55 results about "Small intestinal mucosa" patented technology

The invention discloses an application of enzymatic casein in promotion of porcine endocrine cell proliferation, belongs to the technical field of animal nutrition and feed, and provides an application of enzymatic casein in promotion of porcine endocrine cell proliferation, which does not aim at diagnosis and treatment of diseases. The invention provides a method for promoting pig endocrine cell proliferation by using casein enzymolysis. The method comprises the following steps: adaptively feeding pigs with daily ration with the crude protein content of 13% for 7 days; mixing the daily ration with the crude protein content of 13% with the enzymolysis casein according to the mass ratio of 95.5: 4.5 to obtain the daily ration containing the enzymolysis casein, and feeding twice a day for 28 days. According to the invention, a certain proportion of casein hydrolysate is added into the feed to regulate and control microbe composition of small intestinal mucosa of growing pigs, so that proliferation of small intestinal endocrine cells is further regulated, the secretion level of gastrointestinal hormone is increased, the gastrointestinal transport function of animals is finally enhanced, the absorption of nutrient substances is accelerated, and the growth performance of the pigs is improved.

The invention discloses a lactobacillus rhamnosus composition for relieving gastrointestinal diarrhea and application of the lactobacillus rhamnosus composition, the composition is composed of a probiotic mixture, a prebiotic mixture and a carrier, the probiotic mixture is composed of lactobacillus plantarum JY055 bacterial powder, lactobacillus rhamnosus JM039 bacterial powder and lactobacillus rhamnosus JY027 bacterial powder, and the prebiotic mixture is composed of lactobacillus plantarum JY055 bacterial powder, lactobacillus rhamnosus JM039 bacterial powder and lactobacillus rhamnosus JY027 bacterial powder. The prebiotic mixture comprises inulin, xylitol, lactulose, resistant dextrin, a hawthorn extract and a dandelion extract, and the carrier is modified montmorillonite. The composition for relieving gastrointestinal diarrhea provided by the invention is composed of a mixture of three probiotics and prebiotics, and the probiotics are adhered to small intestinal mucosa epithelial cells to form a mycoderm and form an intestinal barrier, so that colonization and growth of harmful bacteria are prevented, self-protection of intestinal epithelial cells is enhanced, and the damaged intestinal barrier is repaired; the prebiotic mixture provides a breeding place and nutrient elements for growth and breeding of the probiotics, and the efficacy can be improved through mutual cooperation of the prebiotic mixture and the probiotics.

The invention relates to the field of new biological materials, in particular to a high-packaging-dose antibacterial slow-release biological patch obtained by packaging at least two antibiotics with an acellular matrix material. The acellular matrix material is derived from any two of small intestinal submucosa, peritoneum, dermis, bladder basement membrane and pericardium; the antibiotics comprise hydrophobic antibiotics and hydrophilic antibiotics, and the hydrophobic antibiotics are selected from one or more of Beta-lactam antibiotics, rifamycin and macrolide antibiotics; the hydrophilic antibiotics are tetracycline antibiotics. The coating-free drug-loaded sustained-release system based on a lamination process kills bacteria in a short period, prevents colonization and reproduction of bacteria in a wound / patch, realizes synergistic and continuous release of antibiotics along with a wound healing process, guides tissue regeneration and synchronously realizes degradation, is applied to a high-incidence infection period of a surgical operation, reduces adhesion, and has a good application prospect. The abdominal wall strength is enhanced, and the growth of wound granulation tissues is promoted.

The invention discloses a production device for extracting sodium heparin by utilizing pig small intestinal mucosa, comprising a feeding roller, a sleeve rod and a base, wherein the sleeve rod comprises an upper sleeve rod and a lower sleeve rod connected to the lower part of the upper sleeve rod, a deep hole 1 cooperating with the upper sleeve rod is provided on the upper part of the base, a deep hole 2 cooperating with the lower sleeve rod is provided at the bottom of the deep hole 1, the deep hole 2 passes through the lower side surface of the base, the upper sleeve rod and the lower sleeve rod are respectively located in the deep hole 1 and the deep hole 2, and an annular scraper is arranged on the outer wall of the upper sleeve rod. The invention can scrape off the mucosa in a long section of the small intestine, and at the same time does not affect the subsequent production of the small intestine as casing during the processing.

Disclosed are a decellularized matrix material, a preparation method therefor and use thereof. The preparation method includes: subjecting small intestinal submucosa to pre-treatment, and subjecting the small intestinal submucosa after the pre-treatment to swelling treatment, freeze-thaw treatment, oxidase immersion treatment, and surfactant treatment in sequence, so as to obtain the decellularized matrix material. In the present application, the combination of physical swelling, physical freezing and thawing, and chemical elution guarantees a high decellularization efficiency; moreover, by adding the oxidase at an appropriate time during the decellularization treatment process, the oxidase thoroughly permeates into the decellularized matrix, and thereby plays a buffering role in the tissue when the surfactant is applied, ensuring the intactness of the collagen structure in the extracellular matrix.

The invention relates to the technical field of heparin sodium purification, and discloses an efficient extraction method for reducing the content of impurity residues in heparin sodium, which comprises the following preparation steps: S1, mixing small intestine mucosa with deionized water, adding alkaline protease, filtering after enzymolysis is completed, and collecting filtrate; s2, modified adsorption resin is added, alkaline protease is added again after stirring adsorption is completed, modified adsorption resin is added again after enzymolysis is completed, elution is conducted after filtering and washing are conducted, and an eluent and a washing solution are combined to obtain a mixed solution; and S3, adding an ethanol water solution, filtering and collecting heparin sodium precipitate, and purifying and drying to obtain the heparin sodium. According to the method, secondary enzymolysis and process adsorption are adopted, so that the operation time can be effectively shortened, the adsorption efficiency is improved, non-enzymolysis impurities doped in the adsorption process can be effectively avoided, resin adopted for adsorption is modified, the service life of the resin is prolonged, the use efficiency of the resin is improved, and the resin can still keep good adsorption capacity at high temperature.

The present invention belongs to the field of food and medicine, and specifically relates to a small intestinal mucosa extract having a preventive and therapeutic effect on depression. The present invention also provides a preparation method of the small intestinal mucosa extract and a health product, food or medicine containing the small intestinal mucosa extract. The small intestinal mucosa extract provided by the present invention has been found through pharmacological experiments on animal models to significantly improve the depression and anxiety state of depression model rats and increase the body weight of rats, and the therapeutic effect on depression is significantly better than the prior art.

This invention belongs to the field of food nutrition and functional factors, specifically relating to a method for preparing highly immunomodulatory ovotransferrin (OVT) hydrolysates based on an adult gastrointestinal simulated digestive system. The method involves processing OVT using a simulated adult gastrointestinal tract to prepare OVT hydrolysates containing HTEGSTT, TTSY, and / or RTAGWVIPMG. This invention reveals that OVT exhibits enhanced immunomodulatory activity after gastrointestinal digestion and hydrolysis, and is more readily hydrolyzed in simulated adult digestive fluids than in infant digestive fluids. Comparative experiments show that nutritional intervention with OVT simulated digestive products from adults and infants can promote the production of short-chain fatty acids such as acetic acid, propionic acid, and butyric acid, effectively alleviate swelling and damage to the small intestinal mucosa caused by inflammation, significantly improve intestinal barrier immune function, and OVT IA exhibits higher intestinal immune activity than OVT IB.

The application provides a functionalized Janus extracellular matrix hydrogel scaffold, a preparation method and application thereof. The scaffold comprises a decellularized duck small intestinal mucosa layer which is co-functionalized by a polymeric flavonoid substance with a benzopyran ring as a basic structural unit and trivalent gallium ions, and a hydrogel layer with in-situ gelation and self-repairing properties formed on one side of the mucosa layer. In use, the functionalized side without gel is oriented towards the repaired tendon, and the side with gel is oriented towards the surrounding soft tissue, so as to form an inner tissue integration interface and an outer anti-adhesion barrier interface. The scaffold retains the collagen fiber structure of the decellularized matrix, has antioxidant, microenvironment-responsive release, antibacterial and good blood compatibility, can reduce peritendinous fibrous adhesion in an animal model of tendon rupture, improve tendon sliding and improve the mechanical strength of the repaired part.

The present invention relates to a biocomposite, regenerative, high-strength ligament graft, the ligament graft being woven from at least four types of ligament fiber bundles; the ligament fiber bundles being woven from different proportions of porcine small intestinal submucosal fibers and composite fibers; wherein the porcine small intestinal submucosal fibers are made by curling the porcine small intestinal submucosal layer; and the composite fibers are made by wrapping the porcine small intestinal submucosal layer around the surface of the high-strength fibers. The ligament graft of the present invention is woven from porcine small intestinal submucosal fibers and porcine small intestinal submucosal composite high-strength fibers. The porcine small intestinal submucosal layer retains extracellular matrix components and microstructures, facilitating cell migration, adhesion, and proliferation. The composite high-strength fibers are prepared by combining the composite high-strength fibers with a degradable polymer material, which serves as a mechanical support component. The complementary advantages of the degradable polymer material give the ligament graft of the present invention both good biocompatibility and strength.

The invention discloses a system for automatically counting small intestine mucosa, and relates to the technical field of small intestine processing, the system for automatically counting small intestine mucosa comprises: a conveying device configured to convey small intestine; the counting device is configured to count the small intestines to obtain an initial small intestine number; the control device is connected with the counting device, the control device is configured to correct the initial small intestine number based on a correction model to obtain a target small intestine number, and when the target small intestine number is equal to a small intestine number threshold value, a valve of the enzymolysis device is controlled to be opened so that the small intestine mucosa can be conveyed to the enzymolysis device. The system for automatically counting the small intestine mucosa can accurately record the processing quantity of small intestines so as to ensure the uniformity of enzymolysis batches of the small intestine mucosa, so that the titer and yield stability of heparin sodium are improved.

The application discloses a new technology for extracting crude heparin sodium by low-salt composite enzyme two-step incubation enzymolysis method, and belongs to the technical field of production process of extracting crude heparin sodium from pig small intestinal mucosa fluid. In the application, the composite enzyme enzymolysis technology is used under low-salt condition, so that the protein molecules connected with heparin sodium macromolecules are cut at multiple sites and saturated, thereby effectively releasing free heparin sodium macromolecules with anticoagulant biological activity; the potency of heparin sodium in the obtained enzymolysis liquid is 14.5 USPU / ML, which is equivalent to a yield of 116,000 USPU / root; and the yield of the obtained solid crude heparin sodium is 98,000 American standard units / root. Compared with the existing production technology, the total amount of wastewater discharge is reduced by 20%, and the total salt content in the wastewater is reduced by 78%. The application meets the needs of industrial production in two aspects of improving the yield of crude heparin sodium product and greatly reducing the content of wastewater pollutants.

The invention discloses an anti-tumor active component separated from sheep small intestine mucosa and a method and application thereof, and the method specifically comprises the following steps: cleaning waste sheep small intestine after animal slaughtering with normal saline, removing connective tissue and intestinal contents, scraping off the mucosa, pre-freezing the pretreated sheep small intestine mucosa, and freeze-drying the pre-frozen sheep small intestine mucosa to obtain the anti-tumor active component separated from the sheep small intestine mucosa. And grinding the freeze-dried product in a mortar to obtain sheep small intestine mucosa powder. Adding a phosphate buffer solution into the pretreated sheep small intestine mucosa powder in proportion, and performing low-temperature stirring extraction for 2-4 hours; and filtering the extracting solution with gauze or a screen, centrifuging, taking the supernatant, and freeze-drying to obtain the crude extract of the small intestinal mucosa of the sheep, wherein the yield is 36.11%. The crude extract is re-dissolved with deionized water and centrifuged, gel chromatography is used for desalination, purification is carried out, the component SI-2 with the antitumor activity is obtained, the half inhibition concentration (IC50) of the component to human colon cancer cells 29 (HT-29) is 0.786 + / -0.051 mg / mL, and the half inhibition concentration (IC50) of the component to human colon cancer cells 116 (HCT-116) is 0.095 + / -0.041 mg / mL.

The application discloses a salt-free production technology of heparin sodium crude product extracted by enzyme hydrolysis method of biological enzyme fermentation, and belongs to the technical field of production of heparin sodium crude product extracted from pig small intestinal mucosa. The application adopts controllable biological fermentation technology of bioactive enzyme combined with enzyme hydrolysis method to extract heparin sodium biological macromolecule with anticoagulant biological activity. The yield of anticoagulant activity in the obtained enzyme hydrolysis liquid is 96,000 USPU / root, and the yield of dried solid heparin sodium crude product is 82,000 US standard units / root. The technical scheme of the application greatly improves the yield of heparin sodium crude product, simultaneously reduces 73% of wastewater and 99% of chloride ion content, greatly reduces the difficulty of wastewater treatment, makes it possible to change waste into treasure by comprehensive recycling of wastewater, and is favorable for industrial application.

The application provides a method for producing heparin sodium through microbial fermentation and belongs to the technical field of polysaccharide preparation and is subordinated to IPC classification number C08B37.The method comprises the following steps: crushing pig small intestinal mucosa, high-pressure treatment, ultrasonic treatment, inoculating composite microorganisms for anaerobic fermentation, adsorbing the fermentation liquor through resin, eluting, alcohol precipitation, collecting the precipitate and obtaining heparin sodium.The pig small intestinal mucosa is crushed, high-pressure treated and ultrasonically treated, the dissolution of heparin sodium and nutrient components is promoted, the binding force between heparin sodium and other impurities is weakened, the composite microorganisms can not only decompose polysaccharide-protein compound and intercellular substance components in the mucosa but also degrade protein, fat and other impurity components in the mucosa, and the interference of the impurities on the extraction process of heparin sodium is reduced, and the resin can enrich heparin sodium and realize the separation of heparin sodium and the fermentation liquor.The various steps have a synergistic effect, and the titer and yield of heparin sodium are improved.

The invention discloses a cryogel chromatographic separation method of heat shock protein 70, which comprises the following specific steps: taking cryogel with a tertiary amino anion exchange functional group as a separation medium, and flushing the cryogel medium with a phosphate buffer solution to realize balance; then, adjusting the pH value of a heat shock protein 70 crude protein solution obtained by small intestine mucosa treatment to 5.8-6.5, and loading a sample at a flow rate of 1 cm / min; after sample loading is finished, flushing the cryogel medium by using a phosphate buffer solution with the same concentration so as to remove unadsorbed proteins and other impurities; flushing the cryogel medium by using a phosphate buffer solution containing 0.05-1 M of NaCl to carry out gradient elution, and collecting elution components under different salt concentrations in sections; and finally, carrying out regeneration treatment on the cryogel medium by using a phosphate buffer solution containing 2M NaCl and deionized water in sequence, so as to realize reutilization. The method has the characteristics of simplicity and convenience in operation, controllable cost, high product purity, ideal recovery rate and the like, and an efficient and reliable technical scheme is provided for separation of the heat shock protein 70.

Disclosed are a decellularized matrix material, a preparation method therefor and use thereof. The preparation method includes: subjecting small intestinal submucosa to pre-treatment, and subjecting the small intestinal submucosa after the pre-treatment to swelling treatment, freeze-thaw treatment, oxidase immersion treatment, and surfactant treatment in sequence, so as to obtain the decellularized matrix material. In the present application, the combination of physical swelling, physical freezing and thawing, and chemical elution guarantees a high decellularization efficiency; moreover, by adding the oxidase at an appropriate time during the decellularization treatment process, the oxidase thoroughly permeates into the decellularized matrix, and thereby plays a buffering role in the tissue when the surfactant is applied, ensuring the intactness of the collagen structure in the extracellular matrix.

The application belongs to the technical field of heparin extraction, and particularly relates to a method for determining the abundance of high anti-IIa activity heparin of pig small intestinal mucosa. The method comprises the following steps: (1) crushing fresh pig small intestinal mucosa; (2) using a PowerSoil kit to lyse the mucosa cells crushed in step (1), and performing water bath at 50-65 DEG C for 10-20 min, and then adding an elution buffer to elute the DNA; (3) using PCR to amplify the eluted DNA solution; (4) using a Nextera XT DNA library preparation kit to construct a library, and then using an Illumina NovaSeq 6000 sequencer to perform high-throughput sequencing; (5) using a DADA2 process to denoise and remove chimeric sequences, and using a 97% similarity threshold to cluster to generate OTU, and using a Greengenes database to annotate; (6) then using an LEfSe algorithm to screen differential flora, and using a random forest model to predict heparin activity.

The invention discloses an application of protein zinc with medium chelating strength in improvement of heat stress resistance of chicken, and provides a new function of protein zinc with medium chelating strength, and zinc is added under a heat stress condition to relieve small intestine mucosa injury of heat stress broiler chicken, the medium-chelating-strength protein zinc is added into the feed, so that the relieving effect on the heat-stress small intestinal mucosal injury of the broiler chicken is obvious; wherein the 60mg Zn / kg feed obtained by supplementing the protein zinc with medium chelating strength has a more obvious effect of relieving the heat stress small intestine mucosa injury of the broiler chicken; specifically, the permeability of each section of the small intestine of the broiler chicken is reduced, and the barrier function is enhanced. Therefore, the supplement of the protein zinc with medium chelating strength in the feed is most beneficial to the improvement of the small intestine mucosa injury of the heat stress broiler chicken. The protein zinc with medium chelating strength can be effectively used for preparing a feed additive for improving the heat stress resistance of chicken and preparing a feed additive for improving the small intestine mucosa injury of heat stress chicken.

This invention discloses a method for preparing a water-soluble homogeneous heteropolysaccharide from highland barley and its novel application in promoting the expression of β-galactosidase in the small intestinal mucosa, belonging to the field of polysaccharide preparation and application technology. This invention provides a method for preparing the water-soluble homogeneous heteropolysaccharide HBP-1a from highland barley, involving extraction, alcohol precipitation, decolorization, and deproteinization of highland barley raw materials, followed by separation and purification using anion exchange column chromatography and gel column chromatography. The water-soluble homogeneous heteropolysaccharide HBP-1a was successfully isolated from the highland barley polysaccharide. This invention is the first to discover that the water-soluble homogeneous heteropolysaccharide HBP-1a can effectively promote the expression of β-galactosidase in the small intestinal mucosa and can be used to improve symptoms related to lactose intolerance diarrhea. This invention explores a new use for the water-soluble homogeneous heteropolysaccharide HBP-1a from highland barley, broadening its application fields and possessing broad prospects and market value.

This invention discloses a composite scaffold for bone repair and its preparation method. The composite scaffold for bone repair consists of a porous polylactic acid (PLA) scaffold in the middle and an outer layer of small intestinal submucosal matrix sponge. The ratio of the diameter of the composite scaffold to the diameter of the PLA porous scaffold is 2-4. The preparation method includes the following steps: S1: preparing small intestinal submucosal matrix; S2: preparing a small intestinal submucosal matrix solution; S3: preparing a PLA porous scaffold; S4: preparing a composite scaffold of small intestinal submucosal matrix sponge and PLA porous scaffold; S5: cross-linking treatment, placing the composite scaffold of small intestinal submucosal matrix sponge and PLA porous scaffold in a cross-linking treatment solution, and finally freeze-drying to obtain the composite scaffold for bone repair. The composite scaffold for bone repair prepared by this invention has good mechanical properties and will be gradually degraded and replaced and absorbed by newly formed bone tissue.

The present invention discloses a bio-fermentation and enzymatic hydrolysis production technology for extracting crude sodium heparin from the mucosa of the pig small intestine, belonging to the technical field of production for extracting crude sodium heparin. The present invention uses a bio-active enzyme controlled bio-fermentation technology combined with an enzymatic hydrolysis method to extract heparin sodium biomacromolecules with anticoagulant biological activity. The anticoagulant activity yield in the resulting enzymatic hydrolyzate is 114,000 USPU / root, and the yield of the dried solid crude sodium heparin obtained is 88,843 USPU / root, or 1,126 roots / 100 million units. Compared with the process of enzymatic hydrolysis alone without the combined bio-active enzyme bio-fermentation technology, the yield is increased by 22.2%. This demonstrates that the bio-active enzyme controlled bio-fermentation technology combined with the enzymatic hydrolysis extraction technology has significant advantages in industrial production. At the same time, the salt usage in production is 1.2%, which is a 66% decrease compared to the common salt usage in existing industry methods, reducing the difficulty of wastewater treatment and facilitating industrial application.

The invention provides composite hydrogel and a preparation method and application thereof, and belongs to the technical field of biomedical materials, the composite hydrogel provided by the invention comprises melatonin carbon dots and porcine small intestinal submucosa hydrogel, the melatonin carbon dots are dissolved in the porcine small intestinal submucosa hydrogel, the particle size of the melatonin carbon dots is 1.5-2.5 nm, and the melatonin carbon dots are dissolved in the porcine small intestinal submucosa hydrogel. The loading capacity of the melatonin carbon dots in the composite hydrogel is 150 to 450 [mu] g / mL. The composite hydrogel provided by the invention can effectively inhibit the inflammatory response of achilles tendinopathy, can promote tendon regeneration, and has the advantages of minimally invasive effect, high drug curative effect and small side effect when being used for treating achilles tendinopathy.

The invention discloses an artificial blood vessel basal layer and a preparation method thereof and relates to the technical field of artificial blood vessel preparation. The preparation method of the artificial blood vessel basal layer comprises the following steps: providing a small intestine submucosa subjected to decellularization treatment; the small intestinal submucosa is wound on the surface of a long-strip-shaped base body with the cylindrical outer surface, overlapping areas of the small intestinal submucosa are attached, and then a clamp is used for clamping to obtain a pretreatment piece; the pretreated part is dried and then subjected to heat treatment under the condition that the temperature ranges from 80 DEG C to 150 DEG C, and the overlapped areas of the small intestine submucosa are connected. The small intestine submucosa can be firmly connected, and the problem that embolism is likely to be caused when a micro suture line is adopted for suturing can be solved.

The invention discloses modified bi-crosslinking temperature-sensitive extracellular matrix hydrogel and a preparation method thereof, and relates to the technical field of adhesives for colorectal anastomosis, and the preparation method comprises the following steps: (1) preparing SIS fragments: selecting intestinal segments, cutting the intestinal segments into pieces, and disinfecting the intestinal segments; (2) carrying out decellularization and crushing treatment on the SIS fragments; (3) preparing an SIS hydrogel; and (4) preparing the BA / SIS-SC hydrogel. The multifunctional temperature-sensitive extracellular matrix hydrogel is prepared by taking boric acid modified porcine small intestinal submucosa extracellular matrix and four-arm polyethylene glycol succinimide carbonate as raw materials, and a series of experiments show that the prepared hydrogel is stable in structure and high in temperature sensitivity, and can be used for preparing the temperature-sensitive extracellular matrix hydrogel. The injectable plugging agent has good tissue adhesion and mechanical property and excellent blasting resistance and self-healing property, and can effectively plug an anastomotic stoma in combination with the injectable property of the injectable plugging agent. In-vitro experiments prove that the hydrogel has good antibacterial property and excellent cell and blood compatibility. The research provides a direction for developing a novel multifunctional material for preventing and treating anastomotic leakage.

The subject invention provides therapeutic compositions, and uses thereof for the treatment or amelioration of injury to small intestine mucosa. In preferred embodiments, the composition comprises one or more nutrients and / or electrolytes that acquire or retain considerable absorptive capacity.

The invention discloses application of siRNA in a postprandial hyperglycemia treatment preparation, and belongs to the technical field of biology. A siRNA sequence for silencing maltase-glucoamylase protein expression is designed by analyzing mRNA sequences of human and mouse maltase-glucoamylase, and unmodified siRNA and 2 '-OMe, 2'-F basic group modified siRNA are respectively synthesized. The two kinds of siRNA transfect human small intestinal mucosal epithelial cells through lipidosome, the silence efficiency of the unmodified siRNA on the MGAM gene is 81.54%, and the silence efficiency of the base modified siRNA on the MGAM gene is 82.09%. When the two siRNAs are intravenously injected into a type 2 diabetes mellitus model mouse, the expression of maltase-glucoamylase can be effectively silenced, postprandial hyperglycemia is reduced, the stability of the base modified siRNA is improved, the drug effect time can be prolonged, and the medication frequency can be reduced.

The present invention discloses a SIEC-S cell suitable for serum-free full suspension and a culture domestication method, belonging to the technical field of SIEC-S cell culture. The SIEC-S cell was deposited with the General Microbiology Center of the China Microbial Culture Collection Management Committee on December 25, 2022, with the deposit number CGMCC No. 45338, and the deposit address is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. It is classified and named as porcine small intestinal mucosal epithelial suspension cell. The SIEC-S cell directly grows in a serum-free CD medium, reducing the usage amount of serum and lowering the cost. The SIEC-S can be suspended and cultured on a large scale in a bioreactor, improving the yield of downstream vaccine antigen content.

This utility model discloses a precision filtration device for heparin sodium extract, relating to the technical field of filtration components. It includes a filter box with an oil-floating filter plate installed inside; a meat grinding box for breaking down the small intestinal mucosa; a heating and stirring box located to the side of the meat grinding box; and a settling cylinder located to the side of the heating and stirring box. The settling cylinder is a semi-cylindrical shape with an open top, and a mounting groove is provided at one end of the top of the settling cylinder. An outwardly extending curved spout is welded to the mounting groove. The filter box is located below the settling cylinder. A supporting arc seat is provided at the bottom of the settling cylinder, and the settling cylinder is rotatably connected to the supporting arc seat. A rotating shaft is fixed at the center of the end face of the settling cylinder, and a geared motor is connected to the other end of the rotating shaft. This device integrates multiple processes such as breaking down the small intestinal mucosa, heating and enzymatic hydrolysis, settling separation, and filtration into one unit.

The invention relates to the technical field of heparin sodium, in particular to a heparin sodium efficient extraction process based on an enzymolysis method. The method comprises the steps of raw material treatment, heating enzymolysis, cooling adsorption, elution, ethanol precipitation, dehydration drying and index determination. In the high-efficiency extraction process of the heparin sodium based on the enzymolysis method, the small intestine mucous membrane is crushed into a minced state to increase the enzyme contact area, and a low-temperature graded heating enzymolysis process is combined, so that the high-efficiency decomposition of a heparin-protein compound is realized, and the problems that the enzymolysis is not thorough and the temperature needs to be increased for multiple times in the traditional enzymolysis method are effectively solved; meanwhile, insoluble impurities are removed through filtration while the solution is hot, protein residues and impurity interference are reduced in cooperation with cooled resin adsorption and gradient elution, the yield of the heparin sodium is increased, production energy consumption and purification difficulty are reduced, and therefore the overall efficiency and economical efficiency of extraction of the heparin sodium are improved.