Production of curing lactase

A production method and technology of lactase, applied in directions such as hydrolase fixed on/in organic carrier, can solve the problems of high price of low-lactose milk, influence of market sales, increase of production cost of liquid milk and milk powder, etc.

Active Publication Date: 2006-08-16
哈尔滨美华生物技术股份有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] However, the price of lactase used in the production of low-lactose milk is more expensive, which increases the production cost of liquid milk and milk powder, so the price of low-lactose milk is on the high side, and the market sales are affected to a certain extent. solve this problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] Take 5000mL of cultured and mature Kluyveromyces fragilis cell fermentation liquid, centrifuge at 10000r / min at high speed to obtain fresh and active bacterial cells, wash the bacterial cells with sterile water 1-2 times, and then use sterile water to prepare Cell suspension, the cell concentration is 5.0%-40.0%.

[0030] Take 500mL of bacterial suspension, add protease and β-glucanase, the dosage of protease is 50.0u / g wet bacteria, the dosage of β-glucanase is 20u / g wet bacteria, pH 6.5, keep warm at 42°C for 1.5 hours.

[0031] The enzyme-treated cell suspension is taken out, placed at -25°C for rapid freezing, and frozen completely, and then the frozen cells are rapidly thawed at 50°C to obtain a freeze-thawed cell suspension.

[0032] Then place the frozen-thawed cell suspension in a liquid shearing device and a high-pressure homogenizer for secondary wall breaking. The temperature at the inlet is maintained at about 20°C, the temperature at the outlet is not highe...

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PUM

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Abstract

The invention is about the production method for the immobilized lactase. It includes: (1) prepare the kluyveromyces Fragilis suspension, (2) breaking the cell wall by the enzyme, (3) breaking the cell wall by freeze-thaw, (4) breaking the cell wall by high-pressure homogenizer, (5) preparing the immobilized carrier liquid, (6) preparing the immobilized carrier liquid for lactase, (7) preparing the immobile liquid, (8) immobilization. So the low lactin milk is produced by the immobilized lactase, it can treat it continuously and the enzyme can't run off. So it can decrease the cost.

Description

(1) Technical field [0001] The invention relates to a production method of microbial preparations, in particular to a production method of microbial cell wall breaking and immobilized lactase. (2) Technical background [0002] The enzymes that can decompose lactose in the human small intestine include lactase (lactase) and β-galactosidase (β-galactosidase). Lactase is located in the brush border of the small intestinal mucosa, while β-galactosidase is located in the lysosome and cannot be decomposed. Lactose in the intestinal lumen. Lactase deficiency refers to the low activity of the membrane-bound enzyme lactase in the brush border chorionic cells of the small intestine. There are three types of lactase deficiency: (1) Congenital lactase deficiency: refers to the low or lack of lactase activity in the body since birth, which is caused by an autosomal recessive gene in the body. This type is rare. (2) Secondary lactase deficiency: refers to the temporary low lactase activ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04C12N11/08C12N9/24
Inventor 梁金钟
Owner 哈尔滨美华生物技术股份有限公司
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