The invention discloses a method for extracting
ovotransferrin from pigeon eggs. The method comprises the following steps: (1) separating an
egg white solution from pigeon eggs, diluting the egg whitesolution, performing sufficient stirring, and performing
centrifugal filtration to obtain supernatant; (2) adding FeCl3.6H2O,
sodium bicarbonate and
sodium chloride into the supernatant, and continuing to perform sufficient stirring; (3) adding ethyl
alcohol into the supernatant to enable the
volume concentration of ethyl
alcohol in the solution to be 40-45 percent, and performing uniform stirring, standing and centrifuging to obtain precipitate 1 and filtrate; (4) keeping on adding ethyl
alcohol into the filtrate to enable the
volume concentration of the ethyl alcohol in the filtrate to be 55-65 percent, and performing uniform stirring, standing and centrifuging to obtain precipitate 2; and (5) mixing the precipitate 1 and the precipitate 2, washing the mixture with the ethyl alcohol, re-dissolving the mixture by adding water, mixing and stirring the mixture with resin, and performing suction
filtration,
dialysis and freeze-
drying to obtain the
ovotransferrin. The brand-new method for extracting the
ovotransferrin from pigeon eggs has relatively high extraction rate and purity.