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Preparation method and application of Bacillus methylotrophicus and tobacco spine date seed extract thereof

A technology of methanol-eating spores and jujube kernels, which is applied in the field of preparation of bacillus methanol-eating bacteria and jujube kernel extracts for tobacco, can solve the problems of environmental pollution, single aroma and taste of tobacco extracts, and insufficient aroma intensity, and achieve raw material The effect of wide sources, simple process, wide sources and easy access

Inactive Publication Date: 2013-12-18
HUBEI CHINA TOBACCO IND
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the defects that the extraction steps of chemically synthesized tobacco extract are complex, the environment is seriously polluted, the aroma and taste of the tobacco extract is single and the aroma intensity is still insufficient, the present invention provides a kind of Bacillus methylotrophicus, and the strain is named Bacillus methylotrophicus VJ4-1 was preserved in the Chinese Type Culture Collection Center of Wuhan University on January 11, 2012, with the preservation number CCTCC NO: M2012004. Its biological characteristics are: it is a Gram-positive bacterium that can grow in 10% NaCl, It has the activity of arginine dihydrolase and ornithine decarboxylase, which can produce acid and liquefy milk; Inositol, sucrose, maltose, D-sorbitol, L-arabinose, DL-lactate, L-alanine glycogen, L-proline histidine, citrate as carbon source medium growth, its 16S rRNA sequence is 100% similar to the 16S rRNA sequence of Bacillus methylotrophicus

Method used

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  • Preparation method and application of Bacillus methylotrophicus and tobacco spine date seed extract thereof
  • Preparation method and application of Bacillus methylotrophicus and tobacco spine date seed extract thereof
  • Preparation method and application of Bacillus methylotrophicus and tobacco spine date seed extract thereof

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Embodiment 1

[0035] The present invention utilizes the microorganism Bacillus methylotrophicus VJ4-1CCTCC NO: M 2012004 to biotransform the preparation method of Zianzaorex extractum for tobacco as follows:

[0036] 1) The jujube seed and water were refluxed and extracted at a mass ratio of 1:20 for 3 hours, then filtered, and the filtrate was sterilized at 115°C for 25 minutes;

[0037] 2) Slant culture: Inoculate Bacillus methanolivorus on the slant medium of potato dextrose agar, at 25°C and pH 10, culture it statically for 24 hours and then place it in the refrigerator;

[0038] Slant medium: potato infusion 200mL / L, glucose 20g / L, agar 13g / L;

[0039] 3) Preparation of seed culture medium: use the slant culture obtained in the above step 2) to inoculate the seed culture medium, vibrate for 12 hours at 25°C and rotate at 150 rpm, and transfer 4 times in succession to prepare the seed culture medium;

[0040] The composition of the seed liquid medium is: maltose 50g / L, peptone 10g / L, sod...

Embodiment 2

[0044] The present invention utilizes the microorganism Bacillus methylotrophicus VJ4-1CCTCC NO: M 2012004 to biotransform the preparation method of Zianzaorex extractum for tobacco as follows:

[0045] 1) The jujube seed and water were refluxed and extracted at a mass ratio of 1:30 for 2 hours, then filtered, and the filtrate was sterilized at 115°C for 25 minutes;

[0046] 2) Slant culture: inoculate Bacillus methanolivorus on the slant medium of potato dextrose agar, at 30°C and pH 7, let it stand for 36 hours and place it in the refrigerator;

[0047] Slant medium: potato infusion 200mL / L, glucose 20g / L, agar 13g / L;

[0048] 3) Preparation of seed culture solution: use the slant culture obtained in the above step 2) to inoculate the seed medium, vibrate and culture at 30°C and 120 rpm for 24 hours, and make the seed culture solution after continuous transfer for 3 times;

[0049] The seed liquid medium consists of: maltose 50g / L, peptone 10g / L, sodium chloride 5g / L;

[0...

Embodiment 3

[0053] The present invention utilizes the microorganism Bacillus methylotrophicus VJ4-1CCTCC NO: M 2012004 to biotransform the preparation method of Zianzaorex extractum for tobacco as follows:

[0054] 1) The jujube seed and water were refluxed and extracted at a mass ratio of 1:40 for 1 hour, then filtered, and the filtrate was sterilized at 115°C for 25 minutes;

[0055] 2) Slant culture: inoculate Bacillus methanolivorus on the slant medium of potato dextrose agar, at 35°C and pH 5, culture it statically for 72 hours and then place it in the refrigerator;

[0056] Slant medium: potato infusion 200mL / L, glucose 20g / L, agar 13g / L;

[0057] 3) Preparation of seed culture solution: use the slant culture obtained in the above step 2) to inoculate the seed medium, vibrate and culture at 35°C and rotate at 100 rpm for 36 hours, and make the seed culture solution after two consecutive transfers;

[0058] The seed liquid medium consists of: maltose 50g / L, peptone 10g / L, sodium chl...

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Abstract

The invention discloses a Bacillus methylotrophicus strain VJ4-1 of which the preservation number is CCTCC No:M 2012004. The invention provides a tobacco spine date seed extract prepared by using microbe Bacillus methylotrophicus and conducting biotransformation and an application of the spine date seed extract in tobacco. According to the invention, by fermenting the spine date seeds with the microbial strain, a natural flavor, i.e. the tobacco spine date seed extract can be produced; the method has the advantages of simple process, wide sources of main raw materials, and environmentally friendly and harmless production process, and has an industrial production prospect; the obtained natural flavor can be directly used for adding fragrance for cigarettes, is in harmony with the smoke, adds the sweet and fragrance of cream and fragrance of flower, makes the fragrance soft and comfortable, and improves the tasting, and can be mixed with other tobacco flavors.

Description

technical field [0001] The invention relates to the technical field of spices for tobacco, in particular to a preparation method and application of bacillus methanolate and jujube seed extract for tobacco. Background technique [0002] In tobacco products, the aroma and taste of tobacco are very important. Adding spices is a key link in the cigarette process, and it is also an effective means to improve the product style and improve the quality of cigarette aroma. Tobacco flavors can be divided into chemical synthetic flavors and natural flavors. In recent years, the research and development of natural plant flavors has been the focus and hotspot of ingredients because of their Chinese characteristics, natural harmlessness, and easy availability of raw materials. Applying microbial fermentation engineering to the biosynthesis of flavors and fragrances, using Chinese herbal medicine plants as raw materials, various natural flavors can be produced by using microorganisms, whi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A24B3/12C12R1/07
Inventor 魏敏罗诚浩宋旭艳陈义坤岳海波
Owner HUBEI CHINA TOBACCO IND
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