34 results about "Kluyveromyces" patented technology

The invention provides a genetically engineered bacterium for producing polydeoxyribonucleotide as well as a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium comprises kluyveromyces marxianus for expressing a salmon DNA polymerase I gene. According to the invention, the edible probiotic Kluyveromyces marxianus is transformed through genetic engineering, efficient, safe and controllable production of PDRN is realized, the biological activity of PDRN is equivalent to that of PDRN from a natural source, and large-scale production and application of PDRN are facilitated.

This invention relates to the Kluyveromyces marxianus strain and its use, wherein the Kluyveromyces marxianus strain is Kluyveromyces marxianus AMCC30648, deposited with the China Center for the Preservation of Typical Cultures (CCTCC) with deposit number CCTCC NO:M 20222108, or Kluyveromyces marxianus AMCC31343, deposited with the China Center for the Preservation of Typical Cultures (CCTCC) with deposit number CCTCC NO:M 20222111. The Kluyveromyces marxianus strain is characterized by producing a variety of flavor substances at high concentrations. It can be used in fields such as brewing, beverages, and bakeries.

This invention provides a feed additive and compound feed for preventing and treating coccidiosis in broilers, comprising the following components: *Kluyveromyces martensii*, ferulic acid esterase, aminopeptidase, and glucanase. Preferably, the mass percentage of *Kluyveromyces martensii* is 30-40%; the mass percentage of ferulic acid esterase is 15-28%; the mass percentage of aminopeptidase is 15-25%; and the mass percentage of glucanase is 18-25%. Based on extensive research, this invention has determined that this combination has a significant effect on coccidiosis control in broilers, effectively improving broiler production performance indicators, increasing economic benefits, and reducing the use of anticoccidial drugs.

The invention provides a C3-2 strain for producing bactrian camel chymosin and application of the C3-2 strain, and belongs to the technical field of biological engineering. According to the present invention, the Latin of the C3-2 strain is Kluyveromyces lactis, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20251316. According to the method, the C3-2 strain is used for fermentation, the fermentation liquor is concentrated by adopting an ultrafiltration method, small molecular impurities are removed, and the bactrian camel chymosin with high enzyme activity is obtained by combining affinity chromatography. The cheese prepared from the bactrian camel chymosin obtained by the preparation method disclosed by the invention is high in crosslinking degree, harder and finer in texture, smaller, more uniform and more compact in microstructure texture, lower in bitter taste and higher in overall volatile flavor substance content, and the overall evaluation is superior to that of cheese prepared from commercial chymosin.

This invention relates to the field of food fermentation technology, specifically to a high-efficiency fermentation process for purple sweet potato rice wine. It aims to solve the technical problems of significant anthocyanin degradation and fading, difficulty in balancing flavor and efficiency, poor strain adaptability, and short shelf life during the fermentation of purple sweet potato rice wine. This invention improves product quality and fermentation efficiency by constructing a specialized compound fermenting agent and employing a two-stage fermentation process with dynamic pH control. The compound fermenting agent consists of *Rhizopus oryzae*, *Monascus purpureus*, *Kluyveromyces martensii*, and *Lactobacillus plantarum*. The fermentation process employs two stages: the first stage is aerobic fermentation, which promotes the stable existence of anthocyanins and facilitates enzyme activity; the second stage is anaerobic fermentation, which further stabilizes the color and inhibits unwanted microorganisms. This invention uses ultra-high pressure sterilization combined with post-treatment with natural antioxidants to sterilize and preserve the purple sweet potato rice wine, which can improve the pigment retention rate and significantly extend the shelf life of the purple sweet potato rice wine.

The invention relates to the technical field of minced fillet deodorization, and discloses a minced fillet deodorization process for fish ball production, which comprises the following steps: (1) cleaning and dehydrating a minced fillet raw material, and controlling the water content at 80-95% after the raw material is dehydrated to obtain a primary minced fillet raw material; (2) activating a composite fungus material, adding the activated composite fungus material into the primary minced fillet raw material, uniformly stirring, fermenting at 28 DEG C for 50-60 minutes, heating to 30 DEG C, and fermenting for 50-60 minutes; wherein the composite fungus material comprises rhizomucor miehei, kluyveromyces marxianus, lactococcus lactis and saccharomyces cerevisiae; and (4) heating the minced fillet obtained in the step (3) to 65-68 DEG C, keeping the temperature for 2-3 minutes, rinsing with ice water, and dehydrating to obtain the fishy smell removed minced fillet. According to the method, the effect of removing the fishy smell of the minced fillet can be improved.

This invention relates to the field of microbial directed fermentation processing technology for yak milk fat products, and discloses a compound microbial agent for yak butter fermentation and its application. The compound microbial agent includes *Lactococcus gasseri* (…). Lactococcus garvieae CGMCC No. 32756, Enterococcus faecium ( Enterococcus faecium CGMCC NO.36378 and Kluyveromyces martensii ( Kluyveromyces Marxian (CGMCC NO.39019) All three strains exist in live form. This invention also discloses a method for preparing the composite microbial agent, a method for fermenting yak ghee using the composite microbial agent, and the resulting fermented yak ghee. The three strains possess environmental tolerance and single-strain growth characteristics suitable for optimizing the synergistic fermentation process of yak ghee; the interaction of the three strains can enhance the growth performance of the strains. Inoculating the composite microbial agent into yak milk fat substrate for fermentation can improve the flavor of yak ghee, reduce acid value, peroxide value, and biogenic amine accumulation, and improve the controllability of the yak ghee fermentation process and the stability of product quality.

This invention relates to the field of microbial technology, specifically to Kluyveromyces martensii YF51-1 and its application in semi-dry fermented whey wine. This strain can efficiently ferment lactose and galactose, while also exhibiting a high ABTS scavenging rate. The fermentation broth contains a high amount of amino acid nitrogen. Under conditions where sucrose is present, the amino acid nitrogen content in the sweetened whey fermentation broth is significantly higher than that in the unsweetened whey fermentation broth. Through process optimization, the optimal fermentation conditions for strain YF51-1 were obtained, ultimately producing a semi-dry whey fermented wine with a rich aroma and a sweet and sour taste, achieving an amino acid nitrogen content of 0.337 g / L, significantly higher than existing technologies. Through optimization of the freeze-drying process, a freeze-drying protectant suitable for strain YF51-1 was derived. This protectant consists of mannose, corn steep liquor powder, lysine, and calcium chloride. After optimization, this protectant demonstrates good protective effect on the strain, with a survival rate of up to 97.22%.

The invention discloses kluyveromyces marxianus grx168 and application thereof, the kluyveromyces marxianus is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on June 9, 2025, and the preservation number is CGMCC NO.34816. The kluyveromyces marxianus grx168 can be used for preparing the kluyveromyces marxianus. The strain is screened from Kefir grains in Xinjiang, and a nerve cell cAMP experiment verifies that the opioid activity of a fermentation product of the strain is obviously higher than that of other yeast strains, and the cAMP content of the fermentation product of the strain is obviously lower than that of a blank control. The strain can be used for preparing fermented food or health care products with opioid activity, and a high-quality strain resource is provided for development of functional fermented products.

This invention discloses a method for biotechnology-based conversion of livestock and poultry manure into animal feed, belonging to the field of agricultural waste resource utilization. The method includes: solid-liquid separation and crushing of livestock and poultry manure, adjusting the moisture content to 65%~75%; applying a pulsed electric field to treat the manure, resulting in a microbial cell breakage rate ≥70% and a cellulose crystallinity reduction ≥15%; adding a complex enzyme preparation composed of laccase, manganese peroxidase, xylanase, and lysozyme for enzymatic hydrolysis; after cooling, inoculating a mixed flora of thermophilic Bacillus stearothermophilus and Kluyveromyces martensii acclimated with sodium acetate and phenol; conducting a two-stage fermentation, with an aerobic stage followed by closed, static fermentation; and finally, low-temperature vacuum drying and pulverization to obtain feed protein powder. This invention, through the synergistic effect of pulsed electric field, complex enzyme, and acclimated microbial flora, achieves efficient, safe, and rapid feed utilization of livestock and poultry manure, with a total processing time ≤72 hours, a crude protein content of 28%~35%, and antibiotic residue ≤0.2 mg / kg.

This invention provides a natto fermentation composition and its application, belonging to the field of natto fermentation technology. The natto fermentation composition provided by this invention includes *Lactobacillus casei*, *Kluyveromyces lactis*, and *Bacillus natto*. The natto fermentation composition provided by this invention significantly increases nattokinase activity while reducing ammonia odor, making the fermented natto more acceptable to consumers. The natto fermented using the natto fermentation composition and method of this invention has a nattokinase activity as high as 2620.43±4.05 U / mL, and the natto fermented by this invention has a low volatile basic nitrogen content and a high sensory evaluation.

The invention relates to the technical field of wine brewing, in particular to a microbial agent and a method for alcohol-malolactic co-fermentation of dry red wine. According to the method, lactobacillus brevis LB-21 and pichia kluyveromyces PK-19 are selected, and malic acid-lactic acid fermentation is synchronously completed in the alcoholic fermentation process. Compared with the traditional sequential fermentation, the method can effectively shorten the fermentation period, and has obvious advantages in the aspects of color and aroma: in the aspect of color, the proportion of polymerized anthocyanin is increased, and the color stability is enhanced; in the aspect of aroma, the accumulation of acetate, fatty acid ethyl ester and phenethyl compounds is promoted, so that the grape wine presents richer flower and fruit aroma. The alcohol-malolactic co-fermentation method established by the invention is stable in fermentation process, can significantly improve the overall quality of the dry red wine, and has a good application prospect.

The present application relates to the technical field of bioengineering, and particularly relates to a Kluyveromyces marxianus strain and application thereof in fermentation production of ethanol. The present application provides a Kluyveromyces marxianus strain huJ01, and the preservation number of the strain is CGMCC No.22931. The strain can still maintain good growth and ethanol synthesis capacity under stress conditions such as high temperature, high acid, high ethanol and high salt. Under the optimal conditions of inulinase activity, the ethanol yield can reach 2.41%vol after 20% inoculation and static culture at 60 DEG C for 120 h. The strain is inoculated at 4% in a high stress culture medium containing 11.4% ethanol, pH 3.5 and 3.51% NaCl, and is statically cultured at 40 DEG C for 12 h, and the concentration of the bacterial liquid is significantly increased or not significantly decreased. It is shown that the strain still has strong ethanol fermentation performance under extreme environment, and is suitable for special industrial fermentation scenes such as high salt, high acid and high ethanol.

This invention discloses a plant-based compound fermented beverage that naturally enriches γ-aminobutyric acid (GABA) and possesses antioxidant functions, along with its preparation method. Addressing the issues of limited functionality and low utilization rate of plant active ingredients in existing GABA products, this invention provides an integrated biotransformation method. Using a variety of plants such as yam, gastrodia elata, kudzu root, and poria cocos as raw materials, and after specific proportions, a two-stage synergistic fermentation process is employed: aerobic fermentation with *Kluyveromyces martensii* followed by anaerobic fermentation with compound lactic acid bacteria. The fermentation endpoint is precisely controlled using alcohol content and pH values ​​as indicators. This method can simultaneously and efficiently convert and enrich precursor substances in the raw materials into various active substances such as GABA, flavonoids, and phenols. The fermented beverage product prepared by this invention shows significantly higher GABA, total flavonoid content, and SOD activity compared to the raw materials. The components work synergistically, exhibiting both sleep-aiding potential and antioxidant functions, without the addition of additional functional substances, thus meeting the market demand for high-end natural functional beverages.

The application belongs to the technical field of biological agents, and discloses a compound probiotic for preventing and treating acute intestinal inflammation of mice and application. The compound probiotic comprises: Kluyveromyces marxianus C2 with a preservation number of CCTCC NO: M 2014059, Bacillus subtilis KC with a preservation number of CCTCC NO: M 20211444, and Lactobacillus acidophilus. The applicant finds that the compound probiotic preparation can significantly relieve the clinical symptoms and the pathological damage of the colon tissue caused by DSS, significantly reduce the DAI score, and significantly improve the intestinal barrier function by constructing an IBD animal model and treating the mice by gavage with the compound probiotic.

The invention relates to the technical field of food processing, in particular to a preparation method of fermented oat flour with high digestibility, and the fermented oat flour is combined with kluyveromyces marxianus fermentation through double enzymolysis, so that the protein digestibility is improved. According to the method disclosed by the invention, a high-integration-degree continuous process path of front-end enzymolysis, two-stage fermentation at different temperatures and rear-end drying is realized through a strategy of separating enzymatic hydrolysate into supernate and precipitate and respectively applying the supernate and the precipitate to yeast propagation and secondary fermentation of thalli.

This invention discloses a strain of *Corynebacterium lucida* CYPJ1 and its applications. The *Corynebacterium lucida* CYPJ1 has the preservation number CGMCC No. 35719, and its saccharifying enzyme activity is 73.19 ± 0.09 U / mL. Using homogenized raw materials and fermentation with this strain results in increased soluble dietary fiber content and enhanced physical and functional properties. Foods or health products fermented with homogenized raw materials and this strain have lipid-lowering effects. This invention also found that using *Corynebacterium lucida* (…) after homogenization of raw materials… Clavispora lusitaniae CYPJ1 and Kluyveromyces macrocephala ( Kluyveromyces marxianus SXJ2 or Bacillus sicca ( Bacillus siamensis The DL-RXL01 combination fermentation yields even better results.

This invention discloses a genetically engineered strain of *Kluyveromyces martensii* and its applications, belonging to the field of bioengineering technology. The invention discloses a genetically engineered *Kluyveromyces martensii* strain, firstly obtained through screening a strain that produces high levels of ergosterol; based on this, it induces and isolates... Eye A haploid strain was constructed and then metabolically optimized through the introduction of heterologous DHCR24 and DWF5 genes, weakening of the competitive pathway gene ERG6, increasing the metabolic flux of the post-squalene pathway during sterol synthesis, peroxisome compartmentalization engineering of the mevalonate pathway, knockout of the hypoxia-inhibiting transcription factor ROX1, improved cofactor supply, and optimization of the yeast lipid pathway, resulting in a high-cholesterol-producing engineered strain. Through fed-batch fermentation, efficient and stable cholesterol synthesis was achieved, with a yield of 7.64 g / L, laying the foundation for cholesterol biomanufacturing.

The invention discloses a preparation method and application of camel milk with hypoglycemic activity, and belongs to the technical field of animal product processing. According to the research, lactobacillus bulgaricus, streptococcus thermophilus and kluyveromyces marxianus are used for carrying out microbial fermentation treatment on camel milk, and the optimal camel milk fermentation process is screened out by detecting various indexes such as texture characteristics, water holding stability, sensory evaluation, enzyme inhibition activity and protein hydrolysis degree; the fermented camel milk with potential hypoglycemic activity is prepared, the inhibitory activity of the camel milk to DPP-IV enzyme, alpha-amylase and alpha-glucosidase before and after digestion is detected in combination with an in-vitro simulated digestion experiment, and the prepared camel milk has high inhibitory activity to DPP-IV enzyme, alpha-amylase and alpha-glucosidase.

The invention provides a tandem RNA (Ribonucleic Acid) element capable of enhancing protein synthesis efficiency. The nucleic acid construct disclosed by the invention is formed by connecting a section of sequence rich in adenine deoxynucleotide in a 5'untranslated region of a PAB1 gene derived from yeast (such as saccharomyces cerevisiae or kluyveromyces yeast), an omega sequence, an oligomeric chain [oligo (A)] n of adenine deoxynucleotide and a translation initiation codon in series. When the nucleic acid construct is applied to a yeast in-vitro biosynthesis system (such as a kluyveromyces in-vitro protein synthesis system), the protein synthesis efficiency can be remarkably improved.

This invention belongs to the field of veterinary biological agent technology, specifically relating to a bacterial-enzyme complex preparation for the prevention and treatment of transmissible gastroenteritis (TGEV) in pigs, its preparation method, and its application. The bacterial-enzyme complex preparation comprises microcapsule particles; the core material of each microcapsule particle includes a complex of probiotics and a synergistic enzyme system; the complex of probiotics includes *Lactobacillus rhamnosus*, *Bacillus coagulans*, *Pediococcus pentosus*, and *Kluyveromyces marsupialis*. In the bacterial-enzyme complex preparation for the prevention and treatment of TGEV provided by this invention, the complex probiotics and the synergistic enzyme system form a synergistic effect. Lactic acid secreted by *Lactobacillus rhamnosus* can lower the intestinal pH, disrupting the TGEV survival environment; antimicrobial peptides produced by *Bacillus coagulans* can directly cleave the viral capsid protein; lysozyme can degrade viral envelope polysaccharides; and neutral protease, β-glucanase, and xylanase can destroy the viral adsorption sites on the intestinal mucosa. This quadruple action significantly enhances the antiviral effect.

This application discloses a method for preparing a fermented composition of honey, royal jelly, and propolis, the fermented composition itself, and its applications. The preparation method provided by this application includes: adding a first-stage substrate to a basic fermentation medium, inoculating with *Candida apis* and *Kluyveromyces martensii*, and carrying out a first-stage fermentation to obtain a first fermentation broth; adding a second-stage substrate to the first fermentation broth and carrying out a second-stage fermentation to obtain a second fermentation broth; and subjecting the second fermentation broth to cell disruption and post-treatment to obtain the fermented composition of honey, royal jelly, and propolis. This application uses *Candida apis* and *Kluyveromyces martensii* to carry out a two-stage synergistic fermentation of honey, royal jelly, and propolis as a triple substrate. After synergistic fermentation, cell disruption is performed to release yeast lysates. The resulting fermented composition is clear and transparent in appearance, has good stability, a rich rose aroma, and significantly inhibits inflammatory factors and promotes collagen synthesis.

This invention discloses a promoter-terminator combination for *Kluyveromyces martensii*, wherein the promoter... P STF2 The nucleotides are as shown in SEQ ID NO.1, and the terminator is... T STF2 The nucleotide sequence is shown in SEQ ID NO.2. The application of the *Kluyveromyces martensii* promoter-terminator combination in driving exogenous protein expression. This invention uses transcriptome sequencing technology to screen highly expressed genes from *Kluyveromyces martensii* transcriptome sequencing data using FPKM value as an indicator, obtaining an endogenous, highly efficient promoter-terminator combination. P STF2 ‑T STF2 .through egfp The reporter gene was validated and found to have stronger transcriptional activity, making it an ideal combination of regulatory elements for the efficient expression of exogenous proteins in *Kluyveromyces martensii*. Its application to the exogenous expression of myrosinase and xylanase resulted in efficient expression of both enzymes, demonstrating the good versatility and application value of this regulatory element combination.

The invention discloses a compound microbial functional fertilizer as well as a preparation method and application thereof, and belongs to the technical field of microbial fertilizers. The functional fertilizer is prepared by taking six functional strains including clostridium butyricum (CCTCC NO: M 2022151), bacillus coagulans (CGMCC NO.32216), kluyveromyces marxianus (CGMCC NO.24455) and the like as cores, matching with auxiliary materials including soy protein concentrate and the like and performing a two-stage compound fermentation process. Experiments show that the functional fertilizer can significantly increase the content of medium trace elements such as quick-acting phosphorus, potassium, calcium, magnesium, iron and zinc in soil, and effectively repair the degraded soil structure; when the fertilizer is used for wheat planting, the acre yield, the wheat protein content and the wet gluten content of wheat can be remarkably improved, and the crop quality is greatly improved. The soil remediation agent has the dual effects of soil remediation and crop yield increase and quality improvement, and a new technical scheme is provided for sustainable development of green agriculture.

The invention belongs to the field of microorganisms, and particularly relates to a kluyveromyces marxianus strain, a microbial agent and preparation and application of the kluyveromyces marxianus strain. The kluyveromyces marxianus strain comprises kluyveromyces marxianus AMCC 31690, the preservation number of the kluyveromyces marxianus AMCC 31690 is CCTCC NO: M 2024246, the preservation number of the kluyveromyces marxianus AMCC 31691 is CCTCC NO: M 2024247, and the preservation number of the kluyveromyces marxianus AMCC 30630 is CCTCC NO: M 20222107. The kluyveromyces marxianus provided by the invention has multiple tolerance of osmotic pressure resistance, acid resistance, cholate resistance and artificial stomach and intestinal juice resistance, and can survive and keep vitality in the artificial stomach and intestinal juice; meanwhile, the hydrophobic property and the self-agglutination capability are realized.

The invention belongs to the technical field of biology, and particularly relates to a cubilose compound rich in Kluyveromyces marxianus beta-glucan as well as a preparation method and application of the cubilose compound. According to the compound, kluyveromyces marxianus is used for fermenting or not fermenting a cubilose matrix, so that in-situ compounding and synergistic interaction of yeast-derived beta-glucan and cubilose-derived active ingredients (such as sialic acid and small molecule peptide) are realized. The preparation method comprises the following steps: cubilose pretreatment, yeast activation and inoculation, and post-treatment of a mixed product. Experiments prove that the compound has remarkable immune enhancement and skin health improvement effects, and can be used for preparing food, health care products, medicines or personal care products. The problems that a traditional cubilose product is low in absorption and utilization rate, single in function, uneven in physical mixing and the like are solved, and a novel efficient and high-valued cubilose deep processing technology is provided.

This invention discloses a Kluyveromyces martensii complex rich in organic selenium and high-yielding γ-aminobutyric acid (GABA) and its application in bird's nest dairy products, belonging to the field of biotechnology. The complex was obtained through ARTP mutagenesis screening to produce a high-GABA-producing Kluyveromyces martensii strain (GABA yield ≥2000 mg / kg). After selenium enrichment culture, the organic selenium content was ≥200 mg / kg and the organic selenium ratio was >99%. When this yeast complex was applied to the preparation of bird's nest milk, the synergistic effect of organic selenium, GABA, and bird's nest significantly improved the sleep rate in mice (90%), solving the problems of single function and low bioavailability in existing products. It is suitable for the field of health foods for sleep aid and immune regulation.

This invention belongs to the field of biomedical technology and discloses a method for efficiently and stably expressing canine parvovirus VP2 nanoparticles in *Kluyveromyces martensii*. The applicant prepared a double-replicant expression vector suitable for *Kluyveromyces martensii* through screening, assembly, and analysis. This vector achieved high conversion rate and high expression efficiency in *Kluyveromyces martensii*. The double-replicant expression vector pGKD32 is shown in SEQ ID NO.2. Using the *Kluyveromyces martensii* expression system provided by this invention, the applicant successfully expressed canine parvovirus VP2 in nanoparticle form, providing a new approach for the development of oral immunization strategies for canine parvovirus.

The invention discloses a method for screening kluyveromyces marxianus chassis cells and application of the method, and relates to the field of biochemistry, kluyveromyces marxianus from different sources is sequentially subjected to growth characteristic screening, genetic manipulation performance screening, extreme condition tolerance screening and gene editing efficiency screening, and a bacterial strain with the most comprehensive final performance is obtained. The screened kluyveromyces marxianus is kluyveromyces marxianus KM5, is preserved in the China Industrial Microbiological Culture Collection Center (CICC), has the serial number of CICC33373, and is applied to expression of lipase. According to the invention, a systematic high-throughput screening method is used to screen out a strain which can play a role in various application scenarios, the strain is used as a chassis cell, and fluorescent protein and lipase are successfully expressed according to a plasmid expression system constructed by the strain.

The present application relates to a kind of GDP-mannose dehydratase polypeptide-containing kloeckera gene modification cell, it can be applied to the synthesis of human milk oligosaccharide, belong to synthetic biology field.The technical scheme of the present application is a kind of gene modification cell, it includes the recombinant nucleic acid sequence of polypeptide with the activity of GDP-mannose dehydratase polypeptide (GDP-mannose dehydratase).The polypeptide with catalyzing GDP-mannose is converted into GDP-4-ketone-6-deoxymannose, it includes amino acid sequence with SEQ ID NO:1, SEQ ID NO:2 And / or with at least 70%, 75%, 80%, 85%, 90%, 95%, 99%, 100% identical amino acid sequence of polypeptide M1-M13.The present application provides a kind of kloeckera gene modification cell with the activity of synthesizing fucosyllactose.