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Preparation method for irritation reducing fermentation tobacco extract, and applications of irritation reducing fermentation tobacco extract in recombinant tobacco

A technology of tobacco leaf extract and tobacco leaf, which is applied in the field of recombined tobacco leaf additives, can solve the problems of high irritation, heavy miscellaneous gas, and uncomfortable taste, and achieve the effect of improving large irritation, full smoke, and reducing smoke irritation

Inactive Publication Date: 2013-01-02
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the taste-absorbing defects such as heavy miscellaneous gas, high irritation and uncomfortable taste in the current recombined tobacco leaves, the present invention provides a preparation method of fermented tobacco leaf extract with reduced irritation and its application in recombined tobacco leaves

Method used

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  • Preparation method for irritation reducing fermentation tobacco extract, and applications of irritation reducing fermentation tobacco extract in recombinant tobacco
  • Preparation method for irritation reducing fermentation tobacco extract, and applications of irritation reducing fermentation tobacco extract in recombinant tobacco

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Effect test

Embodiment 1

[0025] The preparation method of the microbial plant Raoultella planticola VP4-4 CCTCC M 2012005 biotransformation production fermented tobacco leaf extract of the present invention is as follows:

[0026] 1) Crush the tobacco leaves to 100 mesh, then reflux extract the tobacco powder and water at a mass ratio of 1:60 for 3 hours, then filter, repeat the reflux once to extract the filtrate, and combine the filtrates to obtain the tobacco leaf extract, which is then sterilized;

[0027] 2) Strain activation: inoculate Raoultella planticola VP4-4 CCTCC M 2012005 on the slant medium, and culture at 38°C for 24 hours to obtain the activated strain, and place it in a refrigerator at 4°C;

[0028] The slant culture medium is: wort juice: 120mL, sucrose: 20g / L, agar: 15g / L.

[0029] 3) Preparation of seed culture solution: inoculate the activated strains obtained in step 2) into the seed culture medium, culture at 25°C with shaking at 100r / min for 72 hours, and transfer continuously ...

Embodiment 2

[0034] The preparation method of the microbial Raoultella planticola VP4-4 CCTCC M 2012005 biotransformation production fermented tobacco leaf extract of the present invention is as follows:

[0035] 1) Crush the tobacco leaves to 100 mesh, then reflux extract the tobacco powder and water at a mass ratio of 1:40 for 3 hours, then filter, repeat the reflux extraction filtrate 3 times, and combine the filtrates to obtain the tobacco leaf extract, and sterilize it;

[0036] 2) Activation of strains: Inoculate Raoultella planticola VP4-4 CCTCC M 2012005 on the slant medium, culture at 30°C for 72 hours to obtain activated strains, and place them in a refrigerator at 4°C;

[0037] The slant culture medium is: wort juice: 120mL, sucrose: 20g / L, agar: 15g / L.

[0038]3) Preparation of seed culture solution: Inoculate the activated strains obtained in step 2) into the seed culture medium, culture at 32°C with shaking at 100r / min for 48 hours, and transfer continuously for 3 times to ob...

Embodiment 3

[0043] The preparation method of the microbial Raoultella planticola VP4-4 CCTCC M 2012005 biotransformation production fermented tobacco leaf extract of the present invention is as follows:

[0044] 1) Crush the tobacco leaves to 50 mesh, then reflux extract the tobacco powder and water at a mass ratio of 1:20 for 1 hour, then filter, repeat the reflux once to extract the filtrate, and combine the filtrates to obtain the tobacco leaf extract, which is then sterilized;

[0045] 2) Strain activation: inoculate Raoultella planticola VP4-4 CCTCC M 2012005 on the slant medium, and culture at 25°C for 24 hours to obtain the activated strain, and place it in the refrigerator;

[0046] The slant culture medium is: wort juice: 120mL, sucrose: 20g / L, agar: 15g / L.

[0047] 3) Preparation of seed culture solution: Inoculate the activated strain obtained in step 2) into the seed medium, shake and culture at 100r / min for 72 hours at 25°C, and transfer once continuously to obtain the seed c...

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Abstract

The present invention discloses a method for an irritation reducing fermentation tobacco extract, and applications of the irritation reducing fermentation tobacco extract in recombinant tobacco. According to the present invention, a name of strain is Raoultella planticola VP4-4, a preservation number is CCTCC M 2012005, the total 16SrRNA sequence length is 1408 bp, and similarity between the 16SrRNA sequence and Raoultella planticola is 99.563%. According to the method, the Raoultella planticola VP4-4 is adopted, and bacteria seed activation, seed culture liquid preparation, fermentation culture and fermentation solution transformation are performed to obtain the irritation reducing fermentation tobacco extract. With the present invention, the tobacco extract is adopted as a single carbon source, a nitrogen source, and a substrate, is a cigarette origin material, and is easily utilized by a microbe enzyme system to ferment so as to produce cigarette fragrance. In addition, the method of the present invention has a simple process, the raw material is the cigarette product origin tobacco, and the method has an industrial application prospect.

Description

technical field [0001] The invention relates to the technical field of recombined tobacco leaf additives, in particular to a method for preparing fermented tobacco leaf extract with reduced irritation and its application in recombined tobacco leaves. Background technique [0002] Reconstituted tobacco leaves, also known as reconstituted tobacco leaves or homogeneous tobacco leaves, are mainly composed of tobacco powder, chips, tobacco stems or low-grade tobacco leaves with adhesives and other additives. The existing domestic papermaking thin sheet production process extracts the water-soluble substances in tobacco leaves and tobacco stems with water, and after solid-liquid separation, the solid part is pulped and then made into a paper base, while the liquid part is concentrated and then coated onto the paper. basically. This production process is largely just a physical reorganization of raw materials. As far as its chemical composition is concerned, the recombined tobacc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B15/20A24B15/28C12R1/01
Inventor 魏敏陈义坤罗诚浩李冉宋旭艳
Owner HUBEI CHINA TOBACCO IND
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