16 results about "Small intestinal mucosa" patented technology

This invention belongs to the field of food nutrition and functional factors, specifically relating to a method for preparing highly immunomodulatory ovotransferrin (OVT) hydrolysates based on an adult gastrointestinal simulated digestive system. The method involves processing OVT using a simulated adult gastrointestinal tract to prepare OVT hydrolysates containing HTEGSTT, TTSY, and / or RTAGWVIPMG. This invention reveals that OVT exhibits enhanced immunomodulatory activity after gastrointestinal digestion and hydrolysis, and is more readily hydrolyzed in simulated adult digestive fluids than in infant digestive fluids. Comparative experiments show that nutritional intervention with OVT simulated digestive products from adults and infants can promote the production of short-chain fatty acids such as acetic acid, propionic acid, and butyric acid, effectively alleviate swelling and damage to the small intestinal mucosa caused by inflammation, significantly improve intestinal barrier immune function, and OVT IA exhibits higher intestinal immune activity than OVT IB.

The invention discloses a system for automatically counting small intestine mucosa, and relates to the technical field of small intestine processing, the system for automatically counting small intestine mucosa comprises: a conveying device configured to convey small intestine; the counting device is configured to count the small intestines to obtain an initial small intestine number; the control device is connected with the counting device, the control device is configured to correct the initial small intestine number based on a correction model to obtain a target small intestine number, and when the target small intestine number is equal to a small intestine number threshold value, a valve of the enzymolysis device is controlled to be opened so that the small intestine mucosa can be conveyed to the enzymolysis device. The system for automatically counting the small intestine mucosa can accurately record the processing quantity of small intestines so as to ensure the uniformity of enzymolysis batches of the small intestine mucosa, so that the titer and yield stability of heparin sodium are improved.

The application discloses a new technology for extracting crude heparin sodium by low-salt composite enzyme two-step incubation enzymolysis method, and belongs to the technical field of production process of extracting crude heparin sodium from pig small intestinal mucosa fluid. In the application, the composite enzyme enzymolysis technology is used under low-salt condition, so that the protein molecules connected with heparin sodium macromolecules are cut at multiple sites and saturated, thereby effectively releasing free heparin sodium macromolecules with anticoagulant biological activity; the potency of heparin sodium in the obtained enzymolysis liquid is 14.5 USPU / ML, which is equivalent to a yield of 116,000 USPU / root; and the yield of the obtained solid crude heparin sodium is 98,000 American standard units / root. Compared with the existing production technology, the total amount of wastewater discharge is reduced by 20%, and the total salt content in the wastewater is reduced by 78%. The application meets the needs of industrial production in two aspects of improving the yield of crude heparin sodium product and greatly reducing the content of wastewater pollutants.

The application discloses a salt-free production technology of heparin sodium crude product extracted by enzyme hydrolysis method of biological enzyme fermentation, and belongs to the technical field of production of heparin sodium crude product extracted from pig small intestinal mucosa. The application adopts controllable biological fermentation technology of bioactive enzyme combined with enzyme hydrolysis method to extract heparin sodium biological macromolecule with anticoagulant biological activity. The yield of anticoagulant activity in the obtained enzyme hydrolysis liquid is 96,000 USPU / root, and the yield of dried solid heparin sodium crude product is 82,000 US standard units / root. The technical scheme of the application greatly improves the yield of heparin sodium crude product, simultaneously reduces 73% of wastewater and 99% of chloride ion content, greatly reduces the difficulty of wastewater treatment, makes it possible to change waste into treasure by comprehensive recycling of wastewater, and is favorable for industrial application.

The application provides a method for producing heparin sodium through microbial fermentation and belongs to the technical field of polysaccharide preparation and is subordinated to IPC classification number C08B37.The method comprises the following steps: crushing pig small intestinal mucosa, high-pressure treatment, ultrasonic treatment, inoculating composite microorganisms for anaerobic fermentation, adsorbing the fermentation liquor through resin, eluting, alcohol precipitation, collecting the precipitate and obtaining heparin sodium.The pig small intestinal mucosa is crushed, high-pressure treated and ultrasonically treated, the dissolution of heparin sodium and nutrient components is promoted, the binding force between heparin sodium and other impurities is weakened, the composite microorganisms can not only decompose polysaccharide-protein compound and intercellular substance components in the mucosa but also degrade protein, fat and other impurity components in the mucosa, and the interference of the impurities on the extraction process of heparin sodium is reduced, and the resin can enrich heparin sodium and realize the separation of heparin sodium and the fermentation liquor.The various steps have a synergistic effect, and the titer and yield of heparin sodium are improved.

The invention discloses a cryogel chromatographic separation method of heat shock protein 70, which comprises the following specific steps: taking cryogel with a tertiary amino anion exchange functional group as a separation medium, and flushing the cryogel medium with a phosphate buffer solution to realize balance; then, adjusting the pH value of a heat shock protein 70 crude protein solution obtained by small intestine mucosa treatment to 5.8-6.5, and loading a sample at a flow rate of 1 cm / min; after sample loading is finished, flushing the cryogel medium by using a phosphate buffer solution with the same concentration so as to remove unadsorbed proteins and other impurities; flushing the cryogel medium by using a phosphate buffer solution containing 0.05-1 M of NaCl to carry out gradient elution, and collecting elution components under different salt concentrations in sections; and finally, carrying out regeneration treatment on the cryogel medium by using a phosphate buffer solution containing 2M NaCl and deionized water in sequence, so as to realize reutilization. The method has the characteristics of simplicity and convenience in operation, controllable cost, high product purity, ideal recovery rate and the like, and an efficient and reliable technical scheme is provided for separation of the heat shock protein 70.

The application belongs to the technical field of heparin extraction, and particularly relates to a method for determining the abundance of high anti-IIa activity heparin of pig small intestinal mucosa. The method comprises the following steps: (1) crushing fresh pig small intestinal mucosa; (2) using a PowerSoil kit to lyse the mucosa cells crushed in step (1), and performing water bath at 50-65 DEG C for 10-20 min, and then adding an elution buffer to elute the DNA; (3) using PCR to amplify the eluted DNA solution; (4) using a Nextera XT DNA library preparation kit to construct a library, and then using an Illumina NovaSeq 6000 sequencer to perform high-throughput sequencing; (5) using a DADA2 process to denoise and remove chimeric sequences, and using a 97% similarity threshold to cluster to generate OTU, and using a Greengenes database to annotate; (6) then using an LEfSe algorithm to screen differential flora, and using a random forest model to predict heparin activity.

This invention discloses a method for preparing a water-soluble homogeneous heteropolysaccharide from highland barley and its novel application in promoting the expression of β-galactosidase in the small intestinal mucosa, belonging to the field of polysaccharide preparation and application technology. This invention provides a method for preparing the water-soluble homogeneous heteropolysaccharide HBP-1a from highland barley, involving extraction, alcohol precipitation, decolorization, and deproteinization of highland barley raw materials, followed by separation and purification using anion exchange column chromatography and gel column chromatography. The water-soluble homogeneous heteropolysaccharide HBP-1a was successfully isolated from the highland barley polysaccharide. This invention is the first to discover that the water-soluble homogeneous heteropolysaccharide HBP-1a can effectively promote the expression of β-galactosidase in the small intestinal mucosa and can be used to improve symptoms related to lactose intolerance diarrhea. This invention explores a new use for the water-soluble homogeneous heteropolysaccharide HBP-1a from highland barley, broadening its application fields and possessing broad prospects and market value.

This invention discloses a composite scaffold for bone repair and its preparation method. The composite scaffold for bone repair consists of a porous polylactic acid (PLA) scaffold in the middle and an outer layer of small intestinal submucosal matrix sponge. The ratio of the diameter of the composite scaffold to the diameter of the PLA porous scaffold is 2-4. The preparation method includes the following steps: S1: preparing small intestinal submucosal matrix; S2: preparing a small intestinal submucosal matrix solution; S3: preparing a PLA porous scaffold; S4: preparing a composite scaffold of small intestinal submucosal matrix sponge and PLA porous scaffold; S5: cross-linking treatment, placing the composite scaffold of small intestinal submucosal matrix sponge and PLA porous scaffold in a cross-linking treatment solution, and finally freeze-drying to obtain the composite scaffold for bone repair. The composite scaffold for bone repair prepared by this invention has good mechanical properties and will be gradually degraded and replaced and absorbed by newly formed bone tissue.

This utility model discloses a precision filtration device for heparin sodium extract, relating to the technical field of filtration components. It includes a filter box with an oil-floating filter plate installed inside; a meat grinding box for breaking down the small intestinal mucosa; a heating and stirring box located to the side of the meat grinding box; and a settling cylinder located to the side of the heating and stirring box. The settling cylinder is a semi-cylindrical shape with an open top, and a mounting groove is provided at one end of the top of the settling cylinder. An outwardly extending curved spout is welded to the mounting groove. The filter box is located below the settling cylinder. A supporting arc seat is provided at the bottom of the settling cylinder, and the settling cylinder is rotatably connected to the supporting arc seat. A rotating shaft is fixed at the center of the end face of the settling cylinder, and a geared motor is connected to the other end of the rotating shaft. This device integrates multiple processes such as breaking down the small intestinal mucosa, heating and enzymatic hydrolysis, settling separation, and filtration into one unit.

The invention relates to the technical field of heparin sodium, in particular to a heparin sodium efficient extraction process based on an enzymolysis method. The method comprises the steps of raw material treatment, heating enzymolysis, cooling adsorption, elution, ethanol precipitation, dehydration drying and index determination. In the high-efficiency extraction process of the heparin sodium based on the enzymolysis method, the small intestine mucous membrane is crushed into a minced state to increase the enzyme contact area, and a low-temperature graded heating enzymolysis process is combined, so that the high-efficiency decomposition of a heparin-protein compound is realized, and the problems that the enzymolysis is not thorough and the temperature needs to be increased for multiple times in the traditional enzymolysis method are effectively solved; meanwhile, insoluble impurities are removed through filtration while the solution is hot, protein residues and impurity interference are reduced in cooperation with cooled resin adsorption and gradient elution, the yield of the heparin sodium is increased, production energy consumption and purification difficulty are reduced, and therefore the overall efficiency and economical efficiency of extraction of the heparin sodium are improved.

The utility model discloses a device for preparing crude heparin sodium from small intestinal mucosa, which comprises an integrated frame, a PLC (programmable logic controller) is mounted on one side of the integrated frame, and a primary enzymolysis tank and a secondary enzymolysis tank are respectively fixed at two ends inside the integrated frame. When the device runs, treated small intestine mucous membrane slurry is added into the primary enzymolysis tank through the corrosion-resistant pump on the feeding pipe fitting, and the external temperature control heating sleeves are fixed on the outer walls of the primary enzymolysis tank and the secondary enzymolysis tank; a water bath heating cavity which is provided with an electric heating wire and a temperature sensor and is arranged in the side wall of the external temperature control heating sleeve is matched, the temperature control heating effect on the primary enzymolysis tank and the secondary enzymolysis tank can be achieved, and then the timing control effect of the PLC is matched, so that after the temperature control timing reaction of the materials in the primary enzymolysis tank is achieved, the reaction time is shortened; and the material is automatically transferred into the secondary enzymolysis tank through a corrosion-resistant pump on the material transferring pipe fitting to complete secondary temperature control timing reaction, so that the device realizes continuous two-time enzymolysis treatment.

The invention discloses heparin sodium extraction equipment, and belongs to the technical field of heparin sodium production, the heparin sodium extraction equipment comprises a machine body, the inner side and the outer side of the machine body are jointly provided with a detection component, the detection component comprises a connecting frame jointly and fixedly connected to the upper sides of sliding blocks located on the front side and the rear side, and the left side of the lower end of the connecting frame is fixedly connected with a first fixing plate; the left side and the right side of the first fixing plate are jointly provided with through holes in a transverse linear array in a penetrating mode, the upper sides of the interiors of the through holes are provided with limiting grooves, the interiors of the limiting grooves are slidably connected with limiting blocks, and the lower sides of the limiting blocks are fixedly connected with scrapers. In the process of scraping the small intestine mucosa by the scraper, the optical current transformer scans the state of the small intestine muscle layer in real time, and if the small intestine muscle layer is damaged, the position of the scraper can be adjusted in time, so that the influence of I / III type collagen released after the muscle layer is damaged on the quality of the mucosa is effectively reduced.

The present application relates to heparin sodium preparation technical field, specifically to a kind of heparin sodium extraction equipment and extraction method, including extraction tank, stirring shaft, two stirring mechanisms and two telescopic mechanisms;Stirring shaft can rotate around the central axis of extraction tank;Two stirring mechanisms are symmetrically distributed in the two sides of stirring shaft;Two telescopic mechanisms correspond to two stirring mechanisms one by one, for driving stirring mechanism to retract along the radial direction of extraction tank, to make stirring mechanism and the inside wall of extraction tank be attached or be separated;In enzymolysis stage, stirring mechanism and the inside wall of extraction tank keep reasonable interval, avoid high viscosity small intestinal mucosa and tank wall friction to generate additional resistance;In cleaning stage, stirring mechanism is attached tank wall, and rotates along with stirring shaft circumferentially, without additional extension external scraping tool, can be continuously scraped along tank wall full circumference.

The invention provides application of eicosapentaenoic acid in preparation of drugs, health care products or feed additives for relieving oxidative stress of small intestines of dogs, and relates to the technical field of animal nutrition and health care. Eicosapentaenoic acid is used for relieving oxidative stress of small intestines of dogs, damage of oxidative stress to small intestine tissues of dogs can be relieved to a certain extent, and if the eicosapentaenoic acid is widely applied to the field of pet nursing, adverse effects caused by oxidative stress of small intestines to health of dogs can be effectively reduced. Especially when the concentration is 5 mu M, the dogs show extremely strong capability of relieving oxidative stress of small intestines of the dogs, the content of oxidation products in small intestine tissues can be remarkably reduced, the activity of antioxidant enzymes is improved, the integrity of small intestine mucosa is maintained, and the overall health level of the dogs is further improved.

A tamponade biological material for hemostasis and a preparation method therefor. The tamponade biological material is obtained by pretreating, crosslinking, and post-treating a small intestinal submucosal material. The tamponade biological material has good water resistance, a low swelling rate, and an appropriate degradation speed; it is degradable and not prone to adhesion and curling, thereby avoiding wound traction and reducing patient pain, so it is suitable for hemostasis in various situations, and has good market application prospects.