74 results about "Anion exchange column" patented technology

The invention provides a separation and purification method of urokinase, and relates to the technical field of separation and extraction. The separation and purification method comprises the following steps: S1, regulating the pH value of male urine to 8.5, standing, and taking supernate; s2, mixing the supernate with an adsorption material, and eluting with an eluent to obtain a urokinase crude product solution; the adsorption material is a core-shell silica gel polymer, Fe3O4atSiO2-NH2 is taken as a core, and polystyrene is taken as a shell; the eluent is a sodium citrate buffer solution with the concentration of 0.5 to 0.15 M, acetonitrile with the concentration of 1 to 5 weight percent and EDTA (Ethylene Diamine Tetraacetic Acid) with the concentration of 0.5 to 1.5 mM; and S3, carrying out anion exchange column chromatography on the urokinase crude product solution, eluting with an eluent, and precipitating to obtain the urokinase. The urokinase prepared by the method has the advantages of high polymer urokinase content, high recovery rate and high activity yield.

The invention discloses an extraction method of coriolus versicolor polysaccharide. The method comprises the steps of crude extraction of the coriolus versicolor polysaccharide and purification of the coriolus versicolor polysaccharide. The crude extraction of coriolus versicolor polysaccharide comprises the following steps: water extraction and alcohol precipitation, H2O2 decoloration and freeze-drying; the purification of the coriolus versicolor polysaccharide comprises the following steps: carrying out anion exchange column chromatography on a coriolus versicolor polysaccharide crude product obtained by crude extraction of the coriolus versicolor polysaccharide, and carrying out ultrafiltration separation and purification to obtain the refined coriolus versicolor polysaccharide. The coriolus versicolor polysaccharide with the polysaccharide content of 80.18% and the protein content of 5.9% can be obtained through the preparation method. HPGPC analysis shows that the coriolus versicolor polysaccharide is uniform in molecular weight distribution, and the relative molecular weight Mw is about 17478 Da. The coriolus versicolor polysaccharide plays a role in treating the NAFLD by regulating intestinal flora and influencing bile acid metabolism, a theoretical basis is provided for treating the NAFLD by the coriolus versicolor polysaccharide, and a direction is broadened for NAFLD medicine development.

This invention belongs to the field of bile acid detection technology, and discloses a sample pretreatment method for determining the content of bile acid subtypes and its application. The pretreatment method includes the following steps: S1, acidifying the sample to be tested with an acidifying agent to a pH of 2-4 to obtain an acidified sample; S2, eluting the acidified sample onto a mixed-mode anion exchange column to obtain an eluent; S3, evaporating the eluent and reconstituted with a rehydration solution to obtain the test sample. The test sample obtained by this pretreatment method can effectively eliminate or compensate for matrix interference when used for targeted analysis of bile acid subtypes by LC-MS / MS, thus effectively improving detection sensitivity.

The application discloses a kind of acanthopanax acid polysaccharides with intestinal protection function and its preparation method and application, belong to the field of functional food and biotechnology.The acanthopanax acid polysaccharide is by water extraction alcohol precipitation, after impurity removal, through DEAE-52 anion exchange column elution and collection 0.2 mol / L NaCl elution peak, and the uniform component obtained by Sephadex G-100 gel column chromatography purification.Its uronic acid content is 30%-40%, weight average relative molecular weight is 50-100kDa, mainly by glucose, galactose, galacturonic acid and arabinose, infrared spectrum has carboxyl characteristic absorption peak at 1736 cm-1 and 1615 cm-1.Experiments prove that the acanthopanax acid polysaccharide has antioxidant activity and hypoglycemic activity.The acid polysaccharide has protective effect on LPS-induced Caco-2 cell damage, can effectively reduce the secretion of pro-inflammatory factors TNF-α, IL-1β, IL-6, and improve the level of anti-inflammatory factor IL-10.The acanthopanax acid polysaccharide can be prepared into oral liquid, tablet candy, solid beverage and other functional food forms.

This invention relates to the field of hydrometallurgical technology and discloses a method for purifying and recovering ammonium perrylate using a combination of electrodialysis and ion exchange. The method first adjusts the pH of the rhenium-containing feed solution, then selectively enriches perrylate ions using electrodialysis to obtain a high-concentration rhenium-rich solution. Subsequently, the rhenium-rich solution is subjected to deep adsorption purification through an anion exchange column. Next, rhenium is eluted from the saturated resin using an eluent to obtain a pure ammonium perrylate solution. Finally, high-purity ammonium perrylate crystals are obtained through evaporation, concentration, cooling, crystallization, and drying. Optionally, the obtained crystals can be recrystallized to further improve purity. This invention ensures the purity and process stability of the rhenium-rich solution obtained in the pre-enrichment stage, improves the overall adsorption capacity, desorption rate, and reliability of the process operation of the resin, and guarantees the stability of the final ammonium perrylate product purity and the economic benefits of the entire process recovery.

The invention discloses a method for separating fluorine-aluminum complexes in tea trees, and belongs to the technical field of separation and purification. The preparation method comprises the following steps: grinding fresh leaves, stems and roots of tea trees, and performing centrifugal separation to obtain juice; and injecting the juice into an anion exchange column for separation to obtain the form and proportion of an anionic fluorine-aluminum complex and a cationic fluorine-aluminum complex. The method is simple, high in operability and suitable for large-scale industrial use.

The application discloses radix sophorae tonkinensis polysaccharide STGP-1, a preparation method thereof and pharmaceutical use for treating lung squamous carcinoma, and relates to the technical field of medicines. The monosaccharide composition of STGP-1 includes fucose Fuc, arabinose Ara, galactose Gal, glucose Glc, xylose Xyl and mannose Man, and the molar ratio is 0.34:12.15:25.76:57.07:1.06:3.62. The STGP-1 is obtained by extraction, separation and purification from dried roots and rhizomes of radix sophorae tonkinensis, and the preparation method comprises the steps of pretreatment and crude extraction, impurity removal treatment, anion exchange column chromatography separation, gel column chromatography purification and the like. The STGP-1 can be used alone or in combination with anti-PD-1 monoclonal antibody for preparing a medicine for treating lung squamous carcinoma. The preparation process is stable, the source is natural, and the STGP-1 can provide a new high-efficiency and low-toxicity candidate medicine for immune combined treatment of lung squamous carcinoma.

The invention discloses a preparation method and application of sheep small intestine immunomodulatory protein, and belongs to the field of functional protein preparation. The preparation method comprises the following steps: carrying out phosphate buffer low-temperature low-salt extraction on freeze-dried powder of small intestines of sheep, carrying out gel column chromatography and anion exchange column chromatography separation and purification, and further carrying out high performance liquid chromatography analysis and activity evaluation to obtain the protein component with immunoregulatory activity. The sheep small intestine protein prepared by the method has no cytotoxicity, can promote the proliferation of macrophages RAW264.7 and enhance the NO release capability of cells, has an obvious promotion effect on the secretion of TNF-alpha and IL-6 by the cells, and has relatively high immunoregulation activity, so that a scientific basis is provided for the application of the sheep small intestine protein in the development of functional foods and medicines for enhancing immunoregulation; the development and utilization value of livestock by-products is improved.

The invention discloses asterias amurensis glucan as well as a preparation method and application thereof, and belongs to the technical field of biological medicine, the asterias amurensis glucan is obtained by combining an enzymolysis method with anion exchange column chromatography and gel column chromatography purification, the main chain structure of the asterias amurensis glucan is 1, 4-linked alpha-D-glucose, and the main chain structure of the asterias amurensis glucan is 1, 4-linked alpha-D-glucose. A terminal alpha-D-glucose branch chain is arranged at the O-6 position of a main chain, and meanwhile, a small amount of mannose and galactose are contained. Moreover, it is proved for the first time that the asterias amurensis glucan can serve as an intestinal barrier protective agent, and oxidative damage, caused by H2O2, of intestinal epithelial cells is improved by up-regulating an Nrf2 signal channel and tight junction protein. Therefore, the asterias amurensis glucan prepared by the invention has a huge potential of being developed into medicines and health-care products for protecting intestinal tracts.

The invention discloses a high-efficiency separation method of breast milk oligosaccharide and application of the breast milk oligosaccharide in specific recognition of influenza A virus hemagglutinin protein.The method comprises the steps that a DE-52 weak anion exchange column, a graphite carbon column and Bio-GelP10 column chromatography are combined for use, breast milk oligosaccharide components with low abundance and high molecular weight are efficiently enriched, and the specific recognition of influenza A virus hemagglutinin protein is achieved; after derivatization labeling of a bifunctional reagent 2-amino-N-(2-aminoethyl) benzamide (AEAB), 56 breast milk oligosaccharide monomers with the purity higher than 90% are separated from the isomer level through one-dimensional amide normal phase chromatography and two-dimensional C18 reverse phase circulation chromatography, and a monomer substance library is constructed. AEAB is used as a connecting arm to construct a breast milk oligosaccharide chip, an active monomer DSLNT II which is specifically combined with four subtype influenza A virus hemagglutinin proteins is screened out in a high-throughput manner, and fine structure analysis is completed in combination with electrospray tandem mass spectrometry. According to the method, the large-scale preparation of the low-abundance breast milk oligosaccharide monomer is realized, and a key material basis and theoretical guidance are provided for researching an antiviral mechanism and developing related functional foods and medicines.

The invention discloses an oligonucleotide purification method which comprises the following steps: loading an oligonucleotide crude product onto an anion exchange column, balancing by using a buffer solution, and then carrying out gradient elution on target oligonucleotide with a hydroxyl protecting group by using a mixture of the buffer solution and a buffer solution containing alkali metal halide, collecting elution fractions containing target oligonucleotides, carrying out ultrafiltration desalination, loading the elution fractions onto the anion exchange column again, balancing the elution fractions with a buffer solution, washing the anion exchange column with an acidic aqueous solution to remove hydroxyl protecting groups of the oligonucleotides, balancing the elution fractions with the buffer solution, and collecting the target oligonucleotides. And carrying out gradient elution on the target oligonucleotide with the hydroxyl protecting group removed by using a buffer solution and a buffer solution containing alkali metal halide. The oligonucleotide obtained by the purification method is high in purity, low in cost and suitable for large-scale production.

The invention discloses a preparation method of high-purity ammonium rhenate, which comprises the following steps: adding rhenium-rich slag and water into a high-pressure kettle, uniformly stirring, adding hydrogen peroxide, heating, pressurizing and leaching, precisely filtering after leaching, and standing to obtain a leaching solution; the leachate is treated through a four-stage anion exchange column, the saturated ion exchange column is resolved through high-quality ammonia water, and resolved liquid is purified and enriched ammonium rhenate liquid; and performing high-temperature thermal crystallization on the purified and enriched ammonium rhenate solution under the action of micro-pressure ultrasonic waves, performing centrifugal separation to obtain high-purity ammonium rhenate crystals, and performing high-temperature drying to obtain a high-purity ammonium rhenate product. The method is simple in process, and the prepared product is high in activity, high in crystallization rate and excellent in purity and has high application value.

The application provides a plasmid DNA extraction kit based on an anion exchange column and application thereof, and relates to the technical field of biology.The plasmid DNA extraction kit based on the anion exchange column comprises an anion exchange column, a lysis buffer group, a balance buffer, a washing buffer and an elution buffer; the anion exchange column comprises a solid-phase extraction empty column, upper and lower sieve plates arranged on the inner side of the empty column and located at the bottom of the empty column, and an anion exchange ligand filled between the upper and lower sieve plates; the anion exchange ligand comprises diethylaminoethyl dextran gel DEAE-A50 and diethylaminoethyl cellulose DE52.The application uses domestic fillers and optimizes the ratio of the fillers as the anion exchange ligand, the cost of which is only 1 / 20-1 / 40 of that of imported products, and the operation time and labor intensity are saved through the gravity flow purification process, and the obtained plasmid has high yield, and the transfection effect is equivalent to that of commercially available kits.

The invention relates to the technical field of biological medicine, and discloses a yak milk casein antioxidant peptide as well as a preparation method and application thereof, and the preparation method comprises the following steps: step 1, dissolving yak milk casein in alkali liquor to obtain a casein solution; step 2, carrying out enzymolysis on the casein solution by using protease to obtain enzymatic hydrolysate; step 3, inactivating and centrifuging the enzymatic hydrolysate, taking supernate, and freeze-drying to obtain a crude polypeptide product; and step 4, purifying the polypeptide crude product through anion exchange column chromatography and gel filtration chromatography in sequence to obtain the antioxidant peptide component. The preparation method is efficient and clear in target, yak milk casein is subjected to enzymolysis through a single enzyme / complex enzyme combined strategy, a two-step purification process of DEAE-52 cellulose column chromatography and SephadexG-50 gel chromatography is combined, the high-activity polypeptide component is efficiently and repeatedly prepared, and the defect that traditional enzymolysis product components are complex is overcome.

The invention belongs to the technical field of traditional Chinese medicine extraction, and relates to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof, in particular to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof. The purpose of the invention is to determine one component in the nine-steamed nine-processed polygonatum sibiricum product. NPCP-A1 is extracted by a water extraction and alcohol precipitation method, deproteinized by a Sevag method, and subjected to chromatographic separation and purification by a DEAE-52 anion exchange column and a Sephacryl S-300 gel column, and the NPCP-A1 is prepared from arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid. The proliferation of RAW264.7 cells and the secretion of NO, TNF-alpha and IL-6 can be promoted. The composition is used for preparing health-care products or medicines for improving immunity. According to the acidic polysaccharide prepared by the method disclosed by the invention, the polysaccharide plays an immune regulation role by promoting macrophage proliferation and increasing secretion of cell factors such as NO, TNF-alpha, IL-6 and the like.

The invention relates to the technical field of polysaccharide extraction, in particular to tremella polysaccharide for relieving blue light induced intestinal injury and a preparation method thereof, the preparation method comprises the following steps: a, extraction of tremella polysaccharide: fresh tremella is subjected to steam explosion pretreatment and then is further treated to obtain water-extracted crude tremella polysaccharide; b, separation and purification of tremella polysaccharide: carrying out graded purification on the water-extracted crude tremella polysaccharide obtained in the step a through an anion exchange column, eluting and collecting each component; and dissolving the obtained components with water, centrifuging, taking supernate, purifying by using a gel chromatographic column, eluting by using pure water, and collecting eluent, thereby obtaining the purified tremella polysaccharide. The preparation method disclosed by the invention has the beneficial effects that the cell structure of tremella fuciformis is damaged through steam explosion pretreatment, and the dissolution rate and biological activity of polysaccharide are improved; the tremella polysaccharide with high purity and good uniformity is obtained by combining anion exchange and gel chromatography purification, and has an excellent effect of relieving blue light induced intestinal injury.

The invention provides a preparation method of a high-conductivity PEDOT dispersion liquid, which comprises the following steps: (1) dispersing polyanions and a first oxidant into water, passing through an anion exchange column, removing anions, adding an EDOT monomer, fully stirring and uniformly mixing, carrying out high-pressure homogenization, and finally introducing inert gas to expel oxygen in the solution so as to obtain a reaction base solution; and (2) dissolving a second oxidant into purified water, passing through a cation exchange column, removing cations, introducing inert gas to expel oxygen in the solution so as to form a dropwise adding solution, slowly dropwise adding the dropwise adding solution into the reaction base solution, and carrying out polymerization reaction at the reaction temperature of 10-30 DEG C so as to obtain a PEDOT dispersion liquid, wherein redundant anions and cations are removed from the PEDOT dispersion liquid through anion-cation exchange resin. After the high-conductivity PEDOT dispersion liquid prepared by the method disclosed by the invention is prepared into a conductive film, the formed film is uniform, the conductivity of the film is tested by using a double-electric four-probe tester, and the conductivity is high.

The application discloses a purification method of oligonucleotide, which comprises the following steps: loading the crude oligonucleotide into an anion exchange column which is equilibrated with a phosphate buffer solution, washing the anion exchange column with the mixture of the phosphate buffer solution and the phosphate buffer solution containing alkali metal halide after equilibrating the anion exchange column with the phosphate buffer solution, equilibrating the anion exchange column with the phosphate buffer solution again, then washing the anion exchange column with an acidic aqueous solution to remove the hydroxyl protecting group of the terminal nucleoside of the oligonucleotide, and equilibrating the anion exchange column with the phosphate buffer solution, finally eluting the target oligonucleotide with the phosphate buffer solution containing alkali metal halide, and collecting the eluent containing the target oligonucleotide. The purification method of the application can efficiently and stably obtain high-purity oligonucleotide without using organic solvents, and is suitable for the purification of oligonucleotide in large-scale commercial process.

The application discloses uniform polysaccharide of radix paeoniae alba and a preparation method and application thereof, and the preparation method comprises the following steps: (1) hot extraction of radix paeoniae alba with hot water, concentration of the extraction liquid, and alcohol precipitation to obtain crude polysaccharide; (2) removal of starch and protein in the crude polysaccharide, and dialysis to obtain refined polysaccharide; (3) fractionated elution of the refined polysaccharide on a DEAE-52 anion exchange column with different concentrations of NaCl solution to obtain radix paeoniae alba polysaccharide components; and (4) elution of the polysaccharide components on a Sephadex G-75 chromatographic column with water, collection of the water eluate, and screening of the water eluate through an in-vitro anti-inflammatory activity to obtain uniform polysaccharide of radix paeoniae alba with high anti-inflammatory activity. The application is guided by the in-vitro activity screening test results to guide the separation and purification of the radix paeoniae alba polysaccharide, and the uniform polysaccharide of radix paeoniae alba with high anti-inflammatory activity is obtained through purposeful screening; and the in-vivo and in-vitro pharmacodynamic experiments prove that the prepared uniform polysaccharide has definite anti-inflammatory activity, and has application prospects in preparation of anti-inflammatory drugs and the like.

The invention discloses a preparation method of high-purity fish serum albumin, which comprises the following steps: precipitating fish serum with octanoic acid to remove impure proteins, loading the supernate to an anion exchange column after passing through a replacement buffer system, carrying out composite gradient elution, then eluting again through a dye ligand affinity chromatography column, and collecting a target protein solution, thereby obtaining the high-purity fish serum albumin. And after gel filtration chromatography, collecting a single main peak, and freeze-drying after filtration sterilization to obtain a high-purity fish serum albumin finished product. According to the method disclosed by the invention, multi-stage technologies such as octanoic acid differential precipitation, composite gradient anion exchange chromatography, dye ligand affinity chromatography column and gel filtration refined purification are organically matched, so that the problem that key impure proteins with molecular weight and isoelectric point similar to those of fish serum albumin are difficult to effectively remove in the prior art is solved; therefore, the product purity is difficult to break through 95%. According to the method, the purity of the fish serum albumin can be stably improved to 98% or above, the total recovery rate is not lower than 65%, the process route is stable, and linear amplification production is easy.

The invention relates to a total nitrosamine analyzer and a detection method thereof. The total nitrosamine analyzer comprises an anion exchange column; a liquid inlet of the atomizer is connected with a liquid outlet of the anion exchange column; a carrier gas inlet of the atomizer is connected with a carrier gas source; the ultraviolet photolysis reaction module comprises a quartz reaction tube and an ultraviolet lamp; an aerosol inlet of the quartz reaction tube is connected with an atomization outlet of the atomizer; the ultraviolet lamp is arranged corresponding to the quartz reaction tube; the chemiluminiscence detection module comprises an ozone generator and a chemiluminiscence detector, and a gas inlet of the chemiluminiscence detector is connected with a gas phase outlet of the quartz reaction tube; and the data processing module is electrically connected with the chemiluminescence detector. A to-be-detected sample solution without nitrite is obtained through the anion exchange column, the to-be-detected sample solution is converted into aerosol through the atomizer and converted into NO through the ultraviolet photolysis reaction module, the NO is detected through the chemiluminescence detection module, and the total nitrosamine concentration is calculated through the data processing module according to the detected NO.

The present invention discloses a method based on 166 Dy‑ 166 Ho generator separation 166 Ho's method, which first prepares 166 Dy / 166 Ho acid solution, then prepare the complex 166 Dy / 166 Ho‑DOTP, and finally by 166 Dy / 166 Ho-DOTP was loaded onto an anion exchange column and separated into radionuclides with a purity of more than 99% through acid activation, pure water pre-rinsing, standing, and pure water rinsing. 166 Ho. This method implements 166 The process of Ho separation is simple; pure water is used for elution. 166 Ho has no carrier, does not contain complexing molecules, and does not require further purification. 166 The radionuclide purity of Ho is greater than 99%; the DOTP used is a common chemical reagent, and its cost is significantly lower than that of DOTATOC.

The invention belongs to the technical field of biological medicine, and relates to sargassum polysaccharide as well as a preparation method and application thereof. The uniform acidic polysaccharide WZSP-2 is separated and purified from the Weizhou Sargassum through hot water extraction, ethanol precipitation, protein and color removal, anion exchange column and gel column chromatography and other methods, and the physicochemical properties, monosaccharide composition and structural morphology of the Weizhou Sargassum are systematically characterized. In-vitro experiments show that the WZSP-2 has remarkable DPPH, ABTS and hydroxyl radical scavenging capacity and total reducing power, can effectively inhibit the activity of alpha-amylase and alpha-glucosidase, can be combined with glycocholate and taurocholate, and has a bacteriostatic effect on methicillin-resistant staphylococcus aureus (MRSA) at the same time. The sargassum polysaccharide provided by the invention integrates antioxidant activity, hypoglycemic activity, hypolipidemic activity and antibacterial activity, provides a new source for developing multifunctional natural products, and has a good application prospect in the fields of functional food, health care products and medical auxiliary materials.

The present invention discloses a polysaccharide extract of Pleurotus citrinopileatus with the function of regulating microflora and promoting bowel movements, as well as a preparation method and application thereof. The present invention provides the application of the Pleurotus citrinopileatus extract in the preparation of a medicament; the uses of the medicament are as follows: (a1) treating and / or preventing constipation; (a2) promoting intestinal peristalsis; (a3) ​​promoting defecation; (a4) increasing the water content of feces; (a5) improving the structure of intestinal microflora; (a6) regulating intestinal microflora. The preparation method of the Pleurotus citrinopileatus extract is as follows: (1) using Pleurotus citrinopileatus as a raw material to extract crude polysaccharide; (2) removing protein from the crude polysaccharide; (3) sequentially performing separation and purification by anion exchange column chromatography and molecular sieve chromatography; (4) obtaining the Pleurotus citrinopileatus extract. The present invention can be used to treat and / or prevent constipation.

The invention discloses preparation and application of toad skin heparinoid sulfate capable of inhibiting activity of new spike protein. The preparation method comprises the following steps: crushing dried toad skin, heating in a water bath to swell, carrying out enzymolysis by using alkaline protease, and carrying out alcohol precipitation, anion exchange column purification, dialysis and freeze drying to obtain a toad skin glycosaminoglycan crude extract; crude toad skin glycosaminoglycan extracts are subjected to enzymolysis through chondroitin sulfate ABC enzyme, and the toad skin heparinoid sulfate is obtained through gel chromatographic column purification, desalination and freeze drying. The average molecular weight of the toad skin heparinoid sulfate is 11Kda, the toad skin heparinoid sulfate is composed of disaccharide units formed by connecting hexuronic acid and glucosamine through 1-4 glucosidic bonds, and the total sulfation degree of the C-2-oxygen site of hexuronic acid and the total sulfation degree of the C-6-oxygen site and the total sulfation degree of the N-site of glucosamine are 11.45%, 33.68% and 46.46% respectively. The toad skin heparinoid sulfate has competitive activity of inhibiting binding of new crown spike protein and heparin, and can prevent initial infection of new crown virus. The invention lays an important foundation for the development of new clinical application of the toad skin.

A radionuclide waste extraction method includes directing a waste stream from an upstream section of a main waste path to a waste stream input end of an anion exchange column, where the waste stream includes uranium and a target radionuclide, the anion exchange column containing an anion exchange resin, where the anion exchange resin is coupled to the waste stream input end of the anion exchange column. A cation exchange column containing a cation exchange resin is fluidly coupled to the anion exchange column downstream of the anion exchange column, and a rinse preparation tank is fluidly coupled to the anion exchange column. The method further includes adsorbing uranium from the waste stream onto an anion exchange resin, directing the waste stream from the anion exchange column into a cation exchange column, adsorbing radionuclides onto the cation exchange resin, removing the waste anion exchange resin from the anion exchange column, and removing uranium from the waste stream from the cation exchange column. And directing fresh anion exchange resin from the rinsed preparation tank to the anion exchange column.

The invention relates to the technical field of ion exchange, in particular to a large column ion exchange process acid-base water recovery control system and a control method thereof. By additionally arranging the acid and alkali water recovery unit, acid and alkali water meeting the concentration requirement in the regeneration process is recovered respectively, the regeneration requirements of the next group of target cation exchange columns and anion exchange columns are met, concentration grading is performed on the acid and alkali water, high-concentration waste liquid discharge is reduced, available acid and alkali resources are recovered, and the wastewater treatment cost is reduced; meanwhile, a pH monitoring module in the control unit is linked with a flow and PID module, so that the target concentration is met after the acid-base recovery water is mixed with the high-concentration acid liquor and alkali liquor in the regeneration process, and accurate control over the pH value in the regeneration process is achieved.

The invention discloses a preparation method of high-purity sugarcane leaf polysaccharide, which comprises the following steps: separating sugarcane leaf crude polysaccharide by using an alkaline H2O2 method, and then separating and purifying by using a DEAE-Sepharose Fast Flow anion exchange column to obtain the high-purity sugarcane leaf polysaccharide SLP-2. The monosaccharide composition of the high-purity sugarcane leaf polysaccharide SLP-2 is mainly Xyl, Ara and Gal, and the main chain structure of the high-purity sugarcane leaf polysaccharide SLP-2 is composed of glycosidic bonds such as Araf-(1-), Xylp-(1-),-> 3, 4)-Galp-(1-),-> 5)-Araf-(1-),-> 4)-Xylp-(1-),-> 4)-Glcp-(1-),-> 3, 4)-Xylp-(1-) and the like. The research on the efficacy of a diabetic cardiomyopathy rat model induced by feeding with streptozotocin combined with high-sugar and high-fat feed finds that the high-purity sugarcane leaf polysaccharide SLP-2 has the effects of reducing the blood sugar of the diabetic cardiomyopathy rat and improving the heart function of the diabetic cardiomyopathy rat. In conclusion, the preparation process is simple, the polysaccharide composition and structure are clear, and the SLP-2 has good effects of reducing blood sugar and improving diabetic cardiomyopathy and heart injury, has a certain application prospect in the field of medicines, and is worthy of further research.

The invention provides a plasmid DNA extraction kit based on an anion exchange column and application of the plasmid DNA extraction kit, and relates to the technical field of biology. The invention provides a plasmid DNA extraction kit based on an anion exchange column. The plasmid DNA extraction kit comprises the anion exchange column, a lysis buffer solution group, an equilibrium buffer solution, a washing buffer solution and an elution buffer solution, the anion exchange column comprises a solid-phase extraction hollow column, an upper sieve plate and a lower sieve plate which are arranged on the inner side of the hollow column and positioned at the bottom of the hollow column, and an anion exchange ligand filled between the upper sieve plate and the lower sieve plate; the anion exchange ligand is prepared from diethylaminoethyl sephadex (DEAE)-A50 and diethylaminoethyl cellulose (DE52. According to the present invention, the domestic filler and the optimized filler ratio are adopted as the anion exchange ligand, the cost is only 1 / 20-1 / 40 of the cost of the imported product, the operation time and the labor intensity are saved through the gravity flow purification process, the yield of the obtained plasmid is high, and the transfection effect is equivalent to the transfection effect of the commercially available kit.