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Composite fermentation spice including tobacco leaf, trigonella foenum-graecum L., capsicum annuum and solanum lycopersicum and application thereof to recombinant tobacco leaf

A compound fermentation and fenugreek technology, which is applied in the fields of application, tobacco, and tobacco treatment, can solve the problems of uncomfortable taste, strong irritation, and weak smoke, and achieve the effect of enriching the aroma of cigarettes, improving the irritation, and reducing the irritation

Inactive Publication Date: 2013-10-09
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the taste-absorbing defects such as heavy miscellaneous gas, strong irritation, weak smoke and uncomfortable taste in the current recombined tobacco leaves, the present invention provides a compound fermented spice of tobacco leaves, fenugreek, bell pepper and tomato and its application in recombined tobacco leaves

Method used

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  • Composite fermentation spice including tobacco leaf, trigonella foenum-graecum L., capsicum annuum and solanum lycopersicum and application thereof to recombinant tobacco leaf
  • Composite fermentation spice including tobacco leaf, trigonella foenum-graecum L., capsicum annuum and solanum lycopersicum and application thereof to recombinant tobacco leaf

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Embodiment 1

[0032] The preparation method of the microbial Raoultella planticolaVP4-4 CCTCC M 2012005 biotransformation of the present invention to produce tobacco leaves and cocoa extract compound fermentation spices is as follows:

[0033] 1) Preparation of tobacco leaf extract: crush tobacco leaves to 100 mesh, then mix tobacco powder and water at a mass ratio of 1:30, reflux extraction for 3 hours and then filter, repeat reflux extraction filtrate once, and combine filtrates to obtain tobacco leaf extract , and sterilized for use;

[0034] 2) Preparation of fenugreek extract: mix fenugreek powder and water at a mass ratio of 1:40, magnetically stir, reflux for extraction for 3 hours, then filter, repeat reflux extraction once, and combine the filtrates after filtration to obtain fenugreek extract. and sterilized for use;

[0035] 3) Preparation of tomato and sweet pepper mixed extracts: slice tomatoes and sweet peppers to 1 cm in length and width, mix them at a mass ratio of 1:2, then ...

Embodiment 2

[0044] The preparation method of the microbial Raoultella planticolaVP4-4 CCTCC M 2012005 biotransformation of the present invention to produce tobacco leaves and cocoa extract compound fermentation spices is as follows:

[0045] 1) Preparation of tobacco leaf extract: crush tobacco leaves to 50 mesh, then mix tobacco powder and water at a mass ratio of 1:50, reflux extraction for 3 hours and then filter, repeat reflux extraction filtrate 3 times, and combine filtrates to obtain tobacco leaf extract , and sterilized for use;

[0046] 2) Preparation of fenugreek extract: mix fenugreek powder and water at a mass ratio of 1:20, magnetically stir, reflux extraction for 1 hour, then filter, repeat reflux extraction 3 times, and combine the filtrates after filtration to obtain fenugreek extract. and sterilized for use;

[0047] 3) Preparation of tomato and sweet pepper mixed extract: slice tomatoes and sweet peppers to 1 cm in length and width, mix with water at a mass ratio of 1:0...

Embodiment 3

[0057] The preparation method of the microbial Raoultella planticolaVP4-4 CCTCC M 2012005 biotransformation of the present invention to produce tobacco leaves and cocoa extract compound fermentation spices is as follows:

[0058] 1) Preparation of tobacco leaf extract: crush the tobacco leaves to 80 mesh, then mix the tobacco powder and water at a mass ratio of 1:40, reflux extraction for 3 hours and then filter, repeat the reflux extraction filtrate twice, and combine the filtrates to obtain the tobacco leaf extract , and sterilized for use;

[0059] 2) Preparation of fenugreek extract: mix fenugreek powder and water at a mass ratio of 1:30, magnetically stir, reflux extraction for 2 hours, then filter, repeat reflux extraction 3 times, and combine the filtrates after filtration to obtain fenugreek extract. and sterilized for use;

[0060] 3) Preparation of tomato and sweet pepper mixed extracts: slice tomatoes and sweet peppers to 1 cm in length and width, mix them at a mas...

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Abstract

The invention discloses a composite fermentation spice including tobacco leaf, trigonella foenum-graecum L., capsicum annuum and solanum lycopersicum and application thereof to a recombinant tobacco leaf. A strain is named raoultella planticola VP4-4 and is collected with the collection number CCTCC M 2012005, the full length of a 16SrRNA (16 ribosomal Ribonucleic Acid) sequence of the strain is 1,408bp, and the similarity between the 16SrRNA sequence and raoultella planticola is up to 99.563 percent. In a preparation method, a composite fermentation extract of extracts of tobacco leaf, trigonella foenum-graecum L., capsicum annuum and solanum lycopersicum is prepared from the strain by performing culture activation, preparation of a seed culture fluid, fermentation culture and transformation of a zymotic fluid. The fermentation spice is prepared from raw materials including original tobacco leaves of a cigarette product, a trigonella foenum spice which can be applied to cigarettes, capsicum annuum and solanum lycopersicum with a wide source, and has an industrial application prospect.

Description

technical field [0001] The invention relates to the technical field of additives for recombined tobacco leaves, in particular to a compound fermented spice of tobacco leaves, fenugreek, bell pepper and tomato and its application in recombined tobacco leaves Background technique [0002] Reconstituted tobacco leaves, also known as reconstituted tobacco leaves or homogeneous tobacco leaves, are mainly composed of tobacco powder, chips, tobacco stems or low-grade tobacco leaves with adhesives and other additives. The existing domestic papermaking thin sheet production process extracts the water-soluble substances in tobacco leaves and tobacco stems with water, and after solid-liquid separation, the solid part is pulped and then made into a paper base, while the liquid part is concentrated and then coated onto the paper. basically. The chemical components of the recombined tobacco leaves obtained by this method are not much different from those of tobacco leaves and tobacco ste...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A24B15/28C12R1/01
Inventor 罗诚浩魏敏陈义坤宋旭艳李冉
Owner HUBEI CHINA TOBACCO IND
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