Microbe fermenting process of producing perfume phenylethanol

Abstract

The present invention is the microbe fermenting process of producing perfume phenylethanol with tobacco material. The technological process includes using tobacco as precursor for biological conversion, adding proper culture medium, selecting proper bacteria strain, fermentation culture in certain technological conditions., degrading the lignin, pectin and polyphenol compounds to convert into phenylethanol and final purifying via extraction or ion exchange resin adsorption. The obtained phenylethanol product has pure fragrance and may be used for food product. The production process is simple and has wide application foreground.

Description

technical field

[0001] The invention relates to the field of bioengineering and technology, in particular to a process for producing spices by using tobacco as a raw material through microbial fermentation. Background technique

[0002] The chemical name of phenylethanol is 2-phenylethanol or β-phenylethanol. It is a colorless viscous liquid, slightly soluble in water, and miscible with ethanol and oil. Phenylethyl alcohol is the most important and widely used aromatic compound in fragrances. Because of its soft, pleasant and long-lasting rose fragrance, it is widely used in various floral fragrances such as orange blossom, hyacinth and clove. It can also be used to prepare a variety of food flavors and tobacco flavors. Phenylethyl alcohol exists in many natural essential oils, and currently the products are mainly obtained through organic synthesis or extraction from natural products.

[0003] U.S. Patent No. 5,919,991 (1999) discloses a method f...

Examples

Embodiment 1

[0045] Candida utilis was used to carry out liquid aerobic fermentation of tobacco, and the specific fermentation process was as follows:

[0046] Seed culture:

[0047] The composition of seed medium is peptone 2% (percentage by weight, the same below), yeast extract 1%, glucose 2%, inorganic salt: FeSO 4 、KH 2 PO 4 , KCl, MgSO 4 , CaCl 2 Both are 0.1%, placed in a 200 ml culture bottle, added to 100 ml with distilled water, adjusted to PH=6.0 with oxalic acid or oxalate, and added to the Candida utilis strain after sterilization (cooling to room temperature) , carry out seed culture according to conventional methods, obtain liquid Candida utilis strain (being called for short liquid yeast strain), stand-by.

[0048] Shake flask fermentation of tobacco:

[0049] The formula of the culture medium for shake flask fermentation is as follows: Take 50g / L of tobacco (or tobacco stems, tobacco dust) that is pulverized to 20-40 meshes, 20g / L of glucose (or any one of other suga...

Embodiment 2

[0053] In addition to using Saccharomyces cerevisiae, which is a strain. All the other processing conditions are with embodiment 1. After the fermentation was finished, samples were taken to measure the content of phenylethyl alcohol in the fermentation broth with a GC-MS instrument, and the results are shown in Table 2.

[0054] Equally as seen from table 2: preferred fermentation condition is:

[0055] The inoculum size is preferably 10% (V / V), the fermentation temperature is 30°C, and the fermentation time is preferably 48 hours. At this time, the content of phenylethyl alcohol in the fermentation broth can reach 50ml / L.

Embodiment 3

[0057] Except for adopting Kluyveromyces chinensis as the strain, all the other technological conditions are the same as in Example 1.

[0058] After the fermentation was finished, samples were taken to measure the content of phenylethyl alcohol in the fermentation broth with a GC-MS instrument, and the results are shown in Table 3.

[0059] As can be seen from Table 3, the suitable fermentation conditions are:

[0060] 10% (V / V) of the inoculum size is good, the fermentation temperature is 30° C., and the fermentation time is preferably 48 hours. At this time, the content of phenylethyl alcohol in the fermentation broth can reach 46ml / L.

[0061] From Examples 1 to 3, it can be seen that under the same conditions, when Saccharomyces cerevisiae is used as the strain, better technical effects can be obtained. For this reason, just take Saccharomyces cerevisiae as bacterial classification and carry out scale-up experiment to further clarify content of the present invention belo...

technical field

[0001] The invention relates to the field of bioengineering and technology, in particular to a process for producing spices by using tobacco as a raw material through microbial fermentation. Background technique

[0002] The chemical name of phenylethanol is 2-phenylethanol or β-phenylethanol. It is a colorless viscous liquid, slightly soluble in water, and miscible with ethanol and oil. Phenylethyl alcohol is the most important and widely used aromatic compound in fragrances. Because of its soft, pleasant and long-lasting rose fragrance, it is widely used in various floral fragrances such as orange blossom, hyacinth and clove. It can also be used to prepare a variety of food flavors and tobacco flavors. Phenylethyl alcohol exists in many natural essential oils, and currently the products are mainly obtained through organic synthesis or extraction from natural products.

[0003] U.S. Patent No. 5,919,991 (1999) discloses a method f...

Examples

Embodiment 1

[0045] Candida utilis was used to carry out liquid aerobic fermentation of tobacco, and the specific fermentation process was as follows:

[0046] Seed culture:

[0047] The composition of seed medium is peptone 2% (percentage by weight, the same below), yeast extract 1%, glucose 2%, inorganic salt: FeSO 4 、KH 2 PO 4 , KCl, MgSO 4 , CaCl 2 Both are 0.1%, placed in a 200 ml culture bottle, added to 100 ml with distilled water, adjusted to PH=6.0 with oxalic acid or oxalate, and added to the Candida utilis strain after sterilization (cooling to room temperature) , carry out seed culture according to conventional methods, obtain liquid Candida utilis strain (being called for short liquid yeast strain), stand-by.

[0048] Shake flask fermentation of tobacco:

[0049] The formula of the culture medium for shake flask fermentation is as follows: Take 50g / L of tobacco (or tobacco stems, tobacco dust) that is pulverized to 20-40 meshes, 20g / L of glucose (or any one of other suga...

Embodiment 2

[0053] In addition to using Saccharomyces cerevisiae, which is a strain. All the other processing conditions are with embodiment 1. After the fermentation was finished, samples were taken to measure the content of phenylethyl alcohol in the fermentation broth with a GC-MS instrument, and the results are shown in Table 2.

[0054] Equally as seen from table 2: preferred fermentation condition is:

[0055] The inoculum size is preferably 10% (V / V), the fermentation temperature is 30°C, and the fermentation time is preferably 48 hours. At this time, the content of phenylethyl alcohol in the fermentation broth can reach 50ml / L.

Embodiment 3

[0057] Except for adopting Kluyveromyces chinensis as the strain, all the other technological conditions are the same as in Example 1.

[0058] After the fermentation was finished, samples were taken to measure the content of phenylethyl alcohol in the fermentation broth with a GC-MS instrument, and the results are shown in Table 3.

[0059] As can be seen from Table 3, the suitable fermentation conditions are:

[0060] 10% (V / V) of the inoculum size is good, the fermentation temperature is 30° C., and the fermentation time is preferably 48 hours. At this time, the content of phenylethyl alcohol in the fermentation broth can reach 46ml / L.

[0061] From Examples 1 to 3, it can be seen that under the same conditions, when Saccharomyces cerevisiae is used as the strain, better technical effects can be obtained. For this reason, just take Saccharomyces cerevisiae as bacterial classification and carry out scale-up experiment to further clarify content of the present invention belo...