Production of curing lactase
A production method and technology of lactase, applied in directions such as hydrolase fixed on/in organic carrier, can solve the problems of high price of low-lactose milk, influence of market sales, increase of production cost of liquid milk and milk powder, etc.
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[0029] Take 5000mL of cultured and mature Kluyveromyces fragilis cell fermentation liquid, centrifuge at 10000r / min at high speed to obtain fresh and active bacterial cells, wash the bacterial cells with sterile water 1-2 times, and then use sterile water to prepare Cell suspension, the cell concentration is 5.0%-40.0%.
[0030] Take 500mL of bacterial suspension, add protease and β-glucanase, the dosage of protease is 50.0u / g wet bacteria, the dosage of β-glucanase is 20u / g wet bacteria, pH 6.5, keep warm at 42°C for 1.5 hours.
[0031] The enzyme-treated cell suspension is taken out, placed at -25°C for rapid freezing, and frozen completely, and then the frozen cells are rapidly thawed at 50°C to obtain a freeze-thawed cell suspension.
[0032] Then place the frozen-thawed cell suspension in a liquid shearing device and a high-pressure homogenizer for secondary wall breaking. The temperature at the inlet is maintained at about 20°C, the temperature at the outlet is not highe...
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