16 results about "Anion exchange column" patented technology

This invention belongs to the field of bile acid detection technology, and discloses a sample pretreatment method for determining the content of bile acid subtypes and its application. The pretreatment method includes the following steps: S1, acidifying the sample to be tested with an acidifying agent to a pH of 2-4 to obtain an acidified sample; S2, eluting the acidified sample onto a mixed-mode anion exchange column to obtain an eluent; S3, evaporating the eluent and reconstituted with a rehydration solution to obtain the test sample. The test sample obtained by this pretreatment method can effectively eliminate or compensate for matrix interference when used for targeted analysis of bile acid subtypes by LC-MS / MS, thus effectively improving detection sensitivity.

The application discloses a kind of acanthopanax acid polysaccharides with intestinal protection function and its preparation method and application, belong to the field of functional food and biotechnology.The acanthopanax acid polysaccharide is by water extraction alcohol precipitation, after impurity removal, through DEAE-52 anion exchange column elution and collection 0.2 mol / L NaCl elution peak, and the uniform component obtained by Sephadex G-100 gel column chromatography purification.Its uronic acid content is 30%-40%, weight average relative molecular weight is 50-100kDa, mainly by glucose, galactose, galacturonic acid and arabinose, infrared spectrum has carboxyl characteristic absorption peak at 1736 cm-1 and 1615 cm-1.Experiments prove that the acanthopanax acid polysaccharide has antioxidant activity and hypoglycemic activity.The acid polysaccharide has protective effect on LPS-induced Caco-2 cell damage, can effectively reduce the secretion of pro-inflammatory factors TNF-α, IL-1β, IL-6, and improve the level of anti-inflammatory factor IL-10.The acanthopanax acid polysaccharide can be prepared into oral liquid, tablet candy, solid beverage and other functional food forms.

This invention relates to the field of hydrometallurgical technology and discloses a method for purifying and recovering ammonium perrylate using a combination of electrodialysis and ion exchange. The method first adjusts the pH of the rhenium-containing feed solution, then selectively enriches perrylate ions using electrodialysis to obtain a high-concentration rhenium-rich solution. Subsequently, the rhenium-rich solution is subjected to deep adsorption purification through an anion exchange column. Next, rhenium is eluted from the saturated resin using an eluent to obtain a pure ammonium perrylate solution. Finally, high-purity ammonium perrylate crystals are obtained through evaporation, concentration, cooling, crystallization, and drying. Optionally, the obtained crystals can be recrystallized to further improve purity. This invention ensures the purity and process stability of the rhenium-rich solution obtained in the pre-enrichment stage, improves the overall adsorption capacity, desorption rate, and reliability of the process operation of the resin, and guarantees the stability of the final ammonium perrylate product purity and the economic benefits of the entire process recovery.

The application discloses radix sophorae tonkinensis polysaccharide STGP-1, a preparation method thereof and pharmaceutical use for treating lung squamous carcinoma, and relates to the technical field of medicines. The monosaccharide composition of STGP-1 includes fucose Fuc, arabinose Ara, galactose Gal, glucose Glc, xylose Xyl and mannose Man, and the molar ratio is 0.34:12.15:25.76:57.07:1.06:3.62. The STGP-1 is obtained by extraction, separation and purification from dried roots and rhizomes of radix sophorae tonkinensis, and the preparation method comprises the steps of pretreatment and crude extraction, impurity removal treatment, anion exchange column chromatography separation, gel column chromatography purification and the like. The STGP-1 can be used alone or in combination with anti-PD-1 monoclonal antibody for preparing a medicine for treating lung squamous carcinoma. The preparation process is stable, the source is natural, and the STGP-1 can provide a new high-efficiency and low-toxicity candidate medicine for immune combined treatment of lung squamous carcinoma.

The application discloses a Cs-4 polysaccharide extract quality detection method, and relates to the technical field of analysis and detection, and comprises the following steps: S1, acid hydrolysis is carried out on the Cs-4 polysaccharide extract, and after removing the acid, a hydrolyzate is obtained; S2, the hydrolyzate is subjected to anion exchange chromatography combined with pulse amperometric analysis, and a chromatogram is obtained; S3, a standard curve of a plurality of monosaccharides is prepared; S4, the chromatogram obtained in S2 is compared with the standard curve obtained in S3 and analyzed and calculated, and the components and contents of the monosaccharides are obtained. The ion chromatography method for separating monosaccharides by adopting an anion exchange column and detecting monosaccharides by a pulse amperometric detector directly analyzes the monosaccharide composition; the method does not need a derivatization reaction, has high sensitivity, and can realize comprehensive characterization of neutral, alkaline and acidic polysaccharides.

The application discloses a new application of Korla fragrant pear high-activity polysaccharide in promoting expression of small intestinal disaccharidase, and belongs to the technical field of functional food and medicine. In the application, Korla fragrant pear pulp is freeze-dried, ground, sieved, extracted with hot water, alcohol precipitated, decolorized and deproteinized, separated through a membrane filtration system, further purified through DEAE-Fast flow anion exchange column chromatography, and finally, Korla fragrant pear high-activity polysaccharide KPP-2-a is obtained. The application innovatively finds that KPP-2-a can effectively promote expression and activity of disaccharidase (lactase, sucrase and maltase) in the brush border of small intestinal mucosal epithelial cells, significantly improves secondary disaccharidase deficiency and related symptoms, excavates a new use of the Korla fragrant pear high-activity polysaccharide KPP-2-a and widens the application field thereof, provides a new means for intervention of secondary disaccharidase deficiency, and has a broad prospect and market value.

The invention provides a chromatographic method for separating pre-contraction state PVC Nanosyringes from post-contraction state PVC Nanosyringes, the method comprising: (a) providing a sample containing both pre- and post-contraction state PVC Nanosyringes; (b) loading the sample on a monolith anion exchange column having (preferably quaternary amine) binding moieties, under conditions (e.g. pH 7-8, temperature 19-25°C) wherein pre- and post-contraction state PVC Nanosyringes become bound to the column with differing affinities; (c) applying a gradient of ionic strength to the column that provides for differential elution of the pre- and post-contraction state PVC Nanosyringes, wherein the gradient is generated by incrementally (e.g. linearly) increasing the salt concentration of a mobile phase elution buffer that is applied to the column; and (d) eluting the pre- and post-contraction state PVC Nanosyringes into separate elution fractions under the gradient based on their differing affinities to the anion exchange column, and collecting fractions having the pre-contraction state PVC Nanosyringes separated from the post-contraction state PVC Nanosyringes.

This invention discloses a method for preparing a water-soluble homogeneous heteropolysaccharide from highland barley and its novel application in promoting the expression of β-galactosidase in the small intestinal mucosa, belonging to the field of polysaccharide preparation and application technology. This invention provides a method for preparing the water-soluble homogeneous heteropolysaccharide HBP-1a from highland barley, involving extraction, alcohol precipitation, decolorization, and deproteinization of highland barley raw materials, followed by separation and purification using anion exchange column chromatography and gel column chromatography. The water-soluble homogeneous heteropolysaccharide HBP-1a was successfully isolated from the highland barley polysaccharide. This invention is the first to discover that the water-soluble homogeneous heteropolysaccharide HBP-1a can effectively promote the expression of β-galactosidase in the small intestinal mucosa and can be used to improve symptoms related to lactose intolerance diarrhea. This invention explores a new use for the water-soluble homogeneous heteropolysaccharide HBP-1a from highland barley, broadening its application fields and possessing broad prospects and market value.

The application discloses a preparation process of special-coating small-particle-size high-purity high-stability silicon sol and relates to the technical field of nanometer material preparation. The preparation process specifically comprises the following steps: water glass with a certain modulus and mass concentration is sequentially passed through a cation exchange column, an anion exchange column and a cation exchange column to obtain an initial silicic acid solution; an alkaline catalyst is added into the initial silicic acid solution; the initial silicic acid solution is reacted for a certain time under specific temperature and stirring rate to form an initial silicon sol; and then, the initial silicon sol is concentrated into an aqueous silicon sol with a mass fraction of 20-40% of silicon dioxide; and the concentrated silicon sol is filtered to remove possible large particles. The silicon sol prepared in the application has the advantages of large specific surface area, small particle size, high purity and high stability, and has a good prospect in special coating.

This invention belongs to the field of plant polysaccharide technology, specifically relating to a Ganoderma lucidum solid-state fermented wheat pericarp polysaccharide with lipid-lowering activity and its preparation method. The polysaccharide is composed of fucose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid, and glucuronic acid, with molar percentages of 2.14%, 13.68%, 14.66%, 21.50%, 31.43%, 2.42%, 7.86%, and 6.31%, respectively, and an average molecular weight of 10.00×10³–15.00×10³ Da, preferably 11.05×10³ Da. This invention utilizes Ganoderma lucidum to ferment wheat pericarp in a solid state, followed by ultrasonic-assisted hot water extraction to crudely extract the polysaccharide. The crude polysaccharide is then purified by anion exchange column and dextran gel column chromatography. The resulting fermented wheat pericarp polysaccharide effectively improves lipid deposition, exhibits significant lipid-lowering activity, and has high safety.

This invention relates to the field of protein extraction technology, specifically disclosing a method for the simultaneous co-production and extraction of egg white lysozyme and ovotransferrin, comprising: (1) Egg white pretreatment: after dilution, adjust the pH to 4.5, filter out the ovomucin precipitate, and then adjust the supernatant to pH 8.0-9.0. (2) Cation exchange separation of lysozyme: adsorb onto a cation exchange column of the pretreatment solution, collect the permeate and eluent as the egg white flow-through solution; elute the lysozyme, and obtain the lysozyme product by concentration, desalting, sterilization, and drying. (3) Anion exchange separation of ovotransferrin: after adjusting the flow-through solution to pH 5.5-6.0, load onto an anion exchange column, elute to remove impurities, and collect the permeate containing ovotransferrin. (4) Iron removal and drying: concentrate by ultrafiltration, dialyze with citrate buffer for desalting and preliminary iron removal, then remove bound iron by chelating resin, and dry to obtain the iron-free ovotransferrin product.

The application discloses a kind of Sargassum fusiforme low molecular weight fucoidan, and its preparation method is as follows: S1, defatting treatment;S2, preparation of crude polysaccharide;S3, degradation of crude polysaccharide: Sargassum fusiforme fucoidan crude product is dissolved into polysaccharide solution with water, H2O2 is added, and degradation is carried out under ultraviolet light, to obtain Sargassum fusiforme low molecular weight fucoidan DSFF;S4, purification: Sargassum fusiforme low molecular weight fucoidan DSFF is dissolved into polysaccharide solution with water, loaded on DEAE-52 cellulose anion exchange column and eluted with NaCl solution, the eluate is concentrated and dialyzed, and freeze-drying is obtained.The application of the Sargassum fusiforme low molecular weight fucoidan in the preparation of drugs with anti-PM2.5 lung injury effect.The Sargassum fusiforme low molecular weight fucoidan of the application has low molecular weight, is easy to absorb, and has high bioavailability.The research of the application lays a foundation for the development of Sargassum fusiforme fucoidan related lung alveolus protection products.

This invention belongs to the field of biomedical technology and discloses a method for preparing sea buckthorn acidic polysaccharide with high rhamnose content and its application. The sea buckthorn acidic polysaccharide is a homogeneous acidic heteropolysaccharide with a weight average molecular weight of 1.00 × 10⁻⁶. 6 The total sugar content is 90.7%, with rhamnose accounting for 53.83 mol%. Its preparation method includes raw material pretreatment, hot water reflux extraction, ethanol precipitation, deproteinization and decolorization, and purification using a DEAE-52 anion exchange column and a Sephadex G-200 gel column. This invention provides a mild and reproducible preparation process, yielding a well-defined sea buckthorn acidic polysaccharide with a triple helix conformation. Furthermore, it can significantly alleviate ulcerative colitis damage, downregulate the expression levels of pro-inflammatory factors TNF-α and IL-6, and regulate intestinal flora homeostasis. It is suitable for preparing drugs, functional foods, and dietary supplements for the prevention and treatment of colitis.

The invention discloses a lotus leaf acidic homogeneous polysaccharide as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The polysaccharide is LLP-W with the molecular weight of 7.032 kDa or LLP-0.2 with the molecular weight of 18.774 kDa, the monosaccharide composition and molar ratio of the polysaccharide are clear, and the purity reaches 99% or above. The preparation method comprises the following steps: carrying out ethanol degreasing, hot water extraction and ethanol precipitation on lotus leaves to obtain a crude product, and then purifying through anion exchange column chromatography and gel column chromatography in sequence. The polysaccharide can effectively improve liver lipid deposition, and the mechanism of the polysaccharide relates to activation of GPR41 receptors through intestinal flora metabolites, so that lipid metabolism related signal channels are regulated and controlled, and fat accumulation is inhibited. The polysaccharide provided by the invention is clear in structure and definite in activity, and can be used for preparing medicines or health-care foods for preventing or treating obesity, non-alcoholic fatty liver and other metabolic diseases.

The application provides a method for detecting the purity of supercoiled DNA in plasmid samples with different molecular weights and application thereof, and comprises the following steps: preparing linearized plasmid samples and untreated plasmid samples for HPLC detection; performing HPLC detection by using an anion exchange column; collecting chromatograms of supercoiled plasmid DNA, open circle plasmid DNA, linear plasmid DNA and concatemer plasmid DNA in linearized and untreated plasmid samples with different molecular weights; and the mobile phase of the HPLC detection comprises: mobile phase A is a water solution containing tris-hydroxymethyl aminomethane, and mobile phase B is a water solution containing tris-hydroxymethyl aminomethane and sodium chloride. The method can ensure complete separation of supercoiled plasmid DNA, open circle plasmid DNA, linear plasmid DNA and concatemer plasmid DNA in plasmid samples with different molecular weights, has high resolution and high sensitivity, and has important application value in the field of nucleic acid drug analysis technology.

This invention discloses a method for simultaneously detecting sulfate ions and lithium salts in lithium battery electrolytes, belonging to the field of chemical analysis technology. The method includes: diluting the lithium battery electrolyte to be tested with water to obtain a test solution; and detecting the test solution using an ion chromatograph. The chromatographic conditions include: using an anion exchange column; eluent being a mixed aqueous solution containing sodium carbonate, sodium bicarbonate, and acetonitrile, wherein the concentration of sodium carbonate is 4.5–5.5 mmol / L, the concentration of sodium bicarbonate is 3.8–4.5 mmol / L, and the volume percentage of acetonitrile is 25–35%. The overall analytical process of this invention can be controlled within 30 minutes, and the detection limit is far below the industry standard requirements, achieving rapid and accurate simultaneous determination of multiple lithium salts and sulfate impurities in the electrolyte in a single ion chromatographic analysis.