Preparation method of ferric oxide nanoparticle supported sodium alginate nanogel

A technology of iron oxide nanometers and sodium alginate, which is applied in preparations for in vivo tests, pharmaceutical formulas, emulsion delivery, etc., can solve problems such as no discovery, and achieve uniform distribution, good water solubility, and simple operation

Inactive Publication Date: 2015-05-13
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Retrieval of domestic and foreign literature found that Fe 3 o 4 -Related reports on the preparation of sodium alginate nanogel as a MRI contrast agent with PEI as a cross-linking agent

Method used

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  • Preparation method of ferric oxide nanoparticle supported sodium alginate nanogel
  • Preparation method of ferric oxide nanoparticle supported sodium alginate nanogel
  • Preparation method of ferric oxide nanoparticle supported sodium alginate nanogel

Examples

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Embodiment 1

[0065] 1.25g FeCl 2 4H 2 Pour O into a beaker, add 7.75mL of ultrapure water, and add 6.25mL of NH 3 ·H 2 O, the above mixed solution was continuously stirred for 10 minutes under air atmosphere to fully oxidize the ferrous iron, and then the mixed solution was transferred to a high-pressure reactor. Dissolve 0.5g PEI in 5mL aqueous solution by ultrasonic, transfer it into the reaction kettle with a pipette gun, mix well with the solution in the reaction kettle, and react at 134°C for 3 hours. After the reaction was over, it was naturally cooled to room temperature, and the obtained black precipitate Fe 3 o 4 -PEI magnetic separation to remove the supernatant, add an appropriate amount of ultrapure water to ultrasonically disperse, and then magnetically separate, repeat the ultrapure water washing five times to remove impurities, and then redisperse in 20mL ultrapure water to obtain PEI coating Fe 3 o 4 Nanoparticles (Fe 3 o 4 -PEI). Take 3mL of 1wt% AG (30mg) aqueou...

Embodiment 2

[0067] Get the AG / PEI-Fe prepared in Example 1 3 o 4 Nanogel, which was diluted 100 times with ultrapure water, was used to measure surface potential and hydrodynamic diameter. Zeta potential measurement results show that AG / PEI-Fe 3 o 4 The surface potential of the nanogel is −7.58 mV, demonstrating that AG and Fe 3 o 4- Successful crosslinking of PEI. Its hydrodynamic diameter is 308.75±16.33nm, the particle size distribution is uniform, and the hydrodynamic diameter can remain almost unchanged for a long time, which shows that AG / PEI-Fe 3 o 4 Nanogel (Example 1) has good colloidal stability. Observation of AG / PEI-Fe prepared in embodiment 1 by transmission electron microscope (TEM) and atomic force microscope (AFM) afterwards 3 o 4 The morphology of nanogels (such as figure 1 ). The results show that the formed AG / PEI-Fe 3 o 4 The shape of the nanogel is spherical or quasi-spherical, the size is uniform, the diameter of the gel is about 170nm, there is no obvio...

Embodiment 3

[0069] Measure the AG / PEI-Fe that embodiment 1 prepares by ICP-AES testing method 3 o 4 The content of Fe element in the nanogel. Prepare AG / PEI-Fe with Fe concentration of 0.0025, 0.005, 0.01, 0.02, 0.04, 0.08mM respectively 3 o 4 Nanogel aqueous solution 2mL, the T of material under different Fe concentration is measured by magnetic resonance imaging analyzer 2 relaxation effects (such as figure 2 ). The relaxation rate test results show that AG / PEI-Fe 3 o 4 The reciprocal of the relaxation time of the nanogel has a good linear relationship with the increase of iron concentration (in the range of 0.0025-0.08mM concentration). AG / PEI-Fe is obtained by calculation 3 o 4 r of nanogel 2 The value is 170.81mM -1 the s -1 . Therefore, the AG / PEI-Fe prepared by the present invention 3 o 4 Nanogel can be used as an excellent T in MR molecular imaging diagnosis 2 Signal attenuating contrast agents.

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Abstract

The invention relates to a preparation method of ferric oxide nanoparticle supported sodium alginate nanogel. The preparation method comprises steps as follows: (1), PEI (polyethylenimine) coated Fe3O4 nano-particles (Fe3O4-PEI) are synthesized with a hydrothermal method; (2), an aqueous solution of sodium alginate is firstly activated by EDC (carbodiimide) and has a double emulsion reaction to form a W / O / W polymer emulsion; (3), Fe3O4-PEI in the step (1) is taken as a crosslinking agent and added into the polymer emulsion in the step (2) to have a crosslinking reaction, and the ferric oxide nanoparticle supported sodium alginate nanogel is obtained after an organic solvent and a surface active agent are removed. The method is very simple, and operation and separation are easy; meanwhile, sources of raw materials are extensive; the prepared sodium alginate nanogel has a smaller grain diameter, is uniformly distributed, high in relaxation rate and low in cost, has a remarkable radiography effect, simultaneously has excellent water solubility, gel stability, biocompatibility and blood compatibility, doesn't have a harmful effect on a living body, and has potential application value in the magnetic resonance imaging diagnosis field.

Description

technical field [0001] The invention belongs to the field of preparation of magnetic resonance imaging contrast agents, in particular to a preparation method of sodium alginate nanogel loaded with iron oxide nanoparticles. Background technique [0002] Nanogel is a three-dimensional network-like hydrogel particle composed of hydrophilic or amphiphilic polymer chains through physical or chemical cross-linking. It is a nanoscale soft material. Nanogels have many excellent properties, such as good colloidal stability, biocompatibility, high loading capacity, easy multifunctionalization, and easy entry into tumor tissues, etc., which promote their applications in many fields. Sodium alginate (Alginate, AG) is a natural polysaccharide, which has good biocompatibility and biodegradability, and is cheap and easy to obtain. It is widely used in the synthesis of nanogels. It is a by-product of extracting iodine and mannitol from brown algae kelp or sargassum, and is formed by linkin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K49/12
Inventor 史向阳孙文杰朱建志李静超周本青沈明武
Owner DONGHUA UNIV
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