438 results about "Anion exchange column" patented technology

A method of chemically extracting radium-228, rare earth metals, thorium, the decay products of thorium, and phosphates from thorium-containing ores. The method involves breaking thorium-containing ore into fragments, wetting the fragments with a concentrated strong acid to make a slurry, heating the slurry, passing the heated solution through a first anion exchange column, retaining metals and radium-228 captured on the resin, allowing the radium-228 ions to decay to actinium-228, purifying the actinium-228 fraction, sending the actinium-228 fraction through a capture column, eluting the captured thorium-228 with acid, removing radium from the solution, retaining the radium-228 fraction for isomer in-growth, retaining decay products from the radium-228, separating the REEs from the process stream; and eluting and retaining the REEs.

Aiming at the conditions that heparin has severe bleeding side effects in clinical practice and clinical application thereof is restricted, the invention discloses a technique for producing ultra-low molecular heparin sodium (calcium). The technique comprises the following steps of: taking heparin sodium solution, adding sodium nitrite solution for cracking; adjusting the lysis buffer by using alkaline; absorbing impurities by using an anion-exchange column; washing for obtaining ultra-low molecular heparin calcium; carrying out filtration by using an ultrafiltration membrane and obtaining a precipitate by using alcohol; and after desalting, dehydration, re-precipitation, cooling and drying, obtaining a finished product of ultra-low molecular heparin calcium. The product has better and safer antithrombotic effect under low level of anticoagulation, and can be widely used for preventing and treating diseases such as deep vein thrombosis, pulmonary embolism, disseminated intravascular coagulation, and the like.

The invention relates to a method for separating and purifying human immunoglobulin from a component I+III of blood plasma, and aims to provide a high-efficiency method for recovering high-purity human immunoglobulin. According to the technical scheme provided by the invention, the method comprises the following steps of: a, fully dissolving component I+III precipitate; b, precipitating with octylic acid and removing lipid and a part of impurity protein to prepare IgG (Immunoglobulin G); c, purifying through anion exchange column chromatography; and d, collecting flow-through liquid, performing membrane nanofiltration, ultrafiltration and concentration, preparing the human immunoglobulin, sterilizing and packaging. The method has the beneficial effects of capability of being operated at the room temperature, simple and short steps, high yield, low energy consumption and high output and is suitable for mass production; comprehensive utilization of the blood plasma is fully realized; the time of the entire production process is shortened; the cost is reduced; extremely considerable economic benefit can be produced; the safety of a product is guaranteed by using two virus inactivation/elimination methods of different mechanisms; the environmental pollution is avoided; and the method has high economic and social values.

The invention relates to a determination method for inorganic arsenic in aquatic products. After being extracted by hydrochloric acid solution, the inorganic arsenic (1plus1) in the aquatic products passes through an anion-exchange column, and arsenite As (III), dimethyl arsenic compound DMA, methyl arsenic compound MMA and arsenate As (V) are eluted sequentially by mobile phase; owing to different adsorption capacities of the anion-exchange column on the arsenate As (V), the arsenite As (III), the methyl arsenic compound MMA, the dimethyl arsenic compound DMA and arsenic sugar AsS, the eluted solution is hydrogenated by borohydride potassium reducing agent and hydrochloride, and hydride is generated, enters an atomizer, and is subject to analytic determination by being combined with atomic fluorescence. The experimental condition is reasonably selected, and the detecting data is accurate and reliable and can not be influenced by extraction time and temperature. The determination method of inorganic arsenic in aquatic products of the invention not only can detect the inorganic arsenic iAs accurately, but also can determine the methyl arsenic compound MMA and the dimethyl arsenic compound DMA of the organic arsenic, and can be used for the morphometry of the arsenic in the aquatic products.

The invention relates to a method for extracting sea cucumber polysaccharide by utilizing sea cucumber processed waste liquor. The method comprises the steps that firstly, the alcohol precipitation is performed on sea cucumber processed waste liquor after being decompressed and condensed at first time, impurities are removed through centrifugation, and the first supernatant liquid is collected, decompressed and condensed at second time to obtain concentrated solution; secondly, the obtained concentrated solution is adsorbed through a macroporous resin column to remove sea cucumber saponin, and to obtain the first filtered liquid; thirdly, the alcohol precipitation is performed on the first filtered liquid, precipitation is collected through centrifugation and is added with pure water to be redissolved, the insoluble part is removed through centrifugation, to obtain the second supernatant liquid; saline matter is removed from the second supernatant liquid through a cation exchange column and an anion exchange column, to obtain the second filtered liquid; and fourthly, the second filtered liquid is dried to obtain the sea cucumber polysaccharide dry product.

The invention discloses a method for recycling heavy metal resources of stainless steel pickling waste water neutralization sludge and belongs to the technical field of recycling of sludge after sewage treatment. The method comprises the following steps of: 1) performing acid pickling, namely extracting the stainless steel pickling waste water neutralization sludge by using sulfuric acid as extractant, and adding an additive to inhibit the leaching of ferrum; 2) oxidizing, namely adding oxidant into a leaching solution to oxidize Mn(II) into manganese dioxide; 3) performing ion exchange, allowing a solution obtained after oxidation to pass through an anion exchange column, enriching and recycling hexavalent chromium, adsorbing and saturating, regenerating by using regenerant, and recycling chromate from a regeneration solution; and 4) performing neutralization precipitation, namely recycling nickelous hydroxide from ion exchange effluent by a neutralization precipitation method. By the method, ferrum is inhibited at an acid pickling section, the acid pickling is performed while ferrum is removed, the multi-stage multi-step recycling of manganese, chromium, and nickel metal resources can be realized, the innocent treatment of sludge and recycling of heavy metal are realized, and the method has comprehensive benefit of economic benefit, environmental benefit and social benefit.

The present invention belongs to the field of medicine and health care article amino acid, and includes pre-treatment of the fermented liquid to eliminate thallus, partial protein, pigment and polysaccharide, controlling the pH value of the fermented liquid after pre-treatment; electrodialysis to eliminate inorganic salt and regulating pH value again; leading in processed OH type anionic exchangecolumn to adsorb impurities and separate glutamine; decoloring, vacuum concentration, isoelectric crystallization, washing coarse crystal in organic solvent and drying. The said process has low exchange resin consumption, no conversion of glutamine in strong acid and strong alkali environment, low production cost and high total glutamine extracting rate up to 70%, and is suitable for industrial production.

The invention provides a technological method suitable for the industrialized purification of Carbetocin, a reversed-phase efficient liquid chromatography is used to purify the Carbetocin to result in high purity and good yield in order to meet industrialized demands. A crude peptide solution of the Carbetocin is filled with materials by a reversed-phase chromatographic column to be a stationary phase, a phosphate buffer solution is regarded as phase A and acetonitrile is regarded as phase B to implement gradient elution purification, wherein, the gradient: B%:20-40%, the pH of the phase A is 2.5-3.5; an anion exchange method is employed to convert the phosphate and trifluoroacetate to acetate. The invention employs one-step reversed-phase efficient liquid chromatography to purify followed by the acetate conversion by an anion exchange column in one step, thus obtaining high-purity acetate Carbetocin at high yield and offering an efficient purification technology for the massive purification and preparation of the peptide raw drugs.

The invention relates to a preparation method of RG-I type lycium barbarum pectin with anti-aging activity. The method comprises the following steps: (1) water is added into lycium barbarum, and the mixture is subjected to microwave-ultrasonic assisted extraction, filtering and concentration, such that a concentrate liquid is obtained; (2) anhydrous ethanol is added into the concentrate liquid, and the materials are well mixed; the mixture is allowed to stand and is subjected to centrifugation, such that a precipitation product is obtained; (3) the precipitation product is re-dissolved, and the solution is subjected to centrifugation and drying, such that lycium barbarum polysaccharide is obtained; (4) lycium barbarum polysaccharide subjected to DEAE-cellulose column chromatography separation, and is eluted; and dialysis desalination and lyophilization are carried out, such that lycium barbarum neutral polysaccharide LBP-N and lycium barbarum acidic polysaccharide LBP-A are respectively obtained; (5) lycium barbarum acidic polysaccharide LBP-A is subjected to Sepharose CL-6B gel filtration column chromatography separation, and is eluted; dialysis desalination and lyophilization are carried out, such that lycium barbarum acidic polysaccharides LBP-A-1 and LBP-A-2 are obtained; (6) lycium barbarum acidic polysaccharide LBP-A-1 is subjected to DEAE-Sepharpse Fast Flow anion exchange column chromatography purification; continuous gradient elution is carried out with a 0-0.5 mol/L NaCl aqueous solution; and dialysis desalination and lyophilization are carried out, such that the anti-aging RG-I type lycium barbarum pectin pure product is obtained. The method provided by the invention has the advantages of simple operation and high extraction rate

The extraction and separation method of algae polysaccharide is characterized by that the present invention utilzies the characteristics of polysaccharide and glucoprotein which are dissolved in water, adopts water extraction method to make extraction, uses ethyl alcohol to make fractional precipitation to make separation, finally adopts the anion-exchange column and gel exclusion chromatography to purity polysaccharide, in which the biological activity of protein and protein polysaccharide can be retained.

The invention discloses a preparation method of high-purity phycocyanin, and is characterized in that the preparation method comprises the following steps: (1) taking a fresh spirulina powder, fully mixing with a phosphate buffer solution evenly, repeatedly freezing and thawing for 7-10 times to break and remove cell walls, centrifuging to remove spirulina mud, and thus obtaining a supernatant; (2) adopting a two-step precipitation method with a 20%-30% ammonium sulfate and a 40%-60% ammonium sulfate to obtain a phycocyanin crude extract; (3) after dialyzing the crude extract, loading the sample onto a weak anion exchange column DEAE Sepharose FF, carrying out gradient elution after ion exchange, collecting an outflow component with A620/A280 of more than 3; and (4) dialyzing the collected sample, then loading the sample onto a strong anion exchange column SOURCE30Q, carrying out gradient elution after ion exchange, collecting an outflow component with A620/A280 of more than 4, again carrying out one-time ammonium sulfate precipitation concentration, and thus obtaining the high-purity phycocyanin having the purity of more than 4.5. The extraction purification method is simplified in process, wide in source of the raw material spirulina, simple in required equipment, and high in purity of the product, and has quite high application value.

The present invention is process of separating acetylpropionic acid with active carbon from the mixture solution of acetylpropionic acid and formic acid or acetylpropionic acid containing hydrolysate obtained through hydrolyzing sacchraide. The mixture solution or hydrolysate after being chromatographically separated in alkali cationic column and decolorized in macroporous resin is passed through adsorbing active carbon column to adsorb formic acid, acetylpropionic acid is eluted with water solution of alcohol, and the eluted solution is decompression concentrated to obtain high content acetylpropionic acid product. The present invention has complete separation of acetylpropionic acid from formic acid, high yield, low production cost, easy industrial application and other advantages.

The invention relates to a method for clean production of lactic acid by a calcium salt process, which comprises: regulating the pH value of fermentation liquid by using CaCO3; adding carbonate to perform a precipitation displacement reaction to obtain solution of soluble lactate and a CaCO3 precipitate; loading the solution of soluble lactate on an anion exchange column, so that lactates are absorbed onto the column and ion exchange permeate passing through the anion exchange column is obtained; eluting the anion exchange column with acid to obtain extrication liquid containing lactic acid; and subjecting the ion exchange permeate to bipolar membrane electrodialysis to obtain regenerated acid, alkali and desalted waste solution. The method for clean production of lactic acid by the calcium salt process completely overcome the drawback of production of calcium sulfate waste slag in production of lactic acid by the conventional production technique and can be connected with the conventional lactic acid fermentation step directly.

The invention discloses a method for extracting hirudin form leech saliva. The method includes the following steps of a), placing leeches in a container to allow the leeches to be hungered sufficiently, inputting inductive substances to the leeches to allow the same to absorb fully, adding vomitives to allow the leeches to spit the saliva in bodies, taking out blood-sucking leech living bodies to return to a rearing pond to feed continuously, and meanwhile, collecting hirudin crude product liquid in the container; b), freezing above hirudin crude products, adding into precooled cold acetone, stirring prior to placing in a refrigerator for setting overnight, sucking supernatant acetone the next day prior to centrifuging, adding a trichloroacetic acid solution, and centrifuging to remove residues to obtain concentrated liquid; c), eluting the above concentrated liquid with an anion exchange column chromatography method, and performing gel filtration chromatography to obtain finished products. The method has the advantages of reasonable process, convenience and practicability in operation, good quality stability of the finished hirudin products and high yield, one-time pillage on the leeches with a traditional method can be avoided, wild resources of the leeches are protected, and development of leech farming is driven.

The present invention relates to a method of purifying an antibody with high purity and high quality by removing impurities by sequential use of a cation-exchange column, a culture supernatant multilayer filter and an anion-exchange column without using a protein-A column that is an affinity chromatography column which is generally used for antibody purification.

The invention relates to a spirulina phatensis polysaccharide and an extraction method thereof. The extraction method comprises the following steps: after multigelation and wall breaking of a spirulina phatensis powder suspension, centrifuging to obtain a cell lysate 1 and cell debris; adding ammonium sulfate in the cell lysate 1 until the saturation is 50%, after salting-out precipitation, centrifuging to remove phycobiliprotein to obtain a supernatant, and evenly mixing the supernatant and the cell debris to obtain a cell lysate 2; obtaining a spirulina phatensis polysaccharide crude extract by using a hot water extraction method; adding the crude extract to an ethanol/ammonium sulfate aqueous two-phase system, and extracting to obtain a bottom-phase solution of spirulina phatensis polysaccharide with higher purity; after the bottom-phase solution is desalted by dialysis, eluting by using a Sephadex G-150 chromatographic column and a DEAE Sephadex A-50 anion exchange column to obtain a pure spirulina phatensis polysaccharide solution; and freeze-drying, thereby obtaining a pure spirulina phatensis polysaccharide finished product. The extraction method can be used for avoiding the traditional complicated protein removal operation steps, has low cost, high yield, and high purity and activity of polysaccharide, and is suitable for intermittent and large-scale production and processing.

The invention discloses a method for treating wastewater containing sodium sulfate with high concentration and chromium and a method for reclaiming resource, wherein the steps are: A) the wastewater containing chromium in industrial production is filtered to remove undissolved substance in the wastewater; B) filtrate is crystallized in a crystallizing pond to separate the sodium sulfate in the water; C) the filtrate respectively orderly passes through a positive ion exchange column and a negative ion exchange column to adsorb the useful components; D) after a saturated adsorption, the ion exchange columns are regenerated by desorbing agent; E) the desorbed liquid with high concentration can be used in an upper stream section of production, and the desorbed liquid with low concentration is used for preparing desorbing agent in the next batch for circulation and utilization. The chromium concentration in the wastewater containing the chromium treated by the method can be decreased to 0.5mg/L, and meets the national discharging standard; besides, the reclaiming rate of useful components is over 90 percent, thus realizing the treatment of wastewater and reclaiming of resources.

The invention discloses a zero-emission reuse treatment method for electroplating cyanide-containing wastewater and relates to a wastewater treatment technology. The wastewater is sent to a raw water regulation pool and then passes through a photocatalytic oxidation reactor and a membrane coagulation gas flotation reactor successively; back flushing outflow water of the membrane coagulation gas flotation reactor flows back to the raw water regulation pool; the outflow water of the membrane coagulation gas flotation reactor is sent to a continuous membrane filtration system for filtering, concentrated liquor of the continuous membrane filtration system and back-flushed water flow back to the raw water regulation pool; outflow water of the continuous membrane filtration system passes through a reverse osmosis system, an anion exchange column, an electro-adsorption reactor and a cation exchange column successively, and outflow water of the cation exchange column is recyclable purified water; eluted concentrated water of the electro-adsorption reactor and concentrated water of the reverse osmosis system are sent to a reverse osmosis concentrated water collecting pool, and outflow water enters a membrane contact reactor for cyanogen recovery; outflow water of the membrane contact reactor is sent to the electro-adsorption reactor, and metal electroplating liquid is obtained through evaporation, crystallization and concentration of eluted concentrated water; and outflow water of the electro-adsorption reactor is sent to the cation exchange column, and the outflow water is recyclable purified water.

The invention discloses a lumbricus polypeptide having anticoagulant and thrombolytic effects, and an enzymatic hydrolysis preparation method and an application thereof. The preparation method comprises the steps: cleaning and drying lumbricus dried bodies, and crushing into a powder; adding water, stirring, and centrifuging to obtain a protein precipitate; adding water to dissolve the protein, adding an alkaline protease with the ratio of the enzyme volume to the protein weight of 0.1-3 mL:100 g, and stirring; exterminating enzyme activity, adding trypsin with the ratio of the enzyme mass to the protein weight of 0.5-5 g:100 g, and stirring at the temperature of 25-60 DEG C; allowing the enzymatic hydrolysate to pass through an anion exchange column, collecting a part of eluate; and then passing through a macroporous adsorption resin, carrying out separation and purification, and collecting and drying to obtain a lumbricus anticoagulant peptide powder. A new source is added for high-activity anticoagulant active substances, the product activity is high, the preparation process is convenient and feasible, and the environment is protected; and the lumbricus polypeptide can be used as a raw material of foods for thrombosis prevention, thrombolysis and blood consistency reduction, is supplemented with lactose, mannitol and the like, and is made into a powder, a tablet, a capsule or an oral liquid.

The invention relates to an electronic grade citric acid and a production method thereof. In the citric acid, each metal ion is less than 0.5 mu g/g, heavy metal is less than 0.1 mug/g, Cl<-> is less than 1 mu g/g, SO4<2-> is less than 1 mu g/g, and the content of citric acid is not more than 99.5%. The production method comprises the following steps of: filtering a fermentation liquid from a fermentation procedure, extracting through neutralization and acidolysis procedures, further removing impurities of the extraction liquid through a carbon column, a cation exchange column and an anion exchange column to obtain an ion exchange liquid; alternatively, dissolving an industrial grade, food grade or medical grade citric acid product in electronic grade water to obtain a citric acid solution; leading the ion exchange liquid or the citric acid solution to pass through an cation exchange column and an anion exchange column loaded with specific resin, concentrating, precisely filtering, crystallizing, centrifuging, drying, screening and packaging to obtain the electronic grade citric acid. The electronic grade citric acid can be applied to the chemical etching and the chemical cleaningof very large scale integrated circuits, large screen liquid crystal displays and other microelectronics industries.

The invention discloses codonopsis pilosula uniform polysaccharide CPPib which is mainly composed of, by Mole percentage, 44.28% of rhamnose, 20.42% of galactose, 11.32% of arabinose and 23.97% of galacturonic acid, weight-average molecular weight of the codonopsis pilosula uniform polysaccharide CPPib is 1.45*105Da, and main carbohydrate chains are 1,2 connected rhamnose (rha, 45%), 1,4-connected galacturonic acid (GalA, 25%) and 1,2,6 connected galactose (Gal, 30%). A preparation method of the codonopsis pilosula uniform polysaccharide CPPib has the advantages of being simple, scientific, reasonable, and capable of preparing products with very high purity with only need of passing through an anion exchanging column chromatograph once. The prepared products have the advantages of having anti-tumor effect, educational effect, very high clinical application value and healthcare function.

The invention relates to a continuous preparation method for high-purity hydrogen peroxide. According to the continuous preparation method, industrial-grade hydrogen peroxide without a stabilizing agent is used a raw material. The continuous preparation method comprises the following steps which are sequentially and continuously operated: (1) adjusting the concentration of the hydrogen peroxide and controlling the temperature of the hydrogen peroxide between 0 and 15DEG C; (2) enabling the hydrogen peroxide to sequentially pass through a macroporous adsorption resin adsorption column, a cation exchange column and an anion exchange column at the flow speed of 300-450L/H and then circularly filtering the hydrogen peroxide with a filter element with 0.1 micrometer to obtain primarily-purified hydrogen peroxide; and (3) enabling the primarily-purified hydrogen peroxide to sequentially pass through a nanofiltration membrane with the aperture of 1-5 nanometers, a borosilicate resin adsorption column, a reverse osmosis membrane and mixed-bed resin at the flow speed of 300-450L/H and then circularly filtering the hydrogen peroxide with the filter element with 0.1 micrometer to obtain the high-purity hydrogen peroxide. According to the continuous preparation method disclosed by the invention, the high-purity hydrogen peroxide for preparing high-purity peracetic acid solution with no additive and favorable storage stability can be directly produced and lower production cost is obtained.

The present invention is one kind of protein extracted from Perinereis aibuhiteris and its preparation process and use. The protein is alkali protein with molecular weight 65000 Da and isoelectric point of 8.3. The protein preparing process includes raising Perinereis aibuhiteris in artificial sea water for 24-48 hr to make it become clean, electrically striking Perinereis aibuhiteris with 5-10 V DC to obtain body fluid, centrifugally filtering body fluid, reverse concentration, gel filtering, and chromatographic elution in anionic exchange column to collect active protein. The protein is used in preparing antibiotic medicine resisting escherichia coli, verdigris pseudomonad, staphylococcus aureus and arthrobacter and anticancer medicine for suppressing human liver cancer cell strain proliferation and producing alpha-fetoglobulin, and has high antibiotic activity and obvious anticancer effect.

The invention provides a method for producing xylose by use of xylose mother liquid, which comprises the following steps of: adding a certain amount of ion exchange liquid into the xylose mother liquid in xylose production to adjust the concentration of the dry matter of xylose liquid to 10-30%; enabling the mixed xylose liquid to pass through a filter membrane for intercepting 0.1-0.5kD molecular weight to intercept polysaccharide in the mixed xylose liquid and keep small-molecular monosaccharide in the filtrate; processing with a cation exchange column and an anion exchange column so as to control the content of cations and anions in the filtrate below 10ppm; and concentrating and crystallizing to obtain a finished product of xylose. By adding ion exchange liquid into the xylose mother liquid which is hard to crystallize, the purity of xylose in the xylose liquid is improved; the pigment and polysaccharide in the mixed xylose liquid are removed by membrane separation of a filter membrane, and the xylose liquid after the membrane filtration is concentrated; and since the polysaccharide component in the xylose liquid is removed, the xylose mother liquid hard to crystallize is crystallized and purified, the total crystallization rate of xylose is improved by about more than 10%, and the xylose production cost is greatly reduced.

The preparation and application of active foam protein for beer belongs to the field of beer foam stabilizer technology. Active foam protein with excellent solubility in beer environment is prepared with lacto albumin, soybean protein isolate, wheat mucedin and rice protein as material and through proper enzyme modification, ultrafiltering, anionic exchange column treatment and other steps. The present invention has active foam protein extracting process, high effect and low production cost, and the produced active foam protein can raise the foam stability of beer.

The invention discloses a method for preparing human blood coagulation factors IX and VII subcutaneously from cold-glue-removed blood plasma. The method comprises the following steps: 1, removing cold glue from blood plasma; 2, conducting strong anion-exchange column chromatography the first time; 3, conducting PEG sedimentation for removing impure protein; 4, conducting S/D viral inactivation; 5, conducting strong anion-exchange column chromatography the second time, and obtaining FVII eluent and FIX eluent; 6, conducting weak anion-exchange column chromatography, and concentration for purifying blood coagulation FVII; 7, conducting heparin affinity column chromatography for purifying blood coagulation FIX; 8, conducting ultrafiltration; 9, adding a stabilizing agent, and conducting adjustment; 10, conducting virus-removal filtration through nanofilms; 11, conducting sterilization, filtration and subpackage; 12, conducting freeze-drying; 13, conducting dry-hot viral inactivation. According to the method, PEG sedimentation is adopted for removing the impure protein, the target of preparing high-purity FVII and FIX simultaneously is achieved through combination of an ion-exchange column chromatography technology and an affinity chromatography technology, the process flow is simple, the production cycle is short, a product is subjected to three steps of virus eradicating measures, and use safety is high.