96 results about "Anion exchange column" patented technology

The invention provides a method for detecting the concentration of phosphite ions in a natural water medium, which comprises the following steps: detecting a to-be-detected sample solution containing phosphite ions and impurity ions by using an ion chromatograph to obtain a chromatogram of the to-be-detected sample; determining a peak area value of phosphite ions from the chromatogram of the sample to be detected; obtaining the concentration of the phosphite ions in the sample to be detected according to a linear regression equation of the peak area value of the phosphite ions and the concentration of the phosphite ions; wherein the test conditions of the ion chromatograph are as follows: an anion exchange column is selected as a chromatographic column, and the column temperature is 43-56 DEG C; the leacheate is a potassium hydroxide solution, the flow rate is 1.0-2.0 mL / min, gradient leaching is adopted, and the concentration of the leacheate is 15-18 mM within the time period of 0-14 min; the concentration of the leacheate is 33-36 mM within the time period of 16-30 min; the concentration of the leacheate is 17 to 20 mM within the time period of 32 to 36 minutes.

The invention discloses a preparation method of sargassum fusiforme low-molecular-weight fucoidin with an auxiliary protection effect on chemical liver injury. The preparation method comprises the following steps: S1, degreasing treatment; s2, preparing crude polysaccharide; s3, degradation of crude polysaccharide: dissolving the sargassum fucoidin crude product with water to obtain a polysaccharide solution, and performing ultrasonic treatment; then, H2O2 is added for degradation, and the sargassum fusiforme low-molecular-weight fucoidin DSFF is obtained; and S4, purification: dissolving the sargassum fusiforme low-molecular-weight fucoidin DSFF in water to obtain a polysaccharide solution, loading the polysaccharide solution on a DEAE-52 cellulose anion exchange column, eluting with a NaCl-phosphate buffer solution, dialyzing and desalting the eluent, and freeze-drying to obtain the sargassum fusiforme low-molecular-weight fucoidin DSFF2. The sargassum fusiforme low-molecular-weight fucoidin prepared by the method can be used for preparing medicines with an auxiliary protection effect on chemical liver injury. The research of the invention lays a foundation for the development of liver protection products related to sargassum fucoidin.

The invention provides a separation and purification method of urokinase, and relates to the technical field of separation and extraction. The separation and purification method comprises the following steps: S1, regulating the pH value of male urine to 8.5, standing, and taking supernate; s2, mixing the supernate with an adsorption material, and eluting with an eluent to obtain a urokinase crude product solution; the adsorption material is a core-shell silica gel polymer, Fe3O4atSiO2-NH2 is taken as a core, and polystyrene is taken as a shell; the eluent is a sodium citrate buffer solution with the concentration of 0.5 to 0.15 M, acetonitrile with the concentration of 1 to 5 weight percent and EDTA (Ethylene Diamine Tetraacetic Acid) with the concentration of 0.5 to 1.5 mM; and S3, carrying out anion exchange column chromatography on the urokinase crude product solution, eluting with an eluent, and precipitating to obtain the urokinase. The urokinase prepared by the method has the advantages of high polymer urokinase content, high recovery rate and high activity yield.

The invention discloses an extraction method of coriolus versicolor polysaccharide. The method comprises the steps of crude extraction of the coriolus versicolor polysaccharide and purification of the coriolus versicolor polysaccharide. The crude extraction of coriolus versicolor polysaccharide comprises the following steps: water extraction and alcohol precipitation, H2O2 decoloration and freeze-drying; the purification of the coriolus versicolor polysaccharide comprises the following steps: carrying out anion exchange column chromatography on a coriolus versicolor polysaccharide crude product obtained by crude extraction of the coriolus versicolor polysaccharide, and carrying out ultrafiltration separation and purification to obtain the refined coriolus versicolor polysaccharide. The coriolus versicolor polysaccharide with the polysaccharide content of 80.18% and the protein content of 5.9% can be obtained through the preparation method. HPGPC analysis shows that the coriolus versicolor polysaccharide is uniform in molecular weight distribution, and the relative molecular weight Mw is about 17478 Da. The coriolus versicolor polysaccharide plays a role in treating the NAFLD by regulating intestinal flora and influencing bile acid metabolism, a theoretical basis is provided for treating the NAFLD by the coriolus versicolor polysaccharide, and a direction is broadened for NAFLD medicine development.

The invention relates to a cyclic utilization method of a maleic anhydride epoxidation catalyst. The method comprises the following steps: completely introducing a synthesized epoxy succinic acid reaction solution into an anion exchange column, adsorbing anions of a catalyst by using anion exchange resin, leaching the anion exchange column by using pure water, applying leacheate and the adsorbed reaction solution to synthesis of polyepoxysuccinic acid, adding quantitative maleic anhydride and water into a reaction kettle, and reacting for 2-4 hours at the temperature of 60-80 DEG C; and dropwise adding liquid alkali for hydrolysis, adding anion resin adsorbing the catalyst, heating, dropwise adding an aqueous hydrogen peroxide solution, then carrying out suction filtration, separating the anion resin from the epoxy succinic acid reaction solution, and continuously using the obtained anion resin and epoxy succinic acid reaction solution according to the steps to complete the recycling of the catalyst. According to the method, the specific anion resin is used for adsorbing the catalyst in the reaction system, and the catalyst and the adsorption resin can be recycled for multiple times only by adding the resin adsorbing the tungsten and molybdenum catalysts into the reaction system.

The invention belongs to the technical field of biology, and particularly relates to a preparation method of peony exosomes. The preparation method of the peony exosome comprises the following steps: crushing peony plants, filtering, and collecting supernatant to obtain first supernatant; carrying out anion exchange column chromatography on the first supernatant, taking a PBS (Phosphate Buffer Solution) as an elution buffer solution, and collecting a fourth cut-off branch effluent; performing freeze drying on the fourth cut-off branch effluent to obtain freeze-dried powder; dissolving the freeze-dried powder with a PBS buffer solution; obtaining a freeze-dried solution; purifying the freeze-dried solution through sephadex column chromatography, eluting and collecting to obtain a second supernatant; and filtering the second supernate through a microporous filter membrane to obtain the peony exosome. The preparation method of the peony exosome provided by the invention not only obviously improves the extraction efficiency of the peony exosome, but also has the advantages of simplicity and convenience in operation and cost saving, and lays a solid foundation for wide application of the peony exosome in industrial production.

This invention belongs to the field of bile acid detection technology, and discloses a sample pretreatment method for determining the content of bile acid subtypes and its application. The pretreatment method includes the following steps: S1, acidifying the sample to be tested with an acidifying agent to a pH of 2-4 to obtain an acidified sample; S2, eluting the acidified sample onto a mixed-mode anion exchange column to obtain an eluent; S3, evaporating the eluent and reconstituted with a rehydration solution to obtain the test sample. The test sample obtained by this pretreatment method can effectively eliminate or compensate for matrix interference when used for targeted analysis of bile acid subtypes by LC-MS / MS, thus effectively improving detection sensitivity.

The application discloses a kind of acanthopanax acid polysaccharides with intestinal protection function and its preparation method and application, belong to the field of functional food and biotechnology.The acanthopanax acid polysaccharide is by water extraction alcohol precipitation, after impurity removal, through DEAE-52 anion exchange column elution and collection 0.2 mol / L NaCl elution peak, and the uniform component obtained by Sephadex G-100 gel column chromatography purification.Its uronic acid content is 30%-40%, weight average relative molecular weight is 50-100kDa, mainly by glucose, galactose, galacturonic acid and arabinose, infrared spectrum has carboxyl characteristic absorption peak at 1736 cm-1 and 1615 cm-1.Experiments prove that the acanthopanax acid polysaccharide has antioxidant activity and hypoglycemic activity.The acid polysaccharide has protective effect on LPS-induced Caco-2 cell damage, can effectively reduce the secretion of pro-inflammatory factors TNF-α, IL-1β, IL-6, and improve the level of anti-inflammatory factor IL-10.The acanthopanax acid polysaccharide can be prepared into oral liquid, tablet candy, solid beverage and other functional food forms.

This invention relates to the field of hydrometallurgical technology and discloses a method for purifying and recovering ammonium perrylate using a combination of electrodialysis and ion exchange. The method first adjusts the pH of the rhenium-containing feed solution, then selectively enriches perrylate ions using electrodialysis to obtain a high-concentration rhenium-rich solution. Subsequently, the rhenium-rich solution is subjected to deep adsorption purification through an anion exchange column. Next, rhenium is eluted from the saturated resin using an eluent to obtain a pure ammonium perrylate solution. Finally, high-purity ammonium perrylate crystals are obtained through evaporation, concentration, cooling, crystallization, and drying. Optionally, the obtained crystals can be recrystallized to further improve purity. This invention ensures the purity and process stability of the rhenium-rich solution obtained in the pre-enrichment stage, improves the overall adsorption capacity, desorption rate, and reliability of the process operation of the resin, and guarantees the stability of the final ammonium perrylate product purity and the economic benefits of the entire process recovery.

The invention discloses a method for separating fluorine-aluminum complexes in tea trees, and belongs to the technical field of separation and purification. The preparation method comprises the following steps: grinding fresh leaves, stems and roots of tea trees, and performing centrifugal separation to obtain juice; and injecting the juice into an anion exchange column for separation to obtain the form and proportion of an anionic fluorine-aluminum complex and a cationic fluorine-aluminum complex. The method is simple, high in operability and suitable for large-scale industrial use.

The invention relates to the technical field of circulating water treatment, in particular to a method for jointly reducing COD (Chemical Oxygen Demand) and hardness of chloride ions and calcium and magnesium in circulating water. Circulating water firstly passes through a multi-medium filter to reduce the turbidity in the circulating water, then the circulating water enters an anion exchange column, chloride ions, sulfate radicals and other inorganic anions and oxalate, formate, benzoate radicals, acetate and other organic anions contributing COD in the circulating water are replaced with hydroxyl radicals, and at the moment, the pH of outlet water of the circulating water is 13-14; effluent of the exchange column enters a sloping plate sedimentation tank, 4% sulfuric acid is added into an inlet of the sedimentation tank to adjust the pH to be 7-8.5, and effluent of the sloping plate sedimentation tank is purified circulating water. According to the method, the scaling possibility of the circulating water is effectively reduced, the water quality of the circulating water can be remarkably improved, for example, after the circulating water is adsorbed by the ion exchange column, the COD removal rate is greater than 85%, the chloride ion removal rate is higher than 80%, and after the circulating water is settled by adding sulfuric acid into the inclined plate settling tank, the calcium and magnesium hardness removal rate is not lower than 50%.

The invention provides a preparation method of nonapeptide-1, which comprises the following steps: firstly synthesizing a nonapeptide-1 crude product by a solid-phase synthesis method, and then purifying by adopting a mode of combining a modified polysulfone filter membrane, a reversed-phase polymer column and a weak anion exchange column, so that the purification efficiency is greatly improved. By controlling the dosage of an acylation reagent, the modified polysulfone filter membrane achieves the effect of partial hydrophilic modification, amide groups are grafted on the surface and micropores of the filter membrane, and meanwhile, a large number of amino groups are reserved, so that the compatibility of the modified polysulfone filter membrane and polypeptide is greatly enhanced, the permeability of polypeptide molecules is enhanced, and the service life of the modified polysulfone filter membrane is prolonged. Polypeptide molecules can be quickly and efficiently separated from impurities, so that the recovery rate of polypeptide is increased; meanwhile, quaternary ammonium graphene oxide is added into modified polysulfone through a blending and phase inversion method, and then the membrane is formed through coating and casting, so that the hydrophilicity of the modified polysulfone filter membrane is effectively improved, the modified polysulfone filter membrane has a synergistic effect with amide groups, the permeability of polypeptide molecules is further improved, and the recovery rate of polypeptide is increased.

The application discloses radix sophorae tonkinensis polysaccharide STGP-1, a preparation method thereof and pharmaceutical use for treating lung squamous carcinoma, and relates to the technical field of medicines. The monosaccharide composition of STGP-1 includes fucose Fuc, arabinose Ara, galactose Gal, glucose Glc, xylose Xyl and mannose Man, and the molar ratio is 0.34:12.15:25.76:57.07:1.06:3.62. The STGP-1 is obtained by extraction, separation and purification from dried roots and rhizomes of radix sophorae tonkinensis, and the preparation method comprises the steps of pretreatment and crude extraction, impurity removal treatment, anion exchange column chromatography separation, gel column chromatography purification and the like. The STGP-1 can be used alone or in combination with anti-PD-1 monoclonal antibody for preparing a medicine for treating lung squamous carcinoma. The preparation process is stable, the source is natural, and the STGP-1 can provide a new high-efficiency and low-toxicity candidate medicine for immune combined treatment of lung squamous carcinoma.

The invention discloses a preparation method and application of sheep small intestine immunomodulatory protein, and belongs to the field of functional protein preparation. The preparation method comprises the following steps: carrying out phosphate buffer low-temperature low-salt extraction on freeze-dried powder of small intestines of sheep, carrying out gel column chromatography and anion exchange column chromatography separation and purification, and further carrying out high performance liquid chromatography analysis and activity evaluation to obtain the protein component with immunoregulatory activity. The sheep small intestine protein prepared by the method has no cytotoxicity, can promote the proliferation of macrophages RAW264.7 and enhance the NO release capability of cells, has an obvious promotion effect on the secretion of TNF-alpha and IL-6 by the cells, and has relatively high immunoregulation activity, so that a scientific basis is provided for the application of the sheep small intestine protein in the development of functional foods and medicines for enhancing immunoregulation; the development and utilization value of livestock by-products is improved.

The invention discloses asterias amurensis glucan as well as a preparation method and application thereof, and belongs to the technical field of biological medicine, the asterias amurensis glucan is obtained by combining an enzymolysis method with anion exchange column chromatography and gel column chromatography purification, the main chain structure of the asterias amurensis glucan is 1, 4-linked alpha-D-glucose, and the main chain structure of the asterias amurensis glucan is 1, 4-linked alpha-D-glucose. A terminal alpha-D-glucose branch chain is arranged at the O-6 position of a main chain, and meanwhile, a small amount of mannose and galactose are contained. Moreover, it is proved for the first time that the asterias amurensis glucan can serve as an intestinal barrier protective agent, and oxidative damage, caused by H2O2, of intestinal epithelial cells is improved by up-regulating an Nrf2 signal channel and tight junction protein. Therefore, the asterias amurensis glucan prepared by the invention has a huge potential of being developed into medicines and health-care products for protecting intestinal tracts.

The invention discloses a high-efficiency separation method of breast milk oligosaccharide and application of the breast milk oligosaccharide in specific recognition of influenza A virus hemagglutinin protein.The method comprises the steps that a DE-52 weak anion exchange column, a graphite carbon column and Bio-GelP10 column chromatography are combined for use, breast milk oligosaccharide components with low abundance and high molecular weight are efficiently enriched, and the specific recognition of influenza A virus hemagglutinin protein is achieved; after derivatization labeling of a bifunctional reagent 2-amino-N-(2-aminoethyl) benzamide (AEAB), 56 breast milk oligosaccharide monomers with the purity higher than 90% are separated from the isomer level through one-dimensional amide normal phase chromatography and two-dimensional C18 reverse phase circulation chromatography, and a monomer substance library is constructed. AEAB is used as a connecting arm to construct a breast milk oligosaccharide chip, an active monomer DSLNT II which is specifically combined with four subtype influenza A virus hemagglutinin proteins is screened out in a high-throughput manner, and fine structure analysis is completed in combination with electrospray tandem mass spectrometry. According to the method, the large-scale preparation of the low-abundance breast milk oligosaccharide monomer is realized, and a key material basis and theoretical guidance are provided for researching an antiviral mechanism and developing related functional foods and medicines.

The invention discloses an oligonucleotide purification method which comprises the following steps: loading an oligonucleotide crude product onto an anion exchange column, balancing by using a buffer solution, and then carrying out gradient elution on target oligonucleotide with a hydroxyl protecting group by using a mixture of the buffer solution and a buffer solution containing alkali metal halide, collecting elution fractions containing target oligonucleotides, carrying out ultrafiltration desalination, loading the elution fractions onto the anion exchange column again, balancing the elution fractions with a buffer solution, washing the anion exchange column with an acidic aqueous solution to remove hydroxyl protecting groups of the oligonucleotides, balancing the elution fractions with the buffer solution, and collecting the target oligonucleotides. And carrying out gradient elution on the target oligonucleotide with the hydroxyl protecting group removed by using a buffer solution and a buffer solution containing alkali metal halide. The oligonucleotide obtained by the purification method is high in purity, low in cost and suitable for large-scale production.

The invention discloses a preparation method of high-purity ammonium rhenate, which comprises the following steps: adding rhenium-rich slag and water into a high-pressure kettle, uniformly stirring, adding hydrogen peroxide, heating, pressurizing and leaching, precisely filtering after leaching, and standing to obtain a leaching solution; the leachate is treated through a four-stage anion exchange column, the saturated ion exchange column is resolved through high-quality ammonia water, and resolved liquid is purified and enriched ammonium rhenate liquid; and performing high-temperature thermal crystallization on the purified and enriched ammonium rhenate solution under the action of micro-pressure ultrasonic waves, performing centrifugal separation to obtain high-purity ammonium rhenate crystals, and performing high-temperature drying to obtain a high-purity ammonium rhenate product. The method is simple in process, and the prepared product is high in activity, high in crystallization rate and excellent in purity and has high application value.

The application provides a plasmid DNA extraction kit based on an anion exchange column and application thereof, and relates to the technical field of biology.The plasmid DNA extraction kit based on the anion exchange column comprises an anion exchange column, a lysis buffer group, a balance buffer, a washing buffer and an elution buffer; the anion exchange column comprises a solid-phase extraction empty column, upper and lower sieve plates arranged on the inner side of the empty column and located at the bottom of the empty column, and an anion exchange ligand filled between the upper and lower sieve plates; the anion exchange ligand comprises diethylaminoethyl dextran gel DEAE-A50 and diethylaminoethyl cellulose DE52.The application uses domestic fillers and optimizes the ratio of the fillers as the anion exchange ligand, the cost of which is only 1 / 20-1 / 40 of that of imported products, and the operation time and labor intensity are saved through the gravity flow purification process, and the obtained plasmid has high yield, and the transfection effect is equivalent to that of commercially available kits.

The invention relates to the technical field of biological medicine, and discloses a yak milk casein antioxidant peptide as well as a preparation method and application thereof, and the preparation method comprises the following steps: step 1, dissolving yak milk casein in alkali liquor to obtain a casein solution; step 2, carrying out enzymolysis on the casein solution by using protease to obtain enzymatic hydrolysate; step 3, inactivating and centrifuging the enzymatic hydrolysate, taking supernate, and freeze-drying to obtain a crude polypeptide product; and step 4, purifying the polypeptide crude product through anion exchange column chromatography and gel filtration chromatography in sequence to obtain the antioxidant peptide component. The preparation method is efficient and clear in target, yak milk casein is subjected to enzymolysis through a single enzyme / complex enzyme combined strategy, a two-step purification process of DEAE-52 cellulose column chromatography and SephadexG-50 gel chromatography is combined, the high-activity polypeptide component is efficiently and repeatedly prepared, and the defect that traditional enzymolysis product components are complex is overcome.

The invention belongs to the technical field of traditional Chinese medicine extraction, and relates to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof, in particular to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof. The purpose of the invention is to determine one component in the nine-steamed nine-processed polygonatum sibiricum product. NPCP-A1 is extracted by a water extraction and alcohol precipitation method, deproteinized by a Sevag method, and subjected to chromatographic separation and purification by a DEAE-52 anion exchange column and a Sephacryl S-300 gel column, and the NPCP-A1 is prepared from arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid. The proliferation of RAW264.7 cells and the secretion of NO, TNF-alpha and IL-6 can be promoted. The composition is used for preparing health-care products or medicines for improving immunity. According to the acidic polysaccharide prepared by the method disclosed by the invention, the polysaccharide plays an immune regulation role by promoting macrophage proliferation and increasing secretion of cell factors such as NO, TNF-alpha, IL-6 and the like.

The invention discloses a lotus acidic polysaccharide in the technical field of extraction of plant polysaccharides, and the lotus acidic polysaccharide is mainly composed of rhamnose, glucuronic acid, galacturonic acid, galactose and arabinose with the molar percentage of 4-10%: 1-3%: 60-85%: 5-10%: 3-8%. The polysaccharide is obtained by adopting a water extraction and alcohol precipitation method and anion exchange column separation and purification. Researches show that the lotus acidic polysaccharide can be used for preventing and / or treating hepatic fibrosis.

The invention relates to a method for separating mannitol and sodium gluconate in mannitol fermentation liquor, which comprises the following steps: the fermentation liquor firstly passes through a strongly acidic cation exchange column, sodium ions are adsorbed on resin of the cation exchange column when the fermentation liquor passes through the cation exchange column, and then the fermentation liquor passes through a carbon column for decoloration; the mannitol is separated from an anion exchange column by controlling the discharge conductivity and the pH value through the alkalescent anion exchange column; and analyzing the alkalescent anion exchange column by using a NaOH solution to obtain sodium gluconate, and finally separating the mannitol from the sodium gluconate in the mannitol fermentation liquor. Wherein the anion resin column and the anion resin column can be regenerated and recycled after being used, so that the production cost is reduced. The yield of the separated mannitol is 93% or above, and a good separation effect is achieved.

The invention relates to the technical field of polysaccharide extraction, in particular to tremella polysaccharide for relieving blue light induced intestinal injury and a preparation method thereof, the preparation method comprises the following steps: a, extraction of tremella polysaccharide: fresh tremella is subjected to steam explosion pretreatment and then is further treated to obtain water-extracted crude tremella polysaccharide; b, separation and purification of tremella polysaccharide: carrying out graded purification on the water-extracted crude tremella polysaccharide obtained in the step a through an anion exchange column, eluting and collecting each component; and dissolving the obtained components with water, centrifuging, taking supernate, purifying by using a gel chromatographic column, eluting by using pure water, and collecting eluent, thereby obtaining the purified tremella polysaccharide. The preparation method disclosed by the invention has the beneficial effects that the cell structure of tremella fuciformis is damaged through steam explosion pretreatment, and the dissolution rate and biological activity of polysaccharide are improved; the tremella polysaccharide with high purity and good uniformity is obtained by combining anion exchange and gel chromatography purification, and has an excellent effect of relieving blue light induced intestinal injury.

The invention provides a preparation method of a high-conductivity PEDOT dispersion liquid, which comprises the following steps: (1) dispersing polyanions and a first oxidant into water, passing through an anion exchange column, removing anions, adding an EDOT monomer, fully stirring and uniformly mixing, carrying out high-pressure homogenization, and finally introducing inert gas to expel oxygen in the solution so as to obtain a reaction base solution; and (2) dissolving a second oxidant into purified water, passing through a cation exchange column, removing cations, introducing inert gas to expel oxygen in the solution so as to form a dropwise adding solution, slowly dropwise adding the dropwise adding solution into the reaction base solution, and carrying out polymerization reaction at the reaction temperature of 10-30 DEG C so as to obtain a PEDOT dispersion liquid, wherein redundant anions and cations are removed from the PEDOT dispersion liquid through anion-cation exchange resin. After the high-conductivity PEDOT dispersion liquid prepared by the method disclosed by the invention is prepared into a conductive film, the formed film is uniform, the conductivity of the film is tested by using a double-electric four-probe tester, and the conductivity is high.

The application discloses a purification method of oligonucleotide, which comprises the following steps: loading the crude oligonucleotide into an anion exchange column which is equilibrated with a phosphate buffer solution, washing the anion exchange column with the mixture of the phosphate buffer solution and the phosphate buffer solution containing alkali metal halide after equilibrating the anion exchange column with the phosphate buffer solution, equilibrating the anion exchange column with the phosphate buffer solution again, then washing the anion exchange column with an acidic aqueous solution to remove the hydroxyl protecting group of the terminal nucleoside of the oligonucleotide, and equilibrating the anion exchange column with the phosphate buffer solution, finally eluting the target oligonucleotide with the phosphate buffer solution containing alkali metal halide, and collecting the eluent containing the target oligonucleotide. The purification method of the application can efficiently and stably obtain high-purity oligonucleotide without using organic solvents, and is suitable for the purification of oligonucleotide in large-scale commercial process.

The present invention discloses a method based on 140 Nd‑ 140 Pr generator separation 140 Pr method, which first prepares the complex 140 Nd / 140 Pr‑DOTP, then the complex 140 Nd / 140 Pr-DOTP was loaded onto an anion exchange column and separated through acid activation, pure water pre-rinsing, standing, and pure water rinsing. 140 Pr. This method implements 140 The process of Pr separation is simple, using pure water for elution. 140 Pr has no carrier, does not contain complexing molecules, and does not require further purification. The DOTP used is a common chemical reagent, and its cost is significantly lower than that of DOTATOC.

The invention discloses a method for removing endotoxin in biosynthetic recombinant small RNA (Ribonucleic Acid), which comprises the following steps of: separating and purifying total RNA expressed by escherichia coli in FPLC (Fluorescence Programmable Logic Controller) by using an anion exchange column, desalting and concentrating by using an ultrafiltration membrane, and removing the endotoxin in a recombinant small RNA solution by using an ultrafiltration method. Meanwhile, the effects of different endotoxin removal methods are also compared, and finally, the effects of different methods are comprehensively evaluated from the three aspects of sample flux, the recovery rate of the recombinant small RNA and the endotoxin removal rate. Other impurities are not introduced in the process of removing the endotoxin by the ultrafiltration method, the flux is high, the durability is good, the stability is excellent, and the content of the endotoxin is 1.2-5.8 EU / ml, which is lower than the endotoxin limit requirement of biosynthetic protein preparations similar to the production process in Pharmacopoeia. The invention provides an economical, efficient and rapid method for removing the endotoxin of the recombinant small RNA, and powerful support is provided for wide application of the recombinant small RNA.

The application discloses uniform polysaccharide of radix paeoniae alba and a preparation method and application thereof, and the preparation method comprises the following steps: (1) hot extraction of radix paeoniae alba with hot water, concentration of the extraction liquid, and alcohol precipitation to obtain crude polysaccharide; (2) removal of starch and protein in the crude polysaccharide, and dialysis to obtain refined polysaccharide; (3) fractionated elution of the refined polysaccharide on a DEAE-52 anion exchange column with different concentrations of NaCl solution to obtain radix paeoniae alba polysaccharide components; and (4) elution of the polysaccharide components on a Sephadex G-75 chromatographic column with water, collection of the water eluate, and screening of the water eluate through an in-vitro anti-inflammatory activity to obtain uniform polysaccharide of radix paeoniae alba with high anti-inflammatory activity. The application is guided by the in-vitro activity screening test results to guide the separation and purification of the radix paeoniae alba polysaccharide, and the uniform polysaccharide of radix paeoniae alba with high anti-inflammatory activity is obtained through purposeful screening; and the in-vivo and in-vitro pharmacodynamic experiments prove that the prepared uniform polysaccharide has definite anti-inflammatory activity, and has application prospects in preparation of anti-inflammatory drugs and the like.