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4 results about "Ovotransferrin" patented technology
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Ovotransferrin is a glycoprotein of egg white albumen. Egg white albumen is composed of multiple proteins and ovotransferrin is the most heat reliable protein of them all. It has a molecular weight of 76,000 daltons and contains about 700 amino acids. Ovotransferrin makes up approximately 13% of egg albumen. As a member of the transferrin and metalloproteinase family, ovotransferrin has been found to produce heat shock proteins. When these heat shock proteins are induced in the skin, they provide protection against cold stress and other societal stresses. Ovotransferrin is folded in a way that forms two lobes and each lobe consists of a binding site. Each lobe is then divided into two domains of 160 amino acid residues. Its structure also consists of fifteen disulfide crosslinks and no free sulfhydryl groups. Disulfide groups stabilize the tertiary structures of proteins. Transferrins, are iron binding proteins and acute phase reactants of animal serum. It has a binding log of 15 at a pH of 7 or above, meaning that the iron binding capacity of ovotransferrin rapidly decreased at a pH that is less than 6.
Fermented feed for improving natural antibacterial property and antioxidant property of duck eggs
This invention discloses a fermented feed for improving the natural antibacterial and antioxidant properties of duck eggs. The fermented feed is prepared through a two-stage fermentation process using a fermentation substrate comprising the following components in parts by weight: 35-60 parts corn, 15-30 parts soybean meal, 5-15 parts wheat bran, 4-10 parts rice bran, 5-9 parts extruded flaxseed, 2-6 parts grape seed meal, 1-4 parts moringa flavonoid powder, 0.5-2.5 parts dicalcium phosphate, 0.5-3 parts limestone powder, 0.1-0.5 parts salt, and 0.1-0.8 parts compound premix. The two-stage fermentation involves first inoculating with Bacillus for aerobic fermentation, followed by inoculation with lactic acid bacteria and the addition of chitosan oligosaccharides for anaerobic fermentation. This invention significantly increases the content of active proteins such as lysozyme and ovotransferrin in duck egg white, enhancing its antibacterial properties and antioxidant capacity, and providing an efficient and economical technical path for the development of highly active duck egg white raw materials.
Owner:HUAZHONG AGRI UNIV
A synchronous production and extraction method of egg white lysozyme and ovotransferrin
This invention relates to the field of protein extraction technology, specifically disclosing a method for the simultaneous co-production and extraction of egg white lysozyme and ovotransferrin, comprising: (1) Egg white pretreatment: after dilution, adjust the pH to 4.5, filter out the ovomucin precipitate, and then adjust the supernatant to pH 8.0-9.0. (2) Cation exchange separation of lysozyme: adsorb onto a cation exchange column of the pretreatment solution, collect the permeate and eluent as the egg white flow-through solution; elute the lysozyme, and obtain the lysozyme product by concentration, desalting, sterilization, and drying. (3) Anion exchange separation of ovotransferrin: after adjusting the flow-through solution to pH 5.5-6.0, load onto an anion exchange column, elute to remove impurities, and collect the permeate containing ovotransferrin. (4) Iron removal and drying: concentrate by ultrafiltration, dialyze with citrate buffer for desalting and preliminary iron removal, then remove bound iron by chelating resin, and dry to obtain the iron-free ovotransferrin product.
Owner:INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
Culture medium suitable for culturing ostrich cells
The disclosure provides for the culture media particularly suitable to culture primary ostrich PGCs. The culture media contain specific growth factors and compounds that provide support for the growth and proliferation of ostrich PGCs. In certain embodiments, the culture media contain a basal culture medium and β-mercaptoethanol, cholesterol, FGF1, FGF2, BMP4, and IGF1. In some embodiments, the basal culture medium contains knock-out DMEM, B27 Supplement, MEM-MEAA, L-alanyl-L-glutamine dipeptide, cytidine, guanosine, uridine, adenosine, thymidine, sodium pyruvate, ovalbumin, sodium heparin, calcium chloride, ovotransferrin, and an antibiotic. The disclosure also includes methods and kits for producing such media as well as methods of culturing cells.
Owner:COLOSSAL BIOSCIENCES INC