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16 results about "Ovotransferrin" patented technology
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Ovotransferrin is a glycoprotein of egg white albumen. Egg white albumen is composed of multiple proteins and ovotransferrin is the most heat reliable protein of them all. It has a molecular weight of 76,000 daltons and contains about 700 amino acids. Ovotransferrin makes up approximately 13% of egg albumen. As a member of the transferrin and metalloproteinase family, ovotransferrin has been found to produce heat shock proteins. When these heat shock proteins are induced in the skin, they provide protection against cold stress and other societal stresses. Ovotransferrin is folded in a way that forms two lobes and each lobe consists of a binding site. Each lobe is then divided into two domains of 160 amino acid residues. Its structure also consists of fifteen disulfide crosslinks and no free sulfhydryl groups. Disulfide groups stabilize the tertiary structures of proteins. Transferrins, are iron binding proteins and acute phase reactants of animal serum. It has a binding log of 15 at a pH of 7 or above, meaning that the iron binding capacity of ovotransferrin rapidly decreased at a pH that is less than 6.
Method for preparing ovotransferrin hydrolysate with high immunocompetence based on adult gastrointestinal simulated digestion system
The invention belongs to the field of food nutrition and functional factors, and particularly relates to a method for preparing ovotransferrin hydrolysate with high immunocompetence based on an adult gastrointestinal simulated digestion system. According to the method, ovotransferrin is treated through the stomach and intestine of a simulated adult, OVT hydrolysate is prepared, and the OVT hydrolysate contains HTEGSTT, TTSY and / or RTAGWVIPMG. The invention finds that the immunomodulatory activity of OVT is enhanced after gastrointestinal digestion and hydrolysis, the OVT is more easily hydrolyzed in simulated adult digestive juice than in infant digestive juice, and a contrast experiment shows that the nutrition intervention of OVT adult and infant simulated digestion products can promote the generation of short-chain fatty acids such as acetic acid, propionic acid and butyric acid; the swelling injury caused by inflammation to small intestine mucous membrane tissues is effectively relieved, so that the intestinal barrier immune function is obviously improved, and the OIA shows higher intestinal immune activity than the OIB.
Owner:WUHAN MILAI BIOTECHNOLOGY CO LTD +2
Recombinant pichia pastoris engineering bacterium, construction method thereof and application of recombinant pichia pastoris engineering bacterium in secretory expression of ovotransferrin
The invention relates to the technical field of biology, in particular to a recombinant pichia pastoris engineering bacterium, a construction method of the recombinant pichia pastoris engineering bacterium and application of the recombinant pichia pastoris engineering bacterium in secretory expression of ovotransferrin. The heterologous expression of ovotransferrin is effectively realized by optimizing ovotransferrin genes and utilizing molecular chaperone genes to strengthen the regulation and control capability of hosts, and meanwhile, by optimizing signal peptides and constructing high-copy recombinant pichia pastoris expression strains, the low-copy expression condition is verified and compared by utilizing fermentation expression, so that the heterologous expression of ovotransferrin is realized. According to the high-copy recombinant pichia pastoris engineering bacteria constructed by the invention, the ovotransferrin expression quantity is obviously improved and reaches 140 mg / L, the secretory expression efficiency of the ovotransferrin is effectively improved, and theoretical guidance is provided for heterologous expression and industrial production of the ovotransferrin.
Owner:NANJING TECH UNIV
Fermented feed for improving natural antibacterial property and antioxidant property of duck eggs
This invention discloses a fermented feed for improving the natural antibacterial and antioxidant properties of duck eggs. The fermented feed is prepared through a two-stage fermentation process using a fermentation substrate comprising the following components in parts by weight: 35-60 parts corn, 15-30 parts soybean meal, 5-15 parts wheat bran, 4-10 parts rice bran, 5-9 parts extruded flaxseed, 2-6 parts grape seed meal, 1-4 parts moringa flavonoid powder, 0.5-2.5 parts dicalcium phosphate, 0.5-3 parts limestone powder, 0.1-0.5 parts salt, and 0.1-0.8 parts compound premix. The two-stage fermentation involves first inoculating with Bacillus for aerobic fermentation, followed by inoculation with lactic acid bacteria and the addition of chitosan oligosaccharides for anaerobic fermentation. This invention significantly increases the content of active proteins such as lysozyme and ovotransferrin in duck egg white, enhancing its antibacterial properties and antioxidant capacity, and providing an efficient and economical technical path for the development of highly active duck egg white raw materials.
Owner:HUAZHONG AGRI UNIV
Method for synchronously separating ovalbumin, ovotransferrin and lysozyme from egg white
The invention discloses a method for synchronously separating ovalbumin, ovotransferrin and lysozyme from egg white, which comprises the following steps: firstly, adsorbing ovalbumin from egg white loading liquid by taking chitosan-polybutyl methacrylate nanocrystal gel with an anion exchange function as a primary separation medium, and carrying out gradient elution to obtain ovalbumin; taking a primary separation loading effluent as a secondary separation loading solution; hydroxyethyl methylacrylate-polyglycidyl methacrylate nanocrystal gel with a cation exchange function is taken as a secondary separation medium to adsorb ovotransferrin and lysozyme from a secondary separation loading solution, gradient elution is performed by gradually increasing salt concentration, and separation of ovotransferrin and lysozyme is realized. The method provided by the invention can realize synchronous separation of ovalbumin, ovotransferrin and lysozyme, has the advantages of high separation efficiency, simplicity and convenience in operation, low energy consumption and the like, and is suitable for industrial large-scale production, and the separation medium can be repeatedly used.
Owner:ZHEJIANG UNIV OF TECH
A method for preparing highly immunologically active ovotransferrin hydrolysate based on an adult gastrointestinal simulated digestive system
This invention belongs to the field of food nutrition and functional factors, specifically relating to a method for preparing highly immunomodulatory ovotransferrin (OVT) hydrolysates based on an adult gastrointestinal simulated digestive system. The method involves processing OVT using a simulated adult gastrointestinal tract to prepare OVT hydrolysates containing HTEGSTT, TTSY, and / or RTAGWVIPMG. This invention reveals that OVT exhibits enhanced immunomodulatory activity after gastrointestinal digestion and hydrolysis, and is more readily hydrolyzed in simulated adult digestive fluids than in infant digestive fluids. Comparative experiments show that nutritional intervention with OVT simulated digestive products from adults and infants can promote the production of short-chain fatty acids such as acetic acid, propionic acid, and butyric acid, effectively alleviate swelling and damage to the small intestinal mucosa caused by inflammation, significantly improve intestinal barrier immune function, and OVT IA exhibits higher intestinal immune activity than OVT IB.
Owner:WUHAN MILAI BIOTECHNOLOGY CO LTD +2
Method for improving stability of monascus pigment by preparing polysaccharide-protein-monascus pigment compound and obtained product
PendingCN120836684AFood ingredient as antioxidantFood ingredient as colourBiotechnologyRed yeast rice
The invention discloses a method for improving the stability of monascus pigment by preparing a polysaccharide-protein-monascus pigment compound and an obtained product, and relates to the technical field of food additives and natural pigment stabilization. Beta-cyclodextrin and ovotransferrin are used as stabilizers and react with the monascorubin in a certain proportion, and a ternary composite system is constructed through interaction between ovotransferrin and the monascorubin and interaction between ovotransferrin and beta-cyclodextrin so as to improve the stability of the monascorubin. And finally, the beta-cyclodextrin-ovotransferrin-monascus pigment compound is obtained. A thermal stability test and a light stability test prove that the beta-cyclodextrin and the ovotransferrin have a synergistic effect, and the thermal stability and the illumination stability of the monascus pigment can be effectively improved.
Owner:ZHONGKAI UNIV OF AGRI & ENG
Chicken serum-free culture medium for promoting proliferation of chicken primordial germ cells and use thereof
PCT designated stageWO2026097260A1Culture processCell culture active agentsPlant Germ CellsOvotransferrin
Disclosed are a chicken serum-free culture medium for promoting the proliferation of chicken primordial germ cells and use thereof. Ovotransferrin is used in the culture medium instead of chicken serum. The effect of using ovotransferrin, instead of chicken serum, in a culture system for PGCs is disclosed. Ovotransferrin can maintain the self-renewal of PGCs, thereby providing a basis for developing a stable culture system. The culture medium can avoid adverse effects caused by the undefined components of chicken serum, and the precise use of various growth factors can provide better culture conditions and reduce culture costs.
Owner:YANGZHOU UNIV
A method for extracting high solubility ovotransferrin from chicken egg white
The present application relates to the technical field of biological medicine manufacturing, and particularly relates to a method for extracting high-solubility ovotransferrin from egg white, wherein the egg white is diluted with water, FeCl3 solution is added, NaOH solution is used to adjust the pH of the solution, and high-speed shearing is performed; the pretreated egg white solution is subjected to ultrasonic and high-voltage alternating electric field treatment; anhydrous ethanol is added, the supernatant is obtained by centrifugation, and ultrafiltration is performed; water is added for concentration, and a crude ovotransferrin solution is obtained; resin is added to the crude ovotransferrin solution, citric acid solution is used to adjust the pH, and the resin is removed by filtration, so that a filtrate is obtained; PEG8000 is added, the pH is adjusted, and the precipitate is obtained by centrifugation; the precipitate is dissolved in water, and ultrafiltration is performed; water is continuously added for concentration, so that concentrated ovotransferrin is obtained. The extracted ovotransferrin has high solubility and iron binding capacity, can bind with iron elements again to prepare ovotransferrin iron preparation for improving iron deficiency anemia, the whole preparation process is green and environmentally friendly, has low cost, and can be mass-produced in an industrialized manner.
Owner:HEFEI UNIV OF TECH
Small molecule peptide for inhibiting development of ovary of blue crab and application of small molecule peptide
PendingCN121991196Aslow growth rateEffectively induces the formation ofData visualisationPeptide preparation methodsReceptorPeptide sequence
The invention belongs to the technical field of biology, and particularly relates to a small molecule peptide for inhibiting development of ovaries of blue crabs and application of the small molecule peptide. According to the method, the optimal binding conformation is screened according to the sequence prediction binding conformation of the vitellogen and the receptor of the vitellogen, the hot residue is screened according to the optimal binding conformation, and the binding energy change before and after mutation of the hot residue is calculated to determine the competitive inhibition polypeptide sequence. The competitive inhibition polypeptide can obviously slow down the development speed of the ovary of the blue crab and effectively induce the formation of the butter crab by utilizing the specificity of specific recognition of the vitellogenin of the blue crab and a receptor, so that the butter crab product can be produced in a planned manner.
Owner:SOUTHERN MARINE SCIENCE & ENGINEERING GUANGDONG LABORATORY (ZHANJIANG)
Method for improving iron binding capacity of ovotransferrin through moderate enzymolysis
The invention discloses a method for improving the iron binding capacity of ovotransferrin through moderate enzymolysis, and belongs to the technical field of food biology. The method comprises the following steps: (1) preparing raw materials: preparing natural ovotransferrin into a protein solution; (2) enzymolysis modification of ovotransferrin: setting the pH value of a protein solution system to a specific pH value, and performing stirring hydrolysis to prepare a hydrolyzed protein solution; and (3) enzyme inactivation: adjusting the pH value of the hydrolyzed protein solution to 7-9, and carrying out heating and water bath treatment to realize enzyme inactivation so as to obtain an enzymatic hydrolysate, namely the high-iron-binding active ovotransferrin enzymatic hydrolysate. According to the method disclosed by the invention, an ovotransferrin solution is subjected to proper enzymolysis reaction under the conditions of specific protease, specific pH, specific temperature, specific time and enzyme / substrate mass ratio, so that the ovotransferrin enzymolysis product with high iron binding capacity and oxidation resistance is prepared.
Owner:JIANGNAN UNIV
A synchronous production and extraction method of egg white lysozyme and ovotransferrin
This invention relates to the field of protein extraction technology, specifically disclosing a method for the simultaneous co-production and extraction of egg white lysozyme and ovotransferrin, comprising: (1) Egg white pretreatment: after dilution, adjust the pH to 4.5, filter out the ovomucin precipitate, and then adjust the supernatant to pH 8.0-9.0. (2) Cation exchange separation of lysozyme: adsorb onto a cation exchange column of the pretreatment solution, collect the permeate and eluent as the egg white flow-through solution; elute the lysozyme, and obtain the lysozyme product by concentration, desalting, sterilization, and drying. (3) Anion exchange separation of ovotransferrin: after adjusting the flow-through solution to pH 5.5-6.0, load onto an anion exchange column, elute to remove impurities, and collect the permeate containing ovotransferrin. (4) Iron removal and drying: concentrate by ultrafiltration, dialyze with citrate buffer for desalting and preliminary iron removal, then remove bound iron by chelating resin, and dry to obtain the iron-free ovotransferrin product.
Owner:INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
Recombinant bacterium for expressing ovotransferrin as well as construction method and application of recombinant bacterium
The invention provides recombinant bacteria for expressing ovotransferrin as well as a construction method and application of the recombinant bacteria, and belongs to the field of synthetic biology. The recombinant bacterium comprises a host bacterium and a recombinant expression vector, the host bacteria comprise escherichia coli, and the recombinant expression vector expresses ovotransferrin and molecular chaperonin; the molecular chaperonin comprises a trigger factor Trigger Factor and / or a GroEL-GroES compound, and the molecular chaperonin comprises a Trigger Factor and / or a GroEL-GroES compound. The GroEL-GroES compound is prepared from a GroEL protein and a GroES protein. Natural signal peptide of ovotransferrin is cut off through a gene editing technology, a recombinant expression vector capable of expressing the ovotransferrin in escherichia coli BL21 (DE3) is successfully constructed, an expression molecular chaperone is further selected for co-expression with the ovotransferrin, correct folding of the ovotransferrin is promoted, the expression efficiency is improved, and the recombinant expression vector can be used for expression of the ovotransferrin in escherichia coli BL21 (DE3). A new path is provided for biosynthesis of ovotransferrin.
Owner:SHENZHEN UNIV
Method for separating and extracting high-purity ovotransferrin
The invention relates to the technical field of protein separation and extraction, and provides a high-purity ovotransferrin separation and extraction method. According to the method, the temperature response type phosphatidylserine lipidosome compound is used for adsorbing and separating the ovotransferrin. According to the temperature response type phosphatidylserine liposome compound, the temperature response characteristic of poly (N-isopropylacrylamide) is utilized, at the temperature of 25 DEG C, a PNIPAM chain is in a stretched state, phosphatidylserine is fully exposed and can be fully combined with ovotransferrin through electrostatic and hydrophobic effects, and the adsorption effect of phosphatidylserine is greatly enhanced; when the temperature rises to 37 DEG C, the PNIPAM chain shrinks, the pore size of the liposome membrane is reduced, the adsorbed ovotransferrin can be wrapped and fixed, and the ovotransferrin is prevented from falling off in the subsequent centrifugal process. According to the adsorption and fixation mechanism under temperature regulation and control, the activity of the ovotransferrin is well protected, and an efficient and reliable method for separating and extracting the high-purity ovotransferrin is provided.
Owner:NANCHANG UNIV
Culture medium suitable for culturing ostrich cells
The disclosure provides for the culture media particularly suitable to culture primary ostrich PGCs. The culture media contain specific growth factors and compounds that provide support for the growth and proliferation of ostrich PGCs. In certain embodiments, the culture media contain a basal culture medium and β-mercaptoethanol, cholesterol, FGF1, FGF2, BMP4, and IGF1. In some embodiments, the basal culture medium contains knock-out DMEM, B27 Supplement, MEM-MEAA, L-alanyl-L-glutamine dipeptide, cytidine, guanosine, uridine, adenosine, thymidine, sodium pyruvate, ovalbumin, sodium heparin, calcium chloride, ovotransferrin, and an antibiotic. The disclosure also includes methods and kits for producing such media as well as methods of culturing cells.
Owner:COLOSSAL BIOSCIENCES INC
Bovine lactoferrin-phosvitin compound and preparation method thereof
The invention provides a bovine lactoferrin-phosvitin compound and a preparation method thereof. The phosvitin provided by the invention contains a large amount of phosphorylated serine, so that the phosvitin has relatively high negative charges, and forms a compound with a structure taking LF as a core and PSV as a shell with bovine lactoferrin through the synergistic effect of electrostatic interaction and hydrogen-bond interaction, so that the stability coefficient of the phosvitin is increased, the denaturation temperature is increased by 1-16 DEG C, and the denaturation time is shortened by 1-16 DEG C; the adverse effect of heat treatment on the lactoferrin is reduced, the heat stability of the lactoferrin is enhanced, the oxidation resistance is not affected, and the application range of the lactoferrin is expanded.
Owner:NORTHWEST A & F UNIV
CHICKEN SERUM-FREE MEDIUM FOR PROMOTING PROLIFERATION OF CHICKEN PRIMORDIAL GERM CELLS (PGCs) AND USE OF CHICKEN SERUM-FREE MEDIUM
A chicken serum-free medium for promoting proliferation of chicken primordial germ cells (PGCs) and a use of the chicken serum-free medium are provided. In the chicken serum-free medium, ovotransferrin is used instead of chicken serum. The ovotransferrin is used instead of chicken serum in a PGC culture system, and ovotransferrin can keep the self-renewing of PGCs to provide a basis for exploring a stable culture system. The chicken serum-free medium can avoid the adverse effects caused by an unclear composition of chicken serum. In addition, the accurate use of various growth factors can provide excellent culture conditions and reduce the culture cost.
Owner:YANGZHOU UNIV