19 results about "Maltase" patented technology

The invention discloses a method for detecting crude polysaccharide in an immunity-enhancing product, and relates to the technical field of detection, the immunity-enhancing product is taken and ground, water is added for mixing, freezing and heating drying are carried out, ultrasound is started while drying is carried out, pre-sample powder is obtained, water is added for boiling, then cooling is carried out, amylase and maltase are sequentially added for enzymolysis, enzyme deactivation and drying are carried out; sample powder is obtained; the method comprises the following steps: selecting a mixed solvent to extract sample powder, then adopting a basic cupric reagent to precipitate glucan, and then carrying out phenol-sulfuric acid reaction to obtain a to-be-detected solution; and calculating the crude polysaccharide content of the to-be-detected liquid according to the standard curve. According to the method, firstly, the sample is pretreated, that is, the sample is dissolved in water and then frozen, and then ultrasonic treatment is combined, so that sample particles are loosened and expanded, and subsequent dissolution in a solvent is facilitated. In addition, starch and dextrin are sequentially subjected to enzymolysis to form glucose, and then the glucose is precipitated, so that the influence of starch and dextrin auxiliary materials on polysaccharide is reduced, and the detection precision of crude polysaccharide is greatly improved.

The invention provides chickpea protein peptide as well as a preparation method and application thereof. The preparation method of the chickpea protein peptide provided by the invention comprises the following steps: sequentially carrying out enzymolysis on chickpea protein isolate by using neutral protease, pepsin and pancreatin; after the enzymolysis is finished, performing ultrafiltration on an enzymolysis product by using an ultrafiltration membrane with the interception amount of 3kDa, and collecting components with the molecular weight of less than 3kDa; and finally, carrying out gel chromatography on the component, and collecting the corresponding component to obtain the chickpea protein peptide. The chickpea protein peptide prepared by the method has inhibitory activity on DPP-IV, alpha-glucosidase and alpha-amylase, can effectively inhibit the activity of Caco-2 cell sucrase, maltase and DPP-IV, and has a certain inhibitory effect on glucose transport; therefore, the chickpea protein peptide provided by the invention has good hypoglycemic activity, and a new thought is provided for development of food-borne functional protein peptides.

Embodiments of the disclosure include particular amyloglucosidase (AMG) compositions formulated as a nutriceutical or medicinal food, for example. The AMG compositions are formulated at a specific dosage and / or are lacking in one or more toxins or have substantially reduced levels of toxin, such as deoxynivalenol (vomit toxin). The AMG compositions are provided to individuals in need thereof, such as an individual with or at risk for congenital sucrase isomaltase syndrome, functional bowel disorders, small bowel bacterial overgrowth, protein-calorie malnutrition (marasmus), radiochemotherapy-induced mucositis and / or short-gut syndrome.

The application discloses application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and belongs to the technical field of feed additives. The application provides application of Fagopyrum cymosum root powder in preparation of products for improving egg quality or intestinal health of laying ducks, and the Fagopyrum cymosum root powder can improve eggshell strength, yolk color and eggshell proportion, improve the content of aspartic acid, threonine, glutamic acid, glycine, cystine and leucine in yolk, improve the content of arachidonic acid and arachidonic acid in yolk, and reduce the content of saturated fatty acid in yolk; the Fagopyrum cymosum root powder can improve the activity of maltase in the mucous membrane of the jejunum of laying ducks, and can improve the diversity and abundance of intestinal flora of laying ducks. The application provides a new strategy for improving egg quality or intestinal health of laying ducks.

The present invention relates to an aqueous adhesive composition comprising: - a) starch treated with a starch maltase or maltodextrin treated with a starch maltase; and - b) ammonium zirconium carbonate.

The invention provides a skin acne-removing and anti-inflammatory preparation and a preparation method thereof, and belongs to the technical field of cosmetics. The preparation is prepared from the following raw materials in percentage by mass: 15 to 30 percent of acne-removing and anti-inflammatory functional components, 0.5 to 20 percent of emollient, 0.5 to 15 percent of emulsifier, 1 to 25 percent of filler, 1 to 15 percent of thickener, 0.05 to 0.3 percent of preservative, 0.01 to 0.5 percent of pH regulator, 0.005 to 0.05 percent of essence and the balance of water, the acne-removing and anti-inflammatory functional components are obtained by sequentially performing enzymolysis and fermentation on traditional Chinese medicine raw materials; the traditional Chinese medicine raw materials comprise radix paeoniae rubra, seaweed, honeysuckle, dandelion and rose; a composite enzymolysis agent is used for enzymolysis, and the composite enzymolysis agent comprises beta-galactosidase, coenzyme A, laccase and maltase; a composite leavening agent is used for fermentation, and the composite leavening agent comprises xylanolyticus, saccharopolyspora spinosa, bacillus licheniformis and candida tropicalis. The preparation prepared by the invention is reasonable in formula composition, not only has excellent acne-removing and anti-inflammatory effects, but also has certain stability.

The present disclosure relates to antisense oligomers and related compositions and methods for inducing exon inclusion as a treatment for glycogen storage disease type II (GSD-II) (also known as Pompe disease, glycogenosis II, acid maltase deficiency (AMD), acid alpha-glucosidase deficiency, and lysosomal alpha-glucosidase deficiency), and more specifically relates to inducing inclusion of exon 2 and thereby restoring levels of enzymatically active acid alpha-glucosidase (GAA) protein encoded by the GAA gene.

The application discloses a new application of Korla fragrant pear high-activity polysaccharide in promoting expression of small intestinal disaccharidase, and belongs to the technical field of functional food and medicine. In the application, Korla fragrant pear pulp is freeze-dried, ground, sieved, extracted with hot water, alcohol precipitated, decolorized and deproteinized, separated through a membrane filtration system, further purified through DEAE-Fast flow anion exchange column chromatography, and finally, Korla fragrant pear high-activity polysaccharide KPP-2-a is obtained. The application innovatively finds that KPP-2-a can effectively promote expression and activity of disaccharidase (lactase, sucrase and maltase) in the brush border of small intestinal mucosal epithelial cells, significantly improves secondary disaccharidase deficiency and related symptoms, excavates a new use of the Korla fragrant pear high-activity polysaccharide KPP-2-a and widens the application field thereof, provides a new means for intervention of secondary disaccharidase deficiency, and has a broad prospect and market value.

The invention belongs to the technical field of biological medicines, and particularly relates to a culicoides streptonicoides allergen as well as an identification method, an antibody and application thereof. The culicoides chromogen is maltase Cel t 1, and the amino acid sequence of the maltase Cel t 1 is as shown in SEQ NO. 1. According to the application disclosed by the invention, the maltase Curet 1 obtained by screening is used as the culicoides chromogen, so that the defect of single research object of blood-sucking midges in the early stage can be overcome, and a good foundation is laid for subsequent blood-sucking midge chromogen research. Meanwhile, one of sensitizers in the culicoides is defined on the basis of the culicoides-mouse sensitization model constructed through early-stage research, an efficient technical method is provided for research on identification of the culicoides sensitizers, and meanwhile basic data is provided for subsequent vaccine prevention and treatment research.

The invention relates to the technical field of bacillus licheniformis solid fermentation culture medium, in particular to a bacillus licheniformis solid fermentation culture medium, which is composed of the following components: 10-30 weight parts of composite active enzyme, 120-140 weight parts of potato peel paste, 230-240 weight parts of sheep manure, 15-25 weight parts of yeast powder, 1-3 weight parts of potassium dihydrogen phosphate, 4-6 weight parts of magnesium sulfate, 0.1-0.3 weight parts of manganese sulfate, 40-50 weight parts of calcium carbonate, 8-12 weight parts of sodium chloride and 25-35 weight parts of beef extract, wherein the composite active enzyme is prepared by mixing alpha-amylase and maltase at a mass ratio of 1:0.5-2 and then being embedded and modified by sodium alginate, and the composite active enzyme is used to interact with the potato peel paste to prepare a bacillus licheniformis solid fermentation culture medium, which can provide a more suitable growth environment and nutrients required for growth for microorganisms, and effectively improve the number of live bacteria of the bacillus licheniformis.

The invention discloses an application of wild buckwheat root powder in preparation of a product for improving duck egg quality or intestinal health, and belongs to the technical field of feed additives. The invention provides an application of wild buckwheat root powder in preparation of a product for improving duck egg quality or intestinal health. The wild buckwheat root powder can improve eggshell strength, yolk color and eggshell proportion; the contents of asparaginic acid, threonine, glutamic acid, glycine, cystine and leucine in the egg yolk are increased; the content of arachidonic acid and arachidonic acid in the egg yolk is increased, and the content of saturated fatty acid in the egg yolk is reduced; the activity of egg duck jejunum mucosa maltase is improved; and the diversity and abundance of intestinal flora of laying ducks can be improved. The invention provides a new strategy for improving the quality of egg-laying duck eggs or intestinal health.

The invention discloses application of siRNA in a postprandial hyperglycemia treatment preparation, and belongs to the technical field of biology. A siRNA sequence for silencing maltase-glucoamylase protein expression is designed by analyzing mRNA sequences of human and mouse maltase-glucoamylase, and unmodified siRNA and 2 '-OMe, 2'-F basic group modified siRNA are respectively synthesized. The two kinds of siRNA transfect human small intestinal mucosal epithelial cells through lipidosome, the silence efficiency of the unmodified siRNA on the MGAM gene is 81.54%, and the silence efficiency of the base modified siRNA on the MGAM gene is 82.09%. When the two siRNAs are intravenously injected into a type 2 diabetes mellitus model mouse, the expression of maltase-glucoamylase can be effectively silenced, postprandial hyperglycemia is reduced, the stability of the base modified siRNA is improved, the drug effect time can be prolonged, and the medication frequency can be reduced.

Feed additive for an avian granivore comprising a malt extract comprising one or more enzymes selected from amylases, maltases, cellulases, fructanases, glucanases, xylanases, and deacetylases. The fe

The invention discloses a sugar source degrading microorganism and a construction method and application thereof, the recombinant microorganism is characterized in that glucoamylase and / or maltase are / is introduced into the microorganism by using a molecular modification technology, so that related enzymes expressed by the microorganism can continuously hydrolyze disaccharide or polysaccharide in a sugar source into glucose in a fermentation process; microorganisms can simultaneously utilize various sugar sources for fermentation, the utilization rate of the sugar sources is improved, and the fermentation cost is reduced.

The invention relates to the field of extraction of effective components of natural plants, in particular to a bacterium-enzyme combination method for fermentation of Chinese wolfberry fruits and an application of the bacterium-enzyme combination method. The lycium barbarum polysaccharide is obtained by mixing lycium barbarum and water and then sequentially performing homogenization, enzymolysis, enzyme deactivation, fermentation, filtration and purification. Cellulase, maltase and amylase are adopted as a compound enzyme preparation, the cellulase firstly destroys cell walls to enable other components (polysaccharide, starch and protein) to be dissolved out, the amylase catalyzes the starch to be decomposed into the maltase, the maltase further catalyzes the maltose to be decomposed into glucose, and due to the addition of the amylase, the content of the maltose in the starch is reduced. The fermentation efficiency can also be improved. The streptococcus amylases, the streptococcus thermophilus and the bifidobacterium longum are used as the complex microbial inoculants, the streptococcus amylases can effectively decompose starch and non-starch polysaccharides, the streptococcus thermophilus and the bifidobacterium longum can further decompose other substances, the Chinese wolfberry fruits are fully utilized, and finally the purpose of improving the extraction rate and the purity of the Chinese wolfberry fruit polysaccharides is achieved.

The invention belongs to the field of food processing, and particularly relates to a white hyacinth bean protein beverage and a preparation method thereof. The hyacinth bean protein beverage comprises the following components: cooked white hyacinth bean powder, sodium alginate and chlorogenic acid. The preparation method comprises the following steps: adding alpha-amylase, maltase and cellulase, carrying out enzymolysis, and carrying out colloid milling, homogenization, sterilization and cooling. The white hyacinth bean protein beverage prepared by the method is unique in flavor, fine and smooth in taste, free of obvious beany flavor and free of layering after standing. And the production process is simple, easy to control and suitable for industrial production.

The application discloses application of siRNA in preparation of postprandial hyperglycemia treatment preparation and belongs to the technical field of biology. By analyzing maltase-glucoamylase mRNA sequences of human and mice, siRNA sequences for silencing maltase-glucoamylase protein expression are designed, and unmodified siRNA and siRNA with 2'-OMe and 2'-F base modification are synthesized. The two kinds of siRNA are transfected into human small intestinal mucosal epithelial cells by liposomes, the silencing efficiency of the unmodified siRNA on the MGAM gene is 81.54%, and the silencing efficiency of the siRNA with base modification on the MGAM gene is 82.09%. The two kinds of siRNA are injected intravenously into type 2 diabetes model mice, and the expression of maltase-glucoamylase can be effectively silenced, postprandial hyperglycemia is reduced, the stability of the siRNA with base modification is improved, the drug efficacy time can be prolonged, and the drug use frequency is reduced.

This invention discloses a modified maltase adjuvant and its preparation method. The preparation method includes: dissolving maltase in phosphate buffer, adding a bifunctional coupling agent (N-hydroxysuccinimide-polyethylene glycol-dibenzocyclooctylene) for activation; then covalently grafting the activated maltase with a hydrophilic modifier and a functional small molecule (1-carboxybutyl-3-methylimidazolium salt); finally, purifying by ultrafiltration to obtain the modified maltase adjuvant. This invention constructs a stable covalent structure on maltase containing both hydrophilic segments and functional ionic liquids through sequential functionalization modification mediated by a specific coupling agent. The resulting adjuvant possesses excellent enzyme stability enhancement and catalytic auxiliary functions, significantly improving the thermal stability, catalytic efficiency, and reusability of various industrial enzymes. It exhibits the characteristics of "multiple effects in one agent" and strong versatility, making it suitable for multiple fields such as biocatalysis, food processing, and bioenergy.

The invention belongs to the technical field of biological medicines, and particularly relates to application of 1, 3-dicaffeoylquinic acid (1, 3-DCQA) in preparation of a medicine for improving glucose and lipid metabolism disorder. The invention discloses application of 1, 3-DCQA in nitraria tangutorum bobr to preparation of a medicine for improving glucose and lipid metabolism disorder, and proves that the 1, 3-DCQA is inhibited by glycosidase (sucrase and maltase) of the 1, 3-DCQA, so that intestinal flora is changed, then short-chain fatty acid acetic acid secretion is promoted, acetic acid is used as a signal molecule to activate a GPR43 signal channel, and further insulin sensitivity is improved. The effect of regulating glycolipid metabolism is achieved. The invention not only provides a solid experimental basis for medicinal development of 1, 3-DCQA in Nitraria tangutorum Bobr, but also provides a brand new action target and direction for research and development of novel medicaments for treating glucose and lipid metabolism disorders, and has important theoretical significance and wide clinical transformation prospects.