino groups onto the microsphere surfaces through an aminolysis reaction to construct cationic hyaluronic acid microspheres. The microspheres are then applied to moisturizing finishing of textile fabrics. The cations of the microspheres and the anions on the surface of the fabric fibers form an electrostatic force, strengthening the bonding between the microspheres and the fabric, improving the washability of hyaluronic acid textiles, and resolving the problem of hyaluronic acid textiles being unable to retain moisture over the long term.

The application belongs to the field of food functional factor processing, and particularly relates to a water-in-oil-in-water composite emulsion embedding lactoferrin, a preparation method and application. The W1 / O / W2 double emulsion system is constructed by a two-step method, and the leakage and inactivation risks of lactoferrin and VD3 in the preparation, storage and processing processes are effectively reduced through the double barrier effect of the oil phase film and the outer water phase film. Under the optimized sodium caseinate and gellan gum compounding conditions, the encapsulation rate of lactoferrin can be up to 82.52%, which proves that the structure has obvious advantages in improving the loading efficiency of active substances, and can significantly improve the encapsulation rate and structural stability of lactoferrin. The water-in-oil-in-water composite emulsion system prepared by the application can significantly improve the retention rate of lactoferrin in the digestion process, enhance the tolerance of lactoferrin to the gastrointestinal environment, and then enhance the arrival rate of lactoferrin in the intestinal tract, thereby improving the overall bioavailability.

The invention relates to an aroma-carrying heating cigarette and a preparation method thereof, the aroma-carrying heating cigarette comprises a heating cigarette body and an O1 / W / O2 type aroma-carrying dual emulsion, and the O1 / W / O2 type aroma-carrying dual emulsion is loaded on a filter tip section and / or a cooling section of the heating cigarette body. According to the invention, the essence is loaded by adopting the O1 / W / O2 type fragrance-loading dual emulsion, and the obtained emulsion is relatively high in stability and has a slow-release effect; when the emulsion is loaded on the filter tip section and / or the cooling section of the heating cigarette body, the essence retention rate of the heating cigarette in the storage process and the essence transfer rate and release consistency in the smoking process can be effectively improved, and the smoking quality is improved.

The invention discloses a preparation method and application of PLGA (poly (lactic-co-glycolic acid))-curcumin nanoparticles. The invention relates to the technical field of biomedicine, and the preparation method comprises the following steps: taking a polyvinyl alcohol (PVA) aqueous solution as a water phase and a dichloromethane solution of polylactic acid-glycolic acid copolymer (PLGA) and curcumin (Cur) as an oil phase, preparing curcumin-entrapped PLGA nanoparticles (PLGA at Cur NPs) by adopting a water-in-oil-in-water double emulsion method, and the prepared PLGA nanoparticles are spherical, uniform in particle size and uniform in particle size. The carrier PLGA has good biocompatibility and biodegradability and is non-toxic and non-irritant, and a surfactant does not need to be added in the preparation process of the nanoparticles, so that the toxicity is further reduced.

The preparation method of the antimony trioxide with the porous structure comprises the following steps: adding an antimony trioxide matrix into a montmorillonite loading agent which is 5-8 times of the total amount of the antimony trioxide matrix, stirring, carrying out suction filtration, and drying to obtain the antimony trioxide with the porous structure. An antimony trioxide matrix is constructed by adopting a double emulsion template method, meanwhile, montmorillonite is pretreated, interlayer water is damaged by preheating at 210-230 DEG C, then the temperature is increased to 350 DEG C, heat preservation is performed for 1 h, an interlayer structure is stripped through thermal expansion, the specific surface area is increased, more active sites are exposed, and the specific surface area is increased. And dopamine / carbon nanotube composite modification: dopamine hydrochloride is subjected to self-polymerization under a weakly alkaline condition to form a polydopamine (PDA) coating layer.

The present invention discloses a method and device for preparing large-sized microspheres with uniform particle size and regular shape, which relates to the field of emulsion preparation technology, including: preparing a double emulsion microsphere material containing an inner phase, an intermediate phase and an outer phase, wherein the inner phase includes an aqueous solution containing an active ingredient and a hydrophilic polymer surfactant, and the intermediate phase includes a melting point of 50°C. <T 熔 The oil phase and lipophilic nonionic surfactant at a temperature of <85°C, the external phase is an aqueous solution, or a single emulsion microsphere material is prepared; the material is then passed into the microfluidic chip for molding, and the microsphere molding temperature is T 熔 + (10~15) ℃; the formed microspheres are cured by gradient cooling in the liquid outlet tube or the formed microspheres are directly dropped into the receiving liquid for curing. The receiving liquid temperature is (T 熔 ‑30)~(T 熔 ‑5) ℃; the preparation method of the present invention ensures stable microsphere molding, low probability of microsphere demulsification and irregular shape, and the prepared large-sized microspheres have uniform particle size and regular morphology.

This invention belongs to the field of fire extinguishing materials and their preparation technology, and provides a fire extinguishing microcapsule and its microfluidic controllable preparation method. The preparation method is characterized by the following steps: (1) preparing fluids of each phase, (2) preparing a dual emulsion using a microfluidic device, (3) collecting and sealing the dual emulsion in a collection container, controlling the axis of the collection container to be horizontal, and controlling the collection container to rotate around its axis for 1 to 15 minutes, applying light to solidify the outer shell of the dual emulsion droplets while maintaining the rotation of the collection container, cleaning, and drying to obtain the final product. The fire extinguishing microcapsule prepared by this method consists of an outer shell and a liquid fluorine-containing fire extinguishing agent encapsulated within the outer shell. The outer shell of the fire extinguishing microcapsule has a uniform thickness, and the eccentricity between the outer shell and the core is less than 0.1. The method of this invention can achieve precise customization of fire extinguishing microcapsules according to the differentiated fire characteristics of different application scenarios, and can improve the concentricity between the core and the outer shell of existing fire extinguishing microcapsules.

The application relates to the embedding technical field, and particularly discloses a double emulsion gel bead embedding beta-carotene and probiotics and a preparation method thereof. The double emulsion gel bead comprises a composite layer, an oil phase and an inner water phase from outside to inside. The composite layer comprises an outer water phase and calcium alginate which are fused with each other. The outer water phase comprises a product after carrageenan and sodium caseinate are subjected to a Maillard reaction. The oil phase comprises beta-carotene, vegetable oil and a food emulsifier. The inner water phase comprises probiotics. In the gel bead, the beta-carotene and the probiotics are simultaneously embedded in the double emulsion gel bead, the calcium alginate hydrogel shell starts to decompose when entering the intestinal tract, then the double-layer emulsion is slowly decomposed after being subjected to chemical digestion of pancreatic juice, bile and small intestinal juice and mechanical digestion of the small intestine in the small intestine, and the beta-carotene and the probiotics are gradually released, the beta-carotene is converted into vitamin A in the intestinal tract and is absorbed, and the probiotics are delivered to the colon to play a probiotic effect.

Systems and methods for measuring osmotic pressure are described. In many embodiments, double emulsion droplets can be used as osmotic pressure sensors to measure local osmotic pressure. The double emulsion droplets can measure osmotic pressure in living cells and / or tissues.

The invention belongs to the technical field of food processing, and particularly relates to ultrasonic phosphoric acid acetylated mung bean protein as well as a preparation method and application thereof. According to the ultrasonic phosphoric acid acetylated mung bean protein preparation method provided by the invention, the mung bean protein is prepared by utilizing the combined action of three methods of ultrasonic assistance, phosphorylation modification and acetylation modification; the emulsifying activity of the prepared ultrasonic phosphoric acid acetylated mung bean protein reaches 149 mL / g and the emulsifying stability reaches 75%; the double emulsion system prepared on the basis of the ultrasonic phosphate acetylated protein shows the following outstanding properties: the average particle size of liquid drops is as low as 0.6 mu m, the entrapment efficiency is as high as 71%, the liquid drop distribution uniformity is remarkably improved, and meanwhile, the physical stability of the emulsion is effectively enhanced due to the moderate increase of the viscosity of the system.

The present invention belongs to the technical field of pharmaceutical preparations, and specifically relates to a method for preparing a nanocomposite carrying PDGF-BB mRNA, the preparation method comprising: 1) preparing LNP carrying PDGF-BB mRNA: quickly mixing a lipid phase containing 7C1 cationic liposomes with an aqueous phase containing PDGF-BB mRNA to obtain LNPmRNA; 2) preparing a PLGA gas core core by a double emulsion method; 3) mixing the PLGA gas core core in step 2) with the LNPmRNA in step 1), and standing to obtain a LNPmRNA@PLGA nanocomposite. The LNPmRNA@PLGA nanocomposite of the present invention has the advantages of protection, targeting, visibility and responsiveness. It is combined with in vitro focused ultrasound, which can not only realize the visual delivery of drugs, but also qualitatively and semi-quantitatively evaluate the neovascularization in advanced atherosclerotic plaques, and improve the cell transfection efficiency under the targeted microbubble destruction effect of in vitro focused ultrasound, accurately and efficiently overexpress the target protein, achieve the goal of drug treatment, and reduce systemic toxic side effects.