The invention provides a milk
flavor essence and a preparation method thereof. The method comprises the following steps: preparing an activated bacterium solution from German-type
lactobacillus bulgaricus
subspecies;
anhydrous cream and whey
powder are prepared into a
fermentation substrate; adding the activated bacterial liquid into the
fermentation substrate, and performing sealed
fermentation to obtain a fermentation product; and carrying out first sterilization treatment on the fermentation product, carrying out
centrifugal separation, and taking supernate as the milk-flavored essence. Whey
powder is added when a fermentation substrate is prepared, the whey
powder is rich in
whey protein,
lactose, vitamins and mineral substances, and glycometabolism,
protein metabolism and
lipid metabolism pathways are activated in the fermentation process, so that a fermentation product not only can be rich in target milk
flavor substances, but also can synergistically contain various
flavor compounds such as esters, alcohols and aldehydes, and the richness of the flavor substances is improved; the
anhydrous cream and the whey powder are prepared into the fermentation substrate, so that a comprehensive and balanced growth environment is provided for the German-type
lactobacillus bulgaricus
subspecies, and the fermentation efficiency is improved.