The invention relates to a method for determining the conversion rate of polyisobutylene, in particular to a method for determining the conversion rate of the polyisobutylene during a process of generating p-polyisobutylene phenol through reaction of the polyisobutylene and excessive phenol through a quantitative nuclear magnetic resonance spectroscopy. The method comprises the steps of respectively acquiring a polyisobutylene phenol sample, and a polyisobutylene and phenol nuclear magnetic resonance spectroscopy, obtaining chemical shift of the p-polyisobutylene phenol through spectrogram comparison and spectrogram reasoning; determining longitudinal relaxation time T1 of a p-polyisobutylene phenol sample in a deuteration reagent, setting a pulse flip angle and relaxation time of a nuclear magnetic resonance spectrometer according to the T1, utilizing the nuclear magnetic resonance spectrometer for determining the nuclear magnetic resonance spectroscopy of a solution to be tested, obtaining a peak area of each part of hydrogen through integration, and calculating to obtain the conversion rate of the polyisobutylene through the number of hydrogen actually contained in a target product and raw materials. The method provided by the invention is low in cost, can be used for quickly and accurately evaluating the type and the conversion rate of a synthetic product of the polyisobutylene and the phenol, and provides a powerful evidence for synthesis.