The invention relates to a method for loading doxorubicine (DOX) anti-cancer medicine by laponite (LAP) clay nanoparticles. The method comprises the following steps that (1) LAP water solution is prepared and is mixed with DOX, then, the stirring is carried out, and mixed solution is obtained, wherein the concentration of the DOX in the DOX water solution is 2mg/mL, and the concentration of the LAP in the concentration water solution is 2 to 8mg/mL; and (2) the mixed solution is centrifugally separated, supernate is sucked out, in addition, ultra-pure water is used for washing precipitation, and finally, LAP loaded with DOX is obtained. The method has the advantages that the condition is mild, the process is simple, the product is easy to operate and separate, the LAP/DOX obtained by the method can effectively control the release of DOX, the LAP/DOX anti-tumor activity is obviously improved through being compared with that of the pure DOX, and the application prospects are wide.