1035 results about "Drug biotransformation" patented technology

The invention provides methods for detecting variations in polymorphic sites and/or variations in gene copy number. The methods are particularly useful for analysis of biotransformation genes, such as cytochromes P450.

Methods for solubilizing algae or algal material are provided to facilitate the recovery of oil or lipids, as well as hydrocarbons and carbohydrates, from algae or algal material. The methods involve contacting algae or algal material with an oxoacid ester or thioacid ester of phosphorus or a mixture of an oxoacid of phosphorus and/or an alcohol to form a mixture thereof under conditions effective to solubilize the algae or algal material. These methods optionally further comprise bioconversion of the solubilized algae or algal material to form a composition suitable for recovery of oils and non-oil chemicals.

The invention discloses an in situ bioremediation method for strengthening the petroleum contaminated soil by biomass which belongs to the environment restoration technique. The method utilizes the biomass added with biomass degradation fungi to promote the conversion of biomass in polluted soil. The intermediate product transformed by biomass in soil is utilized to serve as the preferential carbon source of the petroleum hydrocarbon degradation complex bacteria for promoting the biological accumulation of the petroleum hydrocarbon degradation complex bacteria; meanwhile, the imposed petroleum hydrocarbon degradation complex bacteria contains the fungus that can produce biological surface active agent efficiently, the fungus produces abundant biological surface active agent during growth to activate the petroleum hydrocarbon pollutants in polluted soil for increasing the biological utilize degree, thus promoting the biotransformation efficiency of the petroleum hydrocarbon degradation complex bacteria to pollutants. Humus of the transformation product of the biomass in the soil can also improve the granular structure of the soil, and increase the fertility of the soil, so the polluted soil can be restored. The method is simple and is easy to operate and promotion.

Sterile nanoemulsions are prepared containing oily droplets of a diameter of less than 100 nm in an aqueous phase. The oily droplets contain a lipophilic substance, and have on their surface an amphoteric emulsifier in an amount of preferably 0.65 to 0.75 parts by weight amphoteric emulsifier for one part by weight of oily component forming the droplets. The oily component of the droplets is preferably a triglyceride containing oleic acid and/or linoleic acid. The nanoemulsions are used for delivering into cells the lipophilic substance contained by the oily droplets. This delivery can be used to biotransform the lipophilic substance, promote cell differentiation, growth or biosynthesis of a desired substance, or to test for toxicity of the lipophilic substance.

The invention discloses a method for culturing rare edible fungi including tricholoma lobayense heim and clitocybe maxima by using vine shoot dust. The method comprises the following steps of: A, preparation of a fermentation culture solution; B, preparation of liquid spawn; C, preparation of granular spawn; D, preparation of a culture medium: selecting 68-72 percent of vine shoot dust, 8-12 percent of cotton seed hull, 8-12 percent of broken dry broad-leaved tree fallen leaves, 8 percent of bran, 1 percent of sucrose and 1 percent of calcined lime in a weight ratio, stirring in a material-water ratio of 1:1.4, bagging, performing conventional sealing, and sterilizing for later use; E, inoculating culture; and F, harvesting. The vine shoot is treated and developed into a raw material for culturing the rare edible fungi including the tricholoma lobayense heim and the clitocybe maxima, a natural culture medium is provided for the fungi culture industry, the biotransformation utilization of the vine shoot is realized, and the culture costs of the tricholoma lobayense heim and the clitocybe maxima are reduced.

A biological dehydrogenation preparation method of steroid drug intermediate takes the formula (1) compound as a substrate, and adopts an arthrobacter simplex biological dehydrogenation method to obtain formula (2) compound; the special process comprises the following steps: smashing or dissolving the formula (1) compound with solvent, putting into a fermentation tank in which arthrobacter is cultivated for biotransformation, extracting, separating and refining, and drying to obtain dehydrogenated polymer, i.e. compound (2); and the dehydrogenation transformation rate can reach 70 to 90 percent.

The present invention is the microbe fermenting process of producing perfume phenylethanol with tobacco material. The technological process includes using tobacco as precursor for biological conversion, adding proper culture medium, selecting proper bacteria strain, fermentation culture in certain technological conditions., degrading the lignin, pectin and polyphenol compounds to convert into phenylethanol and final purifying via extraction or ion exchange resin adsorption. The obtained phenylethanol product has pure fragrance and may be used for food product. The production process is simple and has wide application foreground.

The invention relates to a production method of selenium-enriched pork. A feed fed when live pigs are bred is a selenium-enriched nutritional feed processed by corn, wheat bran, soybean meal, fish meal, feed grade calcium hydrophosphate, mountain flour, trace elements and selenium-enriched ryegrass hay, wherein the content of the hay in the feed is not lower than 15wt%, and the selenium content in the hay is not less than 1,000mug/kg. The selenium-enriched ryegrass hay is obtained by drying ryegrass the reaped or harvested leaf surfaces of which are sprayed and applied with a sodium selenite nutrient solution the concentration of which is not lower than 200ppm. In the invention, the selenium-enriched pork is obtained by the two-time biotransformation of inorganic selenium and has ruddy meat color, tender quality, fragrant taste and no any fishy smell of a pigpen, the selenium content in each kilogram of pork is not less than 80mug, and the lowest selenium demand of a human body can bemet as long as an adult eats more than 100g of selenium-enriched pork every day.

The invention provides a culture medium for cultivating coprinus comatus, which is composed of chestnut shells, crop straws or corncobs, dry livestock manure, calcium superphosphate, quick lime, enzyme bacterium and water. The method utilizing the culture medium to cultivate the coprinus comatus comprises the following steps: first, uniformly stirring all raw materials to produce a base material; second, stacking the base material for fermentation; third, bagging the fermented base material for inoculation so as to produce bacteria sticks; fourth, placing the bacteria sticks in a bacteria cultivating room; fifth, debagging the bacteria sticks so as to allow the bacteria sticks to be stood in a bed and is covered with soil; sixth, carrying out water spray management; and seventh, picking the coprinus comatus. According to the invention, the chestnut shells are basic material of the culture medium which is green and pollution-free, the cultivating process is simple, and the production cost is low; and the coprinus comatus is high in yield, good in quality, and high in biotransformation efficiency.

The invention discloses a preparation method of a porous inorganic filling materials-fixed quinone compound, belonging to the technical field of water treatment in environmental engineering. The method comprises the following steps of: selecting porous inorganic filling materials such as ceramsites, volcanics and the like; plating gamma-aluminum oxide on the surface of the materials; aminating, so that the surfaces of the porous inorganic filling materials contain a certain quantity of primary amidogen; putting the aminated materials into hydrochloric solution to have reaction for protecting the primary amidogen; and putting the reacted porous inorganic filling materials into sodium hydroxide solution, adding sulfonyl chloride group-containing anthraquinone compound which is dissolved in dichloromethane, and reacting for 6-10h under the room temperature to fix the water-soluble quinone compound. The quinone compound-containing macroporous polymer is applied to an anaerobic reactor to be capable of improving the biotransformation speed of an organic matter which is hardly degraded. The method covalently fixes the quinone compound on the macroporous polymer of a biologic carrier, so that the redox medium is easily contacted with the microbe, thereby overcoming the problem of the secondary pollution since the water-soluble quinone compound flows out with the water.

The invention discloses a method for preparing a macroporous polymer fixed quinonoid compound and belongs to the technical field of water treatment in environment engineering. The method is characterized by selecting hydroxyl group containing macroporous polymers for aminating treatment so as to make the macroporous polymers contain primary amino groups in certain quantity. At the room temperature, the macroporous polymers are added in solution of sodium hydroxide, then anthraquinone compounds, which are dissolved in methylene chloride and contain sulfonic acid chloride groups, are added, andthe mixed solution stirred for reaction for 0.5 to 2 hours, so that the fixture of the quinonoid compounds are realized. The macroporous polymers containing quinonoid compounds can be applied to an anaerobic reactor to improve the biological conversion rate of hardly degradable organic matters. The method has the advantages that: the quinonoid compounds are covalently fixed on a biological carrier which is macroporous polymers, so that a redox mediator is more easily contacted with microorganisms; and the problem of secondary pollution caused by emission of water soluble quinonoid compounds along with discharge water is solved.

Stevioside is a novel sweeter which is extracted from stevia leaves, and the stevioside in the stevia leaves mainly comprises two components of stevioside and rebaudioside A. The sweetness and mouthfeel of the rebaudioside A are both excellent, while the stevioside has certain lingering bitterness; and as the structure and nature of the rebaudioside A are approximate to the structure and nature of the stevioside, the separation of rebaudioside A and stevioside features high cost and great technical difficulty. In the invention, microorganisms are transformed for 20h-150h under the conditions that the concentration of the stevioside is 0.01%-5% (W/W), the temperature is 25-80 DEG C, the quantity of inverting enzyme or bacterial solution is 0.1%-10% (W/W), and the pH value is 2.0-7.0, and the stevioside contained in the rebaudioside A solution can be selectively degraded, thus obtaining high purity rebaudioside A containing no stevioside. The biotransformation method used in the invention has temperate conditions, does not use toxic methanol and other chemicals or macroporous resin with high cost, thus being a novel technology with safety, greenness and environmental friendliness.

The invention concerns a method for producing dicarboxylic acids (DCA) with long hydrocarbon chains, also called diacids, which consists in culturing a mutant strain of Yarrowia lipolytica obtained by mutagenesis directed and more particularly disrupted at least for the POX2, POX3, POX4 and POX5 genes encoding acyl-CoA oxydase, in a medium consisting essentially of an energetic substrate including at least one carbon source and one nitrogen source and in subjecting said strain to a bioconversion substrate selected among n-alkanes of at least 10 carbon atoms, fatty acids of at least 10 carbon atoms, their alkyl esters and natural oils.

The invention relates to selenium-rich bean sprouts and a production process thereof. The production process is to use sprouting of soybeans to perform the biotransformation of selenium to obtain a bean sprout product rich in organic selenium. The invention belongs to the field of deep processing of farm products, and also belongs to the field of the production of functional food. The production process comprises the following steps of: performing selection and impurity removal on the soybeans, then soaking in solution of sodium selenite, draining water, and then ensuring that the soybeans sprout in a mode of water spraying cultivation to obtain the selenium-rich bean sprouts.

The invention relates to an artificial cultivation method of lucid ganoderma, in particular to an artificial cultivation method capable of realizing highland cultivation of wild ganoderma tsugae murr. The artificial cultivation method comprises the following steps: (1), a stock culture is prepared; (2), a pre-culture spawn is prepared; (3), a production spawn is prepared; (4), inoculating and cultivation are conducted; and (5), ganoderma tsugae murr growing management is conducted till a white edge of a fungi pileus is turned to yellow completely, and then the ganoderma tsugae murr is picked. According to the artificial cultivation method, the total biotransformation rate can reach 55-85%; the taste of the obtained ganoderma tsugae murr is relatively bitter; the ganoderma tsugae murr has the taste of ganoderma lucidum, and has effects of improving the immunity, preventing a cancer and the like; and requirements of the ganoderma tsugae murr in a wide range can be met through the artificial cultivation.

The invention discloses a production method and strain of gamma-aminobutyric acid (GABA), belonging to the technical field of gene engineering. The method comprises the following steps of: introducing a glutamate decarboxylase gene into a glutamic acid production bacterium to construct a gene engineering bacterium; and removing a carboxyl from a self-accumulated glutamic acid by using glutamate decarboxylase secreted by the gene engineering bacterium to synthesize the GABA. In the invention, fermentation of glutamic acid is combined with transformation of the GABA, so that the production steps of the GABA are simplified; moreover, glucose, urea and the like are taken as culturing raw materials, so that the production cost of the method disclosed by the invention is remarkably lowered compared with a biological transformation method in which L-glutamic acid or L-sodium glutamate serving as a precursor needs to be exogenously added.

The invention provides a process for biotransformation of kitchen waste through hermetia illucens. The process includes the following steps that ingredients are added into the sorted and smashed kitchen waste, the relative humidity is adjusted, processing materials are obtained, EM bacteria are added, and a mixture is obtained after even mixing is carried out; and the mixture is paved on a cultivation disc, five-day-age larva of the hermetia illucens is added, feeding is carried out at the temperature ranging from 28 DEG C to 32 DEG C and the relative humidity ranging from 70% to 90%, and after feeding is completed, hermetia illucens mature larva and remnant materials are separated. According to the process, one-time putting of the kitchen waste can be achieved, the growth requirement of the hermetia illucens larva can be met, the processing process can be greatly simplified, and efficiency is improved.

The invention relates to saccharification processing technology of jerusalem artichoke by utilizing high-quality fructose biomass as a raw material and strain selection and technology optimization for producing mannitol by taking jerusalem artichoke as carbon source through fermentation. The method comprises the following steps: 1) crushing jerusalem artichoke tuber into coarse particles, filtering after water leaching and enzymolysis for 6 hours, supernating at 42 DEG C, rotating, evaporating and concentrating to obtain saccharification jerusalem artichoke juice with high concentration of fructose; 2) establishing high performance liquid chromatography analysis and detection conditions which can synchronously analyze the content of fermentation liquor substrate (glucose and fructose) and products (mannitol); and 3) inspecting the capacity for producing lactic acid and mannitol through fermentation by seven lactic acid bacteria by utilizing saccharification jerusalem artichoke juice with different concentration of total sugar, thus determining lactic acid bacteria with high transformation rate and production intensity of fructose, and optimizing production fermentation conditions and the highest concentration of tolerant substrate. Through feed-batch fermentation, production efficiency can be improved and mannitol can be continuously produced in large scale. The method not only generates no byproduct of sorbitol, but also has low production cost, wide raw material sources, simple technology, and mature technical route and can be implemented in industrialization.

The invention discloses a composite bacterial agent and a biological method for treating flow-back fracturing fluid to obtain oil displacement active water, which relate to biotransformation and reutilization technology of fracturing flow-back fluid. In the method, the fracturing flow-back fluid is treated by a microbial fermentation method, and a residual organic substance harmful to environment in the flow-back fluid is transformed into biological active water with certain function. The composite bacterial agent and the method are applied to reinjection water of water injection wells, reinjection water for single-well soaking, reinjection water for oil field blockage removal, reinjection water for other purposes, water for medicament preparation in a technical process and the like. The composite bacterial agent comprises biosurfactant, organic acid, organic ester, organic ketone, biogas and other metabolites produced by microbial metabolism of the fracturing flow-back fluid.