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pH value testing method

A determination method, amino proton technology, applied in the field of biomedicine, can solve the problems of difficulty, the premise cannot be strictly proved, and the high chemical exchange rate of amine groups, so as to eliminate the influence of concentration, eliminate the dependence of longitudinal relaxation time, and have a good application prospect Effect

Active Publication Date: 2018-06-22
SHENZHEN INST OF ADVANCED TECH
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Problems solved by technology

However, this method assumes that the amine group and the amino group originate from the same macromolecule, which cannot be strictly proved; in addition, the chemical exchange rate of the amine group is generally high, and it is difficult to capture its CEST effect under the magnetic resonance field strength commonly used in clinical practice. Application is quite difficult

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Embodiment 1

[0060] A method for measuring pH, comprising the steps of:

[0061] (a) making several phantoms after loading polylysine solutions with different pHs, the pHs are 5.5, 6.0, 6.5, 7.0, 7.5 and 8.0;

[0062] (b) performing a chemical exchange saturation transfer imaging experiment on the phantom under different saturation pulse intensities;

[0063] Assuming that the chemical exchange rates of solutions with pH 5.5, 6.0, 6.5, 7.0, 7.5 and 8.0 are 10, 30, 60, 90, 120 and 180 Hz, respectively, the concentration of amino protons relative to water is 1:200, and the longitudinal and transverse directions of water are set The relaxation time is 1.2s and 80ms respectively, the continuous radio frequency pulse saturation time is 2.4s, the frequency offset range is -6~6ppm, and the step size is 0.1ppm; under the 3T magnetic resonance field strength, the saturation pulse intensity is 0.75μT and 1.5μT, the Z spectrum as figure 1 shown;

[0064] (c) Calculate the chemical exchange saturat...

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Abstract

The invention relates to the field of biological medicines, in particular to a pH value testing method which comprises the following steps: with an amino proton as an endogenous contrast agent, testing corresponding chemical exchange saturated transferring effect ratios R of amino protons of known pH value amino proton source solutions under different saturated pulse intensities, establishing function relationships of pH values and the ratios R corresponding to the different known pH value amino proton source solutions according to the different known pH value amino proton source solutions andtested corresponding ratios R, and finally calculating expected pH values according to the function relationships of values Ri tested through experiments and the function relationships. By adopting the method, influence of concentrations can be eliminated, parameters such as exchangeable proton concentrations and longitudinal relaxation times of water do not need to be estimated or measured, so that pH values can be relatively accurately, conveniently and non-destructively measured. In addition, since amino is relatively low in chemical exchange velocity, stable imaging under magnetic resonance field intensity of common clinical application can be achieved, and thus the method has good application prospects.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for measuring pH. Background technique [0002] Intracellular pH is an important parameter of the acid-base environment of biological tissues, and is closely related to the metabolic state of the body, which is helpful for understanding the mechanism, development process and prognosis evaluation of diseases. [0003] Chemical Exchange Saturation Transfer (CEST) imaging is a magnetic resonance molecular imaging method, based on the chemical exchange between the exchangeable protons and water molecules in endogenous or exogenous contrast agents, it can realize the chemical exchange rate. Determination. Some contrast agents (such as amino protons) are sensitive to pH environment, and their chemical exchange rate is closely related to pH, thus providing a possible way for pH measurement. [0004] The existing methods for measuring intracellular pH first all need to measure the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08
CPCG01N24/08G01R33/5605G01R33/5608G01R33/5601
Inventor 吴垠郑海荣刘新
Owner SHENZHEN INST OF ADVANCED TECH
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