HA-targeted layered doubled hydroxide-ultrafine iron nano material and preparation and applications thereof

A hydroxide and nanomaterial technology, which is applied to preparations for in vivo testing, medical preparations without active ingredients, and medical preparations containing active ingredients, etc., can solve the problem of not finding doxorubicin and ultra-small iron nanoparticles. Particle research reports, etc.

Active Publication Date: 2019-07-16
DONGHUA UNIV
View PDF8 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Searching the literature at home and abroad has not found any research reports on the preparation of layered doub

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • HA-targeted layered doubled hydroxide-ultrafine iron nano material and preparation and applications thereof
  • HA-targeted layered doubled hydroxide-ultrafine iron nano material and preparation and applications thereof
  • HA-targeted layered doubled hydroxide-ultrafine iron nano material and preparation and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0079] Example 1

[0080] Synthesis of Materials:

[0081] (1) under stirring FeCl 3 (1081 mg, Adamas reagent, 500 g, batch: P1276861) was dissolved in 40 mL of diethylene glycol to form a homogeneous solution. Sodium citrate (471 mg, Sinopharm, 500 g, batch number: 20140611) was added to the above solution, and the mixture was heated to 80°C in a water bath until a clear solution was formed. Subsequently, sodium acetate (1312 mg, Shanghai Lingfeng Chemical Reagent, 500 g, batch number: 20140124) was added to the above mixture solution and dissolved, and then the mixture was transferred to a Teflon-lined stainless steel autoclave with a volume of 100 mL and sealed in air . The autoclave was placed in an oven at 200 °C for 4 h. After cooling to room temperature, the black solution was collected by centrifugation (10000 rpm, 5 min) and purified three times with ethanol to remove excess reactants and by-products. The resulting black product was redispersed in water and lyoph...

Example Embodiment

[0085] Example 2

[0086] To evaluate LDH-Fe 3 O 4 -The imaging effect of HA as an MRI contrast agent, combining the material with pure Fe 3 O 4 nanoparticle r 1 The relaxation rates are compared, r 1 The relaxation rate is the longitudinal relaxation time of iron per unit molar concentration, which can be determined by different concentrations of T 1 The reciprocal fit of the relaxation time is calculated. Determination of Fe prepared in Example 1 by ICP-AES test method 3 O 4 , LDH-Fe 3 O 4 and LDH-Fe 3 O 4 - Content of Fe element in HA. Prepare LDH-Fe with Fe concentrations of 0.1, 0.2, 0.4, 0.8 and 1.6 mM, respectively 3 O 4 - 500 μL of aqueous solution of HA, the T of the material at different Fe concentrations was determined by magnetic resonance imaging analyzer 1 relaxation effects (such as Figure 8 ). Fe calculated 3 O 4 , LDH-Fe 3 O 4 and LDH-Fe 3 O 4 -r of HA 1 values ​​were 0.42, 5.53 and 4.38 mM, respectively -1 s -1 . LDH-Fe 3 O 4 -r ...

Example Embodiment

[0087] Example 3

[0088] Using B16 cells as a model to detect LDH-Fe by CCK-8 3 O 4 - Cytotoxicity of HA NPs and LDH-Fe 3 O 4 - In vitro anticancer effect of HA / DOX NPs. The concentration of iron prepared is 1000 μg / mL LDH-Fe 3 O 4 - PBS stock solution of HA NPs, then use sterile PBS to prepare DOX concentration and add 10 μL LDH-Fe of different concentrations on the ultra-clean bench 3 O 4- HA / DOX in PBS. The final DOX concentrations were 1.6, 3.2, 6.3, 12.5 and 25 μg / mL, and the carrier content corresponding to the highest concentration was made into one group. And sterilized by UV irradiation overnight. Continue to place the cell culture plate in 5% CO 2 , 37 ℃ continue to incubate for 24h and 48h. Then pour off the culture medium, wash twice with PBS, add 100 μL of new culture medium (containing 10 μL CCK-8, 90 μL medium) to each well, continue to culture for 4 h, measure the absorbance at 450 nm with a microplate reader, and The viability of the cells was cal...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention relates to a HA-targeted layered doubled hydroxide-ultrafine iron nano material and preparation and applications thereof. The preparation is as follows: synthesizing an LDH-Fe3O4 nano composite material by a coprecipitation method; covalently bonding activated hyaluronic acid on the surface of an LDH laminate through a silane coupling agent; and finally, carrying out surface loadingof an anticancer drug. The nano-material particles are uniformly distributed, and can enhance the MR imaging effect of the tumor part in an animal body. The LDH-Fe3O4-HA NPs is used as a carrier of ananticancer drug doxorubicin DOX, not only has sensitive pH response and release characteristics, but also can carry out specific recognition on tumor cells expressed by CD44 receptors so as to achieve the idealization effect of efficiently inhibiting tumors.

Description

technical field [0001] The invention belongs to the field of nano-diagnosis and treatment agent and its preparation and application, in particular to an HA-targeted double metal hydroxide-ultrasmall iron nanometer material and its preparation and application. Background technique [0002] In recent years, with the development of nanotechnology and molecular imaging, the construction of a nanodiagnosis and treatment platform with tumor diagnosis and treatment functions provides the possibility for early diagnosis and individualized treatment of tumors. MR imaging is regarded as one of the most effective clinical diagnostic techniques for early cancer due to its advantages of high sensitivity, high resolution, and no trauma or radiation effects. Compared with the commonly used MR positive contrast agents containing Gd and Mn, the ultra-small size of ferric oxide nanoparticles (<5nm) not only has good T 1 Imaging effect, also has better biocompatibility, is a potential high...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K49/18A61K49/12A61K9/51A61K47/36A61K31/704A61P35/00
CPCA61K9/5115A61K9/5161A61K31/704A61K49/126A61K49/1863A61P35/00
Inventor 郭睿张妮史向阳
Owner DONGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap