11 results about "Tobacco leaf extract" patented technology

The invention discloses an Apiotrichum loubieri gene and an application of the Apiotrichum loubieri gene. Relates to the technical field of fermentation. The invention provides a specific strain and application thereof in reconstituted tobacco. According to the tobacco leaf extracting solution fermented by the bacterial strain, the content of esters such as 2, 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate, dimethyl phthalate, tributyl phosphate, dihydroactinidiolide and methyl palmitate is remarkably increased, and the content of the esters such as 2, 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate, dimethyl phthalate, tributyl phosphate, dihydroactinidiolide and methyl palmitate is remarkably increased; nine novel ester substances with aromatic smell, fruit fragrance, flower fragrance and other characteristic aromas are newly generated, and four phenolic aroma components are newly added. And the whole fragrance style is obviously enhanced. It is shown that the aroma-producing Apiotrichum loubieri AL-4 fermented tobacco enzyme extracting solution has good aroma-producing capacity, and through the combined effect of enzymolysis and fermentation, the aroma-producing Apiotrichum loubieri AL-4 fermented tobacco enzyme extracting solution is expected to play an important application value in the field of reconstituted tobacco raw material quality improvement.

The invention relates to a determination method and application of a Henan tobacco leaf sensory quality key group, and belongs to the field of preparation of tobacco leaf sensory quality key groups. The preparation method comprises the following steps: (1) carrying out gel column chromatography separation on Henan tobacco leaf extract, and segmenting fractions according to ultraviolet absorption conditions to obtain separated components; (2) carrying out independent evaluation and deletion recombination evaluation on each separated component; and (3) determining the sensory quality key group highlighting the sweet aroma style characteristic according to the evaluation result in the step (2). By means of the method, the group playing a positive role in the cigarette smoking quality can be obtained, and theoretical guidance is provided for tobacco leaf quality evaluation, tobacco flavor blending and the like.

The application belongs to the technical field of tobacco, and particularly relates to a method for real-time adjustment of flow of premixed tobacco leaf extract or tobacco stem extract. The method provided in the application can adjust the flow of the tobacco stem extract and the tobacco leaf extract in real time through real-time detection of the solid content, so as to obtain coating liquid with stable quality, and thus the sensory quality of tobacco smoking is improved.

The application discloses a microbial preparation for straw decomposition and soil nutrient improvement and application thereof, and relates to the technical field of agricultural waste resource utilization and soil improvement. Streptomyces sp. PLXSD-1, and the microbial preparation is prepared by compounding the streptomycete and a tobacco leaf extract, and the microbial preparation can promote straw degradation and improve soil nutrient conditions. The activity of the streptomycete is improved by using the tobacco leaf extract, and the rapid decomposition of the straw and the supply capacity of soil nitrogen and phosphorus are improved. Specifically, compared with the streptomycete alone, the phosphorus solubilizing capacity is increased by 19.43% and the straw degradation rate is significantly increased by 25.82% after the streptomycete is compounded with the tobacco leaf extract. In addition, the streptomycete PLXSD-1 and the tobacco leaf extract have a synergistic effect on improving the straw degradation rate.

The present application relates to a kind of preparation methods of waste tobacco extract, waste tobacco is crushed, enzymolysis, organic solvent ultrasonic extraction obtains methylene chloride extract S1 and ethyl acetate extract S2;After enzymolysis treatment, tobacco powder is fermented, subcritical water extraction, heavy metal adsorption and nanofiltration membrane filtration, nanofiltration membrane permeate is concentrated to obtain concentrated extract, concentrated extract is carried out molecular distillation, the light component and heavy component of molecular distillation are collected, the light component is water extract S3, and the heavy component is water extract S4;Concentrated extract is obtained by concentrating the cut-off liquid, and is recorded as water extract S5;The waste tobacco extract is one or more than two of methylene chloride extract S1, ethyl acetate extract S2, water extract S3, water extract S4 and water extract S5.The extract contains a large amount of tobacco native substance, has strong tobacco style characteristics, and higher safety, and it is applied to cigarette, can significantly improve the sensory quality of cigarette.

The invention discloses a tobacco leaf extract degrading bacterium YY-2-4-1, a screening method and application thereof, and a method for treating tobacco leaf extraction wastewater by using the tobacco leaf extract degrading bacterium YY-2-4-1. The tobacco leaf extract degrading bacterium YY-2-4-1 is acidophilic oligodeoxymonas and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.36759, the preservation number is CGMCC NO.36759, and the preservation number is CGMCC NO.36759. The pure strain YY2-4-1 is obtained from an original strain obtained from a waste water treatment plant of tobacco leaf extraction waste water by taking a tobacco leaf extract as a raw material through the steps of acclimatization, separation, purification and the like of a culture medium containing a single pollutant of the tobacco leaf extract, and the degradation efficiency of the pure strain YY2-4-1 on the tobacco leaf extraction waste water is 76.6%; pollutants in the tobacco extraction wastewater can be stably and efficiently removed, and the wastewater treatment cost is reduced.

This tobacco composition production method includes a feature of irradiating a raw material liquid containing a photosynthetic pigment, the raw material liquid selected from a tobacco leaf extract and a tobacco leaf dispersion, with irradiation light selected from red light and blue light, to obtain a tobacco composition having a less nicotine content compared to the raw material liquid.

The invention discloses a microbial preparation for straw decomposition and soil nutrient improvement and application thereof, and relates to the technical field of agricultural waste recycling and soil improvement. The streptomyces sp. PLXSD-1 capable of dissolving phosphorus, fixing nitrogen and promoting straw decomposition is separated from straw returning soil, the streptomyces and a tobacco leaf extract are compounded to prepare the microbial preparation, and the microbial preparation can promote straw degradation and improve the soil nutrient condition. The tobacco leaf extract is used for improving the activity of streptomycete, promoting rapid decomposition of straw and improving the nitrogen and phosphorus supply capacity of soil. Specifically, compared with single streptomycete, after the streptomycete is compounded with a tobacco leaf extract, the phosphate solubilizing capacity is improved by 19.43%, and the straw degradation rate is remarkably increased by 25.82%. In addition, the streptomyces PLXSD-1 and the tobacco leaf extract are combined to achieve a synergistic effect on improvement of the straw degradation rate.

The application provides a composition with a killing effect on golden apple snails and application thereof, and belongs to the technical field of pest control. Effective active ingredients of the composition include discarded tobacco leaf extract and orange peel essential oil. The composition is extracted from natural plants, has high efficiency and safety, can effectively achieve the prevention and control of harmful soft-bodied animals, i.e., golden apple snails, has a significant killing effect on the golden apple snails at a low concentration, and is verified by experiments to have no toxicity to 3-4 cm carp fry at an effective concentration, fully indicating that the composition is safe to non-target organisms and has low environmental risk.

The invention belongs to the technical field of cigarette flavors and fragrances, and discloses a preparation method and application of 2-phenethyl alcohol fermentation liquor. The method comprises the following steps: mixing a tobacco material and water according to a solid-liquid ratio of 1: (5-12) (g / mL), extracting at 90-115 DEG C for 20-40 minutes, and carrying out solid-liquid separation to obtain a tobacco leaf extract culture medium; carrying out degerming treatment on the culture medium, and then adding L-phenylalanine to obtain a preference phenylalanine culture medium; and inoculating saccharomycetes capable of synthesizing 2-phenethyl alcohol, and carrying out fermentation culture to obtain 2-phenethyl alcohol fermentation liquor. The invention not only provides a feasible technical scheme for high-efficiency and low-cost biosynthesis of 2-phenethyl alcohol, but also opens up a new way for high-valued utilization of tobacco byproducts and high-efficiency biosynthesis of natural perfume, and has a wide application prospect.

The invention relates to a screening method of tobacco leaf quality comprehensive evaluation indexes and a construction method of an evaluation model, and belongs to the field of establishment of tobacco leaf quality comprehensive evaluation indexes and evaluation models. The method comprises the following steps: obtaining separated components of a tobacco leaf extract, then carrying out independent evaluation and deletion recombination evaluation on each separated component, determining a sensory quality key group highlighting sensory characteristics of tobacco leaves, and then carrying out qualitative and quantitative analysis on chemical compositions of the sensory quality key groups corresponding to various tobacco leaves in the same region, calculating to obtain relative indexes of each chemical composition, and determining a comprehensive evaluation index of the tobacco quality through Pearson correlation analysis. The comprehensive evaluation index gives consideration to factors influencing quality characteristics and style characteristics of tobacco products, and the accuracy of tobacco quality judgment is improved.