19 results about "Tobacco leaf extract" patented technology

The invention discloses mongolian mongolian yeast and an application thereof. Relates to the technical field of fermentation. The invention provides a specific strain and application thereof in tobacco leaves. According to the tobacco leaf extracting solution fermented by the bacterial strain, the content of esters such as 2, 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate, tributyl phosphate, dihydroactinidiolide, dibutyl phthalate, 3-(3, 5-di-tert-butyl-4-hydroxyphenyl) methyl propionate and methyl palmitate is remarkably increased, and the content of the esters such as 2, 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate, tributyl phosphate, dihydroactinidiolide, dibutyl phthalate, 3-(3, 5-di-tert-butyl-4-hydroxyphenyl) methyl propionate and methyl palmitate is remarkably increased; three novel ester substances with fruit fragrance, rosin and other characteristic fragrances are newly generated, and the overall fragrance style is remarkably enhanced. It is shown that the aroma-producing caribeckia mongolica yeast MC-5 fermented tobacco leaf extracting solution has good aroma-producing capacity, and through the combined effect of enzymolysis and fermentation, the aroma-producing caribeckia mongolica yeast MC-5 fermented tobacco leaf extracting solution is expected to play an important application value in the field of quality improvement and aroma enhancement of tobacco leaf raw materials.

The invention discloses issatchenkia orientalis and application thereof. Relates to the technical field of fermentation. The invention provides a specific strain and application thereof in reconstituted tobacco. According to the tobacco leaf extracting solution fermented by the bacterial strain, the content of esters such as 2, 2, 4-trimethylpentanediol isobutyl ester, 2, 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate, tributyl phosphate and methyl palmitate is remarkably increased, eight novel ester substances with the characteristic aroma such as fruit aroma and rosin are newly generated, and the tobacco leaf extracting solution has the advantages that the content of the esters such as the 2, 2, 4-trimethylpentanediol isobutyl ester, the 2, 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate, the tributyl phosphate and the methyl palmitate is increased; and the whole fragrance style is obviously enhanced. It is shown that the aroma-producing issatchenkia orientalis IO-1 fermented tobacco leaf extracting solution has good aroma-producing capacity, and through the combined effect of enzymolysis and fermentation, the extracting solution is expected to play an important application value in the field of reconstituted tobacco leaf raw material quality improvement.

The invention discloses an Apiotrichum loubieri gene and an application of the Apiotrichum loubieri gene. Relates to the technical field of fermentation. The invention provides a specific strain and application thereof in reconstituted tobacco. According to the tobacco leaf extracting solution fermented by the bacterial strain, the content of esters such as 2, 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate, dimethyl phthalate, tributyl phosphate, dihydroactinidiolide and methyl palmitate is remarkably increased, and the content of the esters such as 2, 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate, dimethyl phthalate, tributyl phosphate, dihydroactinidiolide and methyl palmitate is remarkably increased; nine novel ester substances with aromatic smell, fruit fragrance, flower fragrance and other characteristic aromas are newly generated, and four phenolic aroma components are newly added. And the whole fragrance style is obviously enhanced. It is shown that the aroma-producing Apiotrichum loubieri AL-4 fermented tobacco enzyme extracting solution has good aroma-producing capacity, and through the combined effect of enzymolysis and fermentation, the aroma-producing Apiotrichum loubieri AL-4 fermented tobacco enzyme extracting solution is expected to play an important application value in the field of reconstituted tobacco raw material quality improvement.

The invention relates to a determination method and application of a Henan tobacco leaf sensory quality key group, and belongs to the field of preparation of tobacco leaf sensory quality key groups. The preparation method comprises the following steps: (1) carrying out gel column chromatography separation on Henan tobacco leaf extract, and segmenting fractions according to ultraviolet absorption conditions to obtain separated components; (2) carrying out independent evaluation and deletion recombination evaluation on each separated component; and (3) determining the sensory quality key group highlighting the sweet aroma style characteristic according to the evaluation result in the step (2). By means of the method, the group playing a positive role in the cigarette smoking quality can be obtained, and theoretical guidance is provided for tobacco leaf quality evaluation, tobacco flavor blending and the like.

The application belongs to the technical field of tobacco, and particularly relates to a method for real-time adjustment of flow of premixed tobacco leaf extract or tobacco stem extract. The method provided in the application can adjust the flow of the tobacco stem extract and the tobacco leaf extract in real time through real-time detection of the solid content, so as to obtain coating liquid with stable quality, and thus the sensory quality of tobacco smoking is improved.

The invention relates to a botanical insecticidal composition containing nicotine and veratrine extract and application of the botanical insecticidal composition. The botanical insecticidal composition comprises tobacco leaf extract nicotine and veratrine in a mass ratio of (4-7): (3-6). According to the botanical insecticidal composition provided by the invention, through a large number of experimental research screening, the tobacco leaf extracts nicotine and veratrine are mixed according to a specific mass ratio to achieve a synergistic effect, under the same dosage, the botanical insecticidal composition has more excellent insecticidal and acaricidal effects, the use concentration of the compound insecticidal composition can be greatly reduced, the cost is lower, and the effect is better. And the environmental pollution is less. The botanical insecticidal composition provided by the invention has a good control effect on tetranychus urticae of rosa chinensis and solanaceae crops, and has important economic value and environmental protection value.

The application discloses a microbial preparation for straw decomposition and soil nutrient improvement and application thereof, and relates to the technical field of agricultural waste resource utilization and soil improvement. Streptomyces sp. PLXSD-1, and the microbial preparation is prepared by compounding the streptomycete and a tobacco leaf extract, and the microbial preparation can promote straw degradation and improve soil nutrient conditions. The activity of the streptomycete is improved by using the tobacco leaf extract, and the rapid decomposition of the straw and the supply capacity of soil nitrogen and phosphorus are improved. Specifically, compared with the streptomycete alone, the phosphorus solubilizing capacity is increased by 19.43% and the straw degradation rate is significantly increased by 25.82% after the streptomycete is compounded with the tobacco leaf extract. In addition, the streptomycete PLXSD-1 and the tobacco leaf extract have a synergistic effect on improving the straw degradation rate.

The present application relates to a kind of preparation methods of waste tobacco extract, waste tobacco is crushed, enzymolysis, organic solvent ultrasonic extraction obtains methylene chloride extract S1 and ethyl acetate extract S2;After enzymolysis treatment, tobacco powder is fermented, subcritical water extraction, heavy metal adsorption and nanofiltration membrane filtration, nanofiltration membrane permeate is concentrated to obtain concentrated extract, concentrated extract is carried out molecular distillation, the light component and heavy component of molecular distillation are collected, the light component is water extract S3, and the heavy component is water extract S4;Concentrated extract is obtained by concentrating the cut-off liquid, and is recorded as water extract S5;The waste tobacco extract is one or more than two of methylene chloride extract S1, ethyl acetate extract S2, water extract S3, water extract S4 and water extract S5.The extract contains a large amount of tobacco native substance, has strong tobacco style characteristics, and higher safety, and it is applied to cigarette, can significantly improve the sensory quality of cigarette.

The invention discloses a tobacco leaf extract degrading bacterium YY-2-4-1, a screening method and application thereof, and a method for treating tobacco leaf extraction wastewater by using the tobacco leaf extract degrading bacterium YY-2-4-1. The tobacco leaf extract degrading bacterium YY-2-4-1 is acidophilic oligodeoxymonas and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.36759, the preservation number is CGMCC NO.36759, and the preservation number is CGMCC NO.36759. The pure strain YY2-4-1 is obtained from an original strain obtained from a waste water treatment plant of tobacco leaf extraction waste water by taking a tobacco leaf extract as a raw material through the steps of acclimatization, separation, purification and the like of a culture medium containing a single pollutant of the tobacco leaf extract, and the degradation efficiency of the pure strain YY2-4-1 on the tobacco leaf extraction waste water is 76.6%; pollutants in the tobacco extraction wastewater can be stably and efficiently removed, and the wastewater treatment cost is reduced.

The application discloses a preparation method of tobacco leaf extract. The effective components in the tobacco leaf are extracted to a high degree through repeated or continuous rolling and extraction of tobacco leaf fragments, and the tobacco leaf extract is prepared with high efficiency. The application also uses an optimal device to improve the overall efficiency of the device and method, including a cylindrical rotary rolling part, a rolling plate capable of adjusting rolling conditions, a large-area rolling surface and filter holes integrated with the bin wall, and a temperature control part capable of providing temperature control function for most of the device.

This tobacco composition production method includes a feature of irradiating a raw material liquid containing a photosynthetic pigment, the raw material liquid selected from a tobacco leaf extract and a tobacco leaf dispersion, with irradiation light selected from red light and blue light, to obtain a tobacco composition having a less nicotine content compared to the raw material liquid.

The invention discloses a microbial preparation for straw decomposition and soil nutrient improvement and application thereof, and relates to the technical field of agricultural waste recycling and soil improvement. The streptomyces sp. PLXSD-1 capable of dissolving phosphorus, fixing nitrogen and promoting straw decomposition is separated from straw returning soil, the streptomyces and a tobacco leaf extract are compounded to prepare the microbial preparation, and the microbial preparation can promote straw degradation and improve the soil nutrient condition. The tobacco leaf extract is used for improving the activity of streptomycete, promoting rapid decomposition of straw and improving the nitrogen and phosphorus supply capacity of soil. Specifically, compared with single streptomycete, after the streptomycete is compounded with a tobacco leaf extract, the phosphate solubilizing capacity is improved by 19.43%, and the straw degradation rate is remarkably increased by 25.82%. In addition, the streptomyces PLXSD-1 and the tobacco leaf extract are combined to achieve a synergistic effect on improvement of the straw degradation rate.

The application provides a composition with a killing effect on golden apple snails and application thereof, and belongs to the technical field of pest control. Effective active ingredients of the composition include discarded tobacco leaf extract and orange peel essential oil. The composition is extracted from natural plants, has high efficiency and safety, can effectively achieve the prevention and control of harmful soft-bodied animals, i.e., golden apple snails, has a significant killing effect on the golden apple snails at a low concentration, and is verified by experiments to have no toxicity to 3-4 cm carp fry at an effective concentration, fully indicating that the composition is safe to non-target organisms and has low environmental risk.

The invention provides a raw material pretreatment method for improving the efficacy of a tobacco leaf extract and application of the raw material pretreatment method. The method comprises the steps that tobacco leaves are subjected to the procedures of loosening and moisture regaining, feeding and leaf storing in sequence, and tobacco shreds are obtained after shredding; the tobacco shreds are sequentially subjected to heating and humidifying, tobacco shred drying, winnowing and tobacco shred storage procedures, and a pretreatment raw material for preparing the tobacco leaf extract is obtained; the step of adding materials comprises adding auxiliary materials and water; the auxiliary materials comprise sugar and / or acid; the sugar comprises any one or a combination of at least two of glucose, fructose, bee sugar, invert sugar or high fructose corn syrup; the acid comprises any one or a combination of at least two of malic acid, lactic acid, acetic acid or citric acid. According to the raw material pretreatment method, the comfort of the tobacco leaf extract can be improved, and the tobacco characteristic fragrance can be enhanced. The charging and cut tobacco drying processes can synergistically reduce the nicotine content in the tobacco leaf extract and increase the aroma component content.

The invention belongs to the technical field of cigarette flavors and fragrances, and discloses a preparation method and application of 2-phenethyl alcohol fermentation liquor. The method comprises the following steps: mixing a tobacco material and water according to a solid-liquid ratio of 1: (5-12) (g / mL), extracting at 90-115 DEG C for 20-40 minutes, and carrying out solid-liquid separation to obtain a tobacco leaf extract culture medium; carrying out degerming treatment on the culture medium, and then adding L-phenylalanine to obtain a preference phenylalanine culture medium; and inoculating saccharomycetes capable of synthesizing 2-phenethyl alcohol, and carrying out fermentation culture to obtain 2-phenethyl alcohol fermentation liquor. The invention not only provides a feasible technical scheme for high-efficiency and low-cost biosynthesis of 2-phenethyl alcohol, but also opens up a new way for high-valued utilization of tobacco byproducts and high-efficiency biosynthesis of natural perfume, and has a wide application prospect.

This invention relates to an e-liquid with a refreshing snail flavor and its preparation method, belonging to the field of e-liquid technology. The e-liquid prepared by this invention has a refreshing and long-lasting flavor, a delicate and ethereal aroma, is mild and non-irritating, and has a rich taste that leaves a lasting impression. The e-liquid of this invention comprises a refreshing extract, tobacco flavoring, tobacco leaf extract, snail flavoring, medicinal flavoring agent, agar, glycerin, and olive oil. This e-liquid uses agar and olive oil as flavor-locking agents, which not only preserves the snail flavor for a long time but also provides a subtle, refreshing plant sensation upon closer tasting, giving a sense of tranquility and relaxation, like being in nature. The addition of the medicinal flavoring agent, when mixed with the e-liquid, can relieve dizziness, headaches, and reduce swelling caused by prolonged sitting or colds. This not only preserves the flavor of e-cigarettes but also enriches the flavor options currently available on the market and reduces the health risks associated with e-cigarettes.

The present disclosure generally relates to a treatment method for helping a subject quit smoking, the method comprising at least one step of administering to the subject an effective dose of an aqueous solution of protein-enriched tobacco leaf extract while the subject continues to smoke freely.Optionally, the second step comprises providing the subject with at least one product capable of controlling nicotine withdrawal symptoms, such as a nicotine agonist or partial agonist, and / or a nicotine substitute, by one or several optional complementary administrations.The present disclosure also relates to a kit comprising a protein-enriched tobacco leaf extract and at least one product capable of controlling nicotine withdrawal symptoms, such as a nicotine agonist or partial agonist, and / or a nicotine substitute.

The invention discloses a method for preparing a nematicide from cigar fermented tobacco leaf extract and the nematicide. The method comprises the following steps: crushing dried cigar fermented tobacco leaves by using a crusher to obtain a coarse crushed material with the particle size of 1-5mm; performing cold soaking on the coarsely crushed material for 24-48 hours through an organic solvent, and repeating for 3-5 times to obtain cigar fermented tobacco leaf extract liquid; and repeatedly extracting the cigar fermented tobacco leaf extract liquid through an extraction liquid, pressurizing and concentrating the cigar fermented tobacco leaf extract liquid to 1 / 9-1 / 11 of the mass of the dried cigar fermented tobacco leaf through a rotary evaporator to obtain a cigar fermented tobacco leaf extract, adding an organic solvent, an anti-freezing agent and sterile water, and fully stirring to prepare the nematicide. The cigar fermented tobacco leaf nematicide with remarkable nematicidal activity is prepared, the nematicidal activity is remarkable, no chemical residue exists, the environment compatibility is good, the cost is reduced, and the problems that a plant source nematicide is weak in activity and high in cost are solved.

The invention relates to a screening method of tobacco leaf quality comprehensive evaluation indexes and a construction method of an evaluation model, and belongs to the field of establishment of tobacco leaf quality comprehensive evaluation indexes and evaluation models. The method comprises the following steps: obtaining separated components of a tobacco leaf extract, then carrying out independent evaluation and deletion recombination evaluation on each separated component, determining a sensory quality key group highlighting sensory characteristics of tobacco leaves, and then carrying out qualitative and quantitative analysis on chemical compositions of the sensory quality key groups corresponding to various tobacco leaves in the same region, calculating to obtain relative indexes of each chemical composition, and determining a comprehensive evaluation index of the tobacco quality through Pearson correlation analysis. The comprehensive evaluation index gives consideration to factors influencing quality characteristics and style characteristics of tobacco products, and the accuracy of tobacco quality judgment is improved.